Rroxscaffold_3G00272540

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005039
Physical Location & Seq
Reverse (-)
64633035 .. 64637090
4056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00272540.1

Sequence Viewer

Length: 888 bp
ATGCAAGTTACCTCTGAATCTGAGAAACCCAAACGTATCCTTAGGAAAGTTTCCAGCCAGCCAGCAGATCCAGTGCAGGAAAATCCACAAATTGAGCTTGAAAAGGTTAAACGTAACTTGAGGAAGGTTCATACTCCCATAGTAGAGAACTCTGTGCAGACGGAAATTGAAGCTCAGAATCTCACACAGAATCTGGAAAGGGCATCAAGTGTTTCGGGTCATAATGTTTTTGAAGGGATCACAAATAATTCTGTTGAGAAGATGAAGAATGAGCCAATTGTTGCTCTGTCCAATCTGCCTGATGCAGAAATAACTCCTGAACCATCAGCAGCAAAAGAGGTCTTTAACTTATTATCCGATGATCAGGTTGCAATGGATTCGAAGCCTTTGACAGAGGACACTGGTAAAGAAATAAATACACCTAGTGATGAAGCTGCAGTTGAGTCAAACATATTAACAGAGAGCAGTAAAAAAGATGAAAACATCCCTGTTCCAAATGTGGTTTTGAGCCAAAAGGAAGATTTAACAAGCAATGACAACCAGAAATCAAGCAGGAAAACTTCAACTCCAGCCAAACAAGAACGTTCAGAGAATGGGTTGCAAAACAGTCCAACATTGCCAAGCTATATGGCAGCAACTGAGTCTGCAAAGGCAAAGCTGAGGGCACAAGGATCTCCTAGGCTTGGACTGGAGGCTACTGAAAAGACTGAGAAAACTAACTCCACTCGCCGTCATTCTCTGCCATCATCAGCTAATGGAAAAATCACTTCACAGTCTCCAAGGACACAGAAACTTGTTCAATCAGGTGGAAAAGGAGGAATCAAAAGTGACAAATCTCTGTCAGCCTCAAGAGACGGGAAGGCAAGCCAGGCAGAGTGGAGAAGATGA

Protein Analysis

295

Amino Acids

32.16

Weight (kDa)

7.82

Isoelectric Point (pI)

61.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 179 - 276 2.7e-16 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 583
AclWI GGATC 3 cut(s) 62, 245, 679
AdeI CACNNNGTG 1 cut(s) 425
AfiI CCNNNNNNNGG 1 cut(s) 683
AgsI TTSAA 5 cut(s) 101, 170, 233, 564, 800
AjnI CCWGG 1 cut(s) 867
AluBI AGCT 6 cut(s) 97, 173, 434, 624, 658, 752
AluI AGCT 6 cut(s) 97, 173, 434, 624, 658, 752
Alw26I GTCTC 2 cut(s) 780, 846
AlwI GGATC 3 cut(s) 62, 245, 679
AlwNI CAGNNNCTG 2 cut(s) 193, 638
ApeKI GCWGC 3 cut(s) 329, 434, 632
AspA2I CCTAGG 1 cut(s) 677
AsuII TTCGAA 1 cut(s) 380
AvrII CCTAGG 1 cut(s) 677
AxyI CCTNAGG 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 667
BbvCI CCTCAGC 1 cut(s) 659
BbvI GCAGC 3 cut(s) 341, 421, 644
BccI CCATC 2 cut(s) 331, 751
BceAI ACGGC 1 cut(s) 714
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BciT130I CCWGG 1 cut(s) 869
BciVI GTATCC 1 cut(s) 47
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 2 cut(s) 780, 846
BfaI CTAG 2 cut(s) 423, 678
BfmI CTRYAG 1 cut(s) 435
BfuI GTATCC 1 cut(s) 47
BisI GCNGC 3 cut(s) 330, 435, 633
BlnI CCTAGG 1 cut(s) 677
BlsI GCNGC 3 cut(s) 331, 436, 634
Bme1390I CCNGG 1 cut(s) 869
BmrFI CCNGG 1 cut(s) 869
BmsI GCATC 2 cut(s) 212, 292
BpmI CTGGAG 2 cut(s) 552, 710
Bpu10I CCTNAGC 1 cut(s) 659
Bpu14I TTCGAA 1 cut(s) 380
BpuEI CTTGAG 2 cut(s) 139, 832
BsaJI CCNNGG 2 cut(s) 677, 779
BsaXI ACNNNNNCTCC 2 cut(s) 550, 580
Bsc4I CCNNNNNNNGG 1 cut(s) 683
Bse1I ACTGG 3 cut(s) 71, 406, 693
Bse21I CCTNAGG 1 cut(s) 41
Bse3DI GCAATG 3 cut(s) 378, 538, 614
BseBI CCWGG 1 cut(s) 869
BseDI CCNNGG 2 cut(s) 677, 779
BseGI GGATG 1 cut(s) 483
BseLI CCNNNNNNNGG 1 cut(s) 683
BseMI GCAATG 3 cut(s) 378, 538, 614
BseMII CTCAG 5 cut(s) 12, 188, 630, 650, 699
BseNI ACTGG 3 cut(s) 71, 406, 693
BseSI GKGCMC 1 cut(s) 667
BseXI GCAGC 3 cut(s) 341, 421, 644
BsgI GTGCAG 2 cut(s) 95, 176
BslI CCNNNNNNNGG 1 cut(s) 683
BsmAI GTCTC 2 cut(s) 780, 846
BsmBI CGTCTC 1 cut(s) 846
Bsp119I TTCGAA 1 cut(s) 380
Bsp1286I GDGCHC 1 cut(s) 667
Bsp143I GATC 4 cut(s) 67, 237, 361, 671
BspCNI CTCAG 5 cut(s) 13, 187, 631, 651, 700
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 3 cut(s) 62, 245, 679
BspT104I TTCGAA 1 cut(s) 380
BsrDI GCAATG 3 cut(s) 378, 538, 614
BsrI ACTGG 3 cut(s) 71, 406, 693
BssECI CCNNGG 2 cut(s) 677, 779
BssMI GATC 4 cut(s) 67, 237, 361, 671
BssT1I CCWWGG 2 cut(s) 677, 779
Bst2UI CCWGG 1 cut(s) 869
Bst4CI ACNGT 2 cut(s) 608, 774
BstBI TTCGAA 1 cut(s) 380
BstC8I GCNNGC 3 cut(s) 59, 63, 865
BstDEI CTNAG 6 cut(s) 21, 41, 174, 639, 659, 708
BstF5I GGATG 1 cut(s) 483
BstKTI GATC 4 cut(s) 70, 240, 364, 674
BstMAI GTCTC 2 cut(s) 780, 846
BstMBI GATC 4 cut(s) 67, 237, 361, 671
BstMWI GCNNNNNNNGC 1 cut(s) 869
BstNI CCWGG 1 cut(s) 869
BstSCI CCNGG 1 cut(s) 867
BstSFI CTRYAG 1 cut(s) 435
BstSLI GKGCMC 1 cut(s) 667
BstV1I GCAGC 3 cut(s) 341, 421, 644
BstX2I RGATCY 2 cut(s) 67, 671
BstYI RGATCY 2 cut(s) 67, 671
Bsu36I CCTNAGG 1 cut(s) 41
BsuI GTATCC 1 cut(s) 47
BtsCI GGATG 1 cut(s) 483
BtsIMutI CAGTG 2 cut(s) 78, 399
Cac8I GCNNGC 3 cut(s) 59, 63, 865
CaiI CAGNNNCTG 2 cut(s) 193, 638
DdeI CTNAG 6 cut(s) 21, 41, 174, 639, 659, 708
DpnI GATC 4 cut(s) 69, 239, 363, 673
DpnII GATC 4 cut(s) 67, 237, 361, 671
DraIII CACNNNGTG 1 cut(s) 425
Eco130I CCWWGG 2 cut(s) 677, 779
Eco81I CCTNAGG 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 867
EcoT14I CCWWGG 2 cut(s) 677, 779
ErhI CCWWGG 2 cut(s) 677, 779
Esp3I CGTCTC 1 cut(s) 846
FaiI YATR 6 cut(s) 132, 140, 222, 452, 627, 629
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 3 cut(s) 330, 435, 633
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 3 cut(s) 330, 435, 633
FspBI CTAG 2 cut(s) 423, 678
GluI GCNGC 3 cut(s) 330, 435, 633
GsuI CTGGAG 2 cut(s) 552, 710
HinfI GANTC 7 cut(s) 17, 178, 190, 377, 443, 641, 819
Hpy188I TCNGA 5 cut(s) 16, 22, 177, 358, 589
Hpy188III TCNNGA 3 cut(s) 194, 317, 849
HpyAV CCTTC 3 cut(s) 118, 227, 853
HpyCH4III ACNGT 2 cut(s) 608, 774
HpyCH4IV ACGT 3 cut(s) 34, 112, 583
HpyCH4V TGCA 8 cut(s) 4, 76, 157, 305, 371, 437, 601, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 869
HpyF3I CTNAG 6 cut(s) 21, 41, 174, 639, 659, 708
HpySE526I ACGT 3 cut(s) 34, 112, 583
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 4 cut(s) 67, 237, 361, 671
Lsp1109I GCAGC 3 cut(s) 341, 421, 644
LweI GCATC 2 cut(s) 212, 292
MaeI CTAG 2 cut(s) 423, 678
MaeII ACGT 3 cut(s) 34, 112, 583
MaeIII GTNAC 3 cut(s) 7, 113, 827
MalI GATC 4 cut(s) 69, 239, 363, 673
MboI GATC 4 cut(s) 67, 237, 361, 671
MboII GAAGA 3 cut(s) 271, 277, 530
MfeI CAATTG 1 cut(s) 276
MflI RGATCY 2 cut(s) 67, 671
MhlI GDGCHC 1 cut(s) 667
MluCI AATT 4 cut(s) 90, 165, 247, 276
MlyI GAGTC 2 cut(s) 452, 650
MmeI TCCRAC 1 cut(s) 635
MnlI CCTC 8 cut(s) 22, 114, 331, 388, 654, 685, 809, 856
MseI TTAA 4 cut(s) 108, 345, 455, 524
MspR9I CCNGG 1 cut(s) 869
MunI CAATTG 1 cut(s) 276
MvaI CCWGG 1 cut(s) 869
MwoI GCNNNNNNNGC 1 cut(s) 869
NdeII GATC 4 cut(s) 67, 237, 361, 671
NmuCI GTSAC 1 cut(s) 827
NspV TTCGAA 1 cut(s) 380
PfeI GAWTC 5 cut(s) 17, 178, 190, 377, 819
PkrI GCNGC 3 cut(s) 331, 436, 634
PleI GAGTC 2 cut(s) 451, 649
PpsI GAGTC 2 cut(s) 451, 649
Psp1406I AACGTT 1 cut(s) 583
Psp6I CCWGG 1 cut(s) 867
PspGI CCWGG 1 cut(s) 867
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 2 cut(s) 193, 638
PsuI RGATCY 2 cut(s) 67, 671
SaqAI TTAA 4 cut(s) 108, 345, 455, 524
SatI GCNGC 3 cut(s) 330, 435, 633
Sau3AI GATC 4 cut(s) 67, 237, 361, 671
SchI GAGTC 2 cut(s) 452, 650
ScrFI CCNGG 1 cut(s) 869
SduI GDGCHC 1 cut(s) 667
SfaNI GCATC 2 cut(s) 212, 292
SfcI CTRYAG 1 cut(s) 435
SfuI TTCGAA 1 cut(s) 380
SmlI CTYRAG 2 cut(s) 118, 847
SmoI CTYRAG 2 cut(s) 118, 847
Sse9I AATT 4 cut(s) 90, 165, 247, 276
SspMI CTAG 2 cut(s) 423, 678
StyD4I CCNGG 1 cut(s) 867
StyI CCWWGG 2 cut(s) 677, 779
TaaI ACNGT 2 cut(s) 608, 774
TaiI ACGT 3 cut(s) 37, 115, 586
TaqI TCGA 1 cut(s) 380
TasI AATT 4 cut(s) 90, 165, 247, 276
TfiI GAWTC 5 cut(s) 17, 178, 190, 377, 819
Tru1I TTAA 4 cut(s) 108, 345, 455, 524
Tru9I TTAA 4 cut(s) 108, 345, 455, 524
TscAI CASTG 2 cut(s) 78, 406
TseFI GTSAC 1 cut(s) 827
TseI GCWGC 3 cut(s) 329, 434, 632
Tsp45I GTSAC 1 cut(s) 827
TspDTI ATGAA 4 cut(s) 119, 278, 444, 492
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 2 cut(s) 78, 406
XmaJI CCTAGG 1 cut(s) 677
XspI CTAG 2 cut(s) 423, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.