Rw2G025990

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006585
Physical Location & Seq
Reverse (-)
39824818 .. 39829071
4254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G025990.1

Sequence Viewer

Length: 1380 bp
ATGGACTGTTCCGTTTCTGAGGGAGGCCTTCAAGCCGCCATCGCCAAGAAACTCAAACAAAATCAGGCCCGTGATCCTGGGAGCTCGTCTACTTTAGCAGGTTCAGTAGATAGTTTTGAGCCCCTCAAGAGTTCAGGATCAATCAATTTGAATGCTCTCAAAAGTTCCAATGATTCGTCATGTATTGATGACGATGAAATGAAGATTGAAGATGATCGTGATGATGATGATGACGACGATGATTATGATTTTGATGACAATGATGTATATCTGTCTGATGACGATTATTTGACCATGGAATCTCAATTTGACAATGTGGATTTGCCTCCTGGGGTGGAAGCTTCACTTCCCTGGTTGAAGGATCCTTCTCCAAGTGAAAACACATCGTCCACTAAGAAGTTAACTATTTCAGATGCACCACCTGTAACTGGTCTACCATCTGCAGCCGGTTCGTCATCTGCTTCATGCAGCTCAGCTGTTCCTTCTGAATCAAGTTCTAGTAAGAACGTACAAAACAGCGATGACGATAGTCTCATGCAAAAATTTCAACAATTTAAGCAATTTGATGTTGTCGAGGACTTCTCAGATCATCACTATAGCCGAATGGGATTTTCAGATGAGCAGCCACCCAAGAATTGGGCCAAGAGAATTCAGGAAGAGTGGAAAATTTTGGAGAAGGACTTACCTGACACTATATTTGTCCGGGTTTATGAAGCAAGAATGGAACTTCTACGGGCAGTGATTATAGGACCCCCAGGCACTCCGTACCATGATGGTCTTTTTGTCTTTGATTGTCTCTTCCCAACTGACTATCCTAATTCACCACCGATGGTTTACTACTATTCCGGTGGTCTTCGGCTAAATCCAAATTTGTATGATTGTGGGAAAGTTTGTCTCAGTCTTTTAGGCACGTGGAGCGGTAGACAGAGAGAAAACTGGATACCTGGGGAATCAACAATGCTACAAGTTTTGGTCTCTATACAAGCTCTTATACTGAATGCAGACCCCTTTTTCAATGAGCCTGGGTATGAGACAAGGTATACTGGAGTAGAAGGGCAAAAAAAATCCAGGAAGTACAGTGAAGAGGTCTTTATTATGTCTCTGAAGACGATGATGTATACACTAAGGAAGCCACCTAAGTATTTTGAGGACTTTGTCTTGGGTCATTTTCGCAATCGTGCACATATTATTCTGGCAGCATGTAAGGCATATTCTGAGGGTACATTGGTTGGGTCTTCTATTGGAGATGCAGTGCAGAATGGAGTTGAAAGTGGCTCAAGTGAATTTAAGTCATCTGTGGCTAGGATGATGAATATGCTTGTTACATATTTCACCAGAAATGGATCAACAGACTGTGAGCAGTTTCGAGGTGCAGCCTAA

Protein Analysis

459

Amino Acids

50.98

Weight (kDa)

4.51

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 216 - 342 1.3e-21 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 636
AccBSI CCGCTC 1 cut(s) 918
AccI GTMKAC 5 cut(s) 89, 433, 922, 1040, 1118
AciI CCGC 2 cut(s) 36, 918
AclWI GGATC 5 cut(s) 68, 145, 356, 369, 1351
AcsI RAATTY 5 cut(s) 542, 648, 666, 868, 1283
AcuI CTGAAG 1 cut(s) 1124
AcvI CACGTG 1 cut(s) 912
AfaI GTAC 4 cut(s) 510, 767, 1076, 1222
AfiI CCNNNNNNNGG 2 cut(s) 428, 636
AgsI TTSAA 7 cut(s) 32, 151, 209, 358, 548, 1015, 1268
AjnI CCWGG 7 cut(s) 76, 328, 350, 754, 943, 1021, 1067
AluBI AGCT 5 cut(s) 84, 341, 471, 476, 986
AluI AGCT 5 cut(s) 84, 341, 471, 476, 986
Alw21I GWGCWC 2 cut(s) 86, 1183
Alw26I GTCTC 6 cut(s) 536, 800, 899, 979, 1025, 1104
Alw44I GTGCAC 1 cut(s) 1179
AlwI GGATC 5 cut(s) 68, 145, 356, 369, 1351
AoxI GGCC 3 cut(s) 25, 66, 639
ApaLI GTGCAC 1 cut(s) 1179
ApeKI GCWGC 5 cut(s) 443, 468, 622, 1196, 1373
ApoI RAATTY 5 cut(s) 542, 648, 666, 868, 1283
ArsI GACNNNNNNTTYG 2 cut(s) 680, 712
AspS9I GGNCC 3 cut(s) 67, 639, 749
AsuC2I CCSGG 1 cut(s) 704
AsuHPI GGTGA 2 cut(s) 813, 1324
AvaII GGWCC 1 cut(s) 749
BaeGI GKGCMC 1 cut(s) 1183
BaeI ACNNNNGTAYC 2 cut(s) 1212, 1245
BamHI GGATCC 1 cut(s) 361
BanII GRGCYC 2 cut(s) 86, 123
BbrPI CACGTG 1 cut(s) 912
BbsI GAAGAC 3 cut(s) 845, 1112, 1227
Bbv12I GWGCWC 2 cut(s) 86, 1183
BbvI GCAGC 4 cut(s) 455, 480, 634, 1208
BccI CCATC 4 cut(s) 47, 445, 767, 823
BcgI CGANNNNNNTGC 2 cut(s) 432, 466
BciT130I CCWGG 7 cut(s) 78, 330, 352, 756, 945, 1023, 1069
BciVI GTATCC 1 cut(s) 933
BcnI CCSGG 1 cut(s) 704
BcoDI GTCTC 6 cut(s) 536, 800, 899, 979, 1025, 1104
BfaI CTAG 2 cut(s) 498, 1302
BfmI CTRYAG 2 cut(s) 441, 595
BfuAI ACCTGC 1 cut(s) 89
BfuI GTATCC 1 cut(s) 933
BisI GCNGC 6 cut(s) 36, 444, 469, 623, 1197, 1374
BlpI GCTNAGC 1 cut(s) 472
BlsI GCNGC 6 cut(s) 37, 445, 470, 624, 1198, 1375
Bme1390I CCNGG 8 cut(s) 78, 330, 352, 704, 756, 945, 1023, 1069
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 3 cut(s) 67, 639, 749
BmiI GGNNCC 2 cut(s) 363, 751
BmrFI CCNGG 8 cut(s) 78, 330, 352, 704, 756, 945, 1023, 1069
BmsI GCATC 2 cut(s) 403, 1237
BoxI GACNNNNGTC 1 cut(s) 528
BpiI GAAGAC 3 cut(s) 845, 1112, 1227
BplI GAGNNNNNCTC 2 cut(s) 566, 598
BpmI CTGGAG 1 cut(s) 1065
Bpu1102I GCTNAGC 1 cut(s) 472
BpuEI CTTGAG 2 cut(s) 110, 1261
BpuMI CCSGG 1 cut(s) 704
BsaAI YACGTR 1 cut(s) 912
BsaBI GATNNNNATC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 979
BsaJI CCNNGG 7 cut(s) 77, 294, 329, 350, 754, 944, 1022
BsaWI WCCGGW 1 cut(s) 845
BsaXI ACNNNNNCTCC 2 cut(s) 1236, 1266
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 636
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 3 cut(s) 433, 941, 1048
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 7 cut(s) 78, 330, 352, 756, 945, 1023, 1069
BseDI CCNNGG 7 cut(s) 77, 294, 329, 350, 754, 944, 1022
BseGI GGATG 1 cut(s) 1311
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 2 cut(s) 428, 636
BseMII CTCAG 5 cut(s) 9, 486, 597, 910, 1206
BseNI ACTGG 3 cut(s) 433, 941, 1048
BseSI GKGCMC 1 cut(s) 1183
BseXI GCAGC 4 cut(s) 455, 480, 634, 1208
BsgI GTGCAG 1 cut(s) 1274
BshFI GGCC 3 cut(s) 27, 68, 641
BsiHKAI GWGCWC 2 cut(s) 86, 1183
BsiSI CCGG 3 cut(s) 447, 703, 846
BslI CCNNNNNNNGG 2 cut(s) 428, 636
BsmAI GTCTC 6 cut(s) 536, 800, 899, 979, 1025, 1104
BsmI GAATGC 2 cut(s) 157, 1003
BsnI GGCC 3 cut(s) 27, 68, 641
Bso31I GGTCTC 1 cut(s) 979
Bsp1286I GDGCHC 3 cut(s) 86, 123, 1183
Bsp143I GATC 6 cut(s) 73, 137, 214, 361, 586, 1343
Bsp1720I GCTNAGC 1 cut(s) 472
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 2 cut(s) 36, 918
BspANI GGCC 3 cut(s) 27, 68, 641
BspCNI CTCAG 5 cut(s) 10, 485, 596, 909, 1207
BspLI GGNNCC 2 cut(s) 363, 751
BspMAI CTGCAG 1 cut(s) 445
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 5 cut(s) 68, 145, 356, 369, 1351
BspTNI GGTCTC 1 cut(s) 979
BsrBI CCGCTC 1 cut(s) 918
BsrFI RCCGGY 1 cut(s) 446
BsrI ACTGG 3 cut(s) 433, 941, 1048
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 7 cut(s) 77, 294, 329, 350, 754, 944, 1022
BssMI GATC 6 cut(s) 73, 137, 214, 361, 586, 1343
BssNAI GTATAC 2 cut(s) 1041, 1119
BssT1I CCWWGG 1 cut(s) 294
Bst1107I GTATAC 2 cut(s) 1041, 1119
Bst2UI CCWGG 7 cut(s) 78, 330, 352, 756, 945, 1023, 1069
Bst4CI ACNGT 3 cut(s) 8, 1079, 1355
Bst6I CTCTTC 3 cut(s) 651, 803, 1077
BstBAI YACGTR 1 cut(s) 912
BstDEI CTNAG 8 cut(s) 18, 393, 472, 583, 896, 1124, 1137, 1215
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 1311
BstKTI GATC 6 cut(s) 76, 140, 217, 364, 589, 1346
BstMAI GTCTC 6 cut(s) 536, 800, 899, 979, 1025, 1104
BstMBI GATC 6 cut(s) 73, 137, 214, 361, 586, 1343
BstMWI GCNNNNNNNGC 3 cut(s) 41, 915, 1205
BstNI CCWGG 7 cut(s) 78, 330, 352, 756, 945, 1023, 1069
BstNSI RCATGY 1 cut(s) 1203
BstPAI GACNNNNGTC 1 cut(s) 528
BstSCI CCNGG 8 cut(s) 76, 328, 350, 702, 754, 943, 1021, 1067
BstSFI CTRYAG 2 cut(s) 441, 595
BstSLI GKGCMC 1 cut(s) 1183
BstV1I GCAGC 4 cut(s) 455, 480, 634, 1208
BstV2I GAAGAC 3 cut(s) 845, 1112, 1227
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BstZ17I GTATAC 2 cut(s) 1041, 1119
BsuI GTATCC 1 cut(s) 933
BsuRI GGCC 3 cut(s) 27, 68, 641
BtgI CCRYGG 1 cut(s) 294
BtgZI GCGATG 2 cut(s) 25, 534
BtsCI GGATG 1 cut(s) 1311
BtsI GCAGTG 2 cut(s) 744, 1257
BtsIMutI CAGTG 3 cut(s) 744, 1084, 1257
BveI ACCTGC 1 cut(s) 89
Cfr10I RCCGGY 1 cut(s) 446
Cfr13I GGNCC 3 cut(s) 67, 639, 749
Csp6I GTAC 4 cut(s) 509, 766, 1075, 1221
CviAII CATG 6 cut(s) 180, 295, 465, 535, 770, 1200
CviQI GTAC 4 cut(s) 509, 766, 1075, 1221
DdeI CTNAG 8 cut(s) 18, 393, 472, 583, 896, 1124, 1137, 1215
DpnI GATC 6 cut(s) 75, 139, 216, 363, 588, 1345
DpnII GATC 6 cut(s) 73, 137, 214, 361, 586, 1343
Eam1104I CTCTTC 3 cut(s) 651, 803, 1077
EarI CTCTTC 3 cut(s) 651, 803, 1077
Ecl136II GAGCTC 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 294
Eco147I AGGCCT 1 cut(s) 27
Eco24I GRGCYC 2 cut(s) 86, 123
Eco31I GGTCTC 1 cut(s) 979
Eco47I GGWCC 1 cut(s) 749
Eco53kI GAGCTC 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 1124
Eco72I CACGTG 1 cut(s) 912
EcoICRI GAGCTC 1 cut(s) 84
EcoO109I RGGNCCY 1 cut(s) 749
EcoRI GAATTC 1 cut(s) 648
EcoRII CCWGG 7 cut(s) 76, 328, 350, 754, 943, 1021, 1067
EcoT14I CCWWGG 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 86, 123
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 6 cut(s) 183, 298, 468, 538, 773, 1203
FalI AAGNNNNNCTT 2 cut(s) 330, 362
FatI CATG 6 cut(s) 179, 294, 464, 534, 769, 1199
FblI GTMKAC 5 cut(s) 89, 433, 922, 1040, 1118
Fnu4HI GCNGC 6 cut(s) 36, 444, 469, 623, 1197, 1374
FokI GGATG 1 cut(s) 1318
FriOI GRGCYC 2 cut(s) 86, 123
Fsp4HI GCNGC 6 cut(s) 36, 444, 469, 623, 1197, 1374
FspBI CTAG 2 cut(s) 498, 1302
GluI GCNGC 6 cut(s) 36, 444, 469, 623, 1197, 1374
GsuI CTGGAG 1 cut(s) 1065
HaeIII GGCC 3 cut(s) 27, 68, 641
HapII CCGG 3 cut(s) 447, 703, 846
Hin1II CATG 6 cut(s) 183, 298, 468, 538, 773, 1203
HincII GTYRAC 1 cut(s) 402
HindII GTYRAC 1 cut(s) 402
HindIII AAGCTT 1 cut(s) 339
HinfI GANTC 4 cut(s) 173, 299, 488, 950
HpaI GTTAAC 1 cut(s) 402
HpaII CCGG 3 cut(s) 447, 703, 846
HphI GGTGA 2 cut(s) 813, 1324
Hpy166II GTNNAC 9 cut(s) 90, 390, 402, 434, 835, 923, 1041, 1119, 1181
Hpy188I TCNGA 8 cut(s) 19, 277, 412, 487, 586, 616, 1104, 1216
Hpy188III TCNNGA 4 cut(s) 127, 135, 218, 653
Hpy8I GTNNAC 9 cut(s) 90, 390, 402, 434, 835, 923, 1041, 1119, 1181
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 6 cut(s) 38, 352, 375, 492, 670, 1046
HpyCH4III ACNGT 3 cut(s) 8, 1079, 1355
HpyCH4IV ACGT 2 cut(s) 507, 911
HpyCH4V TGCA 9 cut(s) 416, 443, 468, 538, 1001, 1181, 1250, 1255, 1373
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 915, 1205
HpyF3I CTNAG 8 cut(s) 18, 393, 472, 583, 896, 1124, 1137, 1215
HpySE526I ACGT 2 cut(s) 507, 911
Hsp92II CATG 6 cut(s) 183, 298, 468, 538, 773, 1203
KspAI GTTAAC 1 cut(s) 402
Kzo9I GATC 6 cut(s) 73, 137, 214, 361, 586, 1343
LmnI GCTCC 2 cut(s) 81, 915
Lsp1109I GCAGC 4 cut(s) 455, 480, 634, 1208
LweI GCATC 2 cut(s) 403, 1237
MaeI CTAG 2 cut(s) 498, 1302
MaeII ACGT 2 cut(s) 507, 911
MaeIII GTNAC 2 cut(s) 424, 1321
MalI GATC 6 cut(s) 75, 139, 216, 363, 588, 1345
MbiI CCGCTC 1 cut(s) 918
MboI GATC 6 cut(s) 73, 137, 214, 361, 586, 1343
MboII GAAGA 8 cut(s) 214, 221, 668, 790, 845, 1094, 1117, 1227
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 3 cut(s) 86, 123, 1183
MnlI CCTC 9 cut(s) 13, 17, 134, 336, 568, 1078, 1141, 1210, 1361
MseI TTAA 3 cut(s) 401, 555, 1287
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 3 cut(s) 447, 703, 846
MspR9I CCNGG 8 cut(s) 78, 330, 352, 704, 756, 945, 1023, 1069
Mva1269I GAATGC 2 cut(s) 157, 1003
MvaI CCWGG 7 cut(s) 78, 330, 352, 756, 945, 1023, 1069
MwoI GCNNNNNNNGC 3 cut(s) 41, 915, 1205
NciI CCSGG 1 cut(s) 704
NcoI CCATGG 1 cut(s) 294
NdeII GATC 6 cut(s) 73, 137, 214, 361, 586, 1343
NlaIII CATG 6 cut(s) 183, 298, 468, 538, 773, 1203
NlaIV GGNNCC 2 cut(s) 363, 751
NspI RCATGY 1 cut(s) 1203
PceI AGGCCT 1 cut(s) 27
PctI GAATGC 2 cut(s) 157, 1003
PfeI GAWTC 4 cut(s) 173, 299, 488, 950
PflFI GACNNNGTC 1 cut(s) 1154
PflMI CCANNNNNTGG 1 cut(s) 636
PfoI TCCNGGA 1 cut(s) 1067
PkrI GCNGC 6 cut(s) 37, 445, 470, 624, 1198, 1375
PmaCI CACGTG 1 cut(s) 912
PmlI CACGTG 1 cut(s) 912
Ppu21I YACGTR 1 cut(s) 912
PpuMI RGGWCCY 1 cut(s) 749
PshAI GACNNNNGTC 1 cut(s) 528
Psp124BI GAGCTC 1 cut(s) 86
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 7 cut(s) 76, 328, 350, 754, 943, 1021, 1067
PspCI CACGTG 1 cut(s) 912
PspGI CCWGG 7 cut(s) 76, 328, 350, 754, 943, 1021, 1067
PspN4I GGNNCC 2 cut(s) 363, 751
PspPI GGNCC 3 cut(s) 67, 639, 749
PspPPI RGGWCCY 1 cut(s) 749
PstI CTGCAG 1 cut(s) 445
PsuI RGATCY 1 cut(s) 361
PsyI GACNNNGTC 1 cut(s) 1154
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 4 cut(s) 510, 767, 1076, 1222
RsaNI GTAC 4 cut(s) 509, 766, 1075, 1221
SacI GAGCTC 1 cut(s) 86
SaqAI TTAA 3 cut(s) 401, 555, 1287
SatI GCNGC 6 cut(s) 36, 444, 469, 623, 1197, 1374
Sau3AI GATC 6 cut(s) 73, 137, 214, 361, 586, 1343
Sau96I GGNCC 3 cut(s) 67, 639, 749
ScrFI CCNGG 8 cut(s) 78, 330, 352, 704, 756, 945, 1023, 1069
SduI GDGCHC 3 cut(s) 86, 123, 1183
SfaNI GCATC 2 cut(s) 403, 1237
SfcI CTRYAG 2 cut(s) 441, 595
SinI GGWCC 1 cut(s) 749
SmlI CTYRAG 2 cut(s) 125, 1276
SmoI CTYRAG 2 cut(s) 125, 1276
SseBI AGGCCT 1 cut(s) 27
SsiI CCGC 2 cut(s) 36, 918
SspMI CTAG 2 cut(s) 498, 1302
SstI GAGCTC 1 cut(s) 86
StuI AGGCCT 1 cut(s) 27
StyD4I CCNGG 8 cut(s) 76, 328, 350, 702, 754, 943, 1021, 1067
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 3 cut(s) 8, 1079, 1355
TaiI ACGT 2 cut(s) 510, 914
TaqI TCGA 2 cut(s) 573, 1366
TatI WGTACW 1 cut(s) 1074
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 4 cut(s) 173, 299, 488, 950
Tru1I TTAA 3 cut(s) 401, 555, 1287
Tru9I TTAA 3 cut(s) 401, 555, 1287
TscAI CASTG 3 cut(s) 744, 1084, 1257
TseI GCWGC 5 cut(s) 443, 468, 622, 1196, 1373
TspDTI ATGAA 5 cut(s) 210, 215, 453, 726, 1325
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 3 cut(s) 744, 1084, 1257
Tth111I GACNNNGTC 1 cut(s) 1154
Van91I CCANNNNNTGG 1 cut(s) 636
VneI GTGCAC 1 cut(s) 1179
VpaK11BI GGWCC 1 cut(s) 749
XapI RAATTY 5 cut(s) 542, 648, 666, 868, 1283
XceI RCATGY 1 cut(s) 1203
XcmI CCANNNNNNNNNTGG 1 cut(s) 633
XmiI GTMKAC 5 cut(s) 89, 433, 922, 1040, 1118
XspI CTAG 2 cut(s) 498, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.