Rw1G006740

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002182
Physical Location & Seq
Reverse (-)
13885807 .. 13890189
4383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G006740.1

Sequence Viewer

Length: 795 bp
ATGCGTGAAGTACTTTTATTCCCTTCCAGAAAAATGTGTTACATCTTTCTTTTCACTTGTGAATTTCAGACGCTTTCAGCATCTGATCTACGTGATCCAGATATATCTGCTCTTATAGCTACTAAAATGAAGGAATTCCATGATCTTGAGATGCCCGGTCCAAAGGATGTCGTCCTTTGGGGCAGATTGAGAAATTGGCTGAGTACAGCCAAGAGATTGTGTAGTCCAGAAGAAGCTAATGAATTTCAGTTAGATTCCATTGAAAAGGAAATCTCTATACTGGAGAAGGAGCTCTCAGTACTGAATCAGCAGTTAGGTTTCTGCCACAATGATTTACAGTATGGAAACATAATGATTGAGGAAGAGAGTAAAACGATAACGATAATTGACTATGAGTATGCAAGTTATAATCCTGTTGCATTTGACATAGCAAACCATTTCTGTGAGATGGCGGCTGACTATCACACAGATACCCCTCATATTTTAGACTACAGTAAATATCCTGGCTTAGAGGAGCGCAAGAGGTTTGTTCGTATGTATCTGAGTTCTTCAGGCAATCAACCTAGTGACAAAGAAGTGGAGCAGCTAGTTGAAGATGTTGAGAAGTATACCCTTGCAGACCATCTGTTCTGGGGCTTATGGGGAATAATATCGGAACATGTGAATGATATTGATTTTGACTACATGCAATATGCAAAGCAGAGGTTTCAACAGTACTGGAGAACCAAGGCTTTGCTATTGGGTTCTGTTGGATCTTCCCCAGACACAGGGACTGATGGTAACCGTACTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

264

Amino Acids

30.69

Weight (kDa)

4.79

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_kinase PF01633 23 - 240 1.1e-72 Choline/ethanolamine kinase
APH PF01636 64 - 147 3.8e-10 Phosphotransferase enzyme family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 408
AccI GTMKAC 1 cut(s) 608
AciI CCGC 1 cut(s) 452
AclWI GGATC 2 cut(s) 89, 760
AcsI RAATTY 3 cut(s) 62, 134, 242
AcuI CTGAAG 1 cut(s) 534
AfaI GTAC 5 cut(s) 12, 205, 300, 716, 787
AfiI CCNNNNNNNGG 1 cut(s) 767
AflIII ACRYGT 1 cut(s) 658
AgsI TTSAA 3 cut(s) 263, 593, 710
AjnI CCWGG 1 cut(s) 502
AluBI AGCT 4 cut(s) 119, 236, 292, 586
AluI AGCT 4 cut(s) 119, 236, 292, 586
Alw21I GWGCWC 1 cut(s) 294
AlwI GGATC 2 cut(s) 89, 760
AlwNI CAGNNNCTG 2 cut(s) 83, 773
ApeKI GCWGC 1 cut(s) 583
ApoI RAATTY 3 cut(s) 62, 134, 242
Asp700I GAANNNNTTC 1 cut(s) 134
AspLEI GCGC 1 cut(s) 519
AspS9I GGNCC 1 cut(s) 158
AsuC2I CCSGG 1 cut(s) 156
AvaII GGWCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 595
BccI CCATC 3 cut(s) 442, 630, 770
BciT130I CCWGG 1 cut(s) 504
BcnI CCSGG 1 cut(s) 156
BfaI CTAG 2 cut(s) 564, 587
BfmI CTRYAG 1 cut(s) 490
BisI GCNGC 2 cut(s) 453, 584
BlsI GCNGC 2 cut(s) 454, 585
BmcAI AGTACT 3 cut(s) 12, 300, 716
Bme1390I CCNGG 2 cut(s) 156, 504
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmrFI CCNGG 2 cut(s) 156, 504
BmsI GCATC 2 cut(s) 89, 141
BpmI CTGGAG 2 cut(s) 302, 739
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 726
Bsc4I CCNNNNNNNGG 1 cut(s) 767
Bse1I ACTGG 2 cut(s) 285, 722
BseBI CCWGG 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 726
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 767
BseMII CTCAG 3 cut(s) 191, 309, 533
BseNI ACTGG 2 cut(s) 285, 722
BseRI GAGGAG 1 cut(s) 527
BseXI GCAGC 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 294
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 784
BslI CCNNNNNNNGG 1 cut(s) 767
BsmFI GGGAC 1 cut(s) 784
Bsp1286I GDGCHC 1 cut(s) 294
Bsp143I GATC 4 cut(s) 85, 94, 142, 752
BspACI CCGC 1 cut(s) 452
BspCNI CTCAG 3 cut(s) 192, 308, 534
BspPI GGATC 2 cut(s) 89, 760
BsrI ACTGG 2 cut(s) 285, 722
BssECI CCNNGG 1 cut(s) 726
BssMI GATC 4 cut(s) 85, 94, 142, 752
BssNAI GTATAC 1 cut(s) 609
BssT1I CCWWGG 1 cut(s) 726
Bst1107I GTATAC 1 cut(s) 609
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 4 cut(s) 339, 494, 714, 785
Bst6I CTCTTC 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 92
BstDEI CTNAG 5 cut(s) 200, 295, 508, 542, 792
BstEII GGTNACC 1 cut(s) 779
BstF5I GGATG 1 cut(s) 172
BstHHI GCGC 1 cut(s) 519
BstKTI GATC 4 cut(s) 88, 97, 145, 755
BstMBI GATC 4 cut(s) 85, 94, 142, 752
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 1 cut(s) 504
BstNSI RCATGY 2 cut(s) 662, 688
BstPI GGTNACC 1 cut(s) 779
BstSCI CCNGG 2 cut(s) 154, 502
BstSFI CTRYAG 1 cut(s) 490
BstV1I GCAGC 1 cut(s) 595
BstX2I RGATCY 1 cut(s) 752
BstYI RGATCY 1 cut(s) 752
BstZ17I GTATAC 1 cut(s) 609
BtsCI GGATG 1 cut(s) 172
CaiI CAGNNNCTG 2 cut(s) 83, 773
CfoI GCGC 1 cut(s) 519
Cfr13I GGNCC 1 cut(s) 158
CseI GACGC 1 cut(s) 79
Csp6I GTAC 5 cut(s) 11, 204, 299, 715, 786
CviAII CATG 3 cut(s) 140, 659, 685
CviQI GTAC 5 cut(s) 11, 204, 299, 715, 786
DdeI CTNAG 5 cut(s) 200, 295, 508, 542, 792
DpnI GATC 4 cut(s) 87, 96, 144, 754
DpnII GATC 4 cut(s) 85, 94, 142, 752
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 292
Eco130I CCWWGG 1 cut(s) 726
Eco24I GRGCYC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 158
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 534
Eco91I GGTNACC 1 cut(s) 779
EcoICRI GAGCTC 1 cut(s) 292
EcoO65I GGTNACC 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 502
EcoT14I CCWWGG 1 cut(s) 726
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 726
FaeI CATG 3 cut(s) 143, 662, 688
FaqI GGGAC 1 cut(s) 784
FatI CATG 3 cut(s) 139, 658, 684
FblI GTMKAC 1 cut(s) 608
Fnu4HI GCNGC 2 cut(s) 453, 584
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 2 cut(s) 453, 584
FspBI CTAG 2 cut(s) 564, 587
GlaI GCGC 1 cut(s) 518
GluI GCNGC 2 cut(s) 453, 584
GsuI CTGGAG 2 cut(s) 302, 739
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 79
HhaI GCGC 1 cut(s) 519
Hin1II CATG 3 cut(s) 143, 662, 688
Hin6I GCGC 1 cut(s) 517
HinP1I GCGC 1 cut(s) 517
HinfI GANTC 2 cut(s) 254, 304
HpaII CCGG 1 cut(s) 156
Hpy166II GTNNAC 1 cut(s) 609
Hpy188I TCNGA 4 cut(s) 69, 85, 543, 655
Hpy188III TCNNGA 4 cut(s) 27, 98, 146, 227
Hpy8I GTNNAC 1 cut(s) 609
HpyAV CCTTC 3 cut(s) 33, 124, 280
HpyCH4III ACNGT 4 cut(s) 339, 494, 714, 785
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 5 cut(s) 401, 419, 617, 688, 695
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 5 cut(s) 200, 295, 508, 542, 792
HpySE526I ACGT 1 cut(s) 91
Hsp92II CATG 3 cut(s) 143, 662, 688
HspAI GCGC 1 cut(s) 517
Kzo9I GATC 4 cut(s) 85, 94, 142, 752
LmnI GCTCC 3 cut(s) 289, 514, 580
Lsp1109I GCAGC 1 cut(s) 595
LweI GCATC 2 cut(s) 89, 141
MaeI CTAG 2 cut(s) 564, 587
MaeII ACGT 1 cut(s) 91
MaeIII GTNAC 3 cut(s) 38, 566, 779
MalI GATC 4 cut(s) 87, 96, 144, 754
MboI GATC 4 cut(s) 85, 94, 142, 752
MboII GAAGA 5 cut(s) 242, 374, 540, 605, 747
MflI RGATCY 1 cut(s) 752
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 5 cut(s) 62, 134, 193, 242, 384
MmeI TCCRAC 1 cut(s) 730
MnlI CCTC 5 cut(s) 352, 486, 505, 516, 696
MroXI GAANNNNTTC 1 cut(s) 134
MslI CAYNNNNRTG 2 cut(s) 441, 663
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 156, 504
MvaI CCWGG 1 cut(s) 504
MwoI GCNNNNNNNGC 1 cut(s) 116
NciI CCSGG 1 cut(s) 156
NdeII GATC 4 cut(s) 85, 94, 142, 752
NlaIII CATG 3 cut(s) 143, 662, 688
NmuCI GTSAC 1 cut(s) 566
NspI RCATGY 2 cut(s) 662, 688
PciI ACATGT 1 cut(s) 658
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 2 cut(s) 254, 304
PkrI GCNGC 2 cut(s) 454, 585
Ppu21I YACGTR 1 cut(s) 92
PscI ACATGT 1 cut(s) 658
PsiI TTATAA 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 502
PspEI GGTNACC 1 cut(s) 779
PspGI CCWGG 1 cut(s) 502
PspPI GGNCC 1 cut(s) 158
PstNI CAGNNNCTG 2 cut(s) 83, 773
PsuI RGATCY 1 cut(s) 752
RsaI GTAC 5 cut(s) 12, 205, 300, 716, 787
RsaNI GTAC 5 cut(s) 11, 204, 299, 715, 786
RseI CAYNNNNRTG 2 cut(s) 441, 663
SacI GAGCTC 1 cut(s) 294
SatI GCNGC 2 cut(s) 453, 584
Sau3AI GATC 4 cut(s) 85, 94, 142, 752
Sau96I GGNCC 1 cut(s) 158
ScaI AGTACT 3 cut(s) 12, 300, 716
ScrFI CCNGG 2 cut(s) 156, 504
SduI GDGCHC 1 cut(s) 294
SetI ASST 9 cut(s) 94, 121, 238, 294, 319, 527, 565, 588, 707
SfaNI GCATC 2 cut(s) 89, 141
SfcI CTRYAG 1 cut(s) 490
SinI GGWCC 1 cut(s) 158
SmiMI CAYNNNNRTG 2 cut(s) 441, 663
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 5 cut(s) 62, 134, 193, 242, 384
SsiI CCGC 1 cut(s) 452
SspMI CTAG 2 cut(s) 564, 587
SstI GAGCTC 1 cut(s) 294
StyD4I CCNGG 2 cut(s) 154, 502
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 4 cut(s) 339, 494, 714, 785
TaiI ACGT 1 cut(s) 94
TasI AATT 5 cut(s) 62, 134, 193, 242, 384
TatI WGTACW 4 cut(s) 10, 203, 298, 714
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 2 cut(s) 254, 304
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 1 cut(s) 583
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 2 cut(s) 143, 255
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 3 cut(s) 62, 134, 242
XceI RCATGY 2 cut(s) 662, 688
XmiI GTMKAC 1 cut(s) 608
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 2 cut(s) 564, 587
ZrmI AGTACT 3 cut(s) 12, 300, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.