FvH4_7g03960

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002182
Physical Location & Seq
Reverse (-)
4547831 .. 4552299
4469 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03960.t1

Sequence Viewer

Length: 1077 bp
ATGGGTGCCGTGGTGAATTCCATAGATAGAGACGACCGTATTCCAAAAGAAGCCAAGGAAATTCTACAATCATTGGCTACCAAGTGGGAAAATGTAGATGATTCAACTGCGCTGCAGGTGATCCCTCTCAAAGGCGCAATGACCAACGAGGTGTTCCAGATAAAGTGGCCGACAAGTACTGGGGAAGTCTCATGCAAGGTTCTGGTTAGGATTTACGGTGAGGGTGTTGAGGTGTTCTTTGACAGGGATAATGAGATCCGGACTTTTGAATACATGTCAAAGAATGGTCAGGGGCCTCGTCTGCTCGGGCGTTTTCCTAATGGCCGAATTGAAGAGTTCATCCATGCACGGACACTTTCAGCATCTGATCTACGTGATCCAGATATATCTGCTCTTATAGCTACTAAAATGAAGGAGTTCCATGATCTTGAGATGCCTGGTCCAAAGGATGTTGTCCTCTGGGGCAGATTGAGAAATTGGCTCAGTACGGCCAAGAAATTGTGTAGTCCAGAAGAAGCTAATGAATTTCGGTTAGATTCCATTGAAGAAGAGATCTCTATACTGGAAAAGGAGCTATCAGTGCCTAATCAGCAGATGGGTTTCTGTCACAATGATTTACAGTATGGTAATATAATGATTGAGGAAGAGAGTAAATCGATAACGATAATTGACTATGAGTATGCAAGTTATAATCCTATTGCATTTGACATAGCAAACCATTTCTGTGAGATGGCAGCTGATTATCACACAGATACCCCTCATATTTTGGACTACAATAAATATCCTGGCATAGAGGAGCGCAAAAGGTTTGTTCGTGTGTATCTGAGTTCTTCAGGAAATCAACCTAGCGATAATGACTTAGAGCAGCTAGTTCAAGAAGTTGAGAAGTATACCCTTCCAGATCATCTGTTCTGGGGATTATGGGGAATAATATCGGAACATGTGAATGATATTGATTTTGACTACTTGGAATATGCAAAGCAGAGGTTTCAGCAGTACTGGAGAACCAAGGCTTCACTATTGGGTTCTGTTGGATCTTCACCAGACACAGGGACTGATGGTAACTGTGCTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

359

Amino Acids

41.01

Weight (kDa)

4.75

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_kinase PF01633 41 - 334 1.4e-101 Choline/ethanolamine kinase
APH PF01636 44 - 241 4.6e-11 Phosphotransferase enzyme family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 690
AarI CACCTGC 1 cut(s) 106
Acc36I ACCTGC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 5
AccI GTMKAC 1 cut(s) 890
AccIII TCCGGA 1 cut(s) 258
AclWI GGATC 4 cut(s) 115, 250, 371, 1042
AcoI YGGCCR 3 cut(s) 167, 322, 489
AcsI RAATTY 3 cut(s) 16, 60, 524
AcuI CTGAAG 1 cut(s) 816
AfaI GTAC 3 cut(s) 178, 487, 998
AfiI CCNNNNNNNGG 2 cut(s) 131, 1049
AflIII ACRYGT 2 cut(s) 273, 940
AgsI TTSAA 5 cut(s) 105, 269, 332, 545, 875
AjnI CCWGG 2 cut(s) 436, 784
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AluBI AGCT 5 cut(s) 401, 518, 574, 737, 868
AluI AGCT 5 cut(s) 401, 518, 574, 737, 868
Alw21I GWGCWC 1 cut(s) 1072
Alw26I GTCTC 2 cut(s) 24, 193
AlwI GGATC 4 cut(s) 115, 250, 371, 1042
AlwNI CAGNNNCTG 2 cut(s) 365, 1055
Ama87I CYCGRG 1 cut(s) 305
Aor13HI TCCGGA 1 cut(s) 258
AoxI GGCC 4 cut(s) 167, 293, 322, 489
ApeKI GCWGC 3 cut(s) 112, 734, 865
ApoI RAATTY 3 cut(s) 16, 60, 524
Asp700I GAANNNNTTC 1 cut(s) 416
AspLEI GCGC 3 cut(s) 112, 137, 801
AspS9I GGNCC 2 cut(s) 293, 440
AsuHPI GGTGA 4 cut(s) 25, 130, 230, 1032
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 440
BanI GGYRCC 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 1072
BbvI GCAGC 3 cut(s) 99, 746, 877
BccI CCATC 3 cut(s) 589, 724, 1052
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 2 cut(s) 438, 786
BcoDI GTCTC 2 cut(s) 24, 193
BfaI CTAG 2 cut(s) 846, 869
BfmI CTRYAG 1 cut(s) 113
BfuAI ACCTGC 1 cut(s) 106
BglII AGATCT 1 cut(s) 552
BisI GCNGC 3 cut(s) 113, 735, 866
BlsI GCNGC 3 cut(s) 114, 736, 867
BmcAI AGTACT 2 cut(s) 178, 998
Bme1390I CCNGG 2 cut(s) 438, 786
Bme18I GGWCC 1 cut(s) 440
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 293, 440
BmiI GGNNCC 2 cut(s) 7, 294
BmrFI CCNGG 2 cut(s) 438, 786
BmrI ACTGGG 1 cut(s) 189
BmsI GCATC 2 cut(s) 371, 423
BmuI ACTGGG 1 cut(s) 189
BpmI CTGGAG 1 cut(s) 1021
BpuEI CTTGAG 1 cut(s) 449
Bsa29I ATCGAT 1 cut(s) 656
BsaAI YACGTR 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 9, 54, 1008
BsaWI WCCGGW 1 cut(s) 258
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 1049
Bse1I ACTGG 3 cut(s) 184, 567, 1004
Bse3DI GCAATG 1 cut(s) 144
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 2 cut(s) 438, 786
BseCI ATCGAT 1 cut(s) 656
BseDI CCNNGG 3 cut(s) 9, 54, 1008
BseGI GGATG 2 cut(s) 339, 454
BseLI CCNNNNNNNGG 2 cut(s) 131, 1049
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 2 cut(s) 496, 815
BseNI ACTGG 3 cut(s) 184, 567, 1004
BseRI GAGGAG 1 cut(s) 809
BseXI GCAGC 3 cut(s) 99, 746, 877
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 4 cut(s) 169, 295, 324, 491
BshNI GGYRCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 656
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 1072
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 1 cut(s) 259
BslFI GGGAC 1 cut(s) 1066
BslI CCNNNNNNNGG 2 cut(s) 131, 1049
BsmAI GTCTC 2 cut(s) 24, 193
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 1066
BsnI GGCC 4 cut(s) 169, 295, 324, 491
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 1072
Bsp13I TCCGGA 1 cut(s) 258
Bsp143I GATC 8 cut(s) 120, 255, 367, 376, 424, 552, 901, 1034
BspANI GGCC 4 cut(s) 169, 295, 324, 491
BspCNI CTCAG 2 cut(s) 495, 816
BspDI ATCGAT 1 cut(s) 656
BspEI TCCGGA 1 cut(s) 258
BspLI GGNNCC 2 cut(s) 7, 294
BspMAI CTGCAG 1 cut(s) 117
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 4 cut(s) 115, 250, 371, 1042
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 144
BsrI ACTGG 3 cut(s) 184, 567, 1004
BssECI CCNNGG 3 cut(s) 9, 54, 1008
BssMI GATC 8 cut(s) 120, 255, 367, 376, 424, 552, 901, 1034
BssNAI GTATAC 1 cut(s) 891
BssT1I CCWWGG 2 cut(s) 54, 1008
Bst1107I GTATAC 1 cut(s) 891
Bst2UI CCWGG 2 cut(s) 438, 786
Bst4CI ACNGT 4 cut(s) 38, 218, 621, 1067
Bst6I CTCTTC 3 cut(s) 327, 543, 639
BstBAI YACGTR 1 cut(s) 374
BstDEI CTNAG 3 cut(s) 482, 824, 859
BstDSI CCRYGG 1 cut(s) 9
BstENI CCTNNNNNAGG 1 cut(s) 129
BstF5I GGATG 2 cut(s) 339, 454
BstHHI GCGC 3 cut(s) 112, 137, 801
BstKTI GATC 8 cut(s) 123, 258, 370, 379, 427, 555, 904, 1037
BstMAI GTCTC 2 cut(s) 24, 193
BstMBI GATC 8 cut(s) 120, 255, 367, 376, 424, 552, 901, 1034
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 4 cut(s) 301, 398, 580, 589
BstNI CCWGG 2 cut(s) 438, 786
BstNSI RCATGY 2 cut(s) 277, 944
BstSCI CCNGG 2 cut(s) 436, 784
BstSFI CTRYAG 1 cut(s) 113
BstV1I GCAGC 3 cut(s) 99, 746, 877
BstX2I RGATCY 3 cut(s) 255, 552, 1034
BstYI RGATCY 3 cut(s) 255, 552, 1034
BstZ17I GTATAC 1 cut(s) 891
Bsu15I ATCGAT 1 cut(s) 656
BsuRI GGCC 4 cut(s) 169, 295, 324, 491
BsuTUI ATCGAT 1 cut(s) 656
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 2 cut(s) 339, 454
BtsIMutI CAGTG 1 cut(s) 585
BveI ACCTGC 1 cut(s) 106
CaiI CAGNNNCTG 2 cut(s) 365, 1055
CfoI GCGC 3 cut(s) 112, 137, 801
Cfr13I GGNCC 2 cut(s) 293, 440
ClaI ATCGAT 1 cut(s) 656
Csp6I GTAC 3 cut(s) 177, 486, 997
CviAII CATG 5 cut(s) 192, 274, 344, 422, 941
CviQI GTAC 3 cut(s) 177, 486, 997
DdeI CTNAG 3 cut(s) 482, 824, 859
DpnI GATC 8 cut(s) 122, 257, 369, 378, 426, 554, 903, 1036
DpnII GATC 8 cut(s) 120, 255, 367, 376, 424, 552, 901, 1034
EaeI YGGCCR 3 cut(s) 167, 322, 489
Eam1104I CTCTTC 3 cut(s) 327, 543, 639
EarI CTCTTC 3 cut(s) 327, 543, 639
Eco130I CCWWGG 2 cut(s) 54, 1008
Eco47I GGWCC 1 cut(s) 440
Eco57I CTGAAG 1 cut(s) 816
Eco88I CYCGRG 1 cut(s) 305
EcoNI CCTNNNNNAGG 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 293
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 436, 784
EcoT14I CCWWGG 2 cut(s) 54, 1008
ErhI CCWWGG 2 cut(s) 54, 1008
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 5 cut(s) 195, 277, 347, 425, 944
FaqI GGGAC 1 cut(s) 1066
FatI CATG 5 cut(s) 191, 273, 343, 421, 940
FblI GTMKAC 1 cut(s) 890
Fnu4HI GCNGC 3 cut(s) 113, 735, 866
FokI GGATG 2 cut(s) 326, 461
Fsp4HI GCNGC 3 cut(s) 113, 735, 866
FspBI CTAG 2 cut(s) 846, 869
GlaI GCGC 3 cut(s) 111, 136, 800
GluI GCNGC 3 cut(s) 113, 735, 866
GsuI CTGGAG 1 cut(s) 1021
HaeIII GGCC 4 cut(s) 169, 295, 324, 491
HapII CCGG 1 cut(s) 259
HhaI GCGC 3 cut(s) 112, 137, 801
Hin1II CATG 5 cut(s) 195, 277, 347, 425, 944
Hin6I GCGC 3 cut(s) 110, 135, 799
HinP1I GCGC 3 cut(s) 110, 135, 799
HinfI GANTC 2 cut(s) 101, 536
HpaII CCGG 1 cut(s) 259
HphI GGTGA 4 cut(s) 25, 130, 230, 1032
Hpy166II GTNNAC 1 cut(s) 891
Hpy188I TCNGA 3 cut(s) 367, 825, 937
Hpy188III TCNNGA 8 cut(s) 157, 259, 380, 428, 509, 834, 875, 899
Hpy8I GTNNAC 1 cut(s) 891
HpyAV CCTTC 2 cut(s) 406, 905
HpyCH4III ACNGT 4 cut(s) 38, 218, 621, 1067
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 6 cut(s) 115, 195, 347, 683, 701, 977
HpyF10VI GCNNNNNNNGC 4 cut(s) 301, 398, 580, 589
HpyF3I CTNAG 3 cut(s) 482, 824, 859
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 5 cut(s) 195, 277, 347, 425, 944
HspAI GCGC 3 cut(s) 110, 135, 799
Kpn2I TCCGGA 1 cut(s) 258
Kzo9I GATC 8 cut(s) 120, 255, 367, 376, 424, 552, 901, 1034
LmnI GCTCC 2 cut(s) 571, 796
Lsp1109I GCAGC 3 cut(s) 99, 746, 877
LweI GCATC 2 cut(s) 371, 423
MaeI CTAG 2 cut(s) 846, 869
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 2 cut(s) 605, 1061
MalI GATC 8 cut(s) 122, 257, 369, 378, 426, 554, 903, 1036
MboI GATC 8 cut(s) 120, 255, 367, 376, 424, 552, 901, 1034
MboII GAAGA 7 cut(s) 344, 524, 557, 560, 656, 822, 1029
MflI RGATCY 3 cut(s) 255, 552, 1034
MhlI GDGCHC 1 cut(s) 1072
MluCI AATT 7 cut(s) 16, 60, 327, 475, 497, 524, 666
MmeI TCCRAC 1 cut(s) 1012
MroI TCCGGA 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 416
MslI CAYNNNNRTG 2 cut(s) 723, 945
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 1 cut(s) 259
MspR9I CCNGG 2 cut(s) 438, 786
MvaI CCWGG 2 cut(s) 438, 786
MwoI GCNNNNNNNGC 4 cut(s) 301, 398, 580, 589
NdeII GATC 8 cut(s) 120, 255, 367, 376, 424, 552, 901, 1034
NlaIII CATG 5 cut(s) 195, 277, 347, 425, 944
NlaIV GGNNCC 2 cut(s) 7, 294
NmuCI GTSAC 1 cut(s) 605
NspI RCATGY 2 cut(s) 277, 944
PaqCI CACCTGC 1 cut(s) 106
PciI ACATGT 2 cut(s) 273, 940
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 2 cut(s) 101, 536
PkrI GCNGC 3 cut(s) 114, 736, 867
Ppu21I YACGTR 1 cut(s) 374
PscI ACATGT 2 cut(s) 273, 940
PsiI TTATAA 1 cut(s) 690
Psp6I CCWGG 2 cut(s) 436, 784
PspGI CCWGG 2 cut(s) 436, 784
PspN4I GGNNCC 2 cut(s) 7, 294
PspPI GGNCC 2 cut(s) 293, 440
PstI CTGCAG 1 cut(s) 117
PstNI CAGNNNCTG 2 cut(s) 365, 1055
PsuI RGATCY 3 cut(s) 255, 552, 1034
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 3 cut(s) 178, 487, 998
RsaNI GTAC 3 cut(s) 177, 486, 997
RseI CAYNNNNRTG 2 cut(s) 723, 945
SatI GCNGC 3 cut(s) 113, 735, 866
Sau3AI GATC 8 cut(s) 120, 255, 367, 376, 424, 552, 901, 1034
Sau96I GGNCC 2 cut(s) 293, 440
ScaI AGTACT 2 cut(s) 178, 998
ScrFI CCNGG 2 cut(s) 438, 786
SduI GDGCHC 1 cut(s) 1072
SfaNI GCATC 2 cut(s) 371, 423
SfcI CTRYAG 1 cut(s) 113
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 2 cut(s) 723, 945
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 7 cut(s) 16, 60, 327, 475, 497, 524, 666
SspMI CTAG 2 cut(s) 846, 869
StyD4I CCNGG 2 cut(s) 436, 784
StyI CCWWGG 2 cut(s) 54, 1008
TaaI ACNGT 4 cut(s) 38, 218, 621, 1067
TaiI ACGT 1 cut(s) 376
TaqI TCGA 1 cut(s) 656
TasI AATT 7 cut(s) 16, 60, 327, 475, 497, 524, 666
TatI WGTACW 2 cut(s) 176, 996
TfiI GAWTC 2 cut(s) 101, 536
TscAI CASTG 1 cut(s) 585
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 3 cut(s) 112, 734, 865
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 3 cut(s) 328, 425, 537
TspGWI ACGGA 1 cut(s) 364
TspRI CASTG 1 cut(s) 585
VpaK11BI GGWCC 1 cut(s) 440
XagI CCTNNNNNAGG 1 cut(s) 129
XapI RAATTY 3 cut(s) 16, 60, 524
XceI RCATGY 2 cut(s) 277, 944
XmiI GTMKAC 1 cut(s) 890
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 2 cut(s) 846, 869
ZrmI AGTACT 2 cut(s) 178, 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.