Rh7AG037600

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0005039
Physical Location & Seq
Forward (+)
2560184 .. 2564641
4458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG037600.1

Sequence Viewer

Length: 1773 bp
ATGGGCAAATCACCGGCTAAATGGATCAAGACTGTTCTGTTTGGGAAGAAGCCATCCAAACCTAATATATCAAAAGGAAGAGAGAGAATTTCAAATGAGAAAGAGGTGGTGGTTGCTGCCAGGGCAGCTGCAACTGAATTTGTTGCAGATCCACCTGCAGCTTTTCTTGAAAGCACAAACACCACAGCTTATAACAAAGGGTTAGAATTGGAAAACGAGGAAGCTCCAAGTGTATTAGGTAATGGAGAGCCAGGAAGCCAAAGTACAGATTCACAAGGCTCTACACCACAAAATGCAGCGTATGATCTTGACAAAATCAGACAAGAGCAAGCTGCAACAAAGGCACAGGCTGCCTTTAGGGGTTATCTGGCTCGCCGCGCCTTTTGGGCCCTCAAAGGCATAATAAGGCTACAGGCACTTATCCGTGGGCACTTGGTGAGGAGACAAGCTGTTGCTACACTTTGCAGTATGTTGGGCATTGTCAAGCTACAGGCACTCAGTCGTGGACGACAAGTCAGGCAGTCTATTATTGGGCTTGAAGTGCAAAAAAGATGCCATGTTACGCCTCTGGAGGGCAAGCTAGTGGATCCTGTTGGAGTTAATATCTCTACTCAAATGGCAAAGCTGACGGTTAATGTTTTTATTAGTAAGCTTCTTGCTTTCTCACCTACTGTGATGCCTCTGCGTTTACATTATGAACCTGGAGATCCAAATTCAGTCCCAAACTGGTTAGAACGTTGGTCAGCAACACATTTTTGGAAACCACTTCCCCAACCAAGAAAAGTTCAAGATTCAAAATCTCAGAGAACACATCGGGATCCTCAAACAGGCAGGGTAAAGCGAAGCACCCGGAGGCTTCCGGCTGCAAATGTTGAAAGTGTCTCGGTGCAAGTTACCTCTGAATCTGAGAAACCCAAACGTATCCTTAGGAAAGTTTCCAGCCAGCCAGCAGATCCAGTGCAGGAAAATCCACAAATTGAGCTTGAAAAGGTTAAACGTAACTTGAGGAAGGTTCATACTCCCATAGTAGAGAACTCTGTGCAGACGGAAATTGAAGCTCAGAATCTCACACAGAATCTGGAAAAGGCATCAAGTGTTTCGGGTCATAATGTTTTTGAAGGGATCACAAATAATTCTGTTGAGAAGATGAAGAATGAGCCAATTGTTACTCTGTCCAATCTGCCTGATGCAGAAATAACTCCTGAACCATCAGCAGCAAAAGAGGTCTTTAACTTATTATCCGATGATCAGGTTGCAATGGATTTGAAGCCTTTGACAGAGGGCACTGATAAAGAAATAAATACACCTAGTGATGGAGCTGCAGTTGAGTCAAACATATTAACAGAGAGCAGTAAAAAAGATGAAAACATCCCTGTTCCAAGTGTGGTTTTGAGCCAAAAGGAAGATTTAACAAGCAATGACAACCAGAAATCAAGCAGGAAAGCTTCAACTCCAGCCAAACAAGAACGTTCAGAGAATGGGTTGCAAAACAGTCCAACATTGCCAAGCTATATGGCAGCAACTGAGTCTGCAAAGGCAAAGCTGAGGGCACAAGGATCTCCTAGGCTTGGACTGGAGGCTACTGATAAGACTGAGAAAACTAACTCCACTCGCCGCCATTCTCTGCCATCATCAGCTAATGGCAAAATCACTTCACAGTCTCCAAGGACACAGAAACTTGTTCAATCAGGTGGAAAAGGAGGAATCAAAGGTGACAAATCTCTGTCAGCCTCAAGAGACGGGAAGGCAAGCCAAGCAGAGTGGAGAAGATGA

Protein Analysis

590

Amino Acids

64.54

Weight (kDa)

9.77

Isoelectric Point (pI)

54.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 473 - 566 1.6e-15 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 192
AarI CACCTGC 1 cut(s) 163
Acc36I ACCTGC 1 cut(s) 163
AccII CGCG 1 cut(s) 378
AciI CCGC 2 cut(s) 376, 1615
AclI AACGTT 2 cut(s) 736, 1468
AcsI RAATTY 3 cut(s) 87, 137, 712
AdeI CACNNNGTG 2 cut(s) 436, 1310
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 4 cut(s) 572, 827, 1313, 1568
AhdI GACNNNNNGTC 1 cut(s) 512
AjnI CCWGG 3 cut(s) 119, 250, 700
Alw26I GTCTC 4 cut(s) 436, 886, 1665, 1731
AlwNI CAGNNNCTG 2 cut(s) 1078, 1523
AoxI GGCC 1 cut(s) 387
ApaI GGGCCC 1 cut(s) 391
ApoI RAATTY 3 cut(s) 87, 137, 712
AspA2I CCTAGG 1 cut(s) 1562
AspLEI GCGC 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 387, 388
AsuC2I CCSGG 1 cut(s) 850
AsuHPI GGTGA 4 cut(s) 3, 448, 657, 1724
AvrII CCTAGG 1 cut(s) 1562
AxyI CCTNAGG 1 cut(s) 926
BaeGI GKGCMC 4 cut(s) 391, 432, 1286, 1552
BamHI GGATCC 2 cut(s) 586, 817
BanII GRGCYC 1 cut(s) 391
BarI GAAGNNNNNNTAC 2 cut(s) 247, 279
BbvCI CCTCAGC 1 cut(s) 1544
BccI CCATC 4 cut(s) 61, 1216, 1307, 1636
BciT130I CCWGG 3 cut(s) 121, 252, 702
BciVI GTATCC 1 cut(s) 932
BclI TGATCA 1 cut(s) 1246
BcnI CCSGG 1 cut(s) 850
BcoDI GTCTC 4 cut(s) 436, 886, 1665, 1731
BfaI CTAG 3 cut(s) 581, 1308, 1563
BfmI CTRYAG 4 cut(s) 156, 410, 488, 1320
BfuAI ACCTGC 1 cut(s) 163
BfuI GTATCC 1 cut(s) 932
BglI GCCNNNNNGGC 1 cut(s) 386
BlnI CCTAGG 1 cut(s) 1562
Bme1390I CCNGG 4 cut(s) 121, 252, 702, 850
BmeRI GACNNNNNGTC 1 cut(s) 512
BmgT120I GGNCC 2 cut(s) 387, 388
BmiI GGNNCC 3 cut(s) 389, 588, 819
BmrFI CCNGG 4 cut(s) 121, 252, 702, 850
BmsI GCATC 4 cut(s) 542, 666, 1097, 1177
BpmI CTGGAG 4 cut(s) 590, 723, 1437, 1595
Bpu10I CCTNAGC 1 cut(s) 1544
BpuEI CTTGAG 2 cut(s) 1024, 1717
BpuMI CCSGG 1 cut(s) 850
BsaJI CCNNGG 4 cut(s) 120, 424, 1562, 1664
BsaXI ACNNNNNCTCC 2 cut(s) 1308, 1338
Bsc4I CCNNNNNNNGG 4 cut(s) 572, 827, 1313, 1568
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 3 cut(s) 731, 956, 1578
Bse21I CCTNAGG 1 cut(s) 926
Bse3DI GCAATG 3 cut(s) 1263, 1423, 1499
BseBI CCWGG 3 cut(s) 121, 252, 702
BseDI CCNNGG 4 cut(s) 120, 424, 1562, 1664
BseGI GGATG 2 cut(s) 53, 1368
BseLI CCNNNNNNNGG 4 cut(s) 572, 827, 1313, 1568
BseMI GCAATG 3 cut(s) 1263, 1423, 1499
BseMII CTCAG 7 cut(s) 511, 815, 897, 1073, 1515, 1535, 1584
BseNI ACTGG 3 cut(s) 731, 956, 1578
BseRI GAGGAG 1 cut(s) 454
BseSI GKGCMC 4 cut(s) 391, 432, 1286, 1552
BsgI GTGCAG 2 cut(s) 980, 1061
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 1 cut(s) 389
BsiSI CCGG 3 cut(s) 14, 850, 860
BslFI GGGAC 1 cut(s) 704
BslI CCNNNNNNNGG 4 cut(s) 572, 827, 1313, 1568
BsmAI GTCTC 4 cut(s) 436, 886, 1665, 1731
BsmBI CGTCTC 1 cut(s) 1731
BsmFI GGGAC 1 cut(s) 704
BsnI GGCC 1 cut(s) 389
Bsp120I GGGCCC 1 cut(s) 387
Bsp1286I GDGCHC 4 cut(s) 391, 432, 1286, 1552
BspACI CCGC 2 cut(s) 376, 1615
BspANI GGCC 1 cut(s) 389
BspCNI CTCAG 7 cut(s) 510, 814, 898, 1072, 1516, 1536, 1585
BspFNI CGCG 1 cut(s) 378
BspLI GGNNCC 3 cut(s) 389, 588, 819
BspMAI CTGCAG 2 cut(s) 160, 1324
BspMI ACCTGC 1 cut(s) 163
BsrDI GCAATG 3 cut(s) 1263, 1423, 1499
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 3 cut(s) 731, 956, 1578
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 4 cut(s) 120, 424, 1562, 1664
BssT1I CCWWGG 2 cut(s) 1562, 1664
Bst2UI CCWGG 3 cut(s) 121, 252, 702
Bst4CI ACNGT 5 cut(s) 34, 631, 673, 1493, 1659
Bst6I CTCTTC 1 cut(s) 73
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 6 cut(s) 330, 373, 578, 944, 948, 1750
BstDEI CTNAG 8 cut(s) 497, 801, 906, 926, 1059, 1524, 1544, 1593
BstDSI CCRYGG 1 cut(s) 424
BstENI CCTNNNNNAGG 1 cut(s) 825
BstF5I GGATG 2 cut(s) 53, 1368
BstFNI CGCG 1 cut(s) 378
BstHHI GCGC 1 cut(s) 380
BstMAI GTCTC 4 cut(s) 436, 886, 1665, 1731
BstMWI GCNNNNNNNGC 8 cut(s) 122, 125, 341, 350, 377, 386, 541, 1754
BstNI CCWGG 3 cut(s) 121, 252, 702
BstSCI CCNGG 4 cut(s) 119, 250, 700, 848
BstSFI CTRYAG 4 cut(s) 156, 410, 488, 1320
BstSLI GKGCMC 4 cut(s) 391, 432, 1286, 1552
BstUI CGCG 1 cut(s) 378
BstX2I RGATCY 6 cut(s) 148, 586, 706, 817, 952, 1556
BstYI RGATCY 6 cut(s) 148, 586, 706, 817, 952, 1556
Bsu36I CCTNAGG 1 cut(s) 926
BsuI GTATCC 1 cut(s) 932
BsuRI GGCC 1 cut(s) 389
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 2 cut(s) 53, 1368
BtsIMutI CAGTG 2 cut(s) 963, 1284
BveI ACCTGC 1 cut(s) 163
Cac8I GCNNGC 6 cut(s) 330, 373, 578, 944, 948, 1750
CaiI CAGNNNCTG 2 cut(s) 1078, 1523
CfoI GCGC 1 cut(s) 380
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 2 cut(s) 387, 388
Csp6I GTAC 1 cut(s) 264
CspCI CAANNNNNGTGG 1 cut(s) 1742
CviAII CATG 1 cut(s) 557
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 8 cut(s) 497, 801, 906, 926, 1059, 1524, 1544, 1593
DraIII CACNNNGTG 2 cut(s) 436, 1310
DriI GACNNNNNGTC 1 cut(s) 512
Eam1104I CTCTTC 1 cut(s) 73
Eam1105I GACNNNNNGTC 1 cut(s) 512
EarI CTCTTC 1 cut(s) 73
Eco130I CCWWGG 2 cut(s) 1562, 1664
Eco24I GRGCYC 1 cut(s) 391
Eco81I CCTNAGG 1 cut(s) 926
EcoNI CCTNNNNNAGG 1 cut(s) 825
EcoO109I RGGNCCY 1 cut(s) 388
EcoRII CCWGG 3 cut(s) 119, 250, 700
EcoT14I CCWWGG 2 cut(s) 1562, 1664
EcoT38I GRGCYC 1 cut(s) 391
ErhI CCWWGG 2 cut(s) 1562, 1664
Esp3I CGTCTC 1 cut(s) 1731
FaeI CATG 1 cut(s) 560
FaqI GGGAC 1 cut(s) 704
FatI CATG 1 cut(s) 556
FbaI TGATCA 1 cut(s) 1246
FokI GGATG 2 cut(s) 40, 1355
FriOI GRGCYC 1 cut(s) 391
FspBI CTAG 3 cut(s) 581, 1308, 1563
GlaI GCGC 1 cut(s) 379
GsuI CTGGAG 4 cut(s) 590, 723, 1437, 1595
HaeIII GGCC 1 cut(s) 389
HapII CCGG 3 cut(s) 14, 850, 860
HhaI GCGC 1 cut(s) 380
Hin1II CATG 1 cut(s) 560
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HindIII AAGCTT 2 cut(s) 650, 1443
HinfI GANTC 8 cut(s) 269, 791, 902, 1063, 1075, 1328, 1526, 1704
HpaII CCGG 3 cut(s) 14, 850, 860
HphI GGTGA 4 cut(s) 3, 448, 657, 1724
Hpy166II GTNNAC 2 cut(s) 506, 689
Hpy188I TCNGA 7 cut(s) 320, 804, 901, 907, 1062, 1243, 1474
Hpy188III TCNNGA 9 cut(s) 28, 167, 308, 569, 788, 815, 1079, 1202, 1734
Hpy8I GTNNAC 2 cut(s) 506, 689
HpyAV CCTTC 3 cut(s) 1003, 1112, 1738
HpyCH4III ACNGT 5 cut(s) 34, 631, 673, 1493, 1659
HpyCH4IV ACGT 4 cut(s) 736, 919, 997, 1468
HpyF10VI GCNNNNNNNGC 8 cut(s) 122, 125, 341, 350, 377, 386, 541, 1754
HpyF3I CTNAG 8 cut(s) 497, 801, 906, 926, 1059, 1524, 1544, 1593
HpySE526I ACGT 4 cut(s) 736, 919, 997, 1468
Hsp92II CATG 1 cut(s) 560
HspAI GCGC 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 1246
LmnI GCTCC 2 cut(s) 229, 1316
LweI GCATC 4 cut(s) 542, 666, 1097, 1177
MaeI CTAG 3 cut(s) 581, 1308, 1563
MaeII ACGT 4 cut(s) 736, 919, 997, 1468
MaeIII GTNAC 5 cut(s) 559, 892, 998, 1165, 1712
MboII GAAGA 5 cut(s) 58, 90, 1156, 1162, 1415
MfeI CAATTG 1 cut(s) 1161
MflI RGATCY 6 cut(s) 148, 586, 706, 817, 952, 1556
MhlI GDGCHC 4 cut(s) 391, 432, 1286, 1552
MluCI AATT 8 cut(s) 87, 137, 206, 712, 975, 1050, 1132, 1161
MlyI GAGTC 2 cut(s) 1337, 1535
MmeI TCCRAC 2 cut(s) 574, 1520
MseI TTAA 6 cut(s) 600, 633, 993, 1230, 1340, 1409
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 3 cut(s) 14, 850, 860
MspR9I CCNGG 4 cut(s) 121, 252, 702, 850
MunI CAATTG 1 cut(s) 1161
MvaI CCWGG 3 cut(s) 121, 252, 702
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 8 cut(s) 122, 125, 341, 350, 377, 386, 541, 1754
NciI CCSGG 1 cut(s) 850
NlaIII CATG 1 cut(s) 560
NlaIV GGNNCC 3 cut(s) 389, 588, 819
NmuCI GTSAC 1 cut(s) 1712
PaqCI CACCTGC 1 cut(s) 163
PfeI GAWTC 6 cut(s) 269, 791, 902, 1063, 1075, 1704
PleI GAGTC 2 cut(s) 1336, 1534
PpsI GAGTC 2 cut(s) 1336, 1534
PsiI TTATAA 1 cut(s) 192
Psp1406I AACGTT 2 cut(s) 736, 1468
Psp6I CCWGG 3 cut(s) 119, 250, 700
PspGI CCWGG 3 cut(s) 119, 250, 700
PspN4I GGNNCC 3 cut(s) 389, 588, 819
PspOMI GGGCCC 1 cut(s) 387
PspPI GGNCC 2 cut(s) 387, 388
PstI CTGCAG 2 cut(s) 160, 1324
PstNI CAGNNNCTG 2 cut(s) 1078, 1523
PsuI RGATCY 6 cut(s) 148, 586, 706, 817, 952, 1556
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SaqAI TTAA 6 cut(s) 600, 633, 993, 1230, 1340, 1409
Sau96I GGNCC 2 cut(s) 387, 388
SchI GAGTC 2 cut(s) 1337, 1535
ScrFI CCNGG 4 cut(s) 121, 252, 702, 850
SduI GDGCHC 4 cut(s) 391, 432, 1286, 1552
SfaNI GCATC 4 cut(s) 542, 666, 1097, 1177
SfcI CTRYAG 4 cut(s) 156, 410, 488, 1320
SmlI CTYRAG 2 cut(s) 1003, 1732
SmoI CTYRAG 2 cut(s) 1003, 1732
Sse9I AATT 8 cut(s) 87, 137, 206, 712, 975, 1050, 1132, 1161
SsiI CCGC 2 cut(s) 376, 1615
SspMI CTAG 3 cut(s) 581, 1308, 1563
StyD4I CCNGG 4 cut(s) 119, 250, 700, 848
StyI CCWWGG 2 cut(s) 1562, 1664
TaaI ACNGT 5 cut(s) 34, 631, 673, 1493, 1659
TaiI ACGT 4 cut(s) 739, 922, 1000, 1471
TasI AATT 8 cut(s) 87, 137, 206, 712, 975, 1050, 1132, 1161
TatI WGTACW 1 cut(s) 263
TauI GCSGC 2 cut(s) 378, 1617
TfiI GAWTC 6 cut(s) 269, 791, 902, 1063, 1075, 1704
Tru1I TTAA 6 cut(s) 600, 633, 993, 1230, 1340, 1409
Tru9I TTAA 6 cut(s) 600, 633, 993, 1230, 1340, 1409
TscAI CASTG 2 cut(s) 963, 1291
TseFI GTSAC 1 cut(s) 1712
Tsp45I GTSAC 1 cut(s) 1712
TspDTI ATGAA 4 cut(s) 711, 1004, 1163, 1377
TspGWI ACGGA 2 cut(s) 413, 1061
TspRI CASTG 2 cut(s) 963, 1291
XagI CCTNNNNNAGG 1 cut(s) 825
XapI RAATTY 3 cut(s) 87, 137, 712
XmaJI CCTAGG 1 cut(s) 1562
XspI CTAG 3 cut(s) 581, 1308, 1563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.