AT1G45976

BOI-related E3 ubiquitin-protein ligase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010150
Physical Location & Seq
Forward (+)
17199314 .. 17200841
1528 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G45976.1

Sequence Viewer

Length: 978 bp
ATGGCTCTTCATCCACACCTTCAAGACTCCAAGAATTTCAGAGACTTTTGCGGAATCGACGGCCAGATTTCGCCGGAATTAGGATTTAACCGCTCAGAGAATCTCCATGATCAATCTCAGCATCCTCCATATATTCCTCCATTTCATGTTGCTGGTTTTGCACCGGGACCGGTGGTTCAAATTGATGGTTCTGATGGTGGTAATGGAGCTGACTTTGAGTGGAACTATGGTCTTGGTCTTGAACCAAGACGTGAGAGGATAAAGGAACAAGATTTCTTGGAAAACAATTCTCAGATATCTTCTATTGATTTTTTGCAAGCTCGGTCTGTATCAACCGGATTGGGTCTGTCTCTTGACAATGCTCGTGTCGCTTCTTCTGATGGCTCTGCGTTGTTGTCGCTTGTTGGAGACGATATTGATCGCGAGTTACAGCGGCAAGATGCAGATATCGATAGGTTTCTCAAGATTCAGGGGGATCAGTTACGGCATGCTATCCTCGACAAGATCAAGAGGGGTCAACAGAAAACGGTGTCACTAATGGAGGAAAAAGTTGTTCAGAAACTCCGTGAGAAAGATGAGGAGCTAGAGAGGATAAACCGAAAGAACAAGGAACTCGAGGTGCGAATGGAACAGCTGACAATGGAAGCTGAGGCATGGCAGCAACGTGCAAAGTACAACGAGAATATGATTGCTGCACTCAACTACAATCTTGACCGAGCTCAAGGTCGGCCAAGAGATAGCATTGAAGGATGTGGAGACAGTGAAGTAGATGACACAGCTTCTTGCTTCAATGGGAGAGACAACAGCAACAATAACACGAAGACGATGATGATGTGCAGGTTTTGTGGAGTGAGAGAAATGTGTATGTTGTTGTTGCCATGTAATCATATGTGTTTGTGTAAGGAGTGTGAGAGGAAGCTTAGCTCTTGTCCTTTGTGTCAGTCTTCTAAGTTTCTAGGTATGGAAGTATACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

325

Amino Acids

36.86

Weight (kDa)

5.17

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 277 - 315 8.9e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 828
AccBSI CCGCTC 1 cut(s) 93
AccI GTMKAC 1 cut(s) 969
AccII CGCG 1 cut(s) 423
AciI CCGC 3 cut(s) 51, 91, 433
AclWI GGATC 1 cut(s) 483
AcoI YGGCCR 2 cut(s) 61, 728
AcsI RAATTY 1 cut(s) 34
AfaI GTAC 1 cut(s) 674
AfiI CCNNNNNNNGG 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 972
AgeI ACCGGT 1 cut(s) 169
AgsI TTSAA 5 cut(s) 23, 179, 242, 746, 790
AjiI CACGTC 1 cut(s) 251
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
AluBI AGCT 9 cut(s) 209, 320, 583, 634, 647, 719, 779, 919, 924
AluI AGCT 9 cut(s) 209, 320, 583, 634, 647, 719, 779, 919, 924
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 5 cut(s) 36, 354, 402, 750, 792
AlwI GGATC 1 cut(s) 483
Ama87I CYCGRG 1 cut(s) 614
AoxI GGCC 2 cut(s) 61, 728
ApeKI GCWGC 2 cut(s) 658, 692
ApoI RAATTY 1 cut(s) 34
AsiGI ACCGGT 1 cut(s) 169
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 165
AvaI CYCGRG 1 cut(s) 614
AvaII GGWCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 721
BauI CACGAG 1 cut(s) 363
BbsI GAAGAC 2 cut(s) 827, 936
Bbv12I GWGCWC 1 cut(s) 721
BbvCI CCTCAGC 1 cut(s) 648
BbvI GCAGC 2 cut(s) 670, 679
BccI CCATC 3 cut(s) 179, 188, 374
BceAI ACGGC 2 cut(s) 76, 500
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 5 cut(s) 36, 354, 402, 750, 792
BfaI CTAG 2 cut(s) 584, 956
BfuAI ACCTGC 1 cut(s) 828
BisI GCNGC 3 cut(s) 434, 659, 693
BlpI GCTNAGC 1 cut(s) 920
BlsI GCNGC 3 cut(s) 435, 660, 694
Bme1390I CCNGG 1 cut(s) 165
Bme18I GGWCC 1 cut(s) 167
BmeT110I CYCGRG 1 cut(s) 614
BmgBI CACGTC 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 2 cut(s) 130, 430
BpiI GAAGAC 2 cut(s) 827, 936
Bpu10I CCTNAGC 1 cut(s) 648
Bpu1102I GCTNAGC 1 cut(s) 920
BpuEI CTTGAG 2 cut(s) 446, 705
BpuMI CCSGG 1 cut(s) 165
Bsa29I ATCGAT 1 cut(s) 450
BsaBI GATNNNNATC 1 cut(s) 417
BsaWI WCCGGW 2 cut(s) 169, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse118I RCCGGY 1 cut(s) 169
Bse8I GATNNNNATC 1 cut(s) 417
BseCI ATCGAT 1 cut(s) 450
BseGI GGATG 3 cut(s) 10, 121, 755
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMII CTCAG 4 cut(s) 108, 131, 305, 639
BseRI GAGGAG 1 cut(s) 593
BseXI GCAGC 2 cut(s) 670, 679
BsgI GTGCAG 2 cut(s) 678, 856
Bsh1236I CGCG 1 cut(s) 423
BshFI GGCC 2 cut(s) 63, 730
BshTI ACCGGT 1 cut(s) 169
BshVI ATCGAT 1 cut(s) 450
BsiHKAI GWGCWC 1 cut(s) 721
BsiHKCI CYCGRG 1 cut(s) 614
BsiSI CCGG 4 cut(s) 74, 164, 170, 336
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 1 cut(s) 80
BsmAI GTCTC 5 cut(s) 36, 354, 402, 750, 792
BsmBI CGTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 2 cut(s) 63, 730
BsoBI CYCGRG 1 cut(s) 614
Bsp1286I GDGCHC 1 cut(s) 721
Bsp143I GATC 4 cut(s) 109, 418, 475, 504
Bsp1720I GCTNAGC 1 cut(s) 920
Bsp68I TCGCGA 1 cut(s) 423
BspACI CCGC 3 cut(s) 51, 91, 433
BspANI GGCC 2 cut(s) 63, 730
BspCNI CTCAG 4 cut(s) 107, 130, 304, 640
BspDI ATCGAT 1 cut(s) 450
BspFNI CGCG 1 cut(s) 423
BspLI GGNNCC 1 cut(s) 168
BspMI ACCTGC 1 cut(s) 828
BspPI GGATC 1 cut(s) 483
BspQI GCTCTTC 1 cut(s) 12
BsrBI CCGCTC 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 169
BssAI RCCGGY 1 cut(s) 169
BssMI GATC 4 cut(s) 109, 418, 475, 504
BssNAI GTATAC 1 cut(s) 970
BssSI CACGAG 1 cut(s) 363
Bst1107I GTATAC 1 cut(s) 970
Bst2BI CACGAG 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 529, 761
Bst6I CTCTTC 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 318, 489
BstDEI CTNAG 6 cut(s) 94, 117, 291, 648, 920, 948
BstF5I GGATG 3 cut(s) 10, 121, 755
BstFNI CGCG 1 cut(s) 423
BstKTI GATC 4 cut(s) 112, 421, 478, 507
BstMAI GTCTC 5 cut(s) 36, 354, 402, 750, 792
BstMBI GATC 4 cut(s) 109, 418, 475, 504
BstMWI GCNNNNNNNGC 2 cut(s) 158, 368
BstNSI RCATGY 2 cut(s) 491, 976
BstSCI CCNGG 1 cut(s) 163
BstUI CGCG 1 cut(s) 423
BstV1I GCAGC 2 cut(s) 670, 679
BstV2I GAAGAC 2 cut(s) 827, 936
BstZ17I GTATAC 1 cut(s) 970
Bsu15I ATCGAT 1 cut(s) 450
BsuRI GGCC 2 cut(s) 63, 730
BsuTUI ATCGAT 1 cut(s) 450
BtrI CACGTC 1 cut(s) 251
BtsCI GGATG 3 cut(s) 10, 121, 755
BtsIMutI CAGTG 1 cut(s) 766
BtuMI TCGCGA 1 cut(s) 423
BveI ACCTGC 1 cut(s) 828
Cac8I GCNNGC 2 cut(s) 318, 489
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 167
ClaI ATCGAT 1 cut(s) 450
Csp6I GTAC 1 cut(s) 673
CspAI ACCGGT 1 cut(s) 169
CviAII CATG 6 cut(s) 107, 146, 488, 654, 879, 973
CviQI GTAC 1 cut(s) 673
DdeI CTNAG 6 cut(s) 94, 117, 291, 648, 920, 948
DpnI GATC 4 cut(s) 111, 420, 477, 506
DpnII GATC 4 cut(s) 109, 418, 475, 504
EaeI YGGCCR 2 cut(s) 61, 728
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 719
Eco24I GRGCYC 1 cut(s) 721
Eco32I GATATC 2 cut(s) 297, 448
Eco47I GGWCC 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 719
Eco88I CYCGRG 1 cut(s) 614
EcoICRI GAGCTC 1 cut(s) 719
EcoRV GATATC 2 cut(s) 297, 448
EcoT38I GRGCYC 1 cut(s) 721
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 6 cut(s) 110, 149, 491, 657, 882, 976
FaqI GGGAC 1 cut(s) 180
FatI CATG 6 cut(s) 106, 145, 487, 653, 878, 972
FauNDI CATATG 1 cut(s) 888
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 1 cut(s) 969
Fnu4HI GCNGC 3 cut(s) 434, 659, 693
FokI GGATG 2 cut(s) 108, 762
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 3 cut(s) 434, 659, 693
FspBI CTAG 2 cut(s) 584, 956
GluI GCNGC 3 cut(s) 434, 659, 693
HaeIII GGCC 2 cut(s) 63, 730
HapII CCGG 4 cut(s) 74, 164, 170, 336
Hin1II CATG 6 cut(s) 110, 149, 491, 657, 882, 976
HincII GTYRAC 1 cut(s) 518
HindII GTYRAC 1 cut(s) 518
HindIII AAGCTT 1 cut(s) 917
HinfI GANTC 4 cut(s) 26, 54, 100, 466
HpaII CCGG 4 cut(s) 74, 164, 170, 336
Hpy166II GTNNAC 2 cut(s) 518, 970
Hpy188I TCNGA 6 cut(s) 41, 97, 193, 294, 379, 558
Hpy188III TCNNGA 7 cut(s) 23, 239, 353, 422, 463, 508, 710
Hpy8I GTNNAC 2 cut(s) 518, 970
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 2 cut(s) 29, 740
HpyCH4III ACNGT 2 cut(s) 529, 761
HpyCH4IV ACGT 2 cut(s) 250, 664
HpyCH4V TGCA 6 cut(s) 161, 316, 443, 668, 695, 837
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 368
HpyF3I CTNAG 6 cut(s) 94, 117, 291, 648, 920, 948
HpySE526I ACGT 2 cut(s) 250, 664
Hsp92II CATG 6 cut(s) 110, 149, 491, 657, 882, 976
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 4 cut(s) 109, 418, 475, 504
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 206, 580
LpnPI CCDG 8 cut(s) 77, 87, 138, 177, 183, 349, 455, 823
Lsp1109I GCAGC 2 cut(s) 670, 679
LweI GCATC 2 cut(s) 130, 430
MaeI CTAG 2 cut(s) 584, 956
MaeII ACGT 2 cut(s) 250, 664
MaeIII GTNAC 3 cut(s) 426, 480, 531
MalI GATC 4 cut(s) 111, 420, 477, 506
MbiI CCGCTC 1 cut(s) 93
MboI GATC 4 cut(s) 109, 418, 475, 504
MboII GAAGA 4 cut(s) 291, 366, 832, 936
MhlI GDGCHC 1 cut(s) 721
MluCI AATT 4 cut(s) 34, 77, 180, 286
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 385
MseI TTAA 1 cut(s) 87
MspA1I CMGCKG 2 cut(s) 433, 634
MspI CCGG 4 cut(s) 74, 164, 170, 336
MspR9I CCNGG 1 cut(s) 165
MvnI CGCG 1 cut(s) 423
MwoI GCNNNNNNNGC 2 cut(s) 158, 368
NciI CCSGG 1 cut(s) 165
NdeI CATATG 1 cut(s) 888
NdeII GATC 4 cut(s) 109, 418, 475, 504
NlaIII CATG 6 cut(s) 110, 149, 491, 657, 882, 976
NlaIV GGNNCC 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 531
NruI TCGCGA 1 cut(s) 423
NspI RCATGY 2 cut(s) 491, 976
PaeI GCATGC 1 cut(s) 491
PaeR7I CTCGAG 1 cut(s) 614
PciI ACATGT 1 cut(s) 972
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 3 cut(s) 54, 100, 466
PinAI ACCGGT 1 cut(s) 169
PkrI GCNGC 3 cut(s) 435, 660, 694
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
PscI ACATGT 1 cut(s) 972
Psp124BI GAGCTC 1 cut(s) 721
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 1 cut(s) 167
PspXI VCTCGAGB 1 cut(s) 614
PvuII CAGCTG 1 cut(s) 634
RruI TCGCGA 1 cut(s) 423
RsaI GTAC 1 cut(s) 674
RsaNI GTAC 1 cut(s) 673
SacI GAGCTC 1 cut(s) 721
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 3 cut(s) 434, 659, 693
Sau3AI GATC 4 cut(s) 109, 418, 475, 504
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 1 cut(s) 721
SfaNI GCATC 2 cut(s) 130, 430
Sfr274I CTCGAG 1 cut(s) 614
SinI GGWCC 1 cut(s) 167
SlaI CTCGAG 1 cut(s) 614
SmlI CTYRAG 3 cut(s) 461, 614, 720
SmoI CTYRAG 3 cut(s) 461, 614, 720
SphI GCATGC 1 cut(s) 491
Sse9I AATT 4 cut(s) 34, 77, 180, 286
SsiI CCGC 3 cut(s) 51, 91, 433
SspMI CTAG 2 cut(s) 584, 956
SstI GAGCTC 1 cut(s) 721
StyD4I CCNGG 1 cut(s) 163
TaaI ACNGT 2 cut(s) 529, 761
TaiI ACGT 2 cut(s) 253, 667
TaqI TCGA 4 cut(s) 57, 450, 498, 615
TaqII GACCGA 2 cut(s) 312, 729
TasI AATT 4 cut(s) 34, 77, 180, 286
TatI WGTACW 1 cut(s) 672
TauI GCSGC 1 cut(s) 436
TfiI GAWTC 3 cut(s) 54, 100, 466
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TscAI CASTG 1 cut(s) 766
TseFI GTSAC 1 cut(s) 531
TseI GCWGC 2 cut(s) 658, 692
Tsp45I GTSAC 1 cut(s) 531
TspDTI ATGAA 1 cut(s) 134
TspGWI ACGGA 1 cut(s) 554
TspRI CASTG 1 cut(s) 766
VpaK11BI GGWCC 1 cut(s) 167
XapI RAATTY 1 cut(s) 34
XceI RCATGY 2 cut(s) 491, 976
XhoI CTCGAG 1 cut(s) 614
XmiI GTMKAC 1 cut(s) 969
XspI CTAG 2 cut(s) 584, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.