Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007588

Physical Location & Seq

Genomic Coordinates Forward (+)

1322311 .. 1331431

Gene Structure 9121 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1004000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGTCAAACCTGGATCCGCCAGGCCCTAGCGGGCCGCAGGGGCTGACGAGAAGGCCGTCGAGGAGCGCAGCCACCACCACCTTCTCCACTGAGGTGTTCGACAACGAGGTCGTCCCTTCCGCTCTCGCCTCCATCGCTCCCATCCTCCGAGTGGCCAACGAGATCGAGACCGAGCGTCCTCGCGTCGCCTATCTCTGTCGGTTCTATGCGTTCGAGAAGGCGCACCGACTGGATCCGAGCTCCAGCGGCCGTGGCGTCCGCCAGTTCAAGACTTCGCTCCTCCAACGACTCGAGAGGGACAATGCATCAAGTCTGAATTCTCGGGTGAAAAACACAGATGACAGAGAAATTAAAAGCTTCTATCAGCAATATTATAAGCATTATGTCAGAGCTCTTGACCAGGGCGAGCAGGCTGACAGAGCCCAACTGGGAAAAGCCTACCGCACTGCCGGGGTCCTTTTTGAAGTGCTTTGCGCTGTTAACAAGACTGAGAAAGTTGATGAAGTTGCTCCTGAGATCATTGCGGCTGCTAAAGATGTACAAGAAAAGACAGAAATTTATGCTCCCTATAACATTCTTCCTTTGGATTCTGCTGGTGCTACACAGTCTATCATGCAACTTGAAGAGGTTAAGGCTGCAGTCGGTGCACTATTGAACACTCGTGGTTTGAACTGGCCTAGTGCATTGGAGAACAGTCACAAAGCTGGTGATCTCGACCTCCTTGATTGGCTGAGGGCCATGTTTGGATTCCAGGCATGTATAAAAATAACCGATTTCAATGACAACGTCAGGAACCAGAGGGAGCATTTGATATCCCTACTTGCAAATCCTCATATAAGGCTTCATCCCAAACCCGAACCTCTTAATAAGATGGATGATCGAGCTGTTGATAAAGTTATGGGAAAACTTTTTAAGAACTACAAAACGTGGTGCAAATTTTTGGGAAGAAAACATAGTTTGAGGCTTCCCCAAGGTCAGCAAGAAATACAGCAAAGGAAGATACTGTATATGGGTTTGTATCTTCTCATCTGGGGTGAAGCAGCGAATGTCCGCTTTATGCCAGAATGTCTGTGCTATATTTTTCATAATATGGCATATGAACTCCATGGCCTGTTGGCTGGAAATGTCAGCATCGTTACTGGGGAAAATATAAAGCCTTCTTATGGTGGGGATGATGAGGCTTTTTTACGGAAAGTTATAACCCCCTTGTACCGTGTAATTGAAAAGGAAGCCAAGAAGAGCGAAAATGGAAAAGCTCCTCACATAGCTTGGTGCAACTATGACGATCTCAATGAATATTTCTGGTCGTCTGATTGCTTCTCTCTTGGTTGGCCCATGCGTGATGATGGTGATTTTTTCAAATCAACGCGTGACTTGGTGCAGGGAAGAAAGGGCTCTAGAAGAAAATCTGGAAGCACAGGAAAATCGTATTTTATTGAAACTCGGACATTTTGGCACATTTTTCGAAGTTTTGACCGGTTTTGGACCTTTTACATACTGGCTTTACAGGCTATGCTCATTGTTGCTTTTAGAGGGATTTCACCATTGGATATTTTTCAAAAGAATGTCTTGAGAGATCTATCAAGTATTTTCATCACAGCAGCATTTCTTCGCGTCCTTCAAAGCATTTTGGATATTGTTTTGAACTTCCCGGGATATCATAGGTGGAGGTTCACTGATGTGCTGCGAAATATTCTCAAGATAATTGTTAGTCTTGCATGGGCAATCATTCTTCCACTGTTTTATGTGCATTCATTCCAGAATGCCCCTAAACAAGTTATGGATTTACTGTCATTCCTGAAGAATATCAATGGTGTTCCTCCTCTATACCTTATGGCTGTTGCAGTATATTTGCTGCCAAATTTATTAGGAGCAGTTTTGTTCCTTTTCCCGCTGCTCCGACGTTGGATTGAAAACTCAGACTGGCATATCATTAGGTTCCTCTTGTGGTGGTCACAGCCAAGAATCTATGTTGGGAGGGGAATGCATGAAAGTCAATTTGCCCTTATTAAGTACACTATTTTTTGGGTGCTGCTTTTGGCTTGCAAGTTTACAGTTAGCTATCTTATCCAGATAAGGCCGTTGGTGAAGCCAACTAGAGACATTATGAACATTCGTCGTGTAGATTATCAGTGGCATGAATTTTTCCCTAATGCTCAAAACAACTATGGAGCAGTTGTGTCACTCTGGGCACCAGTAGTCTTGGTTTATTTGATGGACACTCAAATATGGTATGCTATTTTTCAGACTTTGTATGGTGGTGTTGTTGGCGCATTCGATCGCCTAGGAGAGATTCGAACACTGGGTATGCTAAGATCAAGGTTCCAGTCTCTACCCGGTGCATTCAACACATACCTAGTGCCTTCAGATAAATCAACAAAAAGGGGATTCTCTTTCTCAAAGCGTTTCGTGGAGATTACAGCAAGCAGGAGAAGTGAAGCTGCAAAGTTTGCTCAGTTGTGGAATGAAGTGATCTGTAGCTTCCGTGAGGAAGATCTCATAAATGACAGGAAATGCTCCCCAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006075 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009555 GO:0009556 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010208 GO:0010769 GO:0010927 GO:0016043 GO:0016049 GO:0016051 GO:0019953 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034293 GO:0034637 GO:0034645 GO:0040007 GO:0040008 GO:0043062 GO:0043170 GO:0043900 GO:0043934 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048236 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048646 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051273 GO:0051274 GO:0051321 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0080092 GO:0085029 GO:1901576 GO:1903046 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

843

Amino Acids

96.96

Weight (kDa)

9.39

Isoelectric Point (pI)

50.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vta1	PF04652	44 - 176	1.5e-15	Vta1 like
FKS1_dom1	PF14288	333 - 444	2.1e-42	1,3-beta-glucan synthase subunit FKS1, domain-1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13680
fragaria_vesca	FvH4_1g24300
malus_domestica	MD01G1004000.v1.1 MD01G1004100.v1.1 MD02G1211500.v1.1
prunus_persica	Prupe.4G271000_v2.0.a1
pyrus_communis	pycom01g02040 pycom15g30380
rosa_chinensis	RchiOBHm_Chr2g0120781
rosa_laevigata	RLG00000018581 RLG00000018588
rosa_roxburghii	Rroxscaffold_2G00122840 Rroxscaffold_2G00122950
rosa_rugosa	Rorug02G0231300
rosa_samantha	Rh2AG287900 Rh2AG288000 Rh2AG288100 Rh2BG299200 Rh2BG299300 Rh2CG277200 Rh2CG277300 Rh2DG313100 Rh2DG313200
rosa_wichuraiana	Rw2G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	375, 1199
AasI	GACNNNNNNGTC	1 cut(s)	107
AccB1I	GGYRCC	1 cut(s)	2191
AccBSI	CCGCTC	1 cut(s)	122
AccII	CGCG	3 cut(s)	183, 1369, 1614
AclWI	GGATC	4 cut(s)	8, 21, 227, 240
AcoI	YGGCCR	2 cut(s)	153, 247
AcsI	RAATTY	5 cut(s)	316, 555, 935, 1861, 2141
AcuI	CTGAAG	2 cut(s)	1820, 2349
AcyI	GRCGYC	1 cut(s)	255
AfaI	GTAC	3 cut(s)	540, 1211, 2015
AfiI	CCNNNNNNNGG	2 cut(s)	151, 1163
AflIII	ACRYGT	1 cut(s)	1367
AgeI	ACCGGT	1 cut(s)	1476
AhdI	GACNNNNNGTC	1 cut(s)	174
AjnI	CCWGG	4 cut(s)	9, 19, 399, 750
AleI	CACNNNNGTG	2 cut(s)	92, 1679
Alw21I	GWGCWC	3 cut(s)	242, 394, 649
Alw26I	GTCTC	3 cut(s)	161, 2094, 2334
Alw44I	GTGCAC	1 cut(s)	645
AlwI	GGATC	4 cut(s)	8, 21, 227, 240
AlwNI	CAGNNNCTG	1 cut(s)	43
Ama87I	CYCGRG	3 cut(s)	290, 321, 1651
ApaLI	GTGCAC	1 cut(s)	645
ApoI	RAATTY	5 cut(s)	316, 555, 935, 1861, 2141
AsiGI	ACCGGT	1 cut(s)	1476
AspA2I	CCTAGG	1 cut(s)	2284
AspLEI	GCGC	4 cut(s)	68, 223, 476, 2273
AspS9I	GGNCC	6 cut(s)	23, 32, 454, 735, 1332, 1485
AsuC2I	CCSGG	4 cut(s)	451, 1652, 1653, 2337
AsuHPI	GGTGA	6 cut(s)	337, 719, 1046, 1361, 1533, 2098
AsuII	TTCGAA	2 cut(s)	1465, 2296
AvaI	CYCGRG	3 cut(s)	290, 321, 1651
AvaII	GGWCC	2 cut(s)	454, 1485
AvrII	CCTAGG	1 cut(s)	2284
BaeGI	GKGCMC	2 cut(s)	649, 2194
BalI	TGGCCA	1 cut(s)	155
BamHI	GGATCC	2 cut(s)	13, 232
BanI	GGYRCC	1 cut(s)	2191
BanII	GRGCYC	4 cut(s)	242, 394, 424, 1397
BauI	CACGAG	1 cut(s)	660
Bbv12I	GWGCWC	3 cut(s)	242, 394, 649
BbvCI	CCTCAGC	1 cut(s)	731
BccI	CCATC	5 cut(s)	140, 149, 865, 1340, 2209
BceAI	ACGGC	3 cut(s)	40, 234, 2065
BcgI	CGANNNNNNTGC	2 cut(s)	1445, 1479
BciT130I	CCWGG	4 cut(s)	11, 21, 401, 752
BcnI	CCSGG	4 cut(s)	451, 1652, 1653, 2337
BcoDI	GTCTC	3 cut(s)	161, 2094, 2334
BfaI	CTAG	6 cut(s)	27, 678, 1398, 2097, 2285, 2357
BfmI	CTRYAG	2 cut(s)	636, 2476
BglI	GCCNNNNNGGC	1 cut(s)	40
BglII	AGATCT	2 cut(s)	1576, 2494
BlnI	CCTAGG	1 cut(s)	2284
Bme1390I	CCNGG	8 cut(s)	11, 21, 401, 451, 752, 1652, 1653, 2337
Bme18I	GGWCC	2 cut(s)	454, 1485
BmeRI	GACNNNNNGTC	1 cut(s)	174
BmeT110I	CYCGRG	3 cut(s)	290, 321, 1651
BmgT120I	GGNCC	6 cut(s)	23, 32, 454, 735, 1332, 1485
BmiI	GGNNCC	7 cut(s)	15, 234, 455, 794, 1940, 2193, 2324
BmrFI	CCNGG	8 cut(s)	11, 21, 401, 451, 752, 1652, 1653, 2337
BmrI	ACTGGG	3 cut(s)	437, 1149, 2312
BmsI	GCATC	2 cut(s)	314, 1140
BmuI	ACTGGG	3 cut(s)	437, 1149, 2312
BpmI	CTGGAG	1 cut(s)	226
Bpu10I	CCTNAGC	1 cut(s)	731
Bpu14I	TTCGAA	2 cut(s)	1465, 2296
BpuEI	CTTGAG	2 cut(s)	1591, 1682
BpuMI	CCSGG	4 cut(s)	451, 1652, 1653, 2337
BsaHI	GRCGYC	1 cut(s)	255
BsaI	GGTCTC	1 cut(s)	161
BsaJI	CCNNGG	7 cut(s)	250, 400, 450, 970, 1105, 1651, 2284
BsaWI	WCCGGW	1 cut(s)	1476
BsaXI	ACNNNNNCTCC	2 cut(s)	2163, 2193
Bsc4I	CCNNNNNNNGG	2 cut(s)	151, 1163
Bse118I	RCCGGY	1 cut(s)	1476
Bse3DI	GCAATG	1 cut(s)	519
BseBI	CCWGG	4 cut(s)	11, 21, 401, 752
BseDI	CCNNGG	7 cut(s)	250, 400, 450, 970, 1105, 1651, 2284
BseGI	GGATG	4 cut(s)	141, 844, 880, 1177
BseLI	CCNNNNNNNGG	2 cut(s)	151, 1163
BseMI	GCAATG	1 cut(s)	519
BseMII	CTCAG	6 cut(s)	81, 480, 504, 722, 1932, 2468
BseRI	GAGGAG	4 cut(s)	76, 269, 1248, 1812
BseSI	GKGCMC	2 cut(s)	649, 2194
BseX3I	CGGCCG	1 cut(s)	247
BsgI	GTGCAG	1 cut(s)	1400
Bsh1236I	CGCG	3 cut(s)	183, 1369, 1614
Bsh1285I	CGRYCG	2 cut(s)	250, 2281
BshNI	GGYRCC	1 cut(s)	2191
BshTI	ACCGGT	1 cut(s)	1476
BsiEI	CGRYCG	2 cut(s)	250, 2281
BsiHKAI	GWGCWC	3 cut(s)	242, 394, 649
BsiHKCI	CYCGRG	3 cut(s)	290, 321, 1651
BsiSI	CCGG	4 cut(s)	450, 1477, 1652, 2337
BslFI	GGGAC	2 cut(s)	98, 311
BslI	CCNNNNNNNGG	2 cut(s)	151, 1163
BsmAI	GTCTC	3 cut(s)	161, 2094, 2334
BsmFI	GGGAC	2 cut(s)	98, 311
BsmI	GAATGC	5 cut(s)	1750, 1768, 1989, 2273, 2342
Bso31I	GGTCTC	1 cut(s)	161
BsoBI	CYCGRG	3 cut(s)	290, 321, 1651
Bsp119I	TTCGAA	2 cut(s)	1465, 2296
Bsp1286I	GDGCHC	6 cut(s)	242, 394, 424, 649, 1397, 2194
Bsp1407I	TGTACA	1 cut(s)	538
Bsp19I	CCATGG	1 cut(s)	1105
BspCNI	CTCAG	6 cut(s)	82, 481, 505, 723, 1931, 2467
BspFNI	CGCG	3 cut(s)	183, 1369, 1614
BspLI	GGNNCC	7 cut(s)	15, 234, 455, 794, 1940, 2193, 2324
BspMAI	CTGCAG	1 cut(s)	640
BspPI	GGATC	4 cut(s)	8, 21, 227, 240
BspQI	GCTCTTC	1 cut(s)	1232
BspT104I	TTCGAA	2 cut(s)	1465, 2296
BspT107I	GGYRCC	1 cut(s)	2191
BspTNI	GGTCTC	1 cut(s)	161
BsrBI	CCGCTC	1 cut(s)	122
BsrDI	GCAATG	1 cut(s)	519
BsrFI	RCCGGY	1 cut(s)	1476
BsrGI	TGTACA	1 cut(s)	538
BssAI	RCCGGY	1 cut(s)	1476
BssECI	CCNNGG	7 cut(s)	250, 400, 450, 970, 1105, 1651, 2284
BssNI	GRCGYC	1 cut(s)	255
BssSI	CACGAG	1 cut(s)	660
BssT1I	CCWWGG	3 cut(s)	970, 1105, 2284
Bst2BI	CACGAG	1 cut(s)	660
Bst2UI	CCWGG	4 cut(s)	11, 21, 401, 752
Bst4CI	ACNGT	7 cut(s)	606, 695, 1005, 1214, 1740, 1791, 2056
Bst6I	CTCTTC	2 cut(s)	618, 1232
BstACI	GRCGYC	1 cut(s)	255
BstAPI	GCANNNNNTGC	2 cut(s)	644, 2450
BstAUI	TGTACA	1 cut(s)	538
BstBI	TTCGAA	2 cut(s)	1465, 2296
BstC8I	GCNNGC	5 cut(s)	32, 407, 411, 2044, 2425
BstDEI	CTNAG	7 cut(s)	90, 489, 513, 731, 1918, 2312, 2454
BstDSI	CCRYGG	2 cut(s)	250, 1105
BstF5I	GGATG	4 cut(s)	141, 844, 880, 1177
BstFNI	CGCG	3 cut(s)	183, 1369, 1614
BstHHI	GCGC	4 cut(s)	68, 223, 476, 2273
BstMAI	GTCTC	3 cut(s)	161, 2094, 2334
BstMCI	CGRYCG	2 cut(s)	250, 2281
BstMWI	GCNNNNNNNGC	8 cut(s)	40, 134, 246, 252, 419, 644, 1508, 2450
BstNI	CCWGG	4 cut(s)	11, 21, 401, 752
BstNSI	RCATGY	1 cut(s)	759
BstSCI	CCNGG	8 cut(s)	9, 19, 399, 449, 750, 1650, 1651, 2335
BstSFI	CTRYAG	2 cut(s)	636, 2476
BstSLI	GKGCMC	2 cut(s)	649, 2194
BstUI	CGCG	3 cut(s)	183, 1369, 1614
BstX2I	RGATCY	4 cut(s)	13, 232, 1576, 2494
BstYI	RGATCY	4 cut(s)	13, 232, 1576, 2494
BstZI	CGGCCG	1 cut(s)	247
BtgI	CCRYGG	2 cut(s)	250, 1105
BtgZI	GCGATG	1 cut(s)	118
BtsCI	GGATG	4 cut(s)	141, 844, 880, 1177
BtsI	GCAGTG	1 cut(s)	444
BtsIMutI	CAGTG	6 cut(s)	87, 444, 1674, 1736, 2138, 2300
Cac8I	GCNNGC	5 cut(s)	32, 407, 411, 2044, 2425
CaiI	CAGNNNCTG	1 cut(s)	43
CfoI	GCGC	4 cut(s)	68, 223, 476, 2273
Cfr10I	RCCGGY	1 cut(s)	1476
Cfr13I	GGNCC	6 cut(s)	23, 32, 454, 735, 1332, 1485
Cfr9I	CCCGGG	1 cut(s)	1651
CseI	GACGC	4 cut(s)	164, 172, 244, 1603
Csp6I	GTAC	3 cut(s)	539, 1210, 2014
CspAI	ACCGGT	1 cut(s)	1476
CviAII	CATG	8 cut(s)	613, 739, 756, 1106, 1336, 1719, 1988, 2138
CviQI	GTAC	3 cut(s)	539, 1210, 2014
DdeI	CTNAG	7 cut(s)	90, 489, 513, 731, 1918, 2312, 2454
DrdI	GACNNNNNNGTC	1 cut(s)	107
DriI	GACNNNNNGTC	1 cut(s)	174
DseDI	GACNNNNNNGTC	1 cut(s)	107
EaeI	YGGCCR	2 cut(s)	153, 247
EagI	CGGCCG	1 cut(s)	247
Eam1104I	CTCTTC	2 cut(s)	618, 1232
Eam1105I	GACNNNNNGTC	1 cut(s)	174
EarI	CTCTTC	2 cut(s)	618, 1232
EciI	GGCGGA	2 cut(s)	6, 248
Ecl136II	GAGCTC	2 cut(s)	240, 392
EclXI	CGGCCG	1 cut(s)	247
Eco130I	CCWWGG	3 cut(s)	970, 1105, 2284
Eco24I	GRGCYC	4 cut(s)	242, 394, 424, 1397
Eco31I	GGTCTC	1 cut(s)	161
Eco32I	GATATC	2 cut(s)	813, 1658
Eco47I	GGWCC	2 cut(s)	454, 1485
Eco52I	CGGCCG	1 cut(s)	247
Eco53kI	GAGCTC	2 cut(s)	240, 392
Eco57I	CTGAAG	2 cut(s)	1820, 2349
Eco88I	CYCGRG	3 cut(s)	290, 321, 1651
EcoICRI	GAGCTC	2 cut(s)	240, 392
EcoO109I	RGGNCCY	2 cut(s)	23, 454
EcoRI	GAATTC	1 cut(s)	316
EcoRII	CCWGG	4 cut(s)	9, 19, 399, 750
EcoRV	GATATC	2 cut(s)	813, 1658
EcoT14I	CCWWGG	3 cut(s)	970, 1105, 2284
EcoT22I	ATGCAT	2 cut(s)	307, 1989
EcoT38I	GRGCYC	4 cut(s)	242, 394, 424, 1397
ErhI	CCWWGG	3 cut(s)	970, 1105, 2284
FaeI	CATG	8 cut(s)	616, 742, 759, 1109, 1339, 1722, 1991, 2141
FalI	AAGNNNNNCTT	2 cut(s)	1553, 1585
FaqI	GGGAC	2 cut(s)	98, 311
FatI	CATG	8 cut(s)	612, 738, 755, 1105, 1335, 1718, 1987, 2137
FauI	CCCGC	2 cut(s)	23, 1899
FauNDI	CATATG	1 cut(s)	1096
FokI	GGATG	4 cut(s)	128, 831, 887, 1184
FriOI	GRGCYC	4 cut(s)	242, 394, 424, 1397
FspBI	CTAG	6 cut(s)	27, 678, 1398, 2097, 2285, 2357
GlaI	GCGC	4 cut(s)	67, 222, 475, 2272
GsuI	CTGGAG	1 cut(s)	226
HapII	CCGG	4 cut(s)	450, 1477, 1652, 2337
HgaI	GACGC	4 cut(s)	164, 172, 244, 1603
HhaI	GCGC	4 cut(s)	68, 223, 476, 2273
Hin1I	GRCGYC	1 cut(s)	255
Hin1II	CATG	8 cut(s)	616, 742, 759, 1109, 1339, 1722, 1991, 2141
Hin6I	GCGC	4 cut(s)	66, 221, 474, 2271
HinP1I	GCGC	4 cut(s)	66, 221, 474, 2271
HincII	GTYRAC	1 cut(s)	481
HindII	GTYRAC	1 cut(s)	481
HindIII	AAGCTT	1 cut(s)	355
HinfI	GANTC	6 cut(s)	288, 587, 747, 1965, 2293, 2388
HpaI	GTTAAC	1 cut(s)	481
HpaII	CCGG	4 cut(s)	450, 1477, 1652, 2337
HphI	GGTGA	6 cut(s)	337, 719, 1046, 1361, 1533, 2098
Hpy166II	GTNNAC	5 cut(s)	481, 647, 1674, 2016, 2052
Hpy8I	GTNNAC	5 cut(s)	481, 647, 1674, 2016, 2052
Hpy99I	CGWCG	4 cut(s)	61, 188, 1905, 2121
HpyAV	CCTTC	7 cut(s)	45, 91, 126, 211, 1167, 1628, 2373
HpyCH4III	ACNGT	7 cut(s)	606, 695, 1005, 1214, 1740, 1791, 2056
HpyCH4IV	ACGT	3 cut(s)	786, 926, 1903
HpyF10VI	GCNNNNNNNGC	8 cut(s)	40, 134, 246, 252, 419, 644, 1508, 2450
HpyF3I	CTNAG	7 cut(s)	90, 489, 513, 731, 1918, 2312, 2454
HpySE526I	ACGT	3 cut(s)	786, 926, 1903
Hsp92I	GRCGYC	1 cut(s)	255
Hsp92II	CATG	8 cut(s)	616, 742, 759, 1109, 1339, 1722, 1991, 2141
HspAI	GCGC	4 cut(s)	66, 221, 474, 2271
KspAI	GTTAAC	1 cut(s)	481
LguI	GCTCTTC	1 cut(s)	1232
LweI	GCATC	2 cut(s)	314, 1140
MaeI	CTAG	6 cut(s)	27, 678, 1398, 2097, 2285, 2357
MaeII	ACGT	3 cut(s)	786, 926, 1903
MaeIII	GTNAC	5 cut(s)	695, 1135, 1370, 1953, 2181
MbiI	CCGCTC	1 cut(s)	122
MflI	RGATCY	4 cut(s)	13, 232, 1576, 2494
MhlI	GDGCHC	6 cut(s)	242, 394, 424, 649, 1397, 2194
MlsI	TGGCCA	1 cut(s)	155
MluCI	AATT	9 cut(s)	316, 348, 555, 935, 1218, 1704, 1861, 1997, 2141
MluI	ACGCGT	1 cut(s)	1367
MluNI	TGGCCA	1 cut(s)	155
MlyI	GAGTC	1 cut(s)	282
MmeI	TCCRAC	3 cut(s)	307, 1886, 1924
Mox20I	TGGCCA	1 cut(s)	155
Mph1103I	ATGCAT	2 cut(s)	307, 1989
MscI	TGGCCA	1 cut(s)	155
MseI	TTAA	6 cut(s)	351, 480, 630, 864, 912, 2010
MslI	CAYNNNNRTG	2 cut(s)	92, 1679
Msp20I	TGGCCA	1 cut(s)	155
MspA1I	CMGCKG	2 cut(s)	246, 1894
MspI	CCGG	4 cut(s)	450, 1477, 1652, 2337
MspR9I	CCNGG	8 cut(s)	11, 21, 401, 451, 752, 1652, 1653, 2337
Mva1269I	GAATGC	5 cut(s)	1750, 1768, 1989, 2273, 2342
MvaI	CCWGG	4 cut(s)	11, 21, 401, 752
MvnI	CGCG	3 cut(s)	183, 1369, 1614
MwoI	GCNNNNNNNGC	8 cut(s)	40, 134, 246, 252, 419, 644, 1508, 2450
NciI	CCSGG	4 cut(s)	451, 1652, 1653, 2337
NcoI	CCATGG	1 cut(s)	1105
NdeI	CATATG	1 cut(s)	1096
NlaIII	CATG	8 cut(s)	616, 742, 759, 1109, 1339, 1722, 1991, 2141
NlaIV	GGNNCC	7 cut(s)	15, 234, 455, 794, 1940, 2193, 2324
NmuCI	GTSAC	4 cut(s)	695, 1370, 1953, 2181
NsiI	ATGCAT	2 cut(s)	307, 1989
NspI	RCATGY	1 cut(s)	759
NspV	TTCGAA	2 cut(s)	1465, 2296
OliI	CACNNNNGTG	2 cut(s)	92, 1679
PaeR7I	CTCGAG	1 cut(s)	290
PciSI	GCTCTTC	1 cut(s)	1232
PcsI	WCGNNNNNNNCGW	1 cut(s)	53
PctI	GAATGC	5 cut(s)	1750, 1768, 1989, 2273, 2342
PfeI	GAWTC	5 cut(s)	587, 747, 1965, 2293, 2388
PflFI	GACNNNGTC	1 cut(s)	785
PinAI	ACCGGT	1 cut(s)	1476
Ple19I	CGATCG	1 cut(s)	2281
PleI	GAGTC	1 cut(s)	282
PpsI	GAGTC	1 cut(s)	282
PpuMI	RGGWCCY	1 cut(s)	454
PsiI	TTATAA	2 cut(s)	375, 1199
Psp124BI	GAGCTC	2 cut(s)	242, 394
Psp5II	RGGWCCY	1 cut(s)	454
Psp6I	CCWGG	4 cut(s)	9, 19, 399, 750
PspGI	CCWGG	4 cut(s)	9, 19, 399, 750
PspN4I	GGNNCC	7 cut(s)	15, 234, 455, 794, 1940, 2193, 2324
PspPI	GGNCC	6 cut(s)	23, 32, 454, 735, 1332, 1485
PspPPI	RGGWCCY	1 cut(s)	454
PstI	CTGCAG	1 cut(s)	640
PstNI	CAGNNNCTG	1 cut(s)	43
PsuI	RGATCY	4 cut(s)	13, 232, 1576, 2494
PsyI	GACNNNGTC	1 cut(s)	785
PvuI	CGATCG	1 cut(s)	2281
RsaI	GTAC	3 cut(s)	540, 1211, 2015
RsaNI	GTAC	3 cut(s)	539, 1210, 2014
RseI	CAYNNNNRTG	2 cut(s)	92, 1679
SacI	GAGCTC	2 cut(s)	242, 394
SapI	GCTCTTC	1 cut(s)	1232
SaqAI	TTAA	6 cut(s)	351, 480, 630, 864, 912, 2010
Sau96I	GGNCC	6 cut(s)	23, 32, 454, 735, 1332, 1485
SchI	GAGTC	1 cut(s)	282
ScrFI	CCNGG	8 cut(s)	11, 21, 401, 451, 752, 1652, 1653, 2337
SduI	GDGCHC	6 cut(s)	242, 394, 424, 649, 1397, 2194
SfaNI	GCATC	2 cut(s)	314, 1140
SfcI	CTRYAG	2 cut(s)	636, 2476
Sfr274I	CTCGAG	1 cut(s)	290
SfuI	TTCGAA	2 cut(s)	1465, 2296
SinI	GGWCC	2 cut(s)	454, 1485
SlaI	CTCGAG	1 cut(s)	290
SmaI	CCCGGG	1 cut(s)	1653
SmiMI	CAYNNNNRTG	2 cut(s)	92, 1679
SmlI	CTYRAG	3 cut(s)	290, 1570, 1697
SmoI	CTYRAG	3 cut(s)	290, 1570, 1697
Sse9I	AATT	9 cut(s)	316, 348, 555, 935, 1218, 1704, 1861, 1997, 2141
SspI	AATATT	3 cut(s)	371, 1298, 1693
SspMI	CTAG	6 cut(s)	27, 678, 1398, 2097, 2285, 2357
SstI	GAGCTC	2 cut(s)	242, 394
StyD4I	CCNGG	8 cut(s)	9, 19, 399, 449, 750, 1650, 1651, 2335
StyI	CCWWGG	3 cut(s)	970, 1105, 2284
TaaI	ACNGT	7 cut(s)	606, 695, 1005, 1214, 1740, 1791, 2056
TaiI	ACGT	3 cut(s)	789, 929, 1906
TaqII	GACCGA	1 cut(s)	185
TasI	AATT	9 cut(s)	316, 348, 555, 935, 1218, 1704, 1861, 1997, 2141
TatI	WGTACW	2 cut(s)	538, 2013
TauI	GCSGC	3 cut(s)	37, 249, 527
TfiI	GAWTC	5 cut(s)	587, 747, 1965, 2293, 2388
Tru1I	TTAA	6 cut(s)	351, 480, 630, 864, 912, 2010
Tru9I	TTAA	6 cut(s)	351, 480, 630, 864, 912, 2010
TscAI	CASTG	6 cut(s)	94, 451, 1681, 1743, 2138, 2307
TseFI	GTSAC	4 cut(s)	695, 1370, 1953, 2181
Tsp45I	GTSAC	4 cut(s)	695, 1370, 1953, 2181
TspGWI	ACGGA	2 cut(s)	1204, 2474
TspMI	CCCGGG	1 cut(s)	1651
TspRI	CASTG	6 cut(s)	94, 451, 1681, 1743, 2138, 2307
Tth111I	GACNNNGTC	1 cut(s)	785
VneI	GTGCAC	1 cut(s)	645
VpaK11BI	GGWCC	2 cut(s)	454, 1485
XapI	RAATTY	5 cut(s)	316, 555, 935, 1861, 2141
XbaI	TCTAGA	1 cut(s)	1397
XceI	RCATGY	1 cut(s)	759
XcmI	CCANNNNNNNNNTGG	1 cut(s)	148
XhoI	CTCGAG	1 cut(s)	290
XmaI	CCCGGG	1 cut(s)	1651
XmaJI	CCTAGG	1 cut(s)	2284
XspI	CTAG	6 cut(s)	27, 678, 1398, 2097, 2285, 2357
Zsp2I	ATGCAT	2 cut(s)	307, 1989

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.