RLG00000018588

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007588
Physical Location & Seq
Reverse (-)
32248185 .. 32252022
3838 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018588

Sequence Viewer

Length: 1029 bp
ATGACGTTTTCGACGGAGGTCTTCGACAACGAGGTCGTCCCCTCCGCCATCGCCCCAATCGCTCCGCTCCTCCGCGTCACCAACGAAGTCGAGCCCGAGCGCCCTCGCGTGGCCTATCTCTGTCGGTTCTATGCGTTCGAGAAGGCGCACAGATTGGATCCCCGCTCCAGTGGCCGTGGTGTGAGGCAGTTTCAAGACCTTGAGAATGCATCAAGTCTTTCTGCTCGAGTTAAAAAGTCTGATGCCAGGGAAATTACAAGCTTCTATCAGCAATATTATGATCATTATGCTAAAGCTGTTCAAGAGGAGCCGGTATACAGAGCTCAAGTGAGTAAAGCATACAATACACTTAACCAGCTTTTTGAAGTACTTTGGGCGGTTAACAAGTCAGAGAAAGTTGAGGCACTTGCTCGTGAGGAAGCCAAGGAGGGCAGCAATGGAAAAGCTCTTCACACAGCTTGGTCATCTGATTTTATCTCTCTTGGTTGGCCCATGCGTGATGATGAAATCTTGTTTCAGGGAATAAATGGCTCTAAAAGGAAATCTGGAAGCACAGGCAAATCATATTTTATTGAAACTCGCACATTTTGGCACATCTTTCGAAGTTTTGATCGAATGTGGACCTTTTATATACTAGCTCTACAGGTTATGCTCATTATTGCATTCAGCAAGGTTTCACTGCTGGATATATTTCAAAAGGATGTCCTAAGAGATCTATCAAGTATTTTTATTACAGCAGCATTTCTTCGCGTCCTTCAAAGTATTCTGGATATGGTTTTGAACTTCCCTGGATATCACAGGTGTCGGTTCACAGATGTCCTGAGAAATATTCTGAAGATAGTTGTTAGTCGTCTTGCATGGGCAGTCATTCTTCCACTTTTTTATGTGCATTCCTTCAAGGATTCCCCTCAACAAATTGCAGATATACTGTCATTCCTTAAGAACATTCAAGGCATTCCATCATTATATTTACCGGCTGTTGCAATTTCTCTTGTGGTGGTCATAGAAGGCTTGATATATCTCATCTGA

Protein Analysis

343

Amino Acids

39.25

Weight (kDa)

9.02

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 20 - 129 4.6e-06 Vta1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
AccBSI CCGCTC 2 cut(s) 67, 165
AccI GTMKAC 1 cut(s) 315
AccII CGCG 3 cut(s) 75, 108, 750
AciI CCGC 5 cut(s) 45, 65, 73, 163, 377
AclWI GGATC 2 cut(s) 152, 165
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 854
AfaI GTAC 1 cut(s) 369
AfiI CCNNNNNNNGG 1 cut(s) 109
AflII CTTAAG 1 cut(s) 938
AgsI TTSAA 9 cut(s) 194, 302, 365, 575, 695, 758, 781, 898, 950
AjnI CCWGG 2 cut(s) 245, 787
AluBI AGCT 7 cut(s) 261, 296, 323, 358, 446, 458, 638
AluI AGCT 7 cut(s) 261, 296, 323, 358, 446, 458, 638
Alw21I GWGCWC 1 cut(s) 325
AlwI GGATC 2 cut(s) 152, 165
Ama87I CYCGRG 2 cut(s) 95, 225
AoxI GGCC 3 cut(s) 111, 172, 488
ApeKI GCWGC 2 cut(s) 432, 737
AspLEI GCGC 2 cut(s) 102, 148
AspS9I GGNCC 2 cut(s) 489, 621
AsuHPI GGTGA 1 cut(s) 70
AsuII TTCGAA 1 cut(s) 601
AvaI CYCGRG 2 cut(s) 95, 225
AvaII GGWCC 1 cut(s) 621
BamHI GGATCC 1 cut(s) 157
BanII GRGCYC 2 cut(s) 96, 325
BauI CACGAG 1 cut(s) 411
BbsI GAAGAC 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 325
BbvI GCAGC 2 cut(s) 444, 749
BccI CCATC 2 cut(s) 56, 967
BceAI ACGGC 1 cut(s) 159
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 2 cut(s) 247, 789
BclI TGATCA 1 cut(s) 280
BfaI CTAG 1 cut(s) 635
BfmI CTRYAG 1 cut(s) 641
BfoI RGCGCY 1 cut(s) 103
BfrI CTTAAG 1 cut(s) 938
BglII AGATCT 1 cut(s) 712
BisI GCNGC 2 cut(s) 433, 738
BlsI GCNGC 2 cut(s) 434, 739
BmcAI AGTACT 1 cut(s) 369
Bme1390I CCNGG 2 cut(s) 247, 789
Bme18I GGWCC 1 cut(s) 621
BmeT110I CYCGRG 2 cut(s) 95, 225
BmgT120I GGNCC 2 cut(s) 489, 621
BmiI GGNNCC 2 cut(s) 159, 309
BmrFI CCNGG 2 cut(s) 247, 789
BmsI GCATC 2 cut(s) 218, 232
BoxI GACNNNNGTC 1 cut(s) 17
BpiI GAAGAC 1 cut(s) 13
BpmI CTGGAG 1 cut(s) 151
Bpu14I TTCGAA 1 cut(s) 601
BpuEI CTTGAG 2 cut(s) 221, 309
BsaJI CCNNGG 4 cut(s) 175, 246, 423, 787
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse118I RCCGGY 2 cut(s) 310, 973
Bse1I ACTGG 1 cut(s) 168
Bse3DI GCAATG 1 cut(s) 442
BseBI CCWGG 2 cut(s) 247, 789
BseDI CCNNGG 4 cut(s) 175, 246, 423, 787
BseGI GGATG 1 cut(s) 706
BseLI CCNNNNNNNGG 1 cut(s) 109
BseMI GCAATG 1 cut(s) 442
BseMII CTCAG 1 cut(s) 812
BseNI ACTGG 1 cut(s) 168
BseRI GAGGAG 2 cut(s) 59, 320
BseXI GCAGC 2 cut(s) 444, 749
Bsh1236I CGCG 3 cut(s) 75, 108, 750
BshFI GGCC 3 cut(s) 113, 174, 490
BsiHKAI GWGCWC 1 cut(s) 325
BsiHKCI CYCGRG 2 cut(s) 95, 225
BsiSI CCGG 2 cut(s) 311, 974
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 23
BsmI GAATGC 4 cut(s) 211, 662, 889, 954
BsnI GGCC 3 cut(s) 113, 174, 490
BsoBI CYCGRG 2 cut(s) 95, 225
Bsp119I TTCGAA 1 cut(s) 601
Bsp1286I GDGCHC 2 cut(s) 96, 325
Bsp143I GATC 4 cut(s) 157, 280, 610, 712
BspACI CCGC 5 cut(s) 45, 65, 73, 163, 377
BspANI GGCC 3 cut(s) 113, 174, 490
BspCNI CTCAG 1 cut(s) 813
BspFNI CGCG 3 cut(s) 75, 108, 750
BspLI GGNNCC 2 cut(s) 159, 309
BspPI GGATC 2 cut(s) 152, 165
BspQI GCTCTTC 1 cut(s) 453
BspT104I TTCGAA 1 cut(s) 601
BspTI CTTAAG 1 cut(s) 938
BsrBI CCGCTC 2 cut(s) 67, 165
BsrDI GCAATG 1 cut(s) 442
BsrFI RCCGGY 2 cut(s) 310, 973
BsrI ACTGG 1 cut(s) 168
BssAI RCCGGY 2 cut(s) 310, 973
BssECI CCNNGG 4 cut(s) 175, 246, 423, 787
BssMI GATC 4 cut(s) 157, 280, 610, 712
BssNAI GTATAC 1 cut(s) 316
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 1 cut(s) 423
Bst1107I GTATAC 1 cut(s) 316
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 2 cut(s) 247, 789
Bst4CI ACNGT 1 cut(s) 930
Bst6I CTCTTC 1 cut(s) 453
BstAFI CTTAAG 1 cut(s) 938
BstBI TTCGAA 1 cut(s) 601
BstDEI CTNAG 2 cut(s) 707, 821
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 1 cut(s) 706
BstFNI CGCG 3 cut(s) 75, 108, 750
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 2 cut(s) 102, 148
BstKTI GATC 4 cut(s) 160, 283, 613, 715
BstMBI GATC 4 cut(s) 157, 280, 610, 712
BstMWI GCNNNNNNNGC 2 cut(s) 59, 171
BstNI CCWGG 2 cut(s) 247, 789
BstPAI GACNNNNGTC 1 cut(s) 17
BstSCI CCNGG 2 cut(s) 245, 787
BstSFI CTRYAG 1 cut(s) 641
BstUI CGCG 3 cut(s) 75, 108, 750
BstV1I GCAGC 2 cut(s) 444, 749
BstV2I GAAGAC 1 cut(s) 13
BstX2I RGATCY 2 cut(s) 157, 712
BstYI RGATCY 2 cut(s) 157, 712
BstZ17I GTATAC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 113, 174, 490
BtgI CCRYGG 1 cut(s) 175
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 1 cut(s) 706
BtsI GCAGTG 1 cut(s) 677
BtsIMutI CAGTG 2 cut(s) 175, 677
CfoI GCGC 2 cut(s) 102, 148
Cfr10I RCCGGY 2 cut(s) 310, 973
Cfr13I GGNCC 2 cut(s) 489, 621
CseI GACGC 2 cut(s) 64, 739
Csp6I GTAC 1 cut(s) 368
CviAII CATG 2 cut(s) 493, 858
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 2 cut(s) 707, 821
DpnI GATC 4 cut(s) 159, 282, 612, 714
DpnII GATC 4 cut(s) 157, 280, 610, 712
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
EciI GGCGGA 1 cut(s) 34
Ecl136II GAGCTC 1 cut(s) 323
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 2 cut(s) 96, 325
Eco32I GATATC 1 cut(s) 794
Eco47I GGWCC 1 cut(s) 621
Eco53kI GAGCTC 1 cut(s) 323
Eco57I CTGAAG 1 cut(s) 854
Eco88I CYCGRG 2 cut(s) 95, 225
EcoICRI GAGCTC 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 245, 787
EcoRV GATATC 1 cut(s) 794
EcoT14I CCWWGG 1 cut(s) 423
EcoT22I ATGCAT 1 cut(s) 211
EcoT38I GRGCYC 2 cut(s) 96, 325
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 2 cut(s) 496, 861
FaqI GGGAC 1 cut(s) 23
FatI CATG 2 cut(s) 492, 857
FauI CCCGC 1 cut(s) 170
FbaI TGATCA 1 cut(s) 280
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 2 cut(s) 433, 738
FokI GGATG 1 cut(s) 713
FriOI GRGCYC 2 cut(s) 96, 325
Fsp4HI GCNGC 2 cut(s) 433, 738
FspBI CTAG 1 cut(s) 635
GlaI GCGC 2 cut(s) 101, 147
GluI GCNGC 2 cut(s) 433, 738
GsuI CTGGAG 1 cut(s) 151
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 3 cut(s) 113, 174, 490
HapII CCGG 2 cut(s) 311, 974
HgaI GACGC 2 cut(s) 64, 739
HhaI GCGC 2 cut(s) 102, 148
Hin1II CATG 2 cut(s) 496, 861
Hin6I GCGC 2 cut(s) 100, 146
HinP1I GCGC 2 cut(s) 100, 146
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HindIII AAGCTT 1 cut(s) 259
HinfI GANTC 1 cut(s) 902
HpaI GTTAAC 1 cut(s) 382
HpaII CCGG 2 cut(s) 311, 974
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 4 cut(s) 316, 382, 621, 810
Hpy188I TCNGA 5 cut(s) 241, 391, 469, 834, 1028
Hpy188III TCNNGA 7 cut(s) 139, 194, 302, 413, 546, 767, 820
Hpy8I GTNNAC 4 cut(s) 316, 382, 621, 810
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 4 cut(s) 136, 764, 904, 1001
HpyCH4III ACNGT 1 cut(s) 930
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 6 cut(s) 209, 662, 857, 889, 920, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 171
HpyF3I CTNAG 2 cut(s) 707, 821
HpySE526I ACGT 1 cut(s) 5
Hsp92II CATG 2 cut(s) 496, 861
HspAI GCGC 2 cut(s) 100, 146
Ksp22I TGATCA 1 cut(s) 280
KspAI GTTAAC 1 cut(s) 382
Kzo9I GATC 4 cut(s) 157, 280, 610, 712
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 4 cut(s) 67, 72, 170, 307
Lsp1109I GCAGC 2 cut(s) 444, 749
LweI GCATC 2 cut(s) 218, 232
MaeI CTAG 1 cut(s) 635
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 1 cut(s) 76
MalI GATC 4 cut(s) 159, 282, 612, 714
MbiI CCGCTC 2 cut(s) 67, 165
MboI GATC 4 cut(s) 157, 280, 610, 712
MboII GAAGA 5 cut(s) 13, 440, 737, 847, 863
MflI RGATCY 2 cut(s) 157, 712
MhlI GDGCHC 2 cut(s) 96, 325
MluCI AATT 3 cut(s) 252, 915, 984
Mph1103I ATGCAT 1 cut(s) 211
MseI TTAA 4 cut(s) 231, 351, 381, 939
MspCI CTTAAG 1 cut(s) 938
MspI CCGG 2 cut(s) 311, 974
MspR9I CCNGG 2 cut(s) 247, 789
Mva1269I GAATGC 4 cut(s) 211, 662, 889, 954
MvaI CCWGG 2 cut(s) 247, 789
MvnI CGCG 3 cut(s) 75, 108, 750
MwoI GCNNNNNNNGC 2 cut(s) 59, 171
NdeII GATC 4 cut(s) 157, 280, 610, 712
NlaIII CATG 2 cut(s) 496, 861
NlaIV GGNNCC 2 cut(s) 159, 309
NmuCI GTSAC 1 cut(s) 76
NsiI ATGCAT 1 cut(s) 211
NspV TTCGAA 1 cut(s) 601
PaeR7I CTCGAG 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 453
PctI GAATGC 4 cut(s) 211, 662, 889, 954
PfeI GAWTC 1 cut(s) 902
PkrI GCNGC 2 cut(s) 434, 739
PshAI GACNNNNGTC 1 cut(s) 17
Psp124BI GAGCTC 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 245, 787
PspGI CCWGG 2 cut(s) 245, 787
PspN4I GGNNCC 2 cut(s) 159, 309
PspPI GGNCC 2 cut(s) 489, 621
PspXI VCTCGAGB 1 cut(s) 225
PsuI RGATCY 2 cut(s) 157, 712
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
SacI GAGCTC 1 cut(s) 325
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 4 cut(s) 231, 351, 381, 939
SatI GCNGC 2 cut(s) 433, 738
Sau3AI GATC 4 cut(s) 157, 280, 610, 712
Sau96I GGNCC 2 cut(s) 489, 621
ScaI AGTACT 1 cut(s) 369
ScrFI CCNGG 2 cut(s) 247, 789
SduI GDGCHC 2 cut(s) 96, 325
SfaNI GCATC 2 cut(s) 218, 232
SfcI CTRYAG 1 cut(s) 641
Sfr274I CTCGAG 1 cut(s) 225
SfuI TTCGAA 1 cut(s) 601
SinI GGWCC 1 cut(s) 621
SlaI CTCGAG 1 cut(s) 225
SmlI CTYRAG 4 cut(s) 200, 225, 324, 938
SmoI CTYRAG 4 cut(s) 200, 225, 324, 938
Sse9I AATT 3 cut(s) 252, 915, 984
SsiI CCGC 5 cut(s) 45, 65, 73, 163, 377
SspI AATATT 2 cut(s) 275, 829
SspMI CTAG 1 cut(s) 635
SstI GAGCTC 1 cut(s) 325
StyD4I CCNGG 2 cut(s) 245, 787
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 1 cut(s) 930
TaiI ACGT 1 cut(s) 8
TaqI TCGA 7 cut(s) 11, 24, 90, 138, 226, 601, 613
TasI AATT 3 cut(s) 252, 915, 984
TatI WGTACW 1 cut(s) 367
TfiI GAWTC 1 cut(s) 902
Tru1I TTAA 4 cut(s) 231, 351, 381, 939
Tru9I TTAA 4 cut(s) 231, 351, 381, 939
TscAI CASTG 2 cut(s) 175, 684
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 2 cut(s) 432, 737
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 1 cut(s) 519
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 2 cut(s) 175, 684
Vha464I CTTAAG 1 cut(s) 938
VpaK11BI GGWCC 1 cut(s) 621
XhoI CTCGAG 1 cut(s) 225
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 1 cut(s) 635
ZrmI AGTACT 1 cut(s) 369
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.