Rorug06G0364400

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010150
Physical Location & Seq
Forward (+)
51190700 .. 51192596
1897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0364400.1

Sequence Viewer

Length: 933 bp
ATGGGGAGAGTAAAACTTCAGATGAAGAGAATTGAGAATACCGCTAACAGACAGATCACTTTCTCAAAGAGAAAAAACGGACTTATTAAAAAAGCTTATGAACTGTCTGTTCTCTGTGATGTTGATGTAGCTCTCATCATGTTTTCTCCGTCTGGGAGACTTAGTCTATTCTCTGGCAATAAAAGTATTGAAGACATTTTAGCACGATATATCAATTATTCAAGACATGAACGAGGACGTCTTCATAATGAAGAGATTCTTCGCAGCATTCTTAATAAGCTGAAGAGTGAAGTTAATCAAACGTTTCAAACAATTAGCCCAGTTAGTGCAGATTTTGATCATGTGCAGGAGATTCAACAAGAAATCCTATACTGTAAGTCACAAATGGAAGAAGTGGAAAATCAACTCAGAATATTTGAAGGTGATCTCTCTAAGATCACAACACTGCCCGATATTGAGTTACGGGAGCAGATCCTCGAGAAAACTCTTAATCGTCTATACATGCGCAAGCAAGTTCTAGGAGTGTGTAATTCTTCCTCTGCTATAGCAGTACAACCACAGATTCATCTTCCTCCAAATGCAGATGAACTTGTGATTGCAGCAGGAAATAGTACTACTCAACCCACTTATCTGGACCCTCAAGTTGAGATCCTAAATTTCTTGAGTGCAAATGGCTTTCTTCCCCTCAGTAGTGATTCAGCGCCCCAACCAATTGCTGTAGTAGCACCAATGTTACCCCTTGATAGCCAAAACACGAATCTTGATGATGACATGATCAATCTAGCAAATGGTTTTGAGGGTGAAAATAACAATAGGAGATCAGTAGTAGCGCAAGTACAACAAGAACATCTTGAAATCGAACAAGGCACTGATGTCAACTTGATTCCATGGAATCAGTTGTATCCTACAGGTACAACTTTGATGTGCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

310

Amino Acids

34.97

Weight (kDa)

5.18

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 55 2.7e-25 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 241
Acc16I TGCGCA 1 cut(s) 506
AciI CCGC 1 cut(s) 42
AclI AACGTT 1 cut(s) 302
AclWI GGATC 2 cut(s) 466, 643
AcsI RAATTY 1 cut(s) 655
AcuI CTGAAG 1 cut(s) 302
AcyI GRCGYC 1 cut(s) 238
AfaI GTAC 4 cut(s) 552, 613, 837, 913
AgsI TTSAA 6 cut(s) 191, 222, 308, 356, 419, 854
AluBI AGCT 3 cut(s) 95, 131, 280
AluI AGCT 3 cut(s) 95, 131, 280
Alw26I GTCTC 1 cut(s) 151
AlwI GGATC 2 cut(s) 466, 643
Ama87I CYCGRG 1 cut(s) 476
ApeKI GCWGC 2 cut(s) 264, 599
ApoI RAATTY 1 cut(s) 655
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 3 cut(s) 507, 703, 832
AspS9I GGNCC 1 cut(s) 634
AsuHPI GGTGA 2 cut(s) 434, 812
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 1 cut(s) 634
BbsI GAAGAC 2 cut(s) 198, 233
BbvI GCAGC 2 cut(s) 276, 611
BciVI GTATCC 1 cut(s) 912
BclI TGATCA 2 cut(s) 337, 774
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 2 cut(s) 518, 782
BfmI CTRYAG 3 cut(s) 543, 717, 906
BfoI RGCGCY 1 cut(s) 704
BfuI GTATCC 1 cut(s) 912
BisI GCNGC 2 cut(s) 265, 600
BlsI GCNGC 2 cut(s) 266, 601
BmcAI AGTACT 1 cut(s) 613
Bme18I GGWCC 1 cut(s) 634
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 1 cut(s) 634
BmiI GGNNCC 1 cut(s) 636
BmrI ACTGGG 1 cut(s) 314
BmuI ACTGGG 1 cut(s) 314
BpiI GAAGAC 2 cut(s) 198, 233
BpuEI CTTGAG 2 cut(s) 624, 682
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 238
BsaJI CCNNGG 1 cut(s) 887
BsaXI ACNNNNNCTCC 2 cut(s) 148, 178
Bse1I ACTGG 1 cut(s) 320
Bse8I GATNNNNATC 1 cut(s) 336
BseDI CCNNGG 1 cut(s) 887
BseJI GATNNNNATC 1 cut(s) 336
BseMII CTCAG 2 cut(s) 421, 700
BseNI ACTGG 1 cut(s) 320
BseXI GCAGC 2 cut(s) 276, 611
BsgI GTGCAG 2 cut(s) 348, 365
BsiHKCI CYCGRG 1 cut(s) 476
BsmAI GTCTC 1 cut(s) 151
BsmI GAATGC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 476
Bsp143I GATC 8 cut(s) 54, 337, 424, 435, 471, 648, 774, 818
Bsp19I CCATGG 1 cut(s) 887
BspACI CCGC 1 cut(s) 42
BspCNI CTCAG 2 cut(s) 420, 699
BspLI GGNNCC 1 cut(s) 636
BspPI GGATC 2 cut(s) 466, 643
BsrI ACTGG 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 887
BssMI GATC 8 cut(s) 54, 337, 424, 435, 471, 648, 774, 818
BssNI GRCGYC 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 887
Bst4CI ACNGT 2 cut(s) 105, 374
Bst6I CTCTTC 3 cut(s) 20, 246, 278
BstACI GRCGYC 1 cut(s) 238
BstC8I GCNNGC 1 cut(s) 509
BstDEI CTNAG 4 cut(s) 161, 407, 432, 686
BstDSI CCRYGG 1 cut(s) 887
BstH2I RGCGCY 1 cut(s) 704
BstHHI GCGC 3 cut(s) 507, 703, 832
BstKTI GATC 8 cut(s) 57, 340, 427, 438, 474, 651, 777, 821
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 8 cut(s) 54, 337, 424, 435, 471, 648, 774, 818
BstMWI GCNNNNNNNGC 1 cut(s) 722
BstNSI RCATGY 1 cut(s) 505
BstSFI CTRYAG 3 cut(s) 543, 717, 906
BstV1I GCAGC 2 cut(s) 276, 611
BstV2I GAAGAC 2 cut(s) 198, 233
BstX2I RGATCY 2 cut(s) 471, 648
BstXI CCANNNNNNTGG 1 cut(s) 631
BstYI RGATCY 2 cut(s) 471, 648
BsuI GTATCC 1 cut(s) 912
BtgI CCRYGG 1 cut(s) 887
BtsI GCAGTG 1 cut(s) 443
BtsIMutI CAGTG 2 cut(s) 443, 867
Cac8I GCNNGC 1 cut(s) 509
CfoI GCGC 3 cut(s) 507, 703, 832
Cfr13I GGNCC 1 cut(s) 634
Csp6I GTAC 4 cut(s) 551, 612, 836, 912
CviAII CATG 6 cut(s) 139, 227, 341, 502, 772, 888
CviJI RGCY 6 cut(s) 95, 131, 280, 318, 675, 747
CviKI_1 RGCY 6 cut(s) 95, 131, 280, 318, 675, 747
CviQI GTAC 4 cut(s) 551, 612, 836, 912
DdeI CTNAG 4 cut(s) 161, 407, 432, 686
DpnI GATC 8 cut(s) 56, 339, 426, 437, 473, 650, 776, 820
DpnII GATC 8 cut(s) 54, 337, 424, 435, 471, 648, 774, 818
Eam1104I CTCTTC 3 cut(s) 20, 246, 278
EarI CTCTTC 3 cut(s) 20, 246, 278
Eco130I CCWWGG 1 cut(s) 887
Eco47I GGWCC 1 cut(s) 634
Eco57I CTGAAG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 887
ErhI CCWWGG 1 cut(s) 887
FaeI CATG 6 cut(s) 142, 230, 344, 505, 775, 891
FalI AAGNNNNNCTT 4 cut(s) 243, 275, 834, 866
FatI CATG 6 cut(s) 138, 226, 340, 501, 771, 887
FbaI TGATCA 2 cut(s) 337, 774
Fnu4HI GCNGC 2 cut(s) 265, 600
Fsp4HI GCNGC 2 cut(s) 265, 600
FspBI CTAG 2 cut(s) 518, 782
FspI TGCGCA 1 cut(s) 506
GlaI GCGC 3 cut(s) 506, 702, 831
GluI GCNGC 2 cut(s) 265, 600
HaeII RGCGCY 1 cut(s) 704
HhaI GCGC 3 cut(s) 507, 703, 832
Hin1I GRCGYC 1 cut(s) 238
Hin1II CATG 6 cut(s) 142, 230, 344, 505, 775, 891
Hin6I GCGC 3 cut(s) 505, 701, 830
HinP1I GCGC 3 cut(s) 505, 701, 830
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HindIII AAGCTT 1 cut(s) 93
HinfI GANTC 7 cut(s) 256, 352, 562, 695, 757, 883, 892
HphI GGTGA 2 cut(s) 434, 812
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 2 cut(s) 21, 410
Hpy188III TCNNGA 6 cut(s) 222, 478, 632, 661, 761, 851
Hpy8I GTNNAC 1 cut(s) 877
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 2 cut(s) 105, 374
HpyCH4IV ACGT 2 cut(s) 238, 302
HpyCH4V TGCA 6 cut(s) 329, 346, 581, 599, 668, 927
HpyF10VI GCNNNNNNNGC 1 cut(s) 722
HpyF3I CTNAG 4 cut(s) 161, 407, 432, 686
HpySE526I ACGT 2 cut(s) 238, 302
Hsp92I GRCGYC 1 cut(s) 238
Hsp92II CATG 6 cut(s) 142, 230, 344, 505, 775, 891
HspAI GCGC 3 cut(s) 505, 701, 830
Ksp22I TGATCA 2 cut(s) 337, 774
Kzo9I GATC 8 cut(s) 54, 337, 424, 435, 471, 648, 774, 818
LmnI GCTCC 1 cut(s) 466
LpnPI CCDG 7 cut(s) 138, 159, 332, 333, 588, 617, 894
Lsp1109I GCAGC 2 cut(s) 276, 611
MaeI CTAG 2 cut(s) 518, 782
MaeII ACGT 2 cut(s) 238, 302
MaeIII GTNAC 3 cut(s) 378, 459, 732
MalI GATC 8 cut(s) 56, 339, 426, 437, 473, 650, 776, 820
MboI GATC 8 cut(s) 54, 337, 424, 435, 471, 648, 774, 818
MfeI CAATTG 1 cut(s) 711
MflI RGATCY 2 cut(s) 471, 648
MluCI AATT 6 cut(s) 30, 214, 312, 529, 655, 711
MnlI CCTC 7 cut(s) 227, 485, 547, 582, 648, 695, 790
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 4 cut(s) 87, 273, 294, 489
MunI CAATTG 1 cut(s) 711
Mva1269I GAATGC 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 722
NcoI CCATGG 1 cut(s) 887
NdeII GATC 8 cut(s) 54, 337, 424, 435, 471, 648, 774, 818
NlaIII CATG 6 cut(s) 142, 230, 344, 505, 775, 891
NlaIV GGNNCC 1 cut(s) 636
NmuCI GTSAC 1 cut(s) 378
NsbI TGCGCA 1 cut(s) 506
NspI RCATGY 1 cut(s) 505
PaeR7I CTCGAG 1 cut(s) 476
PctI GAATGC 1 cut(s) 267
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 7 cut(s) 256, 352, 562, 695, 757, 883, 892
PflFI GACNNNGTC 1 cut(s) 162
PkrI GCNGC 2 cut(s) 266, 601
Psp1406I AACGTT 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 636
PspPI GGNCC 1 cut(s) 634
PsuI RGATCY 2 cut(s) 471, 648
PsyI GACNNNGTC 1 cut(s) 162
RsaI GTAC 4 cut(s) 552, 613, 837, 913
RsaNI GTAC 4 cut(s) 551, 612, 836, 912
SaqAI TTAA 4 cut(s) 87, 273, 294, 489
SatI GCNGC 2 cut(s) 265, 600
Sau3AI GATC 8 cut(s) 54, 337, 424, 435, 471, 648, 774, 818
Sau96I GGNCC 1 cut(s) 634
ScaI AGTACT 1 cut(s) 613
SetI ASST 7 cut(s) 97, 133, 241, 282, 305, 424, 913
SfcI CTRYAG 3 cut(s) 543, 717, 906
Sfr274I CTCGAG 1 cut(s) 476
SinI GGWCC 1 cut(s) 634
SlaI CTCGAG 1 cut(s) 476
SmlI CTYRAG 3 cut(s) 476, 639, 661
SmoI CTYRAG 3 cut(s) 476, 639, 661
Sse9I AATT 6 cut(s) 30, 214, 312, 529, 655, 711
SsiI CCGC 1 cut(s) 42
SspI AATATT 1 cut(s) 414
SspMI CTAG 2 cut(s) 518, 782
StyI CCWWGG 1 cut(s) 887
TaaI ACNGT 2 cut(s) 105, 374
TaiI ACGT 2 cut(s) 241, 305
TaqI TCGA 2 cut(s) 477, 858
TasI AATT 6 cut(s) 30, 214, 312, 529, 655, 711
TatI WGTACW 3 cut(s) 550, 611, 835
TfiI GAWTC 7 cut(s) 256, 352, 562, 695, 757, 883, 892
Tru1I TTAA 4 cut(s) 87, 273, 294, 489
Tru9I TTAA 4 cut(s) 87, 273, 294, 489
TscAI CASTG 2 cut(s) 450, 874
TseFI GTSAC 1 cut(s) 378
TseI GCWGC 2 cut(s) 264, 599
Tsp45I GTSAC 1 cut(s) 378
TspDTI ATGAA 7 cut(s) 38, 114, 233, 243, 264, 554, 600
TspGWI ACGGA 2 cut(s) 93, 138
TspRI CASTG 2 cut(s) 450, 874
Tth111I GACNNNGTC 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 634
XapI RAATTY 1 cut(s) 655
XceI RCATGY 1 cut(s) 505
XhoI CTCGAG 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 2 cut(s) 518, 782
ZraI GACGTC 1 cut(s) 239
ZrmI AGTACT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.