Rh2CG309000

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0006585
Physical Location & Seq
Reverse (-)
38775961 .. 38780052
4092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG309000.1

Sequence Viewer

Length: 1185 bp
ATGAAGATTGAAGATGATCGTGATGATGATGATGGTGATGACGATGATTATGATTTTGATGACAATGATGTATATCTGTCTGATGACGATTATTTGACCATGGAATCTCAATTTGATAATGTGGATTTGCCTCCTGGGGTGGAAGCTTCACTTCCCTGGTTGAAGGATCCTTCTCCAAGTGAAAACACATCATCCACTAAGAAGTTAACTATTTCAGATGCACCACCTGTAACTGGTCTACCATCTGCAGCCGGTTCGTCATCTGCTTCATGCAGCTCAGCTGTTCCTTCTGAGTCAAGTTCTAGTACGAACGTACAAAACAGCGATGACGATAGTCTCATGCAAAAATTTCAACAGTTTAAGCAATTTGATGTTGTCGAGGACTTCTCGGATCATCACTATAGCCGAATGGGATTTTCAGATGAGCAGCCACCCAAGAATTGGGCCAAGAGAATTCAGGAAGAGTGGAAAATTTTGGAGAAGGACTTACCTGACACTATATTTGTCCGGGTTTATGAAGCAAGAATGGAACTTCTACGGGCAGTGATTATAGGACCTCCAGGCACTCCGTACCATGATGGTCTTTTTGTCTTTGATTGTCTCTTCCCCACTGACTATCCTAATTCACCACCGATGGTTTACTACTATTCCGGTGGTCTTCGGCTAAATCCAAATTTGTATGATTGTGGGAAAGTTTGTCTCAGTCTTTTAGGCACGTGGAGCGGTAGACAGAGAGAAAACTGGATACCTGGGGAATCAACAATGCTACAAGTTTTGGTCTCTATACAAGCTCTTATACTGAATGCAGACCCCTTTTTCAATGAGCCTGGGTATGAGACAAGGTATACTGGATTAGAAGGGCAAAAAAAATCCAGGAAGTACAGTGAAGAGGTCTTTATTATGTCTCTGAAGACGATGATGTATACACTAAGGAAGCCACCTAAGTATTTTGAGGACTTTGTCTTGGGTCATTTTCGCAATCGTGCACATAATATTCTGGCAGCATGTAAGGCATATTCTGAGGGTACATTGGTTGGGTCTTCTATTGGAGATGCAGTGCAGAATGGAGTTGAAAGTGGCTCAAGTGAATTTAAGTCATCTGTTGCTAGGATGATGAATATGCTTGTTACATATTTCACCAGAAATGGATCAACAGACTGTGAGCAGTTTCGAGGTGCAGCCTAA

Protein Analysis

394

Amino Acids

44.26

Weight (kDa)

4.51

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 151 - 280 9e-22 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 441
AccBSI CCGCTC 1 cut(s) 723
AccI GTMKAC 4 cut(s) 238, 727, 845, 923
AciI CCGC 1 cut(s) 723
AclWI GGATC 4 cut(s) 161, 174, 399, 1156
AcsI RAATTY 5 cut(s) 347, 453, 471, 673, 1088
AcuI CTGAAG 1 cut(s) 929
AcvI CACGTG 1 cut(s) 717
AfaI GTAC 5 cut(s) 307, 315, 572, 881, 1027
AfiI CCNNNNNNNGG 2 cut(s) 233, 441
AgsI TTSAA 5 cut(s) 11, 163, 353, 820, 1073
AjnI CCWGG 6 cut(s) 133, 155, 559, 748, 826, 872
AluBI AGCT 4 cut(s) 146, 276, 281, 791
AluI AGCT 4 cut(s) 146, 276, 281, 791
Alw21I GWGCWC 1 cut(s) 988
Alw26I GTCTC 6 cut(s) 341, 605, 704, 784, 830, 909
Alw44I GTGCAC 1 cut(s) 984
AlwI GGATC 4 cut(s) 161, 174, 399, 1156
AoxI GGCC 1 cut(s) 444
ApaLI GTGCAC 1 cut(s) 984
ApeKI GCWGC 5 cut(s) 248, 273, 427, 1001, 1178
ApoI RAATTY 5 cut(s) 347, 453, 471, 673, 1088
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AspS9I GGNCC 2 cut(s) 444, 554
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 3 cut(s) 47, 618, 1129
AvaII GGWCC 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 988
BaeI ACNNNNGTAYC 2 cut(s) 1017, 1050
BamHI GGATCC 1 cut(s) 166
BbrPI CACGTG 1 cut(s) 717
BbsI GAAGAC 3 cut(s) 650, 917, 1032
Bbv12I GWGCWC 1 cut(s) 988
BbvI GCAGC 4 cut(s) 260, 285, 439, 1013
BccI CCATC 4 cut(s) 26, 250, 572, 628
BcgI CGANNNNNNTGC 2 cut(s) 237, 271
BciT130I CCWGG 6 cut(s) 135, 157, 561, 750, 828, 874
BciVI GTATCC 1 cut(s) 738
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 6 cut(s) 341, 605, 704, 784, 830, 909
BfaI CTAG 2 cut(s) 303, 1107
BfmI CTRYAG 2 cut(s) 246, 400
BfuI GTATCC 1 cut(s) 738
BisI GCNGC 5 cut(s) 249, 274, 428, 1002, 1179
BlpI GCTNAGC 1 cut(s) 277
BlsI GCNGC 5 cut(s) 250, 275, 429, 1003, 1180
Bme1390I CCNGG 7 cut(s) 135, 157, 509, 561, 750, 828, 874
Bme18I GGWCC 1 cut(s) 554
BmgT120I GGNCC 2 cut(s) 444, 554
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 7 cut(s) 135, 157, 509, 561, 750, 828, 874
BmsI GCATC 2 cut(s) 208, 1042
BoxI GACNNNNGTC 1 cut(s) 333
BpiI GAAGAC 3 cut(s) 650, 917, 1032
BplI GAGNNNNNCTC 2 cut(s) 371, 403
BpmI CTGGAG 1 cut(s) 543
Bpu1102I GCTNAGC 1 cut(s) 277
BpuEI CTTGAG 1 cut(s) 1066
BpuMI CCSGG 1 cut(s) 509
BsaAI YACGTR 1 cut(s) 717
BsaBI GATNNNNATC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 784
BsaJI CCNNGG 5 cut(s) 99, 134, 155, 749, 827
BsaWI WCCGGW 1 cut(s) 650
BsaXI ACNNNNNCTCC 2 cut(s) 1041, 1071
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 441
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 3 cut(s) 238, 746, 853
Bse8I GATNNNNATC 1 cut(s) 72
BseBI CCWGG 6 cut(s) 135, 157, 561, 750, 828, 874
BseDI CCNNGG 5 cut(s) 99, 134, 155, 749, 827
BseGI GGATG 2 cut(s) 191, 1116
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 2 cut(s) 233, 441
BseMII CTCAG 4 cut(s) 282, 291, 715, 1011
BseNI ACTGG 3 cut(s) 238, 746, 853
BseSI GKGCMC 1 cut(s) 988
BseXI GCAGC 4 cut(s) 260, 285, 439, 1013
BsgI GTGCAG 1 cut(s) 1079
BshFI GGCC 1 cut(s) 446
BsiHKAI GWGCWC 1 cut(s) 988
BsiSI CCGG 3 cut(s) 252, 508, 651
BslI CCNNNNNNNGG 2 cut(s) 233, 441
BsmAI GTCTC 6 cut(s) 341, 605, 704, 784, 830, 909
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 1 cut(s) 446
Bso31I GGTCTC 1 cut(s) 784
Bsp1286I GDGCHC 1 cut(s) 988
Bsp143I GATC 4 cut(s) 16, 166, 391, 1148
Bsp1720I GCTNAGC 1 cut(s) 277
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 1 cut(s) 723
BspANI GGCC 1 cut(s) 446
BspCNI CTCAG 4 cut(s) 283, 290, 714, 1012
BspLI GGNNCC 1 cut(s) 168
BspMAI CTGCAG 1 cut(s) 250
BspPI GGATC 4 cut(s) 161, 174, 399, 1156
BspTNI GGTCTC 1 cut(s) 784
BsrBI CCGCTC 1 cut(s) 723
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 3 cut(s) 238, 746, 853
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 5 cut(s) 99, 134, 155, 749, 827
BssMI GATC 4 cut(s) 16, 166, 391, 1148
BssNAI GTATAC 2 cut(s) 846, 924
BssT1I CCWWGG 1 cut(s) 99
Bst1107I GTATAC 2 cut(s) 846, 924
Bst2UI CCWGG 6 cut(s) 135, 157, 561, 750, 828, 874
Bst4CI ACNGT 3 cut(s) 357, 884, 1160
Bst6I CTCTTC 3 cut(s) 456, 608, 882
BstBAI YACGTR 1 cut(s) 717
BstDEI CTNAG 7 cut(s) 198, 277, 291, 701, 929, 942, 1020
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 2 cut(s) 191, 1116
BstKTI GATC 4 cut(s) 19, 169, 394, 1151
BstMAI GTCTC 6 cut(s) 341, 605, 704, 784, 830, 909
BstMBI GATC 4 cut(s) 16, 166, 391, 1148
BstMWI GCNNNNNNNGC 2 cut(s) 720, 1010
BstNI CCWGG 6 cut(s) 135, 157, 561, 750, 828, 874
BstNSI RCATGY 1 cut(s) 1008
BstPAI GACNNNNGTC 1 cut(s) 333
BstSCI CCNGG 7 cut(s) 133, 155, 507, 559, 748, 826, 872
BstSFI CTRYAG 2 cut(s) 246, 400
BstSLI GKGCMC 1 cut(s) 988
BstV1I GCAGC 4 cut(s) 260, 285, 439, 1013
BstV2I GAAGAC 3 cut(s) 650, 917, 1032
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BstZ17I GTATAC 2 cut(s) 846, 924
BsuI GTATCC 1 cut(s) 738
BsuRI GGCC 1 cut(s) 446
BtgI CCRYGG 1 cut(s) 99
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 191, 1116
BtsI GCAGTG 2 cut(s) 549, 1062
BtsIMutI CAGTG 4 cut(s) 549, 609, 889, 1062
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 2 cut(s) 444, 554
Csp6I GTAC 5 cut(s) 306, 314, 571, 880, 1026
CviAII CATG 5 cut(s) 100, 270, 340, 575, 1005
CviQI GTAC 5 cut(s) 306, 314, 571, 880, 1026
DdeI CTNAG 7 cut(s) 198, 277, 291, 701, 929, 942, 1020
DpnI GATC 4 cut(s) 18, 168, 393, 1150
DpnII GATC 4 cut(s) 16, 166, 391, 1148
Eam1104I CTCTTC 3 cut(s) 456, 608, 882
EarI CTCTTC 3 cut(s) 456, 608, 882
Eco130I CCWWGG 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 784
Eco47I GGWCC 1 cut(s) 554
Eco57I CTGAAG 1 cut(s) 929
Eco72I CACGTG 1 cut(s) 717
EcoO109I RGGNCCY 1 cut(s) 554
EcoRI GAATTC 1 cut(s) 453
EcoRII CCWGG 6 cut(s) 133, 155, 559, 748, 826, 872
EcoT14I CCWWGG 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 5 cut(s) 103, 273, 343, 578, 1008
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FatI CATG 5 cut(s) 99, 269, 339, 574, 1004
FblI GTMKAC 4 cut(s) 238, 727, 845, 923
Fnu4HI GCNGC 5 cut(s) 249, 274, 428, 1002, 1179
FokI GGATG 2 cut(s) 178, 1123
Fsp4HI GCNGC 5 cut(s) 249, 274, 428, 1002, 1179
FspBI CTAG 2 cut(s) 303, 1107
GluI GCNGC 5 cut(s) 249, 274, 428, 1002, 1179
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 1 cut(s) 446
HapII CCGG 3 cut(s) 252, 508, 651
Hin1II CATG 5 cut(s) 103, 273, 343, 578, 1008
HincII GTYRAC 1 cut(s) 207
HindII GTYRAC 1 cut(s) 207
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 3 cut(s) 104, 293, 755
HpaI GTTAAC 1 cut(s) 207
HpaII CCGG 3 cut(s) 252, 508, 651
HphI GGTGA 3 cut(s) 47, 618, 1129
Hpy166II GTNNAC 7 cut(s) 207, 239, 640, 728, 846, 924, 986
Hpy188I TCNGA 7 cut(s) 82, 217, 292, 391, 421, 909, 1021
Hpy188III TCNNGA 2 cut(s) 20, 458
Hpy8I GTNNAC 7 cut(s) 207, 239, 640, 728, 846, 924, 986
HpyAV CCTTC 5 cut(s) 157, 180, 297, 475, 851
HpyCH4III ACNGT 3 cut(s) 357, 884, 1160
HpyCH4IV ACGT 2 cut(s) 312, 716
HpyCH4V TGCA 9 cut(s) 221, 248, 273, 343, 806, 986, 1055, 1060, 1178
HpyF10VI GCNNNNNNNGC 2 cut(s) 720, 1010
HpyF3I CTNAG 7 cut(s) 198, 277, 291, 701, 929, 942, 1020
HpySE526I ACGT 2 cut(s) 312, 716
Hsp92II CATG 5 cut(s) 103, 273, 343, 578, 1008
KspAI GTTAAC 1 cut(s) 207
Kzo9I GATC 4 cut(s) 16, 166, 391, 1148
LmnI GCTCC 1 cut(s) 720
Lsp1109I GCAGC 4 cut(s) 260, 285, 439, 1013
LweI GCATC 2 cut(s) 208, 1042
MaeI CTAG 2 cut(s) 303, 1107
MaeII ACGT 2 cut(s) 312, 716
MaeIII GTNAC 2 cut(s) 229, 1126
MalI GATC 4 cut(s) 18, 168, 393, 1150
MbiI CCGCTC 1 cut(s) 723
MboI GATC 4 cut(s) 16, 166, 391, 1148
MboII GAAGA 8 cut(s) 16, 23, 473, 595, 650, 899, 922, 1032
MflI RGATCY 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 988
MluCI AATT 9 cut(s) 110, 347, 365, 439, 453, 471, 622, 673, 1088
MlyI GAGTC 1 cut(s) 302
MnlI CCTC 7 cut(s) 141, 373, 567, 883, 946, 1015, 1166
MseI TTAA 3 cut(s) 206, 360, 1092
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 3 cut(s) 252, 508, 651
MspR9I CCNGG 7 cut(s) 135, 157, 509, 561, 750, 828, 874
Mva1269I GAATGC 1 cut(s) 808
MvaI CCWGG 6 cut(s) 135, 157, 561, 750, 828, 874
MwoI GCNNNNNNNGC 2 cut(s) 720, 1010
NciI CCSGG 1 cut(s) 509
NcoI CCATGG 1 cut(s) 99
NdeII GATC 4 cut(s) 16, 166, 391, 1148
NlaIII CATG 5 cut(s) 103, 273, 343, 578, 1008
NlaIV GGNNCC 1 cut(s) 168
NspI RCATGY 1 cut(s) 1008
PctI GAATGC 1 cut(s) 808
PfeI GAWTC 2 cut(s) 104, 755
PflFI GACNNNGTC 1 cut(s) 959
PflMI CCANNNNNTGG 1 cut(s) 441
PfoI TCCNGGA 1 cut(s) 872
PkrI GCNGC 5 cut(s) 250, 275, 429, 1003, 1180
PleI GAGTC 1 cut(s) 301
PmaCI CACGTG 1 cut(s) 717
PmlI CACGTG 1 cut(s) 717
PpsI GAGTC 1 cut(s) 301
Ppu21I YACGTR 1 cut(s) 717
PpuMI RGGWCCY 1 cut(s) 554
PshAI GACNNNNGTC 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 554
Psp6I CCWGG 6 cut(s) 133, 155, 559, 748, 826, 872
PspCI CACGTG 1 cut(s) 717
PspGI CCWGG 6 cut(s) 133, 155, 559, 748, 826, 872
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 2 cut(s) 444, 554
PspPPI RGGWCCY 1 cut(s) 554
PstI CTGCAG 1 cut(s) 250
PsuI RGATCY 1 cut(s) 166
PsyI GACNNNGTC 1 cut(s) 959
PvuII CAGCTG 1 cut(s) 281
RsaI GTAC 5 cut(s) 307, 315, 572, 881, 1027
RsaNI GTAC 5 cut(s) 306, 314, 571, 880, 1026
SaqAI TTAA 3 cut(s) 206, 360, 1092
SatI GCNGC 5 cut(s) 249, 274, 428, 1002, 1179
Sau3AI GATC 4 cut(s) 16, 166, 391, 1148
Sau96I GGNCC 2 cut(s) 444, 554
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 7 cut(s) 135, 157, 509, 561, 750, 828, 874
SduI GDGCHC 1 cut(s) 988
SfaNI GCATC 2 cut(s) 208, 1042
SfcI CTRYAG 2 cut(s) 246, 400
SinI GGWCC 1 cut(s) 554
SmlI CTYRAG 1 cut(s) 1081
SmoI CTYRAG 1 cut(s) 1081
Sse9I AATT 9 cut(s) 110, 347, 365, 439, 453, 471, 622, 673, 1088
SsiI CCGC 1 cut(s) 723
SspI AATATT 1 cut(s) 994
SspMI CTAG 2 cut(s) 303, 1107
StyD4I CCNGG 7 cut(s) 133, 155, 507, 559, 748, 826, 872
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 3 cut(s) 357, 884, 1160
TaiI ACGT 2 cut(s) 315, 719
TaqI TCGA 2 cut(s) 378, 1171
TasI AATT 9 cut(s) 110, 347, 365, 439, 453, 471, 622, 673, 1088
TatI WGTACW 1 cut(s) 879
TfiI GAWTC 2 cut(s) 104, 755
Tru1I TTAA 3 cut(s) 206, 360, 1092
Tru9I TTAA 3 cut(s) 206, 360, 1092
TscAI CASTG 4 cut(s) 549, 616, 889, 1062
TseI GCWGC 5 cut(s) 248, 273, 427, 1001, 1178
TspDTI ATGAA 4 cut(s) 17, 258, 531, 1130
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 4 cut(s) 549, 616, 889, 1062
Tth111I GACNNNGTC 1 cut(s) 959
Van91I CCANNNNNTGG 1 cut(s) 441
VneI GTGCAC 1 cut(s) 984
VpaK11BI GGWCC 1 cut(s) 554
XapI RAATTY 5 cut(s) 347, 453, 471, 673, 1088
XceI RCATGY 1 cut(s) 1008
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
XmiI GTMKAC 4 cut(s) 238, 727, 845, 923
XspI CTAG 2 cut(s) 303, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.