Prupe.2G044500_v2.0.a1

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0002182
Physical Location & Seq
Reverse (-)
4968977 .. 4976830
7854 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G044500.8

Sequence Viewer

Length: 1158 bp
ATGGGTGCCATGGAAAATGTCATAGAAAACAGAGAAGATCGTTTACCAGCAGAAGCTAGGGAGATTCTTCAATCATTGGCTTCCCAGTGGGAAGATGTGGTTGATTCAAAGGCACTCCAGGTGATCCCTCTCAAGGGTGCAATGACCAATGAGGTTTTCGAAATAAAGTGGCCAGCAAAGACAGGGGAGCTCTCACGCAAAGTTGTGGTTAGGATTTATGGTGAGGGTGTCGATGTGTTCTTTGACAGGGATAATGAGATCCGAACATTTGAGTACATGTCAAAGAATGGTCAGGGACCTCGCTTGCTTGGGCGTTTTCCAAATGGCCGAATTGAAGCGTTCATCCATGCTCGGACACTATCAGCATGTGATCTACGTGATCCAGATATATCTGCTCTTATAGCTGCTAAATTAAAGGAGTTCCATGAACTTAATATGCCTGGCCCAAAGGATGTCACCCTGTGGGATAGATTGCGAAATTGGCTCAGTACGGCTAAGAGATTGTCTACCCCAGAAGAAGCTAATGCCTTTCAGTTAGATTCCATTGAAAAGGAAATCTCTTTATTGGAGAAGGAACTCTCAGGACCTTCTCAAAGTATGGGTTTTTGCCACAATGATTTACAGTACGGTAACATCATGCTCGAGGAAGGGAGTAAATCAATAACCATAATTGACTACGAGTATGCAAGTTATAATGCTGTTGCATTTGACATATCAAATCATTTCTGTGAGATGGCTGCTGACTATCACACCGATACACCTCATATTTTGGACTACAGCAAATATCCTGGTTTGGAGGAGCGCCAGCGGTTTGTTCGTTTGTACCTGAGTTCTTCAGGCAATCACCCTACTGAGAATGAAGTGGAGCAGCTGGTTCAAGATGTTGAGAACTATACTCTTGCAAGCCATCTGTTTTGGGGCTTATGGGGAATAATATCGGAACATGTGAATGATATTGACTTTGACTACATGGAATATGCAAGGCAAAGGTTTCAACAGTACTGGAAAACCAAGCCTAAGCTGTTGGGTTCTGTTGGATCGTTACCAGATGTTGCGACTGATGGCGAACAAAAGGGCACTAAGAAAACATTATCTAAGCGGCCAAGTATGTTCAAGAAGCTTCAGAAATATTTCAGCTTGACAAAGACGAAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

386

Amino Acids

44.16

Weight (kDa)

5.45

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AccB1I GGYRCC 1 cut(s) 5
AccI GTMKAC 1 cut(s) 506
AciI CCGC 2 cut(s) 808, 1099
AclWI GGATC 4 cut(s) 118, 253, 374, 1045
AcoI YGGCCR 3 cut(s) 170, 325, 1100
AcuI CTGAAG 2 cut(s) 819, 1106
AdeI CACNNNGTG 1 cut(s) 462
AfaI GTAC 5 cut(s) 275, 490, 626, 824, 1001
AfiI CCNNNNNNNGG 2 cut(s) 133, 134
AflIII ACRYGT 2 cut(s) 276, 943
AgsI TTSAA 7 cut(s) 71, 108, 335, 548, 878, 995, 1114
AjnI CCWGG 3 cut(s) 117, 439, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AluBI AGCT 8 cut(s) 56, 190, 404, 521, 871, 1021, 1120, 1137
AluI AGCT 8 cut(s) 56, 190, 404, 521, 871, 1021, 1120, 1137
Alw21I GWGCWC 1 cut(s) 192
AlwI GGATC 4 cut(s) 118, 253, 374, 1045
Ama87I CYCGRG 1 cut(s) 641
AoxI GGCC 4 cut(s) 170, 325, 442, 1100
ApeKI GCWGC 3 cut(s) 404, 737, 868
Asp700I GAANNNNTTC 1 cut(s) 1130
AspLEI GCGC 1 cut(s) 804
AspS9I GGNCC 3 cut(s) 296, 443, 584
AsuHPI GGTGA 4 cut(s) 133, 233, 448, 836
AsuII TTCGAA 1 cut(s) 159
AvaI CYCGRG 1 cut(s) 641
AvaII GGWCC 2 cut(s) 296, 584
BaeGI GKGCMC 1 cut(s) 1079
BalI TGGCCA 1 cut(s) 172
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 192
BarI GAAGNNNNNNTAC 2 cut(s) 27, 59
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 391, 724, 880
BccI CCATC 3 cut(s) 727, 915, 1055
BceAI ACGGC 1 cut(s) 507
BciT130I CCWGG 3 cut(s) 119, 441, 789
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 775
BfoI RGCGCY 1 cut(s) 805
BisI GCNGC 4 cut(s) 405, 738, 869, 1100
BlsI GCNGC 4 cut(s) 406, 739, 870, 1101
BmcAI AGTACT 1 cut(s) 1001
Bme1390I CCNGG 3 cut(s) 119, 441, 789
Bme18I GGWCC 2 cut(s) 296, 584
BmeT110I CYCGRG 1 cut(s) 641
BmgT120I GGNCC 3 cut(s) 296, 443, 584
BmiI GGNNCC 2 cut(s) 7, 297
BmrFI CCNGG 3 cut(s) 119, 441, 789
BmrI ACTGGG 1 cut(s) 79
BmuI ACTGGG 1 cut(s) 79
BpmI CTGGAG 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 1017
Bpu14I TTCGAA 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 116
BsaAI YACGTR 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 134
Bse1I ACTGG 2 cut(s) 85, 1007
Bse3DI GCAATG 1 cut(s) 147
BseBI CCWGG 3 cut(s) 119, 441, 789
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 2 cut(s) 342, 457
BseLI CCNNNNNNNGG 2 cut(s) 133, 134
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 4 cut(s) 499, 594, 818, 843
BseNI ACTGG 2 cut(s) 85, 1007
BseRI GAGGAG 1 cut(s) 812
BseSI GKGCMC 1 cut(s) 1079
BseXI GCAGC 3 cut(s) 391, 724, 880
BshFI GGCC 4 cut(s) 172, 327, 444, 1102
BshNI GGYRCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 641
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 2 cut(s) 133, 134
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 4 cut(s) 172, 327, 444, 1102
BsoBI CYCGRG 1 cut(s) 641
Bsp119I TTCGAA 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 192, 1079
Bsp143I GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 2 cut(s) 808, 1099
BspANI GGCC 4 cut(s) 172, 327, 444, 1102
BspCNI CTCAG 4 cut(s) 498, 593, 819, 844
BspLI GGNNCC 2 cut(s) 7, 297
BspPI GGATC 4 cut(s) 118, 253, 374, 1045
BspT104I TTCGAA 1 cut(s) 159
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 147
BsrI ACTGG 2 cut(s) 85, 1007
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
BssT1I CCWWGG 1 cut(s) 9
Bst2UI CCWGG 3 cut(s) 119, 441, 789
Bst4CI ACNGT 3 cut(s) 624, 629, 999
BstBAI YACGTR 1 cut(s) 377
BstBI TTCGAA 1 cut(s) 159
BstC8I GCNNGC 4 cut(s) 174, 305, 806, 904
BstDEI CTNAG 9 cut(s) 485, 495, 580, 827, 852, 1017, 1080, 1095, 1155
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 2 cut(s) 342, 457
BstH2I RGCGCY 1 cut(s) 805
BstHHI GCGC 1 cut(s) 804
BstKTI GATC 6 cut(s) 40, 126, 261, 373, 382, 1040
BstMBI GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
BstMWI GCNNNNNNNGC 2 cut(s) 401, 481
BstNI CCWGG 3 cut(s) 119, 441, 789
BstNSI RCATGY 3 cut(s) 280, 369, 947
BstSCI CCNGG 3 cut(s) 117, 439, 787
BstSFI CTRYAG 1 cut(s) 775
BstSLI GKGCMC 1 cut(s) 1079
BstV1I GCAGC 3 cut(s) 391, 724, 880
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
BsuRI GGCC 4 cut(s) 172, 327, 444, 1102
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 2 cut(s) 342, 457
BtsIMutI CAGTG 1 cut(s) 92
Cac8I GCNNGC 4 cut(s) 174, 305, 806, 904
CfoI GCGC 1 cut(s) 804
Cfr13I GGNCC 3 cut(s) 296, 443, 584
Csp6I GTAC 5 cut(s) 274, 489, 625, 823, 1000
CviAII CATG 8 cut(s) 10, 277, 347, 366, 425, 637, 944, 970
CviQI GTAC 5 cut(s) 274, 489, 625, 823, 1000
DdeI CTNAG 9 cut(s) 485, 495, 580, 827, 852, 1017, 1080, 1095, 1155
DpnI GATC 6 cut(s) 39, 125, 260, 372, 381, 1039
DpnII GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
DraIII CACNNNGTG 1 cut(s) 462
EaeI YGGCCR 3 cut(s) 170, 325, 1100
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 2 cut(s) 296, 584
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 2 cut(s) 819, 1106
Eco88I CYCGRG 1 cut(s) 641
EcoICRI GAGCTC 1 cut(s) 190
EcoO109I RGGNCCY 2 cut(s) 296, 584
EcoRII CCWGG 3 cut(s) 117, 439, 787
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 8 cut(s) 13, 280, 350, 369, 428, 640, 947, 973
FaqI GGGAC 1 cut(s) 309
FatI CATG 8 cut(s) 9, 276, 346, 365, 424, 636, 943, 969
FblI GTMKAC 1 cut(s) 506
Fnu4HI GCNGC 4 cut(s) 405, 738, 869, 1100
FokI GGATG 2 cut(s) 329, 464
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 4 cut(s) 405, 738, 869, 1100
FspBI CTAG 1 cut(s) 57
GlaI GCGC 1 cut(s) 803
GluI GCNGC 4 cut(s) 405, 738, 869, 1100
GsuI CTGGAG 1 cut(s) 101
HaeII RGCGCY 1 cut(s) 805
HaeIII GGCC 4 cut(s) 172, 327, 444, 1102
HhaI GCGC 1 cut(s) 804
Hin1II CATG 8 cut(s) 13, 280, 350, 369, 428, 640, 947, 973
Hin6I GCGC 1 cut(s) 802
HinP1I GCGC 1 cut(s) 802
HindIII AAGCTT 1 cut(s) 1118
HinfI GANTC 3 cut(s) 64, 104, 539
HphI GGTGA 4 cut(s) 133, 233, 448, 836
Hpy166II GTNNAC 2 cut(s) 44, 507
Hpy188I TCNGA 4 cut(s) 263, 354, 940, 1125
Hpy188III TCNNGA 4 cut(s) 383, 582, 878, 1114
Hpy8I GTNNAC 2 cut(s) 44, 507
HpyAV CCTTC 3 cut(s) 565, 597, 641
HpyCH4III ACNGT 3 cut(s) 624, 629, 999
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 5 cut(s) 140, 686, 704, 902, 980
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 481
HpyF3I CTNAG 9 cut(s) 485, 495, 580, 827, 852, 1017, 1080, 1095, 1155
HpySE526I ACGT 1 cut(s) 376
Hsp92II CATG 8 cut(s) 13, 280, 350, 369, 428, 640, 947, 973
HspAI GCGC 1 cut(s) 802
Kzo9I GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
LmnI GCTCC 3 cut(s) 187, 799, 865
Lsp1109I GCAGC 3 cut(s) 391, 724, 880
MaeI CTAG 1 cut(s) 57
MaeII ACGT 1 cut(s) 376
MaeIII GTNAC 3 cut(s) 454, 629, 1041
MalI GATC 6 cut(s) 39, 125, 260, 372, 381, 1039
MboI GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
MboII GAAGA 5 cut(s) 47, 59, 104, 527, 825
MflI RGATCY 1 cut(s) 258
MhlI GDGCHC 2 cut(s) 192, 1079
MlsI TGGCCA 1 cut(s) 172
MluCI AATT 4 cut(s) 330, 410, 478, 669
MluNI TGGCCA 1 cut(s) 172
MmeI TCCRAC 1 cut(s) 1015
MnlI CCTC 7 cut(s) 138, 145, 217, 309, 637, 771, 790
Mox20I TGGCCA 1 cut(s) 172
MroXI GAANNNNTTC 1 cut(s) 1130
MscI TGGCCA 1 cut(s) 172
MseI TTAA 2 cut(s) 413, 432
MslI CAYNNNNRTG 2 cut(s) 726, 948
Msp20I TGGCCA 1 cut(s) 172
MspA1I CMGCKG 2 cut(s) 808, 871
MspR9I CCNGG 3 cut(s) 119, 441, 789
MvaI CCWGG 3 cut(s) 119, 441, 789
MwoI GCNNNNNNNGC 2 cut(s) 401, 481
NcoI CCATGG 1 cut(s) 9
NdeII GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
NlaIII CATG 8 cut(s) 13, 280, 350, 369, 428, 640, 947, 973
NlaIV GGNNCC 2 cut(s) 7, 297
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 3 cut(s) 280, 369, 947
NspV TTCGAA 1 cut(s) 159
PaeR7I CTCGAG 1 cut(s) 641
PciI ACATGT 2 cut(s) 276, 943
PdmI GAANNNNTTC 1 cut(s) 1130
PfeI GAWTC 3 cut(s) 64, 104, 539
PkrI GCNGC 4 cut(s) 406, 739, 870, 1101
Ppu21I YACGTR 1 cut(s) 377
PpuMI RGGWCCY 2 cut(s) 296, 584
PscI ACATGT 2 cut(s) 276, 943
PsiI TTATAA 1 cut(s) 693
Psp124BI GAGCTC 1 cut(s) 192
Psp5II RGGWCCY 2 cut(s) 296, 584
Psp6I CCWGG 3 cut(s) 117, 439, 787
PspGI CCWGG 3 cut(s) 117, 439, 787
PspN4I GGNNCC 2 cut(s) 7, 297
PspPI GGNCC 3 cut(s) 296, 443, 584
PspPPI RGGWCCY 2 cut(s) 296, 584
PspXI VCTCGAGB 1 cut(s) 641
PsuI RGATCY 1 cut(s) 258
PvuII CAGCTG 1 cut(s) 871
RsaI GTAC 5 cut(s) 275, 490, 626, 824, 1001
RsaNI GTAC 5 cut(s) 274, 489, 625, 823, 1000
RseI CAYNNNNRTG 2 cut(s) 726, 948
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 2 cut(s) 413, 432
SatI GCNGC 4 cut(s) 405, 738, 869, 1100
Sau3AI GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
Sau96I GGNCC 3 cut(s) 296, 443, 584
ScaI AGTACT 1 cut(s) 1001
ScrFI CCNGG 3 cut(s) 119, 441, 789
SduI GDGCHC 2 cut(s) 192, 1079
SfcI CTRYAG 1 cut(s) 775
Sfr274I CTCGAG 1 cut(s) 641
SfuI TTCGAA 1 cut(s) 159
SinI GGWCC 2 cut(s) 296, 584
SlaI CTCGAG 1 cut(s) 641
SmiMI CAYNNNNRTG 2 cut(s) 726, 948
SmlI CTYRAG 2 cut(s) 131, 641
SmoI CTYRAG 2 cut(s) 131, 641
Sse9I AATT 4 cut(s) 330, 410, 478, 669
SsiI CCGC 2 cut(s) 808, 1099
SspI AATATT 1 cut(s) 1130
SspMI CTAG 1 cut(s) 57
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 3 cut(s) 117, 439, 787
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 3 cut(s) 624, 629, 999
TaiI ACGT 1 cut(s) 379
TaqI TCGA 3 cut(s) 159, 231, 642
TasI AATT 4 cut(s) 330, 410, 478, 669
TatI WGTACW 2 cut(s) 273, 999
TauI GCSGC 1 cut(s) 1102
TfiI GAWTC 3 cut(s) 64, 104, 539
Tru1I TTAA 2 cut(s) 413, 432
Tru9I TTAA 2 cut(s) 413, 432
TscAI CASTG 1 cut(s) 92
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 3 cut(s) 404, 737, 868
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 3 cut(s) 331, 441, 873
TspRI CASTG 1 cut(s) 92
VpaK11BI GGWCC 2 cut(s) 296, 584
XceI RCATGY 3 cut(s) 280, 369, 947
XhoI CTCGAG 1 cut(s) 641
XmiI GTMKAC 1 cut(s) 506
XmnI GAANNNNTTC 1 cut(s) 1130
XspI CTAG 1 cut(s) 57
ZrmI AGTACT 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.