Rh6DG181200

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009241
Physical Location & Seq
Forward (+)
32042992 .. 32048097
5106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG181200.1

Sequence Viewer

Length: 1584 bp
ATGTCGATGCTCGATTCCTTCTTCAACAAGGGCTTCAAAGCTGCCAAATGGGAGTGCCCTCTAGACTTGAAAGAAGCAATATCCAGTCTGTGTTTTGCTGCACCAAGATGCGCTGATCTGCCGGAGTTGCTTCAAGTTCAAATGCTATTTGTATCTAAATATGGGAAAGAATTTGTATCAGCTGCAACAGAGCTAATGCCAGATTGTGGTGTCAGTCGCCAGTTGATAGAGCTGCTATCTGTTCGGGCTCCTTCAGCTGAGAAAAAGCTGAAGCTTTTGAAAGAAATTGCTGAAGAGCATGAACTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATTTTCAAACCGCATGAGGATTTGCTGAATGGTCCGACACAGTTTGTCAGCGGGTCTAAGTTGCCGCTTCCCGAAGAAAAACATGATGAGACGTTGTACTGTGCACCTGATCAAGCTCAGAAAGAACAGTCTGATTCTGATGATGGTTTGGACGTTTTAGACTTTCCGGAAGTTCCGAAGGTATCATCAAGACCAACTGAGAGTATACTCTCAGCACCAGCAATGGCTCCACCTTTTCCACCCCCACCACACCCTGATTTTGATCACGAGTTATCAAAAAATTCTGAGGCGATTGGACATCCATCCAAAGAGCCGTCCTTTGAGTCTGGAGAAGAAAGAGCAATGGCAGACAAGCATGAATCTCATGCTTCAGTTGGTCATGGGGATGATGTACAGTTTTTGCCATTTATATCTCCTCCATCTCTTTCATCTGCATCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTCAGCTGCTGCTCAGGCTGCTGCAGATTCTGCAGCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAAAGTAGATATGCAAGATGTGTCAGCTGCTGCAGATTCTGCAGTGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTTAGCTGCTGCTCAGGCTGCTGCAGATTCGGCAGAACGTGCGGCAGCAGCAGCTCGCTCAGCAGCTAGTCTTGCACAGGTAAGAATAAATGAGCTGACCAAGAAAAACAATGAAGAAGTATCTGAAAGTAGCTGTGAAAACCCATTTCATGAAGACGTTCCTAATCAGTCAGCTACTGGAGAAAAGTCGGATTTTGATCATCATATCCCTGATGGTGATTCTAGTGGTGTTATAAATTCTCCAGAACCCCATCAAGGCCATGGATATGGCCGGGGATCAGAGACATCAAATCTTTCGTTTAAGGACTCGGCTAAAGTGGATTTTGATTCCCCGCTTCCCAGCGATAATGTTTTTGAACACGAGCCAGCTCATCACCAGCCTCAGAGATTGACTTCAATGGATGATGACCCATACTTCTCGTACCCAAACTTATTCACATCAGAAAACAATGTTGGATCTGGTGCTCAGTCTTCTACAGATAATTCTCGCTTCTCGCATGAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

527

Amino Acids

57.27

Weight (kDa)

4.79

Isoelectric Point (pI)

55.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 17 - 102 2.2e-26 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1310
AccB7I CCANNNNNTGG 1 cut(s) 206
AccI GTMKAC 1 cut(s) 541
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 5 cut(s) 347, 387, 401, 1118, 1409
AclWI GGATC 4 cut(s) 308, 321, 1360, 1540
AcoI YGGCCR 1 cut(s) 1345
AcsI RAATTY 4 cut(s) 170, 336, 616, 1312
AcuI CTGAAG 5 cut(s) 237, 290, 312, 348, 690
AfaI GTAC 3 cut(s) 434, 729, 1499
AfiI CCNNNNNNNGG 2 cut(s) 206, 1331
AjuI GAANNNNNNNTTGG 2 cut(s) 1512, 1544
Alw21I GWGCWC 2 cut(s) 442, 1543
Alw26I GTCTC 5 cut(s) 419, 778, 895, 1000, 1352
Alw44I GTGCAC 1 cut(s) 438
AlwI GGATC 4 cut(s) 308, 321, 1360, 1540
AlwNI CAGNNNCTG 5 cut(s) 863, 884, 980, 989, 1253
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 2 cut(s) 1333, 1345
ApaLI GTGCAC 1 cut(s) 438
ApoI RAATTY 4 cut(s) 170, 336, 616, 1312
Asp700I GAANNNNTTC 1 cut(s) 1233
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 1 cut(s) 368
AsuC2I CCSGG 1 cut(s) 1349
AsuHPI GGTGA 2 cut(s) 1304, 1442
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 2 cut(s) 59, 442
BamHI GGATCC 1 cut(s) 313
BanII GRGCYC 1 cut(s) 250
BauI CACGAG 2 cut(s) 602, 1436
BbsI GAAGAC 2 cut(s) 1236, 1539
Bbv12I GWGCWC 2 cut(s) 442, 1543
BccI CCATC 5 cut(s) 473, 646, 763, 1283, 1335
BceAI ACGGC 1 cut(s) 634
BclI TGATCA 3 cut(s) 445, 598, 1273
BcnI CCSGG 1 cut(s) 1349
BcoDI GTCTC 5 cut(s) 419, 778, 895, 1000, 1352
BfaI CTAG 3 cut(s) 62, 1143, 1299
BfmI CTRYAG 6 cut(s) 876, 885, 981, 990, 1098, 1551
BlpI GCTNAGC 1 cut(s) 1135
Bme1390I CCNGG 1 cut(s) 1349
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 3 cut(s) 249, 315, 564
BmrFI CCNGG 1 cut(s) 1349
BmsI GCATC 3 cut(s) 98, 332, 779
BpiI GAAGAC 2 cut(s) 1236, 1539
BpmI CTGGAG 3 cut(s) 684, 1275, 1302
Bpu10I CCTNAGC 2 cut(s) 867, 1089
Bpu1102I GCTNAGC 1 cut(s) 1135
BpuMI CCSGG 1 cut(s) 1349
BsaBI GATNNNNATC 3 cut(s) 312, 597, 1272
BsaJI CCNNGG 2 cut(s) 1336, 1348
BsaWI WCCGGW 1 cut(s) 502
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 1331
Bse1I ACTGG 4 cut(s) 84, 220, 309, 1258
Bse3DI GCAATG 2 cut(s) 564, 684
Bse8I GATNNNNATC 3 cut(s) 312, 597, 1272
BseAI TCCGGA 1 cut(s) 502
BseDI CCNNGG 2 cut(s) 1336, 1348
BseGI GGATG 4 cut(s) 634, 638, 727, 1483
BseJI GATNNNNATC 3 cut(s) 312, 597, 1272
BseLI CCNNNNNNNGG 2 cut(s) 206, 1331
BseMI GCAATG 2 cut(s) 564, 684
BseNI ACTGG 4 cut(s) 84, 220, 309, 1258
BseRI GAGGAG 1 cut(s) 741
BseSI GKGCMC 2 cut(s) 59, 442
BseYI CCCAGC 1 cut(s) 1415
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 2 cut(s) 1335, 1347
BsiHKAI GWGCWC 2 cut(s) 442, 1543
BsiSI CCGG 3 cut(s) 122, 503, 1348
BslI CCNNNNNNNGG 2 cut(s) 206, 1331
BsmAI GTCTC 5 cut(s) 419, 778, 895, 1000, 1352
BsmBI CGTCTC 1 cut(s) 419
BsnI GGCC 2 cut(s) 1335, 1347
Bsp1286I GDGCHC 4 cut(s) 59, 250, 442, 1543
Bsp13I TCCGGA 1 cut(s) 502
Bsp1407I TGTACA 1 cut(s) 727
Bsp143I GATC 7 cut(s) 115, 313, 445, 598, 1273, 1352, 1532
Bsp1720I GCTNAGC 1 cut(s) 1135
Bsp19I CCATGG 1 cut(s) 1336
BspACI CCGC 5 cut(s) 347, 387, 401, 1118, 1409
BspANI GGCC 2 cut(s) 1335, 1347
BspEI TCCGGA 1 cut(s) 502
BspHI TCATGA 1 cut(s) 1225
BspLI GGNNCC 3 cut(s) 249, 315, 564
BspMAI CTGCAG 5 cut(s) 880, 889, 985, 994, 1102
BspPI GGATC 4 cut(s) 308, 321, 1360, 1540
BspQI GCTCTTC 1 cut(s) 288
BsrDI GCAATG 2 cut(s) 564, 684
BsrGI TGTACA 1 cut(s) 727
BsrI ACTGG 4 cut(s) 84, 220, 309, 1258
BssECI CCNNGG 2 cut(s) 1336, 1348
BssMI GATC 7 cut(s) 115, 313, 445, 598, 1273, 1352, 1532
BssNAI GTATAC 1 cut(s) 542
BssSI CACGAG 2 cut(s) 602, 1436
BssT1I CCWWGG 1 cut(s) 1336
Bst1107I GTATAC 1 cut(s) 542
Bst2BI CACGAG 2 cut(s) 602, 1436
Bst4CI ACNGT 4 cut(s) 378, 437, 465, 732
Bst6I CTCTTC 1 cut(s) 288
BstAPI GCANNNNNTGC 3 cut(s) 884, 989, 1115
BstAUI TGTACA 1 cut(s) 727
BstC8I GCNNGC 2 cut(s) 1132, 1443
BstDSI CCRYGG 1 cut(s) 1336
BstF5I GGATG 4 cut(s) 634, 638, 727, 1483
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 7 cut(s) 118, 316, 448, 601, 1276, 1355, 1535
BstMAI GTCTC 5 cut(s) 419, 778, 895, 1000, 1352
BstMBI GATC 7 cut(s) 115, 313, 445, 598, 1273, 1352, 1532
BstSCI CCNGG 1 cut(s) 1347
BstSFI CTRYAG 6 cut(s) 876, 885, 981, 990, 1098, 1551
BstSLI GKGCMC 2 cut(s) 59, 442
BstV2I GAAGAC 2 cut(s) 1236, 1539
BstX2I RGATCY 2 cut(s) 313, 1532
BstXI CCANNNNNNTGG 1 cut(s) 1343
BstYI RGATCY 2 cut(s) 313, 1532
BstZ17I GTATAC 1 cut(s) 542
BsuRI GGCC 2 cut(s) 1335, 1347
BtgI CCRYGG 1 cut(s) 1336
BtsCI GGATG 4 cut(s) 634, 638, 727, 1483
BtsI GCAGTG 1 cut(s) 999
BtsIMutI CAGTG 2 cut(s) 999, 1579
Cac8I GCNNGC 2 cut(s) 1132, 1443
CaiI CAGNNNCTG 5 cut(s) 863, 884, 980, 989, 1253
CciI TCATGA 1 cut(s) 1225
CfoI GCGC 1 cut(s) 113
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 3 cut(s) 433, 728, 1498
CviAII CATG 9 cut(s) 299, 350, 419, 692, 701, 716, 1226, 1337, 1574
CviQI GTAC 3 cut(s) 433, 728, 1498
DpnI GATC 7 cut(s) 117, 315, 447, 600, 1275, 1354, 1534
DpnII GATC 7 cut(s) 115, 313, 445, 598, 1273, 1352, 1532
EaeI YGGCCR 1 cut(s) 1345
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 1336
Eco24I GRGCYC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 368
Eco57I CTGAAG 5 cut(s) 237, 290, 312, 348, 690
EcoT14I CCWWGG 1 cut(s) 1336
EcoT38I GRGCYC 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 1336
Esp3I CGTCTC 1 cut(s) 419
FaeI CATG 9 cut(s) 302, 353, 422, 695, 704, 719, 1229, 1340, 1577
FatI CATG 9 cut(s) 298, 349, 418, 691, 700, 715, 1225, 1336, 1573
FauI CCCGC 2 cut(s) 380, 1416
FbaI TGATCA 3 cut(s) 445, 598, 1273
FblI GTMKAC 1 cut(s) 541
FokI GGATG 4 cut(s) 621, 625, 734, 1490
FriOI GRGCYC 1 cut(s) 250
FspBI CTAG 3 cut(s) 62, 1143, 1299
GlaI GCGC 1 cut(s) 112
GsaI CCCAGC 1 cut(s) 1419
GsuI CTGGAG 3 cut(s) 684, 1275, 1302
HaeIII GGCC 2 cut(s) 1335, 1347
HapII CCGG 3 cut(s) 122, 503, 1348
HhaI GCGC 1 cut(s) 113
Hin1II CATG 9 cut(s) 302, 353, 422, 695, 704, 719, 1229, 1340, 1577
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HindIII AAGCTT 1 cut(s) 272
HpaII CCGG 3 cut(s) 122, 503, 1348
HphI GGTGA 2 cut(s) 1304, 1442
Hpy166II GTNNAC 2 cut(s) 440, 542
Hpy8I GTNNAC 2 cut(s) 440, 542
HpyAV CCTTC 3 cut(s) 28, 261, 508
HpyCH4III ACNGT 4 cut(s) 378, 437, 465, 732
HpyCH4IV ACGT 4 cut(s) 428, 489, 1114, 1233
HpySE526I ACGT 4 cut(s) 428, 489, 1114, 1233
Hsp92II CATG 9 cut(s) 302, 353, 422, 695, 704, 719, 1229, 1340, 1577
HspAI GCGC 1 cut(s) 111
Kpn2I TCCGGA 1 cut(s) 502
Ksp22I TGATCA 3 cut(s) 445, 598, 1273
Kzo9I GATC 7 cut(s) 115, 313, 445, 598, 1273, 1352, 1532
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 2 cut(s) 253, 568
LweI GCATC 3 cut(s) 98, 332, 779
MaeI CTAG 3 cut(s) 62, 1143, 1299
MaeII ACGT 4 cut(s) 428, 489, 1114, 1233
MalI GATC 7 cut(s) 117, 315, 447, 600, 1275, 1354, 1534
MboI GATC 7 cut(s) 115, 313, 445, 598, 1273, 1352, 1532
MflI RGATCY 2 cut(s) 313, 1532
MhlI GDGCHC 4 cut(s) 59, 250, 442, 1543
MluCI AATT 6 cut(s) 170, 285, 336, 616, 1312, 1558
MlyI GAGTC 2 cut(s) 668, 1376
MmeI TCCRAC 3 cut(s) 395, 1245, 1510
MroI TCCGGA 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 1233
MseI TTAA 1 cut(s) 1377
MslI CAYNNNNRTG 3 cut(s) 106, 720, 1341
MspA1I CMGCKG 5 cut(s) 182, 257, 387, 860, 977
MspI CCGG 3 cut(s) 122, 503, 1348
MspR9I CCNGG 1 cut(s) 1349
NciI CCSGG 1 cut(s) 1349
NcoI CCATGG 1 cut(s) 1336
NdeII GATC 7 cut(s) 115, 313, 445, 598, 1273, 1352, 1532
NlaIII CATG 9 cut(s) 302, 353, 422, 695, 704, 719, 1229, 1340, 1577
NlaIV GGNNCC 3 cut(s) 249, 315, 564
NmeAIII GCCGAG 1 cut(s) 1364
PagI TCATGA 1 cut(s) 1225
PciSI GCTCTTC 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 1233
PflMI CCANNNNNTGG 1 cut(s) 206
PleI GAGTC 2 cut(s) 667, 1376
PpsI GAGTC 2 cut(s) 667, 1376
PsiI TTATAA 1 cut(s) 1310
PspFI CCCAGC 1 cut(s) 1415
PspN4I GGNNCC 3 cut(s) 249, 315, 564
PspPI GGNCC 1 cut(s) 368
PstI CTGCAG 5 cut(s) 880, 889, 985, 994, 1102
PstNI CAGNNNCTG 5 cut(s) 863, 884, 980, 989, 1253
PsuI RGATCY 2 cut(s) 313, 1532
PvuII CAGCTG 4 cut(s) 182, 257, 860, 977
RsaI GTAC 3 cut(s) 434, 729, 1499
RsaNI GTAC 3 cut(s) 433, 728, 1498
RseI CAYNNNNRTG 3 cut(s) 106, 720, 1341
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 1377
Sau3AI GATC 7 cut(s) 115, 313, 445, 598, 1273, 1352, 1532
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 2 cut(s) 668, 1376
ScrFI CCNGG 1 cut(s) 1349
SduI GDGCHC 4 cut(s) 59, 250, 442, 1543
SfaNI GCATC 3 cut(s) 98, 332, 779
SfcI CTRYAG 6 cut(s) 876, 885, 981, 990, 1098, 1551
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 3 cut(s) 106, 720, 1341
Sse9I AATT 6 cut(s) 170, 285, 336, 616, 1312, 1558
SsiI CCGC 5 cut(s) 347, 387, 401, 1118, 1409
SspMI CTAG 3 cut(s) 62, 1143, 1299
StyD4I CCNGG 1 cut(s) 1347
StyI CCWWGG 1 cut(s) 1336
TaaI ACNGT 4 cut(s) 378, 437, 465, 732
TaiI ACGT 4 cut(s) 431, 492, 1117, 1236
TaqI TCGA 2 cut(s) 5, 12
TasI AATT 6 cut(s) 170, 285, 336, 616, 1312, 1558
TatI WGTACW 2 cut(s) 432, 727
TauI GCSGC 2 cut(s) 403, 1121
Tru1I TTAA 1 cut(s) 1377
Tru9I TTAA 1 cut(s) 1377
TscAI CASTG 1 cut(s) 999
TspDTI ATGAA 6 cut(s) 315, 708, 753, 1203, 1214, 1242
TspRI CASTG 1 cut(s) 999
Van91I CCANNNNNTGG 1 cut(s) 206
VneI GTGCAC 1 cut(s) 438
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 4 cut(s) 170, 336, 616, 1312
XbaI TCTAGA 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 1 cut(s) 1334
XmiI GTMKAC 1 cut(s) 541
XmnI GAANNNNTTC 1 cut(s) 1233
XspI CTAG 3 cut(s) 62, 1143, 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.