Rh6AG187100

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0009241
Physical Location & Seq
Forward (+)
33087427 .. 33092981
5555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG187100.1

Sequence Viewer

Length: 1338 bp
ATGCTATTTTTATCAAAATATGGGAAAGAATTTGTATCAGCTGCAACAGAGCTAATGCCAGATTGTGGTGTCAGTCGCCAGTTGATAGAACTGCTATCTGTTCGGGCTCCTTCAGCTGAGAAAAAGCTGAAGCTTTTGAAAGAAATTGCTGAAGAGCATGAACTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATTTTCAAACCGCATGAGGATTTGCTGAATGGTCCGACACAGTTTGTCAGCGGGTCTAAGTTGCCGCTTCCCGAAGAAAAACATGATGAGACGTTGTACCGTGCACCTGATCAAGCTCAGAAAGAACAGTCTGATTCTGATGATGGTTTGGACGTTTTAGACTTTCCGGAAGTTCCGAAGGTATCATCAAGACCAACTGAGAGTATACCCTCAGCACCAACAATGGCTCCACCTTTTCCACCCCCACCACACCCTGATTTTGATCACGAGTTATCAAAAAATTCTGAGGCGATTGGACATCCATCCAAAGAGCCGTCCTTTGAGTCTGGAGAAGAAAGAGCAATGGCAGACAAGCATGAATCTCATGCTTCAGTTGGTCATGGGGATGATGTACAGTTTTTGCCATTTATATCTCCTCCATCTCTTTCATCTGCATCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTCAGCTGCTGCTCAGGCTGCTGCAGATTCTGCAGCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTTAGCTGCTGCTCAGGCTGCTGCAGATTCGGCAGAACGTGCGGCAGCAGCAGCTCGCTCAGCAGCTAGTCTTGCACAGGTAAGAATAAATGAGCTGACCAAGAAAAACAATGAAGAAGTATCTGAAAGTAGCTGTGAAAACCCATTTCATGAAGACGTTCCTAATCAGTCAGCTACTAAAGAAAAGTCGGATTTTGATCATCATATCCCCGATGGTGATTCTAGTGGTGTTATAAATTCTCCAGAACCCCATCAAGGCCATGAATATGGCCGGGGATCAGAGACATCAAATCTTTCGTTTAAGGACTCGGCTAAAGTGGATTTTGATTCCCCGCTTGCCAGCGATAATGTTTTTGAACATGAGCCAGCTCATCACCAGCCTCAGAGATTGACTTCAATGGATGATGACCCATACTTCTCGTACCCAAACTTATTCACATCAGAAAACAATGTTGGATCTGGTGCTCAGTCTTCTACAGATAATTCTCGCTTCTCGCATGAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

445

Amino Acids

48.46

Weight (kDa)

4.76

Isoelectric Point (pI)

54.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 2 - 55 9.8e-11 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1064
AccB7I CCANNNNNTGG 1 cut(s) 65
AccI GTMKAC 1 cut(s) 400
AccIII TCCGGA 1 cut(s) 361
AciI CCGC 5 cut(s) 206, 246, 260, 872, 1163
AclWI GGATC 4 cut(s) 167, 180, 1114, 1294
AcoI YGGCCR 1 cut(s) 1099
AcsI RAATTY 4 cut(s) 29, 195, 475, 1066
AcuI CTGAAG 5 cut(s) 96, 149, 171, 207, 549
AfaI GTAC 3 cut(s) 293, 588, 1253
AfiI CCNNNNNNNGG 2 cut(s) 65, 1085
AgsI TTSAA 6 cut(s) 139, 202, 675, 792, 1187, 1227
AjuI GAANNNNNNNTTGG 2 cut(s) 1266, 1298
Alw21I GWGCWC 2 cut(s) 301, 1297
Alw26I GTCTC 4 cut(s) 278, 637, 754, 1106
Alw44I GTGCAC 1 cut(s) 297
AlwI GGATC 4 cut(s) 167, 180, 1114, 1294
AlwNI CAGNNNCTG 2 cut(s) 722, 743
Aor13HI TCCGGA 1 cut(s) 361
AoxI GGCC 2 cut(s) 1087, 1099
ApaLI GTGCAC 1 cut(s) 297
ApoI RAATTY 4 cut(s) 29, 195, 475, 1066
Asp700I GAANNNNTTC 1 cut(s) 987
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 1 cut(s) 1103
AsuHPI GGTGA 2 cut(s) 1058, 1196
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 1 cut(s) 301
BamHI GGATCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 109
BauI CACGAG 1 cut(s) 461
BbsI GAAGAC 2 cut(s) 990, 1293
Bbv12I GWGCWC 2 cut(s) 301, 1297
BbvCI CCTCAGC 1 cut(s) 406
BccI CCATC 5 cut(s) 332, 505, 622, 1037, 1089
BceAI ACGGC 1 cut(s) 493
BclI TGATCA 3 cut(s) 304, 457, 1027
BcnI CCSGG 1 cut(s) 1103
BcoDI GTCTC 4 cut(s) 278, 637, 754, 1106
BfaI CTAG 2 cut(s) 897, 1053
BfmI CTRYAG 4 cut(s) 735, 744, 852, 1305
BlpI GCTNAGC 1 cut(s) 889
Bme1390I CCNGG 1 cut(s) 1103
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 3 cut(s) 108, 174, 423
BmrFI CCNGG 1 cut(s) 1103
BmsI GCATC 2 cut(s) 191, 638
BpiI GAAGAC 2 cut(s) 990, 1293
BpmI CTGGAG 2 cut(s) 543, 1056
Bpu10I CCTNAGC 3 cut(s) 406, 726, 843
Bpu1102I GCTNAGC 1 cut(s) 889
BpuMI CCSGG 1 cut(s) 1103
BsaBI GATNNNNATC 3 cut(s) 171, 456, 1026
BsaJI CCNNGG 1 cut(s) 1102
BsaWI WCCGGW 1 cut(s) 361
BsaXI ACNNNNNCTCC 2 cut(s) 406, 436
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 1085
Bse1I ACTGG 2 cut(s) 79, 168
Bse3DI GCAATG 1 cut(s) 543
Bse8I GATNNNNATC 3 cut(s) 171, 456, 1026
BseAI TCCGGA 1 cut(s) 361
BseDI CCNNGG 1 cut(s) 1102
BseGI GGATG 4 cut(s) 493, 497, 586, 1237
BseJI GATNNNNATC 3 cut(s) 171, 456, 1026
BseLI CCNNNNNNNGG 2 cut(s) 65, 1085
BseMI GCAATG 1 cut(s) 543
BseNI ACTGG 2 cut(s) 79, 168
BseRI GAGGAG 1 cut(s) 600
BseSI GKGCMC 1 cut(s) 301
BshFI GGCC 2 cut(s) 1089, 1101
BsiHKAI GWGCWC 2 cut(s) 301, 1297
BsiSI CCGG 2 cut(s) 362, 1102
BslI CCNNNNNNNGG 2 cut(s) 65, 1085
BsmAI GTCTC 4 cut(s) 278, 637, 754, 1106
BsmBI CGTCTC 1 cut(s) 278
BsnI GGCC 2 cut(s) 1089, 1101
Bsp1286I GDGCHC 3 cut(s) 109, 301, 1297
Bsp13I TCCGGA 1 cut(s) 361
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 6 cut(s) 172, 304, 457, 1027, 1106, 1286
Bsp1720I GCTNAGC 1 cut(s) 889
BspACI CCGC 5 cut(s) 206, 246, 260, 872, 1163
BspANI GGCC 2 cut(s) 1089, 1101
BspEI TCCGGA 1 cut(s) 361
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 3 cut(s) 108, 174, 423
BspMAI CTGCAG 3 cut(s) 739, 748, 856
BspPI GGATC 4 cut(s) 167, 180, 1114, 1294
BspQI GCTCTTC 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 543
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 2 cut(s) 79, 168
BssECI CCNNGG 1 cut(s) 1102
BssMI GATC 6 cut(s) 172, 304, 457, 1027, 1106, 1286
BssNAI GTATAC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 461
Bst1107I GTATAC 1 cut(s) 401
Bst2BI CACGAG 1 cut(s) 461
Bst4CI ACNGT 4 cut(s) 237, 296, 324, 591
Bst6I CTCTTC 1 cut(s) 147
BstAPI GCANNNNNTGC 2 cut(s) 743, 869
BstAUI TGTACA 1 cut(s) 586
BstC8I GCNNGC 4 cut(s) 886, 1167, 1171, 1197
BstF5I GGATG 4 cut(s) 493, 497, 586, 1237
BstKTI GATC 6 cut(s) 175, 307, 460, 1030, 1109, 1289
BstMAI GTCTC 4 cut(s) 278, 637, 754, 1106
BstMBI GATC 6 cut(s) 172, 304, 457, 1027, 1106, 1286
BstSCI CCNGG 1 cut(s) 1101
BstSFI CTRYAG 4 cut(s) 735, 744, 852, 1305
BstSLI GKGCMC 1 cut(s) 301
BstV2I GAAGAC 2 cut(s) 990, 1293
BstX2I RGATCY 2 cut(s) 172, 1286
BstXI CCANNNNNNTGG 1 cut(s) 1097
BstYI RGATCY 2 cut(s) 172, 1286
BstZ17I GTATAC 1 cut(s) 401
BsuRI GGCC 2 cut(s) 1089, 1101
BtsCI GGATG 4 cut(s) 493, 497, 586, 1237
BtsIMutI CAGTG 1 cut(s) 1333
Cac8I GCNNGC 4 cut(s) 886, 1167, 1171, 1197
CaiI CAGNNNCTG 2 cut(s) 722, 743
CciI TCATGA 1 cut(s) 979
Cfr13I GGNCC 1 cut(s) 227
Csp6I GTAC 3 cut(s) 292, 587, 1252
CviQI GTAC 3 cut(s) 292, 587, 1252
DpnI GATC 6 cut(s) 174, 306, 459, 1029, 1108, 1288
DpnII GATC 6 cut(s) 172, 304, 457, 1027, 1106, 1286
EaeI YGGCCR 1 cut(s) 1099
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 5 cut(s) 96, 149, 171, 207, 549
EcoT38I GRGCYC 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 278
FauI CCCGC 2 cut(s) 239, 1170
FbaI TGATCA 3 cut(s) 304, 457, 1027
FblI GTMKAC 1 cut(s) 400
FokI GGATG 4 cut(s) 480, 484, 593, 1244
FriOI GRGCYC 1 cut(s) 109
FspBI CTAG 2 cut(s) 897, 1053
GsuI CTGGAG 2 cut(s) 543, 1056
HaeIII GGCC 2 cut(s) 1089, 1101
HapII CCGG 2 cut(s) 362, 1102
HindIII AAGCTT 1 cut(s) 131
HpaII CCGG 2 cut(s) 362, 1102
HphI GGTGA 2 cut(s) 1058, 1196
Hpy166II GTNNAC 2 cut(s) 299, 401
Hpy188III TCNNGA 9 cut(s) 266, 362, 384, 461, 522, 675, 792, 980, 1073
Hpy8I GTNNAC 2 cut(s) 299, 401
HpyAV CCTTC 2 cut(s) 120, 367
HpyCH4III ACNGT 4 cut(s) 237, 296, 324, 591
HpyCH4IV ACGT 4 cut(s) 287, 348, 868, 987
HpySE526I ACGT 4 cut(s) 287, 348, 868, 987
Kpn2I TCCGGA 1 cut(s) 361
Ksp22I TGATCA 3 cut(s) 304, 457, 1027
Kzo9I GATC 6 cut(s) 172, 304, 457, 1027, 1106, 1286
LguI GCTCTTC 1 cut(s) 147
LmnI GCTCC 2 cut(s) 112, 427
LweI GCATC 2 cut(s) 191, 638
MaeI CTAG 2 cut(s) 897, 1053
MaeII ACGT 4 cut(s) 287, 348, 868, 987
MalI GATC 6 cut(s) 174, 306, 459, 1029, 1108, 1288
MboI GATC 6 cut(s) 172, 304, 457, 1027, 1106, 1286
MboII GAAGA 8 cut(s) 164, 281, 539, 690, 807, 956, 995, 1293
MflI RGATCY 2 cut(s) 172, 1286
MhlI GDGCHC 3 cut(s) 109, 301, 1297
MluCI AATT 6 cut(s) 29, 144, 195, 475, 1066, 1312
MlyI GAGTC 2 cut(s) 527, 1130
MmeI TCCRAC 3 cut(s) 254, 999, 1264
MnlI CCTC 9 cut(s) 205, 415, 475, 621, 676, 682, 793, 799, 1221
MroI TCCGGA 1 cut(s) 361
MroXI GAANNNNTTC 1 cut(s) 987
MseI TTAA 1 cut(s) 1131
MslI CAYNNNNRTG 2 cut(s) 579, 1095
MspA1I CMGCKG 4 cut(s) 41, 116, 246, 719
MspI CCGG 2 cut(s) 362, 1102
MspR9I CCNGG 1 cut(s) 1103
NciI CCSGG 1 cut(s) 1103
NdeII GATC 6 cut(s) 172, 304, 457, 1027, 1106, 1286
NlaIV GGNNCC 3 cut(s) 108, 174, 423
NmeAIII GCCGAG 1 cut(s) 1118
PagI TCATGA 1 cut(s) 979
PciSI GCTCTTC 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 987
PfeI GAWTC 8 cut(s) 329, 554, 652, 740, 769, 857, 1049, 1157
PflMI CCANNNNNTGG 1 cut(s) 65
PleI GAGTC 2 cut(s) 526, 1130
PpsI GAGTC 2 cut(s) 526, 1130
PsiI TTATAA 1 cut(s) 1064
PspN4I GGNNCC 3 cut(s) 108, 174, 423
PspPI GGNCC 1 cut(s) 227
PstI CTGCAG 3 cut(s) 739, 748, 856
PstNI CAGNNNCTG 2 cut(s) 722, 743
PsuI RGATCY 2 cut(s) 172, 1286
PvuII CAGCTG 3 cut(s) 41, 116, 719
RsaI GTAC 3 cut(s) 293, 588, 1253
RsaNI GTAC 3 cut(s) 292, 587, 1252
RseI CAYNNNNRTG 2 cut(s) 579, 1095
SapI GCTCTTC 1 cut(s) 147
SaqAI TTAA 1 cut(s) 1131
Sau3AI GATC 6 cut(s) 172, 304, 457, 1027, 1106, 1286
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 2 cut(s) 527, 1130
ScrFI CCNGG 1 cut(s) 1103
SduI GDGCHC 3 cut(s) 109, 301, 1297
SfaNI GCATC 2 cut(s) 191, 638
SfcI CTRYAG 4 cut(s) 735, 744, 852, 1305
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 579, 1095
Sse9I AATT 6 cut(s) 29, 144, 195, 475, 1066, 1312
SsiI CCGC 5 cut(s) 206, 246, 260, 872, 1163
SspMI CTAG 2 cut(s) 897, 1053
StyD4I CCNGG 1 cut(s) 1101
TaaI ACNGT 4 cut(s) 237, 296, 324, 591
TaiI ACGT 4 cut(s) 290, 351, 871, 990
TasI AATT 6 cut(s) 29, 144, 195, 475, 1066, 1312
TatI WGTACW 1 cut(s) 586
TauI GCSGC 2 cut(s) 262, 875
TfiI GAWTC 8 cut(s) 329, 554, 652, 740, 769, 857, 1049, 1157
Tru1I TTAA 1 cut(s) 1131
Tru9I TTAA 1 cut(s) 1131
TspDTI ATGAA 7 cut(s) 174, 567, 612, 957, 968, 996, 1107
Van91I CCANNNNNTGG 1 cut(s) 65
VneI GTGCAC 1 cut(s) 297
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 4 cut(s) 29, 195, 475, 1066
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 987
XspI CTAG 2 cut(s) 897, 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.