Rh2DG348500

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006585
Physical Location & Seq
Reverse (-)
48621474 .. 48624966
3493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG348500.1

Sequence Viewer

Length: 1386 bp
ATGGACTGTTCCGTTTCTGAGGGAGGAGGCCTTCAAGCCGCCATCGCCAAGAAACTCAAACAAAATCAGGCCCGTGATCCTGGGAGCTCGTCTAGTTTAGCAGGTTCAGTAGATAGTTTTGAGCCCCTCAAGAGTTCAGGATCAACCAATTTGAATGCTCTCAAAAGTTCCAATGACTCGTCATGTATTGATGACGATGAAATGAAGATTGAAGATGATCGTGATGATGATGATGGTGATGACGATGATTATGATTTTGATGACAATGATGTATATCTGTCTGATGACGATTATTTGACCATGGAATCTCAATTTGATAATGTGGATTTGCCTCCTGGGGTGGAAGCTTCACTTCCCTGGTTGAAGGATCCTTCTCCAAGTGAAAACACATCATCCACTAAGAAGTTAACTATTTCAGATGCACCACCTGTAACTGGTCTACCATCTGCAGCCGGTTCGTCATCTGCTTCATGCAGCTCAGCTGTTCCTTCTGAGTCAAGTTCTAGTACGAACGTACAAAACAGCGATGACGATAGTCTCATGCAAAAATTTCAACAGTTTAAGCAATTTGATGTTGTCGAGGACTTCTCGGATCATCACTATAGCCGAATGGGATTTTCAGATGAGCAGCCACCCAAGAATTGGGCCAAGAGAATTCAGGAAGAGTGGAAAATTTTGGAGAAGGACTTACCTGACACTATATTTGTCCGGGTTTATGAAGCAAGAATGGAACTTCTACGGGCAGTGATTATAGGACCTCCAGGCACTCCGTACCATGATGGTCTTTTTGTCTTTGATTGTCTCTTCCCCACTGACTATCCTAATTCACCACCGATGGTTTACTACTATTCCGGTGGTCTTCGGCTAAATCCAAATTTGTATGATTGTGGGAAAGTTTGTCTCAGTCTTTTAGGCACGTGGAGCGGTAGACAGAGAGAAAACTGGATACCTGGGGAATCAACAATGCTACAAGTTTTGGTCTCTATACAAGCTCTTATACTGAATGCAGACCCCTTTTTCAATGAGCCTGGGTATGAGACAAGGTATACTGGATTAGAAGGGCAAAAAAAATCCAGGAAGTACAGTGAAGAGGTCTTTATTATGTCTCTGAAGACGATGATGTATACACTAAGGAAGCCACCTAAGTATTTTGAGGACTTTGTCTTGGGTCATTTTCGCAATCGTGCACATAATATTCTGGCAGCATGTAAGGCATATTCTGAGGGTACATTGGTTGGGTCTTCTATTGGAGATGCAGTGCAGAATGGAGTTGAAAGTGGCTCAAGTGAATTTAAGTCATCTGTTGCTAGGATGATGAATATGCTTGTTACATATTTCACCAGAAATGGATCAACAGACTGTGAGCAGTTTCGAGGTGCAGCCTAA

Protein Analysis

461

Amino Acids

51.05

Weight (kDa)

4.48

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 218 - 347 1.2e-21 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 92
AccB7I CCANNNNNTGG 1 cut(s) 642
AccBSI CCGCTC 1 cut(s) 924
AccI GTMKAC 4 cut(s) 439, 928, 1046, 1124
AciI CCGC 2 cut(s) 39, 924
AclWI GGATC 6 cut(s) 71, 148, 362, 375, 600, 1357
AcsI RAATTY 5 cut(s) 548, 654, 672, 874, 1289
AcuI CTGAAG 1 cut(s) 1130
AcvI CACGTG 1 cut(s) 918
AfaI GTAC 5 cut(s) 508, 516, 773, 1082, 1228
AfiI CCNNNNNNNGG 2 cut(s) 434, 642
AgsI TTSAA 7 cut(s) 35, 154, 212, 364, 554, 1021, 1274
AjnI CCWGG 7 cut(s) 79, 334, 356, 760, 949, 1027, 1073
AluBI AGCT 5 cut(s) 87, 347, 477, 482, 992
AluI AGCT 5 cut(s) 87, 347, 477, 482, 992
Alw21I GWGCWC 2 cut(s) 89, 1189
Alw26I GTCTC 6 cut(s) 542, 806, 905, 985, 1031, 1110
Alw44I GTGCAC 1 cut(s) 1185
AlwI GGATC 6 cut(s) 71, 148, 362, 375, 600, 1357
AoxI GGCC 3 cut(s) 28, 69, 645
ApaLI GTGCAC 1 cut(s) 1185
ApeKI GCWGC 5 cut(s) 449, 474, 628, 1202, 1379
ApoI RAATTY 5 cut(s) 548, 654, 672, 874, 1289
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
AspS9I GGNCC 3 cut(s) 70, 645, 755
AsuC2I CCSGG 1 cut(s) 710
AsuHPI GGTGA 3 cut(s) 248, 819, 1330
AvaII GGWCC 1 cut(s) 755
BaeGI GKGCMC 1 cut(s) 1189
BaeI ACNNNNGTAYC 2 cut(s) 1218, 1251
BamHI GGATCC 1 cut(s) 367
BanII GRGCYC 2 cut(s) 89, 126
BbrPI CACGTG 1 cut(s) 918
BbsI GAAGAC 3 cut(s) 851, 1118, 1233
Bbv12I GWGCWC 2 cut(s) 89, 1189
BbvI GCAGC 4 cut(s) 461, 486, 640, 1214
BccI CCATC 5 cut(s) 50, 227, 451, 773, 829
BcgI CGANNNNNNTGC 2 cut(s) 438, 472
BciT130I CCWGG 7 cut(s) 81, 336, 358, 762, 951, 1029, 1075
BciVI GTATCC 1 cut(s) 939
BcnI CCSGG 1 cut(s) 710
BcoDI GTCTC 6 cut(s) 542, 806, 905, 985, 1031, 1110
BfaI CTAG 3 cut(s) 93, 504, 1308
BfmI CTRYAG 2 cut(s) 447, 601
BfuAI ACCTGC 1 cut(s) 92
BfuI GTATCC 1 cut(s) 939
BisI GCNGC 6 cut(s) 39, 450, 475, 629, 1203, 1380
BlpI GCTNAGC 1 cut(s) 478
BlsI GCNGC 6 cut(s) 40, 451, 476, 630, 1204, 1381
Bme1390I CCNGG 8 cut(s) 81, 336, 358, 710, 762, 951, 1029, 1075
Bme18I GGWCC 1 cut(s) 755
BmgT120I GGNCC 3 cut(s) 70, 645, 755
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 8 cut(s) 81, 336, 358, 710, 762, 951, 1029, 1075
BmsI GCATC 2 cut(s) 409, 1243
BoxI GACNNNNGTC 1 cut(s) 534
BpiI GAAGAC 3 cut(s) 851, 1118, 1233
BplI GAGNNNNNCTC 2 cut(s) 572, 604
BpmI CTGGAG 1 cut(s) 744
Bpu1102I GCTNAGC 1 cut(s) 478
BpuEI CTTGAG 2 cut(s) 113, 1267
BpuMI CCSGG 1 cut(s) 710
BsaAI YACGTR 1 cut(s) 918
BsaBI GATNNNNATC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 985
BsaJI CCNNGG 6 cut(s) 80, 300, 335, 356, 950, 1028
BsaWI WCCGGW 1 cut(s) 851
BsaXI ACNNNNNCTCC 2 cut(s) 1242, 1272
Bsc4I CCNNNNNNNGG 2 cut(s) 434, 642
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 3 cut(s) 439, 947, 1054
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 7 cut(s) 81, 336, 358, 762, 951, 1029, 1075
BseDI CCNNGG 6 cut(s) 80, 300, 335, 356, 950, 1028
BseGI GGATG 2 cut(s) 392, 1317
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 434, 642
BseMII CTCAG 5 cut(s) 9, 483, 492, 916, 1212
BseNI ACTGG 3 cut(s) 439, 947, 1054
BseRI GAGGAG 1 cut(s) 39
BseSI GKGCMC 1 cut(s) 1189
BseXI GCAGC 4 cut(s) 461, 486, 640, 1214
BsgI GTGCAG 1 cut(s) 1280
BshFI GGCC 3 cut(s) 30, 71, 647
BsiHKAI GWGCWC 2 cut(s) 89, 1189
BsiSI CCGG 3 cut(s) 453, 709, 852
BslI CCNNNNNNNGG 2 cut(s) 434, 642
BsmAI GTCTC 6 cut(s) 542, 806, 905, 985, 1031, 1110
BsmI GAATGC 2 cut(s) 160, 1009
BsnI GGCC 3 cut(s) 30, 71, 647
Bso31I GGTCTC 1 cut(s) 985
Bsp1286I GDGCHC 3 cut(s) 89, 126, 1189
Bsp143I GATC 6 cut(s) 76, 140, 217, 367, 592, 1349
Bsp1720I GCTNAGC 1 cut(s) 478
Bsp19I CCATGG 1 cut(s) 300
BspACI CCGC 2 cut(s) 39, 924
BspANI GGCC 3 cut(s) 30, 71, 647
BspCNI CTCAG 5 cut(s) 10, 484, 491, 915, 1213
BspLI GGNNCC 1 cut(s) 369
BspMAI CTGCAG 1 cut(s) 451
BspMI ACCTGC 1 cut(s) 92
BspPI GGATC 6 cut(s) 71, 148, 362, 375, 600, 1357
BspTNI GGTCTC 1 cut(s) 985
BsrBI CCGCTC 1 cut(s) 924
BsrFI RCCGGY 1 cut(s) 452
BsrI ACTGG 3 cut(s) 439, 947, 1054
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 6 cut(s) 80, 300, 335, 356, 950, 1028
BssMI GATC 6 cut(s) 76, 140, 217, 367, 592, 1349
BssNAI GTATAC 2 cut(s) 1047, 1125
BssT1I CCWWGG 1 cut(s) 300
Bst1107I GTATAC 2 cut(s) 1047, 1125
Bst2UI CCWGG 7 cut(s) 81, 336, 358, 762, 951, 1029, 1075
Bst4CI ACNGT 4 cut(s) 8, 558, 1085, 1361
Bst6I CTCTTC 3 cut(s) 657, 809, 1083
BstBAI YACGTR 1 cut(s) 918
BstDEI CTNAG 8 cut(s) 18, 399, 478, 492, 902, 1130, 1143, 1221
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 2 cut(s) 392, 1317
BstKTI GATC 6 cut(s) 79, 143, 220, 370, 595, 1352
BstMAI GTCTC 6 cut(s) 542, 806, 905, 985, 1031, 1110
BstMBI GATC 6 cut(s) 76, 140, 217, 367, 592, 1349
BstMWI GCNNNNNNNGC 3 cut(s) 44, 921, 1211
BstNI CCWGG 7 cut(s) 81, 336, 358, 762, 951, 1029, 1075
BstNSI RCATGY 1 cut(s) 1209
BstPAI GACNNNNGTC 1 cut(s) 534
BstSCI CCNGG 8 cut(s) 79, 334, 356, 708, 760, 949, 1027, 1073
BstSFI CTRYAG 2 cut(s) 447, 601
BstSLI GKGCMC 1 cut(s) 1189
BstV1I GCAGC 4 cut(s) 461, 486, 640, 1214
BstV2I GAAGAC 3 cut(s) 851, 1118, 1233
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BstZ17I GTATAC 2 cut(s) 1047, 1125
BsuI GTATCC 1 cut(s) 939
BsuRI GGCC 3 cut(s) 30, 71, 647
BtgI CCRYGG 1 cut(s) 300
BtgZI GCGATG 2 cut(s) 28, 540
BtsCI GGATG 2 cut(s) 392, 1317
BtsI GCAGTG 2 cut(s) 750, 1263
BtsIMutI CAGTG 4 cut(s) 750, 810, 1090, 1263
BveI ACCTGC 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 3 cut(s) 70, 645, 755
Csp6I GTAC 5 cut(s) 507, 515, 772, 1081, 1227
CviAII CATG 6 cut(s) 183, 301, 471, 541, 776, 1206
CviQI GTAC 5 cut(s) 507, 515, 772, 1081, 1227
DdeI CTNAG 8 cut(s) 18, 399, 478, 492, 902, 1130, 1143, 1221
DpnI GATC 6 cut(s) 78, 142, 219, 369, 594, 1351
DpnII GATC 6 cut(s) 76, 140, 217, 367, 592, 1349
Eam1104I CTCTTC 3 cut(s) 657, 809, 1083
EarI CTCTTC 3 cut(s) 657, 809, 1083
Ecl136II GAGCTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 30
Eco24I GRGCYC 2 cut(s) 89, 126
Eco31I GGTCTC 1 cut(s) 985
Eco47I GGWCC 1 cut(s) 755
Eco53kI GAGCTC 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 1130
Eco72I CACGTG 1 cut(s) 918
EcoICRI GAGCTC 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 755
EcoRI GAATTC 1 cut(s) 654
EcoRII CCWGG 7 cut(s) 79, 334, 356, 760, 949, 1027, 1073
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 2 cut(s) 89, 126
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 6 cut(s) 186, 304, 474, 544, 779, 1209
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FatI CATG 6 cut(s) 182, 300, 470, 540, 775, 1205
FblI GTMKAC 4 cut(s) 439, 928, 1046, 1124
Fnu4HI GCNGC 6 cut(s) 39, 450, 475, 629, 1203, 1380
FokI GGATG 2 cut(s) 379, 1324
FriOI GRGCYC 2 cut(s) 89, 126
Fsp4HI GCNGC 6 cut(s) 39, 450, 475, 629, 1203, 1380
FspBI CTAG 3 cut(s) 93, 504, 1308
GluI GCNGC 6 cut(s) 39, 450, 475, 629, 1203, 1380
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 3 cut(s) 30, 71, 647
HapII CCGG 3 cut(s) 453, 709, 852
Hin1II CATG 6 cut(s) 186, 304, 474, 544, 779, 1209
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HindIII AAGCTT 1 cut(s) 345
HinfI GANTC 4 cut(s) 176, 305, 494, 956
HpaI GTTAAC 1 cut(s) 408
HpaII CCGG 3 cut(s) 453, 709, 852
HphI GGTGA 3 cut(s) 248, 819, 1330
Hpy166II GTNNAC 7 cut(s) 408, 440, 841, 929, 1047, 1125, 1187
Hpy188I TCNGA 8 cut(s) 19, 283, 418, 493, 592, 622, 1110, 1222
Hpy188III TCNNGA 4 cut(s) 130, 138, 221, 659
Hpy8I GTNNAC 7 cut(s) 408, 440, 841, 929, 1047, 1125, 1187
HpyAV CCTTC 6 cut(s) 41, 358, 381, 498, 676, 1052
HpyCH4III ACNGT 4 cut(s) 8, 558, 1085, 1361
HpyCH4IV ACGT 2 cut(s) 513, 917
HpyCH4V TGCA 9 cut(s) 422, 449, 474, 544, 1007, 1187, 1256, 1261, 1379
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 921, 1211
HpyF3I CTNAG 8 cut(s) 18, 399, 478, 492, 902, 1130, 1143, 1221
HpySE526I ACGT 2 cut(s) 513, 917
Hsp92II CATG 6 cut(s) 186, 304, 474, 544, 779, 1209
KspAI GTTAAC 1 cut(s) 408
Kzo9I GATC 6 cut(s) 76, 140, 217, 367, 592, 1349
LmnI GCTCC 2 cut(s) 84, 921
Lsp1109I GCAGC 4 cut(s) 461, 486, 640, 1214
LweI GCATC 2 cut(s) 409, 1243
MaeI CTAG 3 cut(s) 93, 504, 1308
MaeII ACGT 2 cut(s) 513, 917
MaeIII GTNAC 2 cut(s) 430, 1327
MalI GATC 6 cut(s) 78, 142, 219, 369, 594, 1351
MbiI CCGCTC 1 cut(s) 924
MboI GATC 6 cut(s) 76, 140, 217, 367, 592, 1349
MboII GAAGA 8 cut(s) 217, 224, 674, 796, 851, 1100, 1123, 1233
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 3 cut(s) 89, 126, 1189
MlyI GAGTC 2 cut(s) 170, 503
MseI TTAA 3 cut(s) 407, 561, 1293
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 3 cut(s) 453, 709, 852
MspR9I CCNGG 8 cut(s) 81, 336, 358, 710, 762, 951, 1029, 1075
Mva1269I GAATGC 2 cut(s) 160, 1009
MvaI CCWGG 7 cut(s) 81, 336, 358, 762, 951, 1029, 1075
MwoI GCNNNNNNNGC 3 cut(s) 44, 921, 1211
NciI CCSGG 1 cut(s) 710
NcoI CCATGG 1 cut(s) 300
NdeII GATC 6 cut(s) 76, 140, 217, 367, 592, 1349
NlaIII CATG 6 cut(s) 186, 304, 474, 544, 779, 1209
NlaIV GGNNCC 1 cut(s) 369
NspI RCATGY 1 cut(s) 1209
PceI AGGCCT 1 cut(s) 30
PctI GAATGC 2 cut(s) 160, 1009
PfeI GAWTC 2 cut(s) 305, 956
PflFI GACNNNGTC 1 cut(s) 1160
PflMI CCANNNNNTGG 1 cut(s) 642
PfoI TCCNGGA 1 cut(s) 1073
PkrI GCNGC 6 cut(s) 40, 451, 476, 630, 1204, 1381
PleI GAGTC 2 cut(s) 170, 502
PmaCI CACGTG 1 cut(s) 918
PmlI CACGTG 1 cut(s) 918
PpsI GAGTC 2 cut(s) 170, 502
Ppu21I YACGTR 1 cut(s) 918
PpuMI RGGWCCY 1 cut(s) 755
PshAI GACNNNNGTC 1 cut(s) 534
Psp124BI GAGCTC 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 755
Psp6I CCWGG 7 cut(s) 79, 334, 356, 760, 949, 1027, 1073
PspCI CACGTG 1 cut(s) 918
PspGI CCWGG 7 cut(s) 79, 334, 356, 760, 949, 1027, 1073
PspN4I GGNNCC 1 cut(s) 369
PspPI GGNCC 3 cut(s) 70, 645, 755
PspPPI RGGWCCY 1 cut(s) 755
PstI CTGCAG 1 cut(s) 451
PsuI RGATCY 1 cut(s) 367
PsyI GACNNNGTC 1 cut(s) 1160
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 5 cut(s) 508, 516, 773, 1082, 1228
RsaNI GTAC 5 cut(s) 507, 515, 772, 1081, 1227
SacI GAGCTC 1 cut(s) 89
SaqAI TTAA 3 cut(s) 407, 561, 1293
SatI GCNGC 6 cut(s) 39, 450, 475, 629, 1203, 1380
Sau3AI GATC 6 cut(s) 76, 140, 217, 367, 592, 1349
Sau96I GGNCC 3 cut(s) 70, 645, 755
SchI GAGTC 2 cut(s) 170, 503
ScrFI CCNGG 8 cut(s) 81, 336, 358, 710, 762, 951, 1029, 1075
SduI GDGCHC 3 cut(s) 89, 126, 1189
SfaNI GCATC 2 cut(s) 409, 1243
SfcI CTRYAG 2 cut(s) 447, 601
SinI GGWCC 1 cut(s) 755
SmlI CTYRAG 2 cut(s) 128, 1282
SmoI CTYRAG 2 cut(s) 128, 1282
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 2 cut(s) 39, 924
SspI AATATT 1 cut(s) 1195
SspMI CTAG 3 cut(s) 93, 504, 1308
SstI GAGCTC 1 cut(s) 89
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 8 cut(s) 79, 334, 356, 708, 760, 949, 1027, 1073
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 4 cut(s) 8, 558, 1085, 1361
TaiI ACGT 2 cut(s) 516, 920
TaqI TCGA 2 cut(s) 579, 1372
TatI WGTACW 1 cut(s) 1080
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 2 cut(s) 305, 956
Tru1I TTAA 3 cut(s) 407, 561, 1293
Tru9I TTAA 3 cut(s) 407, 561, 1293
TscAI CASTG 4 cut(s) 750, 817, 1090, 1263
TseI GCWGC 5 cut(s) 449, 474, 628, 1202, 1379
TspDTI ATGAA 5 cut(s) 213, 218, 459, 732, 1331
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 4 cut(s) 750, 817, 1090, 1263
Tth111I GACNNNGTC 1 cut(s) 1160
Van91I CCANNNNNTGG 1 cut(s) 642
VneI GTGCAC 1 cut(s) 1185
VpaK11BI GGWCC 1 cut(s) 755
XapI RAATTY 5 cut(s) 548, 654, 672, 874, 1289
XceI RCATGY 1 cut(s) 1209
XcmI CCANNNNNNNNNTGG 1 cut(s) 639
XmiI GTMKAC 4 cut(s) 439, 928, 1046, 1124
XspI CTAG 3 cut(s) 93, 504, 1308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.