Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0007588

Physical Location & Seq

Genomic Coordinates Forward (+)

56391548 .. 56406427

Gene Structure 14880 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00122950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5154 bp

ATGCAGCTTGATGAGGTTAAGGCCGCTGTGGCTGCACTGTGGAATACTCGTGGTTTGAACTGGCCAAGTGCATTCGAGCAGCACAGACAAAAAGCTAATGATCTGGATCTCCTTGATTGGCTTAGGGCCATGTTTGGGTTCCAGAGAGATAACGTCAGGAACCAGAGGGAGCATTTGATATTGCTACTTGCGAATACGCATATAAGACTTCATCCCAAACCTGAACCTCTTAACAAGCTTGATGATCGAGCTGTTGATGCAGTTCTTGACAAGCTTTTTAAGAACTACAAAACGTGGTGTAAATTTTTGGGTCGAAAACATAGTTTAAGGCTACCTCAAGGTCAGCAAGAAGTACAACAGAGGAAGATACTTTACATGGGTTTATATCTTCTCATATGGGGTGAAGCAGCGAATGTTCGCTTTATGCCTGAATGCCTCTGCTATATTTTCCATAATATGGCATATGAACTCCATGGTCTCTTGGCTGGAAATGTCAGCATTGTCACTGGGGAAAATATAAAGCCTTCTTATGGTGGGGATGATGAGGCCTTTTTGAGGAAGGTTATAACCCCCCTATACCGTGTAATCGAAAAGGAAGCCAAGAAGGGAGCCGGTGGAAAAGCTCCTCACACAGCTTGGTGCAACTATGATGATCTAAATGAATATTTCTGGTCATCTGATTGTTTCTCTCTTGGTTGGCCCATGCGTGATGATGGGGATTTTTTTAAATCAACTCGTGACTTGGCACAGGGAAGAAAGGGCTCTAAAAGAAAATCTGGAAGCACAGGCAAATCATATTTTATTGAAACTCGCACATTTTGGCACATCTTTCGAAGTTTTGATCGAATGTGGACCTTTTATATACTGGCTCTACAGGCTATGCTCATTATTGCATTCAGCAAGGTTTCACCGCTGGATATATTTCAACAGGATGTCCTAAAAGATCTATCAAGTATTTTCATTACAGCAGCATTTCTTCGCGTCCTTCAGAGTATTCTGGATATGGTTTTGAACTTCCCTGGATATCACAGGTGTCGGTTCACAGATGTCCTGAGAAATATTCTGAAGATAGTTGTTAGTCTTGCATGGGCAGTCATTCTTCCACTTTTCTATGTGCATTCCTTCAATGATTCCCCTCAACAAATTGCAGATATACTGTCATTCCTTAAGAACATTCAAGGCGTTCCATCATTATATCTATTGGCTGTTGCAGTATATTTGCTTCCAAATTTACTGACAGCAGTTTTGTTCCTTTTCCCAATGCTCCGTCGTTTTATTGAAAATTCAGACTGGCATATCATCAGGTTCCTCTTGTGGTGGTCACAGCCAAGAATATATGTTGGAAGAGGAATGCATGAAAGTCAATTTGCCCTTATAAAGTATACTTTTTTCTGGTTGCTACTTTTGGCTTGCAAGTTTACAGTTAGCTATTTTGTCCAGATAAAACCTCTGGTAGAGCCAACAGAAGACATAATGAAGATTCGTCGTATTAATTATGAGTGGCATGAATTTTTTCCTAATGCTGAACATAACTATGGAGCAGTTGTCTCACTCTGGGCACCTGTTATCATGGTTTATTTCATGGACACACAAATTTGGTATGCTATATTTCAAACATTGTATGGAGGTATTGTTGGTGCCTTTGATCGCCTAGGAGAGATTCGTACTTTAGGCATGCTAAGATCCCGGTTCCAGTCTCTACCTGGTGCATTCAACACATATCTAGTGCCCTCGGATAAGTCAACAAAAAGGGGATTCTCTTTCTCAAAGAAATTTGCGGAGGCTTCAGCAAGTAGGAGAAGTGAAGCTGCAAAGTTCGCTCAGTTGTGGAATGAAGTGATATGTAGCTTTCGTGAGGAAGATCTCATCAGCGACAGGGAAATGGATTTGCTGCTGGTTCCTTATTCTTCAGATACGAGCCTGAAAATAATTCAGTGGCCACCTTTCTTGCTTGCTAGCAAAATCCCAATAGCATTAGATATGGCAGCTCAATTCAAAAACAAGGACTCTGATCTCTGGAAGCGGATATGTGGAGATGAATATATGAAGTGTGCTGTGATTGAATGTTATGAGTCTTTCAAACATGTCCTTAAGGCTTTGGTGGTTGGAGAAAATGAGAAAAGGATCATTGGCATCATTATTAAGGAAATTGAAAGTAACATTTCCAAGAATACATTTCTTGATAATTTTAGAATGGCCTCTTTACCCACTCTCTGCCAGAAATTTGTAGAGCTTGTGGTGATCTTGAAAGATGGTGATTCATCCAAGCGATCTGCGGTGGTGCTGCTGCTGCAAGATATGTTGGAAGTTGTCACTCGTGATATGATGGTGAATGAGATCCGAGAGGTGGTTGAAGTTGGTCATAGTAGCAAGGACACTGGAAGGCAACTTTTTGCTGGTACTGATGCAAAACCTGCGATAGTGTTCCCTCCTCCAGTTACAGCACAATGGGAAGAACAGATAAGGCGCCTCCATCTGCTGTTGACCGTTAAGGAGTCTGCAATTGATGTACCAATGAACCTTGAAGCACGTAGAAGGATTTCATTCTTTGCAAATTCATTGTTCATGGATATGCCCCGTGCTCCCCGTGTTCGTAAGATGCTTTCGTTCAGCATTTTGACTCCCTACTATAGTGAGGAGACTGTGTATTCCAAAAGTGACCTTGAGATGGAAAATGAAGATGGTGTATCAATCATATATTACTTGCAGAAGATTTTCCCAGATGAATGGACTAACTTCATGGAGAGGCTTAATTGTAAAAAGGATGCTGAAATATGGGAAAATGAAGAGAACATTTTGCAGCTGCGTCATTGGGTCTCCTTACGAGGACAAACTCTCTGCAGGACAGTTAGAGGAATGATGTATTACCGACGAGCTCTGAAGCTTCAGGCTTTTCTTGATATGGCTAATGAAGAAGAGATACTAGAAGGGTACAAAGCCGTCACAGTTTCACCAGAGGACGAAAAGAAGAGCCGGAGATCCCTATTTGCTCAACTAGAAGCAGTGGCTGATCTTAAATTTACCTATGTTGCTACTTGCCAAAACTATGGCAATCAGAAGCGAAGTGGAGATCGTCGTGCAACTGACATCCTGAATCTGATGGTTAATAATCCCTCTCTCAGGGTTGCATATATTGATGAAGTTGAAGAACGAGAAGGTGAAAAGGTGCAGAAGGTTTACTATTCTGTATTGGTCAAAGCAGCTGACAACCTTGACCAGGAAATATATCGTATAAAATTGCCGGGCTCAGCAAAGATTGGAGAAGGGAAGCCTGAAAACCAGAACCATGCAGTAATCTTTACTCGTGGAGAAGCTCTTCAGGCTATTGACATGAATCAGGATAATTACTTGGAAGAGGCTCTTAAGATGCGTAACCTTCTTGAAGAATTTAATGAGGATCACGGAGTGCGTTCACCTTCAATTTTAGGTGTTCGTGAGCATATCTTTACTGGAAGTGTTTCTTCGTTGGCGTGGTTTATGTCAAATCAAGAAATGAGCTTCGTGACCATTGGTCAAAGAGTTCTTGCTAGACCTCTTAAGGTTCGGTTCCATTATGGACATCCGGATGTCTTTGATAGAATCTTCCATATTACTCGTGGTGGCATGAGCAAGGCTTCTCGTGGGATCAATCTCAGCGAGGATATTTTTGCAGGTTTCAATTCCACTCTAAGAAGAGGAAATGTCACTCACCATGAGTATATCCAAGTTGGGAAGGGTAGGGATGTTGGCCTCAATCAAATCTCTCTCTTTGAGGCTAAAGTTGCTTGCGGTAATGGAGAGCAGACACTTAGCAGAGACATCTATCGATTAGGGCACCGTTATGATTTCTTCCGCATGTTGTCATATTACTTTTCAACAATAGGATTTTATATCAGCTCAATGTTGGTTGTGATGACGGTCTACTCTTTCCTGTATGGTAGACTCTACTTGTCATTGAGTGGAATGGAGAAGACAATTATGAACTATGCTGCCAGAAGGGGAAATAATGCTCTACAAACAGCTATGGCTTCACAATCTGTTGTCCAATTAGGTTTGTTGACGTCCTTGCCCATGATCATGGAAATCGGACTTGAAAGAGGGTTCAGAACTGCATTAGGGGACACGATAATAATGCAGCTGCAGCTAGCATCAGTTTTCTTTACTTTCTCACTGGGTACAAAGGTTCATTACTATGGGAGAACAATCCTGCATGGTGGGGCTAAGTACAGAGCAACTGGTCGTGGCTTTGTTGTTCGGCATGAAAGGTTTGGTGAAAACTACAGGATGTACTCACGGAGTCACTTTACAAAAGGCCTGGAGCTTATGATACTGCTTATTATTTATAGTATATATGGCTCAGCAGCAGATGGCTCAGCTGCATATCTCTTCATCACATTCTCCATGTGGTTCTTAGTGATTTCTTGGTTATTTGCCCCCTTCCTCTTCAACCCTTCGGGATTTGAATGGCAAAAAATAGTGGAAGATTGGGATGATTGGTCAAAATGGATAACTAGCCGTGGTGGTATTGGTGTGCCAGCAAATAAGAGCTGGGAGTCCTGGTGGGATGAGGAACAGGAGCACCTGCAGCACACTGGGATGTTGGGACGGTTTTGGGAGATTGTACTTTCTCTGCGCTTCTTTCTTTTCCAATATGGAATTGTCTACCATCTCAATGTTTCCAGGGGTGATAAAAGTATCATGGTTTATGCTCTGTCATGGCTCGTCATTGTTGCTGTGATGATTATCCTCAAGATTGTATCCTTGGGTAGAAAAAGGTTCAGTGCTGATTTCCAGCTTATGTTCAGACTTCTGAAGTTGTTCGTCTTCATTGGGTTTGTCGTCACCATTGCAGTACTTTTTATTTTCCTTAGTCTTACTGTTGGTGACATCTTTGTAAGCTTACTGGCCTTCTTGCCTACTGGGTGGGCAATACTTATGATATCACAAGCATGCAGGCCATTGGTGAAGGTCTTAGGAATGTGGGGGTCTGTAAAAGCTCTAGCAAGAGGGTATGAGTACGTGATGGGACTGGTTATCTTTACCCCAGTGGCTGTTCTGGCATGGTTTCCATTTGTCTCAGAGTTCCAGACCAGGCTGCTATTCAACCAGGCTTTCAGTCGAGGCCTTCAGATCCAACGTATTCTTTCTGGTGGGAAGAAGCAAAAGTCCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006075 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009555 GO:0009556 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010208 GO:0010769 GO:0010927 GO:0016043 GO:0016049 GO:0016051 GO:0019953 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034293 GO:0034637 GO:0034645 GO:0040007 GO:0040008 GO:0043062 GO:0043170 GO:0043900 GO:0043934 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048236 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048646 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051273 GO:0051274 GO:0051321 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0080092 GO:0085029 GO:1901576 GO:1903046 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1717

Amino Acids

198.04

Weight (kDa)

9.06

Isoelectric Point (pI)

41.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FKS1_dom1	PF14288	122 - 233	3.7e-41	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	647 - 830	8.4e-68	Callose synthase 1 domain
Glucan_synthase	PF02364	837 - 924	3.8e-27	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	928 - 1531	7.6e-207	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13680
fragaria_vesca	FvH4_1g24300
malus_domestica	MD01G1004000.v1.1 MD01G1004100.v1.1 MD02G1211500.v1.1
prunus_persica	Prupe.4G271000_v2.0.a1
pyrus_communis	pycom01g02040 pycom15g30380
rosa_chinensis	RchiOBHm_Chr2g0120781
rosa_laevigata	RLG00000018581 RLG00000018588
rosa_roxburghii	Rroxscaffold_2G00122840 Rroxscaffold_2G00122950
rosa_rugosa	Rorug02G0231300
rosa_samantha	Rh2AG287900 Rh2AG288000 Rh2AG288100 Rh2BG299200 Rh2BG299300 Rh2CG277200 Rh2CG277300 Rh2DG313100 Rh2DG313200
rosa_wichuraiana	Rw2G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	566, 1376
AarI	CACCTGC	1 cut(s)	4569
AatII	GACGTC	1 cut(s)	4043
Acc36I	ACCTGC	3 cut(s)	2422, 3641, 4569
AccB1I	GGYRCC	4 cut(s)	1558, 1637, 2466, 3813
AccB7I	CCANNNNNTGG	1 cut(s)	457
AccI	GTMKAC	4 cut(s)	1382, 3900, 3919, 4641
AccII	CGCG	1 cut(s)	981
AccIII	TCCGGA	1 cut(s)	3562
AciI	CCGC	7 cut(s)	24, 911, 1778, 2023, 2276, 3768, 3832
AclWI	GGATC	8 cut(s)	114, 1677, 2132, 2332, 2973, 3405, 3632, 5104
AcoI	YGGCCR	2 cut(s)	62, 1937
AcuI	CTGAAG	9 cut(s)	971, 1085, 1770, 1893, 2870, 2900, 3302, 4811, 5090
AcyI	GRCGYC	2 cut(s)	2467, 4040
AdeI	CACNNNGTG	1 cut(s)	3406
AfiI	CCNNNNNNNGG	8 cut(s)	135, 457, 530, 555, 2347, 2668, 3120, 3217
AflII	CTTAAG	4 cut(s)	1166, 2090, 3362, 3536
AflIII	ACRYGT	1 cut(s)	2083
AhdI	GACNNNNNGTC	1 cut(s)	3510
AjnI	CCWGG	8 cut(s)	1018, 1702, 3216, 4293, 4535, 4658, 5069, 5085
Alw21I	GWGCWC	3 cut(s)	2584, 2878, 4560
Alw26I	GTCTC	7 cut(s)	482, 1552, 1701, 2633, 2821, 3789, 5059
AlwI	GGATC	8 cut(s)	114, 1677, 2132, 2332, 2973, 3405, 3632, 5104
AlwNI	CAGNNNCTG	2 cut(s)	3008, 5030
Aor13HI	TCCGGA	1 cut(s)	3562
ArsI	GACNNNNNNTTYG	2 cut(s)	1228, 1260
AseI	ATTAAT	1 cut(s)	1491
Asp700I	GAANNNNTTC	6 cut(s)	1512, 2539, 2714, 3315, 3457, 4796
AspA2I	CCTAGG	1 cut(s)	1651
AspLEI	GCGC	2 cut(s)	2469, 4614
AspS9I	GGNCC	3 cut(s)	126, 699, 852
AsuC2I	CCSGG	2 cut(s)	1687, 3243
AsuII	TTCGAA	1 cut(s)	832
AsuNHI	GCTAGC	2 cut(s)	1955, 4123
AvaII	GGWCC	1 cut(s)	852
AvrII	CCTAGG	1 cut(s)	1651
BaeGI	GKGCMC	3 cut(s)	1561, 1731, 3816
BaeI	ACNNNNGTAYC	2 cut(s)	4146, 4179
BalI	TGGCCA	2 cut(s)	64, 1939
BanI	GGYRCC	4 cut(s)	1558, 1637, 2466, 3813
BanII	GRGCYC	3 cut(s)	764, 2878, 3248
BarI	GAAGNNNNNNTAC	6 cut(s)	356, 388, 1206, 1238, 2904, 2936
BauI	CACGAG	6 cut(s)	48, 735, 2316, 3303, 3594, 3618
BbsI	GAAGAC	3 cut(s)	1473, 3956, 4795
Bbv12I	GWGCWC	3 cut(s)	2584, 2878, 4560
BceAI	ACGGC	2 cut(s)	2924, 4479
BcgI	CGANNNNNNTGC	4 cut(s)	812, 846, 4093, 4127
BciT130I	CCWGG	8 cut(s)	1020, 1704, 3218, 4295, 4537, 4660, 5071, 5087
BciVI	GTATCC	1 cut(s)	4747
BclI	TGATCA	1 cut(s)	4053
BcnI	CCSGG	2 cut(s)	1687, 3243
BcoDI	GTCTC	7 cut(s)	482, 1552, 1701, 2633, 2821, 3789, 5059
BfaI	CTAG	9 cut(s)	1652, 1724, 1956, 2924, 2996, 3528, 4124, 4491, 4979
BfmI	CTRYAG	6 cut(s)	872, 2629, 2839, 4118, 4258, 4562
BfoI	RGCGCY	1 cut(s)	2470
BfrI	CTTAAG	4 cut(s)	1166, 2090, 3362, 3536
BfuAI	ACCTGC	3 cut(s)	2422, 3641, 4569
BfuI	GTATCC	1 cut(s)	4747
BglI	GCCNNNNNGGC	1 cut(s)	29
BglII	AGATCT	2 cut(s)	943, 1861
BlnI	CCTAGG	1 cut(s)	1651
BlpI	GCTNAGC	3 cut(s)	3247, 4336, 4351
BmcAI	AGTACT	1 cut(s)	4833
Bme18I	GGWCC	1 cut(s)	852
BmeRI	GACNNNNNGTC	1 cut(s)	3510
BmgT120I	GGNCC	3 cut(s)	126, 699, 852
BmrI	ACTGGG	5 cut(s)	516, 4160, 4581, 4908, 5018
BmsI	GCATC	7 cut(s)	247, 2142, 2395, 2589, 2755, 3357, 4136
BmtI	GCTAGC	2 cut(s)	1959, 4127
BmuI	ACTGGG	5 cut(s)	516, 4160, 4581, 4908, 5018
BpiI	GAAGAC	3 cut(s)	1473, 3956, 4795
BpmI	CTGGAG	2 cut(s)	2418, 4316
Bpu10I	CCTNAGC	1 cut(s)	122
Bpu1102I	GCTNAGC	3 cut(s)	3247, 4336, 4351
Bpu14I	TTCGAA	1 cut(s)	832
BpuEI	CTTGAG	3 cut(s)	321, 2684, 4712
BpuMI	CCSGG	2 cut(s)	1687, 3243
Bsa29I	ATCGAT	1 cut(s)	3805
BsaAI	YACGTR	2 cut(s)	2531, 4999
BsaBI	GATNNNNATC	2 cut(s)	105, 4721
BsaHI	GRCGYC	2 cut(s)	2467, 4040
BsaI	GGTCTC	2 cut(s)	482, 2821
BsaJI	CCNNGG	7 cut(s)	472, 1018, 1651, 1731, 4495, 4659, 4740
BsaWI	WCCGGW	1 cut(s)	3562
BsaXI	ACNNNNNCTCC	8 cut(s)	1530, 1560, 1617, 1647, 2630, 2660, 4523, 4553
Bsc4I	CCNNNNNNNGG	8 cut(s)	135, 457, 530, 555, 2347, 2668, 3120, 3217
Bse118I	RCCGGY	1 cut(s)	611
Bse3DI	GCAATG	1 cut(s)	4824
Bse8I	GATNNNNATC	2 cut(s)	105, 4721
BseAI	TCCGGA	1 cut(s)	3562
BseBI	CCWGG	8 cut(s)	1020, 1704, 3218, 4295, 4537, 4660, 5071, 5087
BseCI	ATCGAT	1 cut(s)	3805
BseDI	CCNNGG	7 cut(s)	472, 1018, 1651, 1731, 4495, 4659, 4740
BseJI	GATNNNNATC	2 cut(s)	105, 4721
BseLI	CCNNNNNNNGG	8 cut(s)	135, 457, 530, 555, 2347, 2668, 3120, 3217
BseMI	GCAATG	1 cut(s)	4824
BseMII	CTCAG	8 cut(s)	1043, 1835, 3133, 3261, 3646, 4350, 4365, 5070
BseRI	GAGGAG	3 cut(s)	615, 2421, 2651
BseSI	GKGCMC	3 cut(s)	1561, 1731, 3816
BseYI	CCCAGC	1 cut(s)	4527
BsgI	GTGCAG	2 cut(s)	18, 3188
Bsh1236I	CGCG	1 cut(s)	981
BshNI	GGYRCC	4 cut(s)	1558, 1637, 2466, 3813
BshVI	ATCGAT	1 cut(s)	3805
BsiHKAI	GWGCWC	3 cut(s)	2584, 2878, 4560
BsiSI	CCGG	5 cut(s)	612, 1687, 2974, 3242, 3563
BslFI	GGGAC	3 cut(s)	4112, 4596, 5019
BslI	CCNNNNNNNGG	8 cut(s)	135, 457, 530, 555, 2347, 2668, 3120, 3217
BsmAI	GTCTC	7 cut(s)	482, 1552, 1701, 2633, 2821, 3789, 5059
BsmFI	GGGAC	3 cut(s)	4112, 4596, 5019
BsmI	GAATGC	6 cut(s)	71, 437, 893, 1117, 1356, 1709
Bso31I	GGTCTC	2 cut(s)	482, 2821
Bsp119I	TTCGAA	1 cut(s)	832
Bsp1286I	GDGCHC	8 cut(s)	764, 1561, 1731, 2584, 2878, 3248, 3816, 4560
Bsp13I	TCCGGA	1 cut(s)	3562
Bsp1720I	GCTNAGC	3 cut(s)	3247, 4336, 4351
Bsp19I	CCATGG	1 cut(s)	472
BspACI	CCGC	7 cut(s)	24, 911, 1778, 2023, 2276, 3768, 3832
BspCNI	CTCAG	8 cut(s)	1044, 1834, 3132, 3260, 3645, 4349, 4364, 5069
BspDI	ATCGAT	1 cut(s)	3805
BspEI	TCCGGA	1 cut(s)	3562
BspFNI	CGCG	1 cut(s)	981
BspMAI	CTGCAG	3 cut(s)	2843, 4122, 4566
BspMI	ACCTGC	3 cut(s)	2422, 3641, 4569
BspOI	GCTAGC	2 cut(s)	1959, 4127
BspPI	GGATC	8 cut(s)	114, 1677, 2132, 2332, 2973, 3405, 3632, 5104
BspQI	GCTCTTC	2 cut(s)	2963, 3321
BspT104I	TTCGAA	1 cut(s)	832
BspT107I	GGYRCC	4 cut(s)	1558, 1637, 2466, 3813
BspTI	CTTAAG	4 cut(s)	1166, 2090, 3362, 3536
BspTNI	GGTCTC	2 cut(s)	482, 2821
BsrDI	GCAATG	1 cut(s)	4824
BsrFI	RCCGGY	1 cut(s)	611
BssAI	RCCGGY	1 cut(s)	611
BssECI	CCNNGG	7 cut(s)	472, 1018, 1651, 1731, 4495, 4659, 4740
BssNAI	GTATAC	1 cut(s)	1383
BssNI	GRCGYC	2 cut(s)	2467, 4040
BssSI	CACGAG	6 cut(s)	48, 735, 2316, 3303, 3594, 3618
BssT1I	CCWWGG	3 cut(s)	472, 1651, 4740
Bst1107I	GTATAC	1 cut(s)	1383
Bst2BI	CACGAG	6 cut(s)	48, 735, 2316, 3303, 3594, 3618
Bst2UI	CCWGG	8 cut(s)	1020, 1704, 3218, 4295, 4537, 4660, 5071, 5087
Bst6I	CTCTTC	9 cut(s)	1339, 2781, 2910, 2963, 3321, 3348, 3667, 4370, 4427
BstACI	GRCGYC	2 cut(s)	2467, 4040
BstAFI	CTTAAG	4 cut(s)	1166, 2090, 3362, 3536
BstAPI	GCANNNNNTGC	1 cut(s)	2414
BstBAI	YACGTR	2 cut(s)	2531, 4999
BstBI	TTCGAA	1 cut(s)	832
BstC8I	GCNNGC	9 cut(s)	1411, 1676, 1953, 1957, 3766, 4125, 4515, 4930, 4934
BstDSI	CCRYGG	2 cut(s)	472, 4495
BstENI	CCTNNNNNAGG	3 cut(s)	553, 3118, 3215
BstFNI	CGCG	1 cut(s)	981
BstH2I	RGCGCY	1 cut(s)	2470
BstHHI	GCGC	2 cut(s)	2469, 4614
BstMAI	GTCTC	7 cut(s)	482, 1552, 1701, 2633, 2821, 3789, 5059
BstNI	CCWGG	8 cut(s)	1020, 1704, 3218, 4295, 4537, 4660, 5071, 5087
BstNSI	RCATGY	4 cut(s)	1678, 2087, 3838, 4932
BstSFI	CTRYAG	6 cut(s)	872, 2629, 2839, 4118, 4258, 4562
BstSLI	GKGCMC	3 cut(s)	1561, 1731, 3816
BstUI	CGCG	1 cut(s)	981
BstV2I	GAAGAC	3 cut(s)	1473, 3956, 4795
BstX2I	RGATCY	7 cut(s)	106, 943, 1682, 1861, 2337, 2978, 5109
BstXI	CCANNNNNNTGG	3 cut(s)	2727, 3047, 4057
BstYI	RGATCY	7 cut(s)	106, 943, 1682, 1861, 2337, 2978, 5109
BstZ17I	GTATAC	1 cut(s)	1383
Bsu15I	ATCGAT	1 cut(s)	3805
BsuI	GTATCC	1 cut(s)	4747
BsuTUI	ATCGAT	1 cut(s)	3805
BtgI	CCRYGG	2 cut(s)	472, 4495
BtsI	GCAGTG	1 cut(s)	3009
BtsIMutI	CAGTG	9 cut(s)	35, 504, 1940, 2376, 3009, 4148, 4569, 4765, 5031
BveI	ACCTGC	3 cut(s)	2422, 3641, 4569
Cac8I	GCNNGC	9 cut(s)	1411, 1676, 1953, 1957, 3766, 4125, 4515, 4930, 4934
CaiI	CAGNNNCTG	2 cut(s)	3008, 5030
CfoI	GCGC	2 cut(s)	2469, 4614
Cfr10I	RCCGGY	1 cut(s)	611
Cfr13I	GGNCC	3 cut(s)	126, 699, 852
ClaI	ATCGAT	1 cut(s)	3805
CseI	GACGC	2 cut(s)	970, 2795
CsiI	ACCWGGT	1 cut(s)	1702
CspCI	CAANNNNNGTGG	2 cut(s)	1929, 1964
DinI	GGCGCC	1 cut(s)	2468
DraI	TTTAAA	1 cut(s)	727
DraIII	CACNNNGTG	1 cut(s)	3406
DriI	GACNNNNNGTC	1 cut(s)	3510
EaeI	YGGCCR	2 cut(s)	62, 1937
Eam1104I	CTCTTC	9 cut(s)	1339, 2781, 2910, 2963, 3321, 3348, 3667, 4370, 4427
Eam1105I	GACNNNNNGTC	1 cut(s)	3510
EarI	CTCTTC	9 cut(s)	1339, 2781, 2910, 2963, 3321, 3348, 3667, 4370, 4427
Ecl136II	GAGCTC	1 cut(s)	2876
Eco130I	CCWWGG	3 cut(s)	472, 1651, 4740
Eco147I	AGGCCT	3 cut(s)	548, 4293, 5103
Eco24I	GRGCYC	3 cut(s)	764, 2878, 3248
Eco31I	GGTCTC	2 cut(s)	482, 2821
Eco32I	GATATC	2 cut(s)	1025, 4920
Eco47I	GGWCC	1 cut(s)	852
Eco53kI	GAGCTC	1 cut(s)	2876
Eco57I	CTGAAG	9 cut(s)	971, 1085, 1770, 1893, 2870, 2900, 3302, 4811, 5090
EcoICRI	GAGCTC	1 cut(s)	2876
EcoNI	CCTNNNNNAGG	3 cut(s)	553, 3118, 3215
EcoRII	CCWGG	8 cut(s)	1018, 1702, 3216, 4293, 4535, 4658, 5069, 5085
EcoRV	GATATC	2 cut(s)	1025, 4920
EcoT14I	CCWWGG	3 cut(s)	472, 1651, 4740
EcoT22I	ATGCAT	1 cut(s)	1356
EcoT38I	GRGCYC	3 cut(s)	764, 2878, 3248
EgeI	GGCGCC	1 cut(s)	2468
EheI	GGCGCC	1 cut(s)	2468
ErhI	CCWWGG	3 cut(s)	472, 1651, 4740
FalI	AAGNNNNNCTT	4 cut(s)	3345, 3377, 3445, 3477
FaqI	GGGAC	3 cut(s)	4112, 4596, 5019
FauNDI	CATATG	2 cut(s)	395, 463
FbaI	TGATCA	1 cut(s)	4053
FblI	GTMKAC	4 cut(s)	1382, 3900, 3919, 4641
FriOI	GRGCYC	3 cut(s)	764, 2878, 3248
FspBI	CTAG	9 cut(s)	1652, 1724, 1956, 2924, 2996, 3528, 4124, 4491, 4979
GlaI	GCGC	2 cut(s)	2468, 4613
GsaI	CCCAGC	1 cut(s)	4531
GsuI	CTGGAG	2 cut(s)	2418, 4316
HaeII	RGCGCY	1 cut(s)	2470
HapII	CCGG	5 cut(s)	612, 1687, 2974, 3242, 3563
HgaI	GACGC	2 cut(s)	970, 2795
HhaI	GCGC	2 cut(s)	2469, 4614
Hin1I	GRCGYC	2 cut(s)	2467, 4040
Hin6I	GCGC	2 cut(s)	2467, 4612
HinP1I	GCGC	2 cut(s)	2467, 4612
HincII	GTYRAC	3 cut(s)	1743, 2484, 4038
HindII	GTYRAC	3 cut(s)	1743, 2484, 4038
HindIII	AAGCTT	4 cut(s)	236, 272, 2882, 4876
HpaII	CCGG	5 cut(s)	612, 1687, 2974, 3242, 3563
Hpy99I	CGWCG	4 cut(s)	1272, 1488, 2874, 3078
HpyCH4IV	ACGT	6 cut(s)	153, 293, 2530, 4040, 4998, 5116
HpySE526I	ACGT	6 cut(s)	153, 293, 2530, 4040, 4998, 5116
Hsp92I	GRCGYC	2 cut(s)	2467, 4040
HspAI	GCGC	2 cut(s)	2467, 4612
KasI	GGCGCC	1 cut(s)	2466
Kpn2I	TCCGGA	1 cut(s)	3562
Ksp22I	TGATCA	1 cut(s)	4053
LguI	GCTCTTC	2 cut(s)	2963, 3321
LmnI	GCTCC	8 cut(s)	169, 608, 628, 1269, 1538, 2587, 4297, 4555
LweI	GCATC	7 cut(s)	247, 2142, 2395, 2589, 2755, 3357, 4136
MabI	ACCWGGT	1 cut(s)	1702
MaeI	CTAG	9 cut(s)	1652, 1724, 1956, 2924, 2996, 3528, 4124, 4491, 4979
MaeII	ACGT	6 cut(s)	153, 293, 2530, 4040, 4998, 5116
MfeI	CAATTG	1 cut(s)	2502
MflI	RGATCY	7 cut(s)	106, 943, 1682, 1861, 2337, 2978, 5109
MhlI	GDGCHC	8 cut(s)	764, 1561, 1731, 2584, 2878, 3248, 3816, 4560
MlsI	TGGCCA	2 cut(s)	64, 1939
MluNI	TGGCCA	2 cut(s)	64, 1939
Mly113I	GGCGCC	1 cut(s)	2467
MlyI	GAGTC	7 cut(s)	2000, 2081, 2504, 2614, 3915, 4285, 4541
MmeI	TCCRAC	4 cut(s)	1321, 2086, 2283, 5137
Mox20I	TGGCCA	2 cut(s)	64, 1939
Mph1103I	ATGCAT	1 cut(s)	1356
MroI	TCCGGA	1 cut(s)	3562
MroXI	GAANNNNTTC	6 cut(s)	1512, 2539, 2714, 3315, 3457, 4796
MscI	TGGCCA	2 cut(s)	64, 1939
MslI	CAYNNNNRTG	9 cut(s)	1533, 2570, 3564, 3819, 4055, 4170, 4574, 4650, 4927
Msp20I	TGGCCA	2 cut(s)	64, 1939
MspA1I	CMGCKG	6 cut(s)	26, 913, 2803, 3203, 4117, 4355
MspCI	CTTAAG	4 cut(s)	1166, 2090, 3362, 3536
MspI	CCGG	5 cut(s)	612, 1687, 2974, 3242, 3563
MunI	CAATTG	1 cut(s)	2502
Mva1269I	GAATGC	6 cut(s)	71, 437, 893, 1117, 1356, 1709
MvaI	CCWGG	8 cut(s)	1020, 1704, 3218, 4295, 4537, 4660, 5071, 5087
MvnI	CGCG	1 cut(s)	981
NarI	GGCGCC	1 cut(s)	2467
NciI	CCSGG	2 cut(s)	1687, 3243
NcoI	CCATGG	1 cut(s)	472
NdeI	CATATG	2 cut(s)	395, 463
NheI	GCTAGC	2 cut(s)	1955, 4123
NsiI	ATGCAT	1 cut(s)	1356
NspI	RCATGY	4 cut(s)	1678, 2087, 3838, 4932
NspV	TTCGAA	1 cut(s)	832
PaeI	GCATGC	2 cut(s)	1678, 4932
PaqCI	CACCTGC	1 cut(s)	4569
PceI	AGGCCT	3 cut(s)	548, 4293, 5103
PciI	ACATGT	1 cut(s)	2083
PciSI	GCTCTTC	2 cut(s)	2963, 3321
PctI	GAATGC	6 cut(s)	71, 437, 893, 1117, 1356, 1709
PdmI	GAANNNNTTC	6 cut(s)	1512, 2539, 2714, 3315, 3457, 4796
PfeI	GAWTC	8 cut(s)	1130, 1480, 1660, 1755, 2258, 3094, 3334, 3579
PflMI	CCANNNNNTGG	1 cut(s)	457
PleI	GAGTC	7 cut(s)	2000, 2080, 2503, 2614, 3915, 4284, 4540
PluTI	GGCGCC	1 cut(s)	2470
PpsI	GAGTC	7 cut(s)	2000, 2080, 2503, 2614, 3915, 4284, 4540
Ppu21I	YACGTR	2 cut(s)	2531, 4999
PscI	ACATGT	1 cut(s)	2083
PshBI	ATTAAT	1 cut(s)	1491
PsiI	TTATAA	2 cut(s)	566, 1376
Psp124BI	GAGCTC	1 cut(s)	2878
Psp6I	CCWGG	8 cut(s)	1018, 1702, 3216, 4293, 4535, 4658, 5069, 5085
PspFI	CCCAGC	1 cut(s)	4527
PspGI	CCWGG	8 cut(s)	1018, 1702, 3216, 4293, 4535, 4658, 5069, 5085
PspPI	GGNCC	3 cut(s)	126, 699, 852
PstI	CTGCAG	3 cut(s)	2843, 4122, 4566
PstNI	CAGNNNCTG	2 cut(s)	3008, 5030
PsuI	RGATCY	7 cut(s)	106, 943, 1682, 1861, 2337, 2978, 5109
PvuII	CAGCTG	4 cut(s)	2803, 3203, 4117, 4355
RseI	CAYNNNNRTG	9 cut(s)	1533, 2570, 3564, 3819, 4055, 4170, 4574, 4650, 4927
SacI	GAGCTC	1 cut(s)	2878
SapI	GCTCTTC	2 cut(s)	2963, 3321
Sau96I	GGNCC	3 cut(s)	126, 699, 852
ScaI	AGTACT	1 cut(s)	4833
SchI	GAGTC	7 cut(s)	2000, 2081, 2504, 2614, 3915, 4285, 4541
SduI	GDGCHC	8 cut(s)	764, 1561, 1731, 2584, 2878, 3248, 3816, 4560
SexAI	ACCWGGT	1 cut(s)	1702
SfaNI	GCATC	7 cut(s)	247, 2142, 2395, 2589, 2755, 3357, 4136
SfcI	CTRYAG	6 cut(s)	872, 2629, 2839, 4118, 4258, 4562
SfoI	GGCGCC	1 cut(s)	2468
SfuI	TTCGAA	1 cut(s)	832
SinI	GGWCC	1 cut(s)	852
SmiMI	CAYNNNNRTG	9 cut(s)	1533, 2570, 3564, 3819, 4055, 4170, 4574, 4650, 4927
SmlI	CTYRAG	7 cut(s)	336, 1166, 2090, 2663, 3362, 3536, 4727
SmoI	CTYRAG	7 cut(s)	336, 1166, 2090, 2663, 3362, 3536, 4727
SphI	GCATGC	2 cut(s)	1678, 4932
SseBI	AGGCCT	3 cut(s)	548, 4293, 5103
SsiI	CCGC	7 cut(s)	24, 911, 1778, 2023, 2276, 3768, 3832
SspDI	GGCGCC	1 cut(s)	2466
SspI	AATATT	2 cut(s)	665, 1060
SspMI	CTAG	9 cut(s)	1652, 1724, 1956, 2924, 2996, 3528, 4124, 4491, 4979
SstI	GAGCTC	1 cut(s)	2878
StuI	AGGCCT	3 cut(s)	548, 4293, 5103
StyI	CCWWGG	3 cut(s)	472, 1651, 4740
TaiI	ACGT	6 cut(s)	156, 296, 2533, 4043, 5001, 5119
TaqI	TCGA	8 cut(s)	75, 247, 313, 588, 832, 844, 3805, 5098
TatI	WGTACW	5 cut(s)	352, 4203, 4266, 4600, 4831
TauI	GCSGC	1 cut(s)	26
TfiI	GAWTC	8 cut(s)	1130, 1480, 1660, 1755, 2258, 3094, 3334, 3579
TscAI	CASTG	9 cut(s)	42, 511, 1940, 2383, 3009, 4155, 4576, 4765, 5031
TspGWI	ACGGA	3 cut(s)	1256, 3417, 4288
TspRI	CASTG	9 cut(s)	42, 511, 1940, 2383, 3009, 4155, 4576, 4765, 5031
Van91I	CCANNNNNTGG	1 cut(s)	457
Vha464I	CTTAAG	4 cut(s)	1166, 2090, 3362, 3536
VpaK11BI	GGWCC	1 cut(s)	852
VspI	ATTAAT	1 cut(s)	1491
XagI	CCTNNNNNAGG	3 cut(s)	553, 3118, 3215
XceI	RCATGY	4 cut(s)	1678, 2087, 3838, 4932
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1700, 3593
XmaJI	CCTAGG	1 cut(s)	1651
XmiI	GTMKAC	4 cut(s)	1382, 3900, 3919, 4641
XmnI	GAANNNNTTC	6 cut(s)	1512, 2539, 2714, 3315, 3457, 4796
XspI	CTAG	9 cut(s)	1652, 1724, 1956, 2924, 2996, 3528, 4124, 4491, 4979
ZraI	GACGTC	1 cut(s)	4041
ZrmI	AGTACT	1 cut(s)	4833
Zsp2I	ATGCAT	1 cut(s)	1356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.