RLG00000010662

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010150
Physical Location & Seq
Forward (+)
3409027 .. 3413781
4755 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010662

Sequence Viewer

Length: 1038 bp
ATGGCTCTTCCCCAACACCACTTTCAACAACACTATCCACCCCAACAACAACAGCAGCAGCAGCAGCAATCCAAGTCATTCAGAAATTTATACACAATCGACGGTCAGATGTCGCCGGCGGTGGCCTATTTCGACCCCAACGCCTTACAAGATCACTCCCAGCATCCGCCTTATGTTCCACCGTTTCATGTAGTAGGGTTTGCGCCTGGTCCGGTGCCTGGAACAGACGGCAGTGACAATGGTGCTGATTTTCATTGGAACTATGGCTTAGAATCCAAGAGGAAAAGGCTCAAGGAGCAGGATTTTTTGGAGAACAACTCGCAAATGTCTTCGGTTCTGCAACAGCAATCAGTCTCAACGGGCTTGGGTCTATCCCTAGACAATAATCGCATGGCCTCTACAGGGGACTCGGCTTTGCTGTCTCTCATTGGCGAGGATGTTGATCTCGAGATACAGCGGCAAGATGTTGAGATTGATAGGTTCCTCAAAGCCCAGAGTGATCGATTGCGACAAAATATCCTGGACAAGGTCCAAGCTGCCCAACTTCAAACTCTCTCAGTTGTAGAAGAAAAAGTACTCCAGAAGCTCCGGGAAAAAGAAGCAGAAGTCGAGAGCATCAACAAGAAGAATATGGAGCTTGAAGAAAGAATGGATCAGTTGAGTCTTGAAGCAAGGATTTGGCAACATCAAGCCAGGAACAATGAACACATGATCACAGCTCTCAAGGTTAATCTTGATCAAGTAATTGCTCAAAGTAGAGACAGCAAGGAAGGATGTGGTGACAGTGAAATAGATGATACAGCTTCCTGCTGCAATGGCCATTCCATCAATTTTAATATGTTTGGCAAGGAGAGCAGTGATGTGAAAGAGTTCGTACAGCTTTTGCTTGGTTCCAATGGCAACCATTCCAGGACAGTTGATTTGGGAGATTGTCAAGAAGAACAACTCCTTGGTCAAGCAGTTCGGTTGGAGCCAAGCCGGCGTCCGTCGGTCCGTTTCAACAAGGAGCCTAACAACCTCTACAACCTCCACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

346

Amino Acids

39.08

Weight (kDa)

5.25

Isoelectric Point (pI)

58.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 214
AciI CCGC 3 cut(s) 119, 167, 457
AclWI GGATC 1 cut(s) 660
AcoI YGGCCR 1 cut(s) 817
AcsI RAATTY 1 cut(s) 85
AcyI GRCGYC 1 cut(s) 982
AfaI GTAC 2 cut(s) 576, 876
AfiI CCNNNNNNNGG 3 cut(s) 218, 402, 526
AgsI TTSAA 5 cut(s) 26, 548, 641, 668, 1000
AjnI CCWGG 5 cut(s) 205, 217, 519, 692, 908
AjuI GAANNNNNNNTTGG 3 cut(s) 38, 933, 965
AluBI AGCT 6 cut(s) 536, 586, 637, 719, 803, 880
AluI AGCT 6 cut(s) 536, 586, 637, 719, 803, 880
Alw26I GTCTC 3 cut(s) 358, 426, 753
AlwI GGATC 1 cut(s) 660
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 3 cut(s) 123, 393, 817
ApeKI GCWGC 6 cut(s) 55, 58, 61, 64, 536, 810
ApoI RAATTY 1 cut(s) 85
ArsI GACNNNNNNTTYG 2 cut(s) 904, 936
Asp700I GAANNNNTTC 1 cut(s) 869
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 3 cut(s) 209, 529, 991
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 1 cut(s) 791
AvaI CYCGRG 1 cut(s) 446
AvaII GGWCC 3 cut(s) 209, 529, 991
BalI TGGCCA 1 cut(s) 819
BanI GGYRCC 1 cut(s) 214
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
BbsI GAAGAC 1 cut(s) 321
BbvI GCAGC 6 cut(s) 67, 70, 73, 76, 523, 797
BccI CCATC 1 cut(s) 833
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 5 cut(s) 207, 219, 521, 694, 910
BclI TGATCA 2 cut(s) 711, 736
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 3 cut(s) 358, 426, 753
BfaI CTAG 1 cut(s) 377
BfmI CTRYAG 1 cut(s) 399
BglI GCCNNNNNGGC 1 cut(s) 979
BisI GCNGC 7 cut(s) 56, 59, 62, 65, 458, 537, 811
BlsI GCNGC 7 cut(s) 57, 60, 63, 66, 459, 538, 812
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 6 cut(s) 207, 219, 521, 590, 694, 910
Bme18I GGWCC 3 cut(s) 209, 529, 991
BmeT110I CYCGRG 1 cut(s) 446
BmgT120I GGNCC 3 cut(s) 209, 529, 991
BmiI GGNNCC 5 cut(s) 216, 482, 892, 972, 1008
BmrFI CCNGG 6 cut(s) 207, 219, 521, 590, 694, 910
BmsI GCATC 2 cut(s) 172, 624
BpiI GAAGAC 1 cut(s) 321
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 1 cut(s) 563
BpuEI CTTGAG 2 cut(s) 275, 707
BpuMI CCSGG 1 cut(s) 590
Bsa29I ATCGAT 1 cut(s) 502
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 1 cut(s) 982
BsaJI CCNNGG 1 cut(s) 949
BsaWI WCCGGW 1 cut(s) 211
BsaXI ACNNNNNCTCC 1 cut(s) 1016
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 402, 526
Bse118I RCCGGY 2 cut(s) 115, 978
Bse3DI GCAATG 1 cut(s) 820
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 5 cut(s) 207, 219, 521, 694, 910
BseCI ATCGAT 1 cut(s) 502
BseDI CCNNGG 1 cut(s) 949
BseGI GGATG 3 cut(s) 163, 442, 779
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 3 cut(s) 218, 402, 526
BseMI GCAATG 1 cut(s) 820
BseMII CTCAG 1 cut(s) 570
BseXI GCAGC 6 cut(s) 67, 70, 73, 76, 523, 797
BseYI CCCAGC 1 cut(s) 159
BshFI GGCC 3 cut(s) 125, 395, 819
BshNI GGYRCC 1 cut(s) 214
BshVI ATCGAT 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 446
BsiSI CCGG 4 cut(s) 116, 212, 589, 979
BslFI GGGAC 1 cut(s) 419
BslI CCNNNNNNNGG 3 cut(s) 218, 402, 526
BsmAI GTCTC 3 cut(s) 358, 426, 753
BsmFI GGGAC 1 cut(s) 419
BsnI GGCC 3 cut(s) 125, 395, 819
BsoBI CYCGRG 1 cut(s) 446
Bsp143I GATC 6 cut(s) 151, 442, 499, 652, 711, 736
BspACI CCGC 3 cut(s) 119, 167, 457
BspANI GGCC 3 cut(s) 125, 395, 819
BspCNI CTCAG 1 cut(s) 569
BspDI ATCGAT 1 cut(s) 502
BspLI GGNNCC 5 cut(s) 216, 482, 892, 972, 1008
BspPI GGATC 1 cut(s) 660
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 820
BsrFI RCCGGY 2 cut(s) 115, 978
BssAI RCCGGY 2 cut(s) 115, 978
BssECI CCNNGG 1 cut(s) 949
BssMI GATC 6 cut(s) 151, 442, 499, 652, 711, 736
BssNI GRCGYC 1 cut(s) 982
BssT1I CCWWGG 1 cut(s) 949
Bst2UI CCWGG 5 cut(s) 207, 219, 521, 694, 910
Bst4CI ACNGT 4 cut(s) 104, 183, 785, 916
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 982
BstC8I GCNNGC 2 cut(s) 117, 980
BstDEI CTNAG 2 cut(s) 268, 556
BstENI CCTNNNNNAGG 1 cut(s) 524
BstF5I GGATG 3 cut(s) 163, 442, 779
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 6 cut(s) 154, 445, 502, 655, 714, 739
BstMAI GTCTC 3 cut(s) 358, 426, 753
BstMBI GATC 6 cut(s) 151, 442, 499, 652, 711, 736
BstMWI GCNNNNNNNGC 6 cut(s) 61, 64, 295, 816, 852, 979
BstNI CCWGG 5 cut(s) 207, 219, 521, 694, 910
BstSCI CCNGG 6 cut(s) 205, 217, 519, 588, 692, 908
BstSFI CTRYAG 1 cut(s) 399
BstV1I GCAGC 6 cut(s) 67, 70, 73, 76, 523, 797
BstV2I GAAGAC 1 cut(s) 321
Bsu15I ATCGAT 1 cut(s) 502
BsuRI GGCC 3 cut(s) 125, 395, 819
BsuTUI ATCGAT 1 cut(s) 502
BtsCI GGATG 3 cut(s) 163, 442, 779
BtsI GCAGTG 2 cut(s) 238, 862
BtsIMutI CAGTG 3 cut(s) 238, 790, 862
Cac8I GCNNGC 2 cut(s) 117, 980
CfoI GCGC 1 cut(s) 205
Cfr10I RCCGGY 2 cut(s) 115, 978
Cfr13I GGNCC 3 cut(s) 209, 529, 991
ClaI ATCGAT 1 cut(s) 502
CpoI CGGWCCG 1 cut(s) 991
CseI GACGC 1 cut(s) 971
Csp6I GTAC 2 cut(s) 575, 875
CspI CGGWCCG 1 cut(s) 991
CviAII CATG 3 cut(s) 188, 391, 709
CviQI GTAC 2 cut(s) 575, 875
DdeI CTNAG 2 cut(s) 268, 556
DpnI GATC 6 cut(s) 153, 444, 501, 654, 713, 738
DpnII GATC 6 cut(s) 151, 442, 499, 652, 711, 736
EaeI YGGCCR 1 cut(s) 817
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 949
Eco47I GGWCC 3 cut(s) 209, 529, 991
Eco88I CYCGRG 1 cut(s) 446
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoRII CCWGG 5 cut(s) 205, 217, 519, 692, 908
EcoT14I CCWWGG 1 cut(s) 949
ErhI CCWWGG 1 cut(s) 949
FaeI CATG 3 cut(s) 191, 394, 712
FaiI YATR 8 cut(s) 91, 174, 189, 264, 392, 632, 710, 839
FaqI GGGAC 1 cut(s) 419
FatI CATG 3 cut(s) 187, 390, 708
FbaI TGATCA 2 cut(s) 711, 736
Fnu4HI GCNGC 7 cut(s) 56, 59, 62, 65, 458, 537, 811
FokI GGATG 3 cut(s) 150, 449, 786
Fsp4HI GCNGC 7 cut(s) 56, 59, 62, 65, 458, 537, 811
FspBI CTAG 1 cut(s) 377
GlaI GCGC 1 cut(s) 204
GluI GCNGC 7 cut(s) 56, 59, 62, 65, 458, 537, 811
GsaI CCCAGC 1 cut(s) 163
GsuI CTGGAG 1 cut(s) 563
HaeIII GGCC 3 cut(s) 125, 395, 819
HapII CCGG 4 cut(s) 116, 212, 589, 979
HgaI GACGC 1 cut(s) 971
HhaI GCGC 1 cut(s) 205
Hin1I GRCGYC 1 cut(s) 982
Hin1II CATG 3 cut(s) 191, 394, 712
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HinfI GANTC 3 cut(s) 272, 407, 661
HpaII CCGG 4 cut(s) 116, 212, 589, 979
HphI GGTGA 1 cut(s) 791
Hpy188I TCNGA 2 cut(s) 83, 108
Hpy188III TCNNGA 7 cut(s) 446, 448, 580, 610, 665, 734, 935
Hpy99I CGWCG 2 cut(s) 104, 991
HpyAV CCTTC 1 cut(s) 764
HpyCH4III ACNGT 4 cut(s) 104, 183, 785, 916
HpyCH4V TGCA 2 cut(s) 340, 813
HpyF10VI GCNNNNNNNGC 6 cut(s) 61, 64, 295, 816, 852, 979
HpyF3I CTNAG 2 cut(s) 268, 556
Hsp92I GRCGYC 1 cut(s) 982
Hsp92II CATG 3 cut(s) 191, 394, 712
HspAI GCGC 1 cut(s) 203
KroI GCCGGC 2 cut(s) 115, 978
KroNI GCCGGC 2 cut(s) 117, 980
Ksp22I TGATCA 2 cut(s) 711, 736
Kzo9I GATC 6 cut(s) 151, 442, 499, 652, 711, 736
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 5 cut(s) 295, 591, 634, 970, 1006
Lsp1109I GCAGC 6 cut(s) 67, 70, 73, 76, 523, 797
LweI GCATC 2 cut(s) 172, 624
MaeI CTAG 1 cut(s) 377
MaeIII GTNAC 2 cut(s) 233, 779
MalI GATC 6 cut(s) 153, 444, 501, 654, 713, 738
MboI GATC 6 cut(s) 151, 442, 499, 652, 711, 736
MboII GAAGA 5 cut(s) 321, 578, 637, 653, 950
MlsI TGGCCA 1 cut(s) 819
MluCI AATT 3 cut(s) 85, 744, 829
MluNI TGGCCA 1 cut(s) 819
MlyI GAGTC 2 cut(s) 401, 670
MmeI TCCRAC 1 cut(s) 948
MnlI CCTC 6 cut(s) 273, 406, 427, 494, 1028, 1037
Mox20I TGGCCA 1 cut(s) 819
MreI CGCCGGCG 1 cut(s) 115
MroNI GCCGGC 2 cut(s) 115, 978
MroXI GAANNNNTTC 1 cut(s) 869
MscI TGGCCA 1 cut(s) 819
MseI TTAA 2 cut(s) 729, 834
Msp20I TGGCCA 1 cut(s) 819
MspA1I CMGCKG 1 cut(s) 457
MspI CCGG 4 cut(s) 116, 212, 589, 979
MspR9I CCNGG 6 cut(s) 207, 219, 521, 590, 694, 910
MvaI CCWGG 5 cut(s) 207, 219, 521, 694, 910
MwoI GCNNNNNNNGC 6 cut(s) 61, 64, 295, 816, 852, 979
NaeI GCCGGC 2 cut(s) 117, 980
NciI CCSGG 1 cut(s) 590
NdeII GATC 6 cut(s) 151, 442, 499, 652, 711, 736
NgoMIV GCCGGC 2 cut(s) 115, 978
NlaIII CATG 3 cut(s) 191, 394, 712
NlaIV GGNNCC 5 cut(s) 216, 482, 892, 972, 1008
NmeAIII GCCGAG 1 cut(s) 389
NmuCI GTSAC 2 cut(s) 233, 779
PaeR7I CTCGAG 1 cut(s) 446
PciSI GCTCTTC 1 cut(s) 12
PdiI GCCGGC 2 cut(s) 117, 980
PdmI GAANNNNTTC 1 cut(s) 869
PfeI GAWTC 1 cut(s) 272
PflFI GACNNNGTC 1 cut(s) 527
PfoI TCCNGGA 3 cut(s) 519, 588, 908
PkrI GCNGC 7 cut(s) 57, 60, 63, 66, 459, 538, 812
PleI GAGTC 2 cut(s) 401, 669
PpsI GAGTC 2 cut(s) 401, 669
Psp6I CCWGG 5 cut(s) 205, 217, 519, 692, 908
PspFI CCCAGC 1 cut(s) 159
PspGI CCWGG 5 cut(s) 205, 217, 519, 692, 908
PspN4I GGNNCC 5 cut(s) 216, 482, 892, 972, 1008
PspPI GGNCC 3 cut(s) 209, 529, 991
PsyI GACNNNGTC 1 cut(s) 527
RsaI GTAC 2 cut(s) 576, 876
RsaNI GTAC 2 cut(s) 575, 875
Rsr2I CGGWCCG 1 cut(s) 991
RsrII CGGWCCG 1 cut(s) 991
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 729, 834
SatI GCNGC 7 cut(s) 56, 59, 62, 65, 458, 537, 811
Sau3AI GATC 6 cut(s) 151, 442, 499, 652, 711, 736
Sau96I GGNCC 3 cut(s) 209, 529, 991
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 2 cut(s) 401, 670
ScrFI CCNGG 6 cut(s) 207, 219, 521, 590, 694, 910
SfaNI GCATC 2 cut(s) 172, 624
SfcI CTRYAG 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 446
SgrAI CRCCGGYG 1 cut(s) 115
SinI GGWCC 3 cut(s) 209, 529, 991
SlaI CTCGAG 1 cut(s) 446
SmlI CTYRAG 3 cut(s) 290, 446, 722
SmoI CTYRAG 3 cut(s) 290, 446, 722
Sse9I AATT 3 cut(s) 85, 744, 829
SsiI CCGC 3 cut(s) 119, 167, 457
SspMI CTAG 1 cut(s) 377
StyD4I CCNGG 6 cut(s) 205, 217, 519, 588, 692, 908
StyI CCWWGG 1 cut(s) 949
TaaI ACNGT 4 cut(s) 104, 183, 785, 916
TaqI TCGA 5 cut(s) 99, 132, 447, 502, 609
TaqII GACCGA 1 cut(s) 979
TasI AATT 3 cut(s) 85, 744, 829
TatI WGTACW 1 cut(s) 574
TauI GCSGC 1 cut(s) 460
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 2 cut(s) 729, 834
Tru9I TTAA 2 cut(s) 729, 834
TscAI CASTG 3 cut(s) 238, 790, 862
TseFI GTSAC 2 cut(s) 233, 779
TseI GCWGC 6 cut(s) 55, 58, 61, 64, 536, 810
Tsp45I GTSAC 2 cut(s) 233, 779
TspDTI ATGAA 3 cut(s) 176, 242, 717
TspGWI ACGGA 2 cut(s) 975, 983
TspRI CASTG 3 cut(s) 238, 790, 862
Tth111I GACNNNGTC 1 cut(s) 527
VpaK11BI GGWCC 3 cut(s) 209, 529, 991
XagI CCTNNNNNAGG 1 cut(s) 524
XapI RAATTY 1 cut(s) 85
XhoI CTCGAG 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 869
XspI CTAG 1 cut(s) 377
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.