AT1G74320

choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002182
Physical Location & Seq
Forward (+)
27940594 .. 27943204
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74320.1

Sequence Viewer

Length: 1053 bp
ATGACGATGGGTGGTACTGAAAAGAATGTAGAGAATAAACAATATCGTTTGCCTCGAGAAGTCAAGGAAGCTTTACAAGCAATAGCATCAGAGTGGGAAGATGTGATTGATTCAAAGGCTCTGCAAGTTATTCCTTTAAAAGGTGCAATGACAAATGAGGTGTTTCAGATCAAATGGCCAACTAGGGAAAAAGGTCCTTCTCGTAAGGTTTTGGTTAGGATTTATGGTGAAGGTGTTGAGATTTTCTTTGATCGTGAGGATGAGATCAGGACATTTGAGTTCATGTCGAAGCATGGTCATGGACCTTTGTTGTTAGGTCGGTTTGGTAATGGTCGTATCGAAGAGTTCCTCCATGCACGGACCCTATCTGCTTGTGACTTGCGTGATCCTGAAATATCTGGTCGAATAGCAACTAGGATGAAGGAGTTTCATGGTCTTGAAATGCCTGGTGCGAAAAAGGCTTTGCTTTGGGACAGATTACGAAATTGGCTCACTGCATGCAAGAGACTGGCTTCACCAGAAGAAGCTAAGTCCTTTCGTCTCGATGTTATGGAGATGGAAATTAATATGCTTGAGAAATCTCTCTTTGACAATGATGAAAATATTGGATTTTGCCACAATGATTTACAGTATGGGAACATCATGATGGATGAAGAGACCAAAGCAATAACCATCATTGATTATGAATATTCATGCTATAATCCTGTGGCTTATGACATTGCCAATCACTTCTGTGAGATGGCTGCCGATTACCACACTGAAACACCTCACATCATGGATTATAGTAAATACCCTGGTGTGGAGGAGCGACAAAGATTTCTGAAAACATATATGAGTTATTCAGATGAAAAGCCCAGCGATACTATGGTCAAAAAACTCCTAGAAGACGTTGAGAAATATACACTTGCTAGTCATCTAATTTGGGGTTTATGGGGAATTATATCGGAACATGTGAATGAAATCGACTTTGACTACATGGAGTATGCAAGACAAAGGTTCGAGCAGTATTGGTTAACAAAGCCGAGGCTCCTTGCAGCTTCCGAACACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

350

Amino Acids

41.02

Weight (kDa)

5.37

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_kinase PF01633 44 - 337 1.5e-109 Choline/ethanolamine kinase
APH PF01636 139 - 244 1.2e-12 Phosphotransferase enzyme family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 1 cut(s) 176
AfaI GTAC 1 cut(s) 16
AfiI CCNNNNNNNGG 2 cut(s) 140, 799
AflIII ACRYGT 1 cut(s) 949
AgsI TTSAA 2 cut(s) 114, 440
AjnI CCWGG 2 cut(s) 445, 793
AleI CACNNNNGTG 1 cut(s) 732
AluBI AGCT 3 cut(s) 71, 527, 1037
AluI AGCT 3 cut(s) 71, 527, 1037
Alw26I GTCTC 3 cut(s) 499, 545, 650
AlwI GGATC 1 cut(s) 380
Ama87I CYCGRG 1 cut(s) 54
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 2 cut(s) 743, 1034
AseI ATTAAT 1 cut(s) 564
AspS9I GGNCC 3 cut(s) 194, 302, 360
AsuHPI GGTGA 2 cut(s) 239, 507
AvaI CYCGRG 1 cut(s) 54
AvaII GGWCC 3 cut(s) 194, 302, 360
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
BalI TGGCCA 1 cut(s) 178
BbsI GAAGAC 1 cut(s) 891
BbvI GCAGC 2 cut(s) 730, 1046
BccI CCATC 4 cut(s) 550, 640, 680, 733
BciT130I CCWGG 2 cut(s) 447, 795
BcoDI GTCTC 3 cut(s) 499, 545, 650
BfaI CTAG 4 cut(s) 183, 414, 881, 909
BisI GCNGC 2 cut(s) 744, 1035
BlsI GCNGC 2 cut(s) 745, 1036
Bme1390I CCNGG 2 cut(s) 447, 795
Bme18I GGWCC 3 cut(s) 194, 302, 360
BmeT110I CYCGRG 1 cut(s) 54
BmgT120I GGNCC 3 cut(s) 194, 302, 360
BmiI GGNNCC 2 cut(s) 362, 1028
BmrFI CCNGG 2 cut(s) 447, 795
BmsI GCATC 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 891
BpuEI CTTGAG 1 cut(s) 593
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 2 cut(s) 793, 1022
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 799
Bse1I ACTGG 1 cut(s) 513
Bse3DI GCAATG 2 cut(s) 153, 717
BseBI CCWGG 2 cut(s) 447, 795
BseDI CCNNGG 2 cut(s) 793, 1022
BseGI GGATG 3 cut(s) 265, 423, 655
BseLI CCNNNNNNNGG 2 cut(s) 140, 799
BseMI GCAATG 2 cut(s) 153, 717
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 1 cut(s) 818
BseXI GCAGC 2 cut(s) 730, 1046
BseYI CCCAGC 1 cut(s) 854
BshFI GGCC 1 cut(s) 178
BsiHKCI CYCGRG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 2 cut(s) 140, 799
BsmAI GTCTC 3 cut(s) 499, 545, 650
BsmBI CGTCTC 1 cut(s) 545
BsmFI GGGAC 1 cut(s) 485
BsnI GGCC 1 cut(s) 178
Bso31I GGTCTC 1 cut(s) 650
BsoBI CYCGRG 1 cut(s) 54
Bsp143I GATC 4 cut(s) 168, 250, 264, 385
BspANI GGCC 1 cut(s) 178
BspHI TCATGA 1 cut(s) 642
BspLI GGNNCC 2 cut(s) 362, 1028
BspPI GGATC 1 cut(s) 380
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 2 cut(s) 153, 717
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 2 cut(s) 793, 1022
BssMI GATC 4 cut(s) 168, 250, 264, 385
Bst2UI CCWGG 2 cut(s) 447, 795
Bst4CI ACNGT 1 cut(s) 630
Bst6I CTCTTC 2 cut(s) 336, 648
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 1 cut(s) 528
BstENI CCTNNNNNAGG 1 cut(s) 138
BstF5I GGATG 3 cut(s) 265, 423, 655
BstKTI GATC 4 cut(s) 171, 253, 267, 388
BstMAI GTCTC 3 cut(s) 499, 545, 650
BstMBI GATC 4 cut(s) 168, 250, 264, 385
BstMWI GCNNNNNNNGC 2 cut(s) 77, 458
BstNI CCWGG 2 cut(s) 447, 795
BstNSI RCATGY 2 cut(s) 501, 953
BstSCI CCNGG 2 cut(s) 445, 793
BstV1I GCAGC 2 cut(s) 730, 1046
BstV2I GAAGAC 1 cut(s) 891
BsuRI GGCC 1 cut(s) 178
BtsCI GGATG 3 cut(s) 265, 423, 655
BtsI GCAGTG 1 cut(s) 492
BtsIMutI CAGTG 2 cut(s) 492, 756
Cac8I GCNNGC 1 cut(s) 499
CciI TCATGA 1 cut(s) 642
Cfr13I GGNCC 3 cut(s) 194, 302, 360
Csp6I GTAC 1 cut(s) 15
CviQI GTAC 1 cut(s) 15
DdeI CTNAG 1 cut(s) 528
DpnI GATC 4 cut(s) 170, 252, 266, 387
DpnII GATC 4 cut(s) 168, 250, 264, 385
DraI TTTAAA 1 cut(s) 138
EaeI YGGCCR 1 cut(s) 176
Eam1104I CTCTTC 2 cut(s) 336, 648
EarI CTCTTC 2 cut(s) 336, 648
Eco31I GGTCTC 1 cut(s) 650
Eco47I GGWCC 3 cut(s) 194, 302, 360
Eco88I CYCGRG 1 cut(s) 54
EcoNI CCTNNNNNAGG 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 445, 793
Esp3I CGTCTC 1 cut(s) 545
FaqI GGGAC 1 cut(s) 485
Fnu4HI GCNGC 2 cut(s) 744, 1035
FokI GGATG 3 cut(s) 272, 430, 662
Fsp4HI GCNGC 2 cut(s) 744, 1035
FspBI CTAG 4 cut(s) 183, 414, 881, 909
GluI GCNGC 2 cut(s) 744, 1035
GsaI CCCAGC 1 cut(s) 858
HaeIII GGCC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 1014
HindII GTYRAC 1 cut(s) 1014
HindIII AAGCTT 1 cut(s) 69
HinfI GANTC 1 cut(s) 110
HpaI GTTAAC 1 cut(s) 1014
HphI GGTGA 2 cut(s) 239, 507
Hpy166II GTNNAC 1 cut(s) 1014
Hpy188I TCNGA 6 cut(s) 91, 168, 822, 844, 946, 1042
Hpy188III TCNNGA 7 cut(s) 56, 254, 268, 389, 437, 542, 643
Hpy8I GTNNAC 1 cut(s) 1014
HpyAV CCTTC 3 cut(s) 207, 224, 415
HpyCH4III ACNGT 1 cut(s) 630
HpyCH4IV ACGT 1 cut(s) 888
HpyCH4V TGCA 7 cut(s) 124, 146, 356, 497, 501, 986, 1034
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 458
HpyF3I CTNAG 1 cut(s) 528
HpySE526I ACGT 1 cut(s) 888
KspAI GTTAAC 1 cut(s) 1014
Kzo9I GATC 4 cut(s) 168, 250, 264, 385
LmnI GCTCC 2 cut(s) 805, 1032
Lsp1109I GCAGC 2 cut(s) 730, 1046
LweI GCATC 1 cut(s) 95
MaeI CTAG 4 cut(s) 183, 414, 881, 909
MaeII ACGT 1 cut(s) 888
MaeIII GTNAC 1 cut(s) 374
MalI GATC 4 cut(s) 170, 252, 266, 387
MboI GATC 4 cut(s) 168, 250, 264, 385
MboII GAAGA 5 cut(s) 110, 353, 533, 665, 896
MlsI TGGCCA 1 cut(s) 178
MluCI AATT 4 cut(s) 484, 561, 918, 936
MluNI TGGCCA 1 cut(s) 178
MnlI CCTC 7 cut(s) 63, 151, 250, 359, 777, 796, 1017
Mox20I TGGCCA 1 cut(s) 178
MscI TGGCCA 1 cut(s) 178
MseI TTAA 3 cut(s) 137, 564, 1013
MslI CAYNNNNRTG 5 cut(s) 91, 297, 644, 732, 954
Msp20I TGGCCA 1 cut(s) 178
MspR9I CCNGG 2 cut(s) 447, 795
MvaI CCWGG 2 cut(s) 447, 795
MwoI GCNNNNNNNGC 2 cut(s) 77, 458
NdeII GATC 4 cut(s) 168, 250, 264, 385
NlaIV GGNNCC 2 cut(s) 362, 1028
NmeAIII GCCGAG 1 cut(s) 1047
NmuCI GTSAC 1 cut(s) 374
NspI RCATGY 2 cut(s) 501, 953
OliI CACNNNNGTG 1 cut(s) 732
PaeI GCATGC 1 cut(s) 501
PaeR7I CTCGAG 1 cut(s) 54
PagI TCATGA 1 cut(s) 642
PciI ACATGT 1 cut(s) 949
PcsI WCGNNNNNNNCGW 1 cut(s) 52
PfeI GAWTC 1 cut(s) 110
PkrI GCNGC 2 cut(s) 745, 1036
PpuMI RGGWCCY 1 cut(s) 194
PscI ACATGT 1 cut(s) 949
PshBI ATTAAT 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 194
Psp6I CCWGG 2 cut(s) 445, 793
PspFI CCCAGC 1 cut(s) 854
PspGI CCWGG 2 cut(s) 445, 793
PspN4I GGNNCC 2 cut(s) 362, 1028
PspPI GGNCC 3 cut(s) 194, 302, 360
PspPPI RGGWCCY 1 cut(s) 194
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
RseI CAYNNNNRTG 5 cut(s) 91, 297, 644, 732, 954
SaqAI TTAA 3 cut(s) 137, 564, 1013
SatI GCNGC 2 cut(s) 744, 1035
Sau3AI GATC 4 cut(s) 168, 250, 264, 385
Sau96I GGNCC 3 cut(s) 194, 302, 360
ScrFI CCNGG 2 cut(s) 447, 795
SfaNI GCATC 1 cut(s) 95
Sfr274I CTCGAG 1 cut(s) 54
SinI GGWCC 3 cut(s) 194, 302, 360
SlaI CTCGAG 1 cut(s) 54
SmiMI CAYNNNNRTG 5 cut(s) 91, 297, 644, 732, 954
SmlI CTYRAG 2 cut(s) 54, 572
SmoI CTYRAG 2 cut(s) 54, 572
SphI GCATGC 1 cut(s) 501
Sse9I AATT 4 cut(s) 484, 561, 918, 936
SspI AATATT 2 cut(s) 604, 689
SspMI CTAG 4 cut(s) 183, 414, 881, 909
StyD4I CCNGG 2 cut(s) 445, 793
TaaI ACNGT 1 cut(s) 630
TaiI ACGT 1 cut(s) 891
TaqI TCGA 7 cut(s) 55, 287, 339, 403, 543, 963, 999
TasI AATT 4 cut(s) 484, 561, 918, 936
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 3 cut(s) 137, 564, 1013
Tru9I TTAA 3 cut(s) 137, 564, 1013
TscAI CASTG 2 cut(s) 499, 763
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 2 cut(s) 743, 1034
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 9 cut(s) 271, 419, 434, 612, 666, 681, 699, 861, 972
TspGWI ACGGA 1 cut(s) 373
TspRI CASTG 2 cut(s) 499, 763
VpaK11BI GGWCC 3 cut(s) 194, 302, 360
VspI ATTAAT 1 cut(s) 564
XagI CCTNNNNNAGG 1 cut(s) 138
XceI RCATGY 2 cut(s) 501, 953
XcmI CCANNNNNNNNNTGG 1 cut(s) 862
XhoI CTCGAG 1 cut(s) 54
XspI CTAG 4 cut(s) 183, 414, 881, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.