AT1G74690

IQ-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005039
Physical Location & Seq
Reverse (-)
28061159 .. 28064771
3613 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74690.1

Sequence Viewer

Length: 1764 bp
ATGGGGAAGTCTACAAAATGGTTGAAGAATGTATTGCTTGGGAAAAAAACTTCTAAATCTAGTGGTTCCAAAGACAAAGAGAGAGTTGTGAGTGGAAAAGAGGTACTGGTTACTTCAAAGGTGGAAGAATCAGATGTGGTATCAGATCTTCCATCTTTTGAAGTTGCAGAGACCAATACCGTTGATAGAAGTGGTGGTATGTTAGAGACTCAAAATGTAGGACCTGAAGAGATCTCAGATGATGAAATTGAGCTTCCTGAAGGAAAATCAACAGATTCTCAGAATGTTGCACCTGTTCAAGATCATTCATTATCTGATGCAGAGAGAATCCAACGAGAGATTGCAGCAACGTCTGTGCAGGCTGCGTTTAGAGGTTACTTGGCTCGGCGTGCTTTTTGGGCACTCAAAGGCATAATAAGGCTACAAGCGCTTATCCGTGGTCACCTTGTTAGGAGACAAGCTGTTGCAACTCTTTTCTCTGTCATGGGAATTGTCAGGTTGCAAGCATTTGCTCGAGGAAGAGAGATCAGGAAGTCCGACATTGGAGTTCAAGTTTATAGGAAATGCAGATTGCAGCTACTTCAGGGGAACAAGCTTGCAAATCCTACAGATGCTTACCTTGGAATCAAGAAACTAACAGCCAATGCTTTTGCTCAGAAGCTTCTCGCTTCATCACCAAAAGTGCTACCTGTACACGCATATGATACATCCAATCCAAACTCAAACTTGATCTGGTTAGAGAACTGGTCAGCCTCATGCTTCTGGAAACCAGTTCCTCAACCGAAGAAAACGATAAGCAGAAAACCTCAGAACAGGCTTTTAGTCGAGGCTGAGTCTGCTAAACCCAAGAAGAGTGTCCGTAAAGTTCCTGCTTCAAACTTTGAAAGTTCCTCAGTACAGACATCTTTTGAGTTTGAGAAACCCAAACGCAGTTTCCGGAAAGTTTCAAGCCAGTCGATAGAGCCACCCGCTGTCGAAGATCCTCAGATTGAACTTGAAAAGGTTAAACGTAGCTTGAGGAAGGTACATAATCCTGTAGTTGAGAGCTCTATCCAACCTCAACGATCCCCTCGTAAGGAAGTCGAGAAGCCAAAGCTTGGCGTTGAGAAGACAAGGGAATCATCATATCCGCTGGTTCATGAAACTGCAGAAGAACCTGTGAATGTTTGTGATGAAAAGAAAAAGCAAGAAATTTCCGAACAGCCTGAGGAGGAGGTACATGCTCTTGAGATGGAAGTACATACTCCTGGACCATTAGAAACTAATGAAGCACTTGATTCCTCACTGGTCAACCAAATTGACAGCAATGAAAAAGCCATGGTTGAGGAAAAACCCTCAATGGAAAAGGATACCAAAGAAGAAAAGACTCCCAAACCAAACAATAAAGAGAATTCCGCTGGGAAGGAGAACCAGAAATCCAGGAAAAAGGGTTCAGCTACTAGCAAGACTGAGCGTGAAGAGAGTAATGGGCATCACGAGACTAGCCCATCAATCCCAAGCTATATGCAAGCAACTAAATCGGCGAAAGCAAAGCTAAGGCTTCAAGGCTCACCAAAGTCTGCGGAGCAAGATGGAACTGAGAAAGCCACTGTTCCCAGACGTCACTCTCTACCATCTCCAGGTAATGGTAGGATCACTTCTCACTCTCCAAGAACAACGAGACTCGCAAACTCAGGTGACAAAACCGGGAATAAGAAGGAGAAACCTCTTCTGTCGTCCCGAGAAGGAAACGCCAAGACAACTCCAGCAGAGCGGAAGAGGTGA

Protein Analysis

587

Amino Acids

65.2

Weight (kDa)

9.56

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 470 - 562 1.9e-09 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1603
AccB7I CCANNNNNTGG 2 cut(s) 1097, 1625
AccBSI CCGCTC 1 cut(s) 1753
AccI GTMKAC 1 cut(s) 11
AccIII TCCGGA 1 cut(s) 936
AciI CCGC 5 cut(s) 969, 1130, 1395, 1562, 1753
AclWI GGATC 3 cut(s) 974, 1059, 1640
AcsI RAATTY 2 cut(s) 1191, 1390
AcuI CTGAAG 3 cut(s) 246, 279, 566
AcyI GRCGYC 1 cut(s) 1600
AfaI GTAC 6 cut(s) 105, 693, 897, 1026, 1218, 1239
AfeI AGCGCT 1 cut(s) 429
AfiI CCNNNNNNNGG 4 cut(s) 1075, 1097, 1619, 1625
AjnI CCWGG 3 cut(s) 1246, 1418, 1618
AjuI GAANNNNNNNTTGG 2 cut(s) 917, 949
AloI GAACNNNNNNTCC 4 cut(s) 1400, 1414, 1432, 1446
Alw21I GWGCWC 1 cut(s) 1049
Alw26I GTCTC 5 cut(s) 164, 200, 448, 1472, 1654
AlwI GGATC 3 cut(s) 974, 1059, 1640
Ama87I CYCGRG 2 cut(s) 513, 1719
Aor13HI TCCGGA 1 cut(s) 936
Aor51HI AGCGCT 1 cut(s) 429
ApeKI GCWGC 3 cut(s) 344, 362, 574
ApoI RAATTY 2 cut(s) 1191, 1390
AspLEI GCGC 1 cut(s) 430
AspS9I GGNCC 2 cut(s) 221, 1250
AsuC2I CCSGG 1 cut(s) 1687
AsuHPI GGTGA 4 cut(s) 434, 666, 1542, 1688
AvaI CYCGRG 2 cut(s) 513, 1719
AvaII GGWCC 2 cut(s) 221, 1250
AxyI CCTNAGG 1 cut(s) 1206
BaeGI GKGCMC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 1049
BarI GAAGNNNNNNTAC 4 cut(s) 132, 164, 1621, 1653
BauI CACGAG 1 cut(s) 1475
BbsI GAAGAC 1 cut(s) 1115
Bbv12I GWGCWC 1 cut(s) 1049
BbvI GCAGC 3 cut(s) 349, 356, 586
BccI CCATC 5 cut(s) 160, 1225, 1495, 1565, 1621
BcgI CGANNNNNNTGC 2 cut(s) 1500, 1534
BciT130I CCWGG 3 cut(s) 1248, 1420, 1620
BciVI GTATCC 1 cut(s) 1342
BcnI CCSGG 1 cut(s) 1687
BcoDI GTCTC 5 cut(s) 164, 200, 448, 1472, 1654
BfaI CTAG 3 cut(s) 60, 1440, 1482
BfmI CTRYAG 3 cut(s) 606, 1035, 1146
BfoI RGCGCY 1 cut(s) 431
BfuI GTATCC 1 cut(s) 1342
BglII AGATCT 2 cut(s) 145, 231
BisI GCNGC 3 cut(s) 345, 363, 575
BlsI GCNGC 3 cut(s) 346, 364, 576
Bme1390I CCNGG 4 cut(s) 1248, 1420, 1620, 1687
Bme18I GGWCC 2 cut(s) 221, 1250
BmeT110I CYCGRG 2 cut(s) 513, 1719
BmgT120I GGNCC 2 cut(s) 221, 1250
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 4 cut(s) 1248, 1420, 1620, 1687
BmsI GCATC 3 cut(s) 307, 601, 1480
BpiI GAAGAC 1 cut(s) 1115
BpmI CTGGAG 2 cut(s) 1602, 1728
Bpu10I CCTNAGC 1 cut(s) 1535
BpuEI CTTGAG 2 cut(s) 1036, 1247
BpuMI CCSGG 1 cut(s) 1687
BsaHI GRCGYC 1 cut(s) 1600
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 3 cut(s) 436, 619, 1317
BsaWI WCCGGW 1 cut(s) 936
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 4 cut(s) 1075, 1097, 1619, 1625
Bse1I ACTGG 5 cut(s) 111, 749, 770, 952, 1290
Bse21I CCTNAGG 1 cut(s) 1206
Bse3DI GCAATG 1 cut(s) 1312
BseAI TCCGGA 1 cut(s) 936
BseBI CCWGG 3 cut(s) 1248, 1420, 1620
BseDI CCNNGG 3 cut(s) 436, 619, 1317
BseGI GGATG 1 cut(s) 707
BseLI CCNNNNNNNGG 4 cut(s) 1075, 1097, 1619, 1625
BseMI GCAATG 1 cut(s) 1312
BseNI ACTGG 5 cut(s) 111, 749, 770, 952, 1290
BseRI GAGGAG 2 cut(s) 1223, 1226
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 3 cut(s) 349, 356, 586
BseYI CCCAGC 1 cut(s) 1397
BsgI GTGCAG 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 1049
BsiHKCI CYCGRG 2 cut(s) 513, 1719
BsiSI CCGG 2 cut(s) 937, 1686
BslFI GGGAC 1 cut(s) 1702
BslI CCNNNNNNNGG 4 cut(s) 1075, 1097, 1619, 1625
BsmAI GTCTC 5 cut(s) 164, 200, 448, 1472, 1654
BsmFI GGGAC 1 cut(s) 1702
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 2 cut(s) 513, 1719
Bsp1286I GDGCHC 2 cut(s) 403, 1049
Bsp13I TCCGGA 1 cut(s) 936
Bsp1407I TGTACA 1 cut(s) 691
Bsp143I GATC 8 cut(s) 145, 231, 301, 525, 729, 979, 1064, 1632
Bsp19I CCATGG 1 cut(s) 1317
BspACI CCGC 5 cut(s) 969, 1130, 1395, 1562, 1753
BspEI TCCGGA 1 cut(s) 936
BspHI TCATGA 1 cut(s) 1138
BspLI GGNNCC 1 cut(s) 67
BspMAI CTGCAG 1 cut(s) 1150
BspPI GGATC 3 cut(s) 974, 1059, 1640
BspTNI GGTCTC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 1753
BsrDI GCAATG 1 cut(s) 1312
BsrGI TGTACA 1 cut(s) 691
BsrI ACTGG 5 cut(s) 111, 749, 770, 952, 1290
BssECI CCNNGG 3 cut(s) 436, 619, 1317
BssMI GATC 8 cut(s) 145, 231, 301, 525, 729, 979, 1064, 1632
BssNI GRCGYC 1 cut(s) 1600
BssSI CACGAG 1 cut(s) 1475
BssT1I CCWWGG 2 cut(s) 619, 1317
Bst2BI CACGAG 1 cut(s) 1475
Bst2UI CCWGG 3 cut(s) 1248, 1420, 1620
Bst4CI ACNGT 2 cut(s) 181, 1591
Bst6I CTCTTC 6 cut(s) 222, 514, 845, 1452, 1713, 1751
BstACI GRCGYC 1 cut(s) 1600
BstAUI TGTACA 1 cut(s) 691
BstC8I GCNNGC 5 cut(s) 360, 390, 504, 597, 1509
BstDSI CCRYGG 2 cut(s) 436, 1317
BstEII GGTNACC 1 cut(s) 440
BstF5I GGATG 1 cut(s) 707
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 8 cut(s) 148, 234, 304, 528, 732, 982, 1067, 1635
BstMAI GTCTC 5 cut(s) 164, 200, 448, 1472, 1654
BstMBI GATC 8 cut(s) 145, 231, 301, 525, 729, 979, 1064, 1632
BstMWI GCNNNNNNNGC 4 cut(s) 389, 398, 427, 836
BstNI CCWGG 3 cut(s) 1248, 1420, 1620
BstNSI RCATGY 1 cut(s) 1223
BstPI GGTNACC 1 cut(s) 440
BstSCI CCNGG 4 cut(s) 1246, 1418, 1618, 1685
BstSFI CTRYAG 3 cut(s) 606, 1035, 1146
BstSLI GKGCMC 1 cut(s) 403
BstV1I GCAGC 3 cut(s) 349, 356, 586
BstV2I GAAGAC 1 cut(s) 1115
BstX2I RGATCY 3 cut(s) 145, 231, 979
BstYI RGATCY 3 cut(s) 145, 231, 979
Bsu36I CCTNAGG 1 cut(s) 1206
BsuI GTATCC 1 cut(s) 1342
BtgI CCRYGG 2 cut(s) 436, 1317
BtsCI GGATG 1 cut(s) 707
BtsIMutI CAGTG 2 cut(s) 1283, 1587
Cac8I GCNNGC 5 cut(s) 360, 390, 504, 597, 1509
CciI TCATGA 1 cut(s) 1138
CfoI GCGC 1 cut(s) 430
Cfr13I GGNCC 2 cut(s) 221, 1250
Csp6I GTAC 6 cut(s) 104, 692, 896, 1025, 1217, 1238
CviAII CATG 5 cut(s) 484, 756, 1139, 1220, 1318
CviQI GTAC 6 cut(s) 104, 692, 896, 1025, 1217, 1238
DpnI GATC 8 cut(s) 147, 233, 303, 527, 731, 981, 1066, 1634
DpnII GATC 8 cut(s) 145, 231, 301, 525, 729, 979, 1064, 1632
Eam1104I CTCTTC 6 cut(s) 222, 514, 845, 1452, 1713, 1751
EarI CTCTTC 6 cut(s) 222, 514, 845, 1452, 1713, 1751
Ecl136II GAGCTC 1 cut(s) 1047
Eco130I CCWWGG 2 cut(s) 619, 1317
Eco24I GRGCYC 1 cut(s) 1049
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 2 cut(s) 221, 1250
Eco47III AGCGCT 1 cut(s) 429
Eco53kI GAGCTC 1 cut(s) 1047
Eco57I CTGAAG 3 cut(s) 246, 279, 566
Eco81I CCTNAGG 1 cut(s) 1206
Eco88I CYCGRG 2 cut(s) 513, 1719
Eco91I GGTNACC 1 cut(s) 440
EcoICRI GAGCTC 1 cut(s) 1047
EcoO109I RGGNCCY 1 cut(s) 221
EcoO65I GGTNACC 1 cut(s) 440
EcoRI GAATTC 1 cut(s) 1390
EcoRII CCWGG 3 cut(s) 1246, 1418, 1618
EcoT14I CCWWGG 2 cut(s) 619, 1317
EcoT38I GRGCYC 1 cut(s) 1049
ErhI CCWWGG 2 cut(s) 619, 1317
FaeI CATG 5 cut(s) 487, 759, 1142, 1223, 1321
FaqI GGGAC 1 cut(s) 1702
FatI CATG 5 cut(s) 483, 755, 1138, 1219, 1317
FauI CCCGC 1 cut(s) 976
FauNDI CATATG 1 cut(s) 700
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 3 cut(s) 345, 363, 575
FokI GGATG 1 cut(s) 694
FriOI GRGCYC 1 cut(s) 1049
Fsp4HI GCNGC 3 cut(s) 345, 363, 575
FspBI CTAG 3 cut(s) 60, 1440, 1482
GlaI GCGC 1 cut(s) 429
GluI GCNGC 3 cut(s) 345, 363, 575
GsaI CCCAGC 1 cut(s) 1401
GsuI CTGGAG 2 cut(s) 1602, 1728
HaeII RGCGCY 1 cut(s) 431
HapII CCGG 2 cut(s) 937, 1686
HhaI GCGC 1 cut(s) 430
Hin1I GRCGYC 1 cut(s) 1600
Hin1II CATG 5 cut(s) 487, 759, 1142, 1223, 1321
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HincII GTYRAC 1 cut(s) 1291
HindII GTYRAC 1 cut(s) 1291
HindIII AAGCTT 3 cut(s) 593, 659, 1094
HpaII CCGG 2 cut(s) 937, 1686
HphI GGTGA 4 cut(s) 434, 666, 1542, 1688
Hpy166II GTNNAC 3 cut(s) 12, 694, 1291
Hpy8I GTNNAC 3 cut(s) 12, 694, 1291
HpyAV CCTTC 5 cut(s) 254, 1015, 1396, 1690, 1718
HpyCH4III ACNGT 2 cut(s) 181, 1591
HpyCH4IV ACGT 3 cut(s) 350, 1009, 1600
HpyF10VI GCNNNNNNNGC 4 cut(s) 389, 398, 427, 836
HpySE526I ACGT 3 cut(s) 350, 1009, 1600
Hsp92I GRCGYC 1 cut(s) 1600
Hsp92II CATG 5 cut(s) 487, 759, 1142, 1223, 1321
HspAI GCGC 1 cut(s) 428
Kpn2I TCCGGA 1 cut(s) 936
Kzo9I GATC 8 cut(s) 145, 231, 301, 525, 729, 979, 1064, 1632
LmnI GCTCC 1 cut(s) 1564
Lsp1109I GCAGC 3 cut(s) 349, 356, 586
LweI GCATC 3 cut(s) 307, 601, 1480
MaeI CTAG 3 cut(s) 60, 1440, 1482
MaeII ACGT 3 cut(s) 350, 1009, 1600
MaeIII GTNAC 5 cut(s) 109, 374, 440, 1601, 1676
MalI GATC 8 cut(s) 147, 233, 303, 527, 731, 981, 1066, 1634
MbiI CCGCTC 1 cut(s) 1753
MboI GATC 8 cut(s) 145, 231, 301, 525, 729, 979, 1064, 1632
MflI RGATCY 3 cut(s) 145, 231, 979
MhlI GDGCHC 2 cut(s) 403, 1049
MluCI AATT 5 cut(s) 246, 489, 1191, 1296, 1390
MlyI GAGTC 4 cut(s) 202, 842, 1360, 1656
MmeI TCCRAC 3 cut(s) 355, 561, 1078
MroI TCCGGA 1 cut(s) 936
MseI TTAA 1 cut(s) 1005
MslI CAYNNNNRTG 1 cut(s) 699
MspA1I CMGCKG 3 cut(s) 971, 1132, 1397
MspI CCGG 2 cut(s) 937, 1686
MspR9I CCNGG 4 cut(s) 1248, 1420, 1620, 1687
MvaI CCWGG 3 cut(s) 1248, 1420, 1620
MwoI GCNNNNNNNGC 4 cut(s) 389, 398, 427, 836
NciI CCSGG 1 cut(s) 1687
NcoI CCATGG 1 cut(s) 1317
NdeI CATATG 1 cut(s) 700
NdeII GATC 8 cut(s) 145, 231, 301, 525, 729, 979, 1064, 1632
NlaIII CATG 5 cut(s) 487, 759, 1142, 1223, 1321
NlaIV GGNNCC 1 cut(s) 67
NmeAIII GCCGAG 1 cut(s) 364
NmuCI GTSAC 3 cut(s) 440, 1601, 1676
NspI RCATGY 1 cut(s) 1223
PaeR7I CTCGAG 1 cut(s) 513
PagI TCATGA 1 cut(s) 1138
PcsI WCGNNNNNNNCGW 1 cut(s) 1069
PfeI GAWTC 6 cut(s) 128, 275, 327, 624, 1118, 1277
PflMI CCANNNNNTGG 2 cut(s) 1097, 1625
PfoI TCCNGGA 2 cut(s) 1246, 1418
PkrI GCNGC 3 cut(s) 346, 364, 576
PleI GAGTC 4 cut(s) 202, 841, 1360, 1656
PpsI GAGTC 4 cut(s) 202, 841, 1360, 1656
PpuMI RGGWCCY 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 1049
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 3 cut(s) 1246, 1418, 1618
PspEI GGTNACC 1 cut(s) 440
PspFI CCCAGC 1 cut(s) 1397
PspGI CCWGG 3 cut(s) 1246, 1418, 1618
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 2 cut(s) 221, 1250
PspPPI RGGWCCY 1 cut(s) 221
PspXI VCTCGAGB 1 cut(s) 513
PstI CTGCAG 1 cut(s) 1150
PsuI RGATCY 3 cut(s) 145, 231, 979
RsaI GTAC 6 cut(s) 105, 693, 897, 1026, 1218, 1239
RsaNI GTAC 6 cut(s) 104, 692, 896, 1025, 1217, 1238
RseI CAYNNNNRTG 1 cut(s) 699
SacI GAGCTC 1 cut(s) 1049
SaqAI TTAA 1 cut(s) 1005
SatI GCNGC 3 cut(s) 345, 363, 575
Sau3AI GATC 8 cut(s) 145, 231, 301, 525, 729, 979, 1064, 1632
Sau96I GGNCC 2 cut(s) 221, 1250
SchI GAGTC 4 cut(s) 202, 842, 1360, 1656
ScrFI CCNGG 4 cut(s) 1248, 1420, 1620, 1687
SduI GDGCHC 2 cut(s) 403, 1049
SfaNI GCATC 3 cut(s) 307, 601, 1480
SfcI CTRYAG 3 cut(s) 606, 1035, 1146
Sfr274I CTCGAG 1 cut(s) 513
SinI GGWCC 2 cut(s) 221, 1250
SlaI CTCGAG 1 cut(s) 513
SmiMI CAYNNNNRTG 1 cut(s) 699
SmlI CTYRAG 3 cut(s) 513, 1015, 1226
SmoI CTYRAG 3 cut(s) 513, 1015, 1226
Sse9I AATT 5 cut(s) 246, 489, 1191, 1296, 1390
SsiI CCGC 5 cut(s) 969, 1130, 1395, 1562, 1753
SspMI CTAG 3 cut(s) 60, 1440, 1482
SstI GAGCTC 1 cut(s) 1049
StyD4I CCNGG 4 cut(s) 1246, 1418, 1618, 1685
StyI CCWWGG 2 cut(s) 619, 1317
TaaI ACNGT 2 cut(s) 181, 1591
TaiI ACGT 3 cut(s) 353, 1012, 1603
TaqI TCGA 5 cut(s) 514, 825, 956, 975, 1083
TasI AATT 5 cut(s) 246, 489, 1191, 1296, 1390
TatI WGTACW 3 cut(s) 691, 895, 1237
TfiI GAWTC 6 cut(s) 128, 275, 327, 624, 1118, 1277
Tru1I TTAA 1 cut(s) 1005
Tru9I TTAA 1 cut(s) 1005
TscAI CASTG 2 cut(s) 1290, 1594
TseFI GTSAC 3 cut(s) 440, 1601, 1676
TseI GCWGC 3 cut(s) 344, 362, 574
Tsp45I GTSAC 3 cut(s) 440, 1601, 1676
TspDTI ATGAA 8 cut(s) 258, 297, 660, 1127, 1155, 1188, 1281, 1323
TspGWI ACGGA 2 cut(s) 425, 848
TspRI CASTG 2 cut(s) 1290, 1594
Van91I CCANNNNNTGG 2 cut(s) 1097, 1625
VpaK11BI GGWCC 2 cut(s) 221, 1250
XapI RAATTY 2 cut(s) 1191, 1390
XceI RCATGY 1 cut(s) 1223
XhoI CTCGAG 1 cut(s) 513
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 3 cut(s) 60, 1440, 1482
ZraI GACGTC 1 cut(s) 1601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.