AT1G53025

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006585
Physical Location & Seq
Reverse (-)
19756445 .. 19759700
3256 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53025.2

Sequence Viewer

Length: 1632 bp
ATGGAGCCTGACGTGGTGGAGATCCCTCCTCCTCCTCTGATCGCTTCGGGTTCTAGAACTCGTAAACCCAGAAAGGCTGTTCCTGAAGTGATTGATGTGGAAAGTTATGAATTCCGCAATGTTGGTGTTGTTAAGGATAACAATGTTGTTGATAAGAAGAACAAAGGGAAGGCAATACAAGTTGACTCCTTTTCTTTCAACAACGTTCAATCTCATCATCATGGATCGTCGCTGCTTAACTTAGAGACGTTCCAAGACTATTATGGTCATAAGAATATCCCTTTTAGTGAATTTGCTAATCAACCAATTGATGTTGATGACTACTCAATGTATCAAGACGTTCTAGATCCTAAGGATGTTCCAGCTGGTGCAGAGGTGACGGTACCTTGGGGTTTAAATTCGAGCAGTAAGGGAACTGCGAAATCAAGTATAAGCATTATGAGAAGTCAATCCATGAAAGGATATGGAACAGTATCTCTGGCAACGACTAATGTTCCTCAATTATGGGATTATACTTTGCCTCAGCAGAATCAGGCTATCTATTCTTCTGTATCGTTTTCTGCGGTTCAACCTCAGACTCCTGATGTAGTGATGGTCACCAATCCTACTCCAAACCCTTTTAGTTATGATGCTTCTGCTAGTTCTTCTCATCCTATAGCGGCTGAGCCTATTTCTTCAGTTCAGGATTCTTCCAATGCTAGGAAACTTAAGGAAGAGTTTCTGAGGGATTTTAAAAGATTTGACACCGTCGAGGATTTCTCAGATCATCACTATGCTTCAAAGGGTAAATCTTCAAAGCAGCACTCGAAGAATTGGGTGAAAAAGGTTCAAGCAGATTGGAAGATTCTTGAGAATGATTTGCCAGAGGCAATATCTGTCAGAGCCTGTGAATCAAGAATGGATCTTTTGAGAGCTGTAATTATTGGAGCTGAGGGAACTCCTTACCATGACGGTCTTTTCTTCTTCGATATTCAGTTCCCAGATACTTATCCTTCAGTGCCACCGAATGTTCATTACCATTCTGGTGGGCTTAGAATCAACCCGAATCTATACAATTGTGGGAAAGTATGCTTGAGTCTTCTCGGTACCTGGGCAGGTAGCGCGAGGGAGAAATGGCTCCCAAATGAGTCGACAATGTTACAGCTTCTTGTCTCAATCCAAGCACTCATCTTGAATGAAAAACCATACTTTAACGAACCTGGCTACGTACAGAGCGCGGGGACTGCATCAGGCGAGTCCAAGTCTAAAGTTTACAGTGAGAACGTATTTCTATTGTCGTTAAAGACTATGGTTTACAGCATAAGAAGACCACCCCAGCATTTTGAAGAGTATGTTCAAAACCATTACTTTGTCCGGTCTCACGACATTGTGAAAGCATGCAATGCTTATAAAGCTGGAGCTCCGCTTGGATCGATGGTTAAAGGCGGAGTCCAAGACCTTGAAGAAGCTAGACAGAGCGGCTCCAAGAAGTTTAAGACCGATGTAGCTAGCTTTATGCAGACAGTGGTTGATGAATTCGTCAAACTAGGAGTCAAGGAGCTTGCAGAAAAACCAGAACCGCCTATGAGCAATGCTAACACAGAGAATCAGAGCAAGAAAAAAACCCGTAAGAGAAGCAGATCATCGAGGTGA

Protein Analysis

543

Amino Acids

60.55

Weight (kDa)

7.68

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 276 - 402 7.7e-21 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1389
Acc36I ACCTGC 1 cut(s) 1085
Acc65I GGTACC 2 cut(s) 382, 1085
AccB1I GGYRCC 2 cut(s) 382, 1085
AccBSI CCGCTC 1 cut(s) 1458
AccI GTMKAC 1 cut(s) 1130
AccII CGCG 2 cut(s) 1103, 1217
AciI CCGC 8 cut(s) 115, 563, 659, 1217, 1403, 1425, 1458, 1559
AclI AACGTT 1 cut(s) 204
AclWI GGATC 5 cut(s) 16, 232, 341, 909, 1417
AcsI RAATTY 4 cut(s) 110, 290, 397, 1514
AcuI CTGAAG 3 cut(s) 105, 660, 978
AfaI GTAC 3 cut(s) 384, 1087, 1209
AfiI CCNNNNNNNGG 1 cut(s) 699
AflII CTTAAG 1 cut(s) 707
AjiI CACGTC 1 cut(s) 13
AjnI CCWGG 2 cut(s) 1088, 1198
Alw21I GWGCWC 1 cut(s) 1402
Alw26I GTCTC 3 cut(s) 239, 1156, 1362
AlwI GGATC 5 cut(s) 16, 232, 341, 909, 1417
AlwNI CAGNNNCTG 1 cut(s) 885
ApeKI GCWGC 2 cut(s) 232, 799
ApoI RAATTY 4 cut(s) 110, 290, 397, 1514
ArsI GACNNNNNNTTYG 2 cut(s) 1515, 1547
Asp700I GAANNNNTTC 1 cut(s) 717
Asp718I GGTACC 2 cut(s) 382, 1085
AspLEI GCGC 2 cut(s) 1103, 1217
AsuHPI GGTGA 3 cut(s) 388, 589, 829
AsuNHI GCTAGC 1 cut(s) 1487
AxyI CCTNAGG 1 cut(s) 351
BanI GGYRCC 2 cut(s) 382, 1085
BanII GRGCYC 1 cut(s) 1402
BbsI GAAGAC 2 cut(s) 1070, 1312
Bbv12I GWGCWC 1 cut(s) 1402
BbvCI CCTCAGC 2 cut(s) 522, 930
BbvI GCAGC 2 cut(s) 219, 811
BccI CCATC 2 cut(s) 586, 1408
BciT130I CCWGG 2 cut(s) 1090, 1200
BcoDI GTCTC 3 cut(s) 239, 1156, 1362
BfaI CTAG 7 cut(s) 54, 344, 639, 699, 1449, 1488, 1526
BfmI CTRYAG 1 cut(s) 654
BfrI CTTAAG 1 cut(s) 707
BfuAI ACCTGC 1 cut(s) 1085
BisI GCNGC 4 cut(s) 233, 660, 800, 1459
BlpI GCTNAGC 1 cut(s) 663
BlsI GCNGC 4 cut(s) 234, 661, 801, 1460
Bme1390I CCNGG 2 cut(s) 1090, 1200
BmgBI CACGTC 1 cut(s) 13
BmiI GGNNCC 5 cut(s) 6, 384, 1087, 1118, 1462
BmrFI CCNGG 2 cut(s) 1090, 1200
BmsI GCATC 2 cut(s) 619, 1235
BmtI GCTAGC 1 cut(s) 1491
BpiI GAAGAC 2 cut(s) 1070, 1312
BplI GAGNNNNNCTC 2 cut(s) 743, 775
BpmI CTGGAG 1 cut(s) 1416
Bpu10I CCTNAGC 2 cut(s) 522, 930
Bpu1102I GCTNAGC 1 cut(s) 663
BpuEI CTTGAG 2 cut(s) 869, 1093
Bsa29I ATCGAT 1 cut(s) 1412
BsaAI YACGTR 1 cut(s) 1207
BsaBI GATNNNNATC 1 cut(s) 987
BsaI GGTCTC 1 cut(s) 1362
BsaJI CCNNGG 2 cut(s) 386, 1089
BsaWI WCCGGW 1 cut(s) 1353
Bsc4I CCNNNNNNNGG 1 cut(s) 699
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 3 cut(s) 124, 1387, 1576
Bse8I GATNNNNATC 1 cut(s) 987
BseBI CCWGG 2 cut(s) 1090, 1200
BseCI ATCGAT 1 cut(s) 1412
BseDI CCNNGG 2 cut(s) 386, 1089
BseGI GGATG 2 cut(s) 361, 649
BseJI GATNNNNATC 1 cut(s) 987
BseLI CCNNNNNNNGG 1 cut(s) 699
BseMI GCAATG 3 cut(s) 124, 1387, 1576
BseMII CTCAG 6 cut(s) 536, 587, 654, 713, 774, 921
BseRI GAGGAG 3 cut(s) 18, 21, 24
BseXI GCAGC 2 cut(s) 219, 811
BseYI CCCAGC 1 cut(s) 1314
BsgI GTGCAG 1 cut(s) 390
Bsh1236I CGCG 2 cut(s) 1103, 1217
BshNI GGYRCC 2 cut(s) 382, 1085
BshVI ATCGAT 1 cut(s) 1412
BsiHKAI GWGCWC 1 cut(s) 1402
BsiSI CCGG 1 cut(s) 1354
BslFI GGGAC 1 cut(s) 1234
BslI CCNNNNNNNGG 1 cut(s) 699
BsmAI GTCTC 3 cut(s) 239, 1156, 1362
BsmBI CGTCTC 1 cut(s) 239
BsmFI GGGAC 1 cut(s) 1234
Bso31I GGTCTC 1 cut(s) 1362
Bsp1286I GDGCHC 1 cut(s) 1402
Bsp143I GATC 8 cut(s) 21, 39, 224, 346, 763, 901, 1409, 1619
Bsp1720I GCTNAGC 1 cut(s) 663
BspACI CCGC 8 cut(s) 115, 563, 659, 1217, 1403, 1425, 1458, 1559
BspCNI CTCAG 6 cut(s) 535, 586, 655, 714, 773, 922
BspDI ATCGAT 1 cut(s) 1412
BspFNI CGCG 2 cut(s) 1103, 1217
BspLI GGNNCC 5 cut(s) 6, 384, 1087, 1118, 1462
BspMI ACCTGC 1 cut(s) 1085
BspOI GCTAGC 1 cut(s) 1491
BspPI GGATC 5 cut(s) 16, 232, 341, 909, 1417
BspT107I GGYRCC 2 cut(s) 382, 1085
BspTI CTTAAG 1 cut(s) 707
BspTNI GGTCTC 1 cut(s) 1362
BsrBI CCGCTC 1 cut(s) 1458
BsrDI GCAATG 3 cut(s) 124, 1387, 1576
BssECI CCNNGG 2 cut(s) 386, 1089
BssMI GATC 8 cut(s) 21, 39, 224, 346, 763, 901, 1409, 1619
BssT1I CCWWGG 1 cut(s) 386
Bst2UI CCWGG 2 cut(s) 1090, 1200
Bst4CI ACNGT 6 cut(s) 382, 472, 748, 953, 1256, 1504
Bst6I CTCTTC 2 cut(s) 708, 1320
BstAFI CTTAAG 1 cut(s) 707
BstAPI GCANNNNNTGC 1 cut(s) 1382
BstBAI YACGTR 1 cut(s) 1207
BstC8I GCNNGC 3 cut(s) 1378, 1489, 1542
BstDEI CTNAG 9 cut(s) 241, 351, 522, 573, 663, 722, 760, 930, 1031
BstEII GGTNACC 1 cut(s) 595
BstF5I GGATG 2 cut(s) 361, 649
BstFNI CGCG 2 cut(s) 1103, 1217
BstHHI GCGC 2 cut(s) 1103, 1217
BstKTI GATC 8 cut(s) 24, 42, 227, 349, 766, 904, 1412, 1622
BstMAI GTCTC 3 cut(s) 239, 1156, 1362
BstMBI GATC 8 cut(s) 21, 39, 224, 346, 763, 901, 1409, 1619
BstMWI GCNNNNNNNGC 4 cut(s) 1100, 1223, 1382, 1391
BstNI CCWGG 2 cut(s) 1090, 1200
BstNSI RCATGY 1 cut(s) 1380
BstPI GGTNACC 1 cut(s) 595
BstSCI CCNGG 2 cut(s) 1088, 1198
BstSFI CTRYAG 1 cut(s) 654
BstSNI TACGTA 1 cut(s) 1207
BstUI CGCG 2 cut(s) 1103, 1217
BstV1I GCAGC 2 cut(s) 219, 811
BstV2I GAAGAC 2 cut(s) 1070, 1312
BstX2I RGATCY 3 cut(s) 21, 346, 901
BstXI CCANNNNNNTGG 1 cut(s) 1025
BstYI RGATCY 3 cut(s) 21, 346, 901
Bsu15I ATCGAT 1 cut(s) 1412
Bsu36I CCTNAGG 1 cut(s) 351
BsuTUI ATCGAT 1 cut(s) 1412
BtrI CACGTC 1 cut(s) 13
BtsCI GGATG 2 cut(s) 361, 649
BtsIMutI CAGTG 3 cut(s) 1002, 1261, 1509
BveI ACCTGC 1 cut(s) 1085
Cac8I GCNNGC 3 cut(s) 1378, 1489, 1542
CaiI CAGNNNCTG 1 cut(s) 885
CfoI GCGC 2 cut(s) 1103, 1217
ClaI ATCGAT 1 cut(s) 1412
Csp6I GTAC 3 cut(s) 383, 1086, 1208
CviAII CATG 4 cut(s) 221, 454, 947, 1377
CviQI GTAC 3 cut(s) 383, 1086, 1208
DdeI CTNAG 9 cut(s) 241, 351, 522, 573, 663, 722, 760, 930, 1031
DpnI GATC 8 cut(s) 23, 41, 226, 348, 765, 903, 1411, 1621
DpnII GATC 8 cut(s) 21, 39, 224, 346, 763, 901, 1409, 1619
DraI TTTAAA 2 cut(s) 396, 733
Eam1104I CTCTTC 2 cut(s) 708, 1320
EarI CTCTTC 2 cut(s) 708, 1320
EciI GGCGGA 1 cut(s) 1440
Ecl136II GAGCTC 1 cut(s) 1400
Eco105I TACGTA 1 cut(s) 1207
Eco130I CCWWGG 1 cut(s) 386
Eco24I GRGCYC 1 cut(s) 1402
Eco31I GGTCTC 1 cut(s) 1362
Eco53kI GAGCTC 1 cut(s) 1400
Eco57I CTGAAG 3 cut(s) 105, 660, 978
Eco81I CCTNAGG 1 cut(s) 351
Eco91I GGTNACC 1 cut(s) 595
EcoICRI GAGCTC 1 cut(s) 1400
EcoO65I GGTNACC 1 cut(s) 595
EcoRI GAATTC 2 cut(s) 110, 1514
EcoRII CCWGG 2 cut(s) 1088, 1198
EcoT14I CCWWGG 1 cut(s) 386
EcoT38I GRGCYC 1 cut(s) 1402
ErhI CCWWGG 1 cut(s) 386
Esp3I CGTCTC 1 cut(s) 239
FaeI CATG 4 cut(s) 224, 457, 950, 1380
FaqI GGGAC 1 cut(s) 1234
FatI CATG 4 cut(s) 220, 453, 946, 1376
FauI CCCGC 1 cut(s) 1210
FblI GTMKAC 1 cut(s) 1130
Fnu4HI GCNGC 4 cut(s) 233, 660, 800, 1459
FokI GGATG 2 cut(s) 368, 636
FriOI GRGCYC 1 cut(s) 1402
Fsp4HI GCNGC 4 cut(s) 233, 660, 800, 1459
FspBI CTAG 7 cut(s) 54, 344, 639, 699, 1449, 1488, 1526
GlaI GCGC 2 cut(s) 1102, 1216
GluI GCNGC 4 cut(s) 233, 660, 800, 1459
GsaI CCCAGC 1 cut(s) 1318
GsuI CTGGAG 1 cut(s) 1416
HapII CCGG 1 cut(s) 1354
HhaI GCGC 2 cut(s) 1103, 1217
Hin1II CATG 4 cut(s) 224, 457, 950, 1380
Hin6I GCGC 2 cut(s) 1101, 1215
HinP1I GCGC 2 cut(s) 1101, 1215
HincII GTYRAC 2 cut(s) 184, 1131
HindII GTYRAC 2 cut(s) 184, 1131
HpaII CCGG 1 cut(s) 1354
HphI GGTGA 3 cut(s) 388, 589, 829
Hpy166II GTNNAC 5 cut(s) 65, 184, 1131, 1252, 1294
Hpy188I TCNGA 6 cut(s) 39, 576, 723, 763, 881, 1590
Hpy8I GTNNAC 5 cut(s) 65, 184, 1131, 1252, 1294
Hpy99I CGWCG 2 cut(s) 232, 752
HpyAV CCTTC 2 cut(s) 163, 1002
HpyCH4III ACNGT 6 cut(s) 382, 472, 748, 953, 1256, 1504
HpyCH4IV ACGT 6 cut(s) 12, 204, 248, 339, 1206, 1263
HpyCH4V TGCA 5 cut(s) 371, 1226, 1380, 1498, 1544
HpyF10VI GCNNNNNNNGC 4 cut(s) 1100, 1223, 1382, 1391
HpyF3I CTNAG 9 cut(s) 241, 351, 522, 573, 663, 722, 760, 930, 1031
HpySE526I ACGT 6 cut(s) 12, 204, 248, 339, 1206, 1263
Hsp92II CATG 4 cut(s) 224, 457, 950, 1380
HspAI GCGC 2 cut(s) 1101, 1215
KpnI GGTACC 2 cut(s) 386, 1089
Kzo9I GATC 8 cut(s) 21, 39, 224, 346, 763, 901, 1409, 1619
LmnI GCTCC 7 cut(s) 4, 926, 1122, 1397, 1405, 1466, 1537
Lsp1109I GCAGC 2 cut(s) 219, 811
LweI GCATC 2 cut(s) 619, 1235
MaeI CTAG 7 cut(s) 54, 344, 639, 699, 1449, 1488, 1526
MaeII ACGT 6 cut(s) 12, 204, 248, 339, 1206, 1263
MaeIII GTNAC 3 cut(s) 376, 595, 1137
MalI GATC 8 cut(s) 23, 41, 226, 348, 765, 903, 1411, 1621
MbiI CCGCTC 1 cut(s) 1458
MboI GATC 8 cut(s) 21, 39, 224, 346, 763, 901, 1409, 1619
MfeI CAATTG 2 cut(s) 306, 1054
MflI RGATCY 3 cut(s) 21, 346, 901
MhlI GDGCHC 1 cut(s) 1402
MluCI AATT 9 cut(s) 110, 290, 306, 397, 500, 811, 918, 1054, 1514
MlyI GAGTC 7 cut(s) 179, 571, 1084, 1136, 1244, 1437, 1539
MroXI GAANNNNTTC 1 cut(s) 717
MseI TTAA 9 cut(s) 132, 237, 395, 708, 732, 1191, 1280, 1419, 1473
MslI CAYNNNNRTG 4 cut(s) 219, 771, 1023, 1627
MspA1I CMGCKG 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 707
MspI CCGG 1 cut(s) 1354
MspR9I CCNGG 2 cut(s) 1090, 1200
MunI CAATTG 2 cut(s) 306, 1054
MvaI CCWGG 2 cut(s) 1090, 1200
MvnI CGCG 2 cut(s) 1103, 1217
MwoI GCNNNNNNNGC 4 cut(s) 1100, 1223, 1382, 1391
NdeII GATC 8 cut(s) 21, 39, 224, 346, 763, 901, 1409, 1619
NheI GCTAGC 1 cut(s) 1487
NlaIII CATG 4 cut(s) 224, 457, 950, 1380
NlaIV GGNNCC 5 cut(s) 6, 384, 1087, 1118, 1462
NmuCI GTSAC 2 cut(s) 376, 595
NspI RCATGY 1 cut(s) 1380
PaeI GCATGC 1 cut(s) 1380
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 7 cut(s) 529, 686, 844, 890, 1035, 1045, 1585
PflFI GACNNNGTC 1 cut(s) 746
PkrI GCNGC 4 cut(s) 234, 661, 801, 1460
PleI GAGTC 7 cut(s) 179, 571, 1083, 1135, 1243, 1436, 1538
PpsI GAGTC 7 cut(s) 179, 571, 1083, 1135, 1243, 1436, 1538
Ppu21I YACGTR 1 cut(s) 1207
PsiI TTATAA 1 cut(s) 1389
Psp124BI GAGCTC 1 cut(s) 1402
Psp1406I AACGTT 1 cut(s) 204
Psp6I CCWGG 2 cut(s) 1088, 1198
PspEI GGTNACC 1 cut(s) 595
PspFI CCCAGC 1 cut(s) 1314
PspGI CCWGG 2 cut(s) 1088, 1198
PspN4I GGNNCC 5 cut(s) 6, 384, 1087, 1118, 1462
PstNI CAGNNNCTG 1 cut(s) 885
PsuI RGATCY 3 cut(s) 21, 346, 901
PsyI GACNNNGTC 1 cut(s) 746
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 3 cut(s) 384, 1087, 1209
RsaNI GTAC 3 cut(s) 383, 1086, 1208
RseI CAYNNNNRTG 4 cut(s) 219, 771, 1023, 1627
SacI GAGCTC 1 cut(s) 1402
SalI GTCGAC 1 cut(s) 1129
SaqAI TTAA 9 cut(s) 132, 237, 395, 708, 732, 1191, 1280, 1419, 1473
SatI GCNGC 4 cut(s) 233, 660, 800, 1459
Sau3AI GATC 8 cut(s) 21, 39, 224, 346, 763, 901, 1409, 1619
SchI GAGTC 7 cut(s) 179, 571, 1084, 1136, 1244, 1437, 1539
ScrFI CCNGG 2 cut(s) 1090, 1200
SduI GDGCHC 1 cut(s) 1402
SfaNI GCATC 2 cut(s) 619, 1235
SfcI CTRYAG 1 cut(s) 654
SmiMI CAYNNNNRTG 4 cut(s) 219, 771, 1023, 1627
SmlI CTYRAG 3 cut(s) 707, 848, 1072
SmoI CTYRAG 3 cut(s) 707, 848, 1072
SnaBI TACGTA 1 cut(s) 1207
SphI GCATGC 1 cut(s) 1380
Sse9I AATT 9 cut(s) 110, 290, 306, 397, 500, 811, 918, 1054, 1514
SsiI CCGC 8 cut(s) 115, 563, 659, 1217, 1403, 1425, 1458, 1559
SspMI CTAG 7 cut(s) 54, 344, 639, 699, 1449, 1488, 1526
SstI GAGCTC 1 cut(s) 1402
StyD4I CCNGG 2 cut(s) 1088, 1198
StyI CCWWGG 1 cut(s) 386
TaaI ACNGT 6 cut(s) 382, 472, 748, 953, 1256, 1504
TaiI ACGT 6 cut(s) 15, 207, 251, 342, 1209, 1266
TaqI TCGA 7 cut(s) 401, 750, 806, 966, 1130, 1412, 1625
TaqII GACCGA 1 cut(s) 1493
TasI AATT 9 cut(s) 110, 290, 306, 397, 500, 811, 918, 1054, 1514
TauI GCSGC 2 cut(s) 662, 1461
TfiI GAWTC 7 cut(s) 529, 686, 844, 890, 1035, 1045, 1585
Tru1I TTAA 9 cut(s) 132, 237, 395, 708, 732, 1191, 1280, 1419, 1473
Tru9I TTAA 9 cut(s) 132, 237, 395, 708, 732, 1191, 1280, 1419, 1473
TscAI CASTG 3 cut(s) 1002, 1261, 1509
TseFI GTSAC 2 cut(s) 376, 595
TseI GCWGC 2 cut(s) 232, 799
Tsp45I GTSAC 2 cut(s) 376, 595
TspDTI ATGAA 5 cut(s) 123, 470, 1001, 1191, 1527
TspRI CASTG 3 cut(s) 1002, 1261, 1509
Tth111I GACNNNGTC 1 cut(s) 746
Vha464I CTTAAG 1 cut(s) 707
XapI RAATTY 4 cut(s) 110, 290, 397, 1514
XbaI TCTAGA 2 cut(s) 53, 343
XceI RCATGY 1 cut(s) 1380
XcmI CCANNNNNNNNNTGG 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 1130
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 7 cut(s) 54, 344, 639, 699, 1449, 1488, 1526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.