RLG00000013655

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009241
Physical Location & Seq
Reverse (-)
35162415 .. 35167696
5282 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013655

Sequence Viewer

Length: 1812 bp
ATGTCGATGCTCGATTCCTTCTTCAACAAGGGCTTCAAAGCTGCCAAATGCAAAACTCTGCTGAAATTGACGATTCCGCGGATAAAGTTGTTGAGGAACAGGAGAGAAATTCAGATTAAACAGATGCGCCGTGACATTGCCAAGCTTCTTCAGACTGGCCAGGAAGCTACAGCCCGGATTCGGGTGGAGCATATTATTAGAGAAGAGAATATGATGGCAGCTCAGGAGATCATCGAGCTATATGCTGAGCTTATTTCTGTCCGCCTTCCAATAGTTGAATCACAAAGGGAGTGCCCTCTAGACTTGAAAGAAGCAATATCCAGTTTGTGTTTTGCTGCACCAAGATGCGCTGATCTGCCGGAGTTGCTTCAAGTTCAAATGCTATTTGTATCTAAATATGGGAAAGAATTTGTATCAGCTGCAACAGAGCTAATGCCAGATTGTGGTGTCAATCGCCAGTTGATAGAGCTGCTATCTGTTCGGGCTCCTTCAGCTGAGAAAAAGCTGAAGCTTTTGAAAGAAATTGCTGAAGAGCATGAACTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATTTTCAAACCGCATGAGGATTTGCTGAATGGTCCGACACAGTTTGTCAGTGGGTCTAAGTTGCCGCTTCCCGATGAAAAACATGATGAGACATTGTACTGTGCTCCTGATCAAGCTCAGAAAGAACAGTCTGATTCTGATGATGGTTTGGACGTTTTAGACTTTCCGGAAGTTCCAAGGGTATCACCAACTGAGAGTATACCCTCAGCACCAGCGATGGCTCCACCTTTTCCACCCCCACCACACCCTGATTTTGATCACGAGCTATCAAAAAATTCTGAGGCGATTGGACATCCATCCAAAGAGCCGTCCTTTGAGTCTGGAGAGGAAAGAGCAATGGCAGACAAGCATGAATCTCATGCTTCAGTTGGTCATGGGGATGTACAGTTTTTGCCATTTATATCTCCCCCATCTCTTTCGTCTGCATCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTCAGCTGCTGCTCAGGCTGCTGCAGATTCTGCAGCGTTTCCTGTGAGACAAACGAATCCACCACCAACCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTCAGCTGCTGCAGATTCTGCAGCGTTTCCTGTGAGACAAACAAATCCACCACCACCCTCTCCTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTTGGCTGCTGCTCAGGCTGCTGCAGATTCGGCAGAACGTGCGGCAGCAGCAGCTCGCTCAGCTGCTAGTCTTGCACAGGTAAGAATAAATGAGCTGACCAAGAAAAACAATGAAGAAGTATCTGAAAGTAGCTGTGAAAACCCATTTCATGAAGACGTTCCTAATCAGTCAGCTACTAAAGAAAAGTTGGATTTTGATCATCATATCCCTGATGGTGATTCTAGTGGTGTTATAAATTCTACAGAACCCCATCGAGGCCATGAATATGGCCGGGGATCAGAGACATCAAATCTTTCGTTTAAGGACTCGGCTAAAGTGGATTTTGATTCCCCTCTTCCCAGCGATAATGTTTTTGAACACGAGCCAGCTCATGACCAGCCTCAGAGATTGACTTCAATGGATGATGACCCATACTTCTCATACCCAAACTTATTCACATCAGAAAACAATGTTGGATCTGGTGCTCAGTCGTCTACAGATAATTCTCGCTTCTCGCATGAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

604

Amino Acids

66.35

Weight (kDa)

5.01

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 1.3e-59 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1538
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 2 cut(s) 772, 1778
AccII CGCG 1 cut(s) 79
AccIII TCCGGA 1 cut(s) 739
AciI CCGC 6 cut(s) 77, 79, 262, 584, 638, 1346
AclWI GGATC 4 cut(s) 545, 558, 1588, 1768
AcoI YGGCCR 2 cut(s) 157, 1573
AcsI RAATTY 5 cut(s) 108, 407, 573, 847, 1540
AcuI CTGAAG 6 cut(s) 134, 474, 527, 549, 585, 921
AfaI GTAC 2 cut(s) 671, 957
AfiI CCNNNNNNNGG 4 cut(s) 180, 181, 443, 1559
AjnI CCWGG 1 cut(s) 159
AjuI GAANNNNNNNTTGG 4 cut(s) 1141, 1173, 1740, 1772
Alw21I GWGCWC 2 cut(s) 679, 1771
Alw26I GTCTC 5 cut(s) 656, 1006, 1123, 1228, 1580
AlwI GGATC 4 cut(s) 545, 558, 1588, 1768
AlwNI CAGNNNCTG 4 cut(s) 1091, 1112, 1208, 1217
Aor13HI TCCGGA 1 cut(s) 739
AoxI GGCC 3 cut(s) 157, 1561, 1573
ApoI RAATTY 5 cut(s) 108, 407, 573, 847, 1540
Asp700I GAANNNNTTC 1 cut(s) 1461
AspLEI GCGC 2 cut(s) 129, 350
AspS9I GGNCC 1 cut(s) 605
AsuC2I CCSGG 2 cut(s) 175, 1577
AsuHPI GGTGA 2 cut(s) 750, 1532
AvaII GGWCC 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 296
BalI TGGCCA 1 cut(s) 159
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 487
BauI CACGAG 2 cut(s) 833, 1664
BbsI GAAGAC 1 cut(s) 1464
Bbv12I GWGCWC 2 cut(s) 679, 1771
BbvCI CCTCAGC 1 cut(s) 778
BccI CCATC 7 cut(s) 208, 710, 784, 877, 991, 1511, 1563
BceAI ACGGC 2 cut(s) 114, 865
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 1 cut(s) 161
BclI TGATCA 3 cut(s) 682, 829, 1501
BcnI CCSGG 2 cut(s) 175, 1577
BcoDI GTCTC 5 cut(s) 656, 1006, 1123, 1228, 1580
BfaI CTAG 3 cut(s) 299, 1371, 1527
BfmI CTRYAG 8 cut(s) 168, 1104, 1113, 1209, 1218, 1326, 1545, 1779
BlpI GCTNAGC 2 cut(s) 246, 1363
Bme1390I CCNGG 3 cut(s) 161, 175, 1577
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 3 cut(s) 486, 552, 795
BmrFI CCNGG 3 cut(s) 161, 175, 1577
BmsI GCATC 4 cut(s) 114, 335, 569, 1007
BpiI GAAGAC 1 cut(s) 1464
BpmI CTGGAG 1 cut(s) 915
Bpu10I CCTNAGC 4 cut(s) 222, 778, 1095, 1317
Bpu1102I GCTNAGC 2 cut(s) 246, 1363
BpuMI CCSGG 2 cut(s) 175, 1577
BsaBI GATNNNNATC 3 cut(s) 549, 828, 1500
BsaJI CCNNGG 3 cut(s) 77, 749, 1576
BsaWI WCCGGW 1 cut(s) 739
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 181, 443, 1559
Bse1I ACTGG 4 cut(s) 160, 321, 457, 546
Bse3DI GCAATG 2 cut(s) 135, 915
Bse8I GATNNNNATC 3 cut(s) 549, 828, 1500
BseAI TCCGGA 1 cut(s) 739
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 3 cut(s) 77, 749, 1576
BseGI GGATG 4 cut(s) 865, 869, 958, 1711
BseJI GATNNNNATC 3 cut(s) 549, 828, 1500
BseLI CCNNNNNNNGG 4 cut(s) 180, 181, 443, 1559
BseMI GCAATG 2 cut(s) 135, 915
BseNI ACTGG 4 cut(s) 160, 321, 457, 546
BseSI GKGCMC 1 cut(s) 296
BseYI CCCAGC 1 cut(s) 1643
BsgI GTGCAG 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 79
BshFI GGCC 3 cut(s) 159, 1563, 1575
BsiHKAI GWGCWC 2 cut(s) 679, 1771
BsiSI CCGG 4 cut(s) 175, 359, 740, 1576
BslI CCNNNNNNNGG 4 cut(s) 180, 181, 443, 1559
BsmAI GTCTC 5 cut(s) 656, 1006, 1123, 1228, 1580
BsnI GGCC 3 cut(s) 159, 1563, 1575
Bsp1286I GDGCHC 4 cut(s) 296, 487, 679, 1771
Bsp13I TCCGGA 1 cut(s) 739
Bsp1407I TGTACA 1 cut(s) 955
Bsp143I GATC 8 cut(s) 228, 352, 550, 682, 829, 1501, 1580, 1760
Bsp1720I GCTNAGC 2 cut(s) 246, 1363
BspACI CCGC 6 cut(s) 77, 79, 262, 584, 638, 1346
BspANI GGCC 3 cut(s) 159, 1563, 1575
BspEI TCCGGA 1 cut(s) 739
BspFNI CGCG 1 cut(s) 79
BspHI TCATGA 2 cut(s) 1453, 1675
BspLI GGNNCC 3 cut(s) 486, 552, 795
BspMAI CTGCAG 5 cut(s) 1108, 1117, 1213, 1222, 1330
BspPI GGATC 4 cut(s) 545, 558, 1588, 1768
BspQI GCTCTTC 1 cut(s) 525
BsrDI GCAATG 2 cut(s) 135, 915
BsrGI TGTACA 1 cut(s) 955
BsrI ACTGG 4 cut(s) 160, 321, 457, 546
BssECI CCNNGG 3 cut(s) 77, 749, 1576
BssMI GATC 8 cut(s) 228, 352, 550, 682, 829, 1501, 1580, 1760
BssNAI GTATAC 1 cut(s) 773
BssSI CACGAG 2 cut(s) 833, 1664
BssT1I CCWWGG 1 cut(s) 749
Bst1107I GTATAC 1 cut(s) 773
Bst2BI CACGAG 2 cut(s) 833, 1664
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 4 cut(s) 615, 674, 702, 960
Bst6I CTCTTC 3 cut(s) 198, 525, 1644
BstAPI GCANNNNNTGC 3 cut(s) 1112, 1217, 1343
BstAUI TGTACA 1 cut(s) 955
BstC8I GCNNGC 2 cut(s) 1360, 1671
BstDSI CCRYGG 1 cut(s) 77
BstF5I GGATG 4 cut(s) 865, 869, 958, 1711
BstFNI CGCG 1 cut(s) 79
BstHHI GCGC 2 cut(s) 129, 350
BstKTI GATC 8 cut(s) 231, 355, 553, 685, 832, 1504, 1583, 1763
BstMAI GTCTC 5 cut(s) 656, 1006, 1123, 1228, 1580
BstMBI GATC 8 cut(s) 228, 352, 550, 682, 829, 1501, 1580, 1760
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 3 cut(s) 159, 173, 1575
BstSFI CTRYAG 8 cut(s) 168, 1104, 1113, 1209, 1218, 1326, 1545, 1779
BstSLI GKGCMC 1 cut(s) 296
BstUI CGCG 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 1464
BstX2I RGATCY 2 cut(s) 550, 1760
BstXI CCANNNNNNTGG 1 cut(s) 1571
BstYI RGATCY 2 cut(s) 550, 1760
BstZ17I GTATAC 1 cut(s) 773
BsuRI GGCC 3 cut(s) 159, 1563, 1575
BtgI CCRYGG 1 cut(s) 77
BtgZI GCGATG 1 cut(s) 803
BtsCI GGATG 4 cut(s) 865, 869, 958, 1711
BtsIMutI CAGTG 2 cut(s) 628, 1807
Cac8I GCNNGC 2 cut(s) 1360, 1671
CaiI CAGNNNCTG 4 cut(s) 1091, 1112, 1208, 1217
CciI TCATGA 2 cut(s) 1453, 1675
CfoI GCGC 2 cut(s) 129, 350
Cfr13I GGNCC 1 cut(s) 605
Cfr42I CCGCGG 1 cut(s) 80
Csp6I GTAC 2 cut(s) 670, 956
CviQI GTAC 2 cut(s) 670, 956
DpnI GATC 8 cut(s) 230, 354, 552, 684, 831, 1503, 1582, 1762
DpnII GATC 8 cut(s) 228, 352, 550, 682, 829, 1501, 1580, 1760
EaeI YGGCCR 2 cut(s) 157, 1573
Eam1104I CTCTTC 3 cut(s) 198, 525, 1644
EarI CTCTTC 3 cut(s) 198, 525, 1644
EciI GGCGGA 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 749
Eco24I GRGCYC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 605
Eco57I CTGAAG 6 cut(s) 134, 474, 527, 549, 585, 921
EcoRII CCWGG 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 749
EcoT38I GRGCYC 1 cut(s) 487
ErhI CCWWGG 1 cut(s) 749
FbaI TGATCA 3 cut(s) 682, 829, 1501
FblI GTMKAC 2 cut(s) 772, 1778
FokI GGATG 4 cut(s) 852, 856, 965, 1718
FriOI GRGCYC 1 cut(s) 487
FspBI CTAG 3 cut(s) 299, 1371, 1527
GlaI GCGC 2 cut(s) 128, 349
GsaI CCCAGC 1 cut(s) 1647
GsuI CTGGAG 1 cut(s) 915
HaeIII GGCC 3 cut(s) 159, 1563, 1575
HapII CCGG 4 cut(s) 175, 359, 740, 1576
HhaI GCGC 2 cut(s) 129, 350
Hin6I GCGC 2 cut(s) 127, 348
HinP1I GCGC 2 cut(s) 127, 348
HindIII AAGCTT 2 cut(s) 143, 509
HpaII CCGG 4 cut(s) 175, 359, 740, 1576
HphI GGTGA 2 cut(s) 750, 1532
Hpy166II GTNNAC 2 cut(s) 773, 1779
Hpy8I GTNNAC 2 cut(s) 773, 1779
HpyAV CCTTC 4 cut(s) 28, 275, 498, 1272
HpyCH4III ACNGT 4 cut(s) 615, 674, 702, 960
HpyCH4IV ACGT 3 cut(s) 726, 1342, 1461
HpySE526I ACGT 3 cut(s) 726, 1342, 1461
HspAI GCGC 2 cut(s) 127, 348
Kpn2I TCCGGA 1 cut(s) 739
Ksp22I TGATCA 3 cut(s) 682, 829, 1501
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 8 cut(s) 228, 352, 550, 682, 829, 1501, 1580, 1760
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 4 cut(s) 187, 490, 682, 799
LweI GCATC 4 cut(s) 114, 335, 569, 1007
MaeI CTAG 3 cut(s) 299, 1371, 1527
MaeII ACGT 3 cut(s) 726, 1342, 1461
MaeIII GTNAC 1 cut(s) 131
MalI GATC 8 cut(s) 230, 354, 552, 684, 831, 1503, 1582, 1762
MboI GATC 8 cut(s) 228, 352, 550, 682, 829, 1501, 1580, 1760
MflI RGATCY 2 cut(s) 550, 1760
MhlI GDGCHC 4 cut(s) 296, 487, 679, 1771
MlsI TGGCCA 1 cut(s) 159
MluCI AATT 8 cut(s) 65, 108, 407, 522, 573, 847, 1540, 1786
MluNI TGGCCA 1 cut(s) 159
MlyI GAGTC 2 cut(s) 899, 1604
MmeI TCCRAC 3 cut(s) 632, 1473, 1738
Mox20I TGGCCA 1 cut(s) 159
MroI TCCGGA 1 cut(s) 739
MroXI GAANNNNTTC 1 cut(s) 1461
MscI TGGCCA 1 cut(s) 159
MseI TTAA 2 cut(s) 117, 1605
MslI CAYNNNNRTG 3 cut(s) 343, 951, 1569
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 6 cut(s) 79, 419, 494, 1088, 1205, 1367
MspI CCGG 4 cut(s) 175, 359, 740, 1576
MspR9I CCNGG 3 cut(s) 161, 175, 1577
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 1 cut(s) 79
NciI CCSGG 2 cut(s) 175, 1577
NdeII GATC 8 cut(s) 228, 352, 550, 682, 829, 1501, 1580, 1760
NlaIV GGNNCC 3 cut(s) 486, 552, 795
NmeAIII GCCGAG 1 cut(s) 1592
NmuCI GTSAC 1 cut(s) 131
PagI TCATGA 2 cut(s) 1453, 1675
PciSI GCTCTTC 1 cut(s) 525
PcsI WCGNNNNNNNCGW 1 cut(s) 998
PdmI GAANNNNTTC 1 cut(s) 1461
PflMI CCANNNNNTGG 1 cut(s) 443
PleI GAGTC 2 cut(s) 898, 1604
PpsI GAGTC 2 cut(s) 898, 1604
PsiI TTATAA 1 cut(s) 1538
Psp6I CCWGG 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 1643
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 3 cut(s) 486, 552, 795
PspPI GGNCC 1 cut(s) 605
PstI CTGCAG 5 cut(s) 1108, 1117, 1213, 1222, 1330
PstNI CAGNNNCTG 4 cut(s) 1091, 1112, 1208, 1217
PsuI RGATCY 2 cut(s) 550, 1760
PvuII CAGCTG 5 cut(s) 419, 494, 1088, 1205, 1367
RsaI GTAC 2 cut(s) 671, 957
RsaNI GTAC 2 cut(s) 670, 956
RseI CAYNNNNRTG 3 cut(s) 343, 951, 1569
SacII CCGCGG 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 2 cut(s) 117, 1605
Sau3AI GATC 8 cut(s) 228, 352, 550, 682, 829, 1501, 1580, 1760
Sau96I GGNCC 1 cut(s) 605
SchI GAGTC 2 cut(s) 899, 1604
ScrFI CCNGG 3 cut(s) 161, 175, 1577
SduI GDGCHC 4 cut(s) 296, 487, 679, 1771
SfaNI GCATC 4 cut(s) 114, 335, 569, 1007
SfcI CTRYAG 8 cut(s) 168, 1104, 1113, 1209, 1218, 1326, 1545, 1779
Sfr303I CCGCGG 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 3 cut(s) 343, 951, 1569
Sse9I AATT 8 cut(s) 65, 108, 407, 522, 573, 847, 1540, 1786
SsiI CCGC 6 cut(s) 77, 79, 262, 584, 638, 1346
SspMI CTAG 3 cut(s) 299, 1371, 1527
StyD4I CCNGG 3 cut(s) 159, 173, 1575
StyI CCWWGG 1 cut(s) 749
TaaI ACNGT 4 cut(s) 615, 674, 702, 960
TaiI ACGT 3 cut(s) 729, 1345, 1464
TaqI TCGA 4 cut(s) 5, 12, 234, 1558
TasI AATT 8 cut(s) 65, 108, 407, 522, 573, 847, 1540, 1786
TatI WGTACW 2 cut(s) 669, 955
TauI GCSGC 2 cut(s) 640, 1349
Tru1I TTAA 2 cut(s) 117, 1605
Tru9I TTAA 2 cut(s) 117, 1605
TscAI CASTG 1 cut(s) 628
TseFI GTSAC 1 cut(s) 131
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 7 cut(s) 552, 663, 939, 1431, 1442, 1470, 1581
TspRI CASTG 1 cut(s) 628
Van91I CCANNNNNTGG 1 cut(s) 443
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 5 cut(s) 108, 407, 573, 847, 1540
XbaI TCTAGA 1 cut(s) 298
XmiI GTMKAC 2 cut(s) 772, 1778
XmnI GAANNNNTTC 1 cut(s) 1461
XspI CTAG 3 cut(s) 299, 1371, 1527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.