Rh6CG489400

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0010150
Physical Location & Seq
Reverse (-)
65712869 .. 65717247
4379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG489400.1

Sequence Viewer

Length: 1032 bp
ATGGCTCTTCCCCAACACCACTTTCAACAACACTATCCGCCCCAACAACAACAACAACAACAACAACAGCAGCAATCCAAGTCATTCAGAAATTTATACACAATCGACGGTCAGATGTCGCCGGCGGTGGCCTATTTCGACTCCAACGCCTTACAAGATCACTCCCAGCATCCGCCTTATGTTCCACCCTTTCATGTAGTAGGGTTTGCGCCTGGTCCCGTGGCTGCAACAGACGGCAGCGACAATGGTGCTGATTTTCAATGGAACTATGGCTTAGAATCCAAGAGGAAAAGGCTCAAGGAGCAGGATTTTTTGGAGAACAACTCGCAAATGTCTTCGGTTCTGCAACAGCAGCAATCAGTCTCAACGGGCTTGGATCTGTCCCTTGACAATAATCGCATGGCGTCTACAGGGGACTCGGCTTTGCTGTCTCTCATTGGCGAGGATGTTGATCTCGAGATACAGCGGCAAGATGTTGAGATTGATAGGTTCCTCAAAGCCCAGAGTGATCGATTGCGACAAAATATCCTGGACAAGGTCCAAGCTGCCCAACTTCAAACTCTCTCAGTTGTAGAAGAAAAAGTACTCCAGAAGCTCCGGGAAAAAGAAGCAGAAGTCGAGAGCATCAACAAGAAGAATATGGAGCTTGAAGAAAGAATGGATCAGTTGAGTCTTGAAGCAAGGATTTGGCAACATCAAGCCAGGAACAATGAACACATGATCACAGCTCTCAAGGTTAATCTTGATCAAGTAATTGCTCAAAGTAGAGACAGCAAGGAAGGATGTGGTGACAGTGAAATAGATGATACAGCTTCCTGCTGCAATGGCCATTCCATCAATTTTAATATGTTTGGCAAGGAGAGCAATGATGTGAAAGAGGTAATGACGTGCAAGGCCTGCAGAGTGAATGAAGTTTGCATGCTTCTATTACCCTGTAAGCATCTTTGCCTGTGTAAAGATTGCGAATGTAAGCTTAGCATTTGTCCTGTGTGTCAGTCCTCTAAGTTTATCGGCATGGAAGTATACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

343

Amino Acids

38.95

Weight (kDa)

5.18

Isoelectric Point (pI)

60.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 295 - 333 8.7e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 407, 1023
AciI CCGC 4 cut(s) 38, 125, 173, 466
AclWI GGATC 2 cut(s) 384, 669
AcoI YGGCCR 1 cut(s) 826
AcsI RAATTY 1 cut(s) 91
AcyI GRCGYC 1 cut(s) 404
AfaI GTAC 1 cut(s) 585
AfiI CCNNNNNNNGG 1 cut(s) 535
AgsI TTSAA 5 cut(s) 26, 260, 557, 650, 677
AjiI CACGTC 1 cut(s) 888
AjnI CCWGG 3 cut(s) 211, 528, 701
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AluBI AGCT 6 cut(s) 545, 595, 646, 728, 812, 973
AluI AGCT 6 cut(s) 545, 595, 646, 728, 812, 973
Alw26I GTCTC 3 cut(s) 367, 435, 762
AlwI GGATC 2 cut(s) 384, 669
Ama87I CYCGRG 1 cut(s) 455
AoxI GGCC 3 cut(s) 129, 826, 894
ApeKI GCWGC 6 cut(s) 70, 224, 237, 352, 545, 819
ApoI RAATTY 1 cut(s) 91
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 2 cut(s) 215, 538
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 1 cut(s) 800
AvaI CYCGRG 1 cut(s) 455
AvaII GGWCC 2 cut(s) 215, 538
BalI TGGCCA 1 cut(s) 828
BarI GAAGNNNNNNTAC 2 cut(s) 567, 599
BbsI GAAGAC 1 cut(s) 327
BbvI GCAGC 6 cut(s) 82, 211, 249, 364, 532, 806
BccI CCATC 1 cut(s) 842
BceAI ACGGC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 3 cut(s) 213, 530, 703
BclI TGATCA 2 cut(s) 720, 745
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 3 cut(s) 367, 435, 762
BfmI CTRYAG 2 cut(s) 408, 898
BisI GCNGC 7 cut(s) 71, 225, 238, 353, 467, 546, 820
BlpI GCTNAGC 1 cut(s) 974
BlsI GCNGC 7 cut(s) 72, 226, 239, 354, 468, 547, 821
BmcAI AGTACT 1 cut(s) 585
Bme1390I CCNGG 4 cut(s) 213, 530, 599, 703
Bme18I GGWCC 2 cut(s) 215, 538
BmeT110I CYCGRG 1 cut(s) 455
BmgBI CACGTC 1 cut(s) 888
BmgT120I GGNCC 2 cut(s) 215, 538
BmiI GGNNCC 2 cut(s) 217, 491
BmrFI CCNGG 4 cut(s) 213, 530, 599, 703
BmsI GCATC 3 cut(s) 178, 633, 949
BpiI GAAGAC 1 cut(s) 327
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 1 cut(s) 572
Bpu1102I GCTNAGC 1 cut(s) 974
BpuEI CTTGAG 2 cut(s) 281, 716
BpuMI CCSGG 1 cut(s) 599
Bsa29I ATCGAT 1 cut(s) 511
BsaBI GATNNNNATC 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 404
BsaJI CCNNGG 1 cut(s) 219
Bsc4I CCNNNNNNNGG 1 cut(s) 535
Bse118I RCCGGY 1 cut(s) 121
Bse3DI GCAATG 2 cut(s) 829, 871
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 3 cut(s) 213, 530, 703
BseCI ATCGAT 1 cut(s) 511
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 3 cut(s) 169, 451, 788
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMI GCAATG 2 cut(s) 829, 871
BseMII CTCAG 1 cut(s) 579
BseXI GCAGC 6 cut(s) 82, 211, 249, 364, 532, 806
BseYI CCCAGC 1 cut(s) 165
BshFI GGCC 3 cut(s) 131, 828, 896
BshVI ATCGAT 1 cut(s) 511
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 2 cut(s) 122, 598
BslFI GGGAC 3 cut(s) 201, 367, 428
BslI CCNNNNNNNGG 1 cut(s) 535
BsmAI GTCTC 3 cut(s) 367, 435, 762
BsmFI GGGAC 3 cut(s) 201, 367, 428
BsnI GGCC 3 cut(s) 131, 828, 896
BsoBI CYCGRG 1 cut(s) 455
Bsp143I GATC 7 cut(s) 157, 376, 451, 508, 661, 720, 745
Bsp1720I GCTNAGC 1 cut(s) 974
BspACI CCGC 4 cut(s) 38, 125, 173, 466
BspANI GGCC 3 cut(s) 131, 828, 896
BspCNI CTCAG 1 cut(s) 578
BspDI ATCGAT 1 cut(s) 511
BspLI GGNNCC 2 cut(s) 217, 491
BspMAI CTGCAG 1 cut(s) 902
BspPI GGATC 2 cut(s) 384, 669
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 829, 871
BsrFI RCCGGY 1 cut(s) 121
BssAI RCCGGY 1 cut(s) 121
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 7 cut(s) 157, 376, 451, 508, 661, 720, 745
BssNAI GTATAC 1 cut(s) 1024
BssNI GRCGYC 1 cut(s) 404
Bst1107I GTATAC 1 cut(s) 1024
Bst2UI CCWGG 3 cut(s) 213, 530, 703
Bst4CI ACNGT 2 cut(s) 110, 794
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 404
BstAPI GCANNNNNTGC 1 cut(s) 897
BstC8I GCNNGC 3 cut(s) 123, 898, 920
BstDEI CTNAG 4 cut(s) 274, 565, 974, 1002
BstDSI CCRYGG 1 cut(s) 219
BstENI CCTNNNNNAGG 1 cut(s) 533
BstF5I GGATG 3 cut(s) 169, 451, 788
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 7 cut(s) 160, 379, 454, 511, 664, 723, 748
BstMAI GTCTC 3 cut(s) 367, 435, 762
BstMBI GATC 7 cut(s) 157, 376, 451, 508, 661, 720, 745
BstMWI GCNNNNNNNGC 5 cut(s) 301, 352, 825, 861, 897
BstNI CCWGG 3 cut(s) 213, 530, 703
BstNSI RCATGY 1 cut(s) 922
BstSCI CCNGG 4 cut(s) 211, 528, 597, 701
BstSFI CTRYAG 2 cut(s) 408, 898
BstV1I GCAGC 6 cut(s) 82, 211, 249, 364, 532, 806
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BstZ17I GTATAC 1 cut(s) 1024
Bsu15I ATCGAT 1 cut(s) 511
BsuRI GGCC 3 cut(s) 131, 828, 896
BsuTUI ATCGAT 1 cut(s) 511
BtgI CCRYGG 1 cut(s) 219
BtrI CACGTC 1 cut(s) 888
BtsCI GGATG 3 cut(s) 169, 451, 788
BtsIMutI CAGTG 1 cut(s) 799
Cac8I GCNNGC 3 cut(s) 123, 898, 920
CfoI GCGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 121
Cfr13I GGNCC 2 cut(s) 215, 538
ClaI ATCGAT 1 cut(s) 511
CseI GACGC 1 cut(s) 393
Csp6I GTAC 1 cut(s) 584
CviAII CATG 5 cut(s) 194, 400, 718, 919, 1015
CviQI GTAC 1 cut(s) 584
DdeI CTNAG 4 cut(s) 274, 565, 974, 1002
DpnI GATC 7 cut(s) 159, 378, 453, 510, 663, 722, 747
DpnII GATC 7 cut(s) 157, 376, 451, 508, 661, 720, 745
EaeI YGGCCR 1 cut(s) 826
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 2 cut(s) 27, 162
Eco147I AGGCCT 1 cut(s) 896
Eco47I GGWCC 2 cut(s) 215, 538
Eco88I CYCGRG 1 cut(s) 455
EcoNI CCTNNNNNAGG 1 cut(s) 533
EcoRII CCWGG 3 cut(s) 211, 528, 701
FaeI CATG 5 cut(s) 197, 403, 721, 922, 1018
FaqI GGGAC 3 cut(s) 201, 367, 428
FatI CATG 5 cut(s) 193, 399, 717, 918, 1014
FbaI TGATCA 2 cut(s) 720, 745
FblI GTMKAC 2 cut(s) 407, 1023
Fnu4HI GCNGC 7 cut(s) 71, 225, 238, 353, 467, 546, 820
FokI GGATG 3 cut(s) 156, 458, 795
Fsp4HI GCNGC 7 cut(s) 71, 225, 238, 353, 467, 546, 820
GlaI GCGC 1 cut(s) 210
GluI GCNGC 7 cut(s) 71, 225, 238, 353, 467, 546, 820
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 3 cut(s) 131, 828, 896
HapII CCGG 2 cut(s) 122, 598
HgaI GACGC 1 cut(s) 393
HhaI GCGC 1 cut(s) 211
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 5 cut(s) 197, 403, 721, 922, 1018
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HindIII AAGCTT 1 cut(s) 971
HinfI GANTC 4 cut(s) 140, 278, 416, 670
HpaII CCGG 2 cut(s) 122, 598
HphI GGTGA 1 cut(s) 800
Hpy166II GTNNAC 2 cut(s) 408, 1024
Hpy188I TCNGA 2 cut(s) 89, 114
Hpy188III TCNNGA 6 cut(s) 455, 457, 589, 619, 674, 743
Hpy8I GTNNAC 2 cut(s) 408, 1024
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 773
HpyCH4III ACNGT 2 cut(s) 110, 794
HpyCH4IV ACGT 1 cut(s) 887
HpyCH4V TGCA 6 cut(s) 227, 346, 822, 891, 900, 918
HpyF10VI GCNNNNNNNGC 5 cut(s) 301, 352, 825, 861, 897
HpyF3I CTNAG 4 cut(s) 274, 565, 974, 1002
HpySE526I ACGT 1 cut(s) 887
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 5 cut(s) 197, 403, 721, 922, 1018
HspAI GCGC 1 cut(s) 209
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Ksp22I TGATCA 2 cut(s) 720, 745
Kzo9I GATC 7 cut(s) 157, 376, 451, 508, 661, 720, 745
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 301, 600, 643
Lsp1109I GCAGC 6 cut(s) 82, 211, 249, 364, 532, 806
LweI GCATC 3 cut(s) 178, 633, 949
MaeII ACGT 1 cut(s) 887
MaeIII GTNAC 1 cut(s) 788
MalI GATC 7 cut(s) 159, 378, 453, 510, 663, 722, 747
MboI GATC 7 cut(s) 157, 376, 451, 508, 661, 720, 745
MboII GAAGA 4 cut(s) 327, 587, 646, 662
MflI RGATCY 1 cut(s) 376
MlsI TGGCCA 1 cut(s) 828
MluCI AATT 3 cut(s) 91, 753, 838
MluNI TGGCCA 1 cut(s) 828
MlyI GAGTC 3 cut(s) 134, 410, 679
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 5 cut(s) 279, 436, 503, 871, 1009
Mox20I TGGCCA 1 cut(s) 828
MreI CGCCGGCG 1 cut(s) 121
MroNI GCCGGC 1 cut(s) 121
MscI TGGCCA 1 cut(s) 828
MseI TTAA 3 cut(s) 738, 843, 1030
Msp20I TGGCCA 1 cut(s) 828
MspA1I CMGCKG 1 cut(s) 466
MspI CCGG 2 cut(s) 122, 598
MspR9I CCNGG 4 cut(s) 213, 530, 599, 703
MvaI CCWGG 3 cut(s) 213, 530, 703
MwoI GCNNNNNNNGC 5 cut(s) 301, 352, 825, 861, 897
NaeI GCCGGC 1 cut(s) 123
NciI CCSGG 1 cut(s) 599
NdeII GATC 7 cut(s) 157, 376, 451, 508, 661, 720, 745
NgoMIV GCCGGC 1 cut(s) 121
NlaIII CATG 5 cut(s) 197, 403, 721, 922, 1018
NlaIV GGNNCC 2 cut(s) 217, 491
NmeAIII GCCGAG 1 cut(s) 398
NmuCI GTSAC 1 cut(s) 788
NspI RCATGY 1 cut(s) 922
PaeI GCATGC 1 cut(s) 922
PaeR7I CTCGAG 1 cut(s) 455
PceI AGGCCT 1 cut(s) 896
PciSI GCTCTTC 1 cut(s) 12
PdiI GCCGGC 1 cut(s) 123
PfeI GAWTC 1 cut(s) 278
PflFI GACNNNGTC 1 cut(s) 536
PfoI TCCNGGA 2 cut(s) 528, 597
PkrI GCNGC 7 cut(s) 72, 226, 239, 354, 468, 547, 821
PleI GAGTC 3 cut(s) 134, 410, 678
PpsI GAGTC 3 cut(s) 134, 410, 678
Psp6I CCWGG 3 cut(s) 211, 528, 701
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 3 cut(s) 211, 528, 701
PspN4I GGNNCC 2 cut(s) 217, 491
PspPI GGNCC 2 cut(s) 215, 538
PstI CTGCAG 1 cut(s) 902
PsuI RGATCY 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 536
RsaI GTAC 1 cut(s) 585
RsaNI GTAC 1 cut(s) 584
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 738, 843, 1030
SatI GCNGC 7 cut(s) 71, 225, 238, 353, 467, 546, 820
Sau3AI GATC 7 cut(s) 157, 376, 451, 508, 661, 720, 745
Sau96I GGNCC 2 cut(s) 215, 538
ScaI AGTACT 1 cut(s) 585
SchI GAGTC 3 cut(s) 134, 410, 679
ScrFI CCNGG 4 cut(s) 213, 530, 599, 703
SfaNI GCATC 3 cut(s) 178, 633, 949
SfcI CTRYAG 2 cut(s) 408, 898
Sfr274I CTCGAG 1 cut(s) 455
SgrAI CRCCGGYG 1 cut(s) 121
SinI GGWCC 2 cut(s) 215, 538
SlaI CTCGAG 1 cut(s) 455
SmlI CTYRAG 3 cut(s) 296, 455, 731
SmoI CTYRAG 3 cut(s) 296, 455, 731
SphI GCATGC 1 cut(s) 922
Sse9I AATT 3 cut(s) 91, 753, 838
SseBI AGGCCT 1 cut(s) 896
SsiI CCGC 4 cut(s) 38, 125, 173, 466
StuI AGGCCT 1 cut(s) 896
StyD4I CCNGG 4 cut(s) 211, 528, 597, 701
TaaI ACNGT 2 cut(s) 110, 794
TaiI ACGT 1 cut(s) 890
TaqI TCGA 5 cut(s) 105, 138, 456, 511, 618
TasI AATT 3 cut(s) 91, 753, 838
TatI WGTACW 1 cut(s) 583
TauI GCSGC 1 cut(s) 469
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 3 cut(s) 738, 843, 1030
Tru9I TTAA 3 cut(s) 738, 843, 1030
TscAI CASTG 1 cut(s) 799
TseFI GTSAC 1 cut(s) 788
TseI GCWGC 6 cut(s) 70, 224, 237, 352, 545, 819
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 3 cut(s) 182, 726, 924
TspRI CASTG 1 cut(s) 799
Tth111I GACNNNGTC 1 cut(s) 536
VpaK11BI GGWCC 2 cut(s) 215, 538
XagI CCTNNNNNAGG 1 cut(s) 533
XapI RAATTY 1 cut(s) 91
XceI RCATGY 1 cut(s) 922
XhoI CTCGAG 1 cut(s) 455
XmiI GTMKAC 2 cut(s) 407, 1023
ZrmI AGTACT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.