MD00G1078400.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009241
Physical Location & Seq
Forward (+)
15606836 .. 15612271
5436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1078400.v1.1.491

Sequence Viewer

Length: 1617 bp
ATGTCGATGCTCGATTCCTTCTTCAACAAGGGCTTCAAAGCGGCCAAATGCAAAACCATGCTGAAATTGACAATTCCGCGGATAAAATTGTTGAGGAACAGGAGAGAAATTCAGATTAAACAGATGCGCCGCGACATTGCCAAGCTTCTTGAGACTGGCCAAGAGGCTACTGCAAGGATTCGGGTAGAACATATTATTAGAGAAGAGAACATGATGGCAGCTCAGGAGATCCTTGAGCTATTTTCTGAGCTTATTGCTGTCCGCCTTCCAATTATTGAATCACAAAGGGAATGCCCTATAGACTTGAAAGAAGCAATATCCAGTGTGTGTTTTGCTGCGCCAAGATGTGCTGATCTGCCGGAGTTGCTGCAAGTTCAAATGCTATTTGCTTCAAAATATGGGAAAGAATTTGTAGCAGCTGCAACAGAGCTCATACCGGATTGTGGTGTCAATCGCCAGTTGATAGAACTGCTATCTGTTCGTGCACCCTCACCTGAGAAAAAACTGAAGCTTCTGAAAGAAATTGCTGAAGAGCATGACCTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATTTACAAACCGCATGAAGATTTGTTGAGTGGTCCGACACAGTTTGTTAGTGGGTCTAAATTGCCCCTTCCTAAAGAAAAACAGGATGAAACATTGCAATACAGCCCAGATCAAGACCAGAAAGAACTGTCTGATTCTGATGACGGTTTGGACTCATTAGACTTTCCTGAAGTACCCAAGCAATCACTACGACCAAGTGCAAATCCAGCCTCAGCACCAGCAATGTCTCCTCCTTTACCAACCCATCCACACCCTGAAATTGATCATGACTTGTCAAAGAAATTTGGGTCCGTTGAAAGTCAATCTAAAGAGCCTTCCTTTGAGTCTGGAGAAGTGATGGAAGAAAGATCAGTAGCTAACAATCAGGAACAGTCTCATGTCTTGGTTGGTGCTGTGGAAGATAAGCAGTTTTTGCCATTTATTTCTTACCCTTCACTTTCTTCTGCGTCATTTTCAACGAGACAAAGCAATCCACCACCAAATCTTTCAAGAACAAGAAGTGAGGTCAACGTCGATTTGCAGGATGTGTTAGCTGCTGCTCAGGCTGCTGCGGACTCTGCAGAACGTGCAGCCACAGCGGCTCGCTCGGCAGCTAGTCTTGCACAGGTCAGAATCAACGAGATTACCAAGAAAAAAAGTGAAGTCGTCCCAGAAACTAGCTGTGAAAACCCATTTCATGTGGACGTTCCTAATCAGTCAGATACAAGAGAAAAACCAGACTCCAATCATCTTATCCCTGATGGTGGATCTGATGGTGTAATAGATTCTCCGGTACCCCATCCAATCCATTTATATGGCCAGGGATCAGAGATATCAAATGCTTCGTCTATCACTATGGAACCACTTAATGTTGATTTTGATTCCGCACATTCCAATGACCATGCTTCTGAGCATGAACCTGTTCGTCATCAGCCTCAGAGATTGGCTTCAATGGGGGATGACTCGTACTTCTCATACCCAAACTTATTTACATCGCAAAATTCAAATGTCGGATCTGATGCTCAGTCTTCTACAGATAATCCTTTCCCATCCCAAGGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

539

Amino Acids

59.34

Weight (kDa)

4.98

Isoelectric Point (pI)

59.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 6.4e-60 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1345
AccB1I GGYRCC 1 cut(s) 1345
AccII CGCG 2 cut(s) 79, 132
AciI CCGC 9 cut(s) 41, 77, 79, 130, 262, 584, 1124, 1151, 1437
AclWI GGATC 6 cut(s) 223, 545, 558, 1327, 1384, 1573
AcoI YGGCCR 3 cut(s) 42, 157, 1369
AcsI RAATTY 5 cut(s) 108, 407, 573, 854, 1552
AcuI CTGAAG 4 cut(s) 527, 549, 585, 762
AfaI GTAC 3 cut(s) 747, 1347, 1520
AfiI CCNNNNNNNGG 4 cut(s) 443, 547, 1316, 1607
AjnI CCWGG 2 cut(s) 540, 1371
Alw21I GWGCWC 2 cut(s) 432, 487
Alw26I GTCTC 4 cut(s) 146, 804, 951, 1027
Alw44I GTGCAC 1 cut(s) 483
AlwI GGATC 6 cut(s) 223, 545, 558, 1327, 1384, 1573
AoxI GGCC 3 cut(s) 42, 157, 1369
ApaLI GTGCAC 1 cut(s) 483
ApoI RAATTY 5 cut(s) 108, 407, 573, 854, 1552
ArsI GACNNNNNNTTYG 2 cut(s) 1382, 1414
Asp700I GAANNNNTTC 1 cut(s) 1473
Asp718I GGTACC 1 cut(s) 1345
AspLEI GCGC 2 cut(s) 129, 340
AspS9I GGNCC 2 cut(s) 605, 861
AsuHPI GGTGA 1 cut(s) 483
AvaII GGWCC 2 cut(s) 605, 861
BaeGI GKGCMC 2 cut(s) 487, 1614
BalI TGGCCA 2 cut(s) 159, 1371
BamHI GGATCC 1 cut(s) 550
BanI GGYRCC 1 cut(s) 1345
BanII GRGCYC 1 cut(s) 432
BbsI GAAGAC 1 cut(s) 1572
Bbv12I GWGCWC 2 cut(s) 432, 487
BbvCI CCTCAGC 1 cut(s) 784
BccI CCATC 7 cut(s) 208, 825, 904, 1307, 1319, 1359, 1609
BciT130I CCWGG 2 cut(s) 542, 1373
BclI TGATCA 1 cut(s) 835
BcoDI GTCTC 4 cut(s) 146, 804, 951, 1027
BfaI CTAG 2 cut(s) 1167, 1230
BfmI CTRYAG 3 cut(s) 297, 1131, 1584
BglI GCCNNNNNGGC 1 cut(s) 1151
Bme1390I CCNGG 2 cut(s) 542, 1373
Bme18I GGWCC 2 cut(s) 605, 861
BmgT120I GGNCC 2 cut(s) 605, 861
BmiI GGNNCC 4 cut(s) 552, 862, 1347, 1413
BmrFI CCNGG 2 cut(s) 542, 1373
BmsI GCATC 3 cut(s) 114, 569, 1561
BpiI GAAGAC 1 cut(s) 1572
BpmI CTGGAG 1 cut(s) 921
Bpu10I CCTNAGC 3 cut(s) 222, 784, 1113
BpuEI CTTGAG 2 cut(s) 170, 254
BsaBI GATNNNNATC 1 cut(s) 549
BsaJI CCNNGG 3 cut(s) 77, 1372, 1606
BsaWI WCCGGW 2 cut(s) 436, 1342
Bsc4I CCNNNNNNNGG 4 cut(s) 443, 547, 1316, 1607
Bse1I ACTGG 3 cut(s) 160, 321, 457
Bse3DI GCAATG 3 cut(s) 135, 665, 801
Bse8I GATNNNNATC 1 cut(s) 549
BseBI CCWGG 2 cut(s) 542, 1373
BseDI CCNNGG 3 cut(s) 77, 1372, 1606
BseGI GGATG 6 cut(s) 664, 817, 1102, 1351, 1516, 1601
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 4 cut(s) 443, 547, 1316, 1607
BseMI GCAATG 3 cut(s) 135, 665, 801
BseMII CTCAG 8 cut(s) 236, 237, 486, 798, 1127, 1452, 1502, 1589
BseNI ACTGG 3 cut(s) 160, 321, 457
BseRI GAGGAG 1 cut(s) 792
BseSI GKGCMC 2 cut(s) 487, 1614
BsgI GTGCAG 1 cut(s) 1161
Bsh1236I CGCG 2 cut(s) 79, 132
BshFI GGCC 3 cut(s) 44, 159, 1371
BshNI GGYRCC 1 cut(s) 1345
BsiHKAI GWGCWC 2 cut(s) 432, 487
BsiSI CCGG 3 cut(s) 359, 437, 1343
BslFI GGGAC 1 cut(s) 1205
BslI CCNNNNNNNGG 4 cut(s) 443, 547, 1316, 1607
BsmAI GTCTC 4 cut(s) 146, 804, 951, 1027
BsmFI GGGAC 1 cut(s) 1205
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 3 cut(s) 44, 159, 1371
Bsp1286I GDGCHC 3 cut(s) 432, 487, 1614
Bsp143I GATC 9 cut(s) 228, 352, 550, 682, 835, 920, 1319, 1376, 1565
BspACI CCGC 9 cut(s) 41, 77, 79, 130, 262, 584, 1124, 1151, 1437
BspANI GGCC 3 cut(s) 44, 159, 1371
BspCNI CTCAG 8 cut(s) 235, 238, 487, 797, 1126, 1453, 1501, 1588
BspFNI CGCG 2 cut(s) 79, 132
BspHI TCATGA 1 cut(s) 838
BspLI GGNNCC 4 cut(s) 552, 862, 1347, 1413
BspMAI CTGCAG 1 cut(s) 1135
BspPI GGATC 6 cut(s) 223, 545, 558, 1327, 1384, 1573
BspQI GCTCTTC 1 cut(s) 525
BspT107I GGYRCC 1 cut(s) 1345
BsrDI GCAATG 3 cut(s) 135, 665, 801
BsrI ACTGG 3 cut(s) 160, 321, 457
BssECI CCNNGG 3 cut(s) 77, 1372, 1606
BssMI GATC 9 cut(s) 228, 352, 550, 682, 835, 920, 1319, 1376, 1565
BssT1I CCWWGG 1 cut(s) 1606
Bst2UI CCWGG 2 cut(s) 542, 1373
Bst4CI ACNGT 4 cut(s) 615, 702, 719, 945
Bst6I CTCTTC 2 cut(s) 198, 525
BstAPI GCANNNNNTGC 2 cut(s) 985, 1139
BstC8I GCNNGC 1 cut(s) 1156
BstDEI CTNAG 8 cut(s) 222, 246, 495, 784, 1113, 1461, 1488, 1575
BstDSI CCRYGG 1 cut(s) 77
BstF5I GGATG 6 cut(s) 664, 817, 1102, 1351, 1516, 1601
BstFNI CGCG 2 cut(s) 79, 132
BstHHI GCGC 2 cut(s) 129, 340
BstKTI GATC 9 cut(s) 231, 355, 553, 685, 838, 923, 1322, 1379, 1568
BstMAI GTCTC 4 cut(s) 146, 804, 951, 1027
BstMBI GATC 9 cut(s) 228, 352, 550, 682, 835, 920, 1319, 1376, 1565
BstNI CCWGG 2 cut(s) 542, 1373
BstSCI CCNGG 2 cut(s) 540, 1371
BstSFI CTRYAG 3 cut(s) 297, 1131, 1584
BstSLI GKGCMC 2 cut(s) 487, 1614
BstUI CGCG 2 cut(s) 79, 132
BstV2I GAAGAC 1 cut(s) 1572
BstX2I RGATCY 4 cut(s) 228, 550, 1319, 1565
BstXI CCANNNNNNTGG 1 cut(s) 1367
BstYI RGATCY 4 cut(s) 228, 550, 1319, 1565
BsuRI GGCC 3 cut(s) 44, 159, 1371
BtgI CCRYGG 1 cut(s) 77
BtgZI GCGATG 1 cut(s) 1530
BtsCI GGATG 6 cut(s) 664, 817, 1102, 1351, 1516, 1601
BtsIMutI CAGTG 2 cut(s) 328, 1612
Cac8I GCNNGC 1 cut(s) 1156
CciI TCATGA 1 cut(s) 838
CfoI GCGC 2 cut(s) 129, 340
Cfr13I GGNCC 2 cut(s) 605, 861
Cfr42I CCGCGG 1 cut(s) 80
CseI GACGC 1 cut(s) 1008
Csp6I GTAC 3 cut(s) 746, 1346, 1519
CviAII CATG 9 cut(s) 58, 211, 536, 587, 839, 950, 1250, 1454, 1466
CviQI GTAC 3 cut(s) 746, 1346, 1519
DdeI CTNAG 8 cut(s) 222, 246, 495, 784, 1113, 1461, 1488, 1575
DpnI GATC 9 cut(s) 230, 354, 552, 684, 837, 922, 1321, 1378, 1567
DpnII GATC 9 cut(s) 228, 352, 550, 682, 835, 920, 1319, 1376, 1565
EaeI YGGCCR 3 cut(s) 42, 157, 1369
Eam1104I CTCTTC 2 cut(s) 198, 525
EarI CTCTTC 2 cut(s) 198, 525
EciI GGCGGA 1 cut(s) 251
Ecl136II GAGCTC 1 cut(s) 430
Eco130I CCWWGG 1 cut(s) 1606
Eco24I GRGCYC 1 cut(s) 432
Eco32I GATATC 1 cut(s) 1386
Eco47I GGWCC 2 cut(s) 605, 861
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 4 cut(s) 527, 549, 585, 762
EcoICRI GAGCTC 1 cut(s) 430
EcoRII CCWGG 2 cut(s) 540, 1371
EcoRV GATATC 1 cut(s) 1386
EcoT14I CCWWGG 1 cut(s) 1606
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 1606
FaeI CATG 9 cut(s) 61, 214, 539, 590, 842, 953, 1253, 1457, 1469
FaqI GGGAC 1 cut(s) 1205
FatI CATG 9 cut(s) 57, 210, 535, 586, 838, 949, 1249, 1453, 1465
FbaI TGATCA 1 cut(s) 835
FokI GGATG 6 cut(s) 671, 804, 1109, 1338, 1523, 1588
FriOI GRGCYC 1 cut(s) 432
FspBI CTAG 2 cut(s) 1167, 1230
GlaI GCGC 2 cut(s) 128, 339
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 3 cut(s) 44, 159, 1371
HapII CCGG 3 cut(s) 359, 437, 1343
HgaI GACGC 1 cut(s) 1008
HhaI GCGC 2 cut(s) 129, 340
Hin1II CATG 9 cut(s) 61, 214, 539, 590, 842, 953, 1253, 1457, 1469
Hin6I GCGC 2 cut(s) 127, 338
HinP1I GCGC 2 cut(s) 127, 338
HincII GTYRAC 1 cut(s) 1081
HindII GTYRAC 1 cut(s) 1081
HindIII AAGCTT 2 cut(s) 143, 509
HpaII CCGG 3 cut(s) 359, 437, 1343
HphI GGTGA 1 cut(s) 483
Hpy166II GTNNAC 3 cut(s) 485, 1081, 1255
Hpy188III TCNNGA 8 cut(s) 149, 224, 686, 740, 839, 900, 938, 1062
Hpy8I GTNNAC 3 cut(s) 485, 1081, 1255
Hpy99I CGWCG 1 cut(s) 1088
HpyAV CCTTC 5 cut(s) 28, 275, 650, 897, 1014
HpyCH4III ACNGT 4 cut(s) 615, 702, 719, 945
HpyCH4IV ACGT 3 cut(s) 1083, 1138, 1257
HpyF3I CTNAG 8 cut(s) 222, 246, 495, 784, 1113, 1461, 1488, 1575
HpySE526I ACGT 3 cut(s) 1083, 1138, 1257
Hsp92II CATG 9 cut(s) 61, 214, 539, 590, 842, 953, 1253, 1457, 1469
HspAI GCGC 2 cut(s) 127, 338
KpnI GGTACC 1 cut(s) 1349
Ksp22I TGATCA 1 cut(s) 835
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 9 cut(s) 228, 352, 550, 682, 835, 920, 1319, 1376, 1565
LguI GCTCTTC 1 cut(s) 525
LweI GCATC 3 cut(s) 114, 569, 1561
MaeI CTAG 2 cut(s) 1167, 1230
MaeII ACGT 3 cut(s) 1083, 1138, 1257
MalI GATC 9 cut(s) 230, 354, 552, 684, 837, 922, 1321, 1378, 1567
MboI GATC 9 cut(s) 228, 352, 550, 682, 835, 920, 1319, 1376, 1565
MboII GAAGA 8 cut(s) 13, 215, 542, 602, 926, 983, 1005, 1572
MflI RGATCY 4 cut(s) 228, 550, 1319, 1565
MhlI GDGCHC 3 cut(s) 432, 487, 1614
MlsI TGGCCA 2 cut(s) 159, 1371
MluNI TGGCCA 2 cut(s) 159, 1371
MlyI GAGTC 5 cut(s) 719, 905, 1121, 1286, 1508
MmeI TCCRAC 2 cut(s) 632, 1543
MnlI CCTC 7 cut(s) 87, 157, 499, 793, 813, 1069, 1497
Mox20I TGGCCA 2 cut(s) 159, 1371
MroXI GAANNNNTTC 1 cut(s) 1473
MscI TGGCCA 2 cut(s) 159, 1371
MseI TTAA 2 cut(s) 117, 1419
MslI CAYNNNNRTG 2 cut(s) 1365, 1446
Msp20I TGGCCA 2 cut(s) 159, 1371
MspA1I CMGCKG 3 cut(s) 79, 419, 1151
MspI CCGG 3 cut(s) 359, 437, 1343
MspR9I CCNGG 2 cut(s) 542, 1373
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 2 cut(s) 542, 1373
MvnI CGCG 2 cut(s) 79, 132
NdeII GATC 9 cut(s) 228, 352, 550, 682, 835, 920, 1319, 1376, 1565
NlaIII CATG 9 cut(s) 61, 214, 539, 590, 842, 953, 1253, 1457, 1469
NlaIV GGNNCC 4 cut(s) 552, 862, 1347, 1413
NmeAIII GCCGAG 1 cut(s) 1139
PagI TCATGA 1 cut(s) 838
PciSI GCTCTTC 1 cut(s) 525
PctI GAATGC 1 cut(s) 296
PdmI GAANNNNTTC 1 cut(s) 1473
PfeI GAWTC 7 cut(s) 14, 178, 278, 707, 1185, 1337, 1433
PleI GAGTC 5 cut(s) 719, 904, 1121, 1286, 1508
PpsI GAGTC 5 cut(s) 719, 904, 1121, 1286, 1508
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 540, 1371
PspGI CCWGG 2 cut(s) 540, 1371
PspN4I GGNNCC 4 cut(s) 552, 862, 1347, 1413
PspPI GGNCC 2 cut(s) 605, 861
PstI CTGCAG 1 cut(s) 1135
PsuI RGATCY 4 cut(s) 228, 550, 1319, 1565
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 3 cut(s) 747, 1347, 1520
RsaNI GTAC 3 cut(s) 746, 1346, 1519
RseI CAYNNNNRTG 2 cut(s) 1365, 1446
SacI GAGCTC 1 cut(s) 432
SacII CCGCGG 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 2 cut(s) 117, 1419
Sau3AI GATC 9 cut(s) 228, 352, 550, 682, 835, 920, 1319, 1376, 1565
Sau96I GGNCC 2 cut(s) 605, 861
SchI GAGTC 5 cut(s) 719, 905, 1121, 1286, 1508
ScrFI CCNGG 2 cut(s) 542, 1373
SduI GDGCHC 3 cut(s) 432, 487, 1614
SfaNI GCATC 3 cut(s) 114, 569, 1561
SfcI CTRYAG 3 cut(s) 297, 1131, 1584
Sfr303I CCGCGG 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 2 cut(s) 605, 861
SmiMI CAYNNNNRTG 2 cut(s) 1365, 1446
SmlI CTYRAG 2 cut(s) 149, 233
SmoI CTYRAG 2 cut(s) 149, 233
SsiI CCGC 9 cut(s) 41, 77, 79, 130, 262, 584, 1124, 1151, 1437
SspMI CTAG 2 cut(s) 1167, 1230
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 2 cut(s) 540, 1371
StyI CCWWGG 1 cut(s) 1606
TaaI ACNGT 4 cut(s) 615, 702, 719, 945
TaiI ACGT 3 cut(s) 1086, 1141, 1260
TaqI TCGA 3 cut(s) 5, 12, 1086
TauI GCSGC 3 cut(s) 44, 132, 1154
TfiI GAWTC 7 cut(s) 14, 178, 278, 707, 1185, 1337, 1433
Tru1I TTAA 2 cut(s) 117, 1419
Tru9I TTAA 2 cut(s) 117, 1419
TscAI CASTG 1 cut(s) 328
TspDTI ATGAA 4 cut(s) 603, 675, 1238, 1482
TspGWI ACGGA 1 cut(s) 853
TspRI CASTG 1 cut(s) 328
VneI GTGCAC 1 cut(s) 483
VpaK11BI GGWCC 2 cut(s) 605, 861
XapI RAATTY 5 cut(s) 108, 407, 573, 854, 1552
XmnI GAANNNNTTC 1 cut(s) 1473
XspI CTAG 2 cut(s) 1167, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.