Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002182

Physical Location & Seq

Genomic Coordinates Forward (+)

3732335 .. 3746678

Gene Structure 14344 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1043500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1158 bp

ATGGGCGCCATGGAAAATGCCATAGTCAACAGAGAAGATCGTATTCCAGCAGAAGCTAGGGAGATTCTACAATCATTGGCTTCCCAGTGGGATGACGTGGTGGATTCAAAGGCACTGCAGGTGATCCCTCTCAAGGGTGCAATGACAAATGAGGTTTTTGAAATAAAGTGGCCATCAAGGACAGGGGAACTTTCACATAAAGTTGTGGTTAGGATTTATGGTGAGGGTGTCGAGGTGTTCTTTGACAGGGATAATGAGATCCGAACATTTGAACACATGTCAAAGAATGGTCAGGGACCTCGTTTGCTTGGGCGTTTTCCAAATGGCCGAATTGAAGCGTTCATCCACGCACGGACACTCTCAGCATGTGATCTACGTGATCCAGATACATCTGCTCTTATAGCTTCGAAATTGAAGGAGTTCCATGAACTTGATATGCCTGGTCCAAAGGTTGTCACCCTCTGGGATAGATTGCGAAATTGGCTCAGTACGGCCAAGAGATTGTGTACCCCAGAAGAAGCTAATTCCTTTCAGCTGGATTCTATTGAAAACGAAATCCCGTTACTGGAGAGGGAGCTCTCAGGGATTCATGAACGTATGGGTTTTTGCCACAATGATTTACAGTATGGTAACATAATGCTTGAGGAAAAGAGTAAATCGATAACCATAATTGATTATGAGTATGCAAGTTATAATGCTGTACAATTTGACATAGCAAATCATTTCTGTGAGATGGCAGCTGAATATCACACTGATACACCTCATATTTTGGACTACAGCAAATATCCTGGTTTGGAGGAGCGTCGGCGGTTTGTTTGTTTGTACCTGAGTTCTTCAGGCAATCAACCTACTCACAGCGAAGTGGAGCAGCTGGTTCAAGATGTTGAGAACTATACTCTTGCAAGCCATCTTTTTTGGGGCTTATGGGGAATAATATCGGAACATGTGAATGAAATTGACTTTGACTACATGGAATACGCAAGGCAGAGGTTTCAACAGTACTGGACAAGCAGGCCTAAGCTGTTGGGTTCCGTTGGATCTCCTCCAGATATTGTAACTGATGGCGAGCAAAAGGGAACTGAAAAAACATTAGCTAAGCGGCCAAGTATGTTGAAGAAGCTTAAGAAATATTTCAGCTTCACAAAGACGAAAACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231

Pfam Domains

Protein Families

Choline_kin_N Choline_kinase

Protein Analysis

386

Amino Acids

44.37

Weight (kDa)

5.55

Isoelectric Point (pI)

43.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Choline_kinase	PF01633	42 - 335	1.7e-97	Choline/ethanolamine kinase
APH	PF01636	43 - 242	4.7e-09	Phosphotransferase enzyme family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002182)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74320
fragaria_vesca	FvH4_7g03960 FvH4_7g03960
malus_domestica	MD00G1152700.v1.1 MD07G1043500.v1.1
prunus_persica	Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1 Prupe.2G044500_v2.0.a1
pyrus_communis	pycom02g23640 pycom07g03090
rosa_chinensis	RchiOBHm_Chr1g0327661 RchiOBHm_Chr1g0347241 RchiOBHm_Chr2g0110271 RchiOBHm_Chr4g0418131
rosa_laevigata	RLG00000029977
rosa_multiflora	Rmu_sc0000279.1_g000036 Rmu_sc0000869.1_g000001 Rmu_sc0043133.1_g000001 Rmu_ssc0000042.1_g000005
rosa_roxburghii	Rroxscaffold_4G00322480
rosa_rugosa	Rorug01G0069200 Rorug01G0069300
rosa_samantha	Rh1AG086400 Rh1CG083900 Rh1CG275300 Rh1DG090300 Rh2CG220400 Rh3CG286700 Rh5AG054300 Rh5AG520300 Rh7DG159000 Rh7DG257900
rosa_wichuraiana	Rw1G006740 Rw3G022890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	693
AarI	CACCTGC	1 cut(s)	109
Acc36I	ACCTGC	1 cut(s)	109
AccB1I	GGYRCC	1 cut(s)	5
AciI	CCGC	2 cut(s)	808, 1099
AclWI	GGATC	4 cut(s)	118, 253, 374, 1045
AcoI	YGGCCR	4 cut(s)	170, 325, 492, 1100
AcuI	CTGAAG	1 cut(s)	819
AcyI	GRCGYC	1 cut(s)	6
AfaI	GTAC	5 cut(s)	490, 508, 702, 824, 1001
AfiI	CCNNNNNNNGG	3 cut(s)	133, 134, 565
AflII	CTTAAG	1 cut(s)	1121
AflIII	ACRYGT	2 cut(s)	276, 943
AgsI	TTSAA	9 cut(s)	108, 161, 272, 335, 415, 548, 878, 995, 1114
AjiI	CACGTC	1 cut(s)	97
AjnI	CCWGG	2 cut(s)	439, 787
Alw21I	GWGCWC	1 cut(s)	579
AlwI	GGATC	4 cut(s)	118, 253, 374, 1045
AoxI	GGCC	5 cut(s)	170, 325, 492, 1013, 1100
ApeKI	GCWGC	2 cut(s)	737, 868
Asp700I	GAANNNNTTC	2 cut(s)	419, 1130
AspLEI	GCGC	1 cut(s)	8
AspS9I	GGNCC	2 cut(s)	296, 443
AsuHPI	GGTGA	3 cut(s)	133, 233, 448
AsuII	TTCGAA	1 cut(s)	407
AvaII	GGWCC	2 cut(s)	296, 443
BalI	TGGCCA	1 cut(s)	172
BanI	GGYRCC	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	579
Bbv12I	GWGCWC	1 cut(s)	579
BbvI	GCAGC	2 cut(s)	749, 880
BccI	CCATC	4 cut(s)	181, 727, 915, 1055
BceAI	ACGGC	1 cut(s)	507
BciT130I	CCWGG	2 cut(s)	441, 789
BfaI	CTAG	1 cut(s)	57
BfmI	CTRYAG	2 cut(s)	116, 775
BfoI	RGCGCY	1 cut(s)	9
BfrI	CTTAAG	1 cut(s)	1121
BfuAI	ACCTGC	1 cut(s)	109
BisI	GCNGC	3 cut(s)	738, 869, 1100
BlpI	GCTNAGC	1 cut(s)	1095
BlsI	GCNGC	3 cut(s)	739, 870, 1101
BmcAI	AGTACT	1 cut(s)	1001
Bme1390I	CCNGG	2 cut(s)	441, 789
Bme18I	GGWCC	2 cut(s)	296, 443
BmgBI	CACGTC	1 cut(s)	97
BmgT120I	GGNCC	2 cut(s)	296, 443
BmiI	GGNNCC	3 cut(s)	7, 297, 1030
BmrFI	CCNGG	2 cut(s)	441, 789
BmrI	ACTGGG	1 cut(s)	79
BmuI	ACTGGG	1 cut(s)	79
BpmI	CTGGAG	2 cut(s)	587, 1029
Bpu10I	CCTNAGC	1 cut(s)	1017
Bpu1102I	GCTNAGC	1 cut(s)	1095
Bpu14I	TTCGAA	1 cut(s)	407
BpuEI	CTTGAG	2 cut(s)	116, 662
Bsa29I	ATCGAT	1 cut(s)	659
BsaAI	YACGTR	1 cut(s)	377
BsaHI	GRCGYC	1 cut(s)	6
BsaJI	CCNNGG	1 cut(s)	9
Bsc4I	CCNNNNNNNGG	3 cut(s)	133, 134, 565
Bse1I	ACTGG	3 cut(s)	85, 570, 1007
Bse3DI	GCAATG	1 cut(s)	147
BseBI	CCWGG	2 cut(s)	441, 789
BseCI	ATCGAT	1 cut(s)	659
BseDI	CCNNGG	1 cut(s)	9
BseGI	GGATG	2 cut(s)	97, 342
BseLI	CCNNNNNNNGG	3 cut(s)	133, 134, 565
BseMI	GCAATG	1 cut(s)	147
BseMII	CTCAG	4 cut(s)	375, 499, 594, 818
BseNI	ACTGG	3 cut(s)	85, 570, 1007
BseRI	GAGGAG	2 cut(s)	812, 1032
BseXI	GCAGC	2 cut(s)	749, 880
BshFI	GGCC	5 cut(s)	172, 327, 494, 1015, 1102
BshNI	GGYRCC	1 cut(s)	5
BshVI	ATCGAT	1 cut(s)	659
BsiHKAI	GWGCWC	1 cut(s)	579
BslFI	GGGAC	1 cut(s)	309
BslI	CCNNNNNNNGG	3 cut(s)	133, 134, 565
BsmFI	GGGAC	1 cut(s)	309
BsnI	GGCC	5 cut(s)	172, 327, 494, 1015, 1102
Bsp119I	TTCGAA	1 cut(s)	407
Bsp1286I	GDGCHC	1 cut(s)	579
Bsp1407I	TGTACA	1 cut(s)	700
Bsp143I	GATC	6 cut(s)	37, 123, 258, 370, 379, 1037
Bsp1720I	GCTNAGC	1 cut(s)	1095
Bsp19I	CCATGG	1 cut(s)	9
BspACI	CCGC	2 cut(s)	808, 1099
BspANI	GGCC	5 cut(s)	172, 327, 494, 1015, 1102
BspCNI	CTCAG	4 cut(s)	374, 498, 593, 819
BspDI	ATCGAT	1 cut(s)	659
BspHI	TCATGA	1 cut(s)	589
BspLI	GGNNCC	3 cut(s)	7, 297, 1030
BspMAI	CTGCAG	1 cut(s)	120
BspMI	ACCTGC	1 cut(s)	109
BspPI	GGATC	4 cut(s)	118, 253, 374, 1045
BspT104I	TTCGAA	1 cut(s)	407
BspT107I	GGYRCC	1 cut(s)	5
BspTI	CTTAAG	1 cut(s)	1121
BsrDI	GCAATG	1 cut(s)	147
BsrGI	TGTACA	1 cut(s)	700
BsrI	ACTGG	3 cut(s)	85, 570, 1007
BssECI	CCNNGG	1 cut(s)	9
BssMI	GATC	6 cut(s)	37, 123, 258, 370, 379, 1037
BssNI	GRCGYC	1 cut(s)	6
BssT1I	CCWWGG	1 cut(s)	9
Bst2UI	CCWGG	2 cut(s)	441, 789
Bst4CI	ACNGT	2 cut(s)	624, 999
BstACI	GRCGYC	1 cut(s)	6
BstAFI	CTTAAG	1 cut(s)	1121
BstAUI	TGTACA	1 cut(s)	700
BstBAI	YACGTR	1 cut(s)	377
BstBI	TTCGAA	1 cut(s)	407
BstC8I	GCNNGC	3 cut(s)	904, 1013, 1067
BstDEI	CTNAG	7 cut(s)	361, 485, 580, 827, 1017, 1095, 1155
BstDSI	CCRYGG	1 cut(s)	9
BstF5I	GGATG	2 cut(s)	97, 342
BstH2I	RGCGCY	1 cut(s)	9
BstHHI	GCGC	1 cut(s)	8
BstKTI	GATC	6 cut(s)	40, 126, 261, 373, 382, 1040
BstMBI	GATC	6 cut(s)	37, 123, 258, 370, 379, 1037
BstMWI	GCNNNNNNNGC	2 cut(s)	401, 481
BstNI	CCWGG	2 cut(s)	441, 789
BstNSI	RCATGY	3 cut(s)	280, 369, 947
BstSCI	CCNGG	2 cut(s)	439, 787
BstSFI	CTRYAG	2 cut(s)	116, 775
BstV1I	GCAGC	2 cut(s)	749, 880
BstX2I	RGATCY	2 cut(s)	258, 1037
BstYI	RGATCY	2 cut(s)	258, 1037
Bsu15I	ATCGAT	1 cut(s)	659
BsuRI	GGCC	5 cut(s)	172, 327, 494, 1015, 1102
BsuTUI	ATCGAT	1 cut(s)	659
BtgI	CCRYGG	1 cut(s)	9
BtrI	CACGTC	1 cut(s)	97
BtsCI	GGATG	2 cut(s)	97, 342
BtsI	GCAGTG	1 cut(s)	113
BtsIMutI	CAGTG	3 cut(s)	92, 113, 750
BveI	ACCTGC	1 cut(s)	109
Cac8I	GCNNGC	3 cut(s)	904, 1013, 1067
CciI	TCATGA	1 cut(s)	589
CfoI	GCGC	1 cut(s)	8
Cfr13I	GGNCC	2 cut(s)	296, 443
ClaI	ATCGAT	1 cut(s)	659
CseI	GACGC	1 cut(s)	791
Csp6I	GTAC	5 cut(s)	489, 507, 701, 823, 1000
CviAII	CATG	7 cut(s)	10, 277, 366, 425, 590, 944, 970
CviQI	GTAC	5 cut(s)	489, 507, 701, 823, 1000
DdeI	CTNAG	7 cut(s)	361, 485, 580, 827, 1017, 1095, 1155
DinI	GGCGCC	1 cut(s)	7
DpnI	GATC	6 cut(s)	39, 125, 260, 372, 381, 1039
DpnII	GATC	6 cut(s)	37, 123, 258, 370, 379, 1037
EaeI	YGGCCR	4 cut(s)	170, 325, 492, 1100
Ecl136II	GAGCTC	1 cut(s)	577
Eco130I	CCWWGG	1 cut(s)	9
Eco147I	AGGCCT	1 cut(s)	1015
Eco24I	GRGCYC	1 cut(s)	579
Eco47I	GGWCC	2 cut(s)	296, 443
Eco53kI	GAGCTC	1 cut(s)	577
Eco57I	CTGAAG	1 cut(s)	819
EcoICRI	GAGCTC	1 cut(s)	577
EcoO109I	RGGNCCY	1 cut(s)	296
EcoRII	CCWGG	2 cut(s)	439, 787
EcoT14I	CCWWGG	1 cut(s)	9
EcoT38I	GRGCYC	1 cut(s)	579
EgeI	GGCGCC	1 cut(s)	7
EheI	GGCGCC	1 cut(s)	7
ErhI	CCWWGG	1 cut(s)	9
FaeI	CATG	7 cut(s)	13, 280, 369, 428, 593, 947, 973
FaqI	GGGAC	1 cut(s)	309
FatI	CATG	7 cut(s)	9, 276, 365, 424, 589, 943, 969
Fnu4HI	GCNGC	3 cut(s)	738, 869, 1100
FokI	GGATG	2 cut(s)	104, 329
FriOI	GRGCYC	1 cut(s)	579
Fsp4HI	GCNGC	3 cut(s)	738, 869, 1100
FspBI	CTAG	1 cut(s)	57
GlaI	GCGC	1 cut(s)	7
GluI	GCNGC	3 cut(s)	738, 869, 1100
GsuI	CTGGAG	2 cut(s)	587, 1029
HaeII	RGCGCY	1 cut(s)	9
HaeIII	GGCC	5 cut(s)	172, 327, 494, 1015, 1102
HgaI	GACGC	1 cut(s)	791
HhaI	GCGC	1 cut(s)	8
Hin1I	GRCGYC	1 cut(s)	6
Hin1II	CATG	7 cut(s)	13, 280, 369, 428, 593, 947, 973
Hin6I	GCGC	1 cut(s)	6
HinP1I	GCGC	1 cut(s)	6
HincII	GTYRAC	1 cut(s)	28
HindII	GTYRAC	1 cut(s)	28
HindIII	AAGCTT	1 cut(s)	1118
HinfI	GANTC	4 cut(s)	64, 104, 539, 586
HphI	GGTGA	3 cut(s)	133, 233, 448
Hpy166II	GTNNAC	2 cut(s)	28, 507
Hpy188I	TCNGA	2 cut(s)	263, 940
Hpy188III	TCNNGA	4 cut(s)	383, 590, 878, 1046
Hpy8I	GTNNAC	2 cut(s)	28, 507
Hpy99I	CGWCG	1 cut(s)	807
HpyAV	CCTTC	1 cut(s)	409
HpyCH4III	ACNGT	2 cut(s)	624, 999
HpyCH4IV	ACGT	3 cut(s)	96, 376, 595
HpyCH4V	TGCA	4 cut(s)	118, 140, 686, 902
HpyF10VI	GCNNNNNNNGC	2 cut(s)	401, 481
HpyF3I	CTNAG	7 cut(s)	361, 485, 580, 827, 1017, 1095, 1155
HpySE526I	ACGT	3 cut(s)	96, 376, 595
Hsp92I	GRCGYC	1 cut(s)	6
Hsp92II	CATG	7 cut(s)	13, 280, 369, 428, 593, 947, 973
HspAI	GCGC	1 cut(s)	6
KasI	GGCGCC	1 cut(s)	5
Kzo9I	GATC	6 cut(s)	37, 123, 258, 370, 379, 1037
LmnI	GCTCC	3 cut(s)	574, 799, 865
Lsp1109I	GCAGC	2 cut(s)	749, 880
MaeI	CTAG	1 cut(s)	57
MaeII	ACGT	3 cut(s)	96, 376, 595
MaeIII	GTNAC	4 cut(s)	454, 561, 629, 1054
MalI	GATC	6 cut(s)	39, 125, 260, 372, 381, 1039
MboI	GATC	6 cut(s)	37, 123, 258, 370, 379, 1037
MboII	GAAGA	4 cut(s)	47, 527, 825, 1126
MflI	RGATCY	2 cut(s)	258, 1037
MhlI	GDGCHC	1 cut(s)	579
MlsI	TGGCCA	1 cut(s)	172
MluCI	AATT	7 cut(s)	330, 410, 478, 523, 669, 704, 954
MluNI	TGGCCA	1 cut(s)	172
Mly113I	GGCGCC	1 cut(s)	6
MmeI	TCCRAC	1 cut(s)	1015
Mox20I	TGGCCA	1 cut(s)	172
MroXI	GAANNNNTTC	2 cut(s)	419, 1130
MscI	TGGCCA	1 cut(s)	172
MseI	TTAA	1 cut(s)	1122
MslI	CAYNNNNRTG	2 cut(s)	726, 948
Msp20I	TGGCCA	1 cut(s)	172
MspA1I	CMGCKG	3 cut(s)	535, 740, 871
MspCI	CTTAAG	1 cut(s)	1121
MspR9I	CCNGG	2 cut(s)	441, 789
MvaI	CCWGG	2 cut(s)	441, 789
MwoI	GCNNNNNNNGC	2 cut(s)	401, 481
NarI	GGCGCC	1 cut(s)	6
NcoI	CCATGG	1 cut(s)	9
NdeII	GATC	6 cut(s)	37, 123, 258, 370, 379, 1037
NlaIII	CATG	7 cut(s)	13, 280, 369, 428, 593, 947, 973
NlaIV	GGNNCC	3 cut(s)	7, 297, 1030
NmuCI	GTSAC	1 cut(s)	454
NspI	RCATGY	3 cut(s)	280, 369, 947
NspV	TTCGAA	1 cut(s)	407
PagI	TCATGA	1 cut(s)	589
PaqCI	CACCTGC	1 cut(s)	109
PceI	AGGCCT	1 cut(s)	1015
PciI	ACATGT	2 cut(s)	276, 943
PdmI	GAANNNNTTC	2 cut(s)	419, 1130
PfeI	GAWTC	4 cut(s)	64, 104, 539, 586
PkrI	GCNGC	3 cut(s)	739, 870, 1101
PluTI	GGCGCC	1 cut(s)	9
Ppu21I	YACGTR	1 cut(s)	377
PpuMI	RGGWCCY	1 cut(s)	296
PscI	ACATGT	2 cut(s)	276, 943
PsiI	TTATAA	1 cut(s)	693
Psp124BI	GAGCTC	1 cut(s)	579
Psp5II	RGGWCCY	1 cut(s)	296
Psp6I	CCWGG	2 cut(s)	439, 787
PspGI	CCWGG	2 cut(s)	439, 787
PspN4I	GGNNCC	3 cut(s)	7, 297, 1030
PspPI	GGNCC	2 cut(s)	296, 443
PspPPI	RGGWCCY	1 cut(s)	296
PstI	CTGCAG	1 cut(s)	120
PsuI	RGATCY	2 cut(s)	258, 1037
PvuII	CAGCTG	3 cut(s)	535, 740, 871
RsaI	GTAC	5 cut(s)	490, 508, 702, 824, 1001
RsaNI	GTAC	5 cut(s)	489, 507, 701, 823, 1000
RseI	CAYNNNNRTG	2 cut(s)	726, 948
SacI	GAGCTC	1 cut(s)	579
SaqAI	TTAA	1 cut(s)	1122
SatI	GCNGC	3 cut(s)	738, 869, 1100
Sau3AI	GATC	6 cut(s)	37, 123, 258, 370, 379, 1037
Sau96I	GGNCC	2 cut(s)	296, 443
ScaI	AGTACT	1 cut(s)	1001
ScrFI	CCNGG	2 cut(s)	441, 789
SduI	GDGCHC	1 cut(s)	579
SfcI	CTRYAG	2 cut(s)	116, 775
SfoI	GGCGCC	1 cut(s)	7
SfuI	TTCGAA	1 cut(s)	407
SinI	GGWCC	2 cut(s)	296, 443
SmiMI	CAYNNNNRTG	2 cut(s)	726, 948
SmlI	CTYRAG	3 cut(s)	131, 641, 1121
SmoI	CTYRAG	3 cut(s)	131, 641, 1121
Sse9I	AATT	7 cut(s)	330, 410, 478, 523, 669, 704, 954
SseBI	AGGCCT	1 cut(s)	1015
SsiI	CCGC	2 cut(s)	808, 1099
SspDI	GGCGCC	1 cut(s)	5
SspI	AATATT	1 cut(s)	1130
SspMI	CTAG	1 cut(s)	57
SstI	GAGCTC	1 cut(s)	579
StuI	AGGCCT	1 cut(s)	1015
StyD4I	CCNGG	2 cut(s)	439, 787
StyI	CCWWGG	1 cut(s)	9
TaaI	ACNGT	2 cut(s)	624, 999
TaiI	ACGT	3 cut(s)	99, 379, 598
TaqI	TCGA	3 cut(s)	231, 407, 659
TasI	AATT	7 cut(s)	330, 410, 478, 523, 669, 704, 954
TatI	WGTACW	2 cut(s)	700, 999
TauI	GCSGC	1 cut(s)	1102
TfiI	GAWTC	4 cut(s)	64, 104, 539, 586
Tru1I	TTAA	1 cut(s)	1122
Tru9I	TTAA	1 cut(s)	1122
TscAI	CASTG	3 cut(s)	92, 120, 757
TseFI	GTSAC	1 cut(s)	454
TseI	GCWGC	2 cut(s)	737, 868
Tsp45I	GTSAC	1 cut(s)	454
TspDTI	ATGAA	5 cut(s)	331, 441, 578, 606, 966
TspGWI	ACGGA	2 cut(s)	367, 1021
TspRI	CASTG	3 cut(s)	92, 120, 757
Vha464I	CTTAAG	1 cut(s)	1121
VpaK11BI	GGWCC	2 cut(s)	296, 443
XceI	RCATGY	3 cut(s)	280, 369, 947
XmnI	GAANNNNTTC	2 cut(s)	419, 1130
XspI	CTAG	1 cut(s)	57
ZrmI	AGTACT	1 cut(s)	1001

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.