RchiOBHm_Chr1g0327661

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002182
Physical Location & Seq
Reverse (-)
16597398 .. 16602452
5055 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55716

Sequence Viewer

Length: 1077 bp
ATGGGTGCCGTGGTGAATTCCATAGATAAAGACGACCGTATTCCAAAAGAAGCCAAGGAGATTCTACAATCATTGGCTACCGAGTGGGAAAATGTAGCTGATTCCACTGCCTTGCAGGTCATCCCTCTCAAGGGTGCAATGACTAACGAGGTGTTCGAAATAAAGTGGCCAACAAGCACCGGGGAAGTCTCGCGAAAGGTTCTGGTTAGGATTTACGGTGAGGGTGTTGAGGTGTTCTTTGACAGGGATAATGAGATCCGAACATTTGAATACATGTCAAAGAACGGTCAGGGACCTCGTCTGCTTGGCCGTTTTCCTAATGGCCGAGTTGAAGAGTTCATCCATGCACGGACACTTTCAGCATCTGATCTACGTGATCCAGATATATCTGCTCTTATAGCTACTAAAATGAAGGAATTCCATGATCTTCAGATGCCCGGTCCAAAGGATGTCGTCCTCTGGGGCAGATTGAGAAATTGGCTGAGTACAGCCAAGAGATTGTGTAGTCCAGAAGAAGCTAATGAATTTCAGTTAGATTCCATTGAAAAGGAAATCTCTATACTGGAGAAGGAGCTCTCAGTACTGAATCAGCAGTTAGGTTTCTGCCACAATGATTTACAGTATGGAAACATAATGATTGAGGAAGAGAGTAAAACGATAACGATAATTGACTATGAGTATGCAAGTTATAATCCTGTTGCATTTGACATAGCAAACCATTTCTGTGAGATGGCGGCTGACTATCACACAGATACCCCTCATATTTTAGACTACAGTAAATATCCTGGCTTAGAGGAGCGCAAGAGGTTTGTTCGTATGTATCTGAATTCTTCAGGCAATCAACCTATTGACAAAGAAGTGGAGCAGCTAGTTGAAGATGTTGAGAAGTATACCCTTGCAGACCATCTGTTCTGGGGCTTATGGGGAATAATATCGGAACATGTGAATGATATTGATTTTGACTACATGCAATATGCAAAGCAGAGATTTCAACAGTACTGGAGAACCAAGGCTTTGCTATTGGGTTCTGTTGGATCTTCCCCAGACACAGGGACTGATGGTAACCGTACTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

358

Amino Acids

41.13

Weight (kDa)

4.87

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_kinase PF01633 41 - 334 5.4e-103 Choline/ethanolamine kinase
APH PF01636 66 - 241 6.7e-11 Phosphotransferase enzyme family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 690
Acc36I ACCTGC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 5
AccI GTMKAC 1 cut(s) 890
AccII CGCG 1 cut(s) 193
AciI CCGC 1 cut(s) 734
AclWI GGATC 3 cut(s) 250, 371, 1042
AcoI YGGCCR 3 cut(s) 167, 307, 322
AcsI RAATTY 4 cut(s) 16, 416, 524, 826
AcuI CTGAAG 2 cut(s) 413, 816
AfaI GTAC 4 cut(s) 487, 582, 998, 1069
AfiI CCNNNNNNNGG 3 cut(s) 130, 131, 1049
AflIII ACRYGT 2 cut(s) 273, 940
AgsI TTSAA 5 cut(s) 269, 332, 545, 875, 992
AjnI CCWGG 1 cut(s) 784
AluBI AGCT 5 cut(s) 98, 401, 518, 574, 868
AluI AGCT 5 cut(s) 98, 401, 518, 574, 868
Alw21I GWGCWC 1 cut(s) 576
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 3 cut(s) 250, 371, 1042
AlwNI CAGNNNCTG 2 cut(s) 365, 1055
AoxI GGCC 3 cut(s) 167, 307, 322
ApeKI GCWGC 1 cut(s) 865
ApoI RAATTY 4 cut(s) 16, 416, 524, 826
Asp700I GAANNNNTTC 1 cut(s) 416
AspLEI GCGC 1 cut(s) 801
AspS9I GGNCC 2 cut(s) 293, 440
AsuC2I CCSGG 2 cut(s) 181, 438
AsuHPI GGTGA 2 cut(s) 25, 230
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 2 cut(s) 293, 440
BalI TGGCCA 1 cut(s) 169
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 576
Bbv12I GWGCWC 1 cut(s) 576
BbvI GCAGC 1 cut(s) 877
BccI CCATC 3 cut(s) 724, 912, 1052
BceAI ACGGC 1 cut(s) 294
BciT130I CCWGG 1 cut(s) 786
BcnI CCSGG 2 cut(s) 181, 438
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 1 cut(s) 869
BfmI CTRYAG 1 cut(s) 772
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 2 cut(s) 735, 866
BlsI GCNGC 2 cut(s) 736, 867
BmcAI AGTACT 2 cut(s) 582, 998
Bme1390I CCNGG 3 cut(s) 181, 438, 786
Bme18I GGWCC 2 cut(s) 293, 440
BmgT120I GGNCC 2 cut(s) 293, 440
BmiI GGNNCC 2 cut(s) 7, 294
BmrFI CCNGG 3 cut(s) 181, 438, 786
BmsI GCATC 2 cut(s) 371, 423
BpmI CTGGAG 2 cut(s) 584, 1021
Bpu14I TTCGAA 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 2 cut(s) 181, 438
BsaAI YACGTR 1 cut(s) 374
BsaJI CCNNGG 4 cut(s) 9, 54, 180, 1008
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 131, 1049
Bse1I ACTGG 2 cut(s) 567, 1004
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 1 cut(s) 786
BseDI CCNNGG 4 cut(s) 9, 54, 180, 1008
BseGI GGATG 3 cut(s) 120, 339, 454
BseLI CCNNNNNNNGG 3 cut(s) 130, 131, 1049
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 2 cut(s) 473, 591
BseNI ACTGG 2 cut(s) 567, 1004
BseRI GAGGAG 1 cut(s) 809
BseXI GCAGC 1 cut(s) 877
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 3 cut(s) 169, 309, 324
BshNI GGYRCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 576
BsiSI CCGG 2 cut(s) 180, 438
BslFI GGGAC 2 cut(s) 306, 1066
BslI CCNNNNNNNGG 3 cut(s) 130, 131, 1049
BsmAI GTCTC 1 cut(s) 193
BsmFI GGGAC 2 cut(s) 306, 1066
BsnI GGCC 3 cut(s) 169, 309, 324
Bsp119I TTCGAA 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 576
Bsp143I GATC 5 cut(s) 255, 367, 376, 424, 1034
Bsp68I TCGCGA 1 cut(s) 193
BspACI CCGC 1 cut(s) 734
BspANI GGCC 3 cut(s) 169, 309, 324
BspCNI CTCAG 2 cut(s) 474, 590
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 2 cut(s) 7, 294
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 3 cut(s) 250, 371, 1042
BspT104I TTCGAA 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 144
BsrI ACTGG 2 cut(s) 567, 1004
BssECI CCNNGG 4 cut(s) 9, 54, 180, 1008
BssMI GATC 5 cut(s) 255, 367, 376, 424, 1034
BssNAI GTATAC 1 cut(s) 891
BssT1I CCWWGG 2 cut(s) 54, 1008
Bst1107I GTATAC 1 cut(s) 891
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 7 cut(s) 38, 218, 287, 621, 776, 996, 1067
Bst6I CTCTTC 2 cut(s) 327, 639
BstBAI YACGTR 1 cut(s) 374
BstBI TTCGAA 1 cut(s) 156
BstDEI CTNAG 4 cut(s) 482, 577, 790, 1074
BstDSI CCRYGG 1 cut(s) 9
BstEII GGTNACC 1 cut(s) 1061
BstF5I GGATG 3 cut(s) 120, 339, 454
BstFNI CGCG 1 cut(s) 193
BstHHI GCGC 1 cut(s) 801
BstKTI GATC 5 cut(s) 258, 370, 379, 427, 1037
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 5 cut(s) 255, 367, 376, 424, 1034
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNI CCWGG 1 cut(s) 786
BstNSI RCATGY 3 cut(s) 277, 944, 970
BstPI GGTNACC 1 cut(s) 1061
BstSCI CCNGG 3 cut(s) 179, 436, 784
BstSFI CTRYAG 1 cut(s) 772
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 877
BstX2I RGATCY 2 cut(s) 255, 1034
BstYI RGATCY 2 cut(s) 255, 1034
BstZ17I GTATAC 1 cut(s) 891
BsuRI GGCC 3 cut(s) 169, 309, 324
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 3 cut(s) 120, 339, 454
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 105
BtuMI TCGCGA 1 cut(s) 193
BveI ACCTGC 1 cut(s) 106
CaiI CAGNNNCTG 2 cut(s) 365, 1055
CfoI GCGC 1 cut(s) 801
Cfr13I GGNCC 2 cut(s) 293, 440
Csp6I GTAC 4 cut(s) 486, 581, 997, 1068
CviAII CATG 5 cut(s) 274, 344, 422, 941, 967
CviQI GTAC 4 cut(s) 486, 581, 997, 1068
DdeI CTNAG 4 cut(s) 482, 577, 790, 1074
DpnI GATC 5 cut(s) 257, 369, 378, 426, 1036
DpnII GATC 5 cut(s) 255, 367, 376, 424, 1034
EaeI YGGCCR 3 cut(s) 167, 307, 322
Eam1104I CTCTTC 2 cut(s) 327, 639
EarI CTCTTC 2 cut(s) 327, 639
Ecl136II GAGCTC 1 cut(s) 574
Eco130I CCWWGG 2 cut(s) 54, 1008
Eco24I GRGCYC 1 cut(s) 576
Eco47I GGWCC 2 cut(s) 293, 440
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 2 cut(s) 413, 816
Eco91I GGTNACC 1 cut(s) 1061
EcoICRI GAGCTC 1 cut(s) 574
EcoO109I RGGNCCY 1 cut(s) 293
EcoO65I GGTNACC 1 cut(s) 1061
EcoRI GAATTC 3 cut(s) 16, 416, 826
EcoRII CCWGG 1 cut(s) 784
EcoT14I CCWWGG 2 cut(s) 54, 1008
EcoT38I GRGCYC 1 cut(s) 576
ErhI CCWWGG 2 cut(s) 54, 1008
FaeI CATG 5 cut(s) 277, 347, 425, 944, 970
FaqI GGGAC 2 cut(s) 306, 1066
FatI CATG 5 cut(s) 273, 343, 421, 940, 966
FblI GTMKAC 1 cut(s) 890
Fnu4HI GCNGC 2 cut(s) 735, 866
FokI GGATG 3 cut(s) 107, 326, 461
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 2 cut(s) 735, 866
FspBI CTAG 1 cut(s) 869
GlaI GCGC 1 cut(s) 800
GluI GCNGC 2 cut(s) 735, 866
GsuI CTGGAG 2 cut(s) 584, 1021
HaeIII GGCC 3 cut(s) 169, 309, 324
HapII CCGG 2 cut(s) 180, 438
HhaI GCGC 1 cut(s) 801
Hin1II CATG 5 cut(s) 277, 347, 425, 944, 970
Hin6I GCGC 1 cut(s) 799
HinP1I GCGC 1 cut(s) 799
HinfI GANTC 4 cut(s) 61, 101, 536, 586
HpaII CCGG 2 cut(s) 180, 438
HphI GGTGA 2 cut(s) 25, 230
Hpy166II GTNNAC 1 cut(s) 891
Hpy188I TCNGA 5 cut(s) 260, 367, 432, 825, 937
Hpy188III TCNNGA 3 cut(s) 192, 380, 509
Hpy8I GTNNAC 1 cut(s) 891
HpyAV CCTTC 2 cut(s) 406, 562
HpyCH4III ACNGT 7 cut(s) 38, 218, 287, 621, 776, 996, 1067
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 8 cut(s) 115, 137, 347, 683, 701, 899, 970, 977
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 4 cut(s) 482, 577, 790, 1074
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 5 cut(s) 277, 347, 425, 944, 970
HspAI GCGC 1 cut(s) 799
Kzo9I GATC 5 cut(s) 255, 367, 376, 424, 1034
LmnI GCTCC 3 cut(s) 571, 796, 862
Lsp1109I GCAGC 1 cut(s) 877
LweI GCATC 2 cut(s) 371, 423
MaeI CTAG 1 cut(s) 869
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 1061
MalI GATC 5 cut(s) 257, 369, 378, 426, 1036
MboI GATC 5 cut(s) 255, 367, 376, 424, 1034
MboII GAAGA 7 cut(s) 344, 419, 524, 656, 822, 887, 1029
MflI RGATCY 2 cut(s) 255, 1034
MhlI GDGCHC 1 cut(s) 576
MlsI TGGCCA 1 cut(s) 169
MluCI AATT 6 cut(s) 16, 416, 475, 524, 666, 826
MluNI TGGCCA 1 cut(s) 169
MmeI TCCRAC 1 cut(s) 1012
Mox20I TGGCCA 1 cut(s) 169
MroXI GAANNNNTTC 1 cut(s) 416
MscI TGGCCA 1 cut(s) 169
MslI CAYNNNNRTG 2 cut(s) 723, 945
Msp20I TGGCCA 1 cut(s) 169
MspI CCGG 2 cut(s) 180, 438
MspR9I CCNGG 3 cut(s) 181, 438, 786
MvaI CCWGG 1 cut(s) 786
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 398
NciI CCSGG 2 cut(s) 181, 438
NdeII GATC 5 cut(s) 255, 367, 376, 424, 1034
NlaIII CATG 5 cut(s) 277, 347, 425, 944, 970
NlaIV GGNNCC 2 cut(s) 7, 294
NmeAIII GCCGAG 1 cut(s) 350
NruI TCGCGA 1 cut(s) 193
NspI RCATGY 3 cut(s) 277, 944, 970
NspV TTCGAA 1 cut(s) 156
PciI ACATGT 2 cut(s) 273, 940
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 4 cut(s) 61, 101, 536, 586
PflFI GACNNNGTC 1 cut(s) 297
PkrI GCNGC 2 cut(s) 736, 867
Ppu21I YACGTR 1 cut(s) 374
PpuMI RGGWCCY 1 cut(s) 293
PscI ACATGT 2 cut(s) 273, 940
PsiI TTATAA 1 cut(s) 690
Psp124BI GAGCTC 1 cut(s) 576
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 1 cut(s) 784
PspEI GGTNACC 1 cut(s) 1061
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 2 cut(s) 7, 294
PspPI GGNCC 2 cut(s) 293, 440
PspPPI RGGWCCY 1 cut(s) 293
PstNI CAGNNNCTG 2 cut(s) 365, 1055
PsuI RGATCY 2 cut(s) 255, 1034
PsyI GACNNNGTC 1 cut(s) 297
RruI TCGCGA 1 cut(s) 193
RsaI GTAC 4 cut(s) 487, 582, 998, 1069
RsaNI GTAC 4 cut(s) 486, 581, 997, 1068
RseI CAYNNNNRTG 2 cut(s) 723, 945
SacI GAGCTC 1 cut(s) 576
SatI GCNGC 2 cut(s) 735, 866
Sau3AI GATC 5 cut(s) 255, 367, 376, 424, 1034
Sau96I GGNCC 2 cut(s) 293, 440
ScaI AGTACT 2 cut(s) 582, 998
ScrFI CCNGG 3 cut(s) 181, 438, 786
SduI GDGCHC 1 cut(s) 576
SfaNI GCATC 2 cut(s) 371, 423
SfcI CTRYAG 1 cut(s) 772
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 2 cut(s) 293, 440
SmiMI CAYNNNNRTG 2 cut(s) 723, 945
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 6 cut(s) 16, 416, 475, 524, 666, 826
SsiI CCGC 1 cut(s) 734
SspMI CTAG 1 cut(s) 869
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 3 cut(s) 179, 436, 784
StyI CCWWGG 2 cut(s) 54, 1008
TaaI ACNGT 7 cut(s) 38, 218, 287, 621, 776, 996, 1067
TaiI ACGT 1 cut(s) 376
TaqI TCGA 1 cut(s) 156
TasI AATT 6 cut(s) 16, 416, 475, 524, 666, 826
TatI WGTACW 3 cut(s) 485, 580, 996
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 4 cut(s) 61, 101, 536, 586
TscAI CASTG 1 cut(s) 112
TseI GCWGC 1 cut(s) 865
TspDTI ATGAA 3 cut(s) 328, 425, 537
TspGWI ACGGA 1 cut(s) 364
TspRI CASTG 1 cut(s) 112
Tth111I GACNNNGTC 1 cut(s) 297
VpaK11BI GGWCC 2 cut(s) 293, 440
XapI RAATTY 4 cut(s) 16, 416, 524, 826
XceI RCATGY 3 cut(s) 277, 944, 970
XmiI GTMKAC 1 cut(s) 890
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 1 cut(s) 869
ZrmI AGTACT 2 cut(s) 582, 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.