FvH4_6g26220

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006585
Physical Location & Seq
Reverse (-)
20071503 .. 20076235
4733 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26220.t2

Sequence Viewer

Length: 1221 bp
ATGGACTCTGAAGCCACCATCTCCAAGAAACTCAAACAAAATCAGGCCTGTGACCCTGGGAGCTCTGCTAGTTTAGCAGGCTCTTTAGATAGTTTTGAGCCCCTCAAGAGTTCAGGATCAACCAATTCGATTGCTCTCAAAAGTTCCAATGAGTCGTCAGATTTGCCTCCTGGGGTGGAAGCCTCACTTCCCTGGTTGAAGGATCCTTCTCCAAGTGAAAACACCCCATCCACTAAGAAGTTAACTATTTCTGATGCACCATTTGTAACTGGATCTGCATCTGGTTCAATGAGTACATCTGCTGCATGTAGTTCAGCTGGTCCTTCTGAGTCAAGTTCTAGTAAGAACGTACAACACAGCAATGATGATAGTCTCACCCAAAAATTTCAACAATTTAAGCAATTTGATGTTGTCGAGGATTTCTCGGATCATCACTATAGTCGCATGGGATTCTCAGATGAGCAGCCACCCAAGAATTGGGCAAAGAGAATTCAGGAAGAGTGGAAAATTTTGGAGAATGACTTGCCTGACACTATATTTGTCCGGGTTTATGAAGCAAGAATGGAGCTTCTACGGGCGGTGATTATAGGACCAACAGGCACTCCATACCATGATGGTCTTTTTGTCTTTGATTGTCTCTTCCCCACTGACTATCCTAATTCACCACCTATGGTGTACTACTATTCTGGTGGTCTTCGGCTAAATCCAAATTTATACGAATGTGGGAAAGTTTGTCTTAGTCTTTTAGGCACGTGGAGCGGTAAGCAGAGAGAGAACTGGATACCTGGGGAATCAACAATGCTACAAGTTTTGGTCTCTATACAAGCTCTTATTCTGAATGCAGACCCCTATTTCAATGAGCCTGGGTATGAGAAAACGTCTACTACAGTAGAAGGGCAAAAGCAATCCAGGAAGTACAGTGAGGAGGCCTTTATTATGTCTCTGAAGACAATGATGTATACACTAAGGAAGCCACCTAAGTATTTTGAGGACTTTGTCTCGGGACATTTTCGCAATCGTGCACATAATATTCTGGAAGCATGTAAGGCATATTCTGAGGGTACAGTGGTAGGCTCTGCTATTGGAGATGCAGTGCAGAATGGAGTTGACAGTAGCTCAAGAGAATTTAAGTCATCTGTGGCTAGGATGATGAATACACTTGTTACACATTTCACCAGAAATGGATCAACAGACTGTGAGCAGTTTCGAGGTGCAGCTTAA

Protein Analysis

407

Amino Acids

44.73

Weight (kDa)

5.41

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 207 - 342 7.4e-22 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 477
AccBSI CCGCTC 1 cut(s) 759
AccI GTMKAC 2 cut(s) 881, 959
AciI CCGC 2 cut(s) 578, 759
AclWI GGATC 6 cut(s) 124, 197, 210, 280, 435, 1192
AcsI RAATTY 5 cut(s) 383, 489, 507, 709, 1124
AcuI CTGAAG 2 cut(s) 30, 965
AcvI CACGTG 1 cut(s) 753
AfaI GTAC 5 cut(s) 295, 351, 677, 917, 1063
AfiI CCNNNNNNNGG 1 cut(s) 477
AgsI TTSAA 4 cut(s) 199, 288, 389, 856
AjnI CCWGG 6 cut(s) 55, 169, 191, 784, 862, 908
AluBI AGCT 6 cut(s) 63, 317, 568, 827, 1116, 1217
AluI AGCT 6 cut(s) 63, 317, 568, 827, 1116, 1217
Alw21I GWGCWC 2 cut(s) 65, 1024
Alw26I GTCTC 5 cut(s) 377, 641, 820, 945, 1003
Alw44I GTGCAC 1 cut(s) 1020
AlwI GGATC 6 cut(s) 124, 197, 210, 280, 435, 1192
Ama87I CYCGRG 1 cut(s) 1000
AoxI GGCC 2 cut(s) 45, 927
ApaLI GTGCAC 1 cut(s) 1020
ApeKI GCWGC 3 cut(s) 302, 463, 1214
ApoI RAATTY 5 cut(s) 383, 489, 507, 709, 1124
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
AspS9I GGNCC 2 cut(s) 320, 590
AsuC2I CCSGG 1 cut(s) 545
AsuHPI GGTGA 4 cut(s) 367, 592, 654, 1165
AvaI CYCGRG 1 cut(s) 1000
AvaII GGWCC 2 cut(s) 320, 590
BaeGI GKGCMC 1 cut(s) 1024
BaeI ACNNNNGTAYC 2 cut(s) 1053, 1086
BamHI GGATCC 1 cut(s) 202
BanII GRGCYC 2 cut(s) 65, 102
BbrPI CACGTG 1 cut(s) 753
BbsI GAAGAC 2 cut(s) 686, 953
Bbv12I GWGCWC 2 cut(s) 65, 1024
BbvI GCAGC 2 cut(s) 289, 475
BccI CCATC 3 cut(s) 26, 235, 608
BciT130I CCWGG 6 cut(s) 57, 171, 193, 786, 864, 910
BciVI GTATCC 1 cut(s) 774
BcnI CCSGG 1 cut(s) 545
BcoDI GTCTC 5 cut(s) 377, 641, 820, 945, 1003
BfaI CTAG 3 cut(s) 69, 339, 1143
BfmI CTRYAG 2 cut(s) 436, 885
BfuI GTATCC 1 cut(s) 774
BisI GCNGC 3 cut(s) 303, 464, 1215
BlsI GCNGC 3 cut(s) 304, 465, 1216
Bme1390I CCNGG 7 cut(s) 57, 171, 193, 545, 786, 864, 910
Bme18I GGWCC 2 cut(s) 320, 590
BmeT110I CYCGRG 1 cut(s) 1000
BmgT120I GGNCC 2 cut(s) 320, 590
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 7 cut(s) 57, 171, 193, 545, 786, 864, 910
BmsI GCATC 3 cut(s) 244, 287, 1078
BpiI GAAGAC 2 cut(s) 686, 953
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpuEI CTTGAG 2 cut(s) 89, 1102
BpuMI CCSGG 1 cut(s) 545
BsaAI YACGTR 1 cut(s) 753
BsaBI GATNNNNATC 1 cut(s) 277
BsaI GGTCTC 1 cut(s) 820
BsaJI CCNNGG 6 cut(s) 55, 56, 170, 191, 785, 863
BsaXI ACNNNNNCTCC 4 cut(s) 586, 616, 1077, 1107
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse1I ACTGG 2 cut(s) 274, 782
Bse3DI GCAATG 1 cut(s) 367
Bse8I GATNNNNATC 1 cut(s) 277
BseBI CCWGG 6 cut(s) 57, 171, 193, 786, 864, 910
BseDI CCNNGG 6 cut(s) 55, 56, 170, 191, 785, 863
BseGI GGATG 2 cut(s) 227, 1152
BseJI GATNNNNATC 1 cut(s) 277
BseLI CCNNNNNNNGG 1 cut(s) 477
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 3 cut(s) 318, 468, 1047
BseNI ACTGG 2 cut(s) 274, 782
BseRI GAGGAG 1 cut(s) 938
BseSI GKGCMC 1 cut(s) 1024
BseXI GCAGC 2 cut(s) 289, 475
BsgI GTGCAG 1 cut(s) 1115
BshFI GGCC 2 cut(s) 47, 929
BsiHKAI GWGCWC 2 cut(s) 65, 1024
BsiHKCI CYCGRG 1 cut(s) 1000
BsiSI CCGG 1 cut(s) 544
BslFI GGGAC 1 cut(s) 1017
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 5 cut(s) 377, 641, 820, 945, 1003
BsmFI GGGAC 1 cut(s) 1017
BsmI GAATGC 1 cut(s) 844
BsnI GGCC 2 cut(s) 47, 929
Bso31I GGTCTC 1 cut(s) 820
BsoBI CYCGRG 1 cut(s) 1000
Bsp1286I GDGCHC 3 cut(s) 65, 102, 1024
Bsp143I GATC 5 cut(s) 116, 202, 272, 427, 1184
BspACI CCGC 2 cut(s) 578, 759
BspANI GGCC 2 cut(s) 47, 929
BspCNI CTCAG 3 cut(s) 319, 467, 1048
BspLI GGNNCC 1 cut(s) 204
BspPI GGATC 6 cut(s) 124, 197, 210, 280, 435, 1192
BspTNI GGTCTC 1 cut(s) 820
BsrBI CCGCTC 1 cut(s) 759
BsrDI GCAATG 1 cut(s) 367
BsrI ACTGG 2 cut(s) 274, 782
BssECI CCNNGG 6 cut(s) 55, 56, 170, 191, 785, 863
BssMI GATC 5 cut(s) 116, 202, 272, 427, 1184
BssNAI GTATAC 1 cut(s) 960
Bst1107I GTATAC 1 cut(s) 960
Bst2UI CCWGG 6 cut(s) 57, 171, 193, 786, 864, 910
Bst4CI ACNGT 5 cut(s) 889, 920, 1066, 1112, 1196
Bst6I CTCTTC 2 cut(s) 492, 644
BstBAI YACGTR 1 cut(s) 753
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 7 cut(s) 234, 327, 454, 737, 965, 978, 1056
BstF5I GGATG 2 cut(s) 227, 1152
BstKTI GATC 5 cut(s) 119, 205, 275, 430, 1187
BstMAI GTCTC 5 cut(s) 377, 641, 820, 945, 1003
BstMBI GATC 5 cut(s) 116, 202, 272, 427, 1184
BstMWI GCNNNNNNNGC 3 cut(s) 74, 756, 1046
BstNI CCWGG 6 cut(s) 57, 171, 193, 786, 864, 910
BstNSI RCATGY 2 cut(s) 309, 1044
BstSCI CCNGG 7 cut(s) 55, 169, 191, 543, 784, 862, 908
BstSFI CTRYAG 2 cut(s) 436, 885
BstSLI GKGCMC 1 cut(s) 1024
BstV1I GCAGC 2 cut(s) 289, 475
BstV2I GAAGAC 2 cut(s) 686, 953
BstX2I RGATCY 2 cut(s) 202, 272
BstYI RGATCY 2 cut(s) 202, 272
BstZ17I GTATAC 1 cut(s) 960
BsuI GTATCC 1 cut(s) 774
BsuRI GGCC 2 cut(s) 47, 929
BtsCI GGATG 2 cut(s) 227, 1152
BtsI GCAGTG 1 cut(s) 1098
BtsIMutI CAGTG 4 cut(s) 645, 925, 1071, 1098
Cac8I GCNNGC 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 320, 590
Csp6I GTAC 5 cut(s) 294, 350, 676, 916, 1062
CviAII CATG 4 cut(s) 306, 445, 611, 1041
CviQI GTAC 5 cut(s) 294, 350, 676, 916, 1062
DdeI CTNAG 7 cut(s) 234, 327, 454, 737, 965, 978, 1056
DpnI GATC 5 cut(s) 118, 204, 274, 429, 1186
DpnII GATC 5 cut(s) 116, 202, 272, 427, 1184
Eam1104I CTCTTC 2 cut(s) 492, 644
EarI CTCTTC 2 cut(s) 492, 644
Ecl136II GAGCTC 1 cut(s) 63
Eco147I AGGCCT 2 cut(s) 47, 929
Eco24I GRGCYC 2 cut(s) 65, 102
Eco31I GGTCTC 1 cut(s) 820
Eco47I GGWCC 2 cut(s) 320, 590
Eco53kI GAGCTC 1 cut(s) 63
Eco57I CTGAAG 2 cut(s) 30, 965
Eco72I CACGTG 1 cut(s) 753
Eco88I CYCGRG 1 cut(s) 1000
EcoICRI GAGCTC 1 cut(s) 63
EcoRI GAATTC 1 cut(s) 489
EcoRII CCWGG 6 cut(s) 55, 169, 191, 784, 862, 908
EcoT38I GRGCYC 2 cut(s) 65, 102
FaeI CATG 4 cut(s) 309, 448, 614, 1044
FalI AAGNNNNNCTT 4 cut(s) 171, 203, 720, 752
FaqI GGGAC 1 cut(s) 1017
FatI CATG 4 cut(s) 305, 444, 610, 1040
FblI GTMKAC 2 cut(s) 881, 959
Fnu4HI GCNGC 3 cut(s) 303, 464, 1215
FokI GGATG 2 cut(s) 214, 1159
FriOI GRGCYC 2 cut(s) 65, 102
Fsp4HI GCNGC 3 cut(s) 303, 464, 1215
FspBI CTAG 3 cut(s) 69, 339, 1143
GluI GCNGC 3 cut(s) 303, 464, 1215
HaeIII GGCC 2 cut(s) 47, 929
HapII CCGG 1 cut(s) 544
Hin1II CATG 4 cut(s) 309, 448, 614, 1044
HincII GTYRAC 2 cut(s) 243, 1108
HindII GTYRAC 2 cut(s) 243, 1108
HinfI GANTC 5 cut(s) 5, 152, 329, 450, 791
HpaI GTTAAC 1 cut(s) 243
HpaII CCGG 1 cut(s) 544
HphI GGTGA 4 cut(s) 367, 592, 654, 1165
Hpy166II GTNNAC 6 cut(s) 243, 676, 882, 960, 1022, 1108
Hpy188I TCNGA 9 cut(s) 10, 160, 253, 328, 427, 457, 837, 945, 1057
Hpy188III TCNNGA 6 cut(s) 106, 114, 494, 1002, 1034, 1119
Hpy8I GTNNAC 6 cut(s) 243, 676, 882, 960, 1022, 1108
HpyAV CCTTC 4 cut(s) 193, 216, 333, 887
HpyCH4III ACNGT 5 cut(s) 889, 920, 1066, 1112, 1196
HpyCH4IV ACGT 3 cut(s) 348, 752, 878
HpyCH4V TGCA 8 cut(s) 257, 278, 305, 842, 1022, 1091, 1096, 1214
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 756, 1046
HpyF3I CTNAG 7 cut(s) 234, 327, 454, 737, 965, 978, 1056
HpySE526I ACGT 3 cut(s) 348, 752, 878
Hsp92II CATG 4 cut(s) 309, 448, 614, 1044
KspAI GTTAAC 1 cut(s) 243
Kzo9I GATC 5 cut(s) 116, 202, 272, 427, 1184
LmnI GCTCC 3 cut(s) 60, 565, 756
Lsp1109I GCAGC 2 cut(s) 289, 475
LweI GCATC 3 cut(s) 244, 287, 1078
MaeI CTAG 3 cut(s) 69, 339, 1143
MaeII ACGT 3 cut(s) 348, 752, 878
MaeIII GTNAC 3 cut(s) 50, 265, 1162
MalI GATC 5 cut(s) 118, 204, 274, 429, 1186
MbiI CCGCTC 1 cut(s) 759
MboI GATC 5 cut(s) 116, 202, 272, 427, 1184
MboII GAAGA 4 cut(s) 509, 631, 686, 958
MflI RGATCY 2 cut(s) 202, 272
MhlI GDGCHC 3 cut(s) 65, 102, 1024
MlyI GAGTC 2 cut(s) 161, 338
MnlI CCTC 9 cut(s) 113, 177, 193, 409, 916, 919, 982, 1051, 1202
MseI TTAA 4 cut(s) 242, 396, 1128, 1219
MslI CAYNNNNRTG 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 1 cut(s) 544
MspR9I CCNGG 7 cut(s) 57, 171, 193, 545, 786, 864, 910
Mva1269I GAATGC 1 cut(s) 844
MvaI CCWGG 6 cut(s) 57, 171, 193, 786, 864, 910
MwoI GCNNNNNNNGC 3 cut(s) 74, 756, 1046
NciI CCSGG 1 cut(s) 545
NdeII GATC 5 cut(s) 116, 202, 272, 427, 1184
NlaIII CATG 4 cut(s) 309, 448, 614, 1044
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 50
NspI RCATGY 2 cut(s) 309, 1044
PasI CCCWGGG 1 cut(s) 56
PceI AGGCCT 2 cut(s) 47, 929
PctI GAATGC 1 cut(s) 844
PfeI GAWTC 2 cut(s) 450, 791
PflFI GACNNNGTC 1 cut(s) 995
PflMI CCANNNNNTGG 1 cut(s) 477
PfoI TCCNGGA 1 cut(s) 908
PkrI GCNGC 3 cut(s) 304, 465, 1216
PleI GAGTC 2 cut(s) 160, 337
PmaCI CACGTG 1 cut(s) 753
PmlI CACGTG 1 cut(s) 753
PpsI GAGTC 2 cut(s) 160, 337
Ppu21I YACGTR 1 cut(s) 753
Psp124BI GAGCTC 1 cut(s) 65
Psp6I CCWGG 6 cut(s) 55, 169, 191, 784, 862, 908
PspCI CACGTG 1 cut(s) 753
PspGI CCWGG 6 cut(s) 55, 169, 191, 784, 862, 908
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 2 cut(s) 320, 590
PsuI RGATCY 2 cut(s) 202, 272
PsyI GACNNNGTC 1 cut(s) 995
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 5 cut(s) 295, 351, 677, 917, 1063
RsaNI GTAC 5 cut(s) 294, 350, 676, 916, 1062
RseI CAYNNNNRTG 1 cut(s) 360
SacI GAGCTC 1 cut(s) 65
SaqAI TTAA 4 cut(s) 242, 396, 1128, 1219
SatI GCNGC 3 cut(s) 303, 464, 1215
Sau3AI GATC 5 cut(s) 116, 202, 272, 427, 1184
Sau96I GGNCC 2 cut(s) 320, 590
SchI GAGTC 2 cut(s) 161, 338
ScrFI CCNGG 7 cut(s) 57, 171, 193, 545, 786, 864, 910
SduI GDGCHC 3 cut(s) 65, 102, 1024
SfaNI GCATC 3 cut(s) 244, 287, 1078
SfcI CTRYAG 2 cut(s) 436, 885
SinI GGWCC 2 cut(s) 320, 590
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 2 cut(s) 104, 1117
SmoI CTYRAG 2 cut(s) 104, 1117
SseBI AGGCCT 2 cut(s) 47, 929
SsiI CCGC 2 cut(s) 578, 759
SspI AATATT 1 cut(s) 1030
SspMI CTAG 3 cut(s) 69, 339, 1143
SstI GAGCTC 1 cut(s) 65
StuI AGGCCT 2 cut(s) 47, 929
StyD4I CCNGG 7 cut(s) 55, 169, 191, 543, 784, 862, 908
TaaI ACNGT 5 cut(s) 889, 920, 1066, 1112, 1196
TaiI ACGT 3 cut(s) 351, 755, 881
TaqI TCGA 3 cut(s) 128, 414, 1207
TatI WGTACW 3 cut(s) 293, 675, 915
TfiI GAWTC 2 cut(s) 450, 791
Tru1I TTAA 4 cut(s) 242, 396, 1128, 1219
Tru9I TTAA 4 cut(s) 242, 396, 1128, 1219
TscAI CASTG 4 cut(s) 652, 925, 1071, 1098
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 3 cut(s) 302, 463, 1214
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 2 cut(s) 567, 1166
TspRI CASTG 4 cut(s) 652, 925, 1071, 1098
Tth111I GACNNNGTC 1 cut(s) 995
Van91I CCANNNNNTGG 1 cut(s) 477
VneI GTGCAC 1 cut(s) 1020
VpaK11BI GGWCC 2 cut(s) 320, 590
XapI RAATTY 5 cut(s) 383, 489, 507, 709, 1124
XceI RCATGY 2 cut(s) 309, 1044
XcmI CCANNNNNNNNNTGG 1 cut(s) 474
XmiI GTMKAC 2 cut(s) 881, 959
XspI CTAG 3 cut(s) 69, 339, 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.