Rroxscaffold_2G00117080

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006585
Physical Location & Seq
Forward (+)
44537760 .. 44542263
4504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00117080.1

Sequence Viewer

Length: 1143 bp
ATGATTGGGCACCTTGGAAGAGCACTGCCTTTGCAAGCCGCCACGTTTCTATCCTCTGCAAACAGGCTTGGAATCGTGGATCTCGACATGGACTGTTCCGTTTCTGAGGGAGGCCTTCAAGCCGCCATCGCCAAGAAACTCAAACAAAATCAGGCCCGTGATCCTGGCAGCTCGTCTAGTTTAGCAGGTTCAGTAGATAGTTTTGAGCCCCTCAAGAGTTCAGGATCAATCAATTCGAATGCTCTCAAAAATTCCAATGATTCGTCATGTATTGATGACGATGAAGTGAACATTGAAGATGATTGTGATGATGATGAATATGATTTTGATGACAATGATGTATATCTGTCTGAGGATGATTATTTGACTATGGAATCTCAATTTGACAATGTGGATTTGCCTCCTGGGGTGGAAGCTCCACTTCCCTGGTTGAAGGATCCTTCTCCAAGTGAAAACACATCATCCACTAAGAAGTTAACTATTTCAGATGCACCACCTGTAACTGGTCTACCATCTGCAGCTGGTTCGTCATCTGCTTCATGCAGCTCAGCTGTTCCTTCTGAGTCAAGTTCTAGTAAGAACGTACAAAACAGCGATGACGATAGTCTCATGCAAAAATTTCAACAATTTAAGCAATTTGATGTTGTCGAGGACTTCTCAGATCATCACTATAGCCGAATGGGATTTTCAGATGAGCAGCCACCCAAGAATTGGGCAAAGAGAATTCAGGAAGAGTGGAAAATTTTGGAGAAGGACTTACCTGACACTATATTTGTCCGGGTTTATGAAGCAAGAATGGAACTTCTACGGGCAGTGATTATAGGACCTCCAGGCACTCCGTACCATGATGGTCTTTTTGTCTTTGATTGTCTCTTCCCCACTGACTATCCTAATTCACCACCGTATTTTGAGGACTTTGTCTTGGGTCATTTTCGCAATCGTGCACATAATATTCTGGCAGCGTGTAAGGCATATTCTGAGGGTACATTGGTTGGGTCTTCTATTGGAGATGCAGTGCAGAATGGAGTTGAAACTGGCTCAAGTGAATTTAAGTCATCTGTGGCTAGGATGATGAATATGCTTGTTACATATTTCACCAGAAATGGATCAACAGACTGTGAGCAGTTTCGAGGTGCAGCCTAA

Protein Analysis

380

Amino Acids

41.51

Weight (kDa)

4.36

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 241 - 303 2.9e-10 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 9
AccB7I CCANNNNNTGG 1 cut(s) 711
AccI GTMKAC 1 cut(s) 508
AciI CCGC 2 cut(s) 39, 123
AclWI GGATC 6 cut(s) 87, 155, 232, 431, 444, 1114
AcsI RAATTY 5 cut(s) 250, 617, 723, 741, 1046
AfaI GTAC 3 cut(s) 585, 842, 985
AfiI CCNNNNNNNGG 2 cut(s) 503, 711
AgsI TTSAA 5 cut(s) 119, 296, 433, 623, 1031
AjnI CCWGG 4 cut(s) 163, 403, 425, 829
AluBI AGCT 5 cut(s) 171, 416, 521, 546, 551
AluI AGCT 5 cut(s) 171, 416, 521, 546, 551
Alw21I GWGCWC 2 cut(s) 25, 946
Alw26I GTCTC 2 cut(s) 611, 875
Alw44I GTGCAC 1 cut(s) 942
AlwI GGATC 6 cut(s) 87, 155, 232, 431, 444, 1114
AoxI GGCC 2 cut(s) 112, 153
ApaLI GTGCAC 1 cut(s) 942
ApeKI GCWGC 6 cut(s) 168, 518, 543, 697, 959, 1136
ApoI RAATTY 5 cut(s) 250, 617, 723, 741, 1046
ArsI GACNNNNNNTTYG 2 cut(s) 755, 787
AspS9I GGNCC 2 cut(s) 154, 824
AsuC2I CCSGG 1 cut(s) 779
AsuHPI GGTGA 2 cut(s) 888, 1087
AsuII TTCGAA 1 cut(s) 236
AvaII GGWCC 1 cut(s) 824
BaeGI GKGCMC 2 cut(s) 12, 946
BaeI ACNNNNGTAYC 2 cut(s) 975, 1008
BamHI GGATCC 1 cut(s) 436
BanI GGYRCC 1 cut(s) 9
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 2 cut(s) 25, 946
BbvI GCAGC 5 cut(s) 180, 530, 555, 709, 971
BccI CCATC 3 cut(s) 134, 520, 842
BcgI CGANNNNNNTGC 2 cut(s) 507, 541
BciT130I CCWGG 4 cut(s) 165, 405, 427, 831
BcnI CCSGG 1 cut(s) 779
BcoDI GTCTC 2 cut(s) 611, 875
BfaI CTAG 3 cut(s) 177, 573, 1065
BfmI CTRYAG 2 cut(s) 516, 670
BfuAI ACCTGC 1 cut(s) 176
BisI GCNGC 8 cut(s) 39, 123, 169, 519, 544, 698, 960, 1137
BlpI GCTNAGC 1 cut(s) 547
BlsI GCNGC 8 cut(s) 40, 124, 170, 520, 545, 699, 961, 1138
Bme1390I CCNGG 5 cut(s) 165, 405, 427, 779, 831
Bme18I GGWCC 1 cut(s) 824
BmgT120I GGNCC 2 cut(s) 154, 824
BmiI GGNNCC 2 cut(s) 11, 438
BmrFI CCNGG 5 cut(s) 165, 405, 427, 779, 831
BmsI GCATC 2 cut(s) 478, 1000
BoxI GACNNNNGTC 1 cut(s) 603
BpiI GAAGAC 1 cut(s) 990
BplI GAGNNNNNCTC 2 cut(s) 641, 673
BpmI CTGGAG 1 cut(s) 813
Bpu1102I GCTNAGC 1 cut(s) 547
Bpu14I TTCGAA 1 cut(s) 236
BpuEI CTTGAG 2 cut(s) 197, 1024
BpuMI CCSGG 1 cut(s) 779
BsaBI GATNNNNATC 1 cut(s) 342
BsaJI CCNNGG 3 cut(s) 13, 404, 425
BsaXI ACNNNNNCTCC 2 cut(s) 999, 1029
Bsc4I CCNNNNNNNGG 2 cut(s) 503, 711
Bse1I ACTGG 2 cut(s) 508, 1039
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 4 cut(s) 165, 405, 427, 831
BseDI CCNNGG 3 cut(s) 13, 404, 425
BseGI GGATG 3 cut(s) 361, 461, 1074
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 503, 711
BseMII CTCAG 6 cut(s) 96, 342, 552, 561, 672, 969
BseNI ACTGG 2 cut(s) 508, 1039
BseSI GKGCMC 2 cut(s) 12, 946
BseXI GCAGC 5 cut(s) 180, 530, 555, 709, 971
BsgI GTGCAG 1 cut(s) 1037
BshFI GGCC 2 cut(s) 114, 155
BshNI GGYRCC 1 cut(s) 9
BsiHKAI GWGCWC 2 cut(s) 25, 946
BsiSI CCGG 1 cut(s) 778
BslI CCNNNNNNNGG 2 cut(s) 503, 711
BsmAI GTCTC 2 cut(s) 611, 875
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 2 cut(s) 114, 155
Bsp119I TTCGAA 1 cut(s) 236
Bsp1286I GDGCHC 4 cut(s) 12, 25, 210, 946
Bsp143I GATC 6 cut(s) 79, 160, 224, 436, 661, 1106
Bsp1720I GCTNAGC 1 cut(s) 547
BspACI CCGC 2 cut(s) 39, 123
BspANI GGCC 2 cut(s) 114, 155
BspCNI CTCAG 6 cut(s) 97, 343, 553, 560, 671, 970
BspLI GGNNCC 2 cut(s) 11, 438
BspMAI CTGCAG 1 cut(s) 520
BspMI ACCTGC 1 cut(s) 176
BspPI GGATC 6 cut(s) 87, 155, 232, 431, 444, 1114
BspQI GCTCTTC 1 cut(s) 13
BspT104I TTCGAA 1 cut(s) 236
BspT107I GGYRCC 1 cut(s) 9
BsrI ACTGG 2 cut(s) 508, 1039
BssECI CCNNGG 3 cut(s) 13, 404, 425
BssMI GATC 6 cut(s) 79, 160, 224, 436, 661, 1106
BssT1I CCWWGG 1 cut(s) 13
Bst2UI CCWGG 4 cut(s) 165, 405, 427, 831
Bst4CI ACNGT 3 cut(s) 95, 903, 1118
Bst6I CTCTTC 3 cut(s) 13, 726, 878
BstBI TTCGAA 1 cut(s) 236
BstC8I GCNNGC 1 cut(s) 36
BstDEI CTNAG 7 cut(s) 105, 351, 468, 547, 561, 658, 978
BstF5I GGATG 3 cut(s) 361, 461, 1074
BstKTI GATC 6 cut(s) 82, 163, 227, 439, 664, 1109
BstMAI GTCTC 2 cut(s) 611, 875
BstMBI GATC 6 cut(s) 79, 160, 224, 436, 661, 1106
BstMWI GCNNNNNNNGC 2 cut(s) 128, 968
BstNI CCWGG 4 cut(s) 165, 405, 427, 831
BstPAI GACNNNNGTC 1 cut(s) 603
BstSCI CCNGG 5 cut(s) 163, 403, 425, 777, 829
BstSFI CTRYAG 2 cut(s) 516, 670
BstSLI GKGCMC 2 cut(s) 12, 946
BstV1I GCAGC 5 cut(s) 180, 530, 555, 709, 971
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 2 cut(s) 79, 436
BstXI CCANNNNNNTGG 1 cut(s) 426
BstYI RGATCY 2 cut(s) 79, 436
BsuRI GGCC 2 cut(s) 114, 155
BtgZI GCGATG 2 cut(s) 112, 609
BtsCI GGATG 3 cut(s) 361, 461, 1074
BtsI GCAGTG 3 cut(s) 23, 819, 1020
BtsIMutI CAGTG 4 cut(s) 23, 819, 879, 1020
BveI ACCTGC 1 cut(s) 176
Cac8I GCNNGC 1 cut(s) 36
Cfr13I GGNCC 2 cut(s) 154, 824
Csp6I GTAC 3 cut(s) 584, 841, 984
CviAII CATG 5 cut(s) 88, 267, 540, 610, 845
CviQI GTAC 3 cut(s) 584, 841, 984
DdeI CTNAG 7 cut(s) 105, 351, 468, 547, 561, 658, 978
DpnI GATC 6 cut(s) 81, 162, 226, 438, 663, 1108
DpnII GATC 6 cut(s) 79, 160, 224, 436, 661, 1106
Eam1104I CTCTTC 3 cut(s) 13, 726, 878
EarI CTCTTC 3 cut(s) 13, 726, 878
Eco130I CCWWGG 1 cut(s) 13
Eco147I AGGCCT 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 824
EcoO109I RGGNCCY 1 cut(s) 824
EcoRI GAATTC 1 cut(s) 723
EcoRII CCWGG 4 cut(s) 163, 403, 425, 829
EcoT14I CCWWGG 1 cut(s) 13
EcoT38I GRGCYC 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 13
FaeI CATG 5 cut(s) 91, 270, 543, 613, 848
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FatI CATG 5 cut(s) 87, 266, 539, 609, 844
FblI GTMKAC 1 cut(s) 508
Fnu4HI GCNGC 8 cut(s) 39, 123, 169, 519, 544, 698, 960, 1137
FokI GGATG 3 cut(s) 368, 448, 1081
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 8 cut(s) 39, 123, 169, 519, 544, 698, 960, 1137
FspBI CTAG 3 cut(s) 177, 573, 1065
GluI GCNGC 8 cut(s) 39, 123, 169, 519, 544, 698, 960, 1137
GsuI CTGGAG 1 cut(s) 813
HaeIII GGCC 2 cut(s) 114, 155
HapII CCGG 1 cut(s) 778
Hin1II CATG 5 cut(s) 91, 270, 543, 613, 848
HincII GTYRAC 1 cut(s) 477
HindII GTYRAC 1 cut(s) 477
HinfI GANTC 4 cut(s) 72, 260, 374, 563
HpaI GTTAAC 1 cut(s) 477
HpaII CCGG 1 cut(s) 778
HphI GGTGA 2 cut(s) 888, 1087
Hpy166II GTNNAC 4 cut(s) 289, 477, 509, 944
Hpy188I TCNGA 7 cut(s) 106, 352, 487, 562, 661, 691, 979
Hpy188III TCNNGA 4 cut(s) 83, 214, 222, 728
Hpy8I GTNNAC 4 cut(s) 289, 477, 509, 944
HpyAV CCTTC 5 cut(s) 125, 427, 450, 567, 745
HpyCH4III ACNGT 3 cut(s) 95, 903, 1118
HpyCH4IV ACGT 2 cut(s) 44, 582
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 968
HpyF3I CTNAG 7 cut(s) 105, 351, 468, 547, 561, 658, 978
HpySE526I ACGT 2 cut(s) 44, 582
Hsp92II CATG 5 cut(s) 91, 270, 543, 613, 848
KspAI GTTAAC 1 cut(s) 477
Kzo9I GATC 6 cut(s) 79, 160, 224, 436, 661, 1106
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 1 cut(s) 421
Lsp1109I GCAGC 5 cut(s) 180, 530, 555, 709, 971
LweI GCATC 2 cut(s) 478, 1000
MaeI CTAG 3 cut(s) 177, 573, 1065
MaeII ACGT 2 cut(s) 44, 582
MaeIII GTNAC 2 cut(s) 499, 1084
MalI GATC 6 cut(s) 81, 162, 226, 438, 663, 1108
MboI GATC 6 cut(s) 79, 160, 224, 436, 661, 1106
MboII GAAGA 5 cut(s) 30, 308, 743, 865, 990
MflI RGATCY 2 cut(s) 79, 436
MhlI GDGCHC 4 cut(s) 12, 25, 210, 946
MlyI GAGTC 1 cut(s) 572
MseI TTAA 3 cut(s) 476, 630, 1050
MspA1I CMGCKG 2 cut(s) 521, 551
MspI CCGG 1 cut(s) 778
MspR9I CCNGG 5 cut(s) 165, 405, 427, 779, 831
Mva1269I GAATGC 1 cut(s) 244
MvaI CCWGG 4 cut(s) 165, 405, 427, 831
MwoI GCNNNNNNNGC 2 cut(s) 128, 968
NciI CCSGG 1 cut(s) 779
NdeII GATC 6 cut(s) 79, 160, 224, 436, 661, 1106
NlaIII CATG 5 cut(s) 91, 270, 543, 613, 848
NlaIV GGNNCC 2 cut(s) 11, 438
NspV TTCGAA 1 cut(s) 236
PceI AGGCCT 1 cut(s) 114
PciSI GCTCTTC 1 cut(s) 13
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 1 cut(s) 244
PfeI GAWTC 3 cut(s) 72, 260, 374
PflFI GACNNNGTC 1 cut(s) 917
PflMI CCANNNNNTGG 1 cut(s) 711
PkrI GCNGC 8 cut(s) 40, 124, 170, 520, 545, 699, 961, 1138
PleI GAGTC 1 cut(s) 571
PpsI GAGTC 1 cut(s) 571
PpuMI RGGWCCY 1 cut(s) 824
PshAI GACNNNNGTC 1 cut(s) 603
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 4 cut(s) 163, 403, 425, 829
PspGI CCWGG 4 cut(s) 163, 403, 425, 829
PspN4I GGNNCC 2 cut(s) 11, 438
PspPI GGNCC 2 cut(s) 154, 824
PspPPI RGGWCCY 1 cut(s) 824
PstI CTGCAG 1 cut(s) 520
PsuI RGATCY 2 cut(s) 79, 436
PsyI GACNNNGTC 1 cut(s) 917
PvuII CAGCTG 2 cut(s) 521, 551
RsaI GTAC 3 cut(s) 585, 842, 985
RsaNI GTAC 3 cut(s) 584, 841, 984
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 3 cut(s) 476, 630, 1050
SatI GCNGC 8 cut(s) 39, 123, 169, 519, 544, 698, 960, 1137
Sau3AI GATC 6 cut(s) 79, 160, 224, 436, 661, 1106
Sau96I GGNCC 2 cut(s) 154, 824
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 5 cut(s) 165, 405, 427, 779, 831
SduI GDGCHC 4 cut(s) 12, 25, 210, 946
SfaNI GCATC 2 cut(s) 478, 1000
SfcI CTRYAG 2 cut(s) 516, 670
SfuI TTCGAA 1 cut(s) 236
SinI GGWCC 1 cut(s) 824
SmlI CTYRAG 2 cut(s) 212, 1039
SmoI CTYRAG 2 cut(s) 212, 1039
SseBI AGGCCT 1 cut(s) 114
SsiI CCGC 2 cut(s) 39, 123
SspI AATATT 1 cut(s) 952
SspMI CTAG 3 cut(s) 177, 573, 1065
StuI AGGCCT 1 cut(s) 114
StyD4I CCNGG 5 cut(s) 163, 403, 425, 777, 829
StyI CCWWGG 1 cut(s) 13
TaaI ACNGT 3 cut(s) 95, 903, 1118
TaiI ACGT 2 cut(s) 47, 585
TaqI TCGA 4 cut(s) 84, 236, 648, 1129
TauI GCSGC 2 cut(s) 41, 125
TfiI GAWTC 3 cut(s) 72, 260, 374
Tru1I TTAA 3 cut(s) 476, 630, 1050
Tru9I TTAA 3 cut(s) 476, 630, 1050
TscAI CASTG 4 cut(s) 30, 819, 886, 1020
TseI GCWGC 6 cut(s) 168, 518, 543, 697, 959, 1136
TspDTI ATGAA 5 cut(s) 297, 330, 528, 801, 1088
TspGWI ACGGA 2 cut(s) 88, 828
TspRI CASTG 4 cut(s) 30, 819, 886, 1020
Tth111I GACNNNGTC 1 cut(s) 917
Van91I CCANNNNNTGG 1 cut(s) 711
VneI GTGCAC 1 cut(s) 942
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 5 cut(s) 250, 617, 723, 741, 1046
XcmI CCANNNNNNNNNTGG 1 cut(s) 708
XmiI GTMKAC 1 cut(s) 508
XspI CTAG 3 cut(s) 177, 573, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.