Rorug01G0069200

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002182
Physical Location & Seq
Forward (+)
11354918 .. 11356048
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0069200.1

Sequence Viewer

Length: 1131 bp
ATGACTTCTGTTGATGCCATGGCCGCTCGGCTGGCTTCTTCTCTAGCCATTGCTGATGGGAGGAAGCAGGTCGACCTGAGACCGACACAGGGGAAGGGTCTGCGGCAGGCAGACGTGTTTTTGGTGGGAAAGCTGCTCACCGCTCGAGAATATAAAACTCGATCTTTTCTGGGGATGTTTCGGAACGCATGGAGGGTACATGGATCTTTGCGTGTGGAGGAAGCGGAGGGGCAGAGGGTTCTCGTCACTTTGTCTGATGCTACAGATCGGGATTGGATCTGGAAGGGAGGTCCATGGGGTTATAATCACGCTCCAATTGCCCTAGCTTACTACGATGGTGTGGCTGACATTGAATCGGTCTCCTTCACCACGTCGCCGTACTGGATCACTTTGCAAGGCATCCCCCCTGCGTTCCGTTCCAAGAGGGTGATGACCATGATCGACTTTACTCTTGGTGATTTTAAGGAAATTGACAAACAGGCCAAGAAGGTTGGAAAGCTTCGAATCAGGGTGGAGATTCCGTTTAACCGGCCACTGTCCTTCCAACGATGGTATTGGGTGGAGGATGAAGTAGAGTTTCTCTGCAAGTTTAAGTTTGACAAACTCTTTGGTCGATGCTCGATTTGTGGTTTGGTTACTCACGTAGGCCTCCCATGCTCGGGGCCTCCTTTGGAGGAGAATGATGAGGCAGAACGCACGGTCCTGGGTGCGTTTCAGGCGAGTAACTCTGCTGCACCGGAGATGCAGCTTGCTGGGCACCTTATCTTGGCCTCAGGCTTGGCTTTGATTAGCACAATGAATGCAGGAGTTCGGGCTAGGGTTTCTTTGGGAGACAAGGACGTTGTTTTCCGTACTGTGCTTCCCAAATCTATGCCGGAGCGACTGCTCCATAATCCGGCATTGCTGATGCACCGACCTCGGCGTCAAGCTCAGATACGAAAACTGCCTGAGATGATGGCTGTACCTGACAGCATCATAGGAAAGAGGAGACTGAGGGAGGATGATACAATCACTATCTCTCCTAAGAAGCTCAAACTGAGCCTTGCTGTTGGAAAAAGAAATTTGAATCTGGAACCGGAACATCTAGGTTGGTACCGGTGCTGCAAACTGAGGAACAGCCCCTCCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

376

Amino Acids

42.37

Weight (kDa)

9.75

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 40 - 109 8.8e-06 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AasI GACNNNNNNGTC 1 cut(s) 921
Acc36I ACCTGC 1 cut(s) 58
Acc65I GGTACC 1 cut(s) 1092
AccB1I GGYRCC 2 cut(s) 756, 1092
AccBSI CCGCTC 2 cut(s) 26, 143
AccI GTMKAC 1 cut(s) 72
AciI CCGC 4 cut(s) 24, 103, 141, 224
AclWI GGATC 3 cut(s) 211, 284, 392
AcoI YGGCCR 2 cut(s) 21, 530
AcsI RAATTY 1 cut(s) 1060
AcyI GRCGYC 1 cut(s) 922
AfaI GTAC 5 cut(s) 198, 380, 853, 963, 1094
AfiI CCNNNNNNNGG 5 cut(s) 89, 658, 659, 766, 895
AflIII ACRYGT 1 cut(s) 114
AgeI ACCGGT 1 cut(s) 1095
AgsI TTSAA 2 cut(s) 353, 1066
AjiI CACGTC 2 cut(s) 115, 372
AjnI CCWGG 2 cut(s) 702, 1124
AloI GAACNNNNNNTCC 3 cut(s) 684, 716, 1106
AluBI AGCT 6 cut(s) 133, 326, 499, 748, 929, 1030
AluI AGCT 6 cut(s) 133, 326, 499, 748, 929, 1030
Alw26I GTCTC 4 cut(s) 73, 364, 825, 982
AlwI GGATC 3 cut(s) 211, 284, 392
Ama87I CYCGRG 2 cut(s) 144, 658
AoxI GGCC 6 cut(s) 21, 480, 530, 646, 662, 768
ApeKI GCWGC 4 cut(s) 133, 731, 745, 1101
ApoI RAATTY 1 cut(s) 1060
ArsI GACNNNNNNTTYG 2 cut(s) 590, 622
AsiGI ACCGGT 1 cut(s) 1095
Asp718I GGTACC 1 cut(s) 1092
AspS9I GGNCC 3 cut(s) 290, 662, 700
AsuHPI GGTGA 4 cut(s) 130, 358, 439, 467
AsuII TTCGAA 1 cut(s) 502
AvaI CYCGRG 2 cut(s) 144, 658
AvaII GGWCC 2 cut(s) 290, 700
AxyI CCTNAGG 1 cut(s) 772
BaeGI GKGCMC 1 cut(s) 759
BanI GGYRCC 2 cut(s) 756, 1092
BbvI GCAGC 4 cut(s) 120, 718, 757, 1088
BccI CCATC 4 cut(s) 50, 329, 543, 949
BceAI ACGGC 1 cut(s) 361
BcgI CGANNNNNNTGC 2 cut(s) 899, 933
BciT130I CCWGG 2 cut(s) 704, 1126
BcoDI GTCTC 4 cut(s) 73, 364, 825, 982
BfaI CTAG 4 cut(s) 44, 323, 816, 1085
BfmI CTRYAG 1 cut(s) 261
BfuAI ACCTGC 1 cut(s) 58
BisI GCNGC 6 cut(s) 24, 104, 134, 732, 746, 1102
BlsI GCNGC 6 cut(s) 25, 105, 135, 733, 747, 1103
Bme1390I CCNGG 2 cut(s) 704, 1126
Bme18I GGWCC 2 cut(s) 290, 700
BmeT110I CYCGRG 2 cut(s) 144, 658
BmgBI CACGTC 2 cut(s) 115, 372
BmgT120I GGNCC 3 cut(s) 290, 662, 700
BmiI GGNNCC 4 cut(s) 663, 758, 1074, 1094
BmrFI CCNGG 2 cut(s) 704, 1126
BmsI GCATC 7 cut(s) 4, 247, 408, 605, 732, 897, 981
BpmI CTGGAG 1 cut(s) 1108
Bpu14I TTCGAA 1 cut(s) 502
BsaAI YACGTR 1 cut(s) 643
BsaHI GRCGYC 1 cut(s) 922
BsaI GGTCTC 2 cut(s) 73, 364
BsaJI CCNNGG 4 cut(s) 18, 293, 703, 917
BsaWI WCCGGW 3 cut(s) 736, 1075, 1095
BsaXI ACNNNNNCTCC 5 cut(s) 506, 536, 1003, 1033, 1106
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 658, 659, 766, 895
Bse118I RCCGGY 2 cut(s) 528, 1095
Bse1I ACTGG 1 cut(s) 386
Bse21I CCTNAGG 1 cut(s) 772
Bse3DI GCAATG 2 cut(s) 48, 899
BseBI CCWGG 2 cut(s) 704, 1126
BseDI CCNNGG 4 cut(s) 18, 293, 703, 917
BseGI GGATG 4 cut(s) 180, 399, 571, 1006
BseLI CCNNNNNNNGG 5 cut(s) 89, 658, 659, 766, 895
BseMI GCAATG 2 cut(s) 48, 899
BseMII CTCAG 7 cut(s) 68, 786, 939, 944, 983, 1028, 1100
BseNI ACTGG 1 cut(s) 386
BseRI GAGGAG 2 cut(s) 689, 1000
BseSI GKGCMC 1 cut(s) 759
BseXI GCAGC 4 cut(s) 120, 718, 757, 1088
BseYI CCCAGC 1 cut(s) 752
BsgI GTGCAG 1 cut(s) 717
BshFI GGCC 6 cut(s) 23, 482, 532, 648, 664, 770
BshNI GGYRCC 2 cut(s) 756, 1092
BshTI ACCGGT 1 cut(s) 1095
BsiHKCI CYCGRG 2 cut(s) 144, 658
BsiSI CCGG 6 cut(s) 529, 737, 875, 896, 1076, 1096
BslI CCNNNNNNNGG 5 cut(s) 89, 658, 659, 766, 895
BsmAI GTCTC 4 cut(s) 73, 364, 825, 982
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 6 cut(s) 23, 482, 532, 648, 664, 770
Bso31I GGTCTC 2 cut(s) 73, 364
BsoBI CYCGRG 2 cut(s) 144, 658
Bsp119I TTCGAA 1 cut(s) 502
Bsp1286I GDGCHC 1 cut(s) 759
Bsp143I GATC 6 cut(s) 161, 203, 265, 276, 384, 438
Bsp19I CCATGG 2 cut(s) 18, 293
BspACI CCGC 4 cut(s) 24, 103, 141, 224
BspANI GGCC 6 cut(s) 23, 482, 532, 648, 664, 770
BspCNI CTCAG 7 cut(s) 69, 785, 940, 943, 984, 1029, 1101
BspLI GGNNCC 4 cut(s) 663, 758, 1074, 1094
BspMI ACCTGC 1 cut(s) 58
BspPI GGATC 3 cut(s) 211, 284, 392
BspT104I TTCGAA 1 cut(s) 502
BspT107I GGYRCC 2 cut(s) 756, 1092
BspTNI GGTCTC 2 cut(s) 73, 364
BsrBI CCGCTC 2 cut(s) 26, 143
BsrDI GCAATG 2 cut(s) 48, 899
BsrFI RCCGGY 2 cut(s) 528, 1095
BsrI ACTGG 1 cut(s) 386
BssAI RCCGGY 2 cut(s) 528, 1095
BssECI CCNNGG 4 cut(s) 18, 293, 703, 917
BssMI GATC 6 cut(s) 161, 203, 265, 276, 384, 438
BssNI GRCGYC 1 cut(s) 922
BssT1I CCWWGG 2 cut(s) 18, 293
Bst2UI CCWGG 2 cut(s) 704, 1126
Bst4CI ACNGT 3 cut(s) 537, 700, 856
BstACI GRCGYC 1 cut(s) 922
BstBAI YACGTR 1 cut(s) 643
BstBI TTCGAA 1 cut(s) 502
BstC8I GCNNGC 3 cut(s) 33, 108, 750
BstDEI CTNAG 8 cut(s) 77, 772, 930, 948, 992, 1023, 1037, 1109
BstDSI CCRYGG 2 cut(s) 18, 293
BstF5I GGATG 4 cut(s) 180, 399, 571, 1006
BstKTI GATC 6 cut(s) 164, 206, 268, 279, 387, 441
BstMAI GTCTC 4 cut(s) 73, 364, 825, 982
BstMBI GATC 6 cut(s) 161, 203, 265, 276, 384, 438
BstMWI GCNNNNNNNGC 6 cut(s) 23, 32, 317, 654, 716, 754
BstNI CCWGG 2 cut(s) 704, 1126
BstSCI CCNGG 2 cut(s) 702, 1124
BstSFI CTRYAG 1 cut(s) 261
BstSLI GKGCMC 1 cut(s) 759
BstV1I GCAGC 4 cut(s) 120, 718, 757, 1088
BstX2I RGATCY 2 cut(s) 203, 276
BstYI RGATCY 2 cut(s) 203, 276
Bsu36I CCTNAGG 1 cut(s) 772
BsuRI GGCC 6 cut(s) 23, 482, 532, 648, 664, 770
BtgI CCRYGG 2 cut(s) 18, 293
BtrI CACGTC 2 cut(s) 115, 372
BtsCI GGATG 4 cut(s) 180, 399, 571, 1006
BtsIMutI CAGTG 1 cut(s) 533
BveI ACCTGC 1 cut(s) 58
Cac8I GCNNGC 3 cut(s) 33, 108, 750
Cfr10I RCCGGY 2 cut(s) 528, 1095
Cfr13I GGNCC 3 cut(s) 290, 662, 700
CseI GACGC 1 cut(s) 911
Csp6I GTAC 5 cut(s) 197, 379, 852, 962, 1093
CspAI ACCGGT 1 cut(s) 1095
CviAII CATG 6 cut(s) 19, 189, 200, 294, 436, 654
CviQI GTAC 5 cut(s) 197, 379, 852, 962, 1093
DdeI CTNAG 8 cut(s) 77, 772, 930, 948, 992, 1023, 1037, 1109
DpnI GATC 6 cut(s) 163, 205, 267, 278, 386, 440
DpnII GATC 6 cut(s) 161, 203, 265, 276, 384, 438
DrdI GACNNNNNNGTC 1 cut(s) 921
DseDI GACNNNNNNGTC 1 cut(s) 921
EaeI YGGCCR 2 cut(s) 21, 530
Eco130I CCWWGG 2 cut(s) 18, 293
Eco147I AGGCCT 1 cut(s) 648
Eco31I GGTCTC 2 cut(s) 73, 364
Eco47I GGWCC 2 cut(s) 290, 700
Eco81I CCTNAGG 1 cut(s) 772
Eco88I CYCGRG 2 cut(s) 144, 658
EcoO109I RGGNCCY 1 cut(s) 662
EcoRII CCWGG 2 cut(s) 702, 1124
EcoT14I CCWWGG 2 cut(s) 18, 293
ErhI CCWWGG 2 cut(s) 18, 293
FaeI CATG 6 cut(s) 22, 192, 203, 297, 439, 657
FatI CATG 6 cut(s) 18, 188, 199, 293, 435, 653
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 6 cut(s) 24, 104, 134, 732, 746, 1102
FokI GGATG 4 cut(s) 187, 386, 578, 1013
Fsp4HI GCNGC 6 cut(s) 24, 104, 134, 732, 746, 1102
FspBI CTAG 4 cut(s) 44, 323, 816, 1085
GluI GCNGC 6 cut(s) 24, 104, 134, 732, 746, 1102
GsaI CCCAGC 1 cut(s) 756
GsuI CTGGAG 1 cut(s) 1108
HaeIII GGCC 6 cut(s) 23, 482, 532, 648, 664, 770
HapII CCGG 6 cut(s) 529, 737, 875, 896, 1076, 1096
HgaI GACGC 1 cut(s) 911
Hin1I GRCGYC 1 cut(s) 922
Hin1II CATG 6 cut(s) 22, 192, 203, 297, 439, 657
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 4 cut(s) 353, 504, 517, 1066
HpaII CCGG 6 cut(s) 529, 737, 875, 896, 1076, 1096
HphI GGTGA 4 cut(s) 130, 358, 439, 467
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 3 cut(s) 183, 256, 933
Hpy188III TCNNGA 4 cut(s) 146, 269, 280, 1070
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 1 cut(s) 376
HpyAV CCTTC 5 cut(s) 88, 277, 373, 481, 550
HpyCH4III ACNGT 3 cut(s) 537, 700, 856
HpyCH4IV ACGT 4 cut(s) 114, 371, 642, 840
HpyCH4V TGCA 7 cut(s) 394, 585, 734, 745, 803, 910, 1104
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 32, 317, 654, 716, 754
HpyF3I CTNAG 8 cut(s) 77, 772, 930, 948, 992, 1023, 1037, 1109
HpySE526I ACGT 4 cut(s) 114, 371, 642, 840
Hsp92I GRCGYC 1 cut(s) 922
Hsp92II CATG 6 cut(s) 22, 192, 203, 297, 439, 657
KpnI GGTACC 1 cut(s) 1096
Kzo9I GATC 6 cut(s) 161, 203, 265, 276, 384, 438
LmnI GCTCC 3 cut(s) 316, 877, 891
Lsp1109I GCAGC 4 cut(s) 120, 718, 757, 1088
LweI GCATC 7 cut(s) 4, 247, 408, 605, 732, 897, 981
MaeI CTAG 4 cut(s) 44, 323, 816, 1085
MaeII ACGT 4 cut(s) 114, 371, 642, 840
MaeIII GTNAC 3 cut(s) 244, 634, 722
MalI GATC 6 cut(s) 163, 205, 267, 278, 386, 440
MbiI CCGCTC 2 cut(s) 26, 143
MboI GATC 6 cut(s) 161, 203, 265, 276, 384, 438
MboII GAAGA 1 cut(s) 30
MfeI CAATTG 1 cut(s) 315
MflI RGATCY 2 cut(s) 203, 276
MhlI GDGCHC 1 cut(s) 759
MluCI AATT 3 cut(s) 315, 468, 1060
MmeI TCCRAC 3 cut(s) 472, 568, 1030
MseI TTAA 3 cut(s) 462, 525, 591
MspI CCGG 6 cut(s) 529, 737, 875, 896, 1076, 1096
MspR9I CCNGG 2 cut(s) 704, 1126
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 2 cut(s) 704, 1126
MwoI GCNNNNNNNGC 6 cut(s) 23, 32, 317, 654, 716, 754
NcoI CCATGG 2 cut(s) 18, 293
NdeII GATC 6 cut(s) 161, 203, 265, 276, 384, 438
NlaIII CATG 6 cut(s) 22, 192, 203, 297, 439, 657
NlaIV GGNNCC 4 cut(s) 663, 758, 1074, 1094
NmeAIII GCCGAG 2 cut(s) 7, 898
NmuCI GTSAC 1 cut(s) 244
NspV TTCGAA 1 cut(s) 502
PaeR7I CTCGAG 1 cut(s) 144
PceI AGGCCT 1 cut(s) 648
PctI GAATGC 1 cut(s) 805
PfeI GAWTC 4 cut(s) 353, 504, 517, 1066
PinAI ACCGGT 1 cut(s) 1095
PkrI GCNGC 6 cut(s) 25, 105, 135, 733, 747, 1103
Ppu21I YACGTR 1 cut(s) 643
PsiI TTATAA 1 cut(s) 303
Psp6I CCWGG 2 cut(s) 702, 1124
PspFI CCCAGC 1 cut(s) 752
PspGI CCWGG 2 cut(s) 702, 1124
PspN4I GGNNCC 4 cut(s) 663, 758, 1074, 1094
PspPI GGNCC 3 cut(s) 290, 662, 700
PsuI RGATCY 2 cut(s) 203, 276
RsaI GTAC 5 cut(s) 198, 380, 853, 963, 1094
RsaNI GTAC 5 cut(s) 197, 379, 852, 962, 1093
SalI GTCGAC 1 cut(s) 71
SaqAI TTAA 3 cut(s) 462, 525, 591
SatI GCNGC 6 cut(s) 24, 104, 134, 732, 746, 1102
Sau3AI GATC 6 cut(s) 161, 203, 265, 276, 384, 438
Sau96I GGNCC 3 cut(s) 290, 662, 700
ScrFI CCNGG 2 cut(s) 704, 1126
SduI GDGCHC 1 cut(s) 759
SfaNI GCATC 7 cut(s) 4, 247, 408, 605, 732, 897, 981
SfcI CTRYAG 1 cut(s) 261
Sfr274I CTCGAG 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 502
SinI GGWCC 2 cut(s) 290, 700
SlaI CTCGAG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
Sse9I AATT 3 cut(s) 315, 468, 1060
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 4 cut(s) 24, 103, 141, 224
SspMI CTAG 4 cut(s) 44, 323, 816, 1085
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 2 cut(s) 702, 1124
StyI CCWWGG 2 cut(s) 18, 293
TaaI ACNGT 3 cut(s) 537, 700, 856
TaiI ACGT 4 cut(s) 117, 374, 645, 843
TaqI TCGA 7 cut(s) 72, 145, 160, 441, 502, 613, 620
TaqII GACCGA 2 cut(s) 97, 346
TasI AATT 3 cut(s) 315, 468, 1060
TauI GCSGC 2 cut(s) 26, 106
TfiI GAWTC 4 cut(s) 353, 504, 517, 1066
Tru1I TTAA 3 cut(s) 462, 525, 591
Tru9I TTAA 3 cut(s) 462, 525, 591
TscAI CASTG 1 cut(s) 540
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 4 cut(s) 133, 731, 745, 1101
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 2 cut(s) 582, 812
TspGWI ACGGA 3 cut(s) 404, 510, 839
TspRI CASTG 1 cut(s) 540
VpaK11BI GGWCC 2 cut(s) 290, 700
XapI RAATTY 1 cut(s) 1060
XhoI CTCGAG 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 4 cut(s) 44, 323, 816, 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.