Rroxscaffold_7G00160390

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010150
Physical Location & Seq
Forward (+)
3366738 .. 3369819
3082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160390.1

Sequence Viewer

Length: 1023 bp
ATGGCTCTTCCCCAACACCACTTTCAGCAACACTATCCACCCCAACAACAACAACAACAGCAGCAGCAATCCAAGTCATTCAGAAATTTATACACAATCGACGGTCAGATGTCGCCGGCGGTGGCCTATTTCGACCCCAACGCCTTACAAGATCACCCCCAGCATCCGCCTTATGTTCCACCCTTTCATGTAGTAGGGTTTGCGCCTGGTCCGGTGGCTGCAACAGACGGCAGTGACAATGGTGCTGATTTTCAATGGAACTATGGCTTAGAATCCAAGAGGAAAAGGCTCAAGGAGCAGGATTTTTTGGAGAACAACTCGCAAATGTCTTCGGTTCTGCAACAGCAATCAGTCTCAACGGGCTTGGGTCTGTCCCTTGACAATAATCGCATGGCGTCTACAGGGGACTCGGCTTTGCTGTCTCTCATTGGCGAGGATGTTGATCTCGAGATACAGCGGCAAGATGTTGAGATTGATAGGTTCCTCAAAGCCCAGAGTGATCGATTGCGACAAAATATCCTGGACAAGGTCCAAGCTGCCCAACTTCAAACTCTCTCAGTTGTAGAAGAAAAAGTACTCCAGAAGCTCCGGGAAAAAGAAGCAGAAGTCGAGAGCATCAACAAGAAGAATATGGAGCTTGAAGAAAGAATGGATCAGTTGAGTCTTGAAGCAAGGATTTGGCAACATCAAGCCAGGAACAATGAACGCACGATCACAGCTCTCAAGGTTAATCTTGATCAAGTAATTGCTCAAAGTAGAGACAGCAAGGAAGGATGTGGTGACAGTGAAATAGATGATACAGCTTCCTGCTGCAATGGCCATTCCATCAATTTTAATATGTTTGGCAAGGAGAGCAATGATGTGAAAGAGGTAATGACGTGCAAGGCCTGCAGAGTGAATGAAGTTTGCATGCTTCTATTACCCTGTAAGCATCTTTGCCTGTGTAAAGATTGCGAATGTAAGCTTAGCATTTGTCCTCTGTGTCAGTCCTCTAAGTTTATCGGCATGGAAGTATACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

340

Amino Acids

38.53

Weight (kDa)

5.27

Isoelectric Point (pI)

57.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 292 - 330 9.3e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 398, 1014
AciI CCGC 3 cut(s) 119, 167, 457
AclWI GGATC 1 cut(s) 660
AcoI YGGCCR 1 cut(s) 817
AcsI RAATTY 1 cut(s) 85
AcyI GRCGYC 1 cut(s) 395
AfaI GTAC 1 cut(s) 576
AfiI CCNNNNNNNGG 1 cut(s) 526
AgsI TTSAA 4 cut(s) 254, 548, 641, 668
AjiI CACGTC 1 cut(s) 879
AjnI CCWGG 3 cut(s) 205, 519, 692
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AluBI AGCT 6 cut(s) 536, 586, 637, 719, 803, 964
AluI AGCT 6 cut(s) 536, 586, 637, 719, 803, 964
Alw26I GTCTC 3 cut(s) 358, 426, 753
AlwI GGATC 1 cut(s) 660
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 3 cut(s) 123, 817, 885
ApeKI GCWGC 5 cut(s) 61, 64, 218, 536, 810
ApoI RAATTY 1 cut(s) 85
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 2 cut(s) 209, 529
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 2 cut(s) 146, 791
AvaI CYCGRG 1 cut(s) 446
AvaII GGWCC 2 cut(s) 209, 529
BalI TGGCCA 1 cut(s) 819
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
BbsI GAAGAC 1 cut(s) 321
BbvI GCAGC 5 cut(s) 73, 76, 205, 523, 797
BccI CCATC 1 cut(s) 833
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 3 cut(s) 207, 521, 694
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 3 cut(s) 358, 426, 753
BfmI CTRYAG 2 cut(s) 399, 889
BisI GCNGC 6 cut(s) 62, 65, 219, 458, 537, 811
BlpI GCTNAGC 1 cut(s) 965
BlsI GCNGC 6 cut(s) 63, 66, 220, 459, 538, 812
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 4 cut(s) 207, 521, 590, 694
Bme18I GGWCC 2 cut(s) 209, 529
BmeT110I CYCGRG 1 cut(s) 446
BmgBI CACGTC 1 cut(s) 879
BmgT120I GGNCC 2 cut(s) 209, 529
BmiI GGNNCC 1 cut(s) 482
BmrFI CCNGG 4 cut(s) 207, 521, 590, 694
BmsI GCATC 3 cut(s) 172, 624, 940
BpiI GAAGAC 1 cut(s) 321
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 1 cut(s) 563
Bpu1102I GCTNAGC 1 cut(s) 965
BpuEI CTTGAG 2 cut(s) 275, 707
BpuMI CCSGG 1 cut(s) 590
Bsa29I ATCGAT 1 cut(s) 502
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 1 cut(s) 395
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse118I RCCGGY 1 cut(s) 115
Bse3DI GCAATG 2 cut(s) 820, 862
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 3 cut(s) 207, 521, 694
BseCI ATCGAT 1 cut(s) 502
BseGI GGATG 3 cut(s) 163, 442, 779
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMI GCAATG 2 cut(s) 820, 862
BseMII CTCAG 1 cut(s) 570
BseXI GCAGC 5 cut(s) 73, 76, 205, 523, 797
BseYI CCCAGC 1 cut(s) 159
BshFI GGCC 3 cut(s) 125, 819, 887
BshVI ATCGAT 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 446
BsiSI CCGG 3 cut(s) 116, 212, 589
BslFI GGGAC 2 cut(s) 358, 419
BslI CCNNNNNNNGG 1 cut(s) 526
BsmAI GTCTC 3 cut(s) 358, 426, 753
BsmFI GGGAC 2 cut(s) 358, 419
BsnI GGCC 3 cut(s) 125, 819, 887
BsoBI CYCGRG 1 cut(s) 446
Bsp143I GATC 6 cut(s) 151, 442, 499, 652, 711, 736
Bsp1720I GCTNAGC 1 cut(s) 965
BspACI CCGC 3 cut(s) 119, 167, 457
BspANI GGCC 3 cut(s) 125, 819, 887
BspCNI CTCAG 1 cut(s) 569
BspDI ATCGAT 1 cut(s) 502
BspLI GGNNCC 1 cut(s) 482
BspMAI CTGCAG 1 cut(s) 893
BspPI GGATC 1 cut(s) 660
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 820, 862
BsrFI RCCGGY 1 cut(s) 115
BssAI RCCGGY 1 cut(s) 115
BssMI GATC 6 cut(s) 151, 442, 499, 652, 711, 736
BssNAI GTATAC 1 cut(s) 1015
BssNI GRCGYC 1 cut(s) 395
Bst1107I GTATAC 1 cut(s) 1015
Bst2UI CCWGG 3 cut(s) 207, 521, 694
Bst4CI ACNGT 2 cut(s) 104, 785
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 395
BstAPI GCANNNNNTGC 1 cut(s) 888
BstC8I GCNNGC 3 cut(s) 117, 889, 911
BstDEI CTNAG 4 cut(s) 268, 556, 965, 993
BstENI CCTNNNNNAGG 1 cut(s) 524
BstF5I GGATG 3 cut(s) 163, 442, 779
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 6 cut(s) 154, 445, 502, 655, 714, 739
BstMAI GTCTC 3 cut(s) 358, 426, 753
BstMBI GATC 6 cut(s) 151, 442, 499, 652, 711, 736
BstMWI GCNNNNNNNGC 4 cut(s) 295, 816, 852, 888
BstNI CCWGG 3 cut(s) 207, 521, 694
BstNSI RCATGY 1 cut(s) 913
BstSCI CCNGG 4 cut(s) 205, 519, 588, 692
BstSFI CTRYAG 2 cut(s) 399, 889
BstV1I GCAGC 5 cut(s) 73, 76, 205, 523, 797
BstV2I GAAGAC 1 cut(s) 321
BstZ17I GTATAC 1 cut(s) 1015
Bsu15I ATCGAT 1 cut(s) 502
BsuRI GGCC 3 cut(s) 125, 819, 887
BsuTUI ATCGAT 1 cut(s) 502
BtrI CACGTC 1 cut(s) 879
BtsCI GGATG 3 cut(s) 163, 442, 779
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 2 cut(s) 238, 790
Cac8I GCNNGC 3 cut(s) 117, 889, 911
CfoI GCGC 1 cut(s) 205
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 2 cut(s) 209, 529
ClaI ATCGAT 1 cut(s) 502
CseI GACGC 1 cut(s) 384
Csp6I GTAC 1 cut(s) 575
CviAII CATG 4 cut(s) 188, 391, 910, 1006
CviQI GTAC 1 cut(s) 575
DdeI CTNAG 4 cut(s) 268, 556, 965, 993
DpnI GATC 6 cut(s) 153, 444, 501, 654, 713, 738
DpnII GATC 6 cut(s) 151, 442, 499, 652, 711, 736
EaeI YGGCCR 1 cut(s) 817
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 1 cut(s) 156
Eco147I AGGCCT 1 cut(s) 887
Eco47I GGWCC 2 cut(s) 209, 529
Eco88I CYCGRG 1 cut(s) 446
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoRII CCWGG 3 cut(s) 205, 519, 692
FaeI CATG 4 cut(s) 191, 394, 913, 1009
FaqI GGGAC 2 cut(s) 358, 419
FatI CATG 4 cut(s) 187, 390, 909, 1005
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 2 cut(s) 398, 1014
Fnu4HI GCNGC 6 cut(s) 62, 65, 219, 458, 537, 811
FokI GGATG 3 cut(s) 150, 449, 786
Fsp4HI GCNGC 6 cut(s) 62, 65, 219, 458, 537, 811
GlaI GCGC 1 cut(s) 204
GluI GCNGC 6 cut(s) 62, 65, 219, 458, 537, 811
GsaI CCCAGC 1 cut(s) 163
GsuI CTGGAG 1 cut(s) 563
HaeIII GGCC 3 cut(s) 125, 819, 887
HapII CCGG 3 cut(s) 116, 212, 589
HgaI GACGC 1 cut(s) 384
HhaI GCGC 1 cut(s) 205
Hin1I GRCGYC 1 cut(s) 395
Hin1II CATG 4 cut(s) 191, 394, 913, 1009
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HindIII AAGCTT 1 cut(s) 962
HinfI GANTC 3 cut(s) 272, 407, 661
HpaII CCGG 3 cut(s) 116, 212, 589
HphI GGTGA 2 cut(s) 146, 791
Hpy166II GTNNAC 2 cut(s) 399, 1015
Hpy188I TCNGA 2 cut(s) 83, 108
Hpy188III TCNNGA 6 cut(s) 446, 448, 580, 610, 665, 734
Hpy8I GTNNAC 2 cut(s) 399, 1015
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 1 cut(s) 764
HpyCH4III ACNGT 2 cut(s) 104, 785
HpyCH4IV ACGT 1 cut(s) 878
HpyCH4V TGCA 6 cut(s) 221, 340, 813, 882, 891, 909
HpyF10VI GCNNNNNNNGC 4 cut(s) 295, 816, 852, 888
HpyF3I CTNAG 4 cut(s) 268, 556, 965, 993
HpySE526I ACGT 1 cut(s) 878
Hsp92I GRCGYC 1 cut(s) 395
Hsp92II CATG 4 cut(s) 191, 394, 913, 1009
HspAI GCGC 1 cut(s) 203
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 6 cut(s) 151, 442, 499, 652, 711, 736
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 295, 591, 634
Lsp1109I GCAGC 5 cut(s) 73, 76, 205, 523, 797
LweI GCATC 3 cut(s) 172, 624, 940
MaeII ACGT 1 cut(s) 878
MaeIII GTNAC 2 cut(s) 233, 779
MalI GATC 6 cut(s) 153, 444, 501, 654, 713, 738
MboI GATC 6 cut(s) 151, 442, 499, 652, 711, 736
MboII GAAGA 4 cut(s) 321, 578, 637, 653
MlsI TGGCCA 1 cut(s) 819
MluCI AATT 3 cut(s) 85, 744, 829
MluNI TGGCCA 1 cut(s) 819
MlyI GAGTC 2 cut(s) 401, 670
MnlI CCTC 6 cut(s) 273, 427, 494, 862, 987, 1000
Mox20I TGGCCA 1 cut(s) 819
MreI CGCCGGCG 1 cut(s) 115
MroNI GCCGGC 1 cut(s) 115
MscI TGGCCA 1 cut(s) 819
MseI TTAA 3 cut(s) 729, 834, 1021
Msp20I TGGCCA 1 cut(s) 819
MspA1I CMGCKG 1 cut(s) 457
MspI CCGG 3 cut(s) 116, 212, 589
MspR9I CCNGG 4 cut(s) 207, 521, 590, 694
MvaI CCWGG 3 cut(s) 207, 521, 694
MwoI GCNNNNNNNGC 4 cut(s) 295, 816, 852, 888
NaeI GCCGGC 1 cut(s) 117
NciI CCSGG 1 cut(s) 590
NdeII GATC 6 cut(s) 151, 442, 499, 652, 711, 736
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 4 cut(s) 191, 394, 913, 1009
NlaIV GGNNCC 1 cut(s) 482
NmeAIII GCCGAG 1 cut(s) 389
NmuCI GTSAC 2 cut(s) 233, 779
NspI RCATGY 1 cut(s) 913
PaeI GCATGC 1 cut(s) 913
PaeR7I CTCGAG 1 cut(s) 446
PceI AGGCCT 1 cut(s) 887
PciSI GCTCTTC 1 cut(s) 12
PdiI GCCGGC 1 cut(s) 117
PfeI GAWTC 1 cut(s) 272
PflFI GACNNNGTC 1 cut(s) 527
PfoI TCCNGGA 2 cut(s) 519, 588
PkrI GCNGC 6 cut(s) 63, 66, 220, 459, 538, 812
PleI GAGTC 2 cut(s) 401, 669
PpsI GAGTC 2 cut(s) 401, 669
Psp6I CCWGG 3 cut(s) 205, 519, 692
PspFI CCCAGC 1 cut(s) 159
PspGI CCWGG 3 cut(s) 205, 519, 692
PspN4I GGNNCC 1 cut(s) 482
PspPI GGNCC 2 cut(s) 209, 529
PstI CTGCAG 1 cut(s) 893
PsyI GACNNNGTC 1 cut(s) 527
RsaI GTAC 1 cut(s) 576
RsaNI GTAC 1 cut(s) 575
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 729, 834, 1021
SatI GCNGC 6 cut(s) 62, 65, 219, 458, 537, 811
Sau3AI GATC 6 cut(s) 151, 442, 499, 652, 711, 736
Sau96I GGNCC 2 cut(s) 209, 529
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 2 cut(s) 401, 670
ScrFI CCNGG 4 cut(s) 207, 521, 590, 694
SfaNI GCATC 3 cut(s) 172, 624, 940
SfcI CTRYAG 2 cut(s) 399, 889
Sfr274I CTCGAG 1 cut(s) 446
SgrAI CRCCGGYG 1 cut(s) 115
SinI GGWCC 2 cut(s) 209, 529
SlaI CTCGAG 1 cut(s) 446
SmlI CTYRAG 3 cut(s) 290, 446, 722
SmoI CTYRAG 3 cut(s) 290, 446, 722
SphI GCATGC 1 cut(s) 913
Sse9I AATT 3 cut(s) 85, 744, 829
SseBI AGGCCT 1 cut(s) 887
SsiI CCGC 3 cut(s) 119, 167, 457
StuI AGGCCT 1 cut(s) 887
StyD4I CCNGG 4 cut(s) 205, 519, 588, 692
TaaI ACNGT 2 cut(s) 104, 785
TaiI ACGT 1 cut(s) 881
TaqI TCGA 5 cut(s) 99, 132, 447, 502, 609
TasI AATT 3 cut(s) 85, 744, 829
TatI WGTACW 1 cut(s) 574
TauI GCSGC 1 cut(s) 460
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 3 cut(s) 729, 834, 1021
Tru9I TTAA 3 cut(s) 729, 834, 1021
TscAI CASTG 2 cut(s) 238, 790
TseFI GTSAC 2 cut(s) 233, 779
TseI GCWGC 5 cut(s) 61, 64, 218, 536, 810
Tsp45I GTSAC 2 cut(s) 233, 779
TspDTI ATGAA 3 cut(s) 176, 717, 915
TspRI CASTG 2 cut(s) 238, 790
Tth111I GACNNNGTC 1 cut(s) 527
VpaK11BI GGWCC 2 cut(s) 209, 529
XagI CCTNNNNNAGG 1 cut(s) 524
XapI RAATTY 1 cut(s) 85
XceI RCATGY 1 cut(s) 913
XhoI CTCGAG 1 cut(s) 446
XmiI GTMKAC 2 cut(s) 398, 1014
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.