RchiOBHm_Chr6g0308171

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010150
Physical Location & Seq
Reverse (-)
66270724 .. 66274025
3302 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27707

Sequence Viewer

Length: 1017 bp
ATGGCTCTTCCCCAACACCACTTTCAACAACACTATCCACCCCAACAACAACAACAGAAGCAATCCAAGTCATTCAGAAATTTATACACAATCGACGGTCAGATGTCGCCGGCGGTGGCCTATTTCGACCCCAACGCCTTACAAGATCACTCCCAGCATCCGCCTTATGTTCCACCTTTTCATGTAGTAGGGTTTGCGCCTGGTCCGGTGGCTGCAACAGATGGCAGTGACAATGGTGCTGATTTTCAATGGAACTATGGCTTAGAATCCAAAAAGAAAAGGCTCAAGGAGCAGGATTTTTTGGAGAACAACTCGCAAATGTCTTCGGTTCTGCAACAGCAATCAGTCTCAACGGGCTTGGGTCTGTCCCTTGACAATAATCGCATGGCGTCTACAGGCGACTCGGCTTTGCTGTCTCTCATTGGCGAGGATGTTGATCTCGAGATACAGCGGCAAGATGTTGAGATTGATAGGTTCCTCAAAGCCCAGAGTGATCGATTGCGACAAAATATCCTGGACAAGGTCCAAGCTGCCCAACTTCAAACTCTCTCAGTTGTAGAAGAAAAAGTACTCCAGAAGCTCCGGGAAAAAGAAGCAGAAGTCGAGAGCATCAACAAGAAGAATATGGAGCTTGAAGAAAGAATGGATCAGTTGAGTCTTGAAGCAAGGATTTGGCAACATCAAGCCAGGAACAATGAACACATGATCACAGCTCTCAAGGTTAATCTTGATCAAGTAATTGCTCAAAGTAGAGACAGCAAGGAAGGATGTGGTGACAGTGAAATAGATGATACAGCTTCCTGCTGCAATGGCCATTCCATCAATTTTAATATGTTTGGCAAGGAGAGCAATGATGTGAAAGAGGTAATGACGTGCAAGGCCTGCAGAGTGAATGAAGTTTGCATGCTTCTATTACCCTGTAAGCATCTTTGCCTGTGTAAAGATTGCGAATGTAAGCTTAGCATTTGTCCTCTGTGTCAGTCCTCTAAGTTTATCGGCATGGAAGTATACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

338

Amino Acids

38.24

Weight (kDa)

5.34

Isoelectric Point (pI)

55.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 290 - 328 9.2e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 392, 1008
AciI CCGC 3 cut(s) 113, 161, 451
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 1 cut(s) 79
AcyI GRCGYC 1 cut(s) 389
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 1 cut(s) 520
AgsI TTSAA 5 cut(s) 26, 248, 542, 635, 662
AjiI CACGTC 1 cut(s) 873
AjnI CCWGG 3 cut(s) 199, 513, 686
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AluBI AGCT 6 cut(s) 530, 580, 631, 713, 797, 958
AluI AGCT 6 cut(s) 530, 580, 631, 713, 797, 958
Alw26I GTCTC 3 cut(s) 352, 420, 747
AlwI GGATC 1 cut(s) 654
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 3 cut(s) 117, 811, 879
ApeKI GCWGC 3 cut(s) 212, 530, 804
ApoI RAATTY 1 cut(s) 79
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 2 cut(s) 203, 523
AsuC2I CCSGG 1 cut(s) 584
AsuHPI GGTGA 1 cut(s) 785
AvaI CYCGRG 1 cut(s) 440
AvaII GGWCC 2 cut(s) 203, 523
BalI TGGCCA 1 cut(s) 813
BarI GAAGNNNNNNTAC 2 cut(s) 552, 584
BbsI GAAGAC 1 cut(s) 315
BbvI GCAGC 3 cut(s) 199, 517, 791
BccI CCATC 2 cut(s) 215, 827
BciT130I CCWGG 3 cut(s) 201, 515, 688
BclI TGATCA 2 cut(s) 705, 730
BcnI CCSGG 1 cut(s) 584
BcoDI GTCTC 3 cut(s) 352, 420, 747
BfmI CTRYAG 2 cut(s) 393, 883
BisI GCNGC 4 cut(s) 213, 452, 531, 805
BlpI GCTNAGC 1 cut(s) 959
BlsI GCNGC 4 cut(s) 214, 453, 532, 806
BmcAI AGTACT 1 cut(s) 570
Bme1390I CCNGG 4 cut(s) 201, 515, 584, 688
Bme18I GGWCC 2 cut(s) 203, 523
BmeT110I CYCGRG 1 cut(s) 440
BmgBI CACGTC 1 cut(s) 873
BmgT120I GGNCC 2 cut(s) 203, 523
BmiI GGNNCC 1 cut(s) 476
BmrFI CCNGG 4 cut(s) 201, 515, 584, 688
BmsI GCATC 3 cut(s) 166, 618, 934
BpiI GAAGAC 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpmI CTGGAG 1 cut(s) 557
Bpu1102I GCTNAGC 1 cut(s) 959
BpuEI CTTGAG 2 cut(s) 269, 701
BpuMI CCSGG 1 cut(s) 584
Bsa29I ATCGAT 1 cut(s) 496
BsaBI GATNNNNATC 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 389
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 1 cut(s) 520
Bse118I RCCGGY 1 cut(s) 109
Bse3DI GCAATG 2 cut(s) 814, 856
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 3 cut(s) 201, 515, 688
BseCI ATCGAT 1 cut(s) 496
BseGI GGATG 3 cut(s) 157, 436, 773
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 1 cut(s) 520
BseMI GCAATG 2 cut(s) 814, 856
BseMII CTCAG 1 cut(s) 564
BseXI GCAGC 3 cut(s) 199, 517, 791
BseYI CCCAGC 1 cut(s) 153
BshFI GGCC 3 cut(s) 119, 813, 881
BshVI ATCGAT 1 cut(s) 496
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 3 cut(s) 110, 206, 583
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 1 cut(s) 520
BsmAI GTCTC 3 cut(s) 352, 420, 747
BsmFI GGGAC 1 cut(s) 352
BsnI GGCC 3 cut(s) 119, 813, 881
BsoBI CYCGRG 1 cut(s) 440
Bsp143I GATC 6 cut(s) 145, 436, 493, 646, 705, 730
Bsp1720I GCTNAGC 1 cut(s) 959
BspACI CCGC 3 cut(s) 113, 161, 451
BspANI GGCC 3 cut(s) 119, 813, 881
BspCNI CTCAG 1 cut(s) 563
BspDI ATCGAT 1 cut(s) 496
BspLI GGNNCC 1 cut(s) 476
BspMAI CTGCAG 1 cut(s) 887
BspPI GGATC 1 cut(s) 654
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 814, 856
BsrFI RCCGGY 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 109
BssMI GATC 6 cut(s) 145, 436, 493, 646, 705, 730
BssNAI GTATAC 1 cut(s) 1009
BssNI GRCGYC 1 cut(s) 389
Bst1107I GTATAC 1 cut(s) 1009
Bst2UI CCWGG 3 cut(s) 201, 515, 688
Bst4CI ACNGT 2 cut(s) 98, 779
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 389
BstAPI GCANNNNNTGC 1 cut(s) 882
BstC8I GCNNGC 3 cut(s) 111, 883, 905
BstDEI CTNAG 4 cut(s) 262, 550, 959, 987
BstENI CCTNNNNNAGG 1 cut(s) 518
BstF5I GGATG 3 cut(s) 157, 436, 773
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 6 cut(s) 148, 439, 496, 649, 708, 733
BstMAI GTCTC 3 cut(s) 352, 420, 747
BstMBI GATC 6 cut(s) 145, 436, 493, 646, 705, 730
BstMWI GCNNNNNNNGC 4 cut(s) 289, 810, 846, 882
BstNI CCWGG 3 cut(s) 201, 515, 688
BstNSI RCATGY 1 cut(s) 907
BstSCI CCNGG 4 cut(s) 199, 513, 582, 686
BstSFI CTRYAG 2 cut(s) 393, 883
BstV1I GCAGC 3 cut(s) 199, 517, 791
BstV2I GAAGAC 1 cut(s) 315
BstZ17I GTATAC 1 cut(s) 1009
Bsu15I ATCGAT 1 cut(s) 496
BsuRI GGCC 3 cut(s) 119, 813, 881
BsuTUI ATCGAT 1 cut(s) 496
BtrI CACGTC 1 cut(s) 873
BtsCI GGATG 3 cut(s) 157, 436, 773
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 2 cut(s) 232, 784
Cac8I GCNNGC 3 cut(s) 111, 883, 905
CfoI GCGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 203, 523
ClaI ATCGAT 1 cut(s) 496
CseI GACGC 1 cut(s) 378
Csp6I GTAC 1 cut(s) 569
CviAII CATG 5 cut(s) 182, 385, 703, 904, 1000
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 4 cut(s) 262, 550, 959, 987
DpnI GATC 6 cut(s) 147, 438, 495, 648, 707, 732
DpnII GATC 6 cut(s) 145, 436, 493, 646, 705, 730
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 881
Eco47I GGWCC 2 cut(s) 203, 523
Eco88I CYCGRG 1 cut(s) 440
EcoNI CCTNNNNNAGG 1 cut(s) 518
EcoRII CCWGG 3 cut(s) 199, 513, 686
FaeI CATG 5 cut(s) 185, 388, 706, 907, 1003
FaqI GGGAC 1 cut(s) 352
FatI CATG 5 cut(s) 181, 384, 702, 903, 999
FbaI TGATCA 2 cut(s) 705, 730
FblI GTMKAC 2 cut(s) 392, 1008
Fnu4HI GCNGC 4 cut(s) 213, 452, 531, 805
FokI GGATG 3 cut(s) 144, 443, 780
Fsp4HI GCNGC 4 cut(s) 213, 452, 531, 805
GlaI GCGC 1 cut(s) 198
GluI GCNGC 4 cut(s) 213, 452, 531, 805
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 3 cut(s) 119, 813, 881
HapII CCGG 3 cut(s) 110, 206, 583
HgaI GACGC 1 cut(s) 378
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 1 cut(s) 389
Hin1II CATG 5 cut(s) 185, 388, 706, 907, 1003
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HindIII AAGCTT 1 cut(s) 956
HinfI GANTC 3 cut(s) 266, 401, 655
HpaII CCGG 3 cut(s) 110, 206, 583
HphI GGTGA 1 cut(s) 785
Hpy166II GTNNAC 2 cut(s) 393, 1009
Hpy188I TCNGA 2 cut(s) 77, 102
Hpy188III TCNNGA 6 cut(s) 440, 442, 574, 604, 659, 728
Hpy8I GTNNAC 2 cut(s) 393, 1009
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 1 cut(s) 758
HpyCH4III ACNGT 2 cut(s) 98, 779
HpyCH4IV ACGT 1 cut(s) 872
HpyCH4V TGCA 6 cut(s) 215, 334, 807, 876, 885, 903
HpyF10VI GCNNNNNNNGC 4 cut(s) 289, 810, 846, 882
HpyF3I CTNAG 4 cut(s) 262, 550, 959, 987
HpySE526I ACGT 1 cut(s) 872
Hsp92I GRCGYC 1 cut(s) 389
Hsp92II CATG 5 cut(s) 185, 388, 706, 907, 1003
HspAI GCGC 1 cut(s) 197
KroI GCCGGC 1 cut(s) 109
KroNI GCCGGC 1 cut(s) 111
Ksp22I TGATCA 2 cut(s) 705, 730
Kzo9I GATC 6 cut(s) 145, 436, 493, 646, 705, 730
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 289, 585, 628
Lsp1109I GCAGC 3 cut(s) 199, 517, 791
LweI GCATC 3 cut(s) 166, 618, 934
MaeII ACGT 1 cut(s) 872
MaeIII GTNAC 2 cut(s) 227, 773
MalI GATC 6 cut(s) 147, 438, 495, 648, 707, 732
MboI GATC 6 cut(s) 145, 436, 493, 646, 705, 730
MboII GAAGA 4 cut(s) 315, 572, 631, 647
MlsI TGGCCA 1 cut(s) 813
MluCI AATT 3 cut(s) 79, 738, 823
MluNI TGGCCA 1 cut(s) 813
MlyI GAGTC 2 cut(s) 395, 664
MnlI CCTC 5 cut(s) 421, 488, 856, 981, 994
Mox20I TGGCCA 1 cut(s) 813
MreI CGCCGGCG 1 cut(s) 109
MroNI GCCGGC 1 cut(s) 109
MscI TGGCCA 1 cut(s) 813
MseI TTAA 3 cut(s) 723, 828, 1015
Msp20I TGGCCA 1 cut(s) 813
MspA1I CMGCKG 1 cut(s) 451
MspI CCGG 3 cut(s) 110, 206, 583
MspR9I CCNGG 4 cut(s) 201, 515, 584, 688
MvaI CCWGG 3 cut(s) 201, 515, 688
MwoI GCNNNNNNNGC 4 cut(s) 289, 810, 846, 882
NaeI GCCGGC 1 cut(s) 111
NciI CCSGG 1 cut(s) 584
NdeII GATC 6 cut(s) 145, 436, 493, 646, 705, 730
NgoMIV GCCGGC 1 cut(s) 109
NlaIII CATG 5 cut(s) 185, 388, 706, 907, 1003
NlaIV GGNNCC 1 cut(s) 476
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 2 cut(s) 227, 773
NspI RCATGY 1 cut(s) 907
PaeI GCATGC 1 cut(s) 907
PaeR7I CTCGAG 1 cut(s) 440
PceI AGGCCT 1 cut(s) 881
PciSI GCTCTTC 1 cut(s) 12
PdiI GCCGGC 1 cut(s) 111
PfeI GAWTC 1 cut(s) 266
PflFI GACNNNGTC 1 cut(s) 521
PfoI TCCNGGA 2 cut(s) 513, 582
PkrI GCNGC 4 cut(s) 214, 453, 532, 806
PleI GAGTC 2 cut(s) 395, 663
PpsI GAGTC 2 cut(s) 395, 663
Psp6I CCWGG 3 cut(s) 199, 513, 686
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 3 cut(s) 199, 513, 686
PspN4I GGNNCC 1 cut(s) 476
PspPI GGNCC 2 cut(s) 203, 523
PstI CTGCAG 1 cut(s) 887
PsyI GACNNNGTC 1 cut(s) 521
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 723, 828, 1015
SatI GCNGC 4 cut(s) 213, 452, 531, 805
Sau3AI GATC 6 cut(s) 145, 436, 493, 646, 705, 730
Sau96I GGNCC 2 cut(s) 203, 523
ScaI AGTACT 1 cut(s) 570
SchI GAGTC 2 cut(s) 395, 664
ScrFI CCNGG 4 cut(s) 201, 515, 584, 688
SfaNI GCATC 3 cut(s) 166, 618, 934
SfcI CTRYAG 2 cut(s) 393, 883
Sfr274I CTCGAG 1 cut(s) 440
SgrAI CRCCGGYG 1 cut(s) 109
SinI GGWCC 2 cut(s) 203, 523
SlaI CTCGAG 1 cut(s) 440
SmlI CTYRAG 3 cut(s) 284, 440, 716
SmoI CTYRAG 3 cut(s) 284, 440, 716
SphI GCATGC 1 cut(s) 907
Sse9I AATT 3 cut(s) 79, 738, 823
SseBI AGGCCT 1 cut(s) 881
SsiI CCGC 3 cut(s) 113, 161, 451
StuI AGGCCT 1 cut(s) 881
StyD4I CCNGG 4 cut(s) 199, 513, 582, 686
TaaI ACNGT 2 cut(s) 98, 779
TaiI ACGT 1 cut(s) 875
TaqI TCGA 5 cut(s) 93, 126, 441, 496, 603
TasI AATT 3 cut(s) 79, 738, 823
TatI WGTACW 1 cut(s) 568
TauI GCSGC 1 cut(s) 454
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 3 cut(s) 723, 828, 1015
Tru9I TTAA 3 cut(s) 723, 828, 1015
TscAI CASTG 2 cut(s) 232, 784
TseFI GTSAC 2 cut(s) 227, 773
TseI GCWGC 3 cut(s) 212, 530, 804
Tsp45I GTSAC 2 cut(s) 227, 773
TspDTI ATGAA 3 cut(s) 170, 711, 909
TspRI CASTG 2 cut(s) 232, 784
Tth111I GACNNNGTC 1 cut(s) 521
VpaK11BI GGWCC 2 cut(s) 203, 523
XagI CCTNNNNNAGG 1 cut(s) 518
XapI RAATTY 1 cut(s) 79
XceI RCATGY 1 cut(s) 907
XhoI CTCGAG 1 cut(s) 440
XmiI GTMKAC 2 cut(s) 392, 1008
ZrmI AGTACT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.