Rw3G022890

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002182
Physical Location & Seq
Forward (+)
28342195 .. 28347810
5616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022890.1

Sequence Viewer

Length: 903 bp
ATGGGTGCCGTGGTGAATTCCATAGATAAAGACGACCGTATTCCAAAAGAAGCCAAGGAGATTCTACAATCATTGGCTACCAAGTGGGAAAATGTAGCTGATTCCACTGCCTTGCAGGTCATCCCTCTCAAGGGTGCAATGACTAACGAGATGTTCGAAATAAAGTGGCCAACAAGCACCGGGGAAGTCTCGCGAAAGGTTGTGGTTAGGATTTACAGTGAGGGTGTTGAGGTGTTCTTTGACAGGGATAATGAGATCCGAACATTTGAATACATGTCAAAGAACGGTCAGGGACCTCGTCTGCTTGGCCGTTTTCCTAATGGCCGAGTTGAAGAGTTCATCCATGCACGGACACTTTCAGCATCTGATCTACGTGATCCAGATATATCTGCTCTTATAGCTACTAAAATGAAGGAATTCCATGATCTTGAGATGCCCGGTCCAAGGGATGTCGTCCTCTGGGGCAGATTGAGTTTGATATATGTGGCCATCGTAAACATGCTAGAGAAGAAGCTGGAGACTCTGAAGAACAAGGAATCTGGGAATTTTATAGTAATACCTAAAACTGGACTGATGTCTCATGATTATGAACATATTGAAATGTCACCCAAAAATCGATGTACAGAAAACCAATGCACATCGATATCTAAACACCAAATCGTTATCTACTGTCAACGTACAAATCATGTCGTTAATGACCAGTCGACAGGGGGAGTTGACCTATTAGTCAAAGAAATCTTACAGTCGACTAATCAAGCAATCGACCTATCAAAAAAAAGGGGACTGAATATCGAGGTATGGCACATGGAGTGTGTGAACTCCTATGGTTACGTAATCTTCTCTGAGATTTGGGTTTTAAGCCTAAGTCAGCTATGCAGCTGTATTGTGACAACAAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

300

Amino Acids

34.05

Weight (kDa)

6.2

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_kinase PF01633 41 - 178 2.6e-36 Choline/ethanolamine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 725
Acc36I ACCTGC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 5
AccI GTMKAC 2 cut(s) 704, 746
AccII CGCG 1 cut(s) 193
AclWI GGATC 2 cut(s) 250, 371
AcoI YGGCCR 4 cut(s) 167, 307, 322, 486
AcsI RAATTY 3 cut(s) 16, 416, 544
AcuI CTGAAG 1 cut(s) 545
AfaI GTAC 2 cut(s) 622, 679
AfiI CCNNNNNNNGG 4 cut(s) 130, 131, 444, 566
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 3 cut(s) 269, 332, 599
AluBI AGCT 6 cut(s) 98, 401, 514, 871, 879, 898
AluI AGCT 6 cut(s) 98, 401, 514, 871, 879, 898
Alw26I GTCTC 3 cut(s) 193, 512, 582
AlwI GGATC 2 cut(s) 250, 371
AlwNI CAGNNNCTG 1 cut(s) 365
AoxI GGCC 4 cut(s) 167, 307, 322, 486
ApeKI GCWGC 1 cut(s) 876
ApoI RAATTY 3 cut(s) 16, 416, 544
Asp700I GAANNNNTTC 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 293, 440
AsuC2I CCSGG 2 cut(s) 181, 438
AsuHPI GGTGA 2 cut(s) 25, 597
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 2 cut(s) 293, 440
BalI TGGCCA 2 cut(s) 169, 488
BanI GGYRCC 1 cut(s) 5
BbvI GCAGC 1 cut(s) 888
BccI CCATC 1 cut(s) 497
BceAI ACGGC 1 cut(s) 294
BcnI CCSGG 2 cut(s) 181, 438
BcoDI GTCTC 3 cut(s) 193, 512, 582
BfaI CTAG 1 cut(s) 503
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 1 cut(s) 877
BlsI GCNGC 1 cut(s) 878
Bme1390I CCNGG 2 cut(s) 181, 438
Bme18I GGWCC 2 cut(s) 293, 440
BmgT120I GGNCC 2 cut(s) 293, 440
BmiI GGNNCC 2 cut(s) 7, 294
BmrFI CCNGG 2 cut(s) 181, 438
BmsI GCATC 2 cut(s) 371, 423
BoxI GACNNNNGTC 1 cut(s) 574
BpmI CTGGAG 1 cut(s) 536
Bpu14I TTCGAA 1 cut(s) 156
BpuEI CTTGAG 2 cut(s) 113, 449
BpuMI CCSGG 2 cut(s) 181, 438
Bsa29I ATCGAT 2 cut(s) 616, 641
BsaAI YACGTR 2 cut(s) 374, 832
BsaJI CCNNGG 4 cut(s) 9, 54, 180, 443
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 131, 444, 566
Bse1I ACTGG 2 cut(s) 571, 700
Bse3DI GCAATG 1 cut(s) 144
BseCI ATCGAT 2 cut(s) 616, 641
BseDI CCNNGG 4 cut(s) 9, 54, 180, 443
BseGI GGATG 3 cut(s) 120, 339, 454
BseLI CCNNNNNNNGG 4 cut(s) 130, 131, 444, 566
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 2 cut(s) 571, 700
BseXI GCAGC 1 cut(s) 888
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 4 cut(s) 169, 309, 324, 488
BshNI GGYRCC 1 cut(s) 5
BshVI ATCGAT 2 cut(s) 616, 641
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 2 cut(s) 180, 438
BslFI GGGAC 2 cut(s) 306, 795
BslI CCNNNNNNNGG 4 cut(s) 130, 131, 444, 566
BsmAI GTCTC 3 cut(s) 193, 512, 582
BsmFI GGGAC 2 cut(s) 306, 795
BsnI GGCC 4 cut(s) 169, 309, 324, 488
Bsp119I TTCGAA 1 cut(s) 156
Bsp1407I TGTACA 1 cut(s) 620
Bsp143I GATC 4 cut(s) 255, 367, 376, 424
Bsp68I TCGCGA 1 cut(s) 193
BspANI GGCC 4 cut(s) 169, 309, 324, 488
BspCNI CTCAG 1 cut(s) 835
BspDI ATCGAT 2 cut(s) 616, 641
BspFNI CGCG 1 cut(s) 193
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 2 cut(s) 7, 294
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 2 cut(s) 250, 371
BspT104I TTCGAA 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 144
BsrGI TGTACA 1 cut(s) 620
BsrI ACTGG 2 cut(s) 571, 700
BssECI CCNNGG 4 cut(s) 9, 54, 180, 443
BssMI GATC 4 cut(s) 255, 367, 376, 424
BssT1I CCWWGG 2 cut(s) 54, 443
Bst4CI ACNGT 5 cut(s) 38, 218, 287, 671, 744
Bst6I CTCTTC 1 cut(s) 327
BstAUI TGTACA 1 cut(s) 620
BstBAI YACGTR 2 cut(s) 374, 832
BstBI TTCGAA 1 cut(s) 156
BstDEI CTNAG 2 cut(s) 843, 863
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 3 cut(s) 120, 339, 454
BstFNI CGCG 1 cut(s) 193
BstKTI GATC 4 cut(s) 258, 370, 379, 427
BstMAI GTCTC 3 cut(s) 193, 512, 582
BstMBI GATC 4 cut(s) 255, 367, 376, 424
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNSI RCATGY 2 cut(s) 277, 502
BstPAI GACNNNNGTC 1 cut(s) 574
BstSCI CCNGG 2 cut(s) 179, 436
BstSNI TACGTA 1 cut(s) 832
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 888
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
Bsu15I ATCGAT 2 cut(s) 616, 641
BsuRI GGCC 4 cut(s) 169, 309, 324, 488
BsuTUI ATCGAT 2 cut(s) 616, 641
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 3 cut(s) 120, 339, 454
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 223
BtuMI TCGCGA 1 cut(s) 193
BveI ACCTGC 1 cut(s) 106
CaiI CAGNNNCTG 1 cut(s) 365
CciI TCATGA 1 cut(s) 580
Cfr13I GGNCC 2 cut(s) 293, 440
ClaI ATCGAT 2 cut(s) 616, 641
Csp6I GTAC 2 cut(s) 621, 678
CviAII CATG 7 cut(s) 274, 344, 422, 499, 581, 686, 805
CviQI GTAC 2 cut(s) 621, 678
DdeI CTNAG 2 cut(s) 843, 863
DpnI GATC 4 cut(s) 257, 369, 378, 426
DpnII GATC 4 cut(s) 255, 367, 376, 424
DrdI GACNNNNNNGTC 1 cut(s) 725
DseDI GACNNNNNNGTC 1 cut(s) 725
EaeI YGGCCR 4 cut(s) 167, 307, 322, 486
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco105I TACGTA 1 cut(s) 832
Eco130I CCWWGG 2 cut(s) 54, 443
Eco32I GATATC 1 cut(s) 645
Eco47I GGWCC 2 cut(s) 293, 440
Eco57I CTGAAG 1 cut(s) 545
EcoO109I RGGNCCY 1 cut(s) 293
EcoRI GAATTC 2 cut(s) 16, 416
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 2 cut(s) 54, 443
ErhI CCWWGG 2 cut(s) 54, 443
FaeI CATG 7 cut(s) 277, 347, 425, 502, 584, 689, 808
FaqI GGGAC 2 cut(s) 306, 795
FatI CATG 7 cut(s) 273, 343, 421, 498, 580, 685, 804
FblI GTMKAC 2 cut(s) 704, 746
Fnu4HI GCNGC 1 cut(s) 877
FokI GGATG 3 cut(s) 107, 326, 461
Fsp4HI GCNGC 1 cut(s) 877
FspBI CTAG 1 cut(s) 503
GluI GCNGC 1 cut(s) 877
GsuI CTGGAG 1 cut(s) 536
HaeIII GGCC 4 cut(s) 169, 309, 324, 488
HapII CCGG 2 cut(s) 180, 438
Hin1II CATG 7 cut(s) 277, 347, 425, 502, 584, 689, 808
HincII GTYRAC 4 cut(s) 674, 705, 718, 747
HindII GTYRAC 4 cut(s) 674, 705, 718, 747
HinfI GANTC 4 cut(s) 61, 101, 520, 536
HpaII CCGG 2 cut(s) 180, 438
HphI GGTGA 2 cut(s) 25, 597
Hpy166II GTNNAC 6 cut(s) 496, 674, 705, 718, 747, 817
Hpy188I TCNGA 4 cut(s) 260, 367, 525, 844
Hpy188III TCNNGA 4 cut(s) 192, 380, 428, 581
Hpy8I GTNNAC 6 cut(s) 496, 674, 705, 718, 747, 817
HpyAV CCTTC 1 cut(s) 406
HpyCH4III ACNGT 5 cut(s) 38, 218, 287, 671, 744
HpyCH4IV ACGT 3 cut(s) 373, 676, 831
HpyCH4V TGCA 5 cut(s) 115, 137, 347, 636, 876
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 2 cut(s) 843, 863
HpySE526I ACGT 3 cut(s) 373, 676, 831
Hsp92II CATG 7 cut(s) 277, 347, 425, 502, 584, 689, 808
Kzo9I GATC 4 cut(s) 255, 367, 376, 424
Lsp1109I GCAGC 1 cut(s) 888
LweI GCATC 2 cut(s) 371, 423
MaeI CTAG 1 cut(s) 503
MaeII ACGT 3 cut(s) 373, 676, 831
MaeIII GTNAC 3 cut(s) 603, 827, 886
MalI GATC 4 cut(s) 257, 369, 378, 426
MboI GATC 4 cut(s) 255, 367, 376, 424
MboII GAAGA 4 cut(s) 344, 520, 538, 829
MflI RGATCY 1 cut(s) 255
MlsI TGGCCA 2 cut(s) 169, 488
MluCI AATT 3 cut(s) 16, 416, 544
MluNI TGGCCA 2 cut(s) 169, 488
MlyI GAGTC 1 cut(s) 514
MnlI CCTC 6 cut(s) 135, 214, 223, 306, 467, 787
Mox20I TGGCCA 2 cut(s) 169, 488
MroXI GAANNNNTTC 1 cut(s) 416
MscI TGGCCA 2 cut(s) 169, 488
MseI TTAA 2 cut(s) 693, 857
MslI CAYNNNNRTG 1 cut(s) 585
Msp20I TGGCCA 2 cut(s) 169, 488
MspA1I CMGCKG 1 cut(s) 879
MspI CCGG 2 cut(s) 180, 438
MspR9I CCNGG 2 cut(s) 181, 438
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 398
NciI CCSGG 2 cut(s) 181, 438
NdeII GATC 4 cut(s) 255, 367, 376, 424
NlaIII CATG 7 cut(s) 277, 347, 425, 502, 584, 689, 808
NlaIV GGNNCC 2 cut(s) 7, 294
NmeAIII GCCGAG 1 cut(s) 350
NmuCI GTSAC 2 cut(s) 603, 886
NruI TCGCGA 1 cut(s) 193
NspI RCATGY 2 cut(s) 277, 502
NspV TTCGAA 1 cut(s) 156
PagI TCATGA 1 cut(s) 580
PciI ACATGT 1 cut(s) 273
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 3 cut(s) 61, 101, 536
PflFI GACNNNGTC 1 cut(s) 297
PkrI GCNGC 1 cut(s) 878
PleI GAGTC 1 cut(s) 514
PpsI GAGTC 1 cut(s) 514
Ppu21I YACGTR 2 cut(s) 374, 832
PpuMI RGGWCCY 1 cut(s) 293
PscI ACATGT 1 cut(s) 273
PshAI GACNNNNGTC 1 cut(s) 574
Psp5II RGGWCCY 1 cut(s) 293
PspN4I GGNNCC 2 cut(s) 7, 294
PspPI GGNCC 2 cut(s) 293, 440
PspPPI RGGWCCY 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 1 cut(s) 255
PsyI GACNNNGTC 1 cut(s) 297
PvuII CAGCTG 1 cut(s) 879
RruI TCGCGA 1 cut(s) 193
RsaI GTAC 2 cut(s) 622, 679
RsaNI GTAC 2 cut(s) 621, 678
RseI CAYNNNNRTG 1 cut(s) 585
SalI GTCGAC 2 cut(s) 703, 745
SaqAI TTAA 2 cut(s) 693, 857
SatI GCNGC 1 cut(s) 877
Sau3AI GATC 4 cut(s) 255, 367, 376, 424
Sau96I GGNCC 2 cut(s) 293, 440
SchI GAGTC 1 cut(s) 514
ScrFI CCNGG 2 cut(s) 181, 438
SfaNI GCATC 2 cut(s) 371, 423
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 2 cut(s) 293, 440
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 2 cut(s) 128, 428
SmoI CTYRAG 2 cut(s) 128, 428
SnaBI TACGTA 1 cut(s) 832
Sse9I AATT 3 cut(s) 16, 416, 544
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 2 cut(s) 179, 436
StyI CCWWGG 2 cut(s) 54, 443
TaaI ACNGT 5 cut(s) 38, 218, 287, 671, 744
TaiI ACGT 3 cut(s) 376, 679, 834
TaqI TCGA 7 cut(s) 156, 616, 641, 704, 746, 762, 792
TasI AATT 3 cut(s) 16, 416, 544
TatI WGTACW 1 cut(s) 620
TfiI GAWTC 3 cut(s) 61, 101, 536
Tru1I TTAA 2 cut(s) 693, 857
Tru9I TTAA 2 cut(s) 693, 857
TscAI CASTG 2 cut(s) 112, 223
TseFI GTSAC 2 cut(s) 603, 886
TseI GCWGC 1 cut(s) 876
Tsp45I GTSAC 2 cut(s) 603, 886
TspDTI ATGAA 3 cut(s) 328, 425, 603
TspGWI ACGGA 1 cut(s) 364
TspRI CASTG 2 cut(s) 112, 223
Tth111I GACNNNGTC 1 cut(s) 297
VpaK11BI GGWCC 2 cut(s) 293, 440
XapI RAATTY 3 cut(s) 16, 416, 544
XceI RCATGY 2 cut(s) 277, 502
XmiI GTMKAC 2 cut(s) 704, 746
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.