FvH4_2g34250

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010150
Physical Location & Seq
Forward (+)
25482610 .. 25486470
3861 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34250.t1

Sequence Viewer

Length: 1044 bp
ATGGCTCTTCCCCAACACCACTTTCAACAACACTACCAGCCCCAACAACAGCAACAGCAACAGCAACAGCAACAGCAAACGCAATCCAAGTCATTCAGAAATTTGTACGCGATCGACGGTCAGATGTCGCCGGCGGTGGCCTACTTCGACCCCAACGCCTTGCAAGATCACTCCCAGCACCCGCCTTCGCCTTATGTTCCCCCCTTTCATGTAGTAGGGTTTGCACCGGGTCCGGTGGCTGCAACAGACGGCAGTGACAATGGTGCTGATTTTCAGTGGAACTATGGCTTAGAATCCAAGAGGAAAAAGCTCAAGGAGCAGGATTTCTTGGAGAACAACTCGCAGATGTCGTCGGTTTTGCAGCAGCAATCAGTCTCGACAGGCTTGGGTCTGTCCCTTGACAATAATCGCATGGCGTCTACAGGGGATTCAGCTTTGCTGTCTCTCATTGGCGAGGATATTGATCTTGAGATACAGCGGCAGGATTCTGAAATTGATAGGTTCCTCAAAGACCAGAGTGATCGTTTGCGACAAAATATCCTGGACAAGGTCCAAGCTGCCCAACTTCAAACTCTCTCAGTTGTAGAAGAAAAGGTACTCCAGAAACTCCGGGAAAAAGAAGCAGAAGTGGAAAGCATCAACAAGAAGAATATGGAGCTTGAAAAACGAATGAATCAGTTGGATCTTGAAGCCAGGATTTGGCAACATCAAGCCAGCAACAATGAACACATGATCGCAGCTCTCAAGGATAATCTTGATCAAGTAATTGCTCAAAGTAGAGACAGCAAAGAAGGATGTGGTGACAGTGAAATAGATGATACAGCTTCCTGCTGCAATGGCCGTTCCATCAATTTCAATATGTTCGGCAAGCAGAGCAATGATATGAAAGAGGTGATGACGTGCAAGGCCTGCAGAATGAATGAAGTGTGCATGCTTCTATTACCTTGTAAGCATCTTTGCCTGTGTAAAGACTGCGAAAGTAAGCTCAGCGTTTGTCCTCTGTGTCAGTCCTCTAAGTTTATCGGCATGGAAGTATTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

348

Amino Acids

39.32

Weight (kDa)

5.26

Isoelectric Point (pI)

63.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 299 - 337 1.6e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 699
AccI GTMKAC 1 cut(s) 419
AccII CGCG 1 cut(s) 110
AciI CCGC 3 cut(s) 134, 182, 478
AclWI GGATC 1 cut(s) 690
AcoI YGGCCR 1 cut(s) 838
AcsI RAATTY 1 cut(s) 100
AcyI GRCGYC 1 cut(s) 416
AfaI GTAC 2 cut(s) 107, 597
AfiI CCNNNNNNNGG 2 cut(s) 547, 699
AgsI TTSAA 5 cut(s) 26, 569, 662, 689, 856
AjiI CACGTC 1 cut(s) 900
AjnI CCWGG 2 cut(s) 540, 692
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AluBI AGCT 7 cut(s) 310, 434, 557, 658, 740, 824, 985
AluI AGCT 7 cut(s) 310, 434, 557, 658, 740, 824, 985
Alw26I GTCTC 3 cut(s) 379, 447, 774
AlwI GGATC 1 cut(s) 690
AoxI GGCC 3 cut(s) 138, 838, 906
ApeKI GCWGC 6 cut(s) 239, 361, 364, 557, 737, 831
ApoI RAATTY 1 cut(s) 100
AspS9I GGNCC 2 cut(s) 230, 550
AsuC2I CCSGG 2 cut(s) 228, 611
AsuHPI GGTGA 2 cut(s) 812, 904
AvaII GGWCC 2 cut(s) 230, 550
BarI GAAGNNNNNNTAC 2 cut(s) 579, 611
BbvI GCAGC 6 cut(s) 226, 373, 376, 544, 749, 818
BccI CCATC 1 cut(s) 854
BceAI ACGGC 2 cut(s) 265, 825
BciT130I CCWGG 2 cut(s) 542, 694
BclI TGATCA 1 cut(s) 757
BcnI CCSGG 2 cut(s) 228, 611
BcoDI GTCTC 3 cut(s) 379, 447, 774
BfmI CTRYAG 2 cut(s) 420, 910
BisI GCNGC 7 cut(s) 240, 362, 365, 479, 558, 738, 832
BlpI GCTNAGC 1 cut(s) 986
BlsI GCNGC 7 cut(s) 241, 363, 366, 480, 559, 739, 833
Bme1390I CCNGG 4 cut(s) 228, 542, 611, 694
Bme18I GGWCC 2 cut(s) 230, 550
BmgBI CACGTC 1 cut(s) 900
BmgT120I GGNCC 2 cut(s) 230, 550
BmiI GGNNCC 2 cut(s) 231, 503
BmrFI CCNGG 4 cut(s) 228, 542, 611, 694
BmsI GCATC 2 cut(s) 645, 961
BplI GAGNNNNNCTC 2 cut(s) 323, 355
BpmI CTGGAG 1 cut(s) 584
Bpu1102I GCTNAGC 1 cut(s) 986
BpuEI CTTGAG 3 cut(s) 296, 488, 728
BpuMI CCSGG 2 cut(s) 228, 611
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 416
BsaWI WCCGGW 1 cut(s) 232
Bsc4I CCNNNNNNNGG 2 cut(s) 547, 699
Bse118I RCCGGY 1 cut(s) 130
Bse3DI GCAATG 2 cut(s) 841, 883
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 2 cut(s) 542, 694
BseGI GGATG 1 cut(s) 800
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 2 cut(s) 547, 699
BseMI GCAATG 2 cut(s) 841, 883
BseMII CTCAG 2 cut(s) 591, 1000
BseXI GCAGC 6 cut(s) 226, 373, 376, 544, 749, 818
BseYI CCCAGC 1 cut(s) 174
Bsh1236I CGCG 1 cut(s) 110
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 3 cut(s) 140, 840, 908
BsiEI CGRYCG 1 cut(s) 114
BsiSI CCGG 4 cut(s) 131, 227, 233, 610
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 547, 699
BsmAI GTCTC 3 cut(s) 379, 447, 774
BsmFI GGGAC 1 cut(s) 379
BsnI GGCC 3 cut(s) 140, 840, 908
Bsp143I GATC 7 cut(s) 111, 166, 463, 520, 682, 732, 757
Bsp1720I GCTNAGC 1 cut(s) 986
BspACI CCGC 3 cut(s) 134, 182, 478
BspANI GGCC 3 cut(s) 140, 840, 908
BspCNI CTCAG 2 cut(s) 590, 999
BspFNI CGCG 1 cut(s) 110
BspLI GGNNCC 2 cut(s) 231, 503
BspMAI CTGCAG 1 cut(s) 914
BspPI GGATC 1 cut(s) 690
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 841, 883
BsrFI RCCGGY 1 cut(s) 130
BssAI RCCGGY 1 cut(s) 130
BssMI GATC 7 cut(s) 111, 166, 463, 520, 682, 732, 757
BssNI GRCGYC 1 cut(s) 416
Bst2UI CCWGG 2 cut(s) 542, 694
Bst4CI ACNGT 2 cut(s) 119, 806
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 909
BstC8I GCNNGC 5 cut(s) 132, 715, 869, 910, 932
BstDEI CTNAG 4 cut(s) 289, 577, 986, 1014
BstENI CCTNNNNNAGG 1 cut(s) 545
BstF5I GGATG 1 cut(s) 800
BstFNI CGCG 1 cut(s) 110
BstKTI GATC 7 cut(s) 114, 169, 466, 523, 685, 735, 760
BstMAI GTCTC 3 cut(s) 379, 447, 774
BstMBI GATC 7 cut(s) 111, 166, 463, 520, 682, 732, 757
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 4 cut(s) 316, 837, 873, 909
BstNI CCWGG 2 cut(s) 542, 694
BstNSI RCATGY 1 cut(s) 934
BstSCI CCNGG 4 cut(s) 226, 540, 609, 692
BstSFI CTRYAG 2 cut(s) 420, 910
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 6 cut(s) 226, 373, 376, 544, 749, 818
BstX2I RGATCY 1 cut(s) 682
BstYI RGATCY 1 cut(s) 682
BsuRI GGCC 3 cut(s) 140, 840, 908
BtrI CACGTC 1 cut(s) 900
BtsCI GGATG 1 cut(s) 800
BtsI GCAGTG 1 cut(s) 259
BtsIMutI CAGTG 3 cut(s) 259, 281, 811
Cac8I GCNNGC 5 cut(s) 132, 715, 869, 910, 932
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 2 cut(s) 230, 550
CseI GACGC 1 cut(s) 405
Csp6I GTAC 2 cut(s) 106, 596
CviAII CATG 5 cut(s) 209, 412, 730, 931, 1027
CviQI GTAC 2 cut(s) 106, 596
DdeI CTNAG 4 cut(s) 289, 577, 986, 1014
DpnI GATC 7 cut(s) 113, 168, 465, 522, 684, 734, 759
DpnII GATC 7 cut(s) 111, 166, 463, 520, 682, 732, 757
EaeI YGGCCR 1 cut(s) 838
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco147I AGGCCT 1 cut(s) 908
Eco47I GGWCC 2 cut(s) 230, 550
EcoNI CCTNNNNNAGG 1 cut(s) 545
EcoRII CCWGG 2 cut(s) 540, 692
FaeI CATG 5 cut(s) 212, 415, 733, 934, 1030
FaqI GGGAC 1 cut(s) 379
FatI CATG 5 cut(s) 208, 411, 729, 930, 1026
FauI CCCGC 1 cut(s) 189
FbaI TGATCA 1 cut(s) 757
FblI GTMKAC 1 cut(s) 419
Fnu4HI GCNGC 7 cut(s) 240, 362, 365, 479, 558, 738, 832
FokI GGATG 1 cut(s) 807
Fsp4HI GCNGC 7 cut(s) 240, 362, 365, 479, 558, 738, 832
GluI GCNGC 7 cut(s) 240, 362, 365, 479, 558, 738, 832
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 1 cut(s) 584
HaeIII GGCC 3 cut(s) 140, 840, 908
HapII CCGG 4 cut(s) 131, 227, 233, 610
HgaI GACGC 1 cut(s) 405
Hin1I GRCGYC 1 cut(s) 416
Hin1II CATG 5 cut(s) 212, 415, 733, 934, 1030
HinfI GANTC 4 cut(s) 293, 428, 485, 673
HpaII CCGG 4 cut(s) 131, 227, 233, 610
HphI GGTGA 2 cut(s) 812, 904
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 3 cut(s) 98, 123, 490
Hpy188III TCNNGA 5 cut(s) 376, 467, 601, 686, 755
Hpy8I GTNNAC 1 cut(s) 420
Hpy99I CGWCG 2 cut(s) 119, 355
HpyAV CCTTC 2 cut(s) 195, 785
HpyCH4III ACNGT 2 cut(s) 119, 806
HpyCH4IV ACGT 1 cut(s) 899
HpyCH4V TGCA 8 cut(s) 163, 224, 242, 361, 834, 903, 912, 930
HpyF10VI GCNNNNNNNGC 4 cut(s) 316, 837, 873, 909
HpyF3I CTNAG 4 cut(s) 289, 577, 986, 1014
HpySE526I ACGT 1 cut(s) 899
Hsp92I GRCGYC 1 cut(s) 416
Hsp92II CATG 5 cut(s) 212, 415, 733, 934, 1030
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Ksp22I TGATCA 1 cut(s) 757
Kzo9I GATC 7 cut(s) 111, 166, 463, 520, 682, 732, 757
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 316, 655
Lsp1109I GCAGC 6 cut(s) 226, 373, 376, 544, 749, 818
LweI GCATC 2 cut(s) 645, 961
MaeII ACGT 1 cut(s) 899
MaeIII GTNAC 2 cut(s) 254, 800
MalI GATC 7 cut(s) 113, 168, 465, 522, 684, 734, 759
MboI GATC 7 cut(s) 111, 166, 463, 520, 682, 732, 757
MboII GAAGA 2 cut(s) 599, 658
MflI RGATCY 1 cut(s) 682
MluCI AATT 4 cut(s) 100, 492, 765, 850
MmeI TCCRAC 1 cut(s) 660
MnlI CCTC 6 cut(s) 294, 448, 515, 883, 1008, 1021
MreI CGCCGGCG 1 cut(s) 130
MroNI GCCGGC 1 cut(s) 130
MseI TTAA 1 cut(s) 1042
MspA1I CMGCKG 1 cut(s) 478
MspI CCGG 4 cut(s) 131, 227, 233, 610
MspR9I CCNGG 4 cut(s) 228, 542, 611, 694
MvaI CCWGG 2 cut(s) 542, 694
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 4 cut(s) 316, 837, 873, 909
NaeI GCCGGC 1 cut(s) 132
NciI CCSGG 2 cut(s) 228, 611
NdeII GATC 7 cut(s) 111, 166, 463, 520, 682, 732, 757
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 5 cut(s) 212, 415, 733, 934, 1030
NlaIV GGNNCC 2 cut(s) 231, 503
NmuCI GTSAC 2 cut(s) 254, 800
NspI RCATGY 1 cut(s) 934
PaeI GCATGC 1 cut(s) 934
PceI AGGCCT 1 cut(s) 908
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 347
PdiI GCCGGC 1 cut(s) 132
PfeI GAWTC 4 cut(s) 293, 428, 485, 673
PflFI GACNNNGTC 1 cut(s) 548
PflMI CCANNNNNTGG 1 cut(s) 699
PfoI TCCNGGA 2 cut(s) 540, 609
PkrI GCNGC 7 cut(s) 241, 363, 366, 480, 559, 739, 833
Ple19I CGATCG 1 cut(s) 114
Psp6I CCWGG 2 cut(s) 540, 692
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 2 cut(s) 540, 692
PspN4I GGNNCC 2 cut(s) 231, 503
PspPI GGNCC 2 cut(s) 230, 550
PstI CTGCAG 1 cut(s) 914
PsuI RGATCY 1 cut(s) 682
PsyI GACNNNGTC 1 cut(s) 548
PvuI CGATCG 1 cut(s) 114
RsaI GTAC 2 cut(s) 107, 597
RsaNI GTAC 2 cut(s) 106, 596
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 1042
SatI GCNGC 7 cut(s) 240, 362, 365, 479, 558, 738, 832
Sau3AI GATC 7 cut(s) 111, 166, 463, 520, 682, 732, 757
Sau96I GGNCC 2 cut(s) 230, 550
ScrFI CCNGG 4 cut(s) 228, 542, 611, 694
SfaNI GCATC 2 cut(s) 645, 961
SfcI CTRYAG 2 cut(s) 420, 910
SgrAI CRCCGGYG 1 cut(s) 130
SinI GGWCC 2 cut(s) 230, 550
SmlI CTYRAG 3 cut(s) 311, 467, 743
SmoI CTYRAG 3 cut(s) 311, 467, 743
SphI GCATGC 1 cut(s) 934
Sse9I AATT 4 cut(s) 100, 492, 765, 850
SseBI AGGCCT 1 cut(s) 908
SsiI CCGC 3 cut(s) 134, 182, 478
StuI AGGCCT 1 cut(s) 908
StyD4I CCNGG 4 cut(s) 226, 540, 609, 692
TaaI ACNGT 2 cut(s) 119, 806
TaiI ACGT 1 cut(s) 902
TaqI TCGA 3 cut(s) 114, 147, 377
TasI AATT 4 cut(s) 100, 492, 765, 850
TauI GCSGC 1 cut(s) 481
TfiI GAWTC 4 cut(s) 293, 428, 485, 673
Tru1I TTAA 1 cut(s) 1042
Tru9I TTAA 1 cut(s) 1042
TscAI CASTG 3 cut(s) 259, 281, 811
TseFI GTSAC 2 cut(s) 254, 800
TseI GCWGC 6 cut(s) 239, 361, 364, 557, 737, 831
Tsp45I GTSAC 2 cut(s) 254, 800
TspDTI ATGAA 6 cut(s) 197, 686, 738, 899, 932, 936
TspRI CASTG 3 cut(s) 259, 281, 811
Tth111I GACNNNGTC 1 cut(s) 548
Van91I CCANNNNNTGG 1 cut(s) 699
VpaK11BI GGWCC 2 cut(s) 230, 550
XagI CCTNNNNNAGG 1 cut(s) 545
XapI RAATTY 1 cut(s) 100
XceI RCATGY 1 cut(s) 934
XmiI GTMKAC 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.