RLG00000018913

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006585
Physical Location & Seq
Reverse (-)
42724702 .. 42728236
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018913

Sequence Viewer

Length: 1374 bp
ATGGACTGTTCCGTTTCTGAGGGAGGCCTTCAAGCCGCCATCGCCAAGAAACTCAAACAAAATCAGGCTCGTGATCCTGGGAGCTCGTCTAGTTTAGCAGGTTCGGTAGATAGTTTTGAGCCCCTCAAGAGTTCAGGATCAATCAATTTGAATGCTTTCAAAAGTTCCAATGATTCGTCATGTATTGATGACGATGAAATGAACATTGAAGATGATTGTGATGATGATGATGATGAATATGATTTTGATGACAATGATGTATATCTGTCTGAGGACGATTATTTGACTATGGAATCTCAATTTGACAATGTGGATTTGCCTCCTGGGGTGGAAGCTCCACTTCCCTGGTTGAAGGATCCTTCTCCAAGTGAAAACACATCGTCCACTAAGAAGTTAACTATTTCAGATGCACCACCTGTAACTGGTCTACCATCTGCAGCCGGTTCGTCAGCTGCTTCATGCAGCTCAGCCGTTCCTTCTGAGTCAAGTTCTAGTAAGAACGTACAAAACAGCGATGATGATAGTCTCATGCAAAAATTTCAACAATTTAAGCAATTTGATGTTGTCGAGGACTTCTCGGATCATCACTATAGCCGAATGGGATTTTCAGATGAGCAGCCACCCAAGAATTGGGCAAAGAGAATTCAGGAAGAGTGGAAAAGTTTGGAGAAGGACTTACCTGACACTATATTTGTCCGGGTTTATGAAGCAAGAATGGAACTTCTACGGGCAGTGATTATAGGACCTCCAGGCACTCCGTACCACGATGGTCTTTTTGTCTTTGATTGTCTCTTCCCCACTGACTATCCTAATTCACCACCGATGGTTTACTACTATTCCGGTGGTCTTCGGCTAAATCCAAATTTGTATGATTGTGGGAAAGTTTGTCTCAGTCTTTTAGGCACGTGGAGCGGTAGACAGAGAGAAAACTGGATACCTGGGGAATCAACAATGCTACAAGTTTTGGTCTCTATACAAGCTCTTATACTAAATGCAGACCCCTTTTTCAATGAGCCTGGGTATGAGACAAGGTATACTGGAGTAGAAGGGCAAAAAAAATCCAGGAAGTACAGTGAAGAGGTCTTTATTATGTCTCTGAAGACGATGATGTATACACTAAGGAAGCCACCTAAGTATTTTGAGGACTTTGTCTTGGGTCATTTTCGCAGTCGTGCACATAATATTCTGGCAGCATGTAAGGCATATTCTGAGGGTACATTGGTTGGCTCTTCTATTGGAGATGCAGTGCAGAATGGAGTTGAAAGTGGCTCAAGTGAATTTAAGTCATCTGTGGCTAGGATGATGAATATGCTTGTTACATATTTCACCAGAAATGGATCAACAGACTGTGAGCAGTTTCGAGGTGCAGCCTAA

Protein Analysis

458

Amino Acids

50.67

Weight (kDa)

4.51

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 214 - 340 4.4e-22 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 630
AccBSI CCGCTC 1 cut(s) 912
AccI GTMKAC 4 cut(s) 427, 916, 1034, 1112
AciI CCGC 2 cut(s) 36, 912
AclWI GGATC 6 cut(s) 68, 145, 350, 363, 588, 1345
AcsI RAATTY 4 cut(s) 536, 642, 862, 1277
AcuI CTGAAG 1 cut(s) 1118
AcvI CACGTG 1 cut(s) 906
AfaI GTAC 4 cut(s) 504, 761, 1070, 1216
AfiI CCNNNNNNNGG 2 cut(s) 422, 630
AgsI TTSAA 8 cut(s) 32, 151, 160, 209, 352, 542, 1009, 1262
AjnI CCWGG 7 cut(s) 76, 322, 344, 748, 937, 1015, 1061
AluBI AGCT 5 cut(s) 84, 335, 452, 465, 980
AluI AGCT 5 cut(s) 84, 335, 452, 465, 980
Alw21I GWGCWC 2 cut(s) 86, 1177
Alw26I GTCTC 6 cut(s) 530, 794, 893, 973, 1019, 1098
Alw44I GTGCAC 1 cut(s) 1173
AlwI GGATC 6 cut(s) 68, 145, 350, 363, 588, 1345
AoxI GGCC 1 cut(s) 25
ApaLI GTGCAC 1 cut(s) 1173
ApeKI GCWGC 6 cut(s) 437, 452, 462, 616, 1190, 1367
ApoI RAATTY 4 cut(s) 536, 642, 862, 1277
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
Asp700I GAANNNNTTC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 743
AsuC2I CCSGG 1 cut(s) 698
AsuHPI GGTGA 2 cut(s) 807, 1318
AvaII GGWCC 1 cut(s) 743
BaeGI GKGCMC 1 cut(s) 1177
BaeI ACNNNNGTAYC 2 cut(s) 1206, 1239
BamHI GGATCC 1 cut(s) 355
BanII GRGCYC 2 cut(s) 86, 123
BauI CACGAG 1 cut(s) 69
BbrPI CACGTG 1 cut(s) 906
BbsI GAAGAC 2 cut(s) 839, 1106
Bbv12I GWGCWC 2 cut(s) 86, 1177
BbvI GCAGC 5 cut(s) 439, 449, 474, 628, 1202
BccI CCATC 4 cut(s) 47, 439, 761, 817
BceAI ACGGC 1 cut(s) 455
BcgI CGANNNNNNTGC 2 cut(s) 426, 460
BciT130I CCWGG 7 cut(s) 78, 324, 346, 750, 939, 1017, 1063
BciVI GTATCC 1 cut(s) 927
BcnI CCSGG 1 cut(s) 698
BcoDI GTCTC 6 cut(s) 530, 794, 893, 973, 1019, 1098
BfaI CTAG 3 cut(s) 90, 492, 1296
BfmI CTRYAG 2 cut(s) 435, 589
BfuAI ACCTGC 1 cut(s) 89
BfuI GTATCC 1 cut(s) 927
BisI GCNGC 7 cut(s) 36, 438, 453, 463, 617, 1191, 1368
BlpI GCTNAGC 1 cut(s) 466
BlsI GCNGC 7 cut(s) 37, 439, 454, 464, 618, 1192, 1369
Bme1390I CCNGG 8 cut(s) 78, 324, 346, 698, 750, 939, 1017, 1063
Bme18I GGWCC 1 cut(s) 743
BmgT120I GGNCC 1 cut(s) 743
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 8 cut(s) 78, 324, 346, 698, 750, 939, 1017, 1063
BmsI GCATC 2 cut(s) 397, 1231
BpiI GAAGAC 2 cut(s) 839, 1106
BplI GAGNNNNNCTC 2 cut(s) 560, 592
BpmI CTGGAG 2 cut(s) 732, 1059
Bpu1102I GCTNAGC 1 cut(s) 466
BpuEI CTTGAG 2 cut(s) 110, 1255
BpuMI CCSGG 1 cut(s) 698
BsaAI YACGTR 1 cut(s) 906
BsaBI GATNNNNATC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 973
BsaJI CCNNGG 5 cut(s) 77, 323, 344, 938, 1016
BsaWI WCCGGW 1 cut(s) 839
BsaXI ACNNNNNCTCC 2 cut(s) 1230, 1260
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 630
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 3 cut(s) 427, 935, 1042
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 7 cut(s) 78, 324, 346, 750, 939, 1017, 1063
BseDI CCNNGG 5 cut(s) 77, 323, 344, 938, 1016
BseGI GGATG 1 cut(s) 1305
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 2 cut(s) 422, 630
BseMII CTCAG 6 cut(s) 9, 261, 471, 480, 904, 1200
BseNI ACTGG 3 cut(s) 427, 935, 1042
BseSI GKGCMC 1 cut(s) 1177
BseXI GCAGC 5 cut(s) 439, 449, 474, 628, 1202
BsgI GTGCAG 1 cut(s) 1268
BshFI GGCC 1 cut(s) 27
BsiHKAI GWGCWC 2 cut(s) 86, 1177
BsiSI CCGG 3 cut(s) 441, 697, 840
BslI CCNNNNNNNGG 2 cut(s) 422, 630
BsmAI GTCTC 6 cut(s) 530, 794, 893, 973, 1019, 1098
BsmI GAATGC 1 cut(s) 157
BsnI GGCC 1 cut(s) 27
Bso31I GGTCTC 1 cut(s) 973
Bsp1286I GDGCHC 3 cut(s) 86, 123, 1177
Bsp143I GATC 5 cut(s) 73, 137, 355, 580, 1337
Bsp1720I GCTNAGC 1 cut(s) 466
BspACI CCGC 2 cut(s) 36, 912
BspANI GGCC 1 cut(s) 27
BspCNI CTCAG 6 cut(s) 10, 262, 472, 479, 903, 1201
BspLI GGNNCC 1 cut(s) 357
BspMAI CTGCAG 1 cut(s) 439
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 6 cut(s) 68, 145, 350, 363, 588, 1345
BspQI GCTCTTC 1 cut(s) 1234
BspTNI GGTCTC 1 cut(s) 973
BsrBI CCGCTC 1 cut(s) 912
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 3 cut(s) 427, 935, 1042
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 5 cut(s) 77, 323, 344, 938, 1016
BssMI GATC 5 cut(s) 73, 137, 355, 580, 1337
BssNAI GTATAC 2 cut(s) 1035, 1113
BssSI CACGAG 1 cut(s) 69
Bst1107I GTATAC 2 cut(s) 1035, 1113
Bst2BI CACGAG 1 cut(s) 69
Bst2UI CCWGG 7 cut(s) 78, 324, 346, 750, 939, 1017, 1063
Bst4CI ACNGT 3 cut(s) 8, 1073, 1349
Bst6I CTCTTC 4 cut(s) 645, 797, 1071, 1234
BstBAI YACGTR 1 cut(s) 906
BstDEI CTNAG 9 cut(s) 18, 270, 387, 466, 480, 890, 1118, 1131, 1209
BstF5I GGATG 1 cut(s) 1305
BstKTI GATC 5 cut(s) 76, 140, 358, 583, 1340
BstMAI GTCTC 6 cut(s) 530, 794, 893, 973, 1019, 1098
BstMBI GATC 5 cut(s) 73, 137, 355, 580, 1337
BstMWI GCNNNNNNNGC 3 cut(s) 41, 909, 1199
BstNI CCWGG 7 cut(s) 78, 324, 346, 750, 939, 1017, 1063
BstNSI RCATGY 1 cut(s) 1197
BstSCI CCNGG 8 cut(s) 76, 322, 344, 696, 748, 937, 1015, 1061
BstSFI CTRYAG 2 cut(s) 435, 589
BstSLI GKGCMC 1 cut(s) 1177
BstV1I GCAGC 5 cut(s) 439, 449, 474, 628, 1202
BstV2I GAAGAC 2 cut(s) 839, 1106
BstX2I RGATCY 1 cut(s) 355
BstXI CCANNNNNNTGG 1 cut(s) 345
BstYI RGATCY 1 cut(s) 355
BstZ17I GTATAC 2 cut(s) 1035, 1113
BsuI GTATCC 1 cut(s) 927
BsuRI GGCC 1 cut(s) 27
BtgZI GCGATG 2 cut(s) 25, 528
BtsCI GGATG 1 cut(s) 1305
BtsI GCAGTG 2 cut(s) 738, 1251
BtsIMutI CAGTG 4 cut(s) 738, 798, 1078, 1251
BveI ACCTGC 1 cut(s) 89
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 1 cut(s) 743
Csp6I GTAC 4 cut(s) 503, 760, 1069, 1215
CviAII CATG 4 cut(s) 180, 459, 529, 1194
CviQI GTAC 4 cut(s) 503, 760, 1069, 1215
DdeI CTNAG 9 cut(s) 18, 270, 387, 466, 480, 890, 1118, 1131, 1209
DpnI GATC 5 cut(s) 75, 139, 357, 582, 1339
DpnII GATC 5 cut(s) 73, 137, 355, 580, 1337
Eam1104I CTCTTC 4 cut(s) 645, 797, 1071, 1234
EarI CTCTTC 4 cut(s) 645, 797, 1071, 1234
Ecl136II GAGCTC 1 cut(s) 84
Eco147I AGGCCT 1 cut(s) 27
Eco24I GRGCYC 2 cut(s) 86, 123
Eco31I GGTCTC 1 cut(s) 973
Eco47I GGWCC 1 cut(s) 743
Eco53kI GAGCTC 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 1118
Eco72I CACGTG 1 cut(s) 906
EcoICRI GAGCTC 1 cut(s) 84
EcoO109I RGGNCCY 1 cut(s) 743
EcoRI GAATTC 1 cut(s) 642
EcoRII CCWGG 7 cut(s) 76, 322, 344, 748, 937, 1015, 1061
EcoT38I GRGCYC 2 cut(s) 86, 123
FaeI CATG 4 cut(s) 183, 462, 532, 1197
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FatI CATG 4 cut(s) 179, 458, 528, 1193
FblI GTMKAC 4 cut(s) 427, 916, 1034, 1112
Fnu4HI GCNGC 7 cut(s) 36, 438, 453, 463, 617, 1191, 1368
FokI GGATG 1 cut(s) 1312
FriOI GRGCYC 2 cut(s) 86, 123
Fsp4HI GCNGC 7 cut(s) 36, 438, 453, 463, 617, 1191, 1368
FspBI CTAG 3 cut(s) 90, 492, 1296
GluI GCNGC 7 cut(s) 36, 438, 453, 463, 617, 1191, 1368
GsuI CTGGAG 2 cut(s) 732, 1059
HaeIII GGCC 1 cut(s) 27
HapII CCGG 3 cut(s) 441, 697, 840
Hin1II CATG 4 cut(s) 183, 462, 532, 1197
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 4 cut(s) 173, 293, 482, 944
HpaI GTTAAC 1 cut(s) 396
HpaII CCGG 3 cut(s) 441, 697, 840
HphI GGTGA 2 cut(s) 807, 1318
Hpy166II GTNNAC 8 cut(s) 384, 396, 428, 829, 917, 1035, 1113, 1175
Hpy188I TCNGA 8 cut(s) 19, 271, 406, 481, 580, 610, 1098, 1210
Hpy188III TCNNGA 4 cut(s) 71, 127, 135, 647
Hpy8I GTNNAC 8 cut(s) 384, 396, 428, 829, 917, 1035, 1113, 1175
HpyAV CCTTC 6 cut(s) 38, 346, 369, 486, 664, 1040
HpyCH4III ACNGT 3 cut(s) 8, 1073, 1349
HpyCH4IV ACGT 2 cut(s) 501, 905
HpyCH4V TGCA 9 cut(s) 410, 437, 462, 532, 995, 1175, 1244, 1249, 1367
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 909, 1199
HpyF3I CTNAG 9 cut(s) 18, 270, 387, 466, 480, 890, 1118, 1131, 1209
HpySE526I ACGT 2 cut(s) 501, 905
Hsp92II CATG 4 cut(s) 183, 462, 532, 1197
KspAI GTTAAC 1 cut(s) 396
Kzo9I GATC 5 cut(s) 73, 137, 355, 580, 1337
LguI GCTCTTC 1 cut(s) 1234
LmnI GCTCC 3 cut(s) 81, 340, 909
Lsp1109I GCAGC 5 cut(s) 439, 449, 474, 628, 1202
LweI GCATC 2 cut(s) 397, 1231
MaeI CTAG 3 cut(s) 90, 492, 1296
MaeII ACGT 2 cut(s) 501, 905
MaeIII GTNAC 2 cut(s) 418, 1315
MalI GATC 5 cut(s) 75, 139, 357, 582, 1339
MbiI CCGCTC 1 cut(s) 912
MboI GATC 5 cut(s) 73, 137, 355, 580, 1337
MboII GAAGA 7 cut(s) 221, 662, 784, 839, 1088, 1111, 1221
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 3 cut(s) 86, 123, 1177
MlyI GAGTC 1 cut(s) 491
MroXI GAANNNNTTC 1 cut(s) 155
MseI TTAA 3 cut(s) 395, 549, 1281
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 3 cut(s) 441, 697, 840
MspR9I CCNGG 8 cut(s) 78, 324, 346, 698, 750, 939, 1017, 1063
Mva1269I GAATGC 1 cut(s) 157
MvaI CCWGG 7 cut(s) 78, 324, 346, 750, 939, 1017, 1063
MwoI GCNNNNNNNGC 3 cut(s) 41, 909, 1199
NciI CCSGG 1 cut(s) 698
NdeII GATC 5 cut(s) 73, 137, 355, 580, 1337
NlaIII CATG 4 cut(s) 183, 462, 532, 1197
NlaIV GGNNCC 1 cut(s) 357
NspI RCATGY 1 cut(s) 1197
PceI AGGCCT 1 cut(s) 27
PciSI GCTCTTC 1 cut(s) 1234
PctI GAATGC 1 cut(s) 157
PdmI GAANNNNTTC 1 cut(s) 155
PfeI GAWTC 3 cut(s) 173, 293, 944
PflFI GACNNNGTC 1 cut(s) 1148
PflMI CCANNNNNTGG 1 cut(s) 630
PfoI TCCNGGA 1 cut(s) 1061
PkrI GCNGC 7 cut(s) 37, 439, 454, 464, 618, 1192, 1369
PleI GAGTC 1 cut(s) 490
PmaCI CACGTG 1 cut(s) 906
PmlI CACGTG 1 cut(s) 906
PpsI GAGTC 1 cut(s) 490
Ppu21I YACGTR 1 cut(s) 906
PpuMI RGGWCCY 1 cut(s) 743
Psp124BI GAGCTC 1 cut(s) 86
Psp5II RGGWCCY 1 cut(s) 743
Psp6I CCWGG 7 cut(s) 76, 322, 344, 748, 937, 1015, 1061
PspCI CACGTG 1 cut(s) 906
PspGI CCWGG 7 cut(s) 76, 322, 344, 748, 937, 1015, 1061
PspN4I GGNNCC 1 cut(s) 357
PspPI GGNCC 1 cut(s) 743
PspPPI RGGWCCY 1 cut(s) 743
PstI CTGCAG 1 cut(s) 439
PsuI RGATCY 1 cut(s) 355
PsyI GACNNNGTC 1 cut(s) 1148
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 4 cut(s) 504, 761, 1070, 1216
RsaNI GTAC 4 cut(s) 503, 760, 1069, 1215
SacI GAGCTC 1 cut(s) 86
SapI GCTCTTC 1 cut(s) 1234
SaqAI TTAA 3 cut(s) 395, 549, 1281
SatI GCNGC 7 cut(s) 36, 438, 453, 463, 617, 1191, 1368
Sau3AI GATC 5 cut(s) 73, 137, 355, 580, 1337
Sau96I GGNCC 1 cut(s) 743
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 8 cut(s) 78, 324, 346, 698, 750, 939, 1017, 1063
SduI GDGCHC 3 cut(s) 86, 123, 1177
SfaNI GCATC 2 cut(s) 397, 1231
SfcI CTRYAG 2 cut(s) 435, 589
SinI GGWCC 1 cut(s) 743
SmlI CTYRAG 2 cut(s) 125, 1270
SmoI CTYRAG 2 cut(s) 125, 1270
SseBI AGGCCT 1 cut(s) 27
SsiI CCGC 2 cut(s) 36, 912
SspI AATATT 1 cut(s) 1183
SspMI CTAG 3 cut(s) 90, 492, 1296
SstI GAGCTC 1 cut(s) 86
StuI AGGCCT 1 cut(s) 27
StyD4I CCNGG 8 cut(s) 76, 322, 344, 696, 748, 937, 1015, 1061
TaaI ACNGT 3 cut(s) 8, 1073, 1349
TaiI ACGT 2 cut(s) 504, 908
TaqI TCGA 2 cut(s) 567, 1360
TatI WGTACW 1 cut(s) 1068
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 3 cut(s) 173, 293, 944
Tru1I TTAA 3 cut(s) 395, 549, 1281
Tru9I TTAA 3 cut(s) 395, 549, 1281
TscAI CASTG 4 cut(s) 738, 805, 1078, 1251
TseI GCWGC 6 cut(s) 437, 452, 462, 616, 1190, 1367
TspDTI ATGAA 6 cut(s) 210, 215, 249, 447, 720, 1319
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 4 cut(s) 738, 805, 1078, 1251
Tth111I GACNNNGTC 1 cut(s) 1148
Van91I CCANNNNNTGG 1 cut(s) 630
VneI GTGCAC 1 cut(s) 1173
VpaK11BI GGWCC 1 cut(s) 743
XapI RAATTY 4 cut(s) 536, 642, 862, 1277
XceI RCATGY 1 cut(s) 1197
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
XmiI GTMKAC 4 cut(s) 427, 916, 1034, 1112
XmnI GAANNNNTTC 1 cut(s) 155
XspI CTAG 3 cut(s) 90, 492, 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.