MD06G1189500.v1.1

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005039
Physical Location & Seq
Forward (+)
32626887 .. 32630971
4085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1189500.v1.1.491

Sequence Viewer

Length: 1773 bp
ATGGGCAAATCACCGGCAAAATGGATCAAAACGGTGCTCTTTGGAAAGAAATCTTCAAAAGGTGGCATTCCTAAAGGAAGAGAGAAAATTTCAAATGAGAAAGAGGTGGTGGTGGTTGCTGCCAGGGCAACCGGAGCTGATTATTCTTCAGATCCACCTGTGGCTTTCCATGAAACCACCAACACCATAAATAACAACCGAGAATTAGAATTGGAGAACAAGGAAACTCCAAGTGTATTAAGTGATGGAAGTCCAGGAATTCAAAGTATAGAAACACAAGGATCTGCGCCACAAGATTTGGTGCCGCTTGATCCTGAGAGAATCAGGCAAGAGCAAGCTGCAACAAAGGCACAGGCTGCCTTTAGGGGTTATCTAGCACGGCGAGCATTTTGGGCCGTCAAAGGAATAATAAGGTTACAGGCACTTATCCGTGGGCACCTGGTTCGGAGACAAGCTGTTGCTACTCTGTTCAGTATGACGGGCATTGTCAAGTGTCAGGCGCTCGCTCGTGGAAAACGGGTGAGGCAGTCTGATATTGGGCTTCAAGTGCAAAACATATGCAGTGTGATGCCTCTGGAGGGCAAGTTAGTGAGTCCTGTTGGAGTTAATACGTCAGTTCAAATGGCAAAGCTGTCAGCAAATGTTTTCATTCTCAAGCTTCTTGCTTTCTCACTTACTGTGATGCCTCTGAGTTTGCAATATGAACCCGGGAATCCAAATTCAGTCTCAAACTGGTTGGAACGATGGTCCGCAACCTATTTTTGGAAACCAGTTCCCCAACCAAGGAAAGTTCCAGATTCAAAATCTCAGAGAAAGCACCCGGAGGCTCAAACAGGCAGGGTAAAGCGCAATAGCCGGAGGCTTTCTTCTGCAAATGTCGAAAGTGTCTCGGTGCAAGCAACCTCTGAATTTGAGAAACCCAAGCGTAACCTTAGAAAAGTACCTAGCCATCATACAGATCCAGTGCCGGAAAACCCACAAGTTGAGCTTGAAAAGGTTAAGCGTAATTTGAGGAAGGTTCATAGTCCCATTGTAGAGAACTCTGTGCAGGCAGAAAGTGAAGCTGAGGGTATCAAGCGGAGTTTGGAAAAGGCATCAAGCACTTTGGTTCCTGATGTTGTCGAAGGCACTAGTAACTCAGTCGAGAAGTTTAAGAAAGAGCCAACCTGGATTCCATCCGGTCGGCCTGATACAGAAATAATCCCTGAACCATGGGCACCTAAAGAGGTATTTAACATATCATCTATTGATCAAGCTGCAGAAGATCTGAAGCCTTTGACAGATAGCACTAGTAAAGATGTGAATACGCCTAGTGCTGAAGCTGCAATTGAGTCGAAGATTTTAACGGAGAGCAATGGTCAGGATGAAAATATCTCATCCTCAAACGGGGTTTTGAGCCAAAAGGAAGATTTAACAACCAATGACAACCAGAAATCTAACAGGAAAGCTTCTACTCCAGCAAAACAAGAAATTTCGGAGAATGGGTTGCAAAGCAGTCCAACAGTACCAAGTTATATGGCAGCAACTGAATCTGCAAAGGCAAAGCTTAGAGCACAAGGCTCCCCTAGGTTGGCACAAGATGTGACTGACAAAAATAACTCCACTCGCCGTCATTCTCTGTCATCAGCAACTAATGCCAAAATCAGCTCACATTCACCAAGGACACGACGACTTGTTCAAACAGGTGGCAAAGGAGGAACTAAAACCGACAGACTTCTGGCAACATCGAGTGATGGAAATGGAAAGGTAACCCAAGCAGGATGGAGAAGGTGA

Protein Analysis

591

Amino Acids

64.47

Weight (kDa)

9.85

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 475 - 564 1e-13 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 301, 435, 1216
AciI CCGC 3 cut(s) 305, 750, 1078
AclWI GGATC 5 cut(s) 32, 146, 289, 305, 953
AcsI RAATTY 5 cut(s) 87, 258, 718, 908, 1470
AcuI CTGAAG 3 cut(s) 132, 1289, 1338
AfaI GTAC 2 cut(s) 942, 1506
AfiI CCNNNNNNNGG 5 cut(s) 578, 762, 783, 1386, 1570
AgsI TTSAA 8 cut(s) 57, 93, 263, 545, 620, 801, 992, 1679
AhlI ACTAGT 2 cut(s) 1130, 1289
AjnI CCWGG 4 cut(s) 122, 253, 438, 1166
Alw21I GWGCWC 2 cut(s) 39, 1555
Alw26I GTCTC 3 cut(s) 442, 730, 892
AlwI GGATC 5 cut(s) 32, 146, 289, 305, 953
AlwNI CAGNNNCTG 1 cut(s) 1526
Ama87I CYCGRG 1 cut(s) 707
AoxI GGCC 2 cut(s) 393, 1184
ApeKI GCWGC 6 cut(s) 119, 338, 356, 1256, 1322, 1520
ApoI RAATTY 5 cut(s) 87, 258, 718, 908, 1470
AspA2I CCTAGG 1 cut(s) 1565
AspLEI GCGC 3 cut(s) 289, 502, 849
AspS9I GGNCC 2 cut(s) 393, 747
AsuC2I CCSGG 3 cut(s) 708, 709, 821
AsuHPI GGTGA 3 cut(s) 3, 532, 1647
AvaI CYCGRG 1 cut(s) 707
AvaII GGWCC 1 cut(s) 747
AvrII CCTAGG 1 cut(s) 1565
BaeGI GKGCMC 2 cut(s) 438, 1219
BanI GGYRCC 3 cut(s) 301, 435, 1216
BauI CACGAG 1 cut(s) 507
Bbv12I GWGCWC 2 cut(s) 39, 1555
BbvCI CCTCAGC 1 cut(s) 1065
BbvI GCAGC 6 cut(s) 106, 325, 343, 1243, 1309, 1532
BccI CCATC 6 cut(s) 239, 738, 957, 1183, 1727, 1755
BceAI ACGGC 3 cut(s) 380, 395, 1593
BcgI CGANNNNNNTGC 4 cut(s) 425, 459, 1314, 1348
BciT130I CCWGG 4 cut(s) 124, 255, 440, 1168
BclI TGATCA 1 cut(s) 1249
BcnI CCSGG 3 cut(s) 708, 709, 821
BcoDI GTCTC 3 cut(s) 442, 730, 892
BcuI ACTAGT 2 cut(s) 1130, 1289
BfaI CTAG 6 cut(s) 374, 945, 1131, 1290, 1311, 1566
BfmI CTRYAG 1 cut(s) 1257
BfoI RGCGCY 1 cut(s) 503
BglII AGATCT 1 cut(s) 1264
BisI GCNGC 7 cut(s) 120, 305, 339, 357, 1257, 1323, 1521
BlnI CCTAGG 1 cut(s) 1565
BlsI GCNGC 7 cut(s) 121, 306, 340, 358, 1258, 1324, 1522
Bme1390I CCNGG 7 cut(s) 124, 255, 440, 708, 709, 821, 1168
Bme18I GGWCC 1 cut(s) 747
BmeT110I CYCGRG 1 cut(s) 707
BmgT120I GGNCC 2 cut(s) 393, 747
BmiI GGNNCC 5 cut(s) 303, 437, 1110, 1218, 1561
BmrFI CCNGG 7 cut(s) 124, 255, 440, 708, 709, 821, 1168
BmsI GCATC 3 cut(s) 558, 672, 1103
BpmI CTGGAG 2 cut(s) 596, 1440
Bpu10I CCTNAGC 1 cut(s) 1065
BpuEI CTTGAG 1 cut(s) 638
BpuMI CCSGG 3 cut(s) 708, 709, 821
BsaJI CCNNGG 7 cut(s) 123, 430, 707, 782, 1211, 1565, 1658
BsaWI WCCGGW 2 cut(s) 131, 1178
BsaXI ACNNNNNCTCC 4 cut(s) 569, 599, 1469, 1499
Bsc4I CCNNNNNNNGG 5 cut(s) 578, 762, 783, 1386, 1570
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 3 cut(s) 737, 770, 962
Bse3DI GCAATG 1 cut(s) 1360
BseBI CCWGG 4 cut(s) 124, 255, 440, 1168
BseDI CCNNGG 7 cut(s) 123, 430, 707, 782, 1211, 1565, 1658
BseGI GGATG 4 cut(s) 1175, 1369, 1376, 1766
BseLI CCNNNNNNNGG 5 cut(s) 578, 762, 783, 1386, 1570
BseMI GCAATG 1 cut(s) 1360
BseMII CTCAG 5 cut(s) 306, 680, 821, 1056, 1152
BseNI ACTGG 3 cut(s) 737, 770, 962
BseSI GKGCMC 2 cut(s) 438, 1219
BseXI GCAGC 6 cut(s) 106, 325, 343, 1243, 1309, 1532
BsgI GTGCAG 1 cut(s) 1067
Bsh1285I CGRYCG 1 cut(s) 1183
BshFI GGCC 2 cut(s) 395, 1186
BshNI GGYRCC 3 cut(s) 301, 435, 1216
BsiEI CGRYCG 1 cut(s) 1183
BsiHKAI GWGCWC 2 cut(s) 39, 1555
BsiHKCI CYCGRG 1 cut(s) 707
BsiSI CCGG 7 cut(s) 14, 132, 708, 821, 856, 968, 1179
BslFI GGGAC 1 cut(s) 1011
BslI CCNNNNNNNGG 5 cut(s) 578, 762, 783, 1386, 1570
BsmAI GTCTC 3 cut(s) 442, 730, 892
BsmFI GGGAC 1 cut(s) 1011
BsmI GAATGC 1 cut(s) 66
BsnI GGCC 2 cut(s) 395, 1186
BsoBI CYCGRG 1 cut(s) 707
Bsp1286I GDGCHC 4 cut(s) 39, 438, 1219, 1555
Bsp143I GATC 7 cut(s) 24, 151, 281, 310, 958, 1249, 1264
Bsp19I CCATGG 1 cut(s) 1211
BspACI CCGC 3 cut(s) 305, 750, 1078
BspANI GGCC 2 cut(s) 395, 1186
BspCNI CTCAG 5 cut(s) 307, 681, 820, 1057, 1151
BspLI GGNNCC 5 cut(s) 303, 437, 1110, 1218, 1561
BspMAI CTGCAG 1 cut(s) 1261
BspPI GGATC 5 cut(s) 32, 146, 289, 305, 953
BspT107I GGYRCC 3 cut(s) 301, 435, 1216
BsrDI GCAATG 1 cut(s) 1360
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 3 cut(s) 737, 770, 962
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 7 cut(s) 123, 430, 707, 782, 1211, 1565, 1658
BssMI GATC 7 cut(s) 24, 151, 281, 310, 958, 1249, 1264
BssSI CACGAG 1 cut(s) 507
BssT1I CCWWGG 4 cut(s) 782, 1211, 1565, 1658
Bst2BI CACGAG 1 cut(s) 507
Bst2UI CCWGG 4 cut(s) 124, 255, 440, 1168
Bst4CI ACNGT 3 cut(s) 34, 679, 1504
Bst6I CTCTTC 1 cut(s) 73
BstAPI GCANNNNNTGC 2 cut(s) 356, 1634
BstC8I GCNNGC 5 cut(s) 336, 384, 504, 897, 1050
BstDEI CTNAG 7 cut(s) 315, 689, 807, 932, 1065, 1138, 1547
BstDSI CCRYGG 2 cut(s) 430, 1211
BstEII GGTNACC 1 cut(s) 1747
BstF5I GGATG 4 cut(s) 1175, 1369, 1376, 1766
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 3 cut(s) 289, 502, 849
BstKTI GATC 7 cut(s) 27, 154, 284, 313, 961, 1252, 1267
BstMAI GTCTC 3 cut(s) 442, 730, 892
BstMBI GATC 7 cut(s) 24, 151, 281, 310, 958, 1249, 1264
BstMCI CGRYCG 1 cut(s) 1183
BstMWI GCNNNNNNNGC 9 cut(s) 125, 134, 347, 356, 383, 392, 547, 1322, 1634
BstNI CCWGG 4 cut(s) 124, 255, 440, 1168
BstPI GGTNACC 1 cut(s) 1747
BstSCI CCNGG 7 cut(s) 122, 253, 438, 706, 707, 819, 1166
BstSFI CTRYAG 1 cut(s) 1257
BstSLI GKGCMC 2 cut(s) 438, 1219
BstV1I GCAGC 6 cut(s) 106, 325, 343, 1243, 1309, 1532
BstX2I RGATCY 4 cut(s) 151, 281, 958, 1264
BstYI RGATCY 4 cut(s) 151, 281, 958, 1264
BsuRI GGCC 2 cut(s) 395, 1186
BtgI CCRYGG 2 cut(s) 430, 1211
BtsCI GGATG 4 cut(s) 1175, 1369, 1376, 1766
BtsI GCAGTG 1 cut(s) 568
BtsIMutI CAGTG 2 cut(s) 568, 969
Cac8I GCNNGC 5 cut(s) 336, 384, 504, 897, 1050
CaiI CAGNNNCTG 1 cut(s) 1526
CfoI GCGC 3 cut(s) 289, 502, 849
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 2 cut(s) 393, 747
Cfr9I CCCGGG 1 cut(s) 707
CsiI ACCWGGT 1 cut(s) 438
Csp6I GTAC 2 cut(s) 941, 1505
CspCI CAANNNNNGTGG 2 cut(s) 279, 314
CviAII CATG 2 cut(s) 170, 1212
CviQI GTAC 2 cut(s) 941, 1505
DdeI CTNAG 7 cut(s) 315, 689, 807, 932, 1065, 1138, 1547
DpnI GATC 7 cut(s) 26, 153, 283, 312, 960, 1251, 1266
DpnII GATC 7 cut(s) 24, 151, 281, 310, 958, 1249, 1264
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Eco130I CCWWGG 4 cut(s) 782, 1211, 1565, 1658
Eco47I GGWCC 1 cut(s) 747
Eco57I CTGAAG 3 cut(s) 132, 1289, 1338
Eco88I CYCGRG 1 cut(s) 707
Eco91I GGTNACC 1 cut(s) 1747
EcoO65I GGTNACC 1 cut(s) 1747
EcoRI GAATTC 1 cut(s) 258
EcoRII CCWGG 4 cut(s) 122, 253, 438, 1166
EcoT14I CCWWGG 4 cut(s) 782, 1211, 1565, 1658
ErhI CCWWGG 4 cut(s) 782, 1211, 1565, 1658
FaeI CATG 2 cut(s) 173, 1215
FalI AAGNNNNNCTT 2 cut(s) 972, 1004
FaqI GGGAC 1 cut(s) 1011
FatI CATG 2 cut(s) 169, 1211
FauNDI CATATG 1 cut(s) 557
FbaI TGATCA 1 cut(s) 1249
Fnu4HI GCNGC 7 cut(s) 120, 305, 339, 357, 1257, 1323, 1521
FokI GGATG 3 cut(s) 1162, 1363, 1376
Fsp4HI GCNGC 7 cut(s) 120, 305, 339, 357, 1257, 1323, 1521
FspBI CTAG 6 cut(s) 374, 945, 1131, 1290, 1311, 1566
GlaI GCGC 3 cut(s) 288, 501, 848
GluI GCNGC 7 cut(s) 120, 305, 339, 357, 1257, 1323, 1521
GsuI CTGGAG 2 cut(s) 596, 1440
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 2 cut(s) 395, 1186
HapII CCGG 7 cut(s) 14, 132, 708, 821, 856, 968, 1179
HhaI GCGC 3 cut(s) 289, 502, 849
Hin1II CATG 2 cut(s) 173, 1215
Hin6I GCGC 3 cut(s) 287, 500, 847
HinP1I GCGC 3 cut(s) 287, 500, 847
HindIII AAGCTT 3 cut(s) 656, 1446, 1544
HinfI GANTC 7 cut(s) 321, 592, 712, 797, 1171, 1331, 1529
HpaII CCGG 7 cut(s) 14, 132, 708, 821, 856, 968, 1179
HphI GGTGA 3 cut(s) 3, 532, 1647
Hpy188I TCNGA 8 cut(s) 151, 447, 532, 690, 810, 907, 1269, 1477
Hpy188III TCNNGA 6 cut(s) 314, 575, 794, 1112, 1144, 1361
Hpy99I CGWCG 1 cut(s) 1671
HpyAV CCTTC 3 cut(s) 1009, 1118, 1761
HpyCH4III ACNGT 3 cut(s) 34, 679, 1504
HpyCH4IV ACGT 1 cut(s) 611
HpyF10VI GCNNNNNNNGC 9 cut(s) 125, 134, 347, 356, 383, 392, 547, 1322, 1634
HpyF3I CTNAG 7 cut(s) 315, 689, 807, 932, 1065, 1138, 1547
HpySE526I ACGT 1 cut(s) 611
Hsp92II CATG 2 cut(s) 173, 1215
HspAI GCGC 3 cut(s) 287, 500, 847
Ksp22I TGATCA 1 cut(s) 1249
Kzo9I GATC 7 cut(s) 24, 151, 281, 310, 958, 1249, 1264
LmnI GCTCC 2 cut(s) 134, 1565
Lsp1109I GCAGC 6 cut(s) 106, 325, 343, 1243, 1309, 1532
LweI GCATC 3 cut(s) 558, 672, 1103
MabI ACCWGGT 1 cut(s) 438
MaeI CTAG 6 cut(s) 374, 945, 1131, 1290, 1311, 1566
MaeII ACGT 1 cut(s) 611
MaeIII GTNAC 5 cut(s) 414, 926, 1133, 1582, 1747
MalI GATC 7 cut(s) 26, 153, 283, 312, 960, 1251, 1266
MboI GATC 7 cut(s) 24, 151, 281, 310, 958, 1249, 1264
MboII GAAGA 7 cut(s) 45, 90, 138, 858, 1274, 1348, 1418
MfeI CAATTG 1 cut(s) 1326
MflI RGATCY 4 cut(s) 151, 281, 958, 1264
MhlI GDGCHC 4 cut(s) 39, 438, 1219, 1555
MluCI AATT 9 cut(s) 87, 203, 209, 258, 718, 908, 1006, 1326, 1470
MlyI GAGTC 2 cut(s) 601, 1340
MmeI TCCRAC 3 cut(s) 580, 717, 1523
MseI TTAA 7 cut(s) 239, 606, 999, 1152, 1233, 1343, 1412
MspI CCGG 7 cut(s) 14, 132, 708, 821, 856, 968, 1179
MspR9I CCNGG 7 cut(s) 124, 255, 440, 708, 709, 821, 1168
MunI CAATTG 1 cut(s) 1326
Mva1269I GAATGC 1 cut(s) 66
MvaI CCWGG 4 cut(s) 124, 255, 440, 1168
MwoI GCNNNNNNNGC 9 cut(s) 125, 134, 347, 356, 383, 392, 547, 1322, 1634
NciI CCSGG 3 cut(s) 708, 709, 821
NcoI CCATGG 1 cut(s) 1211
NdeI CATATG 1 cut(s) 557
NdeII GATC 7 cut(s) 24, 151, 281, 310, 958, 1249, 1264
NlaIII CATG 2 cut(s) 173, 1215
NlaIV GGNNCC 5 cut(s) 303, 437, 1110, 1218, 1561
NmuCI GTSAC 1 cut(s) 1582
PctI GAATGC 1 cut(s) 66
PfeI GAWTC 5 cut(s) 321, 712, 797, 1171, 1529
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 7 cut(s) 121, 306, 340, 358, 1258, 1324, 1522
PleI GAGTC 2 cut(s) 600, 1339
PpsI GAGTC 2 cut(s) 600, 1339
Psp6I CCWGG 4 cut(s) 122, 253, 438, 1166
PspEI GGTNACC 1 cut(s) 1747
PspGI CCWGG 4 cut(s) 122, 253, 438, 1166
PspN4I GGNNCC 5 cut(s) 303, 437, 1110, 1218, 1561
PspPI GGNCC 2 cut(s) 393, 747
PstI CTGCAG 1 cut(s) 1261
PstNI CAGNNNCTG 1 cut(s) 1526
PsuI RGATCY 4 cut(s) 151, 281, 958, 1264
RsaI GTAC 2 cut(s) 942, 1506
RsaNI GTAC 2 cut(s) 941, 1505
SaqAI TTAA 7 cut(s) 239, 606, 999, 1152, 1233, 1343, 1412
SatI GCNGC 7 cut(s) 120, 305, 339, 357, 1257, 1323, 1521
Sau3AI GATC 7 cut(s) 24, 151, 281, 310, 958, 1249, 1264
Sau96I GGNCC 2 cut(s) 393, 747
SchI GAGTC 2 cut(s) 601, 1340
ScrFI CCNGG 7 cut(s) 124, 255, 440, 708, 709, 821, 1168
SduI GDGCHC 4 cut(s) 39, 438, 1219, 1555
SexAI ACCWGGT 1 cut(s) 438
SfaNI GCATC 3 cut(s) 558, 672, 1103
SfcI CTRYAG 1 cut(s) 1257
SinI GGWCC 1 cut(s) 747
SmaI CCCGGG 1 cut(s) 709
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
SpeI ACTAGT 2 cut(s) 1130, 1289
Sse9I AATT 9 cut(s) 87, 203, 209, 258, 718, 908, 1006, 1326, 1470
SsiI CCGC 3 cut(s) 305, 750, 1078
SspMI CTAG 6 cut(s) 374, 945, 1131, 1290, 1311, 1566
StyD4I CCNGG 7 cut(s) 122, 253, 438, 706, 707, 819, 1166
StyI CCWWGG 4 cut(s) 782, 1211, 1565, 1658
TaaI ACNGT 3 cut(s) 34, 679, 1504
TaiI ACGT 1 cut(s) 614
TaqI TCGA 5 cut(s) 879, 1122, 1143, 1334, 1727
TasI AATT 9 cut(s) 87, 203, 209, 258, 718, 908, 1006, 1326, 1470
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 5 cut(s) 321, 712, 797, 1171, 1529
Tru1I TTAA 7 cut(s) 239, 606, 999, 1152, 1233, 1343, 1412
Tru9I TTAA 7 cut(s) 239, 606, 999, 1152, 1233, 1343, 1412
TscAI CASTG 2 cut(s) 568, 969
TseFI GTSAC 1 cut(s) 1582
TseI GCWGC 6 cut(s) 119, 338, 356, 1256, 1322, 1520
Tsp45I GTSAC 1 cut(s) 1582
TspDTI ATGAA 5 cut(s) 186, 637, 717, 1010, 1380
TspGWI ACGGA 2 cut(s) 419, 1361
TspMI CCCGGG 1 cut(s) 707
TspRI CASTG 2 cut(s) 568, 969
VpaK11BI GGWCC 1 cut(s) 747
XapI RAATTY 5 cut(s) 87, 258, 718, 908, 1470
XmaI CCCGGG 1 cut(s) 707
XmaJI CCTAGG 1 cut(s) 1565
XspI CTAG 6 cut(s) 374, 945, 1131, 1290, 1311, 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.