MD09G1247000.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006585
Physical Location & Seq
Forward (+)
31673970 .. 31678390
4421 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1247000.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGAGCATGGACGATGAAGACAACGACGACGACGATGATGATAATGACTTTGATTGTGATGACAACGATGGTTTTCTGTATGATGACGGTGATGATTGCATGACTCTGCAATCTCAGTTTGATAATGTGGACTTGCCTCCTGGTGTCGAAGCAACTCTTCCCTGGTTGAATGATCCTTCACCGAGTGAGAAGCCATCCACTAAGACGCTAGCTATTTCAGATCTACCACCATGTAGTTCAACTGTTCCTTCTGAGGCAAGTTCTAGTAAGGAACCGGCAGACAGCAATGATGACAGTATCATGCAAAAGTCTCAACAGTTTAAGAAATTTGATATGGTGGAAGATTTCTCGGATCACCACTTTAGCCGCATGGGATTTTCAGATGAGCAGCCACCCAGATATTGGGCAAAGCGAATTCAGGATGAATGGAAGATTTTGGAGAAGGACTTACCAGACACTATATTTGTCAGGGTTTATGAAGCAAGAATGGAACTTCTGAGGGGTGTCATTATAGGACCTCCGGGCACTCCTTACCATGACGGTCTTTTTGTCTTTGATTGTCTCTTCCCAACTAACTATCCTAAATCACCACCGATGGTGTACTACTATTCAGGTGGTCTTCGATTGAATCCAAATTTGTACGAGTGTGGGAAAGTTTGTCTGAGTCTCCTAGGCACTTGGAGTGGCAAACAAAATGAAAATTGGGTCCCAGGGCAATCAACAATGCTACAAGTTTTAGTCTCTATACAAGCTCTTATTTTGAATGCAGAGCCCTTCTTCAATGAACCTGGGCATGAGTCATCGTATGTTGGAGCAGAAGGGCAAAGGCAATCCAGAAAGTACAATGAGGATACCTTTATTCTATCTCTAAAGACGATGATTTATACACTAAGGAGGCCGCCGAAGTATTTTGAGGACTTTGTCTTGGGTCATTTTCGCGAACGTGCACACAATATTCTGGCAGCATGTAAAGCATATAGTGAGGGCACTCTGGTGGGGTCTGATATCACATCCCAGAATGCAGAAAAAGGTGGCTCTGCACAATTCAAGGCAAGTGTAGTTAAGATGATGAATACACTTATTACACATTTCACCAGAAATGGATCAACCGACTGTGAGAAGTTTAGAGTTGAAGCCTGTATCACATTGGACTAG

Protein Analysis

385

Amino Acids

43.37

Weight (kDa)

4.53

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 138 - 267 1.2e-21 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 402
AccII CGCG 1 cut(s) 939
AciI CCGC 2 cut(s) 367, 899
AclWI GGATC 3 cut(s) 167, 360, 1111
AcsI RAATTY 3 cut(s) 326, 414, 634
AdeI CACNNNGTG 1 cut(s) 185
AfaI GTAC 3 cut(s) 602, 641, 842
AfiI CCNNNNNNNGG 1 cut(s) 402
AgsI TTSAA 7 cut(s) 169, 240, 628, 763, 781, 1048, 1133
AjnI CCWGG 4 cut(s) 139, 161, 709, 787
AleI CACNNNNGTG 1 cut(s) 992
AluBI AGCT 2 cut(s) 212, 752
AluI AGCT 2 cut(s) 212, 752
Alw21I GWGCWC 1 cut(s) 949
Alw26I GTCTC 4 cut(s) 315, 566, 671, 745
Alw44I GTGCAC 1 cut(s) 945
AlwI GGATC 3 cut(s) 167, 360, 1111
AoxI GGCC 1 cut(s) 896
ApaLI GTGCAC 1 cut(s) 945
ApeKI GCWGC 2 cut(s) 388, 962
ApoI RAATTY 3 cut(s) 326, 414, 634
ArsI GACNNNNNNTTYG 4 cut(s) 446, 478, 530, 562
AspA2I CCTAGG 1 cut(s) 670
AspS9I GGNCC 2 cut(s) 515, 706
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 5 cut(s) 101, 171, 347, 579, 1084
AsuNHI GCTAGC 1 cut(s) 208
AvaII GGWCC 2 cut(s) 515, 706
AvrII CCTAGG 1 cut(s) 670
BaeGI GKGCMC 3 cut(s) 527, 949, 989
BanII GRGCYC 1 cut(s) 774
BbsI GAAGAC 2 cut(s) 24, 611
Bbv12I GWGCWC 1 cut(s) 949
BbvI GCAGC 2 cut(s) 400, 974
BccI CCATC 3 cut(s) 62, 202, 589
BciT130I CCWGG 4 cut(s) 141, 163, 711, 789
BciVI GTATCC 1 cut(s) 844
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 4 cut(s) 315, 566, 671, 745
BfaI CTAG 4 cut(s) 209, 264, 671, 1153
BfuI GTATCC 1 cut(s) 844
BglII AGATCT 1 cut(s) 220
BisI GCNGC 4 cut(s) 367, 389, 899, 963
BlnI CCTAGG 1 cut(s) 670
BlsI GCNGC 4 cut(s) 368, 390, 900, 964
Bme1390I CCNGG 5 cut(s) 141, 163, 522, 711, 789
Bme18I GGWCC 2 cut(s) 515, 706
BmgT120I GGNCC 2 cut(s) 515, 706
BmiI GGNNCC 3 cut(s) 273, 707, 708
BmrFI CCNGG 5 cut(s) 141, 163, 522, 711, 789
BmtI GCTAGC 1 cut(s) 212
BpiI GAAGAC 2 cut(s) 24, 611
BpuMI CCSGG 1 cut(s) 522
BsaJI CCNNGG 5 cut(s) 161, 670, 709, 710, 788
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse118I RCCGGY 1 cut(s) 274
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 4 cut(s) 141, 163, 711, 789
BseDI CCNNGG 5 cut(s) 161, 670, 709, 710, 788
BseGI GGATG 3 cut(s) 194, 427, 1010
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 4 cut(s) 128, 243, 488, 653
BseSI GKGCMC 3 cut(s) 527, 949, 989
BseXI GCAGC 2 cut(s) 400, 974
BsgI GTGCAG 1 cut(s) 1023
Bsh1236I CGCG 1 cut(s) 939
BshFI GGCC 1 cut(s) 898
BsiHKAI GWGCWC 1 cut(s) 949
BsiSI CCGG 2 cut(s) 275, 521
BslFI GGGAC 1 cut(s) 692
BslI CCNNNNNNNGG 1 cut(s) 402
BsmAI GTCTC 4 cut(s) 315, 566, 671, 745
BsmFI GGGAC 1 cut(s) 692
BsmI GAATGC 2 cut(s) 769, 1024
BsnI GGCC 1 cut(s) 898
Bsp1286I GDGCHC 4 cut(s) 527, 774, 949, 989
Bsp143I GATC 4 cut(s) 172, 220, 352, 1103
Bsp68I TCGCGA 1 cut(s) 939
BspACI CCGC 2 cut(s) 367, 899
BspANI GGCC 1 cut(s) 898
BspCNI CTCAG 4 cut(s) 127, 244, 489, 654
BspFNI CGCG 1 cut(s) 939
BspLI GGNNCC 3 cut(s) 273, 707, 708
BspOI GCTAGC 1 cut(s) 212
BspPI GGATC 3 cut(s) 167, 360, 1111
BsrDI GCAATG 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 274
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 5 cut(s) 161, 670, 709, 710, 788
BssMI GATC 4 cut(s) 172, 220, 352, 1103
BssT1I CCWWGG 1 cut(s) 670
Bst2UI CCWGG 4 cut(s) 141, 163, 711, 789
Bst4CI ACNGT 6 cut(s) 89, 244, 296, 318, 542, 1115
Bst6I CTCTTC 2 cut(s) 162, 569
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 6 cut(s) 114, 201, 252, 497, 662, 890
BstF5I GGATG 3 cut(s) 194, 427, 1010
BstFNI CGCG 1 cut(s) 939
BstKTI GATC 4 cut(s) 175, 223, 355, 1106
BstMAI GTCTC 4 cut(s) 315, 566, 671, 745
BstMBI GATC 4 cut(s) 172, 220, 352, 1103
BstMWI GCNNNNNNNGC 1 cut(s) 971
BstNI CCWGG 4 cut(s) 141, 163, 711, 789
BstNSI RCATGY 1 cut(s) 969
BstSCI CCNGG 5 cut(s) 139, 161, 520, 709, 787
BstSLI GKGCMC 3 cut(s) 527, 949, 989
BstUI CGCG 1 cut(s) 939
BstV1I GCAGC 2 cut(s) 400, 974
BstV2I GAAGAC 2 cut(s) 24, 611
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BsuI GTATCC 1 cut(s) 844
BsuRI GGCC 1 cut(s) 898
BtsCI GGATG 3 cut(s) 194, 427, 1010
BtuMI TCGCGA 1 cut(s) 939
Cac8I GCNNGC 1 cut(s) 210
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 515, 706
CseI GACGC 1 cut(s) 214
Csp6I GTAC 3 cut(s) 601, 640, 841
CviAII CATG 8 cut(s) 7, 100, 231, 301, 370, 536, 794, 966
CviJI RGCY 9 cut(s) 193, 212, 366, 391, 752, 772, 898, 1035, 1136
CviKI_1 RGCY 9 cut(s) 193, 212, 366, 391, 752, 772, 898, 1035, 1136
CviQI GTAC 3 cut(s) 601, 640, 841
DdeI CTNAG 6 cut(s) 114, 201, 252, 497, 662, 890
DpnI GATC 4 cut(s) 174, 222, 354, 1105
DpnII GATC 4 cut(s) 172, 220, 352, 1103
DraIII CACNNNGTG 1 cut(s) 185
Eam1104I CTCTTC 2 cut(s) 162, 569
EarI CTCTTC 2 cut(s) 162, 569
Eco130I CCWWGG 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 774
Eco32I GATATC 1 cut(s) 1006
Eco47I GGWCC 2 cut(s) 515, 706
EcoO109I RGGNCCY 2 cut(s) 515, 706
EcoRI GAATTC 1 cut(s) 414
EcoRII CCWGG 4 cut(s) 139, 161, 709, 787
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 774
ErhI CCWWGG 1 cut(s) 670
FaeI CATG 8 cut(s) 10, 103, 234, 304, 373, 539, 797, 969
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FaqI GGGAC 1 cut(s) 692
FatI CATG 8 cut(s) 6, 99, 230, 300, 369, 535, 793, 965
Fnu4HI GCNGC 4 cut(s) 367, 389, 899, 963
FokI GGATG 3 cut(s) 181, 434, 997
FriOI GRGCYC 1 cut(s) 774
Fsp4HI GCNGC 4 cut(s) 367, 389, 899, 963
FspBI CTAG 4 cut(s) 209, 264, 671, 1153
GluI GCNGC 4 cut(s) 367, 389, 899, 963
HaeIII GGCC 1 cut(s) 898
HapII CCGG 2 cut(s) 275, 521
HgaI GACGC 1 cut(s) 214
Hin1II CATG 8 cut(s) 10, 103, 234, 304, 373, 539, 797, 969
HinfI GANTC 4 cut(s) 103, 628, 664, 797
HpaII CCGG 2 cut(s) 275, 521
HphI GGTGA 5 cut(s) 101, 171, 347, 579, 1084
Hpy166II GTNNAC 3 cut(s) 130, 601, 947
Hpy188I TCNGA 7 cut(s) 220, 253, 352, 382, 498, 663, 1003
Hpy188III TCNNGA 3 cut(s) 419, 834, 938
Hpy8I GTNNAC 3 cut(s) 130, 601, 947
Hpy99I CGWCG 3 cut(s) 29, 32, 35
HpyAV CCTTC 5 cut(s) 186, 258, 436, 784, 812
HpyCH4III ACNGT 6 cut(s) 89, 244, 296, 318, 542, 1115
HpyCH4IV ACGT 1 cut(s) 943
HpyCH4V TGCA 7 cut(s) 99, 109, 304, 767, 947, 1022, 1040
HpyF10VI GCNNNNNNNGC 1 cut(s) 971
HpyF3I CTNAG 6 cut(s) 114, 201, 252, 497, 662, 890
HpySE526I ACGT 1 cut(s) 943
Hsp92II CATG 8 cut(s) 10, 103, 234, 304, 373, 539, 797, 969
KflI GGGWCCC 1 cut(s) 706
Kzo9I GATC 4 cut(s) 172, 220, 352, 1103
LmnI GCTCC 1 cut(s) 812
Lsp1109I GCAGC 2 cut(s) 400, 974
MaeI CTAG 4 cut(s) 209, 264, 671, 1153
MaeII ACGT 1 cut(s) 943
MalI GATC 4 cut(s) 174, 222, 354, 1105
MboI GATC 4 cut(s) 172, 220, 352, 1103
MboII GAAGA 7 cut(s) 29, 149, 353, 442, 556, 611, 769
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 4 cut(s) 527, 774, 949, 989
MluCI AATT 5 cut(s) 326, 414, 634, 700, 1043
MlyI GAGTC 3 cut(s) 97, 673, 806
MmeI TCCRAC 1 cut(s) 790
MnlI CCTC 8 cut(s) 147, 247, 492, 528, 841, 888, 907, 976
MseI TTAA 2 cut(s) 321, 1062
MslI CAYNNNNRTG 1 cut(s) 992
MspI CCGG 2 cut(s) 275, 521
MspR9I CCNGG 5 cut(s) 141, 163, 522, 711, 789
Mva1269I GAATGC 2 cut(s) 769, 1024
MvaI CCWGG 4 cut(s) 141, 163, 711, 789
MvnI CGCG 1 cut(s) 939
MwoI GCNNNNNNNGC 1 cut(s) 971
NciI CCSGG 1 cut(s) 522
NdeII GATC 4 cut(s) 172, 220, 352, 1103
NheI GCTAGC 1 cut(s) 208
NlaIII CATG 8 cut(s) 10, 103, 234, 304, 373, 539, 797, 969
NlaIV GGNNCC 3 cut(s) 273, 707, 708
NruI TCGCGA 1 cut(s) 939
NspI RCATGY 1 cut(s) 969
OliI CACNNNNGTG 1 cut(s) 992
PasI CCCWGGG 1 cut(s) 710
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 2 cut(s) 769, 1024
PfeI GAWTC 1 cut(s) 628
PflFI GACNNNGTC 1 cut(s) 920
PflMI CCANNNNNTGG 1 cut(s) 402
PkrI GCNGC 4 cut(s) 368, 390, 900, 964
PleI GAGTC 3 cut(s) 97, 672, 805
PpsI GAGTC 3 cut(s) 97, 672, 805
PpuMI RGGWCCY 2 cut(s) 515, 706
Psp5II RGGWCCY 2 cut(s) 515, 706
Psp6I CCWGG 4 cut(s) 139, 161, 709, 787
PspGI CCWGG 4 cut(s) 139, 161, 709, 787
PspN4I GGNNCC 3 cut(s) 273, 707, 708
PspPI GGNCC 2 cut(s) 515, 706
PspPPI RGGWCCY 2 cut(s) 515, 706
PsuI RGATCY 1 cut(s) 220
PsyI GACNNNGTC 1 cut(s) 920
RruI TCGCGA 1 cut(s) 939
RsaI GTAC 3 cut(s) 602, 641, 842
RsaNI GTAC 3 cut(s) 601, 640, 841
RseI CAYNNNNRTG 1 cut(s) 992
SaqAI TTAA 2 cut(s) 321, 1062
SatI GCNGC 4 cut(s) 367, 389, 899, 963
Sau3AI GATC 4 cut(s) 172, 220, 352, 1103
Sau96I GGNCC 2 cut(s) 515, 706
SchI GAGTC 3 cut(s) 97, 673, 806
ScrFI CCNGG 5 cut(s) 141, 163, 522, 711, 789
SduI GDGCHC 4 cut(s) 527, 774, 949, 989
SetI ASST 8 cut(s) 214, 520, 616, 754, 790, 857, 946, 1033
SinI GGWCC 2 cut(s) 515, 706
SmiMI CAYNNNNRTG 1 cut(s) 992
Sse9I AATT 5 cut(s) 326, 414, 634, 700, 1043
SsiI CCGC 2 cut(s) 367, 899
SspI AATATT 1 cut(s) 955
SspMI CTAG 4 cut(s) 209, 264, 671, 1153
StyD4I CCNGG 5 cut(s) 139, 161, 520, 709, 787
StyI CCWWGG 1 cut(s) 670
TaaI ACNGT 6 cut(s) 89, 244, 296, 318, 542, 1115
TaiI ACGT 1 cut(s) 946
TaqI TCGA 2 cut(s) 147, 622
TasI AATT 5 cut(s) 326, 414, 634, 700, 1043
TatI WGTACW 2 cut(s) 600, 840
TauI GCSGC 2 cut(s) 369, 901
TfiI GAWTC 1 cut(s) 628
Tru1I TTAA 2 cut(s) 321, 1062
Tru9I TTAA 2 cut(s) 321, 1062
TseI GCWGC 2 cut(s) 388, 962
TspDTI ATGAA 6 cut(s) 30, 438, 492, 711, 798, 1085
Tth111I GACNNNGTC 1 cut(s) 920
Van91I CCANNNNNTGG 1 cut(s) 402
VneI GTGCAC 1 cut(s) 945
VpaK11BI GGWCC 2 cut(s) 515, 706
XapI RAATTY 3 cut(s) 326, 414, 634
XceI RCATGY 1 cut(s) 969
XcmI CCANNNNNNNNNTGG 1 cut(s) 399
XmaJI CCTAGG 1 cut(s) 670
XspI CTAG 4 cut(s) 209, 264, 671, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.