MD00G1152700.v1.1

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002182
Physical Location & Seq
Reverse (-)
33430318 .. 33436041
5724 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1152700.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGAAAATGTCAACAACAGAGAAGATCGTATTCCAGCAGAAGCTAGGGAGATTCTACAATCAATGGCTTCCCAGTGGGAAGATGTGGTAGATTCAAAGGCACTGCAGGTGATCCCTCTCAAGGGTGCAATGACTAATGAGGTTTTCCAAATAAAGTGGCCATCAAGGACAGGGGAACTGTCACATAAAGTTCTGGTTAGGATTTATGGTGAGGGTGTCGAGGTGTTCTTTGACAGGGATAATGAGATCCGGACATTTGAACACATGTCAAAGAATGGTCAGGGACCTCGTCTGCTTGGGCGTTTTCCAAATGGCCGAATTGAAGAGTTCATCCATGCACGGACACTCTCAGCATGTGATCTACGTGATCCGGATATATCTGCTCTTATAGCTTCTAAATTGAAGGAGTTCCACGAACTTGATATGCCTGGTCCAAAGGTTGTCACCCTCTGGGATAGATTGCGAAATTGGCTCAGTACGGCCAAGAGATTGTCTACACCAGAAGAAGCTAACTCCTTTCAGTTAGATTCCATTGAAAAGGAAATCCCGTTACTGGAGAAAGAGCTCTCAGGGCCTCATCAACGCATTGGTTTTTGCCACAATGATTTACAGTATGGTAATATAATGCTTGAGGAAGAGAGTAAATCGATAACCGTAATCGACTATGAATATGCAAGTTATAATGCTGTAGCATTTGACATAGCAAATCATTTCTGTGAGATGGCAGCTGACTATCACACTGATACACCTCATATTTTGGACTACAGCAAATATCCTGGTTCAGAGGAGCGTCAGCGGTTTGTTTGTTTGTATCTGAGTTTTTCTGGCAATCAACCTACTGAGAGCGAAGTGGAGCAGCTGGTTCAAGATGTTGAGAACTATACCCTTGCAAGCCATCTCTTTTGGGGCTTATGGGGAATAATTTCGGAACATGTGAATGAAATTGACTTTGACTACATGGAATACGCAAGGCAGAGGTTTCACCAGTACTGGATAAGCAGGCCTAAGCTGTTGGGTTCTGTTGGATCTCCTCCAAATCTTGTAACTGATGTTAAATATGGTATGCATCGAGCTGATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

361

Amino Acids

41.59

Weight (kDa)

5.08

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_kinase PF01633 38 - 331 1.6e-101 Choline/ethanolamine kinase
APH PF01636 39 - 238 6.4e-11 Phosphotransferase enzyme family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 681
AarI CACCTGC 1 cut(s) 97
Acc36I ACCTGC 1 cut(s) 97
AccI GTMKAC 1 cut(s) 494
AccIII TCCGGA 2 cut(s) 249, 370
AciI CCGC 1 cut(s) 796
AclWI GGATC 4 cut(s) 106, 241, 362, 1033
AcoI YGGCCR 3 cut(s) 158, 313, 480
AfaI GTAC 2 cut(s) 478, 989
AfiI CCNNNNNNNGG 3 cut(s) 121, 122, 553
AflIII ACRYGT 2 cut(s) 264, 931
AgsI TTSAA 6 cut(s) 96, 260, 323, 403, 536, 866
AjnI CCWGG 2 cut(s) 427, 775
AluBI AGCT 8 cut(s) 44, 392, 509, 565, 728, 859, 1009, 1073
AluI AGCT 8 cut(s) 44, 392, 509, 565, 728, 859, 1009, 1073
Alw21I GWGCWC 1 cut(s) 567
AlwI GGATC 4 cut(s) 106, 241, 362, 1033
Aor13HI TCCGGA 2 cut(s) 249, 370
AoxI GGCC 5 cut(s) 158, 313, 480, 572, 1001
ApeKI GCWGC 2 cut(s) 725, 856
Asp700I GAANNNNTTC 2 cut(s) 407, 922
AspS9I GGNCC 3 cut(s) 284, 431, 572
AsuHPI GGTGA 4 cut(s) 121, 221, 436, 974
AvaII GGWCC 2 cut(s) 284, 431
BalI TGGCCA 1 cut(s) 160
BanII GRGCYC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 567
BbvI GCAGC 2 cut(s) 737, 868
BccI CCATC 3 cut(s) 169, 715, 903
BceAI ACGGC 1 cut(s) 495
BciT130I CCWGG 2 cut(s) 429, 777
BfaI CTAG 1 cut(s) 45
BfmI CTRYAG 3 cut(s) 104, 687, 763
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 2 cut(s) 726, 857
BlsI GCNGC 2 cut(s) 727, 858
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 2 cut(s) 429, 777
Bme18I GGWCC 2 cut(s) 284, 431
BmgT120I GGNCC 3 cut(s) 284, 431, 572
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 2 cut(s) 429, 777
BmrI ACTGGG 1 cut(s) 67
BmsI GCATC 1 cut(s) 1075
BmuI ACTGGG 1 cut(s) 67
BpmI CTGGAG 1 cut(s) 575
Bpu10I CCTNAGC 1 cut(s) 1005
BpuEI CTTGAG 2 cut(s) 104, 650
Bsa29I ATCGAT 1 cut(s) 647
BsaAI YACGTR 1 cut(s) 365
BsaWI WCCGGW 2 cut(s) 249, 370
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 122, 553
Bse1I ACTGG 4 cut(s) 73, 558, 985, 995
Bse3DI GCAATG 1 cut(s) 135
BseAI TCCGGA 2 cut(s) 249, 370
BseBI CCWGG 2 cut(s) 429, 777
BseCI ATCGAT 1 cut(s) 647
BseGI GGATG 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 121, 122, 553
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 5 cut(s) 363, 487, 582, 806, 831
BseNI ACTGG 4 cut(s) 73, 558, 985, 995
BseRI GAGGAG 2 cut(s) 800, 1020
BseXI GCAGC 2 cut(s) 737, 868
BshFI GGCC 5 cut(s) 160, 315, 482, 574, 1003
BshVI ATCGAT 1 cut(s) 647
BsiHKAI GWGCWC 1 cut(s) 567
BsiSI CCGG 2 cut(s) 250, 371
BslFI GGGAC 1 cut(s) 297
BslI CCNNNNNNNGG 3 cut(s) 121, 122, 553
BsmFI GGGAC 1 cut(s) 297
BsnI GGCC 5 cut(s) 160, 315, 482, 574, 1003
Bsp1286I GDGCHC 1 cut(s) 567
Bsp13I TCCGGA 2 cut(s) 249, 370
Bsp143I GATC 6 cut(s) 25, 111, 246, 358, 367, 1025
BspACI CCGC 1 cut(s) 796
BspANI GGCC 5 cut(s) 160, 315, 482, 574, 1003
BspCNI CTCAG 5 cut(s) 362, 486, 581, 807, 832
BspDI ATCGAT 1 cut(s) 647
BspEI TCCGGA 2 cut(s) 249, 370
BspLI GGNNCC 1 cut(s) 285
BspMAI CTGCAG 1 cut(s) 108
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 4 cut(s) 106, 241, 362, 1033
BsrDI GCAATG 1 cut(s) 135
BsrI ACTGG 4 cut(s) 73, 558, 985, 995
BssMI GATC 6 cut(s) 25, 111, 246, 358, 367, 1025
Bst2UI CCWGG 2 cut(s) 429, 777
Bst4CI ACNGT 3 cut(s) 180, 612, 655
Bst6I CTCTTC 2 cut(s) 318, 630
BstBAI YACGTR 1 cut(s) 365
BstC8I GCNNGC 2 cut(s) 892, 1001
BstDEI CTNAG 6 cut(s) 349, 473, 568, 815, 840, 1005
BstF5I GGATG 1 cut(s) 330
BstKTI GATC 6 cut(s) 28, 114, 249, 361, 370, 1028
BstMBI GATC 6 cut(s) 25, 111, 246, 358, 367, 1025
BstMWI GCNNNNNNNGC 3 cut(s) 389, 469, 571
BstNI CCWGG 2 cut(s) 429, 777
BstNSI RCATGY 3 cut(s) 268, 357, 935
BstSCI CCNGG 2 cut(s) 427, 775
BstSFI CTRYAG 3 cut(s) 104, 687, 763
BstV1I GCAGC 2 cut(s) 737, 868
BstX2I RGATCY 2 cut(s) 246, 1025
BstYI RGATCY 2 cut(s) 246, 1025
Bsu15I ATCGAT 1 cut(s) 647
BsuRI GGCC 5 cut(s) 160, 315, 482, 574, 1003
BsuTUI ATCGAT 1 cut(s) 647
BtsCI GGATG 1 cut(s) 330
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 3 cut(s) 80, 101, 738
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 2 cut(s) 892, 1001
Cfr13I GGNCC 3 cut(s) 284, 431, 572
ClaI ATCGAT 1 cut(s) 647
CseI GACGC 1 cut(s) 779
Csp6I GTAC 2 cut(s) 477, 988
CspCI CAANNNNNGTGG 2 cut(s) 137, 172
CviAII CATG 5 cut(s) 265, 335, 354, 932, 958
CviQI GTAC 2 cut(s) 477, 988
DdeI CTNAG 6 cut(s) 349, 473, 568, 815, 840, 1005
DpnI GATC 6 cut(s) 27, 113, 248, 360, 369, 1027
DpnII GATC 6 cut(s) 25, 111, 246, 358, 367, 1025
EaeI YGGCCR 3 cut(s) 158, 313, 480
Eam1104I CTCTTC 2 cut(s) 318, 630
EarI CTCTTC 2 cut(s) 318, 630
Ecl136II GAGCTC 1 cut(s) 565
Eco147I AGGCCT 1 cut(s) 1003
Eco24I GRGCYC 1 cut(s) 567
Eco47I GGWCC 2 cut(s) 284, 431
Eco53kI GAGCTC 1 cut(s) 565
EcoICRI GAGCTC 1 cut(s) 565
EcoO109I RGGNCCY 2 cut(s) 284, 572
EcoRII CCWGG 2 cut(s) 427, 775
EcoT22I ATGCAT 1 cut(s) 1068
EcoT38I GRGCYC 1 cut(s) 567
FaeI CATG 5 cut(s) 268, 338, 357, 935, 961
FaqI GGGAC 1 cut(s) 297
FatI CATG 5 cut(s) 264, 334, 353, 931, 957
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 2 cut(s) 726, 857
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 2 cut(s) 726, 857
FspBI CTAG 1 cut(s) 45
GluI GCNGC 2 cut(s) 726, 857
GsuI CTGGAG 1 cut(s) 575
HaeIII GGCC 5 cut(s) 160, 315, 482, 574, 1003
HapII CCGG 2 cut(s) 250, 371
HgaI GACGC 1 cut(s) 779
Hin1II CATG 5 cut(s) 268, 338, 357, 935, 961
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 3 cut(s) 52, 92, 527
HpaII CCGG 2 cut(s) 250, 371
HphI GGTGA 4 cut(s) 121, 221, 436, 974
Hpy166II GTNNAC 2 cut(s) 13, 495
Hpy188I TCNGA 3 cut(s) 784, 816, 928
Hpy188III TCNNGA 3 cut(s) 250, 371, 866
Hpy8I GTNNAC 2 cut(s) 13, 495
HpyAV CCTTC 1 cut(s) 397
HpyCH4III ACNGT 3 cut(s) 180, 612, 655
HpyCH4IV ACGT 1 cut(s) 364
HpyCH4V TGCA 6 cut(s) 106, 128, 338, 674, 890, 1066
HpyF10VI GCNNNNNNNGC 3 cut(s) 389, 469, 571
HpyF3I CTNAG 6 cut(s) 349, 473, 568, 815, 840, 1005
HpySE526I ACGT 1 cut(s) 364
Hsp92II CATG 5 cut(s) 268, 338, 357, 935, 961
Kpn2I TCCGGA 2 cut(s) 249, 370
Kzo9I GATC 6 cut(s) 25, 111, 246, 358, 367, 1025
LmnI GCTCC 2 cut(s) 787, 853
Lsp1109I GCAGC 2 cut(s) 737, 868
LweI GCATC 1 cut(s) 1075
MaeI CTAG 1 cut(s) 45
MaeII ACGT 1 cut(s) 364
MaeIII GTNAC 4 cut(s) 180, 442, 549, 1042
MalI GATC 6 cut(s) 27, 113, 248, 360, 369, 1027
MboI GATC 6 cut(s) 25, 111, 246, 358, 367, 1025
MboII GAAGA 5 cut(s) 35, 92, 335, 515, 647
MflI RGATCY 2 cut(s) 246, 1025
MhlI GDGCHC 1 cut(s) 567
MlsI TGGCCA 1 cut(s) 160
MluCI AATT 5 cut(s) 318, 398, 466, 921, 942
MluNI TGGCCA 1 cut(s) 160
MmeI TCCRAC 1 cut(s) 1003
Mox20I TGGCCA 1 cut(s) 160
Mph1103I ATGCAT 1 cut(s) 1068
MroI TCCGGA 2 cut(s) 249, 370
MroXI GAANNNNTTC 2 cut(s) 407, 922
MscI TGGCCA 1 cut(s) 160
MseI TTAA 2 cut(s) 1053, 1081
MslI CAYNNNNRTG 2 cut(s) 714, 936
Msp20I TGGCCA 1 cut(s) 160
MspA1I CMGCKG 3 cut(s) 728, 796, 859
MspI CCGG 2 cut(s) 250, 371
MspR9I CCNGG 2 cut(s) 429, 777
MvaI CCWGG 2 cut(s) 429, 777
MwoI GCNNNNNNNGC 3 cut(s) 389, 469, 571
NdeII GATC 6 cut(s) 25, 111, 246, 358, 367, 1025
NlaIII CATG 5 cut(s) 268, 338, 357, 935, 961
NlaIV GGNNCC 1 cut(s) 285
NmuCI GTSAC 2 cut(s) 180, 442
NsiI ATGCAT 1 cut(s) 1068
NspI RCATGY 3 cut(s) 268, 357, 935
PaqCI CACCTGC 1 cut(s) 97
PceI AGGCCT 1 cut(s) 1003
PciI ACATGT 2 cut(s) 264, 931
PdmI GAANNNNTTC 2 cut(s) 407, 922
PfeI GAWTC 3 cut(s) 52, 92, 527
PflFI GACNNNGTC 1 cut(s) 288
PkrI GCNGC 2 cut(s) 727, 858
Ppu21I YACGTR 1 cut(s) 365
PpuMI RGGWCCY 1 cut(s) 284
PscI ACATGT 2 cut(s) 264, 931
PsiI TTATAA 1 cut(s) 681
Psp124BI GAGCTC 1 cut(s) 567
Psp5II RGGWCCY 1 cut(s) 284
Psp6I CCWGG 2 cut(s) 427, 775
PspGI CCWGG 2 cut(s) 427, 775
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 3 cut(s) 284, 431, 572
PspPPI RGGWCCY 1 cut(s) 284
PstI CTGCAG 1 cut(s) 108
PsuI RGATCY 2 cut(s) 246, 1025
PsyI GACNNNGTC 1 cut(s) 288
PvuII CAGCTG 2 cut(s) 728, 859
RsaI GTAC 2 cut(s) 478, 989
RsaNI GTAC 2 cut(s) 477, 988
RseI CAYNNNNRTG 2 cut(s) 714, 936
SacI GAGCTC 1 cut(s) 567
SaqAI TTAA 2 cut(s) 1053, 1081
SatI GCNGC 2 cut(s) 726, 857
Sau3AI GATC 6 cut(s) 25, 111, 246, 358, 367, 1025
Sau96I GGNCC 3 cut(s) 284, 431, 572
ScaI AGTACT 1 cut(s) 989
ScrFI CCNGG 2 cut(s) 429, 777
SduI GDGCHC 1 cut(s) 567
SfaNI GCATC 1 cut(s) 1075
SfcI CTRYAG 3 cut(s) 104, 687, 763
SinI GGWCC 2 cut(s) 284, 431
SmiMI CAYNNNNRTG 2 cut(s) 714, 936
SmlI CTYRAG 2 cut(s) 119, 629
SmoI CTYRAG 2 cut(s) 119, 629
Sse9I AATT 5 cut(s) 318, 398, 466, 921, 942
SseBI AGGCCT 1 cut(s) 1003
SsiI CCGC 1 cut(s) 796
SspMI CTAG 1 cut(s) 45
SstI GAGCTC 1 cut(s) 567
StuI AGGCCT 1 cut(s) 1003
StyD4I CCNGG 2 cut(s) 427, 775
TaaI ACNGT 3 cut(s) 180, 612, 655
TaiI ACGT 1 cut(s) 367
TaqI TCGA 4 cut(s) 219, 647, 660, 1069
TasI AATT 5 cut(s) 318, 398, 466, 921, 942
TatI WGTACW 1 cut(s) 987
TfiI GAWTC 3 cut(s) 52, 92, 527
Tru1I TTAA 2 cut(s) 1053, 1081
Tru9I TTAA 2 cut(s) 1053, 1081
TscAI CASTG 3 cut(s) 80, 108, 745
TseFI GTSAC 2 cut(s) 180, 442
TseI GCWGC 2 cut(s) 725, 856
Tsp45I GTSAC 2 cut(s) 180, 442
TspDTI ATGAA 3 cut(s) 319, 681, 954
TspGWI ACGGA 1 cut(s) 355
TspRI CASTG 3 cut(s) 80, 108, 745
Tth111I GACNNNGTC 1 cut(s) 288
VpaK11BI GGWCC 2 cut(s) 284, 431
XceI RCATGY 3 cut(s) 268, 357, 935
XmiI GTMKAC 1 cut(s) 494
XmnI GAANNNNTTC 2 cut(s) 407, 922
XspI CTAG 1 cut(s) 45
ZrmI AGTACT 1 cut(s) 989
Zsp2I ATGCAT 1 cut(s) 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.