Rw6G041290

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010150
Physical Location & Seq
Reverse (-)
65016609 .. 65019715
3107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041290.1

Sequence Viewer

Length: 1020 bp
ATGGCTCTTCCCCAACACCACTTTCAACAACACTATCCACCCCAACAACAACAACAGCAGCAGCAATCCAAGTCATTCAGAAATTTATACACAATCGACGGTCAGATGTCGCCGGCGGTGGCCTATTTCGACCCCAACGCCTTACAAGATCACTCCCAGCATCCGCCTTATGTTCCACCCTTTCATGTAGTAGGGTTTGCGCCTGGTCCGGTGGCTGCAACAGATGGCAGTGACAATGGTGCTGATTTTCAATGGAACTATGGCTTAGAATCCAAGAAGAAAAGGCTCAAGGAGCAGGATTTTTTGGAGAACAACTCGCAAATGTCTTCGGTTCTGCAACAGCAATCAGTCTCAACGGGCTTGGGTCTGTCCCTTGACAATAATCGCATGGCGTCTACAGGGGACTCGGCTTTGCTGTCTCTCATTGGCGAGGATGTTGATCTCGAGATACAGCGGCAAGATGTTGAGATTGATAGGTTCCTCAAAGCCCAGAGTGATCGATTGCGACAAAATATCCTGGACAAGGTCCAAGCTGCCCAACTTCAAACTCTCTCAGTTGTAGAAGAAAAAGTATTCCAGAAGCTCCGGGAAAAAGAAGCAGAAGTTGAGAGCATCAACAAGAAGAATATGGAGCTTGAAGAAAGAATGGATCAGTTGAGTCTTGAAGCAAGGATTTGGCAACATCAAGCCAGGAACAATGAACACATGATCACAGCTCTCAAGGTTAATCTTGATCAAGTAATTGCTCAAAGTAGAGACAGCAAGGAAGGATGTGGTGACAGTGAAATAGATGATACAGCTTCCTGCTGCAATGGCCATTCCATCAATTTTAATATGTTTGGCAAGGAGAGCAATGATGTGAAAGAGGTAATGACGTGCAAGGCCTGCAGAGTGAATGAAGTTTGCATGCTTCTATTACCCTGTAAGCATCTTTGCCTGTGTAAAGATTGCGAATGTAAGCTTAGCATTTGTCCTCTGTGTCAGTCCTCTAAGTTTATCGGCATGGAAGTATACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

339

Amino Acids

38.41

Weight (kDa)

5.26

Isoelectric Point (pI)

55.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 291 - 329 9.2e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 395, 1011
AciI CCGC 3 cut(s) 116, 164, 454
AclWI GGATC 1 cut(s) 657
AcoI YGGCCR 1 cut(s) 814
AcsI RAATTY 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 392
AfiI CCNNNNNNNGG 1 cut(s) 523
AgsI TTSAA 5 cut(s) 26, 251, 545, 638, 665
AjiI CACGTC 1 cut(s) 876
AjnI CCWGG 3 cut(s) 202, 516, 689
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AluBI AGCT 6 cut(s) 533, 583, 634, 716, 800, 961
AluI AGCT 6 cut(s) 533, 583, 634, 716, 800, 961
Alw26I GTCTC 3 cut(s) 355, 423, 750
AlwI GGATC 1 cut(s) 657
Ama87I CYCGRG 1 cut(s) 443
AoxI GGCC 3 cut(s) 120, 814, 882
ApeKI GCWGC 5 cut(s) 58, 61, 215, 533, 807
ApoI RAATTY 1 cut(s) 82
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 2 cut(s) 206, 526
AsuC2I CCSGG 1 cut(s) 587
AsuHPI GGTGA 1 cut(s) 788
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 2 cut(s) 206, 526
BalI TGGCCA 1 cut(s) 816
BbsI GAAGAC 1 cut(s) 318
BbvI GCAGC 5 cut(s) 70, 73, 202, 520, 794
BccI CCATC 2 cut(s) 218, 830
BciT130I CCWGG 3 cut(s) 204, 518, 691
BclI TGATCA 2 cut(s) 708, 733
BcnI CCSGG 1 cut(s) 587
BcoDI GTCTC 3 cut(s) 355, 423, 750
BfmI CTRYAG 2 cut(s) 396, 886
BisI GCNGC 6 cut(s) 59, 62, 216, 455, 534, 808
BlpI GCTNAGC 1 cut(s) 962
BlsI GCNGC 6 cut(s) 60, 63, 217, 456, 535, 809
Bme1390I CCNGG 4 cut(s) 204, 518, 587, 691
Bme18I GGWCC 2 cut(s) 206, 526
BmeT110I CYCGRG 1 cut(s) 443
BmgBI CACGTC 1 cut(s) 876
BmgT120I GGNCC 2 cut(s) 206, 526
BmiI GGNNCC 1 cut(s) 479
BmrFI CCNGG 4 cut(s) 204, 518, 587, 691
BmsI GCATC 3 cut(s) 169, 621, 937
BpiI GAAGAC 1 cut(s) 318
BplI GAGNNNNNCTC 2 cut(s) 299, 331
Bpu1102I GCTNAGC 1 cut(s) 962
BpuEI CTTGAG 2 cut(s) 272, 704
BpuMI CCSGG 1 cut(s) 587
Bsa29I ATCGAT 1 cut(s) 499
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 392
BsaWI WCCGGW 1 cut(s) 208
Bsc4I CCNNNNNNNGG 1 cut(s) 523
Bse118I RCCGGY 1 cut(s) 112
Bse3DI GCAATG 2 cut(s) 817, 859
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 3 cut(s) 204, 518, 691
BseCI ATCGAT 1 cut(s) 499
BseGI GGATG 3 cut(s) 160, 439, 776
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 1 cut(s) 523
BseMI GCAATG 2 cut(s) 817, 859
BseMII CTCAG 1 cut(s) 567
BseXI GCAGC 5 cut(s) 70, 73, 202, 520, 794
BseYI CCCAGC 1 cut(s) 156
BshFI GGCC 3 cut(s) 122, 816, 884
BshVI ATCGAT 1 cut(s) 499
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 3 cut(s) 113, 209, 586
BslFI GGGAC 2 cut(s) 355, 416
BslI CCNNNNNNNGG 1 cut(s) 523
BsmAI GTCTC 3 cut(s) 355, 423, 750
BsmFI GGGAC 2 cut(s) 355, 416
BsnI GGCC 3 cut(s) 122, 816, 884
BsoBI CYCGRG 1 cut(s) 443
Bsp143I GATC 6 cut(s) 148, 439, 496, 649, 708, 733
Bsp1720I GCTNAGC 1 cut(s) 962
BspACI CCGC 3 cut(s) 116, 164, 454
BspANI GGCC 3 cut(s) 122, 816, 884
BspCNI CTCAG 1 cut(s) 566
BspDI ATCGAT 1 cut(s) 499
BspLI GGNNCC 1 cut(s) 479
BspMAI CTGCAG 1 cut(s) 890
BspPI GGATC 1 cut(s) 657
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 817, 859
BsrFI RCCGGY 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 112
BssMI GATC 6 cut(s) 148, 439, 496, 649, 708, 733
BssNAI GTATAC 1 cut(s) 1012
BssNI GRCGYC 1 cut(s) 392
Bst1107I GTATAC 1 cut(s) 1012
Bst2UI CCWGG 3 cut(s) 204, 518, 691
Bst4CI ACNGT 2 cut(s) 101, 782
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 392
BstAPI GCANNNNNTGC 1 cut(s) 885
BstC8I GCNNGC 3 cut(s) 114, 886, 908
BstDEI CTNAG 4 cut(s) 265, 553, 962, 990
BstENI CCTNNNNNAGG 1 cut(s) 521
BstF5I GGATG 3 cut(s) 160, 439, 776
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 6 cut(s) 151, 442, 499, 652, 711, 736
BstMAI GTCTC 3 cut(s) 355, 423, 750
BstMBI GATC 6 cut(s) 148, 439, 496, 649, 708, 733
BstMWI GCNNNNNNNGC 4 cut(s) 292, 813, 849, 885
BstNI CCWGG 3 cut(s) 204, 518, 691
BstNSI RCATGY 1 cut(s) 910
BstSCI CCNGG 4 cut(s) 202, 516, 585, 689
BstSFI CTRYAG 2 cut(s) 396, 886
BstV1I GCAGC 5 cut(s) 70, 73, 202, 520, 794
BstV2I GAAGAC 1 cut(s) 318
BstZ17I GTATAC 1 cut(s) 1012
Bsu15I ATCGAT 1 cut(s) 499
BsuRI GGCC 3 cut(s) 122, 816, 884
BsuTUI ATCGAT 1 cut(s) 499
BtrI CACGTC 1 cut(s) 876
BtsCI GGATG 3 cut(s) 160, 439, 776
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 2 cut(s) 235, 787
Cac8I GCNNGC 3 cut(s) 114, 886, 908
CfoI GCGC 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 206, 526
ClaI ATCGAT 1 cut(s) 499
CseI GACGC 1 cut(s) 381
CviAII CATG 5 cut(s) 185, 388, 706, 907, 1003
DdeI CTNAG 4 cut(s) 265, 553, 962, 990
DpnI GATC 6 cut(s) 150, 441, 498, 651, 710, 735
DpnII GATC 6 cut(s) 148, 439, 496, 649, 708, 733
EaeI YGGCCR 1 cut(s) 814
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 884
Eco47I GGWCC 2 cut(s) 206, 526
Eco88I CYCGRG 1 cut(s) 443
EcoNI CCTNNNNNAGG 1 cut(s) 521
EcoRII CCWGG 3 cut(s) 202, 516, 689
FaeI CATG 5 cut(s) 188, 391, 709, 910, 1006
FaqI GGGAC 2 cut(s) 355, 416
FatI CATG 5 cut(s) 184, 387, 705, 906, 1002
FbaI TGATCA 2 cut(s) 708, 733
FblI GTMKAC 2 cut(s) 395, 1011
Fnu4HI GCNGC 6 cut(s) 59, 62, 216, 455, 534, 808
FokI GGATG 3 cut(s) 147, 446, 783
Fsp4HI GCNGC 6 cut(s) 59, 62, 216, 455, 534, 808
GlaI GCGC 1 cut(s) 201
GluI GCNGC 6 cut(s) 59, 62, 216, 455, 534, 808
GsaI CCCAGC 1 cut(s) 160
HaeIII GGCC 3 cut(s) 122, 816, 884
HapII CCGG 3 cut(s) 113, 209, 586
HgaI GACGC 1 cut(s) 381
HhaI GCGC 1 cut(s) 202
Hin1I GRCGYC 1 cut(s) 392
Hin1II CATG 5 cut(s) 188, 391, 709, 910, 1006
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HindIII AAGCTT 1 cut(s) 959
HinfI GANTC 3 cut(s) 269, 404, 658
HpaII CCGG 3 cut(s) 113, 209, 586
HphI GGTGA 1 cut(s) 788
Hpy166II GTNNAC 2 cut(s) 396, 1012
Hpy188I TCNGA 2 cut(s) 80, 105
Hpy188III TCNNGA 5 cut(s) 443, 445, 577, 662, 731
Hpy8I GTNNAC 2 cut(s) 396, 1012
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 761
HpyCH4III ACNGT 2 cut(s) 101, 782
HpyCH4IV ACGT 1 cut(s) 875
HpyCH4V TGCA 6 cut(s) 218, 337, 810, 879, 888, 906
HpyF10VI GCNNNNNNNGC 4 cut(s) 292, 813, 849, 885
HpyF3I CTNAG 4 cut(s) 265, 553, 962, 990
HpySE526I ACGT 1 cut(s) 875
Hsp92I GRCGYC 1 cut(s) 392
Hsp92II CATG 5 cut(s) 188, 391, 709, 910, 1006
HspAI GCGC 1 cut(s) 200
KroI GCCGGC 1 cut(s) 112
KroNI GCCGGC 1 cut(s) 114
Ksp22I TGATCA 2 cut(s) 708, 733
Kzo9I GATC 6 cut(s) 148, 439, 496, 649, 708, 733
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 292, 588, 631
Lsp1109I GCAGC 5 cut(s) 70, 73, 202, 520, 794
LweI GCATC 3 cut(s) 169, 621, 937
MaeII ACGT 1 cut(s) 875
MaeIII GTNAC 2 cut(s) 230, 776
MalI GATC 6 cut(s) 150, 441, 498, 651, 710, 735
MboI GATC 6 cut(s) 148, 439, 496, 649, 708, 733
MboII GAAGA 5 cut(s) 289, 318, 575, 634, 650
MlsI TGGCCA 1 cut(s) 816
MluCI AATT 3 cut(s) 82, 741, 826
MluNI TGGCCA 1 cut(s) 816
MlyI GAGTC 2 cut(s) 398, 667
MnlI CCTC 5 cut(s) 424, 491, 859, 984, 997
Mox20I TGGCCA 1 cut(s) 816
MreI CGCCGGCG 1 cut(s) 112
MroNI GCCGGC 1 cut(s) 112
MscI TGGCCA 1 cut(s) 816
MseI TTAA 3 cut(s) 726, 831, 1018
Msp20I TGGCCA 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 454
MspI CCGG 3 cut(s) 113, 209, 586
MspR9I CCNGG 4 cut(s) 204, 518, 587, 691
MvaI CCWGG 3 cut(s) 204, 518, 691
MwoI GCNNNNNNNGC 4 cut(s) 292, 813, 849, 885
NaeI GCCGGC 1 cut(s) 114
NciI CCSGG 1 cut(s) 587
NdeII GATC 6 cut(s) 148, 439, 496, 649, 708, 733
NgoMIV GCCGGC 1 cut(s) 112
NlaIII CATG 5 cut(s) 188, 391, 709, 910, 1006
NlaIV GGNNCC 1 cut(s) 479
NmeAIII GCCGAG 1 cut(s) 386
NmuCI GTSAC 2 cut(s) 230, 776
NspI RCATGY 1 cut(s) 910
PaeI GCATGC 1 cut(s) 910
PaeR7I CTCGAG 1 cut(s) 443
PceI AGGCCT 1 cut(s) 884
PciSI GCTCTTC 1 cut(s) 12
PdiI GCCGGC 1 cut(s) 114
PfeI GAWTC 1 cut(s) 269
PflFI GACNNNGTC 1 cut(s) 524
PfoI TCCNGGA 2 cut(s) 516, 585
PkrI GCNGC 6 cut(s) 60, 63, 217, 456, 535, 809
PleI GAGTC 2 cut(s) 398, 666
PpsI GAGTC 2 cut(s) 398, 666
Psp6I CCWGG 3 cut(s) 202, 516, 689
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 3 cut(s) 202, 516, 689
PspN4I GGNNCC 1 cut(s) 479
PspPI GGNCC 2 cut(s) 206, 526
PstI CTGCAG 1 cut(s) 890
PsyI GACNNNGTC 1 cut(s) 524
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 726, 831, 1018
SatI GCNGC 6 cut(s) 59, 62, 216, 455, 534, 808
Sau3AI GATC 6 cut(s) 148, 439, 496, 649, 708, 733
Sau96I GGNCC 2 cut(s) 206, 526
SchI GAGTC 2 cut(s) 398, 667
ScrFI CCNGG 4 cut(s) 204, 518, 587, 691
SfaNI GCATC 3 cut(s) 169, 621, 937
SfcI CTRYAG 2 cut(s) 396, 886
Sfr274I CTCGAG 1 cut(s) 443
SgrAI CRCCGGYG 1 cut(s) 112
SinI GGWCC 2 cut(s) 206, 526
SlaI CTCGAG 1 cut(s) 443
SmlI CTYRAG 3 cut(s) 287, 443, 719
SmoI CTYRAG 3 cut(s) 287, 443, 719
SphI GCATGC 1 cut(s) 910
Sse9I AATT 3 cut(s) 82, 741, 826
SseBI AGGCCT 1 cut(s) 884
SsiI CCGC 3 cut(s) 116, 164, 454
StuI AGGCCT 1 cut(s) 884
StyD4I CCNGG 4 cut(s) 202, 516, 585, 689
TaaI ACNGT 2 cut(s) 101, 782
TaiI ACGT 1 cut(s) 878
TaqI TCGA 4 cut(s) 96, 129, 444, 499
TasI AATT 3 cut(s) 82, 741, 826
TauI GCSGC 1 cut(s) 457
TfiI GAWTC 1 cut(s) 269
Tru1I TTAA 3 cut(s) 726, 831, 1018
Tru9I TTAA 3 cut(s) 726, 831, 1018
TscAI CASTG 2 cut(s) 235, 787
TseFI GTSAC 2 cut(s) 230, 776
TseI GCWGC 5 cut(s) 58, 61, 215, 533, 807
Tsp45I GTSAC 2 cut(s) 230, 776
TspDTI ATGAA 3 cut(s) 173, 714, 912
TspRI CASTG 2 cut(s) 235, 787
Tth111I GACNNNGTC 1 cut(s) 524
VpaK11BI GGWCC 2 cut(s) 206, 526
XagI CCTNNNNNAGG 1 cut(s) 521
XapI RAATTY 1 cut(s) 82
XceI RCATGY 1 cut(s) 910
XhoI CTCGAG 1 cut(s) 443
XmiI GTMKAC 2 cut(s) 395, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.