Rh7DG159000

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0002182
Physical Location & Seq
Forward (+)
14025806 .. 14029041
3236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG159000.1

Sequence Viewer

Length: 570 bp
ATGGGTGCCGTGGTGAATTCCATAGATAAAGACGACCGTATTCCAAAAGAAGCCAAGGAGATTCTACAATCATTGGCTACCAAGTGGGAAAATGTAGCTGATTCCACTGCCTTGCAGGTCATCCCTCTCAAGGGTGCAATGACTAACGAGGTGTTCGAAATAAAGTGGCCAACAAGCACCGGGGAAGTCTCGCGAAAGGTTGTGGTTAGGATTTACAGTGAGGGTGTTGAGGTGTTCTTTGACAGGGATAATGAGATCCGAACATTTGAATATATGTCAAAGAACGGTCAGGGACCTCGTCTGCTTGGCCGTTTTCCTAATGGCCGAGTTGAAGAGTTCATCCATGCACAGACACTTTCAGCATCTGATCTACGTGATCCAGATATATCTGCTCTTATAGCTACTAAAATGAAGGAATTCCATGATCTTGAGATGCCCGGTCCAAAGGATGTCGTCCTCTGGGGCAGATTGAGGCTCATGGAGTTTGATATATGTGGCCATCGTAAACATGCTAGAGAAGAAGTTGGAGACTCTGAAGAACAAGGAATCTGGGAATTTTATAGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

189

Amino Acids

21.59

Weight (kDa)

5.24

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_kinase PF01633 41 - 158 6.7e-36 Choline/ethanolamine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 5
AccII CGCG 1 cut(s) 193
AclWI GGATC 2 cut(s) 250, 371
AcoI YGGCCR 4 cut(s) 167, 307, 322, 496
AcsI RAATTY 3 cut(s) 16, 416, 554
AcuI CTGAAG 1 cut(s) 555
AfiI CCNNNNNNNGG 2 cut(s) 130, 131
AgsI TTSAA 2 cut(s) 269, 332
AluBI AGCT 2 cut(s) 98, 401
AluI AGCT 2 cut(s) 98, 401
Alw26I GTCTC 2 cut(s) 193, 522
AlwI GGATC 2 cut(s) 250, 371
AlwNI CAGNNNCTG 1 cut(s) 365
AoxI GGCC 4 cut(s) 167, 307, 322, 496
ApoI RAATTY 3 cut(s) 16, 416, 554
Asp700I GAANNNNTTC 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 293, 440
AsuC2I CCSGG 2 cut(s) 181, 438
AsuHPI GGTGA 1 cut(s) 25
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 2 cut(s) 293, 440
BalI TGGCCA 2 cut(s) 169, 498
BanI GGYRCC 1 cut(s) 5
BccI CCATC 1 cut(s) 507
BceAI ACGGC 1 cut(s) 294
BcnI CCSGG 2 cut(s) 181, 438
BcoDI GTCTC 2 cut(s) 193, 522
BfaI CTAG 1 cut(s) 513
BfuAI ACCTGC 1 cut(s) 106
Bme1390I CCNGG 2 cut(s) 181, 438
Bme18I GGWCC 2 cut(s) 293, 440
BmgT120I GGNCC 2 cut(s) 293, 440
BmiI GGNNCC 2 cut(s) 7, 294
BmrFI CCNGG 2 cut(s) 181, 438
BmsI GCATC 2 cut(s) 371, 423
Bpu14I TTCGAA 1 cut(s) 156
BpuEI CTTGAG 2 cut(s) 113, 449
BpuMI CCSGG 2 cut(s) 181, 438
BsaAI YACGTR 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 9, 54, 180
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 131
Bse3DI GCAATG 1 cut(s) 144
BseDI CCNNGG 3 cut(s) 9, 54, 180
BseGI GGATG 3 cut(s) 120, 339, 454
BseLI CCNNNNNNNGG 2 cut(s) 130, 131
BseMI GCAATG 1 cut(s) 144
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 4 cut(s) 169, 309, 324, 498
BshNI GGYRCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 2 cut(s) 180, 438
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 2 cut(s) 130, 131
BsmAI GTCTC 2 cut(s) 193, 522
BsmFI GGGAC 1 cut(s) 306
BsnI GGCC 4 cut(s) 169, 309, 324, 498
Bsp119I TTCGAA 1 cut(s) 156
Bsp143I GATC 4 cut(s) 255, 367, 376, 424
Bsp68I TCGCGA 1 cut(s) 193
BspANI GGCC 4 cut(s) 169, 309, 324, 498
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 2 cut(s) 7, 294
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 2 cut(s) 250, 371
BspT104I TTCGAA 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 144
BssECI CCNNGG 3 cut(s) 9, 54, 180
BssMI GATC 4 cut(s) 255, 367, 376, 424
BssT1I CCWWGG 1 cut(s) 54
Bst4CI ACNGT 3 cut(s) 38, 218, 287
Bst6I CTCTTC 1 cut(s) 327
BstBAI YACGTR 1 cut(s) 374
BstBI TTCGAA 1 cut(s) 156
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 3 cut(s) 120, 339, 454
BstFNI CGCG 1 cut(s) 193
BstKTI GATC 4 cut(s) 258, 370, 379, 427
BstMAI GTCTC 2 cut(s) 193, 522
BstMBI GATC 4 cut(s) 255, 367, 376, 424
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNSI RCATGY 1 cut(s) 512
BstSCI CCNGG 2 cut(s) 179, 436
BstUI CGCG 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BsuRI GGCC 4 cut(s) 169, 309, 324, 498
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 3 cut(s) 120, 339, 454
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 223
BtuMI TCGCGA 1 cut(s) 193
BveI ACCTGC 1 cut(s) 106
CaiI CAGNNNCTG 1 cut(s) 365
Cfr13I GGNCC 2 cut(s) 293, 440
CviAII CATG 4 cut(s) 344, 422, 478, 509
CviJI RGCY 9 cut(s) 53, 77, 98, 169, 309, 324, 401, 475, 498
CviKI_1 RGCY 9 cut(s) 53, 77, 98, 169, 309, 324, 401, 475, 498
DpnI GATC 4 cut(s) 257, 369, 378, 426
DpnII GATC 4 cut(s) 255, 367, 376, 424
EaeI YGGCCR 4 cut(s) 167, 307, 322, 496
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 54
Eco47I GGWCC 2 cut(s) 293, 440
Eco57I CTGAAG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 293
EcoRI GAATTC 2 cut(s) 16, 416
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 4 cut(s) 347, 425, 481, 512
FaqI GGGAC 1 cut(s) 306
FatI CATG 4 cut(s) 343, 421, 477, 508
FokI GGATG 3 cut(s) 107, 326, 461
FspBI CTAG 1 cut(s) 513
HaeIII GGCC 4 cut(s) 169, 309, 324, 498
HapII CCGG 2 cut(s) 180, 438
Hin1II CATG 4 cut(s) 347, 425, 481, 512
HinfI GANTC 4 cut(s) 61, 101, 530, 546
HpaII CCGG 2 cut(s) 180, 438
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 506
Hpy188I TCNGA 3 cut(s) 260, 367, 535
Hpy188III TCNNGA 3 cut(s) 192, 380, 428
Hpy8I GTNNAC 1 cut(s) 506
HpyAV CCTTC 1 cut(s) 406
HpyCH4III ACNGT 3 cut(s) 38, 218, 287
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 3 cut(s) 115, 137, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 4 cut(s) 347, 425, 481, 512
Kzo9I GATC 4 cut(s) 255, 367, 376, 424
LpnPI CCDG 8 cut(s) 101, 193, 229, 275, 393, 445, 451, 535
LweI GCATC 2 cut(s) 371, 423
MaeI CTAG 1 cut(s) 513
MaeII ACGT 1 cut(s) 373
MalI GATC 4 cut(s) 257, 369, 378, 426
MboI GATC 4 cut(s) 255, 367, 376, 424
MboII GAAGA 3 cut(s) 344, 530, 548
MflI RGATCY 1 cut(s) 255
MlsI TGGCCA 2 cut(s) 169, 498
MluCI AATT 3 cut(s) 16, 416, 554
MluNI TGGCCA 2 cut(s) 169, 498
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 7 cut(s) 135, 142, 214, 223, 306, 465, 467
Mox20I TGGCCA 2 cut(s) 169, 498
MroXI GAANNNNTTC 1 cut(s) 416
MscI TGGCCA 2 cut(s) 169, 498
Msp20I TGGCCA 2 cut(s) 169, 498
MspI CCGG 2 cut(s) 180, 438
MspR9I CCNGG 2 cut(s) 181, 438
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 398
NciI CCSGG 2 cut(s) 181, 438
NdeII GATC 4 cut(s) 255, 367, 376, 424
NlaIII CATG 4 cut(s) 347, 425, 481, 512
NlaIV GGNNCC 2 cut(s) 7, 294
NmeAIII GCCGAG 1 cut(s) 350
NruI TCGCGA 1 cut(s) 193
NspI RCATGY 1 cut(s) 512
NspV TTCGAA 1 cut(s) 156
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 3 cut(s) 61, 101, 546
PflFI GACNNNGTC 1 cut(s) 297
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
Ppu21I YACGTR 1 cut(s) 374
PpuMI RGGWCCY 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 293
PspN4I GGNNCC 2 cut(s) 7, 294
PspPI GGNCC 2 cut(s) 293, 440
PspPPI RGGWCCY 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 1 cut(s) 255
PsyI GACNNNGTC 1 cut(s) 297
RruI TCGCGA 1 cut(s) 193
Sau3AI GATC 4 cut(s) 255, 367, 376, 424
Sau96I GGNCC 2 cut(s) 293, 440
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 2 cut(s) 181, 438
SetI ASST 8 cut(s) 100, 120, 153, 201, 234, 298, 376, 403
SfaNI GCATC 2 cut(s) 371, 423
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 2 cut(s) 293, 440
SmlI CTYRAG 2 cut(s) 128, 428
SmoI CTYRAG 2 cut(s) 128, 428
Sse9I AATT 3 cut(s) 16, 416, 554
SspMI CTAG 1 cut(s) 513
StyD4I CCNGG 2 cut(s) 179, 436
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 3 cut(s) 38, 218, 287
TaiI ACGT 1 cut(s) 376
TaqI TCGA 1 cut(s) 156
TasI AATT 3 cut(s) 16, 416, 554
TfiI GAWTC 3 cut(s) 61, 101, 546
TscAI CASTG 2 cut(s) 112, 223
TspDTI ATGAA 2 cut(s) 328, 425
TspRI CASTG 2 cut(s) 112, 223
Tth111I GACNNNGTC 1 cut(s) 297
VpaK11BI GGWCC 2 cut(s) 293, 440
XapI RAATTY 3 cut(s) 16, 416, 554
XceI RCATGY 1 cut(s) 512
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.