RchiOBHm_Chr2g0133821

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006646
Physical Location & Seq
Reverse (-)
50712469 .. 50718211
5743 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50495

Sequence Viewer

Length: 1215 bp
ATGGCTTCTTGTCTGCAACTCAACGCTAAGCCCTTCACCCCTCTTGTCTCTCCGAACCCCCTCTCCAAACACACCTTCAAGCTTGGCAGGGTCCGGTGCTCCGCCGCCTCACCCTCCAAACGCTACACCATCACTCTGCTTCCCGGCGATGGCATTGGCCCCGAAGTCATTTCAGTCGCCAAAAACGTCCTCAACCTCGCTGCCACCATCGAAGGGATTGAGTTTAAATATCAAGAAATGCCTGTGGGTGGAAATGCCTTGGATTTGACTGGAGTGCCGTTACCAGAAGAGACCCTTTCGGCAGCTAAGCAATCCGATGCAGTTCTGCTGGGAGCAATTGGGGGGTACAAATGGGATAAAAACGAAAAGCATTTAAAACCAGAAACTGGGTTGCTTAAGCTTCGGGAAGGTCTTAAAGTGTTTGCAAATTTGAGGCCAGCAGTTGTTTTACCGCAGTTGGTCGATGCCTCTACTTTGAAGAGAGAGGTCGCCGAAGGGGTTGACCTTATGGTTATAAGAGAGCTCACTGGTGGTATATACTTTGGACAGCCAAGGGGTTTTGGCACTAATGAAAACGGTGAGGAGATTGGTTTCAATACCGAGGTGTATGCTGCCTATGAGATTGATCGTATTGCTCGAGTTGCATTTGAGACTGCTAGGAAGCGGCAGGGAAAGCTCTGTAGTGTTGACAAAGCAAATGTATTGGAGGCATCAATGTTTTGGAGAAAAAGAATCCTGGAAATAGCCTTGGATTATCCTGATGTTGAACTCTCACACATGTATGTTGATAATGCGGCAATGCAGCTTGTTCGTAATCCAAAACAGTTTGATACAATTGTAACAAACAACATATTTGGCGATATATTATCTGATGAGGCCTCAATGATTACTGGAAGTATTGGGATGCTTCCATCTGCTAGTCTTGGAGAATCAGGTCCTGGACTTTTTGAACCTATACATGGTTCTGCTCCTGATATTGCTGGACAGGATAAGGCAAACCCATTAGCAACGGTTCTCAGTGCTGCTATGCTTTTGAAGTACGGTCTTGGAGAAGAAAATGCTGCCAAGAGAATTGAGGCAGCAGTCCTAGATACTTTGAACCGGGGGTTTCGAACGGTTGATATCTATTCGTCTGGAACGAAGTTGGTAGGATGTAAAGAAATGGGTGAAGAGGTATTGAAGTCAGTTGACTCTCTGGTTCCTTCTCCGGTATGA

Protein Analysis

404

Amino Acids

43.53

Weight (kDa)

5.51

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 43 - 392 7.1e-140 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 515
AccB7I CCANNNNNTGG 1 cut(s) 386
AciI CCGC 5 cut(s) 102, 105, 452, 664, 794
AcsI RAATTY 1 cut(s) 427
AfaI GTAC 2 cut(s) 347, 1040
AfiI CCNNNNNNNGG 5 cut(s) 149, 213, 248, 386, 959
AflII CTTAAG 1 cut(s) 395
AflIII ACRYGT 1 cut(s) 777
AgsI TTSAA 8 cut(s) 79, 478, 595, 767, 950, 1036, 1099, 1180
AjnI CCWGG 2 cut(s) 735, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
AluBI AGCT 6 cut(s) 82, 305, 400, 523, 676, 805
AluI AGCT 6 cut(s) 82, 305, 400, 523, 676, 805
Alw21I GWGCWC 2 cut(s) 101, 525
Alw26I GTCTC 3 cut(s) 52, 284, 644
AlwNI CAGNNNCTG 2 cut(s) 386, 938
Ama87I CYCGRG 1 cut(s) 636
AoxI GGCC 3 cut(s) 157, 434, 876
ApeKI GCWGC 7 cut(s) 200, 302, 611, 802, 1022, 1061, 1079
ApoI RAATTY 1 cut(s) 427
AspS9I GGNCC 3 cut(s) 91, 158, 935
AsuC2I CCSGG 2 cut(s) 144, 1103
AsuHPI GGTGA 4 cut(s) 28, 102, 590, 1178
AsuII TTCGAA 1 cut(s) 1111
AvaI CYCGRG 1 cut(s) 636
AvaII GGWCC 2 cut(s) 91, 935
BaeI ACNNNNGTAYC 2 cut(s) 822, 855
BanII GRGCYC 1 cut(s) 525
Bbv12I GWGCWC 2 cut(s) 101, 525
BbvI GCAGC 7 cut(s) 187, 314, 598, 814, 1009, 1048, 1091
BccI CCATC 4 cut(s) 137, 143, 215, 919
BceAI ACGGC 1 cut(s) 262
BcgI CGANNNNNNTGC 4 cut(s) 590, 624, 791, 825
BciT130I CCWGG 2 cut(s) 737, 939
BcnI CCSGG 2 cut(s) 144, 1103
BcoDI GTCTC 3 cut(s) 52, 284, 644
BfaI CTAG 3 cut(s) 657, 918, 1088
BfmI CTRYAG 1 cut(s) 679
BfrI CTTAAG 1 cut(s) 395
BlpI GCTNAGC 2 cut(s) 27, 306
Bme1390I CCNGG 4 cut(s) 144, 737, 939, 1103
Bme18I GGWCC 2 cut(s) 91, 935
BmeT110I CYCGRG 1 cut(s) 636
BmgT120I GGNCC 3 cut(s) 91, 158, 935
BmiI GGNNCC 3 cut(s) 92, 160, 1200
BmrFI CCNGG 4 cut(s) 144, 737, 939, 1103
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 4 cut(s) 307, 454, 719, 894
BmuI ACTGGG 1 cut(s) 396
BpmI CTGGAG 1 cut(s) 291
Bpu1102I GCTNAGC 2 cut(s) 27, 306
Bpu14I TTCGAA 1 cut(s) 1111
BpuMI CCSGG 2 cut(s) 144, 1103
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 5 cut(s) 258, 551, 600, 747, 1102
BsaWI WCCGGW 2 cut(s) 93, 1207
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 5 cut(s) 149, 213, 248, 386, 959
Bse1I ACTGG 4 cut(s) 274, 391, 532, 895
Bse3DI GCAATG 1 cut(s) 804
BseBI CCWGG 2 cut(s) 737, 939
BseDI CCNNGG 5 cut(s) 258, 551, 600, 747, 1102
BseGI GGATG 2 cut(s) 909, 1157
BseLI CCNNNNNNNGG 5 cut(s) 149, 213, 248, 386, 959
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 1 cut(s) 1030
BseNI ACTGG 4 cut(s) 274, 391, 532, 895
BseRI GAGGAG 1 cut(s) 596
BseXI GCAGC 7 cut(s) 187, 314, 598, 814, 1009, 1048, 1091
BseYI CCCAGC 1 cut(s) 328
BshFI GGCC 3 cut(s) 159, 436, 878
BsiHKAI GWGCWC 2 cut(s) 101, 525
BsiHKCI CYCGRG 1 cut(s) 636
BsiSI CCGG 4 cut(s) 94, 144, 1102, 1208
BslI CCNNNNNNNGG 5 cut(s) 149, 213, 248, 386, 959
BsmAI GTCTC 3 cut(s) 52, 284, 644
BsnI GGCC 3 cut(s) 159, 436, 878
Bso31I GGTCTC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 636
Bsp119I TTCGAA 1 cut(s) 1111
Bsp1286I GDGCHC 2 cut(s) 101, 525
Bsp143I GATC 1 cut(s) 625
Bsp1720I GCTNAGC 2 cut(s) 27, 306
BspACI CCGC 5 cut(s) 102, 105, 452, 664, 794
BspANI GGCC 3 cut(s) 159, 436, 878
BspCNI CTCAG 1 cut(s) 1029
BspLI GGNNCC 3 cut(s) 92, 160, 1200
BspT104I TTCGAA 1 cut(s) 1111
BspTI CTTAAG 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 804
BsrI ACTGG 4 cut(s) 274, 391, 532, 895
BssECI CCNNGG 5 cut(s) 258, 551, 600, 747, 1102
BssMI GATC 1 cut(s) 625
BssT1I CCWWGG 3 cut(s) 258, 551, 747
Bst2UI CCWGG 2 cut(s) 737, 939
Bst4CI ACNGT 5 cut(s) 578, 825, 1012, 1043, 1117
Bst6I CTCTTC 3 cut(s) 282, 473, 1164
BstAFI CTTAAG 1 cut(s) 395
BstBI TTCGAA 1 cut(s) 1111
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 3 cut(s) 27, 306, 1016
BstF5I GGATG 2 cut(s) 909, 1157
BstKTI GATC 1 cut(s) 628
BstMAI GTCTC 3 cut(s) 52, 284, 644
BstMBI GATC 1 cut(s) 625
BstMWI GCNNNNNNNGC 2 cut(s) 641, 673
BstNI CCWGG 2 cut(s) 737, 939
BstNSI RCATGY 1 cut(s) 781
BstSCI CCNGG 4 cut(s) 142, 735, 937, 1101
BstSFI CTRYAG 1 cut(s) 679
BstV1I GCAGC 7 cut(s) 187, 314, 598, 814, 1009, 1048, 1091
BsuRI GGCC 3 cut(s) 159, 436, 878
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 2 cut(s) 909, 1157
BtsIMutI CAGTG 2 cut(s) 525, 1024
Cac8I GCNNGC 1 cut(s) 438
CaiI CAGNNNCTG 2 cut(s) 386, 938
Cfr13I GGNCC 3 cut(s) 91, 158, 935
Csp6I GTAC 2 cut(s) 346, 1039
CviAII CATG 2 cut(s) 778, 959
CviQI GTAC 2 cut(s) 346, 1039
DdeI CTNAG 3 cut(s) 27, 306, 1016
DpnI GATC 1 cut(s) 627
DpnII GATC 1 cut(s) 625
DraI TTTAAA 2 cut(s) 226, 375
Eam1104I CTCTTC 3 cut(s) 282, 473, 1164
EarI CTCTTC 3 cut(s) 282, 473, 1164
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 3 cut(s) 258, 551, 747
Eco147I AGGCCT 1 cut(s) 878
Eco24I GRGCYC 1 cut(s) 525
Eco31I GGTCTC 1 cut(s) 284
Eco32I GATATC 1 cut(s) 1123
Eco47I GGWCC 2 cut(s) 91, 935
Eco53kI GAGCTC 1 cut(s) 523
Eco88I CYCGRG 1 cut(s) 636
EcoICRI GAGCTC 1 cut(s) 523
EcoO109I RGGNCCY 1 cut(s) 935
EcoRII CCWGG 2 cut(s) 735, 937
EcoRV GATATC 1 cut(s) 1123
EcoT14I CCWWGG 3 cut(s) 258, 551, 747
EcoT38I GRGCYC 1 cut(s) 525
ErhI CCWWGG 3 cut(s) 258, 551, 747
FaeI CATG 2 cut(s) 781, 962
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FatI CATG 2 cut(s) 777, 958
FokI GGATG 2 cut(s) 916, 1164
FriOI GRGCYC 1 cut(s) 525
FspBI CTAG 3 cut(s) 657, 918, 1088
GsaI CCCAGC 1 cut(s) 332
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 3 cut(s) 159, 436, 878
HapII CCGG 4 cut(s) 94, 144, 1102, 1208
Hin1II CATG 2 cut(s) 781, 962
HincII GTYRAC 3 cut(s) 502, 688, 1189
HindII GTYRAC 3 cut(s) 502, 688, 1189
HindIII AAGCTT 2 cut(s) 80, 398
HinfI GANTC 3 cut(s) 732, 929, 1190
HpaII CCGG 4 cut(s) 94, 144, 1102, 1208
HphI GGTGA 4 cut(s) 28, 102, 590, 1178
Hpy166II GTNNAC 3 cut(s) 502, 688, 1189
Hpy188I TCNGA 3 cut(s) 54, 316, 871
Hpy188III TCNNGA 5 cut(s) 233, 404, 758, 971, 1134
Hpy8I GTNNAC 3 cut(s) 502, 688, 1189
HpyAV CCTTC 6 cut(s) 43, 85, 206, 401, 488, 1212
HpyCH4III ACNGT 5 cut(s) 578, 825, 1012, 1043, 1117
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 5 cut(s) 16, 320, 425, 644, 802
HpyF10VI GCNNNNNNNGC 2 cut(s) 641, 673
HpyF3I CTNAG 3 cut(s) 27, 306, 1016
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 2 cut(s) 781, 962
Kzo9I GATC 1 cut(s) 625
LmnI GCTCC 3 cut(s) 104, 332, 973
Lsp1109I GCAGC 7 cut(s) 187, 314, 598, 814, 1009, 1048, 1091
LweI GCATC 4 cut(s) 307, 454, 719, 894
MaeI CTAG 3 cut(s) 657, 918, 1088
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 279, 838
MalI GATC 1 cut(s) 627
MboI GATC 1 cut(s) 625
MboII GAAGA 4 cut(s) 299, 490, 1064, 1181
MfeI CAATTG 2 cut(s) 336, 834
MhlI GDGCHC 2 cut(s) 101, 525
MluCI AATT 4 cut(s) 336, 427, 834, 1071
MlyI GAGTC 1 cut(s) 1184
MseI TTAA 4 cut(s) 225, 374, 396, 414
MslI CAYNNNNRTG 1 cut(s) 780
MspCI CTTAAG 1 cut(s) 395
MspI CCGG 4 cut(s) 94, 144, 1102, 1208
MspR9I CCNGG 4 cut(s) 144, 737, 939, 1103
MunI CAATTG 2 cut(s) 336, 834
MvaI CCWGG 2 cut(s) 737, 939
MwoI GCNNNNNNNGC 2 cut(s) 641, 673
NciI CCSGG 2 cut(s) 144, 1103
NdeII GATC 1 cut(s) 625
NlaIII CATG 2 cut(s) 781, 962
NlaIV GGNNCC 3 cut(s) 92, 160, 1200
NspI RCATGY 1 cut(s) 781
NspV TTCGAA 1 cut(s) 1111
PaeR7I CTCGAG 1 cut(s) 636
PceI AGGCCT 1 cut(s) 878
PciI ACATGT 1 cut(s) 777
PcsI WCGNNNNNNNCGW 3 cut(s) 183, 634, 1136
PfeI GAWTC 2 cut(s) 732, 929
PflMI CCANNNNNTGG 1 cut(s) 386
PfoI TCCNGGA 2 cut(s) 735, 937
PleI GAGTC 1 cut(s) 1184
PpsI GAGTC 1 cut(s) 1184
PpuMI RGGWCCY 1 cut(s) 935
PscI ACATGT 1 cut(s) 777
PsiI TTATAA 1 cut(s) 515
Psp124BI GAGCTC 1 cut(s) 525
Psp5II RGGWCCY 1 cut(s) 935
Psp6I CCWGG 2 cut(s) 735, 937
PspFI CCCAGC 1 cut(s) 328
PspGI CCWGG 2 cut(s) 735, 937
PspN4I GGNNCC 3 cut(s) 92, 160, 1200
PspPI GGNCC 3 cut(s) 91, 158, 935
PspPPI RGGWCCY 1 cut(s) 935
PspXI VCTCGAGB 1 cut(s) 636
PstNI CAGNNNCTG 2 cut(s) 386, 938
RsaI GTAC 2 cut(s) 347, 1040
RsaNI GTAC 2 cut(s) 346, 1039
RseI CAYNNNNRTG 1 cut(s) 780
SacI GAGCTC 1 cut(s) 525
SaqAI TTAA 4 cut(s) 225, 374, 396, 414
Sau3AI GATC 1 cut(s) 625
Sau96I GGNCC 3 cut(s) 91, 158, 935
SchI GAGTC 1 cut(s) 1184
ScrFI CCNGG 4 cut(s) 144, 737, 939, 1103
SduI GDGCHC 2 cut(s) 101, 525
SfaNI GCATC 4 cut(s) 307, 454, 719, 894
SfcI CTRYAG 1 cut(s) 679
Sfr274I CTCGAG 1 cut(s) 636
SfuI TTCGAA 1 cut(s) 1111
SinI GGWCC 2 cut(s) 91, 935
SlaI CTCGAG 1 cut(s) 636
SmiMI CAYNNNNRTG 1 cut(s) 780
SmlI CTYRAG 2 cut(s) 395, 636
SmoI CTYRAG 2 cut(s) 395, 636
Sse9I AATT 4 cut(s) 336, 427, 834, 1071
SseBI AGGCCT 1 cut(s) 878
SsiI CCGC 5 cut(s) 102, 105, 452, 664, 794
SspMI CTAG 3 cut(s) 657, 918, 1088
SstI GAGCTC 1 cut(s) 525
StuI AGGCCT 1 cut(s) 878
StyD4I CCNGG 4 cut(s) 142, 735, 937, 1101
StyI CCWWGG 3 cut(s) 258, 551, 747
TaaI ACNGT 5 cut(s) 578, 825, 1012, 1043, 1117
TaiI ACGT 1 cut(s) 189
TaqI TCGA 4 cut(s) 210, 462, 637, 1111
TasI AATT 4 cut(s) 336, 427, 834, 1071
TauI GCSGC 3 cut(s) 107, 667, 797
TfiI GAWTC 2 cut(s) 732, 929
Tru1I TTAA 4 cut(s) 225, 374, 396, 414
Tru9I TTAA 4 cut(s) 225, 374, 396, 414
TscAI CASTG 2 cut(s) 532, 1024
TseI GCWGC 7 cut(s) 200, 302, 611, 802, 1022, 1061, 1079
TspDTI ATGAA 1 cut(s) 585
TspRI CASTG 2 cut(s) 532, 1024
Van91I CCANNNNNTGG 1 cut(s) 386
Vha464I CTTAAG 1 cut(s) 395
VpaK11BI GGWCC 2 cut(s) 91, 935
XapI RAATTY 1 cut(s) 427
XceI RCATGY 1 cut(s) 781
XhoI CTCGAG 1 cut(s) 636
XspI CTAG 3 cut(s) 657, 918, 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.