MD08G1098300.v1.1

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010150
Physical Location & Seq
Reverse (-)
8289357 .. 8292565
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1098300.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGCTGTTCCCCAGCACCATTTCCAACAGCACTACCCACCCCAACAACAGCAGCAACAATCCAAGTCTTTCAGAAATTTGTACACAATCGACGGCCAGATGTCGCCGGCGGTGGCGTATTACGACCCCGGCAATTTACACGATCACCCCCAGCAGCAGCATCCTCCTTACGTTCCGCCCTTTCATGTAGTAGGATTCGCGCCCGGTCCGGTGGCTGCAACGGATGGCAGCGATAATGGTGCCGATTTGCAGTGTCATTATGGGTTCGAATCCAAAAGGAAAAAGCTAAAGGAACAGGATTTCCTGGAGAACAACTCACAAATATCGTCCATTGATTTCCTGCAACCGCAATCGGTCTCCACAGGCTTGGGTTTGTCCCTTGACAATACTCGAATGGGTTCTACAGGGGACTCATCGTTGCTGTCACTTATCAGCGATGACATTGATCATGAGCTACAACGACAGGATGCGGAGATTGATAGATTCCTCAAGGCCCAGGGTGACCGACTGCGGCAAACTATCATGGACAAGGTCCAAGCCACACAACTTCAGACCCTCTCGGCTGTGGAAGACAAAGTTCATCGGAAGCTGCGAGAGAAAGAAGCAGAAGTGGAGAGCATTAACGAGAAGAATATGGAGCTTGAGGAGCGAATGGAACAGTTGACTGTTGAAGCAGGTGCTTGGCAACAGCTAGCCAGGCACAATGAAAACATGATCTCAACTCTAAGGTTTAATCTTCAGCATATATATGCTCAAAGTAGAGATAGTAAAGAAGGATGTGGCGACAGTGAGGTAGATGATACAGCTTCTTGCTGCAATGGCCGTTCGATCAATTTGGAAATGTTTTGCAAGGAGAATAATGACGGGAAAGAGATGATGACTTGCAAGGCCTGCCGAGTCAATGAAGTGTGCATGCTTCTATTACCTTGTAAGCATCTTTGCCTGTGCAAAGATTGTGAAAGTAAGCTCAGCATCTGCCCTCTCTGTCAGTCCTCTAAGTTTATCAGCATGGAGGTATATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

342

Amino Acids

38.62

Weight (kDa)

5.31

Isoelectric Point (pI)

56.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 293 - 331 1.6e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 665
AccB1I GGYRCC 1 cut(s) 239
AccII CGCG 1 cut(s) 200
AciI CCGC 5 cut(s) 110, 176, 347, 470, 511
AcoI YGGCCR 2 cut(s) 94, 820
AcsI RAATTY 1 cut(s) 76
AcuI CTGAAG 2 cut(s) 533, 722
AfaI GTAC 1 cut(s) 83
AgsI TTSAA 1 cut(s) 671
AjnI CCWGG 3 cut(s) 303, 495, 695
AloI GAACNNNNNNTCC 2 cut(s) 285, 317
AluBI AGCT 7 cut(s) 286, 454, 589, 640, 691, 806, 967
AluI AGCT 7 cut(s) 286, 454, 589, 640, 691, 806, 967
Alw26I GTCTC 1 cut(s) 361
AlwNI CAGNNNCTG 1 cut(s) 975
AoxI GGCC 4 cut(s) 94, 492, 820, 888
ApeKI GCWGC 7 cut(s) 52, 154, 157, 215, 228, 589, 813
ApoI RAATTY 1 cut(s) 76
Asp700I GAANNNNTTC 1 cut(s) 397
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 3 cut(s) 206, 493, 532
AsuC2I CCSGG 2 cut(s) 129, 204
AsuHPI GGTGA 2 cut(s) 137, 512
AsuII TTCGAA 1 cut(s) 267
AsuNHI GCTAGC 1 cut(s) 691
AvaII GGWCC 2 cut(s) 206, 532
BanI GGYRCC 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 576
BbvI GCAGC 7 cut(s) 64, 166, 169, 202, 240, 576, 800
BccI CCATC 1 cut(s) 218
BceAI ACGGC 2 cut(s) 109, 807
BcgI CGANNNNNNTGC 2 cut(s) 221, 255
BciT130I CCWGG 3 cut(s) 305, 497, 697
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 2 cut(s) 129, 204
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 1 cut(s) 692
BfmI CTRYAG 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 665
BisI GCNGC 8 cut(s) 53, 155, 158, 216, 229, 512, 590, 814
BlpI GCTNAGC 1 cut(s) 968
BlsI GCNGC 8 cut(s) 54, 156, 159, 217, 230, 513, 591, 815
Bme1390I CCNGG 5 cut(s) 129, 204, 305, 497, 697
Bme18I GGWCC 2 cut(s) 206, 532
BmgT120I GGNCC 3 cut(s) 206, 493, 532
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 5 cut(s) 129, 204, 305, 497, 697
BmsI GCATC 4 cut(s) 169, 457, 943, 981
BmtI GCTAGC 1 cut(s) 695
BpiI GAAGAC 1 cut(s) 576
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BpmI CTGGAG 1 cut(s) 326
Bpu1102I GCTNAGC 1 cut(s) 968
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 2 cut(s) 473, 662
BpuMI CCSGG 2 cut(s) 129, 204
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 3 cut(s) 127, 495, 496
BsaWI WCCGGW 1 cut(s) 208
Bse118I RCCGGY 1 cut(s) 106
Bse3DI GCAATG 1 cut(s) 823
BseBI CCWGG 3 cut(s) 305, 497, 697
BseDI CCNNGG 3 cut(s) 127, 495, 496
BseGI GGATG 4 cut(s) 160, 229, 472, 782
BseMI GCAATG 1 cut(s) 823
BseMII CTCAG 1 cut(s) 982
BseRI GAGGAG 1 cut(s) 659
BseXI GCAGC 7 cut(s) 64, 166, 169, 202, 240, 576, 800
BseYI CCCAGC 2 cut(s) 12, 150
Bsh1236I CGCG 1 cut(s) 200
BshFI GGCC 4 cut(s) 96, 494, 822, 890
BshNI GGYRCC 1 cut(s) 239
BsiSI CCGG 4 cut(s) 107, 129, 204, 209
BslFI GGGAC 2 cut(s) 361, 422
BsmAI GTCTC 1 cut(s) 361
BsmFI GGGAC 2 cut(s) 361, 422
BsnI GGCC 4 cut(s) 96, 494, 822, 890
Bso31I GGTCTC 1 cut(s) 361
Bsp119I TTCGAA 1 cut(s) 267
Bsp1407I TGTACA 1 cut(s) 81
Bsp143I GATC 4 cut(s) 142, 445, 714, 828
Bsp1720I GCTNAGC 1 cut(s) 968
BspACI CCGC 5 cut(s) 110, 176, 347, 470, 511
BspANI GGCC 4 cut(s) 96, 494, 822, 890
BspCNI CTCAG 1 cut(s) 981
BspFNI CGCG 1 cut(s) 200
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 665
BspOI GCTAGC 1 cut(s) 695
BspT104I TTCGAA 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 823
BsrFI RCCGGY 1 cut(s) 106
BsrGI TGTACA 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 3 cut(s) 127, 495, 496
BssMI GATC 4 cut(s) 142, 445, 714, 828
Bst2UI CCWGG 3 cut(s) 305, 497, 697
Bst4CI ACNGT 3 cut(s) 660, 667, 788
BstAPI GCANNNNNTGC 1 cut(s) 891
BstAUI TGTACA 1 cut(s) 81
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 4 cut(s) 108, 693, 892, 914
BstDEI CTNAG 3 cut(s) 725, 968, 996
BstEII GGTNACC 1 cut(s) 500
BstF5I GGATG 4 cut(s) 160, 229, 472, 782
BstFNI CGCG 1 cut(s) 200
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 4 cut(s) 145, 448, 717, 831
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 4 cut(s) 142, 445, 714, 828
BstMWI GCNNNNNNNGC 4 cut(s) 646, 697, 819, 891
BstNI CCWGG 3 cut(s) 305, 497, 697
BstNSI RCATGY 1 cut(s) 916
BstPI GGTNACC 1 cut(s) 500
BstSCI CCNGG 5 cut(s) 127, 202, 303, 495, 695
BstSFI CTRYAG 1 cut(s) 402
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 7 cut(s) 64, 166, 169, 202, 240, 576, 800
BstV2I GAAGAC 1 cut(s) 576
BstXI CCANNNNNNTGG 1 cut(s) 367
BsuRI GGCC 4 cut(s) 96, 494, 822, 890
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 4 cut(s) 160, 229, 472, 782
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 2 cut(s) 257, 793
BveI ACCTGC 1 cut(s) 665
Cac8I GCNNGC 4 cut(s) 108, 693, 892, 914
CaiI CAGNNNCTG 1 cut(s) 975
CciI TCATGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 3 cut(s) 206, 493, 532
CpoI CGGWCCG 1 cut(s) 206
Csp6I GTAC 1 cut(s) 82
CspI CGGWCCG 1 cut(s) 206
CviAII CATG 6 cut(s) 185, 449, 523, 712, 913, 1009
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 3 cut(s) 725, 968, 996
DpnI GATC 4 cut(s) 144, 447, 716, 830
DpnII GATC 4 cut(s) 142, 445, 714, 828
EaeI YGGCCR 2 cut(s) 94, 820
EciI GGCGGA 1 cut(s) 165
Eco147I AGGCCT 1 cut(s) 890
Eco31I GGTCTC 1 cut(s) 361
Eco47I GGWCC 2 cut(s) 206, 532
Eco57I CTGAAG 2 cut(s) 533, 722
Eco91I GGTNACC 1 cut(s) 500
EcoO65I GGTNACC 1 cut(s) 500
EcoRII CCWGG 3 cut(s) 303, 495, 695
FaeI CATG 6 cut(s) 188, 452, 526, 715, 916, 1012
FaqI GGGAC 2 cut(s) 361, 422
FatI CATG 6 cut(s) 184, 448, 522, 711, 912, 1008
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 8 cut(s) 53, 155, 158, 216, 229, 512, 590, 814
FokI GGATG 4 cut(s) 147, 236, 479, 789
Fsp4HI GCNGC 8 cut(s) 53, 155, 158, 216, 229, 512, 590, 814
FspBI CTAG 1 cut(s) 692
GlaI GCGC 1 cut(s) 201
GluI GCNGC 8 cut(s) 53, 155, 158, 216, 229, 512, 590, 814
GsaI CCCAGC 2 cut(s) 16, 154
GsuI CTGGAG 1 cut(s) 326
HaeIII GGCC 4 cut(s) 96, 494, 822, 890
HapII CCGG 4 cut(s) 107, 129, 204, 209
HhaI GCGC 1 cut(s) 202
Hin1II CATG 6 cut(s) 188, 452, 526, 715, 916, 1012
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HinfI GANTC 5 cut(s) 195, 269, 410, 483, 897
HpaII CCGG 4 cut(s) 107, 129, 204, 209
HphI GGTGA 2 cut(s) 137, 512
Hpy166II GTNNAC 2 cut(s) 84, 663
Hpy188I TCNGA 3 cut(s) 74, 552, 585
Hpy188III TCNNGA 1 cut(s) 449
Hpy8I GTNNAC 2 cut(s) 84, 663
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 1 cut(s) 767
HpyCH4III ACNGT 3 cut(s) 660, 667, 788
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 8 cut(s) 218, 250, 343, 816, 849, 885, 912, 948
HpyF10VI GCNNNNNNNGC 4 cut(s) 646, 697, 819, 891
HpyF3I CTNAG 3 cut(s) 725, 968, 996
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 6 cut(s) 188, 452, 526, 715, 916, 1012
HspAI GCGC 1 cut(s) 200
KroI GCCGGC 1 cut(s) 106
KroNI GCCGGC 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 4 cut(s) 142, 445, 714, 828
LmnI GCTCC 2 cut(s) 637, 646
Lsp1109I GCAGC 7 cut(s) 64, 166, 169, 202, 240, 576, 800
LweI GCATC 4 cut(s) 169, 457, 943, 981
MaeI CTAG 1 cut(s) 692
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 423, 500
MalI GATC 4 cut(s) 144, 447, 716, 830
MboI GATC 4 cut(s) 142, 445, 714, 828
MboII GAAGA 3 cut(s) 581, 640, 728
MluCI AATT 3 cut(s) 76, 133, 832
MlyI GAGTC 2 cut(s) 404, 906
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 8 cut(s) 174, 497, 566, 637, 784, 990, 1003, 1006
MreI CGCCGGCG 1 cut(s) 106
MroNI GCCGGC 1 cut(s) 106
MroXI GAANNNNTTC 1 cut(s) 397
MseI TTAA 2 cut(s) 621, 732
MslI CAYNNNNRTG 1 cut(s) 747
MspI CCGG 4 cut(s) 107, 129, 204, 209
MspR9I CCNGG 5 cut(s) 129, 204, 305, 497, 697
MvaI CCWGG 3 cut(s) 305, 497, 697
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 4 cut(s) 646, 697, 819, 891
NaeI GCCGGC 1 cut(s) 108
NciI CCSGG 2 cut(s) 129, 204
NdeII GATC 4 cut(s) 142, 445, 714, 828
NgoMIV GCCGGC 1 cut(s) 106
NheI GCTAGC 1 cut(s) 691
NlaIII CATG 6 cut(s) 188, 452, 526, 715, 916, 1012
NlaIV GGNNCC 1 cut(s) 241
NmeAIII GCCGAG 2 cut(s) 539, 920
NmuCI GTSAC 2 cut(s) 423, 500
NspI RCATGY 1 cut(s) 916
NspV TTCGAA 1 cut(s) 267
PaeI GCATGC 1 cut(s) 916
PagI TCATGA 1 cut(s) 448
PaqCI CACCTGC 1 cut(s) 665
PasI CCCWGGG 1 cut(s) 496
PceI AGGCCT 1 cut(s) 890
PcsI WCGNNNNNNNCGW 1 cut(s) 589
PdiI GCCGGC 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 397
PfeI GAWTC 3 cut(s) 195, 269, 483
PflFI GACNNNGTC 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 303
PkrI GCNGC 8 cut(s) 54, 156, 159, 217, 230, 513, 591, 815
PleI GAGTC 2 cut(s) 404, 905
PpsI GAGTC 2 cut(s) 404, 905
Psp6I CCWGG 3 cut(s) 303, 495, 695
PspEI GGTNACC 1 cut(s) 500
PspFI CCCAGC 2 cut(s) 12, 150
PspGI CCWGG 3 cut(s) 303, 495, 695
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 3 cut(s) 206, 493, 532
PstNI CAGNNNCTG 1 cut(s) 975
PsyI GACNNNGTC 1 cut(s) 530
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
RseI CAYNNNNRTG 1 cut(s) 747
Rsr2I CGGWCCG 1 cut(s) 206
RsrII CGGWCCG 1 cut(s) 206
SaqAI TTAA 2 cut(s) 621, 732
SatI GCNGC 8 cut(s) 53, 155, 158, 216, 229, 512, 590, 814
Sau3AI GATC 4 cut(s) 142, 445, 714, 828
Sau96I GGNCC 3 cut(s) 206, 493, 532
SchI GAGTC 2 cut(s) 404, 906
ScrFI CCNGG 5 cut(s) 129, 204, 305, 497, 697
SfaNI GCATC 4 cut(s) 169, 457, 943, 981
SfcI CTRYAG 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 267
SgrAI CRCCGGYG 1 cut(s) 106
SinI GGWCC 2 cut(s) 206, 532
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 2 cut(s) 488, 641
SmoI CTYRAG 2 cut(s) 488, 641
SphI GCATGC 1 cut(s) 916
Sse9I AATT 3 cut(s) 76, 133, 832
SseBI AGGCCT 1 cut(s) 890
SsiI CCGC 5 cut(s) 110, 176, 347, 470, 511
SspMI CTAG 1 cut(s) 692
StuI AGGCCT 1 cut(s) 890
StyD4I CCNGG 5 cut(s) 127, 202, 303, 495, 695
TaaI ACNGT 3 cut(s) 660, 667, 788
TaiI ACGT 1 cut(s) 174
TaqI TCGA 4 cut(s) 90, 267, 391, 827
TaqII GACCGA 2 cut(s) 343, 519
TasI AATT 3 cut(s) 76, 133, 832
TatI WGTACW 1 cut(s) 81
TauI GCSGC 1 cut(s) 514
TfiI GAWTC 3 cut(s) 195, 269, 483
Tru1I TTAA 2 cut(s) 621, 732
Tru9I TTAA 2 cut(s) 621, 732
TscAI CASTG 2 cut(s) 257, 793
TseFI GTSAC 2 cut(s) 423, 500
TseI GCWGC 7 cut(s) 52, 154, 157, 215, 228, 589, 813
Tsp45I GTSAC 2 cut(s) 423, 500
TspDTI ATGAA 4 cut(s) 173, 569, 720, 918
TspGWI ACGGA 1 cut(s) 236
TspRI CASTG 2 cut(s) 257, 793
Tth111I GACNNNGTC 1 cut(s) 530
VpaK11BI GGWCC 2 cut(s) 206, 532
XapI RAATTY 1 cut(s) 76
XceI RCATGY 1 cut(s) 916
XmnI GAANNNNTTC 1 cut(s) 397
XspI CTAG 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.