Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006646

Physical Location & Seq

Genomic Coordinates Forward (+)

30287763 .. 30290299

Gene Structure 2537 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G80560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1218 bp

ATGGCGGCGGCTCTGCAGACGAATATCCGGACGGTCAAGGTTCCGGCTACGTTCAGAGCTGTAAGTAAACAGTCATTGGCACCCTTTAGAGTAAGATGTGCTGTTGCTTCCCCTGGTAAAAAACGATACACCATCACTCTCCTTCCCGGAGACGGCATCGGTCCGGAGGTTGTCTCCATTGCCAAAAATGTGCTTCAGCAAGCTGGATCTTTGGAAGGAGTGGAATTTAACTTCCGTGAGATGCCCATTGGAGGAGCTGCTTTGGATTTGGTCGGAGTGCCCTTGCCGGAGGAGACTATCTCAGCTGCAAAAGAATCAGATGCAGTGCTTCTTGGAGCCATTGGAGGGTACAAATGGGATAACAATGAAAAACATCTGAGGCCTGAGAAGGGGTTACTTCAGATTCGTGCAGCTCTCAAAGTCTTTGCAAATCTGAGACCTGCTACAGTCCTCCCACAGTTAGTGGATGCTTCCACCTTAAAGAGAGAGGTAGCAGAAGGTGTTGATCTAATGGTTGTAAGGGAGCTTACAGGAGGTATTTACTTTGGAGAGCCAAGGGGCATAAAGACCAATGAAAATGGCGAGGAAGTTGGGTTTAATACTGAGGTTTATGCTGCTCACGAGATTGATAGAATTGCTCGTGTTGCCTTCGAGACTGCTCGGAAACGGCGTGGCAAGCTGTGTTCTGTTGACAAAGCTAATGTTTTGGAAGCCTCAATCTTATGGAGGAAACGAGTAACAGCATTAGCCTCTGAATATCCTGATGTTGAGCTGTCACATATGTATGTTGACAATGCCGCGATGCAGCTTGTTCGTGACCCAAAACAGTTTGACACCATCGTCACAAACAACATTTTTGGTGATATATTATCCGATGAAGCTTCAATGATCACAGGAAGCATAGGAATGCTGCCATCTGCTAGTCTCAGTGATTCGGGACCTGGACTCTTTGAACCTATACATGGTTCTGCACCTGATATTGCTGGACAGGATAAGGCAAACCCATTGGCAACCATTCTAAGCGCTGCAATGCTTCTGAAATACGGACTTGGAGAAGAAAAGGCAGCCAAGAGAATTGAAGACGCAGTGTTGGTTGCTCTGAACAACGGATTCAGAACTGGAGACATATACTCCGCGGGAACAAAACTGGTGGGCTGCAAGGAGATGGGAGAGGAGGTTCTGAAGTCAGTGGACTCCCAAGTTCCAGCTTCTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0016143 GO:0016144 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0050896 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00290 ko00660 ko00966 ko01100 ko01110 ko01210 ko01230 map00290 map00660 map00966 map01100 map01110 map01210 map01230

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

405

Amino Acids

43.37

Weight (kDa)

5.8

Isoelectric Point (pI)

35.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	44 - 393	5.2e-145	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006646)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31180 AT1G31180 AT1G80560 AT5G14200 AT5G14200
fragaria_vesca	FvH4_2g04971
malus_domestica	MD17G1218700.v1.1
prunus_persica	Prupe.3G094300_v2.0.a1 Prupe.3G094300_v2.0.a1
pyrus_communis	pycom17g22270 pycom17g22330
rosa_chinensis	RchiOBHm_Chr2g0133781 RchiOBHm_Chr2g0133821
rosa_laevigata	RLG00000019364
rosa_multiflora	Rmu_sc0001782.1_g000049 Rmu_sc0001782.1_g000053 Rmu_sc0003682.1_g000003 Rmu_sc0004983.1_g000001
rosa_roxburghii	Rroxscaffold_2G00110790 Rroxscaffold_2G00110820
rosa_rugosa	Rorug02G0313900
rosa_samantha	Rh2AG367200 Rh2CG350300 Rh2CG350600 Rh2DG389700
rosa_wichuraiana	Rw2G029860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	839
Acc36I	ACCTGC	1 cut(s)	448
AccB1I	GGYRCC	1 cut(s)	79
AccII	CGCG	2 cut(s)	800, 1136
AccIII	TCCGGA	2 cut(s)	27, 163
AciI	CCGC	5 cut(s)	5, 8, 798, 1134, 1136
AclWI	GGATC	1 cut(s)	214
AcsI	RAATTY	1 cut(s)	224
AcuI	CTGAAG	3 cut(s)	179, 383, 1202
AfaI	GTAC	1 cut(s)	350
AfeI	AGCGCT	1 cut(s)	1024
AfiI	CCNNNNNNNGG	5 cut(s)	152, 251, 345, 389, 962
AgsI	TTSAA	3 cut(s)	885, 953, 1079
AjnI	CCWGG	2 cut(s)	112, 940
AloI	GAACNNNNNNTCC	2 cut(s)	1161, 1193
Alw26I	GTCTC	7 cut(s)	144, 178, 287, 430, 647, 929, 1116
AlwI	GGATC	1 cut(s)	214
AlwNI	CAGNNNCTG	1 cut(s)	1211
Aor13HI	TCCGGA	2 cut(s)	27, 163
Aor51HI	AGCGCT	1 cut(s)	1024
AoxI	GGCC	1 cut(s)	380
ApeKI	GCWGC	9 cut(s)	257, 305, 410, 614, 805, 910, 1025, 1064, 1155
ApoI	RAATTY	1 cut(s)	224
AspLEI	GCGC	1 cut(s)	1025
AspS9I	GGNCC	2 cut(s)	161, 938
AsuC2I	CCSGG	1 cut(s)	147
AsuHPI	GGTGA	1 cut(s)	872
AvaII	GGWCC	2 cut(s)	161, 938
BaeGI	GKGCMC	1 cut(s)	282
BanI	GGYRCC	1 cut(s)	79
BauI	CACGAG	2 cut(s)	620, 639
BbsI	GAAGAC	1 cut(s)	1086
BbvI	GCAGC	9 cut(s)	244, 292, 422, 601, 817, 897, 1012, 1076, 1142
BccI	CCATC	4 cut(s)	140, 845, 922, 1159
BceAI	ACGGC	2 cut(s)	169, 683
BcgI	CGANNNNNNTGC	2 cut(s)	794, 828
BciT130I	CCWGG	2 cut(s)	114, 942
BclI	TGATCA	1 cut(s)	888
BcnI	CCSGG	1 cut(s)	147
BcoDI	GTCTC	7 cut(s)	144, 178, 287, 430, 647, 929, 1116
BfaI	CTAG	1 cut(s)	921
BfmI	CTRYAG	2 cut(s)	14, 444
BfoI	RGCGCY	1 cut(s)	1026
BfuAI	ACCTGC	1 cut(s)	448
Bme1390I	CCNGG	3 cut(s)	114, 147, 942
Bme18I	GGWCC	2 cut(s)	161, 938
BmgT120I	GGNCC	2 cut(s)	161, 938
BmiI	GGNNCC	4 cut(s)	42, 81, 337, 939
BmrFI	CCNGG	3 cut(s)	114, 147, 942
BmsI	GCATC	5 cut(s)	165, 231, 310, 457, 792
BpiI	GAAGAC	1 cut(s)	1086
BplI	GAGNNNNNCTC	4 cut(s)	158, 190, 284, 316
BpmI	CTGGAG	1 cut(s)	1140
BpuMI	CCSGG	1 cut(s)	147
BsaI	GGTCTC	1 cut(s)	430
BsaJI	CCNNGG	3 cut(s)	112, 554, 1134
BsaWI	WCCGGW	2 cut(s)	27, 163
BsaXI	ACNNNNNCTCC	4 cut(s)	1115, 1145, 1161, 1191
Bsc4I	CCNNNNNNNGG	5 cut(s)	152, 251, 345, 389, 962
Bse1I	ACTGG	2 cut(s)	1123, 1152
Bse3DI	GCAATG	2 cut(s)	177, 1035
BseAI	TCCGGA	2 cut(s)	27, 163
BseBI	CCWGG	2 cut(s)	114, 942
BseDI	CCNNGG	3 cut(s)	112, 554, 1134
BseGI	GGATG	1 cut(s)	472
BseLI	CCNNNNNNNGG	5 cut(s)	152, 251, 345, 389, 962
BseMI	GCAATG	2 cut(s)	177, 1035
BseMII	CTCAG	6 cut(s)	315, 368, 375, 425, 594, 940
BseNI	ACTGG	2 cut(s)	1123, 1152
BseRI	GAGGAG	3 cut(s)	267, 305, 1187
BseSI	GKGCMC	1 cut(s)	282
BseXI	GCAGC	9 cut(s)	244, 292, 422, 601, 817, 897, 1012, 1076, 1142
BsgI	GTGCAG	2 cut(s)	429, 954
Bsh1236I	CGCG	2 cut(s)	800, 1136
BshFI	GGCC	1 cut(s)	382
BshNI	GGYRCC	1 cut(s)	79
BsiSI	CCGG	5 cut(s)	28, 44, 147, 164, 287
BslFI	GGGAC	1 cut(s)	951
BslI	CCNNNNNNNGG	5 cut(s)	152, 251, 345, 389, 962
BsmAI	GTCTC	7 cut(s)	144, 178, 287, 430, 647, 929, 1116
BsmBI	CGTCTC	1 cut(s)	144
BsmFI	GGGAC	1 cut(s)	951
BsmI	GAATGC	1 cut(s)	912
BsnI	GGCC	1 cut(s)	382
Bso31I	GGTCTC	1 cut(s)	430
Bsp1286I	GDGCHC	1 cut(s)	282
Bsp13I	TCCGGA	2 cut(s)	27, 163
Bsp143I	GATC	3 cut(s)	206, 505, 888
BspACI	CCGC	5 cut(s)	5, 8, 798, 1134, 1136
BspANI	GGCC	1 cut(s)	382
BspCNI	CTCAG	6 cut(s)	314, 369, 376, 426, 595, 939
BspEI	TCCGGA	2 cut(s)	27, 163
BspFNI	CGCG	2 cut(s)	800, 1136
BspLI	GGNNCC	4 cut(s)	42, 81, 337, 939
BspMAI	CTGCAG	1 cut(s)	18
BspMI	ACCTGC	1 cut(s)	448
BspPI	GGATC	1 cut(s)	214
BspT107I	GGYRCC	1 cut(s)	79
BspTNI	GGTCTC	1 cut(s)	430
BsrDI	GCAATG	2 cut(s)	177, 1035
BsrI	ACTGG	2 cut(s)	1123, 1152
BssECI	CCNNGG	3 cut(s)	112, 554, 1134
BssMI	GATC	3 cut(s)	206, 505, 888
BssSI	CACGAG	2 cut(s)	620, 639
BssT1I	CCWWGG	1 cut(s)	554
Bst2BI	CACGAG	2 cut(s)	620, 639
Bst2UI	CCWGG	2 cut(s)	114, 942
Bst4CI	ACNGT	5 cut(s)	34, 72, 448, 459, 828
BstC8I	GCNNGC	2 cut(s)	201, 677
BstDEI	CTNAG	7 cut(s)	301, 377, 384, 434, 603, 926, 1019
BstDSI	CCRYGG	1 cut(s)	1134
BstF5I	GGATG	1 cut(s)	472
BstFNI	CGCG	2 cut(s)	800, 1136
BstH2I	RGCGCY	1 cut(s)	1026
BstHHI	GCGC	1 cut(s)	1025
BstKTI	GATC	3 cut(s)	209, 508, 891
BstMAI	GTCTC	7 cut(s)	144, 178, 287, 430, 647, 929, 1116
BstMBI	GATC	3 cut(s)	206, 505, 888
BstMWI	GCNNNNNNNGC	2 cut(s)	644, 676
BstNI	CCWGG	2 cut(s)	114, 942
BstSCI	CCNGG	3 cut(s)	112, 145, 940
BstSFI	CTRYAG	2 cut(s)	14, 444
BstSLI	GKGCMC	1 cut(s)	282
BstUI	CGCG	2 cut(s)	800, 1136
BstV1I	GCAGC	9 cut(s)	244, 292, 422, 601, 817, 897, 1012, 1076, 1142
BstV2I	GAAGAC	1 cut(s)	1086
BstX2I	RGATCY	1 cut(s)	206
BstYI	RGATCY	1 cut(s)	206
BsuRI	GGCC	1 cut(s)	382
BtgI	CCRYGG	1 cut(s)	1134
BtgZI	GCGATG	1 cut(s)	815
BtsCI	GGATG	1 cut(s)	472
BtsI	GCAGTG	2 cut(s)	330, 1092
BtsIMutI	CAGTG	4 cut(s)	330, 934, 1092, 1194
BveI	ACCTGC	1 cut(s)	448
Cac8I	GCNNGC	2 cut(s)	201, 677
CaiI	CAGNNNCTG	1 cut(s)	1211
CfoI	GCGC	1 cut(s)	1025
Cfr13I	GGNCC	2 cut(s)	161, 938
Cfr42I	CCGCGG	1 cut(s)	1137
CpoI	CGGWCCG	1 cut(s)	161
CseI	GACGC	1 cut(s)	1091
Csp6I	GTAC	1 cut(s)	349
CspCI	CAANNNNNGTGG	2 cut(s)	1131, 1166
CspI	CGGWCCG	1 cut(s)	161
CviAII	CATG	1 cut(s)	962
CviQI	GTAC	1 cut(s)	349
DdeI	CTNAG	7 cut(s)	301, 377, 384, 434, 603, 926, 1019
DpnI	GATC	3 cut(s)	208, 507, 890
DpnII	GATC	3 cut(s)	206, 505, 888
DrdI	GACNNNNNNGTC	1 cut(s)	839
DseDI	GACNNNNNNGTC	1 cut(s)	839
Eco130I	CCWWGG	1 cut(s)	554
Eco147I	AGGCCT	1 cut(s)	382
Eco31I	GGTCTC	1 cut(s)	430
Eco47I	GGWCC	2 cut(s)	161, 938
Eco47III	AGCGCT	1 cut(s)	1024
Eco57I	CTGAAG	3 cut(s)	179, 383, 1202
EcoO109I	RGGNCCY	1 cut(s)	938
EcoRII	CCWGG	2 cut(s)	112, 940
EcoT14I	CCWWGG	1 cut(s)	554
ErhI	CCWWGG	1 cut(s)	554
Esp3I	CGTCTC	1 cut(s)	144
FaeI	CATG	1 cut(s)	965
FaqI	GGGAC	1 cut(s)	951
FatI	CATG	1 cut(s)	961
FauI	CCCGC	1 cut(s)	1129
FauNDI	CATATG	1 cut(s)	780
FbaI	TGATCA	1 cut(s)	888
FokI	GGATG	1 cut(s)	479
FspBI	CTAG	1 cut(s)	921
GlaI	GCGC	1 cut(s)	1024
GsuI	CTGGAG	1 cut(s)	1140
HaeII	RGCGCY	1 cut(s)	1026
HaeIII	GGCC	1 cut(s)	382
HapII	CCGG	5 cut(s)	28, 44, 147, 164, 287
HgaI	GACGC	1 cut(s)	1091
HhaI	GCGC	1 cut(s)	1025
Hin1II	CATG	1 cut(s)	965
Hin6I	GCGC	1 cut(s)	1023
HinP1I	GCGC	1 cut(s)	1023
HincII	GTYRAC	2 cut(s)	691, 790
HindII	GTYRAC	2 cut(s)	691, 790
HindIII	AAGCTT	1 cut(s)	879
HinfI	GANTC	6 cut(s)	314, 403, 932, 945, 1110, 1193
HpaII	CCGG	5 cut(s)	28, 44, 147, 164, 287
HphI	GGTGA	1 cut(s)	872
Hpy166II	GTNNAC	4 cut(s)	68, 691, 790, 1192
Hpy188III	TCNNGA	7 cut(s)	28, 164, 620, 652, 761, 815, 936
Hpy8I	GTNNAC	4 cut(s)	68, 691, 790, 1192
HpyAV	CCTTC	5 cut(s)	152, 209, 382, 491, 658
HpyCH4III	ACNGT	5 cut(s)	34, 72, 448, 459, 828
HpyCH4IV	ACGT	1 cut(s)	50
HpyCH4V	TGCA	9 cut(s)	16, 308, 323, 410, 428, 805, 971, 1028, 1158
HpyF10VI	GCNNNNNNNGC	2 cut(s)	644, 676
HpyF3I	CTNAG	7 cut(s)	301, 377, 384, 434, 603, 926, 1019
HpySE526I	ACGT	1 cut(s)	50
Hsp92II	CATG	1 cut(s)	965
HspAI	GCGC	1 cut(s)	1023
Kpn2I	TCCGGA	2 cut(s)	27, 163
Ksp22I	TGATCA	1 cut(s)	888
KspI	CCGCGG	1 cut(s)	1137
Kzo9I	GATC	3 cut(s)	206, 505, 888
LmnI	GCTCC	3 cut(s)	254, 335, 523
Lsp1109I	GCAGC	9 cut(s)	244, 292, 422, 601, 817, 897, 1012, 1076, 1142
LweI	GCATC	5 cut(s)	165, 231, 310, 457, 792
MaeI	CTAG	1 cut(s)	921
MaeII	ACGT	1 cut(s)	50
MaeIII	GTNAC	5 cut(s)	393, 736, 774, 815, 841
MalI	GATC	3 cut(s)	208, 507, 890
MboI	GATC	3 cut(s)	206, 505, 888
MboII	GAAGA	2 cut(s)	1067, 1091
MflI	RGATCY	1 cut(s)	206
MhlI	GDGCHC	1 cut(s)	282
MluCI	AATT	3 cut(s)	224, 633, 1074
MlyI	GAGTC	2 cut(s)	939, 1187
MmeI	TCCRAC	1 cut(s)	253
MroI	TCCGGA	2 cut(s)	27, 163
MseI	TTAA	4 cut(s)	228, 479, 597, 1216
MslI	CAYNNNNRTG	2 cut(s)	783, 905
MspA1I	CMGCKG	2 cut(s)	305, 1136
MspI	CCGG	5 cut(s)	28, 44, 147, 164, 287
MspR9I	CCNGG	3 cut(s)	114, 147, 942
Mva1269I	GAATGC	1 cut(s)	912
MvaI	CCWGG	2 cut(s)	114, 942
MvnI	CGCG	2 cut(s)	800, 1136
MwoI	GCNNNNNNNGC	2 cut(s)	644, 676
NciI	CCSGG	1 cut(s)	147
NdeI	CATATG	1 cut(s)	780
NdeII	GATC	3 cut(s)	206, 505, 888
NlaIII	CATG	1 cut(s)	965
NlaIV	GGNNCC	4 cut(s)	42, 81, 337, 939
NmuCI	GTSAC	3 cut(s)	774, 815, 841
PceI	AGGCCT	1 cut(s)	382
PcsI	WCGNNNNNNNCGW	1 cut(s)	667
PctI	GAATGC	1 cut(s)	912
PfeI	GAWTC	4 cut(s)	314, 403, 932, 1110
PfoI	TCCNGGA	1 cut(s)	145
PleI	GAGTC	2 cut(s)	939, 1187
PpsI	GAGTC	2 cut(s)	939, 1187
PpuMI	RGGWCCY	1 cut(s)	938
Psp5II	RGGWCCY	1 cut(s)	938
Psp6I	CCWGG	2 cut(s)	112, 940
PspGI	CCWGG	2 cut(s)	112, 940
PspN4I	GGNNCC	4 cut(s)	42, 81, 337, 939
PspPI	GGNCC	2 cut(s)	161, 938
PspPPI	RGGWCCY	1 cut(s)	938
PstI	CTGCAG	1 cut(s)	18
PstNI	CAGNNNCTG	1 cut(s)	1211
PsuI	RGATCY	1 cut(s)	206
PvuII	CAGCTG	1 cut(s)	305
RsaI	GTAC	1 cut(s)	350
RsaNI	GTAC	1 cut(s)	349
RseI	CAYNNNNRTG	2 cut(s)	783, 905
Rsr2I	CGGWCCG	1 cut(s)	161
RsrII	CGGWCCG	1 cut(s)	161
SacII	CCGCGG	1 cut(s)	1137
SaqAI	TTAA	4 cut(s)	228, 479, 597, 1216
Sau3AI	GATC	3 cut(s)	206, 505, 888
Sau96I	GGNCC	2 cut(s)	161, 938
SchI	GAGTC	2 cut(s)	939, 1187
ScrFI	CCNGG	3 cut(s)	114, 147, 942
SduI	GDGCHC	1 cut(s)	282
SfaNI	GCATC	5 cut(s)	165, 231, 310, 457, 792
SfcI	CTRYAG	2 cut(s)	14, 444
Sfr303I	CCGCGG	1 cut(s)	1137
SgrBI	CCGCGG	1 cut(s)	1137
SinI	GGWCC	2 cut(s)	161, 938
SmiMI	CAYNNNNRTG	2 cut(s)	783, 905
Sse9I	AATT	3 cut(s)	224, 633, 1074
SseBI	AGGCCT	1 cut(s)	382
SsiI	CCGC	5 cut(s)	5, 8, 798, 1134, 1136
SspMI	CTAG	1 cut(s)	921
StuI	AGGCCT	1 cut(s)	382
StyD4I	CCNGG	3 cut(s)	112, 145, 940
StyI	CCWWGG	1 cut(s)	554
TaaI	ACNGT	5 cut(s)	34, 72, 448, 459, 828
TaiI	ACGT	1 cut(s)	53
TaqI	TCGA	1 cut(s)	651
TaqII	GACCGA	1 cut(s)	149
TasI	AATT	3 cut(s)	224, 633, 1074
TauI	GCSGC	3 cut(s)	8, 11, 800
TfiI	GAWTC	4 cut(s)	314, 403, 932, 1110
Tru1I	TTAA	4 cut(s)	228, 479, 597, 1216
Tru9I	TTAA	4 cut(s)	228, 479, 597, 1216
TscAI	CASTG	4 cut(s)	330, 934, 1092, 1194
TseFI	GTSAC	3 cut(s)	774, 815, 841
TseI	GCWGC	9 cut(s)	257, 305, 410, 614, 805, 910, 1025, 1064, 1155
Tsp45I	GTSAC	3 cut(s)	774, 815, 841
TspDTI	ATGAA	3 cut(s)	381, 588, 891
TspGWI	ACGGA	3 cut(s)	224, 1059, 1122
TspRI	CASTG	4 cut(s)	330, 934, 1092, 1194
VpaK11BI	GGWCC	2 cut(s)	161, 938
XapI	RAATTY	1 cut(s)	224
XspI	CTAG	1 cut(s)	921

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.