Rh7DG037500

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0005039
Physical Location & Seq
Forward (+)
2767162 .. 2771625
4464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG037500.1

Sequence Viewer

Length: 1827 bp
ATGGAACTCTGCAGAAAACGGGCTTCAGGATTGTGTGTTATTAGCCTTCCTGCAATGGGCAAATCACCGGCTAAATGGATCAAGACTGTTCTGTTTGGGAAGAAGCCATCCAAACCTAATATATCAAAAGGAAGAGAGAGAATTTCAAATGAGAAAGAGGTGGTGGTTGCTGCCAGGGCAGCTGCAACTGAATTTGTTGCAGATCCACCTGCAGCTTTTCATGAAAGCACAAACACCACAGCTTATAACAAAGGGTTAGAATTGGAAAACGAGGAAGCTCCAAGCGTATTAGGTAATGGAGAGCCAGGAAGCCAAAGTACAGATTCACAAGGCTCTACACCACAAAATGCAGCGTATGATCCTGACAAAATCAGACAAGAGCAAGCTGCAACAAAGGCACAGGCTGCCTTTAGGGGTTATCTGGCTCGCCGCGCCTTTTGGGCCCTCAAAGGCATAATAAGGCTACAGGCACTTATCCGTGGGCACTTGGTGAGGAGACAAGCTGTTGCTACACTTTGCAGTATGTTGGGCATTGTCAAGCTACAGGCACTCAGTCGTGGACGACAAGTCAGGCAGTCTATTATTGGGCTTGAAGTGCAAAAAAGATGCCATGTTACGCCTCTGGAGGGCAAGCTAGTGGATCCTGTTGGAGTTAATATCTCTACTCAAATGGCAAAGCTGACGGTTAATGTTTTTATTAGTAAGCTTCTTGCTTTCTCACCTACTGTGATGCCTCTGCGTTTACATTATGAACCTGGAGATCCAAATTCAGTCCCAAACTGGTTAGAACGTTGGTCAGCAACACATTTTTGGAAACCACTTCCCCAACCAAGAAAAGTTCAAGATTCAAAATCTCAGAGAACACATCGGGATCCTCAAACAGGCAGGGTAAAGCGAAGCACCCGGAGGCTTCCGGCTGCAAATGTTGAAAGTGTCTCGGTGCAAGTTACCTCTGAATCTGAGAAACCCAAACGTATCCTTAGGAAAGTTTCCAGCCAGCCAGCAGATCCAGTGCAGGAAAATCCACAAATTGAGCTTGAAAAGGTTAAACGTAACTTGAGGAAGGTTCATACTCCCATAGTAGAGAACTCTGTGCAGACGGAAATTGAAGCTCAGAATCTCACACAGAATCTGGAAAAGGCATCAAGTGTTTCGGGTCATAATGTTTTTGAAGGGATCACAAATAATTCTGTTGAGAAGATGAAGAATGAGCCAATTGTTGCTCTGTCCAATCTGCCTGATGCAGAAATAACTCCTGAACCATCAGCAGCAAAAGAGGTCTTTAACTTATTATCCGATGATCAAGTTGCAATGGATTTGAAGCCTTTGACAGAGGCCACTGGTAAAGAAATAAATACACTTAGTGATGAAGCTGCAGTTGAGTCAAACATATTAACAGAGAGCAGTAAAAAAGATGAAAACATCCCTGTTCCAAGTGTGGTTTTGAGCCAAAAGGAAGATTTAACAAGCAATGACAACCAGAAATCAAGCAGGAAAACTTCAACTCCAGCCAAACAAGAACGTTCAGAGAATGGGTTGCAAAACAGTCCAACATTGCCAAGCTATATGGCAGCAACTGAGTCTGCAAAGGCAAAGCTGAGGGCACAAGGATCTCCTAGGCTTGGACTGGAGGCTACTGAAAAGACTGAGAAAACTAACTCCACTCGCCGCCATTCTCTGCCATCATCAGCTAATGGCAAAATCACTTCACAGTCTCCAAGGACACAGAAACTTGTTCAATCAGGTGGAAAAGGAGGAATCAAAGGTGACAAATCTCTGTCAGCCTCAAGAGACGGGAAGGCAAGCCAAGCAGAGTGGAGAAGATGA

Protein Analysis

608

Amino Acids

66.53

Weight (kDa)

9.77

Isoelectric Point (pI)

54.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 492 - 584 1.3e-15 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 246
AarI CACCTGC 1 cut(s) 217
Acc36I ACCTGC 1 cut(s) 217
AccII CGCG 1 cut(s) 432
AciI CCGC 2 cut(s) 430, 1669
AclI AACGTT 2 cut(s) 790, 1522
AcsI RAATTY 3 cut(s) 141, 191, 766
AcuI CTGAAG 1 cut(s) 9
AdeI CACNNNGTG 2 cut(s) 490, 1364
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 4 cut(s) 56, 626, 881, 1622
AhdI GACNNNNNGTC 1 cut(s) 566
AjnI CCWGG 3 cut(s) 173, 304, 754
Alw26I GTCTC 4 cut(s) 490, 940, 1719, 1785
AlwNI CAGNNNCTG 2 cut(s) 1132, 1577
AoxI GGCC 2 cut(s) 441, 1335
ApaI GGGCCC 1 cut(s) 445
ApoI RAATTY 3 cut(s) 141, 191, 766
AspA2I CCTAGG 1 cut(s) 1616
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 2 cut(s) 441, 442
AsuC2I CCSGG 1 cut(s) 904
AsuHPI GGTGA 4 cut(s) 57, 502, 711, 1778
AvrII CCTAGG 1 cut(s) 1616
AxyI CCTNAGG 1 cut(s) 980
BaeGI GKGCMC 3 cut(s) 445, 486, 1606
BamHI GGATCC 2 cut(s) 640, 871
BanII GRGCYC 1 cut(s) 445
BarI GAAGNNNNNNTAC 2 cut(s) 301, 333
BbvCI CCTCAGC 1 cut(s) 1598
BccI CCATC 3 cut(s) 115, 1270, 1690
BciT130I CCWGG 3 cut(s) 175, 306, 756
BciVI GTATCC 1 cut(s) 986
BclI TGATCA 1 cut(s) 1300
BcnI CCSGG 1 cut(s) 904
BcoDI GTCTC 4 cut(s) 490, 940, 1719, 1785
BfaI CTAG 2 cut(s) 635, 1617
BfmI CTRYAG 5 cut(s) 10, 210, 464, 542, 1374
BfuAI ACCTGC 1 cut(s) 217
BfuI GTATCC 1 cut(s) 986
BglI GCCNNNNNGGC 1 cut(s) 440
BlnI CCTAGG 1 cut(s) 1616
Bme1390I CCNGG 4 cut(s) 175, 306, 756, 904
BmeRI GACNNNNNGTC 1 cut(s) 566
BmgT120I GGNCC 2 cut(s) 441, 442
BmiI GGNNCC 3 cut(s) 443, 642, 873
BmrFI CCNGG 4 cut(s) 175, 306, 756, 904
BmsI GCATC 4 cut(s) 596, 720, 1151, 1231
BpmI CTGGAG 4 cut(s) 644, 777, 1491, 1649
Bpu10I CCTNAGC 1 cut(s) 1598
BpuEI CTTGAG 2 cut(s) 1078, 1771
BpuMI CCSGG 1 cut(s) 904
BsaJI CCNNGG 4 cut(s) 174, 478, 1616, 1718
BsaXI ACNNNNNCTCC 2 cut(s) 1489, 1519
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 626, 881, 1622
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 4 cut(s) 785, 1010, 1345, 1632
Bse21I CCTNAGG 1 cut(s) 980
Bse3DI GCAATG 4 cut(s) 60, 1317, 1477, 1553
BseBI CCWGG 3 cut(s) 175, 306, 756
BseDI CCNNGG 4 cut(s) 174, 478, 1616, 1718
BseGI GGATG 2 cut(s) 107, 1422
BseLI CCNNNNNNNGG 4 cut(s) 56, 626, 881, 1622
BseMI GCAATG 4 cut(s) 60, 1317, 1477, 1553
BseMII CTCAG 7 cut(s) 565, 869, 951, 1127, 1569, 1589, 1638
BseNI ACTGG 4 cut(s) 785, 1010, 1345, 1632
BseRI GAGGAG 1 cut(s) 508
BseSI GKGCMC 3 cut(s) 445, 486, 1606
BsgI GTGCAG 2 cut(s) 1034, 1115
Bsh1236I CGCG 1 cut(s) 432
BshFI GGCC 2 cut(s) 443, 1337
BsiSI CCGG 3 cut(s) 68, 904, 914
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 4 cut(s) 56, 626, 881, 1622
BsmAI GTCTC 4 cut(s) 490, 940, 1719, 1785
BsmBI CGTCTC 1 cut(s) 1785
BsmFI GGGAC 1 cut(s) 758
BsnI GGCC 2 cut(s) 443, 1337
Bsp120I GGGCCC 1 cut(s) 441
Bsp1286I GDGCHC 3 cut(s) 445, 486, 1606
BspACI CCGC 2 cut(s) 430, 1669
BspANI GGCC 2 cut(s) 443, 1337
BspCNI CTCAG 7 cut(s) 564, 868, 952, 1126, 1570, 1590, 1639
BspFNI CGCG 1 cut(s) 432
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 3 cut(s) 443, 642, 873
BspMAI CTGCAG 3 cut(s) 14, 214, 1378
BspMI ACCTGC 1 cut(s) 217
BsrDI GCAATG 4 cut(s) 60, 1317, 1477, 1553
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 4 cut(s) 785, 1010, 1345, 1632
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 4 cut(s) 174, 478, 1616, 1718
BssT1I CCWWGG 2 cut(s) 1616, 1718
Bst2UI CCWGG 3 cut(s) 175, 306, 756
Bst4CI ACNGT 5 cut(s) 88, 685, 727, 1547, 1713
Bst6I CTCTTC 1 cut(s) 127
BstAPI GCANNNNNTGC 1 cut(s) 404
BstC8I GCNNGC 6 cut(s) 384, 427, 632, 998, 1002, 1804
BstDEI CTNAG 9 cut(s) 551, 855, 960, 980, 1113, 1361, 1578, 1598, 1647
BstDSI CCRYGG 1 cut(s) 478
BstENI CCTNNNNNAGG 1 cut(s) 879
BstF5I GGATG 2 cut(s) 107, 1422
BstFNI CGCG 1 cut(s) 432
BstHHI GCGC 1 cut(s) 434
BstMAI GTCTC 4 cut(s) 490, 940, 1719, 1785
BstMWI GCNNNNNNNGC 8 cut(s) 176, 179, 395, 404, 431, 440, 595, 1808
BstNI CCWGG 3 cut(s) 175, 306, 756
BstSCI CCNGG 4 cut(s) 173, 304, 754, 902
BstSFI CTRYAG 5 cut(s) 10, 210, 464, 542, 1374
BstSLI GKGCMC 3 cut(s) 445, 486, 1606
BstUI CGCG 1 cut(s) 432
BstX2I RGATCY 6 cut(s) 202, 640, 760, 871, 1006, 1610
BstYI RGATCY 6 cut(s) 202, 640, 760, 871, 1006, 1610
Bsu36I CCTNAGG 1 cut(s) 980
BsuI GTATCC 1 cut(s) 986
BsuRI GGCC 2 cut(s) 443, 1337
BtgI CCRYGG 1 cut(s) 478
BtsCI GGATG 2 cut(s) 107, 1422
BtsIMutI CAGTG 2 cut(s) 1017, 1338
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 6 cut(s) 384, 427, 632, 998, 1002, 1804
CaiI CAGNNNCTG 2 cut(s) 1132, 1577
CciI TCATGA 1 cut(s) 220
CfoI GCGC 1 cut(s) 434
Cfr10I RCCGGY 1 cut(s) 67
Cfr13I GGNCC 2 cut(s) 441, 442
Csp6I GTAC 1 cut(s) 318
CspCI CAANNNNNGTGG 1 cut(s) 1796
CviAII CATG 2 cut(s) 221, 611
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 9 cut(s) 551, 855, 960, 980, 1113, 1361, 1578, 1598, 1647
DraIII CACNNNGTG 2 cut(s) 490, 1364
DriI GACNNNNNGTC 1 cut(s) 566
Eam1104I CTCTTC 1 cut(s) 127
Eam1105I GACNNNNNGTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 127
Eco130I CCWWGG 2 cut(s) 1616, 1718
Eco24I GRGCYC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 9
Eco81I CCTNAGG 1 cut(s) 980
EcoNI CCTNNNNNAGG 1 cut(s) 879
EcoO109I RGGNCCY 1 cut(s) 442
EcoRII CCWGG 3 cut(s) 173, 304, 754
EcoT14I CCWWGG 2 cut(s) 1616, 1718
EcoT38I GRGCYC 1 cut(s) 445
ErhI CCWWGG 2 cut(s) 1616, 1718
Esp3I CGTCTC 1 cut(s) 1785
FaeI CATG 2 cut(s) 224, 614
FaqI GGGAC 1 cut(s) 758
FatI CATG 2 cut(s) 220, 610
FbaI TGATCA 1 cut(s) 1300
FokI GGATG 2 cut(s) 94, 1409
FriOI GRGCYC 1 cut(s) 445
FspBI CTAG 2 cut(s) 635, 1617
GlaI GCGC 1 cut(s) 433
GsuI CTGGAG 4 cut(s) 644, 777, 1491, 1649
HaeIII GGCC 2 cut(s) 443, 1337
HapII CCGG 3 cut(s) 68, 904, 914
HhaI GCGC 1 cut(s) 434
Hin1II CATG 2 cut(s) 224, 614
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 8 cut(s) 323, 845, 956, 1117, 1129, 1382, 1580, 1758
HpaII CCGG 3 cut(s) 68, 904, 914
HphI GGTGA 4 cut(s) 57, 502, 711, 1778
Hpy166II GTNNAC 2 cut(s) 560, 743
Hpy188I TCNGA 7 cut(s) 374, 858, 955, 961, 1116, 1297, 1528
Hpy8I GTNNAC 2 cut(s) 560, 743
HpyAV CCTTC 4 cut(s) 56, 1057, 1166, 1792
HpyCH4III ACNGT 5 cut(s) 88, 685, 727, 1547, 1713
HpyCH4IV ACGT 4 cut(s) 790, 973, 1051, 1522
HpyF10VI GCNNNNNNNGC 8 cut(s) 176, 179, 395, 404, 431, 440, 595, 1808
HpyF3I CTNAG 9 cut(s) 551, 855, 960, 980, 1113, 1361, 1578, 1598, 1647
HpySE526I ACGT 4 cut(s) 790, 973, 1051, 1522
Hsp92II CATG 2 cut(s) 224, 614
HspAI GCGC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 1300
LmnI GCTCC 1 cut(s) 283
LweI GCATC 4 cut(s) 596, 720, 1151, 1231
MaeI CTAG 2 cut(s) 635, 1617
MaeII ACGT 4 cut(s) 790, 973, 1051, 1522
MaeIII GTNAC 4 cut(s) 613, 946, 1052, 1766
MboII GAAGA 5 cut(s) 112, 144, 1210, 1216, 1469
MfeI CAATTG 1 cut(s) 1215
MflI RGATCY 6 cut(s) 202, 640, 760, 871, 1006, 1610
MhlI GDGCHC 3 cut(s) 445, 486, 1606
MluCI AATT 8 cut(s) 141, 191, 260, 766, 1029, 1104, 1186, 1215
MlyI GAGTC 2 cut(s) 1391, 1589
MmeI TCCRAC 2 cut(s) 628, 1574
MseI TTAA 6 cut(s) 654, 687, 1047, 1284, 1394, 1463
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 3 cut(s) 68, 904, 914
MspR9I CCNGG 4 cut(s) 175, 306, 756, 904
MunI CAATTG 1 cut(s) 1215
MvaI CCWGG 3 cut(s) 175, 306, 756
MvnI CGCG 1 cut(s) 432
MwoI GCNNNNNNNGC 8 cut(s) 176, 179, 395, 404, 431, 440, 595, 1808
NciI CCSGG 1 cut(s) 904
NlaIII CATG 2 cut(s) 224, 614
NlaIV GGNNCC 3 cut(s) 443, 642, 873
NmuCI GTSAC 1 cut(s) 1766
PagI TCATGA 1 cut(s) 220
PaqCI CACCTGC 1 cut(s) 217
PfeI GAWTC 6 cut(s) 323, 845, 956, 1117, 1129, 1758
PleI GAGTC 2 cut(s) 1390, 1588
PpsI GAGTC 2 cut(s) 1390, 1588
PsiI TTATAA 1 cut(s) 246
Psp1406I AACGTT 2 cut(s) 790, 1522
Psp6I CCWGG 3 cut(s) 173, 304, 754
PspGI CCWGG 3 cut(s) 173, 304, 754
PspN4I GGNNCC 3 cut(s) 443, 642, 873
PspOMI GGGCCC 1 cut(s) 441
PspPI GGNCC 2 cut(s) 441, 442
PstI CTGCAG 3 cut(s) 14, 214, 1378
PstNI CAGNNNCTG 2 cut(s) 1132, 1577
PsuI RGATCY 6 cut(s) 202, 640, 760, 871, 1006, 1610
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
SaqAI TTAA 6 cut(s) 654, 687, 1047, 1284, 1394, 1463
Sau96I GGNCC 2 cut(s) 441, 442
SchI GAGTC 2 cut(s) 1391, 1589
ScrFI CCNGG 4 cut(s) 175, 306, 756, 904
SduI GDGCHC 3 cut(s) 445, 486, 1606
SfaNI GCATC 4 cut(s) 596, 720, 1151, 1231
SfcI CTRYAG 5 cut(s) 10, 210, 464, 542, 1374
SmlI CTYRAG 2 cut(s) 1057, 1786
SmoI CTYRAG 2 cut(s) 1057, 1786
Sse9I AATT 8 cut(s) 141, 191, 260, 766, 1029, 1104, 1186, 1215
SsiI CCGC 2 cut(s) 430, 1669
SspMI CTAG 2 cut(s) 635, 1617
StyD4I CCNGG 4 cut(s) 173, 304, 754, 902
StyI CCWWGG 2 cut(s) 1616, 1718
TaaI ACNGT 5 cut(s) 88, 685, 727, 1547, 1713
TaiI ACGT 4 cut(s) 793, 976, 1054, 1525
TasI AATT 8 cut(s) 141, 191, 260, 766, 1029, 1104, 1186, 1215
TatI WGTACW 1 cut(s) 317
TauI GCSGC 2 cut(s) 432, 1671
TfiI GAWTC 6 cut(s) 323, 845, 956, 1117, 1129, 1758
Tru1I TTAA 6 cut(s) 654, 687, 1047, 1284, 1394, 1463
Tru9I TTAA 6 cut(s) 654, 687, 1047, 1284, 1394, 1463
TscAI CASTG 2 cut(s) 1017, 1345
TseFI GTSAC 1 cut(s) 1766
Tsp45I GTSAC 1 cut(s) 1766
TspDTI ATGAA 7 cut(s) 209, 237, 765, 1058, 1217, 1383, 1431
TspGWI ACGGA 2 cut(s) 467, 1115
TspRI CASTG 2 cut(s) 1017, 1345
XagI CCTNNNNNAGG 1 cut(s) 879
XapI RAATTY 3 cut(s) 141, 191, 766
XmaJI CCTAGG 1 cut(s) 1616
XspI CTAG 2 cut(s) 635, 1617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.