AT1G53023

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006585
Physical Location & Seq
Reverse (-)
19754214 .. 19755843
1630 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53023.1

Sequence Viewer

Length: 951 bp
ATGGTGGAGATAAAGGATTCTTCCAATGTTAGGAAACTTAAGGAAGAGTTTCTGCGAGACTTTAAAAGATTTGACACCGTTGAAGATTTCTCAGATCATCACTATGCTGCTGAGGGGAGTCCGGCTAAACTTTCGCTTTGGCACCTAAAATTTAAAGGGCGGCCGAAGAATTGGGTGAAAGATATTCAAAAAGAGTGGAAGATTCTTGACAAGAATTTGCCAGAGACAATATTTGTCAGAGCATGTGAATCAAGAATTGATCTTTTGAGAGCTGTAATTATTGGAGCCGAGGGAACTCCTTACCATGACGGTCTCTTCTTTTTCGATATTCAGTTCCCAGATACCTATCCTTCAGTGCCACCAAAAGTTCATTACCATTCGGGCGGGCTTAGAATCAACCCGAATCTATACAAATGTGGTAAAGTGTGCTTGAGTCTTATCAGTACCTGGACCGGTAAAAAGAGGGAGAAATGGCTCCCCAAGGAGTCCACAATGTTGCAGCTTCTTGTCTCAATCCAAGCACTAATCTTGAATGAAAAACCATATTATAACGAGCCTGGCTACGAGAAATCCATGGGGACTCCATTAGGCGAGTCCTATTCTAAAGATTATAGTGAGAACGTATTCGTATTTTCGTTAAAGACTATGCATTTTGAAGAGTTTGTTCGTAGCCATTTCTTCGTCCGGTCTCACGACATAGTGAAAGCGTGCAATGCTTATAAAGATGGAGCTCCTGTTGGATCAATAGATAAAGGCGGAGTCAAAAAACAAACTAGACAACGAGGCTCTTTGAAGTTTAGGATCAATGTAACTAGCTTTATGAAGACAGTGGTTGATGAATTCGTCAACTTAGGAGCAATAAGAGAAGCAAATCATAGAGGTGAGCCAAACCCAACATTGTTTTCTTGTTTTTTTTTTTGTTATCTTGAATTGATTATTTGTAGTGTTTAA

Protein Analysis

316

Amino Acids

36.49

Weight (kDa)

8.97

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 62 - 208 1.5e-22 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 549, 720
AccB1I GGYRCC 1 cut(s) 141
AciI CCGC 3 cut(s) 160, 384, 756
AclWI GGATC 2 cut(s) 748, 809
AcoI YGGCCR 1 cut(s) 161
AcsI RAATTY 3 cut(s) 149, 214, 839
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 1 cut(s) 445
AfiI CCNNNNNNNGG 1 cut(s) 30
AflII CTTAAG 1 cut(s) 38
AgeI ACCGGT 1 cut(s) 452
AgsI TTSAA 6 cut(s) 83, 188, 532, 656, 793, 929
AjnI CCWGG 2 cut(s) 446, 556
AjuI GAANNNNNNNTTGG 2 cut(s) 886, 918
AluBI AGCT 4 cut(s) 272, 502, 731, 816
AluI AGCT 4 cut(s) 272, 502, 731, 816
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 5 cut(s) 51, 218, 317, 514, 693
AlwI GGATC 2 cut(s) 748, 809
AlwNI CAGNNNCTG 1 cut(s) 447
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 2 cut(s) 107, 499
ApoI RAATTY 3 cut(s) 149, 214, 839
AsiGI ACCGGT 1 cut(s) 452
Asp700I GAANNNNTTC 2 cut(s) 48, 623
AspS9I GGNCC 1 cut(s) 450
AsuHPI GGTGA 2 cut(s) 187, 893
AvaII GGWCC 1 cut(s) 450
BanI GGYRCC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 733
BbsI GAAGAC 1 cut(s) 830
Bbv12I GWGCWC 1 cut(s) 733
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 94, 511
BccI CCATC 1 cut(s) 719
BciT130I CCWGG 2 cut(s) 448, 558
BcoDI GTCTC 5 cut(s) 51, 218, 317, 514, 693
BfaI CTAG 2 cut(s) 774, 813
BfrI CTTAAG 1 cut(s) 38
BisI GCNGC 3 cut(s) 108, 161, 500
BlsI GCNGC 3 cut(s) 109, 162, 501
Bme1390I CCNGG 2 cut(s) 448, 558
Bme18I GGWCC 1 cut(s) 450
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 3 cut(s) 143, 286, 476
BmrFI CCNGG 2 cut(s) 448, 558
BpiI GAAGAC 1 cut(s) 830
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 451
BsaBI GATNNNNATC 1 cut(s) 345
BsaI GGTCTC 2 cut(s) 317, 693
BsaJI CCNNGG 3 cut(s) 288, 480, 573
BsaWI WCCGGW 2 cut(s) 452, 684
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse118I RCCGGY 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 718
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 2 cut(s) 448, 558
BseDI CCNNGG 3 cut(s) 288, 480, 573
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMI GCAATG 1 cut(s) 718
BseMII CTCAG 2 cut(s) 102, 105
BseX3I CGGCCG 1 cut(s) 161
BseXI GCAGC 2 cut(s) 94, 511
Bsh1285I CGRYCG 1 cut(s) 164
BshFI GGCC 1 cut(s) 163
BshNI GGYRCC 1 cut(s) 141
BshTI ACCGGT 1 cut(s) 452
BsiEI CGRYCG 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 733
BsiSI CCGG 3 cut(s) 122, 453, 685
BslFI GGGAC 1 cut(s) 592
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 5 cut(s) 51, 218, 317, 514, 693
BsmFI GGGAC 1 cut(s) 592
BsnI GGCC 1 cut(s) 163
Bso31I GGTCTC 2 cut(s) 317, 693
Bsp1286I GDGCHC 1 cut(s) 733
Bsp143I GATC 4 cut(s) 94, 259, 740, 801
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 3 cut(s) 160, 384, 756
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 2 cut(s) 103, 104
BspLI GGNNCC 3 cut(s) 143, 286, 476
BspPI GGATC 2 cut(s) 748, 809
BspT107I GGYRCC 1 cut(s) 141
BspTI CTTAAG 1 cut(s) 38
BspTNI GGTCTC 2 cut(s) 317, 693
BsrDI GCAATG 1 cut(s) 718
BsrFI RCCGGY 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 3 cut(s) 288, 480, 573
BssMI GATC 4 cut(s) 94, 259, 740, 801
BssT1I CCWWGG 2 cut(s) 480, 573
Bst2UI CCWGG 2 cut(s) 448, 558
Bst4CI ACNGT 3 cut(s) 79, 311, 829
Bst6I CTCTTC 3 cut(s) 39, 320, 651
BstAFI CTTAAG 1 cut(s) 38
BstC8I GCNNGC 2 cut(s) 386, 709
BstDEI CTNAG 4 cut(s) 91, 111, 389, 850
BstDSI CCRYGG 1 cut(s) 573
BstKTI GATC 4 cut(s) 97, 262, 743, 804
BstMAI GTCTC 5 cut(s) 51, 218, 317, 514, 693
BstMBI GATC 4 cut(s) 94, 259, 740, 801
BstMCI CGRYCG 1 cut(s) 164
BstMWI GCNNNNNNNGC 1 cut(s) 713
BstNI CCWGG 2 cut(s) 448, 558
BstNSI RCATGY 1 cut(s) 246
BstSCI CCNGG 2 cut(s) 446, 556
BstV1I GCAGC 2 cut(s) 94, 511
BstV2I GAAGAC 1 cut(s) 830
BstZI CGGCCG 1 cut(s) 161
BsuRI GGCC 1 cut(s) 163
BtgI CCRYGG 1 cut(s) 573
BtsIMutI CAGTG 2 cut(s) 360, 834
Cac8I GCNNGC 2 cut(s) 386, 709
CaiI CAGNNNCTG 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 1 cut(s) 450
Csp6I GTAC 1 cut(s) 444
CspAI ACCGGT 1 cut(s) 452
CspCI CAANNNNNGTGG 2 cut(s) 176, 211
CviAII CATG 3 cut(s) 243, 305, 574
CviQI GTAC 1 cut(s) 444
DdeI CTNAG 4 cut(s) 91, 111, 389, 850
DpnI GATC 4 cut(s) 96, 261, 742, 803
DpnII GATC 4 cut(s) 94, 259, 740, 801
DraI TTTAAA 2 cut(s) 64, 154
EaeI YGGCCR 1 cut(s) 161
EagI CGGCCG 1 cut(s) 161
Eam1104I CTCTTC 3 cut(s) 39, 320, 651
EarI CTCTTC 3 cut(s) 39, 320, 651
EciI GGCGGA 1 cut(s) 771
Ecl136II GAGCTC 1 cut(s) 731
EclXI CGGCCG 1 cut(s) 161
Eco130I CCWWGG 2 cut(s) 480, 573
Eco24I GRGCYC 1 cut(s) 733
Eco31I GGTCTC 2 cut(s) 317, 693
Eco47I GGWCC 1 cut(s) 450
Eco52I CGGCCG 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 731
Eco57I CTGAAG 1 cut(s) 336
EcoICRI GAGCTC 1 cut(s) 731
EcoRI GAATTC 1 cut(s) 839
EcoRII CCWGG 2 cut(s) 446, 556
EcoT14I CCWWGG 2 cut(s) 480, 573
EcoT22I ATGCAT 1 cut(s) 651
EcoT38I GRGCYC 1 cut(s) 733
ErhI CCWWGG 2 cut(s) 480, 573
FaeI CATG 3 cut(s) 246, 308, 577
FaqI GGGAC 1 cut(s) 592
FatI CATG 3 cut(s) 242, 304, 573
FauI CCCGC 1 cut(s) 377
Fnu4HI GCNGC 3 cut(s) 108, 161, 500
FriOI GRGCYC 1 cut(s) 733
Fsp4HI GCNGC 3 cut(s) 108, 161, 500
FspBI CTAG 2 cut(s) 774, 813
GluI GCNGC 3 cut(s) 108, 161, 500
HaeIII GGCC 1 cut(s) 163
HapII CCGG 3 cut(s) 122, 453, 685
Hin1II CATG 3 cut(s) 246, 308, 577
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HpaII CCGG 3 cut(s) 122, 453, 685
HphI GGTGA 2 cut(s) 187, 893
Hpy166II GTNNAC 2 cut(s) 489, 847
Hpy188I TCNGA 2 cut(s) 94, 239
Hpy188III TCNNGA 5 cut(s) 206, 252, 529, 692, 926
Hpy8I GTNNAC 2 cut(s) 489, 847
HpyAV CCTTC 1 cut(s) 360
HpyCH4III ACNGT 3 cut(s) 79, 311, 829
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 3 cut(s) 499, 649, 711
HpyF10VI GCNNNNNNNGC 1 cut(s) 713
HpyF3I CTNAG 4 cut(s) 91, 111, 389, 850
HpySE526I ACGT 1 cut(s) 621
Hsp92II CATG 3 cut(s) 246, 308, 577
Kzo9I GATC 4 cut(s) 94, 259, 740, 801
LmnI GCTCC 5 cut(s) 284, 480, 728, 736, 854
Lsp1109I GCAGC 2 cut(s) 94, 511
MaeI CTAG 2 cut(s) 774, 813
MaeII ACGT 1 cut(s) 621
MaeIII GTNAC 1 cut(s) 808
MalI GATC 4 cut(s) 96, 261, 742, 803
MboI GATC 4 cut(s) 94, 259, 740, 801
MboII GAAGA 9 cut(s) 12, 56, 95, 178, 211, 307, 668, 670, 835
MhlI GDGCHC 1 cut(s) 733
MluCI AATT 7 cut(s) 149, 169, 214, 255, 276, 839, 929
MlyI GAGTC 6 cut(s) 127, 442, 494, 574, 602, 768
MmeI TCCRAC 1 cut(s) 718
MnlI CCTC 5 cut(s) 106, 283, 456, 776, 872
Mph1103I ATGCAT 1 cut(s) 651
MroXI GAANNNNTTC 2 cut(s) 48, 623
MseI TTAA 5 cut(s) 39, 63, 153, 638, 949
MslI CAYNNNNRTG 2 cut(s) 102, 879
MspCI CTTAAG 1 cut(s) 38
MspI CCGG 3 cut(s) 122, 453, 685
MspR9I CCNGG 2 cut(s) 448, 558
MvaI CCWGG 2 cut(s) 448, 558
MwoI GCNNNNNNNGC 1 cut(s) 713
NcoI CCATGG 1 cut(s) 573
NdeII GATC 4 cut(s) 94, 259, 740, 801
NlaIII CATG 3 cut(s) 246, 308, 577
NlaIV GGNNCC 3 cut(s) 143, 286, 476
NmeAIII GCCGAG 1 cut(s) 313
NsiI ATGCAT 1 cut(s) 651
NspI RCATGY 1 cut(s) 246
PdmI GAANNNNTTC 2 cut(s) 48, 623
PfeI GAWTC 5 cut(s) 17, 202, 248, 393, 403
PinAI ACCGGT 1 cut(s) 452
PkrI GCNGC 3 cut(s) 109, 162, 501
PleI GAGTC 6 cut(s) 126, 441, 493, 574, 601, 767
PpsI GAGTC 6 cut(s) 126, 441, 493, 574, 601, 767
PsiI TTATAA 2 cut(s) 549, 720
Psp124BI GAGCTC 1 cut(s) 733
Psp6I CCWGG 2 cut(s) 446, 556
PspGI CCWGG 2 cut(s) 446, 556
PspN4I GGNNCC 3 cut(s) 143, 286, 476
PspPI GGNCC 1 cut(s) 450
PstNI CAGNNNCTG 1 cut(s) 447
RsaI GTAC 1 cut(s) 445
RsaNI GTAC 1 cut(s) 444
RseI CAYNNNNRTG 2 cut(s) 102, 879
SacI GAGCTC 1 cut(s) 733
SaqAI TTAA 5 cut(s) 39, 63, 153, 638, 949
SatI GCNGC 3 cut(s) 108, 161, 500
Sau3AI GATC 4 cut(s) 94, 259, 740, 801
Sau96I GGNCC 1 cut(s) 450
SchI GAGTC 6 cut(s) 127, 442, 494, 574, 602, 768
ScrFI CCNGG 2 cut(s) 448, 558
SduI GDGCHC 1 cut(s) 733
SetI ASST 9 cut(s) 147, 274, 347, 449, 504, 624, 733, 818, 883
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 2 cut(s) 102, 879
SmlI CTYRAG 2 cut(s) 38, 430
SmoI CTYRAG 2 cut(s) 38, 430
Sse9I AATT 7 cut(s) 149, 169, 214, 255, 276, 839, 929
SsiI CCGC 3 cut(s) 160, 384, 756
SspI AATATT 1 cut(s) 231
SspMI CTAG 2 cut(s) 774, 813
SstI GAGCTC 1 cut(s) 733
StyD4I CCNGG 2 cut(s) 446, 556
StyI CCWWGG 2 cut(s) 480, 573
TaaI ACNGT 3 cut(s) 79, 311, 829
TaiI ACGT 1 cut(s) 624
TaqI TCGA 1 cut(s) 324
TasI AATT 7 cut(s) 149, 169, 214, 255, 276, 839, 929
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 5 cut(s) 17, 202, 248, 393, 403
Tru1I TTAA 5 cut(s) 39, 63, 153, 638, 949
Tru9I TTAA 5 cut(s) 39, 63, 153, 638, 949
TscAI CASTG 2 cut(s) 360, 834
TseI GCWGC 2 cut(s) 107, 499
TspDTI ATGAA 4 cut(s) 359, 549, 836, 852
TspRI CASTG 2 cut(s) 360, 834
Vha464I CTTAAG 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 3 cut(s) 149, 214, 839
XceI RCATGY 1 cut(s) 246
XmnI GAANNNNTTC 2 cut(s) 48, 623
XspI CTAG 2 cut(s) 774, 813
Zsp2I ATGCAT 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.