Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007588

Physical Location & Seq

Genomic Coordinates Forward (+)

5694381 .. 5706442

Gene Structure 12062 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G13680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5772 bp

ATGGCACAGAGTAGTACATCTCATGACTCAGGCCCTCAGGGGCTCATGAGACGGCCATCTCGTAGTGCTGCCACAACAGTGTCTATTGAAGTTTTTGATCATGAGGTGGTTCCGGCCTCCCTTGGTACCATTGCTCCTATTCTGCGTGTTGCTGCAGAGATTGAACATGAGCGCCCTCGTGTTGCCTATCTCTGTCGGTTTTATGCATTTGAGAAAGCTCACAGGTTGGATCCAAGCTCTGGTGGTCGAGGTGTGAGGCAGTTTAAGACACTTCTCTTTCAAAGATTAGAAAGGGACAATGCTTCCAGTCTTGCTTCTAGGGTGAAAAAAACTGATGGACGGGAAGTTGAGAGCTTCTATCAACAATATTATGAGCACTATGTCAGGGCACTTGATCAAGGAGATCAAGCAGACAGAGCTCAGCTTGGTAAAGCATACCAGACGGCTGGAGTGCTCTTTGAAGTGCTCATGGCAGTTAACAAGAGCGAGAAAGTTGAAGCAGTAGCTCCTGAGATTATTGCAGCTGCTAGAGATGTCCAAGAAAAGAACGAAATTTATGCACCCTACAACATTCTTCCTTTGGATTCTGCTGGGGCCTCGCAGTCTGTTATGCAGCTTGAGGAGGTCAAAGCCGCTGTAGCTGCTTTGGGGAACACTCGTGGATTGAACTGGCCATCTGGGTTTGAGCAGCACCGAAAGAAAACCGGAAATCTGGATCTTCTTGACTGGTTAAGAGCTATGTTCGGATTCCAGAGAGACAATGTCAGAAACCAGAGGGAACATCTGGTTTGTTTATTTGCTGATAATCACATAAGATTGACTCCCAAGCCAGAACCTCTGAACAAGCTCGATGACAGAGCCGTTGATACTGTCATGTCAAAGCTCTTTAAGAACTACAAGAATTGGTGCAAATTTTTAGGACGTAAACACAGTTTAAGGCTTCCTCAAGCTGCTCAAGATATACAACAGCGGAAGATACTATATATGGGCTTGTATCTTCTCATCTGGGGAGAAGCTGCAAATATTCGCTTCATGCCAGAATGCTTGTGCTATATTTTCCACAATATGGCTTATGAACTCCATGGTCTCTTGGCTGGAAATGTCAGCATCGTTACCGGAGAAAATATTAAGCCTTCTTACGGTGGAGATGATGAGGCATTTTTAAGGAAGGTCATTACACCAATCTACCGTGTAGTTCAAACGGAAGCAAACAAGAATGCAAATGGCAAGGCTGCTCACTCAGATTGGTCCAACTATGATGATCTGAACGAATATTTCTGGACTCCTGATTGCTTCTCTCTTGGCTGGCCAATGCGTGATGATGGGGATCTTTTCAAATCAACCCGTGATACGACACAGGGAAAGAAAGGATCGTTTAGAAAAGCAGGACGCACAGGCAAATCAAATTTCACCGAAACTCGGACATTTTGGCACATATATCATAGTTTTGACCGACTCTGGACATTCTATCTATTAGCCCTGCAGGCAATGATCATTCTAGCATTTGAACGAGTAGAGCTGAGAGAAATCTTACGCAAAGATGTTTTATATGCTCTCTCAAGTATCTTCATCACAGCAGCTTTTCTGCGCTTCCTTCAGAGTGTCCTGGACGTTATCTTAAACTTCCCAGGTTTCCACAGGTGGAAATTTACTGATGTTTTGAGAAATATTCTCAAGATCGTTGTCAGTCTCGCTTGGTGTGTTGTGTTACCCCTTTGCTATGCCCAGTCTGTCTCTTTTGCTCCTGGGAAGCTGAAACAATGGCTATCATTTCTTCCACAAGTTAAAGGCGTTCCTCCTCTTTATATCATGGCTGTTGCACTGTACTTGCTTCCAAATGTACTGGCAGCTATCATGTTCATTTTTCCAATGCTTCGACGCTGGATCGAGAATTCAGACTGGCATATATTTAGATTGCTTCTGTGGTGGTCACAGCCAAGAATTTATGTTGGAAGAGGGATGCATGAAAGTCAAATTGCACTCATAAAGTATACGATTTTCTGGCTGTTGCTTTTCTGCTGCAAATTTGCATTCAGCTACTTTCTACAGGTGAAACTTTTGGTGAAGCCAACTAACGCAATTATGAGCATTCGCCATGTAAAATACAAATGGCATGAGTTTTTCCCCAATGCTGAGCACAACTATGGAGCTGTGGTGTCACTCTGGTTACCAGTAATTCTGGTTTACTTTATGGACACTCAAATTTGGTATGCTATTTTCTCCACTATATGTGGTGGTGTCATTGGGGCCTTTGATCGTTTGGGAGAGATACGAACGCTTGGAATGCTAAGGTCAAGGTTCCAATCATTACCTGGTGCATTTAATACGTACCTGGTTCCTTCTGATAAAACAAGGAGAAGAGGGTTTTCCTTGTCAAAAAGATTTGCAGAGGTCACTGCAGCAAGAAGAACTGAAGCGGCAAAATTCTCCCAACTCTGGAATGAAATCATTTCCAGCTTCCGAGAGGAAGATCTAATCAGTGACAGGGAGATGGATCTGTTGCTTGTTCCTTATACTTCAGACCCTAGTTTGAAACTAATTCAGTGGCCACCATTTTTGCTTGCAAGCAAGATACCGATAGCATTGGATATGGCAGCTCAGTTTCGAACAAGAGATTCTGACCTCTGGAAGCGCATATGCGCAGACGAATATATGAAATGTGCTGTCATTGAATGCTACGAATCTTTCAAACATGTCTTGCACACCTTGGTAATTGGGGAGAATGAGAAAAGGATTATTGGTATTATCATCAAAGAGGTGGAGAGCAACATATCAAAGAATTCCTTCCTTTCGAATTTCAGAATGGCGCCTTTACCTGCTCTTTGTAGTAAATTTGTCGAGCTTGTTGGAATTTTGAAAAATGCGGACCCAGCGAAAAGAGACACAGTGGTGCTATTGTTGCAAGATATGTTAGAAGTAGTGACTCGAGATATGATGCAAAATGAAAACCGTGAATTGGTAGAACTTGGGCATACTAACAAGGAATCCGGAAGGCAACTCTTTGCTGGTACTGACGCAAAGCCTGCTATACTGTTTCCTCCAGTGGCAACAGCTCAATGGCATGAACAGATAAGTCGCCTTCATTTACTTTTGACGGTAAAAGAATCTGCAATGGATGTTCCAACAAACCTTGAAGCACAAAGAAGGATTGCCTTCTTCACAAATTCACTGTTTATGGATATGCCACGAGCTCCTCGTGTCAGAAATATGCTGTCCTTCAGTGTTCTGACTCCGTACTATAGTGAGGAAACCGTCTACTCTAAGAACGACCTGGAAATGGAGAATGAAGATGGTGTATCAGTTGTATACTATTTGCAGAAGATCTTTCCAGATGAGTGGACTAACTTCTTGGAGCGGCTTGATTGTAAAGATGAAACTTCTGTTTTGGAGAGTGAAGAAAATATATTGCAGCTTCGCCATTGGGTTTCTTTGAGGGGACAGACTTTGTTTAGAACAGTTAGAGGAATGATGTATTACAGGCGGGCATTAAAGCTTCAGGCTTTTCTTGACATGGCTAATGAAACAGAAATATTAGCAGGGTACAAAGCCATTTCAGAGCCAACTGAGGAGGACAAGAAAAGTCAGAGATCCTTGTACACTCAGTTAGAGGCAGTGGCAGATCTCAAATTTACTTATGTTGCTACCTGTCAGAATTATGGAAATCAGAAGCGAAGTGGAGACCGGAGAGCTACTGACATCTTGAACCTGATGGTTAACAATCCCTCTCTCCGTGTAGCATACATTGATGAGGTTGAGGAACGAGAAGGCGGAAAAGTGCAGAAAGTTTTCTACTCGGTGCTTATCAAGGCTGTTGATAATCTTGATCAGGAAATATATCGGATAAAACTACCTGGACCAGCAAAAATTGGAGAAGGAAAACCCGAAAATCAGAATCATGCTCTCATTTTTACTAGAGGAGAAGCTCTCCAGGCTATTGACATGAATCAGGACCATTACTTGGAAGAAGCACTTAAAATGAGGAATCTGCTGGAAGAGTTCAATGAAGACCATGGAGTACGTGCACCCACTATTCTTGGTTTCAGAGAGCATATTTTTACTGGCAGTGTTTCTTCTTTAGCCTGGTTTATGTCAAATCAGGAAACAAGTTTTGTGACCATTGGTCAGCGAGTCCTGGCAAGTCCTTTGAAGGTAAGGTTTCACTATGGACACCCTGATGTCTTCGATAGAATCTTCCACATCACTCGGGGTGGCATCAGCAAAGCTTCACGGGGCATAAATCTTAGCGAGGATATCTTTGCTGGTTTCAACTCAACTCTGAGGCGTGGTAATGTCACCCATCATGAGTACATCCAAGTAGGGAAAGGAAGAGATGTTGGTTTGAACCAGATATCACTCTTTGAAGCCAAGGTTGCTTGTGGAAACGGAGAGCAAACATTGAGTAGGGATCTCTACCGACTTGGGCATCGTTTTGACTTTTTCCGCATGATGTCTTGTTATTTCACAACTGTAGGCTTCTACATCAGCTCGATGATTGTTGTCCTCACAGTTTATGCATTCTTGTATGGAAGACTTTACTTGTCATTGAGTGGAGTGGAAGAGGCGATAGTAAAGTTTGCAGCAGCGAAAGGAGATAGTTCTCTTAAGGCAGCGATGGCGTCACAGTCCGTAGTGCAATTGGGTCTGCTGATGACACTACCAATGGTGATGGAGATTGGGCTAGAGAGAGGATTCAGAACGGCGTTGTCTGATCTAATCATAATGCAGCTTCAGTTAGCGCCTGTCTTCTTCACCTTCTCACTTGGAACAAAGGTTCATTACTATGGTCGGACCATCCTACATGGGGGATCAAAGTACAGAGCAACAGGGCGTGGGTTTGTGGTGAAGCACGAGAAGTTTGCAGAGAACTACAGAATGTATTCGAGAAGCCACTTTGTGAAAGGAATGGAGCTGATGGTGCTGCTGATATGTTACAGGATTTATGGGAAAGCAGCAGAGGACTCGGTGGGTTATGCGCTTGTCATGGGCTCAACATGGTTCCTGGTGGGTTCCTGGTTGTTCGCCCCATTCTTTTTCAATCCCTCGGGATTCGAGTGGCAGAAGATAGTTGATGACTGGGATGATTGGAACAAGTGGATAAGCAGCAGAGGAGGTATCGGAGTACCAGCAAACAAAAGCTGGGAATCATGGTGGGAAGAGGAACAAGAGCATCTTCTTCACTCTGGGTTTTTCGGAAAGTTCTGGGAGATATTTCTCTCACTGCGGTACTTCATTTACCAGTATGGTATTGTGTACCAATTGAACTTGACCAAGGAGAGCCGAATGGGAAAACAGCACAGCATCATTGTGTATGGACTGTCTTGGCTGGTGATCGTGGCAGTGATGATAGTCCTCAAGATAGTGTCGATGGGGAGAAAGAAGTTCAGTGCTGATTTCCAGCTTATGTTCAGGCTTTTGAAGCTGTTTCTGTTCATAGGATCAGTGGTGATCGTGGGGATGCTATTCCACTTCTTGAAGCTGACCGTGGGAGACATAATGCAGTCTCTGTTGGCGTTTTTACCCACGGGATGGGCTCTCCTTCAGATATCACAAGTGGCGAGGCCATTGATGAAGACAGTAGGAATGTGGGGATCGGTCAAGGCCTTGGCTAGAGGGTATGAGTACATCATGGGAGTTGTTATCTTCATGCCAGTTACTGTACTCGCGTGGTTCCCTTTCGTCTCTGAGTTCCAGACAAGGCTTCTCTTCAATCAAGCCTTTAGCCGTGGTCTCCAGATCCAACGTATTCTCGCCGGTGGTAAGAAGCAGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006075 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009555 GO:0009556 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010208 GO:0010769 GO:0010927 GO:0016043 GO:0016049 GO:0016051 GO:0019953 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034293 GO:0034637 GO:0034645 GO:0040007 GO:0040008 GO:0043062 GO:0043170 GO:0043900 GO:0043934 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048236 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048646 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051273 GO:0051274 GO:0051321 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0080092 GO:0085029 GO:1901576 GO:1903046 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1923

Amino Acids

220.66

Weight (kDa)

9.1

Isoelectric Point (pI)

41.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vta1	PF04652	44 - 175	3.6e-12	Vta1 like
FKS1_dom1	PF14288	325 - 436	9.4e-42	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	850 - 1033	8.6e-68	Callose synthase 1 domain
Glucan_synthase	PF02364	1040 - 1125	2.8e-26	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	1131 - 1732	6.6e-207	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0007588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13680
fragaria_vesca	FvH4_1g24300
malus_domestica	MD01G1004000.v1.1 MD01G1004100.v1.1 MD02G1211500.v1.1
prunus_persica	Prupe.4G271000_v2.0.a1
pyrus_communis	pycom01g02040 pycom15g30380
rosa_chinensis	RchiOBHm_Chr2g0120781
rosa_laevigata	RLG00000018581 RLG00000018588
rosa_roxburghii	Rroxscaffold_2G00122840 Rroxscaffold_2G00122950
rosa_rugosa	Rorug02G0231300
rosa_samantha	Rh2AG287900 Rh2AG288000 Rh2AG288100 Rh2BG299200 Rh2BG299300 Rh2CG277200 Rh2CG277300 Rh2DG313100 Rh2DG313200
rosa_wichuraiana	Rw2G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2641
Acc36I	ACCTGC	1 cut(s)	2824
Acc65I	GGTACC	1 cut(s)	125
AccB1I	GGYRCC	2 cut(s)	125, 2806
AccB7I	CCANNNNNTGG	4 cut(s)	239, 1066, 3958, 5526
AccBSI	CCGCTC	1 cut(s)	3355
AccI	GTMKAC	3 cut(s)	1991, 3255, 3306
AccII	CGCG	1 cut(s)	5663
AccIII	TCCGGA	1 cut(s)	2987
AciI	CCGC	9 cut(s)	633, 970, 2417, 2864, 3355, 3481, 3768, 4441, 5221
AcoI	YGGCCR	4 cut(s)	53, 671, 1307, 2546
AcuI	CTGAAG	7 cut(s)	1580, 2433, 2502, 3202, 3479, 4712, 5522
AcyI	GRCGYC	2 cut(s)	2807, 4616
AdeI	CACNNNGTG	1 cut(s)	4894
AflII	CTTAAG	1 cut(s)	4601
AflIII	ACRYGT	1 cut(s)	2692
AhdI	GACNNNNNGTC	1 cut(s)	4119
AleI	CACNNNNGTG	2 cut(s)	77, 2888
AloI	GAACNNNNNNTCC	2 cut(s)	4755, 4787
Alw21I	GWGCWC	7 cut(s)	378, 421, 456, 468, 2139, 3193, 4024
Alw44I	GTGCAC	1 cut(s)	4020
AlwNI	CAGNNNCTG	2 cut(s)	5503, 5654
Ama87I	CYCGRG	3 cut(s)	2925, 4203, 5041
Aor13HI	TCCGGA	1 cut(s)	2987
ApaLI	GTGCAC	1 cut(s)	4020
Asp700I	GAANNNNTTC	3 cut(s)	2783, 3785, 4876
Asp718I	GGTACC	1 cut(s)	125
AspLEI	GCGC	7 cut(s)	174, 1590, 2634, 2642, 2809, 4738, 4975
AspS9I	GGNCC	8 cut(s)	32, 594, 1248, 2247, 2866, 3854, 3949, 4788
AsuII	TTCGAA	2 cut(s)	2605, 2792
AvaI	CYCGRG	3 cut(s)	2925, 4203, 5041
AvaII	GGWCC	5 cut(s)	1248, 2866, 3854, 3949, 4788
AxyI	CCTNAGG	1 cut(s)	36
BaeGI	GKGCMC	2 cut(s)	391, 4024
BalI	TGGCCA	3 cut(s)	673, 1309, 2548
BamHI	GGATCC	1 cut(s)	229
BanI	GGYRCC	2 cut(s)	125, 2806
BanII	GRGCYC	5 cut(s)	45, 421, 3193, 4988, 5533
BauI	CACGAG	5 cut(s)	177, 657, 3186, 3195, 4847
BbsI	GAAGAC	5 cut(s)	4011, 4171, 4534, 4735, 5576
Bbv12I	GWGCWC	7 cut(s)	378, 421, 456, 468, 2139, 3193, 4024
BceAI	ACGGC	5 cut(s)	68, 459, 845, 4713, 5706
BcgI	CGANNNNNNTGC	6 cut(s)	539, 573, 4838, 4872, 4941, 4975
BclI	TGATCA	4 cut(s)	97, 394, 1491, 3823
BfaI	CTAG	7 cut(s)	318, 528, 1499, 2526, 3912, 4679, 5607
BfmI	CTRYAG	8 cut(s)	153, 636, 1481, 2045, 2397, 3238, 4467, 4867
BfoI	RGCGCY	3 cut(s)	175, 2810, 4739
BfrI	CTTAAG	1 cut(s)	4601
BfuAI	ACCTGC	1 cut(s)	2824
BglI	GCCNNNNNGGC	1 cut(s)	1484
BglII	AGATCT	3 cut(s)	2470, 3321, 3619
BlpI	GCTNAGC	2 cut(s)	420, 2133
Bme18I	GGWCC	5 cut(s)	1248, 2866, 3854, 3949, 4788
BmeRI	GACNNNNNGTC	1 cut(s)	4119
BmeT110I	CYCGRG	3 cut(s)	2925, 4203, 5041
BmgT120I	GGNCC	8 cut(s)	32, 594, 1248, 2247, 2866, 3854, 3949, 4788
BmrI	ACTGGG	2 cut(s)	1720, 5083
BmsI	GCATC	8 cut(s)	1116, 1950, 2925, 4221, 4432, 5176, 5307, 5445
BmuI	ACTGGG	2 cut(s)	1720, 5083
BpiI	GAAGAC	5 cut(s)	4011, 4171, 4534, 4735, 5576
BplI	GAGNNNNNCTC	2 cut(s)	3909, 3941
BpmI	CTGGAG	4 cut(s)	468, 3024, 3911, 5714
Bpu10I	CCTNAGC	1 cut(s)	2288
Bpu1102I	GCTNAGC	2 cut(s)	420, 2133
Bpu14I	TTCGAA	2 cut(s)	2605, 2792
BpuEI	CTTGAG	6 cut(s)	638, 930, 939, 1543, 1658, 5336
BsaAI	YACGTR	2 cut(s)	2328, 4019
BsaBI	GATNNNNATC	1 cut(s)	1326
BsaHI	GRCGYC	2 cut(s)	2807, 4616
BsaI	GGTCTC	3 cut(s)	1091, 3672, 5732
BsaWI	WCCGGW	4 cut(s)	704, 1115, 2987, 3681
BsaXI	ACNNNNNCTCC	4 cut(s)	118, 148, 2139, 2169
Bse118I	RCCGGY	1 cut(s)	5750
Bse21I	CCTNAGG	1 cut(s)	36
Bse3DI	GCAATG	3 cut(s)	129, 1494, 3117
Bse8I	GATNNNNATC	1 cut(s)	1326
BseAI	TCCGGA	1 cut(s)	2987
BseGI	GGATG	7 cut(s)	1965, 3121, 4308, 4791, 5083, 5460, 5531
BseJI	GATNNNNATC	1 cut(s)	1326
BseMI	GCAATG	3 cut(s)	129, 1494, 3117
BseRI	GAGGAG	6 cut(s)	635, 1788, 3183, 3581, 3930, 5121
BseSI	GKGCMC	2 cut(s)	391, 4024
BseYI	CCCAGC	3 cut(s)	590, 2869, 5136
BsgI	GTGCAG	1 cut(s)	3797
Bsh1236I	CGCG	1 cut(s)	5663
BshNI	GGYRCC	2 cut(s)	125, 2806
BsiHKAI	GWGCWC	7 cut(s)	378, 421, 456, 468, 2139, 3193, 4024
BsiHKCI	CYCGRG	3 cut(s)	2925, 4203, 5041
BsiSI	CCGG	6 cut(s)	113, 705, 1116, 2988, 3682, 5751
BslFI	GGGAC	2 cut(s)	308, 3450
BsmBI	CGTCTC	2 cut(s)	43, 5683
BsmFI	GGGAC	2 cut(s)	308, 3450
BsmI	GAATGC	7 cut(s)	1046, 1222, 2030, 2088, 2289, 2678, 4514
Bso31I	GGTCTC	3 cut(s)	1091, 3672, 5732
BsoBI	CYCGRG	3 cut(s)	2925, 4203, 5041
Bsp119I	TTCGAA	2 cut(s)	2605, 2792
Bsp13I	TCCGGA	1 cut(s)	2987
Bsp1407I	TGTACA	1 cut(s)	3594
Bsp1720I	GCTNAGC	2 cut(s)	420, 2133
Bsp19I	CCATGG	2 cut(s)	1081, 4009
BspACI	CCGC	9 cut(s)	633, 970, 2417, 2864, 3355, 3481, 3768, 4441, 5221
BspEI	TCCGGA	1 cut(s)	2987
BspFNI	CGCG	1 cut(s)	5663
BspHI	TCATGA	4 cut(s)	22, 45, 100, 4300
BspMAI	CTGCAG	3 cut(s)	157, 1485, 2401
BspMI	ACCTGC	1 cut(s)	2824
BspT104I	TTCGAA	2 cut(s)	2605, 2792
BspT107I	GGYRCC	2 cut(s)	125, 2806
BspTI	CTTAAG	1 cut(s)	4601
BspTNI	GGTCTC	3 cut(s)	1091, 3672, 5732
BsrBI	CCGCTC	1 cut(s)	3355
BsrDI	GCAATG	3 cut(s)	129, 1494, 3117
BsrFI	RCCGGY	1 cut(s)	5750
BsrGI	TGTACA	1 cut(s)	3594
BssAI	RCCGGY	1 cut(s)	5750
BssNAI	GTATAC	2 cut(s)	1992, 3307
BssNI	GRCGYC	2 cut(s)	2807, 4616
BssSI	CACGAG	5 cut(s)	177, 657, 3186, 3195, 4847
BssT1I	CCWWGG	7 cut(s)	121, 1081, 2706, 4009, 4365, 5268, 5601
Bst1107I	GTATAC	2 cut(s)	1992, 3307
Bst2BI	CACGAG	5 cut(s)	177, 657, 3186, 3195, 4847
Bst6I	CTCTTC	7 cut(s)	1948, 2353, 3987, 4321, 4551, 5148, 5708
BstACI	GRCGYC	2 cut(s)	2807, 4616
BstAFI	CTTAAG	1 cut(s)	4601
BstAPI	GCANNNNNTGC	1 cut(s)	3023
BstAUI	TGTACA	1 cut(s)	3594
BstBAI	YACGTR	2 cut(s)	2328, 4019
BstBI	TTCGAA	2 cut(s)	2605, 2792
BstC8I	GCNNGC	6 cut(s)	1307, 1485, 2562, 2566, 3024, 3483
BstDSI	CCRYGG	5 cut(s)	1081, 4009, 5481, 5520, 5722
BstEII	GGTNACC	1 cut(s)	2166
BstF5I	GGATG	7 cut(s)	1965, 3121, 4308, 4791, 5083, 5460, 5531
BstFNI	CGCG	1 cut(s)	5663
BstH2I	RGCGCY	3 cut(s)	175, 2810, 4739
BstHHI	GCGC	7 cut(s)	174, 1590, 2634, 2642, 2809, 4738, 4975
BstNSI	RCATGY	1 cut(s)	2696
BstPI	GGTNACC	1 cut(s)	2166
BstSFI	CTRYAG	8 cut(s)	153, 636, 1481, 2045, 2397, 3238, 4467, 4867
BstSLI	GKGCMC	2 cut(s)	391, 4024
BstSNI	TACGTA	1 cut(s)	2328
BstUI	CGCG	1 cut(s)	5663
BstV2I	GAAGAC	5 cut(s)	4011, 4171, 4534, 4735, 5576
BstXI	CCANNNNNNTGG	2 cut(s)	446, 3336
BstZ17I	GTATAC	2 cut(s)	1992, 3307
Bsu36I	CCTNAGG	1 cut(s)	36
BtgI	CCRYGG	5 cut(s)	1081, 4009, 5481, 5520, 5722
BtgZI	GCGATG	1 cut(s)	4625
BtsCI	GGATG	7 cut(s)	1965, 3121, 4308, 4791, 5083, 5460, 5531
BtsI	GCAGTG	5 cut(s)	2394, 3618, 4069, 5216, 5343
BveI	ACCTGC	1 cut(s)	2824
Cac8I	GCNNGC	6 cut(s)	1307, 1485, 2562, 2566, 3024, 3483
CaiI	CAGNNNCTG	2 cut(s)	5503, 5654
CciI	TCATGA	4 cut(s)	22, 45, 100, 4300
CfoI	GCGC	7 cut(s)	174, 1590, 2634, 2642, 2809, 4738, 4975
Cfr10I	RCCGGY	1 cut(s)	5750
Cfr13I	GGNCC	8 cut(s)	32, 594, 1248, 2247, 2866, 3854, 3949, 4788
CseI	GACGC	4 cut(s)	1398, 1887, 3023, 4605
CsiI	ACCWGGT	2 cut(s)	2311, 2331
CspCI	CAANNNNNGTGG	4 cut(s)	2538, 2573, 4015, 4050
DinI	GGCGCC	1 cut(s)	2808
DraIII	CACNNNGTG	1 cut(s)	4894
DriI	GACNNNNNGTC	1 cut(s)	4119
EaeI	YGGCCR	4 cut(s)	53, 671, 1307, 2546
Eam1104I	CTCTTC	7 cut(s)	1948, 2353, 3987, 4321, 4551, 5148, 5708
Eam1105I	GACNNNNNGTC	1 cut(s)	4119
EarI	CTCTTC	7 cut(s)	1948, 2353, 3987, 4321, 4551, 5148, 5708
EciI	GGCGGA	1 cut(s)	3783
Ecl136II	GAGCTC	2 cut(s)	419, 3191
Eco105I	TACGTA	1 cut(s)	2328
Eco130I	CCWWGG	7 cut(s)	121, 1081, 2706, 4009, 4365, 5268, 5601
Eco147I	AGGCCT	1 cut(s)	5600
Eco24I	GRGCYC	5 cut(s)	45, 421, 3193, 4988, 5533
Eco31I	GGTCTC	3 cut(s)	1091, 3672, 5732
Eco32I	GATATC	3 cut(s)	4252, 4350, 5544
Eco47I	GGWCC	5 cut(s)	1248, 2866, 3854, 3949, 4788
Eco53kI	GAGCTC	2 cut(s)	419, 3191
Eco57I	CTGAAG	7 cut(s)	1580, 2433, 2502, 3202, 3479, 4712, 5522
Eco81I	CCTNAGG	1 cut(s)	36
Eco88I	CYCGRG	3 cut(s)	2925, 4203, 5041
Eco91I	GGTNACC	1 cut(s)	2166
EcoICRI	GAGCTC	2 cut(s)	419, 3191
EcoO109I	RGGNCCY	3 cut(s)	32, 594, 2247
EcoO65I	GGTNACC	1 cut(s)	2166
EcoRI	GAATTC	2 cut(s)	1891, 2779
EcoRV	GATATC	3 cut(s)	4252, 4350, 5544
EcoT14I	CCWWGG	7 cut(s)	121, 1081, 2706, 4009, 4365, 5268, 5601
EcoT22I	ATGCAT	3 cut(s)	208, 1965, 4516
EcoT38I	GRGCYC	5 cut(s)	45, 421, 3193, 4988, 5533
EgeI	GGCGCC	1 cut(s)	2808
EheI	GGCGCC	1 cut(s)	2808
ErhI	CCWWGG	7 cut(s)	121, 1081, 2706, 4009, 4365, 5268, 5601
Esp3I	CGTCTC	2 cut(s)	43, 5683
FalI	AAGNNNNNCTT	6 cut(s)	2768, 2800, 3954, 3986, 5154, 5186
FaqI	GGGAC	2 cut(s)	308, 3450
FauI	CCCGC	1 cut(s)	3474
FauNDI	CATATG	1 cut(s)	2636
FbaI	TGATCA	4 cut(s)	97, 394, 1491, 3823
FblI	GTMKAC	3 cut(s)	1991, 3255, 3306
FokI	GGATG	7 cut(s)	1972, 3128, 4295, 4778, 5090, 5467, 5538
FriOI	GRGCYC	5 cut(s)	45, 421, 3193, 4988, 5533
FspBI	CTAG	7 cut(s)	318, 528, 1499, 2526, 3912, 4679, 5607
FspI	TGCGCA	1 cut(s)	2641
GlaI	GCGC	7 cut(s)	173, 1589, 2633, 2641, 2808, 4737, 4974
GsaI	CCCAGC	3 cut(s)	594, 2873, 5140
GsuI	CTGGAG	4 cut(s)	468, 3024, 3911, 5714
HaeII	RGCGCY	3 cut(s)	175, 2810, 4739
HapII	CCGG	6 cut(s)	113, 705, 1116, 2988, 3682, 5751
HgaI	GACGC	4 cut(s)	1398, 1887, 3023, 4605
HhaI	GCGC	7 cut(s)	174, 1590, 2634, 2642, 2809, 4738, 4975
Hin1I	GRCGYC	2 cut(s)	2807, 4616
Hin6I	GCGC	7 cut(s)	172, 1588, 2632, 2640, 2807, 4736, 4973
HinP1I	GCGC	7 cut(s)	172, 1588, 2632, 2640, 2807, 4736, 4973
HincII	GTYRAC	2 cut(s)	478, 3715
HindII	GTYRAC	2 cut(s)	478, 3715
HindIII	AAGCTT	2 cut(s)	3491, 4221
HpaI	GTTAAC	2 cut(s)	478, 3715
HpaII	CCGG	6 cut(s)	113, 705, 1116, 2988, 3682, 5751
Hpy99I	CGWCG	1 cut(s)	1881
HpyCH4IV	ACGT	5 cut(s)	922, 1611, 2327, 4018, 5740
HpySE526I	ACGT	5 cut(s)	922, 1611, 2327, 4018, 5740
Hsp92I	GRCGYC	2 cut(s)	2807, 4616
HspAI	GCGC	7 cut(s)	172, 1588, 2632, 2640, 2807, 4736, 4973
KasI	GGCGCC	1 cut(s)	2806
Kpn2I	TCCGGA	1 cut(s)	2987
KpnI	GGTACC	1 cut(s)	129
Ksp22I	TGATCA	4 cut(s)	97, 394, 1491, 3823
KspAI	GTTAAC	2 cut(s)	478, 3715
LmnI	GCTCC	7 cut(s)	139, 511, 1747, 2147, 3196, 3352, 4906
LweI	GCATC	8 cut(s)	1116, 1950, 2925, 4221, 4432, 5176, 5307, 5445
MabI	ACCWGGT	2 cut(s)	2311, 2331
MaeI	CTAG	7 cut(s)	318, 528, 1499, 2526, 3912, 4679, 5607
MaeII	ACGT	5 cut(s)	922, 1611, 2327, 4018, 5740
MbiI	CCGCTC	1 cut(s)	3355
MfeI	CAATTG	2 cut(s)	4634, 5255
MlsI	TGGCCA	3 cut(s)	673, 1309, 2548
MluNI	TGGCCA	3 cut(s)	673, 1309, 2548
Mly113I	GGCGCC	1 cut(s)	2807
MlyI	GAGTC	8 cut(s)	20, 814, 1276, 1449, 2917, 3223, 4137, 4952
MmeI	TCCRAC	7 cut(s)	207, 1275, 1930, 2827, 3146, 4766, 5761
Mox20I	TGGCCA	3 cut(s)	673, 1309, 2548
Mph1103I	ATGCAT	3 cut(s)	208, 1965, 4516
MroI	TCCGGA	1 cut(s)	2987
MroXI	GAANNNNTTC	3 cut(s)	2783, 3785, 4876
MscI	TGGCCA	3 cut(s)	673, 1309, 2548
MslI	CAYNNNNRTG	6 cut(s)	77, 2142, 2888, 4173, 4779, 5303
Msp20I	TGGCCA	3 cut(s)	673, 1309, 2548
MspA1I	CMGCKG	3 cut(s)	524, 635, 970
MspCI	CTTAAG	1 cut(s)	4601
MspI	CCGG	6 cut(s)	113, 705, 1116, 2988, 3682, 5751
MunI	CAATTG	2 cut(s)	4634, 5255
Mva1269I	GAATGC	7 cut(s)	1046, 1222, 2030, 2088, 2289, 2678, 4514
MvnI	CGCG	1 cut(s)	5663
NarI	GGCGCC	1 cut(s)	2807
NcoI	CCATGG	2 cut(s)	1081, 4009
NdeI	CATATG	1 cut(s)	2636
NmuCI	GTSAC	8 cut(s)	1929, 2157, 2392, 2480, 2920, 4111, 4291, 4617
NsbI	TGCGCA	1 cut(s)	2641
NsiI	ATGCAT	3 cut(s)	208, 1965, 4516
NspI	RCATGY	1 cut(s)	2696
NspV	TTCGAA	2 cut(s)	2605, 2792
OliI	CACNNNNGTG	2 cut(s)	77, 2888
PaeR7I	CTCGAG	1 cut(s)	2925
PagI	TCATGA	4 cut(s)	22, 45, 100, 4300
PceI	AGGCCT	1 cut(s)	5600
PciI	ACATGT	1 cut(s)	2692
PcsI	WCGNNNNNNNCGW	2 cut(s)	58, 3193
PctI	GAATGC	7 cut(s)	1046, 1222, 2030, 2088, 2289, 2678, 4514
PdmI	GAANNNNTTC	3 cut(s)	2783, 3785, 4876
PflFI	GACNNNGTC	1 cut(s)	761
PflMI	CCANNNNNTGG	4 cut(s)	239, 1066, 3958, 5526
PfoI	TCCNGGA	1 cut(s)	1605
PleI	GAGTC	8 cut(s)	20, 814, 1276, 1449, 2917, 3223, 4136, 4952
PluTI	GGCGCC	1 cut(s)	2810
PpsI	GAGTC	8 cut(s)	20, 814, 1276, 1449, 2917, 3223, 4136, 4952
Ppu21I	YACGTR	2 cut(s)	2328, 4019
PscI	ACATGT	1 cut(s)	2692
Psp124BI	GAGCTC	2 cut(s)	421, 3193
PspEI	GGTNACC	1 cut(s)	2166
PspFI	CCCAGC	3 cut(s)	590, 2869, 5136
PspPI	GGNCC	8 cut(s)	32, 594, 1248, 2247, 2866, 3854, 3949, 4788
PstI	CTGCAG	3 cut(s)	157, 1485, 2401
PstNI	CAGNNNCTG	2 cut(s)	5503, 5654
PsyI	GACNNNGTC	1 cut(s)	761
PvuII	CAGCTG	1 cut(s)	524
RseI	CAYNNNNRTG	6 cut(s)	77, 2142, 2888, 4173, 4779, 5303
SacI	GAGCTC	2 cut(s)	421, 3193
Sau96I	GGNCC	8 cut(s)	32, 594, 1248, 2247, 2866, 3854, 3949, 4788
SbfI	CCTGCAGG	1 cut(s)	1485
SchI	GAGTC	8 cut(s)	20, 814, 1276, 1449, 2917, 3223, 4137, 4952
SdaI	CCTGCAGG	1 cut(s)	1485
SexAI	ACCWGGT	2 cut(s)	2311, 2331
SfaNI	GCATC	8 cut(s)	1116, 1950, 2925, 4221, 4432, 5176, 5307, 5445
SfcI	CTRYAG	8 cut(s)	153, 636, 1481, 2045, 2397, 3238, 4467, 4867
SfoI	GGCGCC	1 cut(s)	2808
Sfr274I	CTCGAG	1 cut(s)	2925
SfuI	TTCGAA	2 cut(s)	2605, 2792
SgrAI	CRCCGGYG	1 cut(s)	5750
SinI	GGWCC	5 cut(s)	1248, 2866, 3854, 3949, 4788
SlaI	CTCGAG	1 cut(s)	2925
SmiMI	CAYNNNNRTG	6 cut(s)	77, 2142, 2888, 4173, 4779, 5303
SmlI	CTYRAG	8 cut(s)	617, 945, 954, 1558, 1673, 2925, 4601, 5351
SmoI	CTYRAG	8 cut(s)	617, 945, 954, 1558, 1673, 2925, 4601, 5351
SnaBI	TACGTA	1 cut(s)	2328
Sse8387I	CCTGCAGG	1 cut(s)	1485
SseBI	AGGCCT	1 cut(s)	5600
SsiI	CCGC	9 cut(s)	633, 970, 2417, 2864, 3355, 3481, 3768, 4441, 5221
SspDI	GGCGCC	1 cut(s)	2806
SspI	AATATT	6 cut(s)	368, 1024, 1126, 1274, 1669, 3531
SspMI	CTAG	7 cut(s)	318, 528, 1499, 2526, 3912, 4679, 5607
SstI	GAGCTC	2 cut(s)	421, 3193
StuI	AGGCCT	1 cut(s)	5600
StyI	CCWWGG	7 cut(s)	121, 1081, 2706, 4009, 4365, 5268, 5601
TaiI	ACGT	5 cut(s)	925, 1614, 2330, 4021, 5743
TaqII	GACCGA	2 cut(s)	1467, 5581
TatI	WGTACW	8 cut(s)	14, 1824, 1840, 3594, 4305, 4812, 5619, 5656
TauI	GCSGC	3 cut(s)	635, 2420, 3358
TseFI	GTSAC	8 cut(s)	1929, 2157, 2392, 2480, 2920, 4111, 4291, 4617
Tsp45I	GTSAC	8 cut(s)	1929, 2157, 2392, 2480, 2920, 4111, 4291, 4617
TspGWI	ACGGA	5 cut(s)	1217, 3222, 3719, 4398, 4615
Tth111I	GACNNNGTC	1 cut(s)	761
Van91I	CCANNNNNTGG	4 cut(s)	239, 1066, 3958, 5526
Vha464I	CTTAAG	1 cut(s)	4601
VneI	GTGCAC	1 cut(s)	4020
VpaK11BI	GGWCC	5 cut(s)	1248, 2866, 3854, 3949, 4788
XceI	RCATGY	1 cut(s)	2696
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2309
XhoI	CTCGAG	1 cut(s)	2925
XmiI	GTMKAC	3 cut(s)	1991, 3255, 3306
XmnI	GAANNNNTTC	3 cut(s)	2783, 3785, 4876
XspI	CTAG	7 cut(s)	318, 528, 1499, 2526, 3912, 4679, 5607
Zsp2I	ATGCAT	3 cut(s)	208, 1965, 4516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.