pycom07g03090

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002182
Physical Location & Seq
Forward (+)
2566744 .. 2572152
5409 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g03090.5

Sequence Viewer

Length: 1104 bp
ATGGGTGCCATGGAAAATGCCTTGGTCAACAGAGAAGATCGTATTCCAGCAGAAGCTAGGGAGATTCTACAATCATTGGCTTCCCAGTGGGATGACGTGGTGGATTCAAAGGCACTGCAGGTGATCCCTCTCAAGGGTGCAATGACAAATGAGGTTTTTGAAATAAAGTGGCCATCAAGGACAGGGGAACTTTCACATAAAGTTGTGGTTAGGATTTATGGTGAGGGTGTCGAGGTGTTCTTTGACAGGGATAATGAGATCCGAACATTTGAACACATGTCAAAGAATGGTCAGGGACCTCGTTTGCTTGGGCGTTTTCCAAATGGCCGAATTGAAGCGTTCATCCACGCACGGACACTCTCAGCATGTGATCTACGTGATCCGGACACATCTGCTCTTATAGCATCGAAATTGAAGGAGTTCCATGAACTTGATATGCCTGGTCCAAAGGTTGTCACCCTCTGGAATAGATTGCGAAATTGGCTCAGTACGGCCAAGAGATTGTGTACCCCAGAAGAAGCTAATTCCTTTCAGCTGGATTCTATTGAAAACGAAATCCCGTTACTGGAGAAGGAGCTCTCAGGGCCTCATCAACGTATGGGTTTTTGCCACAATGATTTACAGTATGGTAACATAATGCTTGAGGAAAAGAGTAAATCGATAACCATAATCGATTATGAGTATGCAAGTTATAATGCTGTACAATTTGACATAGCAAATCATTTCTGTGAGATGGCAGCTGACTATCACACTGATACACCTCATATTTTGGACTACAGCAAATATCCTGGTTTGGAGGAGCGTCAGCGGTTTGTTTGTTTGTACCTGAGTTCTTCAGGCAATCAACCTACTGACAGCGAAGTGGAGCAGCTGGTTCAAGATGTTGAGAACTATACTCTTGCAAGCCATCTCTTTTGGGGCCTATGGGGAATAATATCGGAACATGTGAATGAAATTGACTTTGACTACATGGAATACGCAAGGCAGAGGTTTCATCAGTACTGGATAAGCAGGCCTAAGCTGTTGGGTTCCGTTGGATCTCCTCCAGATATTGTAACTGATGGTAACTGTGCTGCGAGCAAAGGGGAACTGAAAAAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

368

Amino Acids

42.02

Weight (kDa)

5.18

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AarI CACCTGC 1 cut(s) 109
Acc36I ACCTGC 1 cut(s) 109
AccB1I GGYRCC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 1 cut(s) 808
AclWI GGATC 4 cut(s) 118, 253, 374, 1045
AcoI YGGCCR 3 cut(s) 170, 325, 492
AcuI CTGAAG 1 cut(s) 819
AfaI GTAC 5 cut(s) 490, 508, 702, 824, 1001
AfiI CCNNNNNNNGG 3 cut(s) 133, 134, 565
AflIII ACRYGT 2 cut(s) 276, 943
AgsI TTSAA 7 cut(s) 108, 161, 272, 335, 415, 548, 878
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 2 cut(s) 439, 787
AluBI AGCT 7 cut(s) 56, 521, 535, 577, 740, 871, 1021
AluI AGCT 7 cut(s) 56, 521, 535, 577, 740, 871, 1021
Alw21I GWGCWC 1 cut(s) 579
AlwI GGATC 4 cut(s) 118, 253, 374, 1045
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 6 cut(s) 170, 325, 492, 584, 919, 1013
ApeKI GCWGC 3 cut(s) 737, 868, 1073
Asp700I GAANNNNTTC 1 cut(s) 419
AspS9I GGNCC 4 cut(s) 296, 443, 584, 919
AsuHPI GGTGA 3 cut(s) 133, 233, 448
AvaII GGWCC 2 cut(s) 296, 443
BalI TGGCCA 1 cut(s) 172
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 579
Bbv12I GWGCWC 1 cut(s) 579
BbvI GCAGC 3 cut(s) 749, 880, 1060
BccI CCATC 4 cut(s) 181, 727, 915, 1055
BceAI ACGGC 1 cut(s) 507
BciT130I CCWGG 2 cut(s) 441, 789
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 2 cut(s) 116, 775
BfuAI ACCTGC 1 cut(s) 109
BisI GCNGC 3 cut(s) 738, 869, 1074
BlsI GCNGC 3 cut(s) 739, 870, 1075
BmcAI AGTACT 1 cut(s) 1001
Bme1390I CCNGG 2 cut(s) 441, 789
Bme18I GGWCC 2 cut(s) 296, 443
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 4 cut(s) 296, 443, 584, 919
BmiI GGNNCC 4 cut(s) 7, 297, 920, 1030
BmrFI CCNGG 2 cut(s) 441, 789
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 1 cut(s) 413
BmuI ACTGGG 1 cut(s) 79
BpmI CTGGAG 2 cut(s) 587, 1029
Bpu10I CCTNAGC 1 cut(s) 1017
BpuEI CTTGAG 2 cut(s) 116, 662
Bsa29I ATCGAT 2 cut(s) 659, 672
BsaAI YACGTR 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 9, 21
BsaWI WCCGGW 1 cut(s) 382
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 134, 565
Bse1I ACTGG 3 cut(s) 85, 570, 1007
Bse3DI GCAATG 1 cut(s) 147
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 2 cut(s) 441, 789
BseCI ATCGAT 2 cut(s) 659, 672
BseDI CCNNGG 2 cut(s) 9, 21
BseGI GGATG 2 cut(s) 97, 342
BseLI CCNNNNNNNGG 3 cut(s) 133, 134, 565
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 4 cut(s) 375, 499, 594, 818
BseNI ACTGG 3 cut(s) 85, 570, 1007
BseRI GAGGAG 2 cut(s) 812, 1032
BseXI GCAGC 3 cut(s) 749, 880, 1060
BshFI GGCC 6 cut(s) 172, 327, 494, 586, 921, 1015
BshNI GGYRCC 1 cut(s) 5
BshVI ATCGAT 2 cut(s) 659, 672
BsiHKAI GWGCWC 1 cut(s) 579
BsiSI CCGG 1 cut(s) 383
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 3 cut(s) 133, 134, 565
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 6 cut(s) 172, 327, 494, 586, 921, 1015
Bsp1286I GDGCHC 1 cut(s) 579
Bsp13I TCCGGA 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 700
Bsp143I GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 1 cut(s) 808
BspANI GGCC 6 cut(s) 172, 327, 494, 586, 921, 1015
BspCNI CTCAG 4 cut(s) 374, 498, 593, 819
BspDI ATCGAT 2 cut(s) 659, 672
BspEI TCCGGA 1 cut(s) 382
BspLI GGNNCC 4 cut(s) 7, 297, 920, 1030
BspMAI CTGCAG 1 cut(s) 120
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 4 cut(s) 118, 253, 374, 1045
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 147
BsrGI TGTACA 1 cut(s) 700
BsrI ACTGG 3 cut(s) 85, 570, 1007
BssECI CCNNGG 2 cut(s) 9, 21
BssMI GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
BssT1I CCWWGG 2 cut(s) 9, 21
Bst2UI CCWGG 2 cut(s) 441, 789
Bst4CI ACNGT 2 cut(s) 624, 1070
BstAUI TGTACA 1 cut(s) 700
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 904, 1013, 1078
BstDEI CTNAG 5 cut(s) 361, 485, 580, 827, 1017
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 2 cut(s) 97, 342
BstKTI GATC 6 cut(s) 40, 126, 261, 373, 382, 1040
BstMBI GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
BstMWI GCNNNNNNNGC 3 cut(s) 401, 481, 583
BstNI CCWGG 2 cut(s) 441, 789
BstNSI RCATGY 3 cut(s) 280, 369, 947
BstSCI CCNGG 2 cut(s) 439, 787
BstSFI CTRYAG 2 cut(s) 116, 775
BstV1I GCAGC 3 cut(s) 749, 880, 1060
BstX2I RGATCY 2 cut(s) 258, 1037
BstYI RGATCY 2 cut(s) 258, 1037
Bsu15I ATCGAT 2 cut(s) 659, 672
BsuRI GGCC 6 cut(s) 172, 327, 494, 586, 921, 1015
BsuTUI ATCGAT 2 cut(s) 659, 672
BtgI CCRYGG 1 cut(s) 9
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 2 cut(s) 97, 342
BtsI GCAGTG 1 cut(s) 113
BtsIMutI CAGTG 3 cut(s) 92, 113, 750
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 3 cut(s) 904, 1013, 1078
Cfr13I GGNCC 4 cut(s) 296, 443, 584, 919
ClaI ATCGAT 2 cut(s) 659, 672
CseI GACGC 1 cut(s) 791
Csp6I GTAC 5 cut(s) 489, 507, 701, 823, 1000
CviAII CATG 6 cut(s) 10, 277, 366, 425, 944, 970
CviQI GTAC 5 cut(s) 489, 507, 701, 823, 1000
DdeI CTNAG 5 cut(s) 361, 485, 580, 827, 1017
DpnI GATC 6 cut(s) 39, 125, 260, 372, 381, 1039
DpnII GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
EaeI YGGCCR 3 cut(s) 170, 325, 492
Ecl136II GAGCTC 1 cut(s) 577
Eco130I CCWWGG 2 cut(s) 9, 21
Eco147I AGGCCT 1 cut(s) 1015
Eco24I GRGCYC 1 cut(s) 579
Eco47I GGWCC 2 cut(s) 296, 443
Eco53kI GAGCTC 1 cut(s) 577
Eco57I CTGAAG 1 cut(s) 819
EcoICRI GAGCTC 1 cut(s) 577
EcoO109I RGGNCCY 3 cut(s) 296, 584, 919
EcoRII CCWGG 2 cut(s) 439, 787
EcoT14I CCWWGG 2 cut(s) 9, 21
EcoT38I GRGCYC 1 cut(s) 579
ErhI CCWWGG 2 cut(s) 9, 21
FaeI CATG 6 cut(s) 13, 280, 369, 428, 947, 973
FaqI GGGAC 1 cut(s) 309
FatI CATG 6 cut(s) 9, 276, 365, 424, 943, 969
Fnu4HI GCNGC 3 cut(s) 738, 869, 1074
FokI GGATG 2 cut(s) 104, 329
FriOI GRGCYC 1 cut(s) 579
Fsp4HI GCNGC 3 cut(s) 738, 869, 1074
FspBI CTAG 1 cut(s) 57
GluI GCNGC 3 cut(s) 738, 869, 1074
GsuI CTGGAG 2 cut(s) 587, 1029
HaeIII GGCC 6 cut(s) 172, 327, 494, 586, 921, 1015
HapII CCGG 1 cut(s) 383
HgaI GACGC 1 cut(s) 791
Hin1II CATG 6 cut(s) 13, 280, 369, 428, 947, 973
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 3 cut(s) 64, 104, 539
HpaII CCGG 1 cut(s) 383
HphI GGTGA 3 cut(s) 133, 233, 448
Hpy166II GTNNAC 2 cut(s) 28, 507
Hpy188I TCNGA 2 cut(s) 263, 940
Hpy188III TCNNGA 4 cut(s) 383, 463, 878, 1046
Hpy8I GTNNAC 2 cut(s) 28, 507
HpyAV CCTTC 2 cut(s) 409, 565
HpyCH4III ACNGT 2 cut(s) 624, 1070
HpyCH4IV ACGT 3 cut(s) 96, 376, 595
HpyCH4V TGCA 4 cut(s) 118, 140, 686, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 481, 583
HpyF3I CTNAG 5 cut(s) 361, 485, 580, 827, 1017
HpySE526I ACGT 3 cut(s) 96, 376, 595
Hsp92II CATG 6 cut(s) 13, 280, 369, 428, 947, 973
Kpn2I TCCGGA 1 cut(s) 382
Kzo9I GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
LmnI GCTCC 3 cut(s) 574, 799, 865
Lsp1109I GCAGC 3 cut(s) 749, 880, 1060
LweI GCATC 1 cut(s) 413
MaeI CTAG 1 cut(s) 57
MaeII ACGT 3 cut(s) 96, 376, 595
MaeIII GTNAC 5 cut(s) 454, 561, 629, 1054, 1064
MalI GATC 6 cut(s) 39, 125, 260, 372, 381, 1039
MboI GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
MboII GAAGA 3 cut(s) 47, 527, 825
MflI RGATCY 2 cut(s) 258, 1037
MhlI GDGCHC 1 cut(s) 579
MlsI TGGCCA 1 cut(s) 172
MluCI AATT 6 cut(s) 330, 410, 478, 523, 704, 954
MluNI TGGCCA 1 cut(s) 172
MmeI TCCRAC 1 cut(s) 1015
Mox20I TGGCCA 1 cut(s) 172
MroI TCCGGA 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 419
MscI TGGCCA 1 cut(s) 172
MslI CAYNNNNRTG 2 cut(s) 726, 948
Msp20I TGGCCA 1 cut(s) 172
MspA1I CMGCKG 4 cut(s) 535, 740, 808, 871
MspI CCGG 1 cut(s) 383
MspR9I CCNGG 2 cut(s) 441, 789
MvaI CCWGG 2 cut(s) 441, 789
MwoI GCNNNNNNNGC 3 cut(s) 401, 481, 583
NcoI CCATGG 1 cut(s) 9
NdeII GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
NlaIII CATG 6 cut(s) 13, 280, 369, 428, 947, 973
NlaIV GGNNCC 4 cut(s) 7, 297, 920, 1030
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 3 cut(s) 280, 369, 947
PaqCI CACCTGC 1 cut(s) 109
PceI AGGCCT 1 cut(s) 1015
PciI ACATGT 2 cut(s) 276, 943
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 3 cut(s) 64, 104, 539
PkrI GCNGC 3 cut(s) 739, 870, 1075
Ppu21I YACGTR 1 cut(s) 377
PpuMI RGGWCCY 1 cut(s) 296
PscI ACATGT 2 cut(s) 276, 943
PsiI TTATAA 1 cut(s) 693
Psp124BI GAGCTC 1 cut(s) 579
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 439, 787
PspGI CCWGG 2 cut(s) 439, 787
PspN4I GGNNCC 4 cut(s) 7, 297, 920, 1030
PspPI GGNCC 4 cut(s) 296, 443, 584, 919
PspPPI RGGWCCY 1 cut(s) 296
PstI CTGCAG 1 cut(s) 120
PsuI RGATCY 2 cut(s) 258, 1037
PvuII CAGCTG 3 cut(s) 535, 740, 871
RsaI GTAC 5 cut(s) 490, 508, 702, 824, 1001
RsaNI GTAC 5 cut(s) 489, 507, 701, 823, 1000
RseI CAYNNNNRTG 2 cut(s) 726, 948
SacI GAGCTC 1 cut(s) 579
SatI GCNGC 3 cut(s) 738, 869, 1074
Sau3AI GATC 6 cut(s) 37, 123, 258, 370, 379, 1037
Sau96I GGNCC 4 cut(s) 296, 443, 584, 919
ScaI AGTACT 1 cut(s) 1001
ScrFI CCNGG 2 cut(s) 441, 789
SduI GDGCHC 1 cut(s) 579
SfaNI GCATC 1 cut(s) 413
SfcI CTRYAG 2 cut(s) 116, 775
SinI GGWCC 2 cut(s) 296, 443
SmiMI CAYNNNNRTG 2 cut(s) 726, 948
SmlI CTYRAG 2 cut(s) 131, 641
SmoI CTYRAG 2 cut(s) 131, 641
Sse9I AATT 6 cut(s) 330, 410, 478, 523, 704, 954
SseBI AGGCCT 1 cut(s) 1015
SsiI CCGC 1 cut(s) 808
SspMI CTAG 1 cut(s) 57
SstI GAGCTC 1 cut(s) 579
StuI AGGCCT 1 cut(s) 1015
StyD4I CCNGG 2 cut(s) 439, 787
StyI CCWWGG 2 cut(s) 9, 21
TaaI ACNGT 2 cut(s) 624, 1070
TaiI ACGT 3 cut(s) 99, 379, 598
TaqI TCGA 4 cut(s) 231, 407, 659, 672
TasI AATT 6 cut(s) 330, 410, 478, 523, 704, 954
TatI WGTACW 2 cut(s) 700, 999
TfiI GAWTC 3 cut(s) 64, 104, 539
TscAI CASTG 3 cut(s) 92, 120, 757
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 3 cut(s) 737, 868, 1073
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 4 cut(s) 331, 441, 966, 983
TspGWI ACGGA 2 cut(s) 367, 1021
TspRI CASTG 3 cut(s) 92, 120, 757
VpaK11BI GGWCC 2 cut(s) 296, 443
XceI RCATGY 3 cut(s) 280, 369, 947
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 1 cut(s) 57
ZrmI AGTACT 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.