Rroxscaffold_7G00195920

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009241
Physical Location & Seq
Reverse (-)
38510414 .. 38516335
5922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00195920.1

Sequence Viewer

Length: 1821 bp
ATGTCGATGCTCGATTCCTTCTTCAACAAGGGCTTCAAAGCTGCCAAATGCAAAACTCTGCTGAAATTGACGATTCCGCGGATAAAGTTGTTGAGGAACAGGAGAGAAATTCAGATTAGACAGATGCGCCGCGACATTGCTAAGCTTCTTGAGACTGGCCAGGAAGCTACAGCTCGGATTCGGGTAGAGCATATTATTAGAGAAGAGAATATGATGGCAGCTCAGGAGATCATCGAGCTATATGCTGAGCTTATTTCTGTCCGCCTTCCAATAGTTGAATCACAAAGGGAGTGCCCTCTAGACTTGAAAGAAGCAATATCCAGTGTGTGTTTTGCTGCACCAAGATGCGCTGATCTGCCGGAGTTGCTTCAAGTTCAAATGCTATTTGTATCTAAATATGGGAAAGAATTTGTATCAGCAGCAACAGAGCTAATGCCAGATTGTGGTGTCAATCGCCAGTTGATAGAGCTGCTATCTGTTCGGGCTCCTTCAGCTGAGAAAAAGCTGAAGCTTTTGAAAGAAATTGCTGAAGAGCATGAACTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATTTTCAAACCGCATGAGGATTTGCTGAATGGTCCGACACAGTTTGTCAGCGGGTCTAAGTTGCCGCTTCCCGAAGAAAAACATGATGAGACGTTGTACTGTGCACCTGATCAAGCTCAGAAAGAACAGTCTGATTCTGATGATGGTTCGGACGTTTCAGACTTTCCGGAAGTTCCGAAGGTATCATTAAGACCAACTGAGAGTATACCCTCAGCACCAGCGATGGCTCCACCTTTTCCACCCCCACCACACCCTGATTTTGATCATGAGTTATCAAACAATTCTGAGGCGATTGGACATCCATCCAAAGAGCCGTCCTTTGAGTCTGGAGAAGAAAGAGCAATGGCAGACAAGCATGAATCTCATGCTTCAGTTGGTCGTGGGGATGATGTACAGTTTTTGCCATTTATATCTCCCCCATCTCTTTCGTCTGCATCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTCAGCGGCTGCAGATTCTGCAGCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTCAGCTGCTGCTCAGGCTGCTGCAGATTCTGCAGCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAATGAGGTCAATGTAGATATGCAAGATGTGTTAGCTGCTGCTCAGGCTGCTGCAGATTCGGCAGAACGTGCGGCAGCAGCAGCTCGCTCAGCAGCTAGTCTTGCACAGGTAAGAATAAATGAGCTGACCAAGAAAAACAATGAAGAAGTATCTGAAAGTAGCTGTGAAAACCCATTTCATGAAGACGTTCCTAATCAGTCTGCTACTAAAGAAAAGTCGGATTTTGATCATCATGTCCCCGATGCTGATTCTAGTGGTGTTATAAATTCTCCAGAACCTCATCAAGGCCATGAATATGGCCGGGGATCAGAGACATCAAATCTTTCGTTTAAGGACTCGGCTAAAGTGGATTTTGATTCCCCGCTTCCCAGCGATAATGTTTTTGAACACGAGCCAGCTCATCACCAGCCTCAGAGATTGACTTCAATGGATGATGACCCATACTTCTCGTACCCAAACTTATTCACATCAGAAAACAATGTTGGATCTGTTGCTCAGTCTTCTACAGATAATTCTCGCTTCTCGCATGAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

606

Amino Acids

66.74

Weight (kDa)

4.99

Isoelectric Point (pI)

55.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 3.8e-59 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1547
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 1 cut(s) 778
AccII CGCG 2 cut(s) 79, 132
AccIII TCCGGA 1 cut(s) 739
AclWI GGATC 4 cut(s) 545, 558, 1597, 1777
AcoI YGGCCR 2 cut(s) 157, 1582
AcsI RAATTY 4 cut(s) 108, 407, 573, 1549
AcuI CTGAAG 5 cut(s) 474, 527, 549, 585, 927
AfaI GTAC 3 cut(s) 671, 966, 1736
AfiI CCNNNNNNNGG 2 cut(s) 443, 1568
AjnI CCWGG 1 cut(s) 159
AjuI GAANNNNNNNTTGG 2 cut(s) 1749, 1781
Alw21I GWGCWC 1 cut(s) 679
Alw26I GTCTC 6 cut(s) 146, 656, 1015, 1120, 1237, 1589
Alw44I GTGCAC 1 cut(s) 675
AlwI GGATC 4 cut(s) 545, 558, 1597, 1777
AlwNI CAGNNNCTG 4 cut(s) 1100, 1109, 1205, 1226
Aor13HI TCCGGA 1 cut(s) 739
AoxI GGCC 3 cut(s) 157, 1570, 1582
ApaLI GTGCAC 1 cut(s) 675
ApoI RAATTY 4 cut(s) 108, 407, 573, 1549
Asp700I GAANNNNTTC 1 cut(s) 1470
AspLEI GCGC 2 cut(s) 129, 350
AspS9I GGNCC 1 cut(s) 605
AsuC2I CCSGG 1 cut(s) 1586
AsuHPI GGTGA 1 cut(s) 1679
AvaII GGWCC 1 cut(s) 605
BaeGI GKGCMC 2 cut(s) 296, 679
BalI TGGCCA 1 cut(s) 159
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 487
BauI CACGAG 1 cut(s) 1673
BbsI GAAGAC 2 cut(s) 1473, 1776
Bbv12I GWGCWC 1 cut(s) 679
BbvCI CCTCAGC 1 cut(s) 784
BccI CCATC 5 cut(s) 208, 710, 790, 883, 1000
BceAI ACGGC 1 cut(s) 871
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 1 cut(s) 161
BclI TGATCA 3 cut(s) 682, 835, 1510
BcnI CCSGG 1 cut(s) 1586
BcoDI GTCTC 6 cut(s) 146, 656, 1015, 1120, 1237, 1589
BfaI CTAG 3 cut(s) 299, 1380, 1536
BfmI CTRYAG 7 cut(s) 168, 1101, 1110, 1218, 1227, 1335, 1788
BlpI GCTNAGC 3 cut(s) 141, 246, 1372
Bme1390I CCNGG 2 cut(s) 161, 1586
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 3 cut(s) 486, 552, 801
BmrFI CCNGG 2 cut(s) 161, 1586
BmsI GCATC 5 cut(s) 114, 335, 569, 1016, 1516
BpiI GAAGAC 2 cut(s) 1473, 1776
BpmI CTGGAG 2 cut(s) 921, 1539
Bpu10I CCTNAGC 4 cut(s) 222, 784, 1209, 1326
Bpu1102I GCTNAGC 3 cut(s) 141, 246, 1372
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 1 cut(s) 1586
BsaBI GATNNNNATC 3 cut(s) 549, 834, 1509
BsaJI CCNNGG 2 cut(s) 77, 1585
BsaWI WCCGGW 1 cut(s) 739
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 1568
Bse1I ACTGG 4 cut(s) 160, 321, 457, 546
Bse3DI GCAATG 2 cut(s) 135, 921
Bse8I GATNNNNATC 3 cut(s) 549, 834, 1509
BseAI TCCGGA 1 cut(s) 739
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 2 cut(s) 77, 1585
BseGI GGATG 4 cut(s) 871, 875, 964, 1720
BseJI GATNNNNATC 3 cut(s) 549, 834, 1509
BseLI CCNNNNNNNGG 2 cut(s) 443, 1568
BseMI GCAATG 2 cut(s) 135, 921
BseNI ACTGG 4 cut(s) 160, 321, 457, 546
BseSI GKGCMC 2 cut(s) 296, 679
BseYI CCCAGC 1 cut(s) 1652
BsgI GTGCAG 1 cut(s) 321
Bsh1236I CGCG 2 cut(s) 79, 132
BshFI GGCC 3 cut(s) 159, 1572, 1584
BsiHKAI GWGCWC 1 cut(s) 679
BsiSI CCGG 3 cut(s) 359, 740, 1585
BslFI GGGAC 1 cut(s) 1505
BslI CCNNNNNNNGG 2 cut(s) 443, 1568
BsmAI GTCTC 6 cut(s) 146, 656, 1015, 1120, 1237, 1589
BsmBI CGTCTC 1 cut(s) 656
BsmFI GGGAC 1 cut(s) 1505
BsnI GGCC 3 cut(s) 159, 1572, 1584
Bsp1286I GDGCHC 3 cut(s) 296, 487, 679
Bsp13I TCCGGA 1 cut(s) 739
Bsp1407I TGTACA 1 cut(s) 964
Bsp143I GATC 8 cut(s) 228, 352, 550, 682, 835, 1510, 1589, 1769
Bsp1720I GCTNAGC 3 cut(s) 141, 246, 1372
BspANI GGCC 3 cut(s) 159, 1572, 1584
BspEI TCCGGA 1 cut(s) 739
BspFNI CGCG 2 cut(s) 79, 132
BspHI TCATGA 2 cut(s) 838, 1462
BspLI GGNNCC 3 cut(s) 486, 552, 801
BspMAI CTGCAG 5 cut(s) 1105, 1114, 1222, 1231, 1339
BspPI GGATC 4 cut(s) 545, 558, 1597, 1777
BspQI GCTCTTC 1 cut(s) 525
BsrDI GCAATG 2 cut(s) 135, 921
BsrGI TGTACA 1 cut(s) 964
BsrI ACTGG 4 cut(s) 160, 321, 457, 546
BssECI CCNNGG 2 cut(s) 77, 1585
BssMI GATC 8 cut(s) 228, 352, 550, 682, 835, 1510, 1589, 1769
BssNAI GTATAC 1 cut(s) 779
BssSI CACGAG 1 cut(s) 1673
Bst1107I GTATAC 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 1673
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 4 cut(s) 615, 674, 702, 969
Bst6I CTCTTC 2 cut(s) 198, 525
BstAPI GCANNNNNTGC 3 cut(s) 1109, 1226, 1352
BstAUI TGTACA 1 cut(s) 964
BstC8I GCNNGC 2 cut(s) 1369, 1680
BstDSI CCRYGG 1 cut(s) 77
BstENI CCTNNNNNAGG 1 cut(s) 1566
BstF5I GGATG 4 cut(s) 871, 875, 964, 1720
BstFNI CGCG 2 cut(s) 79, 132
BstHHI GCGC 2 cut(s) 129, 350
BstKTI GATC 8 cut(s) 231, 355, 553, 685, 838, 1513, 1592, 1772
BstMAI GTCTC 6 cut(s) 146, 656, 1015, 1120, 1237, 1589
BstMBI GATC 8 cut(s) 228, 352, 550, 682, 835, 1510, 1589, 1769
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 2 cut(s) 159, 1584
BstSFI CTRYAG 7 cut(s) 168, 1101, 1110, 1218, 1227, 1335, 1788
BstSLI GKGCMC 2 cut(s) 296, 679
BstUI CGCG 2 cut(s) 79, 132
BstV2I GAAGAC 2 cut(s) 1473, 1776
BstX2I RGATCY 2 cut(s) 550, 1769
BstXI CCANNNNNNTGG 1 cut(s) 1580
BstYI RGATCY 2 cut(s) 550, 1769
BstZ17I GTATAC 1 cut(s) 779
BsuRI GGCC 3 cut(s) 159, 1572, 1584
BtgI CCRYGG 1 cut(s) 77
BtgZI GCGATG 1 cut(s) 809
BtsCI GGATG 4 cut(s) 871, 875, 964, 1720
BtsIMutI CAGTG 2 cut(s) 328, 1816
Cac8I GCNNGC 2 cut(s) 1369, 1680
CaiI CAGNNNCTG 4 cut(s) 1100, 1109, 1205, 1226
CciI TCATGA 2 cut(s) 838, 1462
CfoI GCGC 2 cut(s) 129, 350
Cfr13I GGNCC 1 cut(s) 605
Cfr42I CCGCGG 1 cut(s) 80
Csp6I GTAC 3 cut(s) 670, 965, 1735
CviQI GTAC 3 cut(s) 670, 965, 1735
DpnI GATC 8 cut(s) 230, 354, 552, 684, 837, 1512, 1591, 1771
DpnII GATC 8 cut(s) 228, 352, 550, 682, 835, 1510, 1589, 1769
EaeI YGGCCR 2 cut(s) 157, 1582
Eam1104I CTCTTC 2 cut(s) 198, 525
EarI CTCTTC 2 cut(s) 198, 525
EciI GGCGGA 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 605
Eco57I CTGAAG 5 cut(s) 474, 527, 549, 585, 927
EcoNI CCTNNNNNAGG 1 cut(s) 1566
EcoRII CCWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 487
Esp3I CGTCTC 1 cut(s) 656
FaqI GGGAC 1 cut(s) 1505
FauI CCCGC 2 cut(s) 617, 1653
FbaI TGATCA 3 cut(s) 682, 835, 1510
FblI GTMKAC 1 cut(s) 778
FokI GGATG 4 cut(s) 858, 862, 971, 1727
FriOI GRGCYC 1 cut(s) 487
FspBI CTAG 3 cut(s) 299, 1380, 1536
GlaI GCGC 2 cut(s) 128, 349
GsaI CCCAGC 1 cut(s) 1656
GsuI CTGGAG 2 cut(s) 921, 1539
HaeIII GGCC 3 cut(s) 159, 1572, 1584
HapII CCGG 3 cut(s) 359, 740, 1585
HhaI GCGC 2 cut(s) 129, 350
Hin6I GCGC 2 cut(s) 127, 348
HinP1I GCGC 2 cut(s) 127, 348
HindIII AAGCTT 2 cut(s) 143, 509
HpaII CCGG 3 cut(s) 359, 740, 1585
HphI GGTGA 1 cut(s) 1679
Hpy166II GTNNAC 2 cut(s) 677, 779
Hpy8I GTNNAC 2 cut(s) 677, 779
HpyAV CCTTC 4 cut(s) 28, 275, 498, 745
HpyCH4III ACNGT 4 cut(s) 615, 674, 702, 969
HpyCH4IV ACGT 4 cut(s) 665, 726, 1351, 1470
HpySE526I ACGT 4 cut(s) 665, 726, 1351, 1470
HspAI GCGC 2 cut(s) 127, 348
Kpn2I TCCGGA 1 cut(s) 739
Ksp22I TGATCA 3 cut(s) 682, 835, 1510
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 8 cut(s) 228, 352, 550, 682, 835, 1510, 1589, 1769
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 2 cut(s) 490, 805
LweI GCATC 5 cut(s) 114, 335, 569, 1016, 1516
MaeI CTAG 3 cut(s) 299, 1380, 1536
MaeII ACGT 4 cut(s) 665, 726, 1351, 1470
MalI GATC 8 cut(s) 230, 354, 552, 684, 837, 1512, 1591, 1771
MboI GATC 8 cut(s) 228, 352, 550, 682, 835, 1510, 1589, 1769
MflI RGATCY 2 cut(s) 550, 1769
MhlI GDGCHC 3 cut(s) 296, 487, 679
MlsI TGGCCA 1 cut(s) 159
MluCI AATT 8 cut(s) 65, 108, 407, 522, 573, 853, 1549, 1795
MluNI TGGCCA 1 cut(s) 159
MlyI GAGTC 2 cut(s) 905, 1613
MmeI TCCRAC 3 cut(s) 632, 1482, 1747
Mox20I TGGCCA 1 cut(s) 159
MroI TCCGGA 1 cut(s) 739
MroXI GAANNNNTTC 1 cut(s) 1470
MscI TGGCCA 1 cut(s) 159
MseI TTAA 2 cut(s) 761, 1614
MslI CAYNNNNRTG 2 cut(s) 343, 1578
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 5 cut(s) 79, 494, 624, 1097, 1202
MspI CCGG 3 cut(s) 359, 740, 1585
MspR9I CCNGG 2 cut(s) 161, 1586
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 2 cut(s) 79, 132
NciI CCSGG 1 cut(s) 1586
NdeII GATC 8 cut(s) 228, 352, 550, 682, 835, 1510, 1589, 1769
NlaIV GGNNCC 3 cut(s) 486, 552, 801
NmeAIII GCCGAG 1 cut(s) 1601
PagI TCATGA 2 cut(s) 838, 1462
PciSI GCTCTTC 1 cut(s) 525
PcsI WCGNNNNNNNCGW 1 cut(s) 1007
PdmI GAANNNNTTC 1 cut(s) 1470
PflMI CCANNNNNTGG 1 cut(s) 443
PleI GAGTC 2 cut(s) 904, 1613
PpsI GAGTC 2 cut(s) 904, 1613
PsiI TTATAA 1 cut(s) 1547
Psp6I CCWGG 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 1652
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 3 cut(s) 486, 552, 801
PspPI GGNCC 1 cut(s) 605
PstI CTGCAG 5 cut(s) 1105, 1114, 1222, 1231, 1339
PstNI CAGNNNCTG 4 cut(s) 1100, 1109, 1205, 1226
PsuI RGATCY 2 cut(s) 550, 1769
PvuII CAGCTG 2 cut(s) 494, 1202
RsaI GTAC 3 cut(s) 671, 966, 1736
RsaNI GTAC 3 cut(s) 670, 965, 1735
RseI CAYNNNNRTG 2 cut(s) 343, 1578
SacII CCGCGG 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 2 cut(s) 761, 1614
Sau3AI GATC 8 cut(s) 228, 352, 550, 682, 835, 1510, 1589, 1769
Sau96I GGNCC 1 cut(s) 605
SchI GAGTC 2 cut(s) 905, 1613
ScrFI CCNGG 2 cut(s) 161, 1586
SduI GDGCHC 3 cut(s) 296, 487, 679
SfaNI GCATC 5 cut(s) 114, 335, 569, 1016, 1516
SfcI CTRYAG 7 cut(s) 168, 1101, 1110, 1218, 1227, 1335, 1788
Sfr303I CCGCGG 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 2 cut(s) 343, 1578
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 8 cut(s) 65, 108, 407, 522, 573, 853, 1549, 1795
SspMI CTAG 3 cut(s) 299, 1380, 1536
StyD4I CCNGG 2 cut(s) 159, 1584
TaaI ACNGT 4 cut(s) 615, 674, 702, 969
TaiI ACGT 4 cut(s) 668, 729, 1354, 1473
TaqI TCGA 3 cut(s) 5, 12, 234
TasI AATT 8 cut(s) 65, 108, 407, 522, 573, 853, 1549, 1795
TatI WGTACW 2 cut(s) 669, 964
TauI GCSGC 4 cut(s) 132, 640, 1100, 1358
Tru1I TTAA 2 cut(s) 761, 1614
Tru9I TTAA 2 cut(s) 761, 1614
TscAI CASTG 1 cut(s) 328
TspDTI ATGAA 6 cut(s) 552, 945, 1440, 1451, 1479, 1590
TspRI CASTG 1 cut(s) 328
Van91I CCANNNNNTGG 1 cut(s) 443
VneI GTGCAC 1 cut(s) 675
VpaK11BI GGWCC 1 cut(s) 605
XagI CCTNNNNNAGG 1 cut(s) 1566
XapI RAATTY 4 cut(s) 108, 407, 573, 1549
XbaI TCTAGA 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 778
XmnI GAANNNNTTC 1 cut(s) 1470
XspI CTAG 3 cut(s) 299, 1380, 1536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.