RchiOBHm_Chr4g0418131

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002182
Physical Location & Seq
Reverse (-)
43600441 .. 43601449
1009 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38814

Sequence Viewer

Length: 468 bp
ATGGGTGCCGTGGTGAATTCCATAGATAAAGACGACCGTATTCCAAAAGAAGCCAAGGAGATTCTACAATCATTGGCTACCAAGTGGGAAAATGTAGCTGATTCCACTGCCTTGCAGGTCATCCCTCTCAAGGGTGCAATGACTAACGAGGTGTTCGAAATAAAGTGGCCAACAAGCACCGTGGAAGTCTCGCGAAAGGTTGTGGTTAGGATTTACGGTGAGGGTGTTGAGGTGTTCTTTGACAGGGATAATGAGATCCGAACATTTGAATACATGTCAAAGAACGGTCAGGGACCTCGTCTGCTTGGCCGTTTTCCTAATGGCCGAGTTGAAGAGTTCATCCATGCACGGACACTTTCAGCATCTGATCTACTTGATCCAGATATATCTGCTCTTATAGCTACTAAAATGAAGGAATTCCATGATCTTGAGATGCCCGGTCCAAAGGATGTCGTCCTCTGGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

155

Amino Acids

17.45

Weight (kDa)

5.13

Isoelectric Point (pI)

30.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_kinase PF01633 41 - 151 1.2e-35 Choline/ethanolamine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 5
AccII CGCG 1 cut(s) 193
AclWI GGATC 2 cut(s) 250, 371
AcoI YGGCCR 3 cut(s) 167, 307, 322
AcsI RAATTY 2 cut(s) 16, 416
AfiI CCNNNNNNNGG 2 cut(s) 130, 131
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 2 cut(s) 269, 332
AluBI AGCT 2 cut(s) 98, 401
AluI AGCT 2 cut(s) 98, 401
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 2 cut(s) 250, 371
AlwNI CAGNNNCTG 1 cut(s) 365
AoxI GGCC 3 cut(s) 167, 307, 322
ApoI RAATTY 2 cut(s) 16, 416
Asp700I GAANNNNTTC 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 293, 440
AsuC2I CCSGG 1 cut(s) 438
AsuHPI GGTGA 2 cut(s) 25, 230
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 2 cut(s) 293, 440
BalI TGGCCA 1 cut(s) 169
BanI GGYRCC 1 cut(s) 5
BceAI ACGGC 1 cut(s) 294
BcnI CCSGG 1 cut(s) 438
BcoDI GTCTC 1 cut(s) 193
BfuAI ACCTGC 1 cut(s) 106
Bme1390I CCNGG 1 cut(s) 438
Bme18I GGWCC 2 cut(s) 293, 440
BmgT120I GGNCC 2 cut(s) 293, 440
BmiI GGNNCC 2 cut(s) 7, 294
BmrFI CCNGG 1 cut(s) 438
BmsI GCATC 2 cut(s) 371, 423
Bpu14I TTCGAA 1 cut(s) 156
BpuEI CTTGAG 2 cut(s) 113, 449
BpuMI CCSGG 1 cut(s) 438
BsaJI CCNNGG 3 cut(s) 9, 54, 180
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 131
Bse3DI GCAATG 1 cut(s) 144
BseDI CCNNGG 3 cut(s) 9, 54, 180
BseGI GGATG 3 cut(s) 120, 339, 454
BseLI CCNNNNNNNGG 2 cut(s) 130, 131
BseMI GCAATG 1 cut(s) 144
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 3 cut(s) 169, 309, 324
BshNI GGYRCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 1 cut(s) 438
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 2 cut(s) 130, 131
BsmAI GTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 306
BsnI GGCC 3 cut(s) 169, 309, 324
Bsp119I TTCGAA 1 cut(s) 156
Bsp143I GATC 4 cut(s) 255, 367, 376, 424
Bsp68I TCGCGA 1 cut(s) 193
BspANI GGCC 3 cut(s) 169, 309, 324
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 2 cut(s) 7, 294
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 2 cut(s) 250, 371
BspT104I TTCGAA 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 144
BssECI CCNNGG 3 cut(s) 9, 54, 180
BssMI GATC 4 cut(s) 255, 367, 376, 424
BssT1I CCWWGG 1 cut(s) 54
Bst4CI ACNGT 4 cut(s) 38, 181, 218, 287
Bst6I CTCTTC 1 cut(s) 327
BstBI TTCGAA 1 cut(s) 156
BstDSI CCRYGG 2 cut(s) 9, 180
BstF5I GGATG 3 cut(s) 120, 339, 454
BstFNI CGCG 1 cut(s) 193
BstKTI GATC 4 cut(s) 258, 370, 379, 427
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 4 cut(s) 255, 367, 376, 424
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 436
BstUI CGCG 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BsuRI GGCC 3 cut(s) 169, 309, 324
BtgI CCRYGG 2 cut(s) 9, 180
BtsCI GGATG 3 cut(s) 120, 339, 454
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 105
BtuMI TCGCGA 1 cut(s) 193
BveI ACCTGC 1 cut(s) 106
CaiI CAGNNNCTG 1 cut(s) 365
Cfr13I GGNCC 2 cut(s) 293, 440
CspCI CAANNNNNGTGG 2 cut(s) 162, 197
CviAII CATG 3 cut(s) 274, 344, 422
CviJI RGCY 8 cut(s) 53, 77, 98, 169, 309, 324, 401, 465
CviKI_1 RGCY 8 cut(s) 53, 77, 98, 169, 309, 324, 401, 465
DpnI GATC 4 cut(s) 257, 369, 378, 426
DpnII GATC 4 cut(s) 255, 367, 376, 424
EaeI YGGCCR 3 cut(s) 167, 307, 322
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 54
Eco47I GGWCC 2 cut(s) 293, 440
EcoO109I RGGNCCY 1 cut(s) 293
EcoRI GAATTC 2 cut(s) 16, 416
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 3 cut(s) 277, 347, 425
FaiI YATR 6 cut(s) 23, 275, 345, 386, 398, 423
FaqI GGGAC 1 cut(s) 306
FatI CATG 3 cut(s) 273, 343, 421
FokI GGATG 3 cut(s) 107, 326, 461
HaeIII GGCC 3 cut(s) 169, 309, 324
HapII CCGG 1 cut(s) 438
Hin1II CATG 3 cut(s) 277, 347, 425
HinfI GANTC 2 cut(s) 61, 101
HpaII CCGG 1 cut(s) 438
HphI GGTGA 2 cut(s) 25, 230
Hpy188I TCNGA 2 cut(s) 260, 367
Hpy188III TCNNGA 3 cut(s) 192, 380, 428
HpyAV CCTTC 1 cut(s) 406
HpyCH4III ACNGT 4 cut(s) 38, 181, 218, 287
HpyCH4V TGCA 3 cut(s) 115, 137, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
Hsp92II CATG 3 cut(s) 277, 347, 425
Kzo9I GATC 4 cut(s) 255, 367, 376, 424
LpnPI CCDG 6 cut(s) 101, 229, 275, 393, 445, 451
LweI GCATC 2 cut(s) 371, 423
MalI GATC 4 cut(s) 257, 369, 378, 426
MboI GATC 4 cut(s) 255, 367, 376, 424
MboII GAAGA 1 cut(s) 344
MflI RGATCY 1 cut(s) 255
MlsI TGGCCA 1 cut(s) 169
MluCI AATT 2 cut(s) 16, 416
MluNI TGGCCA 1 cut(s) 169
MnlI CCTC 6 cut(s) 135, 142, 214, 223, 306, 467
Mox20I TGGCCA 1 cut(s) 169
MroXI GAANNNNTTC 1 cut(s) 416
MscI TGGCCA 1 cut(s) 169
Msp20I TGGCCA 1 cut(s) 169
MspI CCGG 1 cut(s) 438
MspR9I CCNGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 398
NciI CCSGG 1 cut(s) 438
NdeII GATC 4 cut(s) 255, 367, 376, 424
NlaIII CATG 3 cut(s) 277, 347, 425
NlaIV GGNNCC 2 cut(s) 7, 294
NmeAIII GCCGAG 1 cut(s) 350
NruI TCGCGA 1 cut(s) 193
NspI RCATGY 1 cut(s) 277
NspV TTCGAA 1 cut(s) 156
PciI ACATGT 1 cut(s) 273
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 2 cut(s) 61, 101
PflFI GACNNNGTC 1 cut(s) 297
PpuMI RGGWCCY 1 cut(s) 293
PscI ACATGT 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 293
PspN4I GGNNCC 2 cut(s) 7, 294
PspPI GGNCC 2 cut(s) 293, 440
PspPPI RGGWCCY 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 1 cut(s) 255
PsyI GACNNNGTC 1 cut(s) 297
RruI TCGCGA 1 cut(s) 193
Sau3AI GATC 4 cut(s) 255, 367, 376, 424
Sau96I GGNCC 2 cut(s) 293, 440
ScrFI CCNGG 1 cut(s) 438
SetI ASST 7 cut(s) 100, 120, 153, 201, 234, 298, 403
SfaNI GCATC 2 cut(s) 371, 423
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 2 cut(s) 293, 440
SmlI CTYRAG 2 cut(s) 128, 428
SmoI CTYRAG 2 cut(s) 128, 428
Sse9I AATT 2 cut(s) 16, 416
StyD4I CCNGG 1 cut(s) 436
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 4 cut(s) 38, 181, 218, 287
TaqI TCGA 1 cut(s) 156
TasI AATT 2 cut(s) 16, 416
TfiI GAWTC 2 cut(s) 61, 101
TscAI CASTG 1 cut(s) 112
TspDTI ATGAA 2 cut(s) 328, 425
TspGWI ACGGA 1 cut(s) 364
TspRI CASTG 1 cut(s) 112
Tth111I GACNNNGTC 1 cut(s) 297
VpaK11BI GGWCC 2 cut(s) 293, 440
XapI RAATTY 2 cut(s) 16, 416
XceI RCATGY 1 cut(s) 277
XmnI GAANNNNTTC 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.