Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005039

Physical Location & Seq

Genomic Coordinates Forward (+)

28676610 .. 28680887

Gene Structure 4278 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1196500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1770 bp

ATGGGCAAATCACCGGCCAAATGGATCAAAACGGTGCTGTTTGGAAAGAAATCTTCGAAAGCTGGCATTCCTAAAGGAAGAGAGAAAATTACAAATCAGAAAGAGGTGGTGATTGCTGCCAGGGCAACCGGAGCCGATTATTCTTCAGATCCACCTGTGGCTTTTCATGAAACCACCAACACCATAGATAACAACCGAGACTTAGAATTGGAGAACAAGGAAACTCCAAGTGTATTAAGTGATGGAGGTCCAGGAATTCAAAGTACAGAAACACAAGGATCCGCGCCACCACAAGATGCGGCGCATGATCCTGAGAGAATCAGGCAAGAGCAAGCTGCAACAAAGGCGCAGGCTGCCTTTAGGGGTTATCTAGCACGCCGAGCATTTTGGGCCCTCAAAGGAATAATAAGGTTACAGGCACTTATCCGGGGGCACCTGGTTCGGAGACAAGCTGTTTCTACTCTGTTCAGTGTGGCGGGCATTGTCAAGTGTCAGGCACTTGCTCGTGGAAAACGGGTGAGACAGTCTGATGTTGGGCTGGAAGTGCAAAAGATATGCAGTGTGATGCCTCTGGAGGGCAAGCTAGTAAATCCTGTTGGAGTTAATACGTCAGTTCAAATGGCAAAGCTGTCAGCAAATGTTTTCATTCTCAAGCTTCTTGCTTTCTCACCTACTGTGATGCCTCTGAGTTTGCAATATGAACCCGGGAATCCAAATTCAGTTTCAAACTGGTTGGAACGATGGTCAGCAACCCATTTTTGGAAACCAGTTCCCCAACCAAGGAAAGTTCTAGATTCAAAATCTCAGAGAAAGCACCCGGAGGCTCAAACAGGCAGGGTAAAGCGCAACAGCCGGAGGCTTTCTTCTGCAAATGTTGAAAGTGTCTCAGTGCAAGCAACCTCTGAATTTGAGAAACCCAAGCGTAACCTTAGGAAAGTTCCTAGCCATCCTACAGATGCAGTGCAGGAAAACCCACAAGTTGAGCTTGAAAAGGTTAAGCGTAATTTGAGGAAGGTTCATAGTCCCATTGTAGAGAACTCTGTGCAGGCAGAAAGTGAAGCTGAGGGTATCAAACAGAGTTTGGAAAAGGCATCAAGCACTTTGGTTCCTGATGTTCTTGAAGGCACCGGTAACTCTGTGGGGAAGTTTAAGAAAGAGCTAACCTGGATTTCATCCAGTCGGCCTGATACAGAAATAACTCCTGAACCATCGGCACCAAAAGAGGTATTTAACCTATCATCTATTGATCAAGCTGCAGAAGATCTGAAGCCTTTGACGGATAGCACTAGTAAAGATGTGAATACACCTAGCGCTGAAGCTCCAATTGAGTCGAAGATTTTAACTGAGAGCAATGGTCAGGATGAAAATATCTCATCCTCAAACGGGGTTTTGAGCCACAAGGAAGATTTAACAACCAATGACAACCAGAAATCTATCAGGAAAGTTTCTACTCCAGCAAAACAAGAACGTTCGGAGAATGGGTTGCAAAGCAGTCCAACAGTACCAAGTTATATGGCTGCAACTGAATCTGCAAAGGCAAAGCTGAGAGCACAAGGCTCCCCTAGGTTGGCACAAGACGTGACTGAGAAAAATAACTCTACTCGTCGTCATTCTCTGCCATCAGCAACTAATACCAACATCAGCTCACAATCGCCAAGGATGCATCGACTTGTTCAAACAGGTGGCAAAGGAGGAAACAAAAGTGACAAACCTCGGGCAACTTCGAGAGATGGAATTGGAAAGGTAACCCAAACAGAGTGGAGGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005875 GO:0005886 GO:0015630 GO:0016020 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4005 IQ

Protein Analysis

590

Amino Acids

64.37

Weight (kDa)

9.85

Isoelectric Point (pI)

49.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF4005	PF13178	477 - 568	6e-15	Protein of unknown function (DUF4005)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005039)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18840 AT1G18840 AT1G18840 AT1G74690
fragaria_vesca	FvH4_5g15290 FvH4_5g15290 FvH4_5g15290 FvH4_5g15290
malus_domestica	MD06G1189500.v1.1 MD14G1196500.v1.1
prunus_persica	Prupe.5G188300_v2.0.a1
pyrus_communis	pycom06g16840 pycom14g16320
rosa_chinensis	RchiOBHm_Chr7g0180771
rosa_laevigata	RLG00000005231
rosa_multiflora	Rmu_co8363381.1_g000001 Rmu_co8401147.1_g000001 Rmu_co8418241.1_g000001 Rmu_sc0007457.1_g000011 Rmu_sc0026332.1_g000002 Rmu_sc0030245.1_g000001
rosa_roxburghii	Rroxscaffold_3G00226750 Rroxscaffold_3G00272540
rosa_rugosa	Rorug06G0435900
rosa_samantha	Rh1AG249900 Rh7AG037600 Rh7BG037300 Rh7CG039000 Rh7DG037500
rosa_wichuraiana	Rw7G003100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	432, 1124, 1213
AccII	CGCG	1 cut(s)	284
AciI	CCGC	3 cut(s)	282, 299, 476
AclI	AACGTT	1 cut(s)	1468
AclWI	GGATC	5 cut(s)	32, 143, 273, 286, 302
AcoI	YGGCCR	1 cut(s)	15
AcsI	RAATTY	3 cut(s)	255, 715, 905
AcuI	CTGAAG	3 cut(s)	129, 1286, 1335
AfaI	GTAC	2 cut(s)	265, 1503
AfeI	AGCGCT	1 cut(s)	1312
AfiI	CCNNNNNNNGG	5 cut(s)	575, 759, 780, 1383, 1567
AgeI	ACCGGT	1 cut(s)	1127
AgsI	TTSAA	8 cut(s)	260, 617, 726, 798, 878, 989, 1121, 1676
AhlI	ACTAGT	1 cut(s)	1286
AjiI	CACGTC	1 cut(s)	1579
AjnI	CCWGG	4 cut(s)	119, 250, 435, 1163
AjuI	GAANNNNNNNTTGG	2 cut(s)	706, 738
Alw21I	GWGCWC	1 cut(s)	1552
Alw26I	GTCTC	4 cut(s)	192, 439, 514, 889
AlwI	GGATC	5 cut(s)	32, 143, 273, 286, 302
Ama87I	CYCGRG	2 cut(s)	704, 1713
Aor51HI	AGCGCT	1 cut(s)	1312
AoxI	GGCC	3 cut(s)	15, 390, 1181
ApaI	GGGCCC	1 cut(s)	394
ApeKI	GCWGC	5 cut(s)	116, 335, 353, 1253, 1517
ApoI	RAATTY	3 cut(s)	255, 715, 905
AsiGI	ACCGGT	1 cut(s)	1127
AspA2I	CCTAGG	1 cut(s)	1562
AspLEI	GCGC	5 cut(s)	286, 304, 349, 846, 1313
AspS9I	GGNCC	3 cut(s)	248, 390, 391
AsuC2I	CCSGG	4 cut(s)	428, 705, 706, 818
AsuHPI	GGTGA	4 cut(s)	3, 121, 529, 660
AsuII	TTCGAA	1 cut(s)	56
AvaI	CYCGRG	2 cut(s)	704, 1713
AvaII	GGWCC	1 cut(s)	248
AvrII	CCTAGG	1 cut(s)	1562
AxyI	CCTNAGG	1 cut(s)	929
BaeGI	GKGCMC	2 cut(s)	394, 435
BamHI	GGATCC	1 cut(s)	278
BanI	GGYRCC	3 cut(s)	432, 1124, 1213
BanII	GRGCYC	1 cut(s)	394
BauI	CACGAG	1 cut(s)	504
Bbv12I	GWGCWC	1 cut(s)	1552
BbvCI	CCTCAGC	1 cut(s)	1062
BbvI	GCAGC	5 cut(s)	103, 322, 340, 1240, 1504
BccI	CCATC	6 cut(s)	236, 735, 954, 1216, 1627, 1724
BcgI	CGANNNNNNTGC	2 cut(s)	422, 456
BciT130I	CCWGG	4 cut(s)	121, 252, 437, 1165
BclI	TGATCA	1 cut(s)	1246
BcnI	CCSGG	4 cut(s)	428, 705, 706, 818
BcoDI	GTCTC	4 cut(s)	192, 439, 514, 889
BcuI	ACTAGT	1 cut(s)	1286
BfaI	CTAG	7 cut(s)	371, 584, 791, 942, 1287, 1308, 1563
BfmI	CTRYAG	2 cut(s)	951, 1254
BfoI	RGCGCY	1 cut(s)	1314
BglII	AGATCT	1 cut(s)	1261
BisI	GCNGC	6 cut(s)	117, 300, 336, 354, 1254, 1518
BlnI	CCTAGG	1 cut(s)	1562
BlsI	GCNGC	6 cut(s)	118, 301, 337, 355, 1255, 1519
Bme1390I	CCNGG	8 cut(s)	121, 252, 428, 437, 705, 706, 818, 1165
Bme18I	GGWCC	1 cut(s)	248
BmeT110I	CYCGRG	2 cut(s)	704, 1713
BmgBI	CACGTC	1 cut(s)	1579
BmgT120I	GGNCC	3 cut(s)	248, 390, 391
BmiI	GGNNCC	8 cut(s)	133, 280, 392, 434, 1107, 1126, 1215, 1558
BmrFI	CCNGG	8 cut(s)	121, 252, 428, 437, 705, 706, 818, 1165
BmsI	GCATC	7 cut(s)	286, 555, 669, 946, 1100, 1650, 1672
BpmI	CTGGAG	2 cut(s)	593, 1437
Bpu10I	CCTNAGC	1 cut(s)	1062
Bpu14I	TTCGAA	1 cut(s)	56
BpuEI	CTTGAG	1 cut(s)	635
BpuMI	CCSGG	4 cut(s)	428, 705, 706, 818
BsaJI	CCNNGG	7 cut(s)	120, 427, 704, 779, 1562, 1655, 1712
BsaWI	WCCGGW	2 cut(s)	128, 1127
BsaXI	ACNNNNNCTCC	2 cut(s)	1466, 1496
Bsc4I	CCNNNNNNNGG	5 cut(s)	575, 759, 780, 1383, 1567
Bse118I	RCCGGY	2 cut(s)	13, 1127
Bse1I	ACTGG	3 cut(s)	734, 767, 1176
Bse21I	CCTNAGG	1 cut(s)	929
Bse3DI	GCAATG	1 cut(s)	1357
BseBI	CCWGG	4 cut(s)	121, 252, 437, 1165
BseDI	CCNNGG	7 cut(s)	120, 427, 704, 779, 1562, 1655, 1712
BseGI	GGATG	5 cut(s)	946, 1172, 1366, 1373, 1665
BseLI	CCNNNNNNNGG	5 cut(s)	575, 759, 780, 1383, 1567
BseMI	GCAATG	1 cut(s)	1357
BseMII	CTCAG	8 cut(s)	303, 677, 818, 900, 1053, 1335, 1535, 1575
BseNI	ACTGG	3 cut(s)	734, 767, 1176
BseSI	GKGCMC	2 cut(s)	394, 435
BseXI	GCAGC	5 cut(s)	103, 322, 340, 1240, 1504
BsgI	GTGCAG	2 cut(s)	983, 1064
Bsh1236I	CGCG	1 cut(s)	284
BshFI	GGCC	3 cut(s)	17, 392, 1183
BshNI	GGYRCC	3 cut(s)	432, 1124, 1213
BshTI	ACCGGT	1 cut(s)	1127
BsiHKAI	GWGCWC	1 cut(s)	1552
BsiHKCI	CYCGRG	2 cut(s)	704, 1713
BsiSI	CCGG	7 cut(s)	14, 129, 427, 705, 818, 853, 1128
BslFI	GGGAC	1 cut(s)	1008
BslI	CCNNNNNNNGG	5 cut(s)	575, 759, 780, 1383, 1567
BsmAI	GTCTC	4 cut(s)	192, 439, 514, 889
BsmFI	GGGAC	1 cut(s)	1008
BsmI	GAATGC	1 cut(s)	66
BsnI	GGCC	3 cut(s)	17, 392, 1183
BsoBI	CYCGRG	2 cut(s)	704, 1713
Bsp119I	TTCGAA	1 cut(s)	56
Bsp120I	GGGCCC	1 cut(s)	390
Bsp1286I	GDGCHC	3 cut(s)	394, 435, 1552
Bsp143I	GATC	6 cut(s)	24, 148, 278, 307, 1246, 1261
BspACI	CCGC	3 cut(s)	282, 299, 476
BspANI	GGCC	3 cut(s)	17, 392, 1183
BspCNI	CTCAG	8 cut(s)	304, 678, 817, 899, 1054, 1336, 1536, 1576
BspFNI	CGCG	1 cut(s)	284
BspHI	TCATGA	1 cut(s)	166
BspLI	GGNNCC	8 cut(s)	133, 280, 392, 434, 1107, 1126, 1215, 1558
BspMAI	CTGCAG	1 cut(s)	1258
BspPI	GGATC	5 cut(s)	32, 143, 273, 286, 302
BspT104I	TTCGAA	1 cut(s)	56
BspT107I	GGYRCC	3 cut(s)	432, 1124, 1213
BsrDI	GCAATG	1 cut(s)	1357
BsrFI	RCCGGY	2 cut(s)	13, 1127
BsrI	ACTGG	3 cut(s)	734, 767, 1176
BssAI	RCCGGY	2 cut(s)	13, 1127
BssECI	CCNNGG	7 cut(s)	120, 427, 704, 779, 1562, 1655, 1712
BssMI	GATC	6 cut(s)	24, 148, 278, 307, 1246, 1261
BssSI	CACGAG	1 cut(s)	504
BssT1I	CCWWGG	3 cut(s)	779, 1562, 1655
Bst2BI	CACGAG	1 cut(s)	504
Bst2UI	CCWGG	4 cut(s)	121, 252, 437, 1165
Bst4CI	ACNGT	4 cut(s)	34, 525, 676, 1501
Bst6I	CTCTTC	1 cut(s)	73
BstBI	TTCGAA	1 cut(s)	56
BstC8I	GCNNGC	8 cut(s)	64, 333, 351, 376, 478, 581, 894, 1047
BstEII	GGTNACC	1 cut(s)	1744
BstF5I	GGATG	5 cut(s)	946, 1172, 1366, 1373, 1665
BstFNI	CGCG	1 cut(s)	284
BstH2I	RGCGCY	1 cut(s)	1314
BstHHI	GCGC	5 cut(s)	286, 304, 349, 846, 1313
BstKTI	GATC	6 cut(s)	27, 151, 281, 310, 1249, 1264
BstMAI	GTCTC	4 cut(s)	192, 439, 514, 889
BstMBI	GATC	6 cut(s)	24, 148, 278, 307, 1246, 1261
BstMWI	GCNNNNNNNGC	8 cut(s)	122, 131, 344, 353, 380, 389, 544, 1660
BstNI	CCWGG	4 cut(s)	121, 252, 437, 1165
BstPI	GGTNACC	1 cut(s)	1744
BstSCI	CCNGG	8 cut(s)	119, 250, 426, 435, 703, 704, 816, 1163
BstSFI	CTRYAG	2 cut(s)	951, 1254
BstSLI	GKGCMC	2 cut(s)	394, 435
BstUI	CGCG	1 cut(s)	284
BstV1I	GCAGC	5 cut(s)	103, 322, 340, 1240, 1504
BstX2I	RGATCY	3 cut(s)	148, 278, 1261
BstYI	RGATCY	3 cut(s)	148, 278, 1261
Bsu36I	CCTNAGG	1 cut(s)	929
BsuRI	GGCC	3 cut(s)	17, 392, 1183
BtrI	CACGTC	1 cut(s)	1579
BtsCI	GGATG	5 cut(s)	946, 1172, 1366, 1373, 1665
BtsI	GCAGTG	2 cut(s)	565, 966
BtsIMutI	CAGTG	4 cut(s)	475, 565, 894, 966
Cac8I	GCNNGC	8 cut(s)	64, 333, 351, 376, 478, 581, 894, 1047
CciI	TCATGA	1 cut(s)	166
CfoI	GCGC	5 cut(s)	286, 304, 349, 846, 1313
Cfr10I	RCCGGY	2 cut(s)	13, 1127
Cfr13I	GGNCC	3 cut(s)	248, 390, 391
Cfr9I	CCCGGG	1 cut(s)	704
CsiI	ACCWGGT	1 cut(s)	435
Csp6I	GTAC	2 cut(s)	264, 1502
CspAI	ACCGGT	1 cut(s)	1127
CspCI	CAANNNNNGTGG	1 cut(s)	1739
CviAII	CATG	2 cut(s)	167, 305
CviQI	GTAC	2 cut(s)	264, 1502
DpnI	GATC	6 cut(s)	26, 150, 280, 309, 1248, 1263
DpnII	GATC	6 cut(s)	24, 148, 278, 307, 1246, 1261
EaeI	YGGCCR	1 cut(s)	15
Eam1104I	CTCTTC	1 cut(s)	73
EarI	CTCTTC	1 cut(s)	73
Eco130I	CCWWGG	3 cut(s)	779, 1562, 1655
Eco24I	GRGCYC	1 cut(s)	394
Eco47I	GGWCC	1 cut(s)	248
Eco47III	AGCGCT	1 cut(s)	1312
Eco57I	CTGAAG	3 cut(s)	129, 1286, 1335
Eco81I	CCTNAGG	1 cut(s)	929
Eco88I	CYCGRG	2 cut(s)	704, 1713
Eco91I	GGTNACC	1 cut(s)	1744
EcoO109I	RGGNCCY	1 cut(s)	391
EcoO65I	GGTNACC	1 cut(s)	1744
EcoRI	GAATTC	1 cut(s)	255
EcoRII	CCWGG	4 cut(s)	119, 250, 435, 1163
EcoT14I	CCWWGG	3 cut(s)	779, 1562, 1655
EcoT22I	ATGCAT	1 cut(s)	1665
EcoT38I	GRGCYC	1 cut(s)	394
ErhI	CCWWGG	3 cut(s)	779, 1562, 1655
FaeI	CATG	2 cut(s)	170, 308
FaiI	YATR	8 cut(s)	168, 185, 306, 556, 699, 1020, 1512, 1514
FalI	AAGNNNNNCTT	2 cut(s)	969, 1001
FaqI	GGGAC	1 cut(s)	1008
FatI	CATG	2 cut(s)	166, 304
FauI	CCCGC	1 cut(s)	469
FbaI	TGATCA	1 cut(s)	1246
Fnu4HI	GCNGC	6 cut(s)	117, 300, 336, 354, 1254, 1518
FokI	GGATG	5 cut(s)	933, 1159, 1360, 1373, 1672
FriOI	GRGCYC	1 cut(s)	394
Fsp4HI	GCNGC	6 cut(s)	117, 300, 336, 354, 1254, 1518
FspBI	CTAG	7 cut(s)	371, 584, 791, 942, 1287, 1308, 1563
GlaI	GCGC	5 cut(s)	285, 303, 348, 845, 1312
GluI	GCNGC	6 cut(s)	117, 300, 336, 354, 1254, 1518
GsuI	CTGGAG	2 cut(s)	593, 1437
HaeII	RGCGCY	1 cut(s)	1314
HaeIII	GGCC	3 cut(s)	17, 392, 1183
HapII	CCGG	7 cut(s)	14, 129, 427, 705, 818, 853, 1128
HhaI	GCGC	5 cut(s)	286, 304, 349, 846, 1313
Hin1II	CATG	2 cut(s)	170, 308
Hin6I	GCGC	5 cut(s)	284, 302, 347, 844, 1311
HinP1I	GCGC	5 cut(s)	284, 302, 347, 844, 1311
HindIII	AAGCTT	1 cut(s)	653
HinfI	GANTC	5 cut(s)	318, 709, 794, 1328, 1526
HpaII	CCGG	7 cut(s)	14, 129, 427, 705, 818, 853, 1128
HphI	GGTGA	4 cut(s)	3, 121, 529, 660
Hpy188I	TCNGA	9 cut(s)	99, 148, 444, 529, 687, 807, 904, 1266, 1474
Hpy99I	CGWCG	1 cut(s)	1608
HpyAV	CCTTC	2 cut(s)	1006, 1115
HpyCH4III	ACNGT	4 cut(s)	34, 525, 676, 1501
HpyCH4IV	ACGT	3 cut(s)	608, 1468, 1578
HpyF10VI	GCNNNNNNNGC	8 cut(s)	122, 131, 344, 353, 380, 389, 544, 1660
HpySE526I	ACGT	3 cut(s)	608, 1468, 1578
Hsp92II	CATG	2 cut(s)	170, 308
HspAI	GCGC	5 cut(s)	284, 302, 347, 844, 1311
Ksp22I	TGATCA	1 cut(s)	1246
Kzo9I	GATC	6 cut(s)	24, 148, 278, 307, 1246, 1261
LmnI	GCTCC	3 cut(s)	131, 1324, 1562
Lsp1109I	GCAGC	5 cut(s)	103, 322, 340, 1240, 1504
LweI	GCATC	7 cut(s)	286, 555, 669, 946, 1100, 1650, 1672
MabI	ACCWGGT	1 cut(s)	435
MaeI	CTAG	7 cut(s)	371, 584, 791, 942, 1287, 1308, 1563
MaeII	ACGT	3 cut(s)	608, 1468, 1578
MaeIII	GTNAC	6 cut(s)	411, 923, 1130, 1579, 1703, 1744
MalI	GATC	6 cut(s)	26, 150, 280, 309, 1248, 1263
MboI	GATC	6 cut(s)	24, 148, 278, 307, 1246, 1261
MboII	GAAGA	7 cut(s)	45, 90, 135, 855, 1271, 1345, 1415
MfeI	CAATTG	1 cut(s)	1323
MflI	RGATCY	3 cut(s)	148, 278, 1261
MhlI	GDGCHC	3 cut(s)	394, 435, 1552
MluCI	AATT	8 cut(s)	87, 206, 255, 715, 905, 1003, 1323, 1734
MlyI	GAGTC	1 cut(s)	1337
MmeI	TCCRAC	3 cut(s)	577, 714, 1520
Mph1103I	ATGCAT	1 cut(s)	1665
MseI	TTAA	7 cut(s)	236, 603, 996, 1149, 1230, 1340, 1409
MspI	CCGG	7 cut(s)	14, 129, 427, 705, 818, 853, 1128
MspR9I	CCNGG	8 cut(s)	121, 252, 428, 437, 705, 706, 818, 1165
MunI	CAATTG	1 cut(s)	1323
Mva1269I	GAATGC	1 cut(s)	66
MvaI	CCWGG	4 cut(s)	121, 252, 437, 1165
MvnI	CGCG	1 cut(s)	284
MwoI	GCNNNNNNNGC	8 cut(s)	122, 131, 344, 353, 380, 389, 544, 1660
NciI	CCSGG	4 cut(s)	428, 705, 706, 818
NdeII	GATC	6 cut(s)	24, 148, 278, 307, 1246, 1261
NlaIII	CATG	2 cut(s)	170, 308
NlaIV	GGNNCC	8 cut(s)	133, 280, 392, 434, 1107, 1126, 1215, 1558
NmeAIII	GCCGAG	1 cut(s)	404
NmuCI	GTSAC	2 cut(s)	1579, 1703
NsiI	ATGCAT	1 cut(s)	1665
NspV	TTCGAA	1 cut(s)	56
PagI	TCATGA	1 cut(s)	166
PctI	GAATGC	1 cut(s)	66
PfeI	GAWTC	4 cut(s)	318, 709, 794, 1526
PfoI	TCCNGGA	1 cut(s)	250
PinAI	ACCGGT	1 cut(s)	1127
PkrI	GCNGC	6 cut(s)	118, 301, 337, 355, 1255, 1519
PleI	GAGTC	1 cut(s)	1336
PpsI	GAGTC	1 cut(s)	1336
Psp1406I	AACGTT	1 cut(s)	1468
Psp6I	CCWGG	4 cut(s)	119, 250, 435, 1163
PspEI	GGTNACC	1 cut(s)	1744
PspGI	CCWGG	4 cut(s)	119, 250, 435, 1163
PspN4I	GGNNCC	8 cut(s)	133, 280, 392, 434, 1107, 1126, 1215, 1558
PspOMI	GGGCCC	1 cut(s)	390
PspPI	GGNCC	3 cut(s)	248, 390, 391
PstI	CTGCAG	1 cut(s)	1258
PsuI	RGATCY	3 cut(s)	148, 278, 1261
RsaI	GTAC	2 cut(s)	265, 1503
RsaNI	GTAC	2 cut(s)	264, 1502
SaqAI	TTAA	7 cut(s)	236, 603, 996, 1149, 1230, 1340, 1409
SatI	GCNGC	6 cut(s)	117, 300, 336, 354, 1254, 1518
Sau3AI	GATC	6 cut(s)	24, 148, 278, 307, 1246, 1261
Sau96I	GGNCC	3 cut(s)	248, 390, 391
SchI	GAGTC	1 cut(s)	1337
ScrFI	CCNGG	8 cut(s)	121, 252, 428, 437, 705, 706, 818, 1165
SduI	GDGCHC	3 cut(s)	394, 435, 1552
SexAI	ACCWGGT	1 cut(s)	435
SfaNI	GCATC	7 cut(s)	286, 555, 669, 946, 1100, 1650, 1672
SfcI	CTRYAG	2 cut(s)	951, 1254
SfuI	TTCGAA	1 cut(s)	56
SinI	GGWCC	1 cut(s)	248
SmaI	CCCGGG	1 cut(s)	706
SmlI	CTYRAG	1 cut(s)	650
SmoI	CTYRAG	1 cut(s)	650
SpeI	ACTAGT	1 cut(s)	1286
Sse9I	AATT	8 cut(s)	87, 206, 255, 715, 905, 1003, 1323, 1734
SsiI	CCGC	3 cut(s)	282, 299, 476
SspMI	CTAG	7 cut(s)	371, 584, 791, 942, 1287, 1308, 1563
StyD4I	CCNGG	8 cut(s)	119, 250, 426, 435, 703, 704, 816, 1163
StyI	CCWWGG	3 cut(s)	779, 1562, 1655
TaaI	ACNGT	4 cut(s)	34, 525, 676, 1501
TaiI	ACGT	3 cut(s)	611, 1471, 1581
TaqI	TCGA	4 cut(s)	56, 1331, 1666, 1724
TasI	AATT	8 cut(s)	87, 206, 255, 715, 905, 1003, 1323, 1734
TatI	WGTACW	1 cut(s)	263
TauI	GCSGC	1 cut(s)	302
TfiI	GAWTC	4 cut(s)	318, 709, 794, 1526
Tru1I	TTAA	7 cut(s)	236, 603, 996, 1149, 1230, 1340, 1409
Tru9I	TTAA	7 cut(s)	236, 603, 996, 1149, 1230, 1340, 1409
TscAI	CASTG	4 cut(s)	475, 565, 894, 966
TseFI	GTSAC	2 cut(s)	1579, 1703
TseI	GCWGC	5 cut(s)	116, 335, 353, 1253, 1517
Tsp45I	GTSAC	2 cut(s)	1579, 1703
TspDTI	ATGAA	7 cut(s)	155, 183, 634, 714, 1007, 1161, 1377
TspGWI	ACGGA	1 cut(s)	1292
TspMI	CCCGGG	1 cut(s)	704
TspRI	CASTG	4 cut(s)	475, 565, 894, 966
VpaK11BI	GGWCC	1 cut(s)	248
XapI	RAATTY	3 cut(s)	255, 715, 905
XbaI	TCTAGA	1 cut(s)	790
XmaI	CCCGGG	1 cut(s)	704
XmaJI	CCTAGG	1 cut(s)	1562
XspI	CTAG	7 cut(s)	371, 584, 791, 942, 1287, 1308, 1563
Zsp2I	ATGCAT	1 cut(s)	1665

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.