MD15G1080800.v1.1

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010150
Physical Location & Seq
Reverse (-)
5527121 .. 5530223
3103 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1080800.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGCTCTTCCCCAGCACCATTTCCAACAGCACTACCCACCCCAACAACAACAACAACAACAACAGCAACAATCCAAGTCTTTCAGAAATTTGTACACAATCGACGGTCAGGTGTCGCCGGCGGTGGCCTATTATGACCCCGGAAATTTACAAGATCACCCCCAGCAGCATCCTCCTTACGTTCCGCCCTTTCATGTAGAAGGGTTTGCGCCTGGTCCCGTGGCTGCAACGGATGGCAGCGATAATGGCGCTGATTTGCAGTGGCATTACGGTTTTGAATCCAAAAGGAAAAAGCTAAAGGAACAGGATTTCCTGGAGAACAACTCGCAGATATCGTCCATTGATTTCCTGCAACCACGATCGGTCTCCACAGGCTTGGGTTTGTCCCTTGACAATACTCGAATGGTTTCTACAGGGGACTCATCATTGCTGTCAGTTATCGGCGATGATGTTGATCATGAGCTACAGCGACAGGATGCTGAGATTGATAGATTCCTCAAGGCCCAGGGTGACCGATTGCGGCAAACTATCCTGGACAAGGTCCAAGCCACCCAGCTTCAGACTCTCTCAGCTGTGGAAGACAAAGTTCTTCGGAAGCTGCGAGAGAAAGAAGCAGAAGTGGAGAGCATTAGCAAGAAGAATATGGAGCTTGAAGAGCAAATGGAACAGTTGGCTGTTGAAGCAGGTGCTTGGCAACAGATAGCCAGGCGCAACGAAAACATGATCTCATCTCTAAGGTTTAATCTTCAGCACGTATATGCTCAAAGTAGAGATAGTAAAGAAGGATGCGGTGACAGTGAGGTAGATGATACAGCTTCTTGCTGCAATGGGCGTTCAATCAATTTGGATATGTTTGGCAAGGAGAATAATGACGGGAAAGAGATGATGACTTGCAAGGCCTGCCGGGTCAATGAAGTGTGCATGCTTCTATTGCCTTGTAAGCATCTTTGCCTGTGTAAAGATTGTGAAAGTAAGCTCAGCGTCTGCCCTCTCTGTCAGTCGTCTAAGTTCATCGGCATGGAGGTATATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

345

Amino Acids

38.86

Weight (kDa)

5.27

Isoelectric Point (pI)

57.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 296 - 334 1.5e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 674
Acc36I ACCTGC 1 cut(s) 674
AciI CCGC 4 cut(s) 122, 185, 520, 789
AcsI RAATTY 2 cut(s) 88, 145
AcuI CTGAAG 2 cut(s) 542, 731
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 1 cut(s) 538
AgsI TTSAA 4 cut(s) 278, 653, 680, 837
AjnI CCWGG 5 cut(s) 211, 312, 504, 531, 704
AloI GAACNNNNNNTCC 2 cut(s) 294, 326
AluBI AGCT 8 cut(s) 295, 463, 556, 572, 598, 649, 815, 976
AluI AGCT 8 cut(s) 295, 463, 556, 572, 598, 649, 815, 976
Alw26I GTCTC 1 cut(s) 370
AlwNI CAGNNNCTG 1 cut(s) 984
AoxI GGCC 3 cut(s) 126, 501, 897
ApeKI GCWGC 5 cut(s) 166, 224, 237, 598, 822
ApoI RAATTY 2 cut(s) 88, 145
Asp700I GAANNNNTTC 1 cut(s) 406
AspLEI GCGC 3 cut(s) 211, 251, 711
AspS9I GGNCC 3 cut(s) 215, 502, 541
AsuC2I CCSGG 2 cut(s) 141, 905
AsuHPI GGTGA 3 cut(s) 149, 521, 803
AvaII GGWCC 2 cut(s) 215, 541
BbsI GAAGAC 1 cut(s) 585
BbvI GCAGC 5 cut(s) 178, 211, 249, 585, 809
BccI CCATC 1 cut(s) 227
BciT130I CCWGG 5 cut(s) 213, 314, 506, 533, 706
BclI TGATCA 1 cut(s) 454
BcnI CCSGG 2 cut(s) 141, 905
BcoDI GTCTC 1 cut(s) 370
BfmI CTRYAG 2 cut(s) 411, 464
BfoI RGCGCY 1 cut(s) 252
BfuAI ACCTGC 1 cut(s) 674
BisI GCNGC 6 cut(s) 167, 225, 238, 521, 599, 823
BlpI GCTNAGC 1 cut(s) 977
BlsI GCNGC 6 cut(s) 168, 226, 239, 522, 600, 824
Bme1390I CCNGG 7 cut(s) 141, 213, 314, 506, 533, 706, 905
Bme18I GGWCC 2 cut(s) 215, 541
BmgT120I GGNCC 3 cut(s) 215, 502, 541
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 7 cut(s) 141, 213, 314, 506, 533, 706, 905
BmsI GCATC 4 cut(s) 178, 466, 776, 952
BpiI GAAGAC 1 cut(s) 585
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 1 cut(s) 335
Bpu1102I GCTNAGC 1 cut(s) 977
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 2 cut(s) 141, 905
BsaAI YACGTR 1 cut(s) 754
BsaBI GATNNNNATC 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 370
BsaJI CCNNGG 4 cut(s) 139, 219, 504, 505
Bsc4I CCNNNNNNNGG 1 cut(s) 538
Bse118I RCCGGY 1 cut(s) 118
Bse3DI GCAATG 2 cut(s) 425, 832
Bse8I GATNNNNATC 1 cut(s) 453
BseBI CCWGG 5 cut(s) 213, 314, 506, 533, 706
BseDI CCNNGG 4 cut(s) 139, 219, 504, 505
BseGI GGATG 4 cut(s) 169, 238, 481, 791
BseJI GATNNNNATC 1 cut(s) 453
BseLI CCNNNNNNNGG 1 cut(s) 538
BseMI GCAATG 2 cut(s) 425, 832
BseMII CTCAG 3 cut(s) 471, 582, 991
BseXI GCAGC 5 cut(s) 178, 211, 249, 585, 809
BseYI CCCAGC 3 cut(s) 12, 162, 552
Bsh1285I CGRYCG 1 cut(s) 362
BshFI GGCC 3 cut(s) 128, 503, 899
BsiEI CGRYCG 1 cut(s) 362
BsiSI CCGG 3 cut(s) 119, 141, 904
BslFI GGGAC 3 cut(s) 201, 370, 431
BslI CCNNNNNNNGG 1 cut(s) 538
BsmAI GTCTC 1 cut(s) 370
BsmFI GGGAC 3 cut(s) 201, 370, 431
BsnI GGCC 3 cut(s) 128, 503, 899
Bso31I GGTCTC 1 cut(s) 370
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 4 cut(s) 154, 359, 454, 723
Bsp1720I GCTNAGC 1 cut(s) 977
BspACI CCGC 4 cut(s) 122, 185, 520, 789
BspANI GGCC 3 cut(s) 128, 503, 899
BspCNI CTCAG 3 cut(s) 472, 581, 990
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 1 cut(s) 217
BspMI ACCTGC 1 cut(s) 674
BspQI GCTCTTC 2 cut(s) 12, 648
BspTNI GGTCTC 1 cut(s) 370
BsrDI GCAATG 2 cut(s) 425, 832
BsrFI RCCGGY 1 cut(s) 118
BsrGI TGTACA 1 cut(s) 93
BssAI RCCGGY 1 cut(s) 118
BssECI CCNNGG 4 cut(s) 139, 219, 504, 505
BssMI GATC 4 cut(s) 154, 359, 454, 723
Bst2UI CCWGG 5 cut(s) 213, 314, 506, 533, 706
Bst4CI ACNGT 4 cut(s) 107, 272, 669, 797
Bst6I CTCTTC 2 cut(s) 12, 648
BstAPI GCANNNNNTGC 1 cut(s) 900
BstAUI TGTACA 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 754
BstC8I GCNNGC 3 cut(s) 120, 901, 923
BstDEI CTNAG 5 cut(s) 480, 568, 734, 977, 1005
BstDSI CCRYGG 1 cut(s) 219
BstEII GGTNACC 1 cut(s) 509
BstENI CCTNNNNNAGG 1 cut(s) 536
BstF5I GGATG 4 cut(s) 169, 238, 481, 791
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 3 cut(s) 211, 251, 711
BstKTI GATC 4 cut(s) 157, 362, 457, 726
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 4 cut(s) 154, 359, 454, 723
BstMCI CGRYCG 1 cut(s) 362
BstMWI GCNNNNNNNGC 6 cut(s) 246, 655, 680, 900, 931, 940
BstNI CCWGG 5 cut(s) 213, 314, 506, 533, 706
BstNSI RCATGY 1 cut(s) 925
BstPI GGTNACC 1 cut(s) 509
BstSCI CCNGG 7 cut(s) 139, 211, 312, 504, 531, 704, 903
BstSFI CTRYAG 2 cut(s) 411, 464
BstV1I GCAGC 5 cut(s) 178, 211, 249, 585, 809
BstV2I GAAGAC 1 cut(s) 585
BstXI CCANNNNNNTGG 1 cut(s) 376
BsuRI GGCC 3 cut(s) 128, 503, 899
BtgI CCRYGG 1 cut(s) 219
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 4 cut(s) 169, 238, 481, 791
BtsI GCAGTG 1 cut(s) 266
BtsIMutI CAGTG 2 cut(s) 266, 802
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 3 cut(s) 120, 901, 923
CaiI CAGNNNCTG 1 cut(s) 984
CciI TCATGA 1 cut(s) 457
CfoI GCGC 3 cut(s) 211, 251, 711
Cfr10I RCCGGY 1 cut(s) 118
Cfr13I GGNCC 3 cut(s) 215, 502, 541
CseI GACGC 1 cut(s) 970
Csp6I GTAC 1 cut(s) 94
CviAII CATG 5 cut(s) 194, 458, 721, 922, 1018
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 5 cut(s) 480, 568, 734, 977, 1005
DpnI GATC 4 cut(s) 156, 361, 456, 725
DpnII GATC 4 cut(s) 154, 359, 454, 723
Eam1104I CTCTTC 2 cut(s) 12, 648
EarI CTCTTC 2 cut(s) 12, 648
EciI GGCGGA 1 cut(s) 174
Eco147I AGGCCT 1 cut(s) 899
Eco31I GGTCTC 1 cut(s) 370
Eco32I GATATC 1 cut(s) 333
Eco47I GGWCC 2 cut(s) 215, 541
Eco57I CTGAAG 2 cut(s) 542, 731
Eco91I GGTNACC 1 cut(s) 509
EcoNI CCTNNNNNAGG 1 cut(s) 536
EcoO65I GGTNACC 1 cut(s) 509
EcoRII CCWGG 5 cut(s) 211, 312, 504, 531, 704
EcoRV GATATC 1 cut(s) 333
FaeI CATG 5 cut(s) 197, 461, 724, 925, 1021
FaqI GGGAC 3 cut(s) 201, 370, 431
FatI CATG 5 cut(s) 193, 457, 720, 921, 1017
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 6 cut(s) 167, 225, 238, 521, 599, 823
FokI GGATG 4 cut(s) 156, 245, 488, 798
Fsp4HI GCNGC 6 cut(s) 167, 225, 238, 521, 599, 823
GlaI GCGC 3 cut(s) 210, 250, 710
GluI GCNGC 6 cut(s) 167, 225, 238, 521, 599, 823
GsaI CCCAGC 3 cut(s) 16, 166, 556
GsuI CTGGAG 1 cut(s) 335
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 3 cut(s) 128, 503, 899
HapII CCGG 3 cut(s) 119, 141, 904
HgaI GACGC 1 cut(s) 970
HhaI GCGC 3 cut(s) 211, 251, 711
Hin1II CATG 5 cut(s) 197, 461, 724, 925, 1021
Hin6I GCGC 3 cut(s) 209, 249, 709
HinP1I GCGC 3 cut(s) 209, 249, 709
HinfI GANTC 4 cut(s) 278, 419, 492, 562
HpaII CCGG 3 cut(s) 119, 141, 904
HphI GGTGA 3 cut(s) 149, 521, 803
Hpy166II GTNNAC 1 cut(s) 96
Hpy188I TCNGA 3 cut(s) 86, 561, 594
Hpy188III TCNNGA 1 cut(s) 458
Hpy8I GTNNAC 1 cut(s) 96
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 2 cut(s) 194, 776
HpyCH4III ACNGT 4 cut(s) 107, 272, 669, 797
HpyCH4IV ACGT 2 cut(s) 180, 753
HpyCH4V TGCA 6 cut(s) 227, 259, 352, 825, 894, 921
HpyF10VI GCNNNNNNNGC 6 cut(s) 246, 655, 680, 900, 931, 940
HpyF3I CTNAG 5 cut(s) 480, 568, 734, 977, 1005
HpySE526I ACGT 2 cut(s) 180, 753
Hsp92II CATG 5 cut(s) 197, 461, 724, 925, 1021
HspAI GCGC 3 cut(s) 209, 249, 709
KroI GCCGGC 1 cut(s) 118
KroNI GCCGGC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 4 cut(s) 154, 359, 454, 723
LguI GCTCTTC 2 cut(s) 12, 648
LmnI GCTCC 1 cut(s) 646
Lsp1109I GCAGC 5 cut(s) 178, 211, 249, 585, 809
LweI GCATC 4 cut(s) 178, 466, 776, 952
MaeII ACGT 2 cut(s) 180, 753
MaeIII GTNAC 2 cut(s) 509, 791
MalI GATC 4 cut(s) 156, 361, 456, 725
MboI GATC 4 cut(s) 154, 359, 454, 723
MboII GAAGA 5 cut(s) 581, 590, 649, 665, 737
MluCI AATT 3 cut(s) 88, 145, 841
MlyI GAGTC 2 cut(s) 413, 556
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 5 cut(s) 183, 506, 793, 999, 1015
MreI CGCCGGCG 1 cut(s) 118
MroNI GCCGGC 1 cut(s) 118
MroXI GAANNNNTTC 1 cut(s) 406
MseI TTAA 1 cut(s) 741
MslI CAYNNNNRTG 2 cut(s) 756, 1016
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 3 cut(s) 119, 141, 904
MspR9I CCNGG 7 cut(s) 141, 213, 314, 506, 533, 706, 905
MvaI CCWGG 5 cut(s) 213, 314, 506, 533, 706
MwoI GCNNNNNNNGC 6 cut(s) 246, 655, 680, 900, 931, 940
NaeI GCCGGC 1 cut(s) 120
NciI CCSGG 2 cut(s) 141, 905
NdeII GATC 4 cut(s) 154, 359, 454, 723
NgoMIV GCCGGC 1 cut(s) 118
NlaIII CATG 5 cut(s) 197, 461, 724, 925, 1021
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 2 cut(s) 509, 791
NspI RCATGY 1 cut(s) 925
PaeI GCATGC 1 cut(s) 925
PagI TCATGA 1 cut(s) 457
PaqCI CACCTGC 1 cut(s) 674
PasI CCCWGGG 1 cut(s) 505
PceI AGGCCT 1 cut(s) 899
PciSI GCTCTTC 2 cut(s) 12, 648
PcsI WCGNNNNNNNCGW 2 cut(s) 332, 598
PdiI GCCGGC 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 406
PfeI GAWTC 2 cut(s) 278, 492
PflFI GACNNNGTC 1 cut(s) 539
PfoI TCCNGGA 2 cut(s) 312, 531
PkrI GCNGC 6 cut(s) 168, 226, 239, 522, 600, 824
Ple19I CGATCG 1 cut(s) 362
PleI GAGTC 2 cut(s) 413, 556
PpsI GAGTC 2 cut(s) 413, 556
Ppu21I YACGTR 1 cut(s) 754
Psp6I CCWGG 5 cut(s) 211, 312, 504, 531, 704
PspEI GGTNACC 1 cut(s) 509
PspFI CCCAGC 3 cut(s) 12, 162, 552
PspGI CCWGG 5 cut(s) 211, 312, 504, 531, 704
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 3 cut(s) 215, 502, 541
PstNI CAGNNNCTG 1 cut(s) 984
PsyI GACNNNGTC 1 cut(s) 539
PvuI CGATCG 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 2 cut(s) 756, 1016
SapI GCTCTTC 2 cut(s) 12, 648
SaqAI TTAA 1 cut(s) 741
SatI GCNGC 6 cut(s) 167, 225, 238, 521, 599, 823
Sau3AI GATC 4 cut(s) 154, 359, 454, 723
Sau96I GGNCC 3 cut(s) 215, 502, 541
SchI GAGTC 2 cut(s) 413, 556
ScrFI CCNGG 7 cut(s) 141, 213, 314, 506, 533, 706, 905
SfaNI GCATC 4 cut(s) 178, 466, 776, 952
SfcI CTRYAG 2 cut(s) 411, 464
SgrAI CRCCGGYG 1 cut(s) 118
SinI GGWCC 2 cut(s) 215, 541
SmiMI CAYNNNNRTG 2 cut(s) 756, 1016
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
SphI GCATGC 1 cut(s) 925
Sse9I AATT 3 cut(s) 88, 145, 841
SseBI AGGCCT 1 cut(s) 899
SsiI CCGC 4 cut(s) 122, 185, 520, 789
StuI AGGCCT 1 cut(s) 899
StyD4I CCNGG 7 cut(s) 139, 211, 312, 504, 531, 704, 903
TaaI ACNGT 4 cut(s) 107, 272, 669, 797
TaiI ACGT 2 cut(s) 183, 756
TaqI TCGA 2 cut(s) 102, 400
TaqII GACCGA 2 cut(s) 352, 528
TasI AATT 3 cut(s) 88, 145, 841
TatI WGTACW 1 cut(s) 93
TauI GCSGC 1 cut(s) 523
TfiI GAWTC 2 cut(s) 278, 492
Tru1I TTAA 1 cut(s) 741
Tru9I TTAA 1 cut(s) 741
TscAI CASTG 2 cut(s) 266, 802
TseFI GTSAC 2 cut(s) 509, 791
TseI GCWGC 5 cut(s) 166, 224, 237, 598, 822
Tsp45I GTSAC 2 cut(s) 509, 791
TspDTI ATGAA 3 cut(s) 182, 927, 1000
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 2 cut(s) 266, 802
Tth111I GACNNNGTC 1 cut(s) 539
VpaK11BI GGWCC 2 cut(s) 215, 541
XagI CCTNNNNNAGG 1 cut(s) 536
XapI RAATTY 2 cut(s) 88, 145
XceI RCATGY 1 cut(s) 925
XmnI GAANNNNTTC 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.