Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0007588

Physical Location & Seq

Genomic Coordinates Reverse (-)

33600698 .. 33615699

Gene Structure 15002 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ49336

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5682 bp

ATGGCGGCTCCAGATTCCGGCCCCCAGGGCCTGACGCGACGGCCGTCACGGAGCGCCGCGACGACGACGTTTTCGACGGAGGTCTTCGACAACGAGGTCGTCCCCTCCGCCATCGCCCCTATCGCTCCGCTCCTCCGCGTCGCCAACGAAGTCGAGCCCGAGCGCCCTCGCGTCGCCTATCTCTGTCGGTTCTACGCGTTTGAGAAGGCGCACAGATTGGACCCCGGCTCCAGCGGCCGTGGTGTGAGGCAGTTCAAGACCTTGCTCCTGCAGCGATTGGAGAGGGAAAATGCATCAAGTCTTTCTGCTCGAGTTAAAAAGTCTGATGCCAGGGAAATTACAAGCTTCTATCAGCAATATTATGATCACTATGTTAAAGCTGTTCAAGAGGAGCAGGTAGACAGAGCTCAAGTGAGTAAAGCCTACAATACACTTAACCAGCTTTTTGAAGTACTTTGCGGGGTTAACAAGTCAGAGAAAGTTGAAGAAGTTGCTCCTGAGATTATTGCAGCCGCTAGAGATGTCCAAGAGAAGACGGAAATATACGCCCCGTATAATATTCTTCCCTTGGATTCTGCTGGTGCTACACAGTCTGTCATGCAGCTTGACGAGGTTAAGGCCGCTGTGGCTGCACTGTGGAATACTCGTGGTTTAAACTGGCCAAGTGCATTCGAGCAGCACCGACAAAAAGCTAATGATTTGGATCTCCTTGATTGGCTCAGGGCCATGTTTGGGTTCCAGAGAGATAACGTCAGGAACCAGAGGGAGCATTTGATATTGCTACTTGCGAATACGCATATAAGACTTCATCCCAAACCTGAACCTCTTAACAAGCTTGATGATCGAGCTGTTGATGCAGTTCTGGACAAGCTTTTTAAGAACTACAAAACGTGGTGTAAATTTTTGGGTCGAAAACATAGTTTAAGGCTACCTCAAGGTCAGCAAGAAGTACAACAGAGGAAGATACTTTACATGGGTTTATATCTTCTCATATGGGGTGAAGCAGCGAATGTTCGCTTCATGCCTGAATGCCTCTGCTATATTTTCCATAATATGGCATATGAACTCCATGGTCTCTTGGCTGGAAATGTCAGCATTGTCACTGGGGAAAATATAAAGCCTTCTTATGGTGGGGATGATGAGGCCTTTTTGAGGAAGGTTATAACCCCCCTATACCGTGTAATCGAAAAGGAAGCCAAGAAGGGCGGCGGTGGAAAAGCTCCTCACACAGCTTGGTGCAACTATGATGATCTAAATGAATATTTCTGGTCATCTGATTGTTTCTCTCTTGGTTGGCCCATGCGTGATGATGGGGATTTTTTTAAATCAACTCGTGACTTGACACAGGGAAGAAAGGGCTCTAAAAGAAAATCTGGAAGCACAGGCAAATCATATTTTATTGAAACTCGCACATTTTGGCATATCTTTCGAAGTTTTGATCGAATGTGGACCTTTTATATACTGGCTCTACAGGCTATTCTCATTATTGCATTCAGCAAGGTTTCACCGCTGGATATATTTCAACAGGATGTCCTAAAAGATCTATCAAGTATTTTCATTACAGCAGCATTTCTTCGCGTCCTTCAGAGTATTCTGGATATGGTTTTGAACTTCCCTGGATATCACAGGTGTCGGTTCACAGATGTCCTGAGAAATATTCTGAAGATAGTTGTTAGTCTTGCATGGGCAGTCATTCTTCCACTTTTCTATGTGCATTCCTTCAATGATTCCCCTCAACAAATTGCAGATATACTGTCATTCCTTAAGAACATTCAAGGCGTTCCATCATTATATCTATTGGCTGTTGCAGTATATTTGCTTCCAAATTTACTGACAGCAGTTTTGTTCCTTTTCCCAATGCTCCGTCGTTTTATTGAAAATTCAGACTGGCATATCATCAGGTTCCTCTTGTGGTGGTCACAGCCAAGAATATATGTTGGAAGAGGAATGCATGAAAGTCAATTTGCCCTTCTAAAGTATACTTTTTTCTGGTTGCTACTTTTGGCTTGCAAGTTTACAGTTAGCTATTTTGTCCAGATAAAACCTCTGGTAGAGCCAACAGAAGACATAATGAAGATTCGTCGTATTAATTATGAGTGGCATGAATTTTTCCCTAATGGTACTCATTTCTATTGCTCCCATGTTTTTGTGAACTTCAACTTTCGGGTTTATTTCATGGACACACAAATTTGGTATGCTATATTTCAAACATTATATGGAGGTATTGTTGGCGCCTTTGATCGCCTCGGAGAGATTCGTACTCTAGGCATGCTAAGATCCCGGTTCCAGTCTCTACCTGGTGCATTCAACACATATCTAGTGCCCTCGGATAAGGAAATGGATTTGCTGCTGGTTCCTTATTCTTCAGATATGAGCCTGAAAATAATTCAGTGGCCACCTTTCTTGCTTGCTAGCAAAATCCCAATAGCATTAGATATGGCAGCTCAATTCAAAAACAAGGACTCTGATCTCTGGAAGCGGATATGTGGAGATGAATATATGAAGTGTGCTGTGATTGAATGTTATGAGTCTTTCAAACATGTCCTTAAGGCTTTGGTGGTTGGAGAAAATGAGAAAAGATTATTCAAGAACAATAGTGAATTTTATCTTACTGTGCTAGTAAATATTCTGATTTGCTTATCATTTGACAGGATCATTGGCATCATTATTAAGGAAATTGAAAGTAACATTTCCAAGAATACATTTCTTGATAATTTTAGAATGGCCTCTTTACCCACTCTCTGCCAGAAATTTGTAGAGCTTGTGGTGATCTTGAAAGATGGTAATTCATCCAAGCGATCTGCGGTGGTGCTGCTGCTGCAAGATATGTTGGAAGTTGTCACTCGTGATATGATGGTGAATGAGATCCGGTTGGTGGTTGAAGTTGGTCATAGTAGCAAGGACACTGGAAGGCAACTTTTTGCTGGTACTGATGCAAAACCTGCGATCGTGTTCCCTCCTCCAGTTACAGCACAATGGGAAGAACAGATAAGGCGCCTCCATCTGCTGTTGACTGTTAAGGAGTCTGCAATTGATGTACCAATAAACCTTGAAGCACGTAGAAGGATTTCATTCTTTGCAAATTCATTGTTCATGGATATGCCCCGTGCTCCCCGTGTTCGTAAGATGCTTTCGTTCAGCATTTTGACTCCCTACTATAGTGAGGAGACTGTGTATTCCAAAAGTGACCTTGAGATGGAAAATGAAGATGGTGTATCAATCATATATTACTTGCAGAAGATTTTCCCAGATGAATGGACTAACTTCATGGAGCGGCTTAATTGTAAAAAGGATGCTGAAATATGGGAAAATGAAGAGAACATTTTGCAGCTGCGTCATTGGGTCTCCTTACGAGGACAAACTCTCTGCAGGACAGTTAGAGGAATGATGTATTACCGACGAGCTCTGAAGCTTCAGGCTTTTCTTGATATGGCTAATGAAGAAGAGATACTAGAAGGGTACAAAGCCGTCACAGTTTCACCAGAGGACGAAAAGAAGAGCCGGAGATCCCTATTTGCTCAACTAGAAGCAGTGGCTGATCTTAAATTTACCTATGTTGCTACTTGCCAAAACTATGGCAATCAGAAGCGAAGTGGAGATCGTCGTGCAACTGACATCCTGAATCTGATGGTTAATAATCCCTCTCTCAGGGTTGCATATATTGATGAAGTTGAAGAACGAGAAGGTGAAAAGGTGCAAAAGGTTTACTATTCTGTATTGGTCAAAGCAGTTGACAACCTTGACCAGGAAATATATCGTATAAAATTGCCAGGCTCAGCAAAGATTGGAGAAGGGAAGCCTGAAAACCAGAACCATGCAGTAATCTTTACTCGTGGAGAAGCTCTTCAGGCTATTGACATGAATCAGGACAATTACTTGGAAGAAGCTCTTAAGATGCGTAACCTTCTTGAAGAATTTAATGAGGATCATGGAGTGCGTTCGCCTTCAATTTTAGGTGTTCGTGAGCATATCTTTACTGGAAGTGTTTCTTCGTTGGCGTGGTTTATGTCAAATCAAGAAATGAGCTTCGTGACCATCGGTCAAAGAGTTCTTGCTAGACCTCTTAAGGTTCGGTTCCATTATGGACATCCAGATGTCTTTGATAGAATCTTCCATATTACTCGTGGTGGCATGAGCAAGGCTTCTCGTGGGATCAATCTCAGCGAGGATATTTTTGCAGGTTTCAATTCCACTCTAAGAAGAGGAAATGTCACTCACCATGAGTACATCCAAGTTGGGAAGGGTAGGGATGTTGGCCTCAATCAAATCTCTCTCTTTGAGGCTAAAGTTGCTTGCGGTAATGGAGAGCAGACACTTAGCAGAGACATCTATCGATTAGGGCACCGTTATGATTTCTTCCGCATGTTGTCGTATTACTTTTCAACAATAGGATTTTATATCAGCTCAATGTTGGTTGTGATGACGGTCTACTCTTTCCTGTATGGTAGACTCTACTTGTCATTGAGTGGAATGGAGAAGACAATTATAACCTATGCTGCTAGAAGGGGAAATAATGCTCTACAAACAGCTATGGCTTCACAATCTGTTGTCCAATTAGGTTTGTTGACGTCCTTGCCCATGATCATGGAAATCGGACTTGAAAGAGGGTTCAGAACTGCATTAGGGGACACGATAATAATGCAGCTGCAGCTAGCATCAGTTTTCTTTACTTTCTCACTGGGTACAAAGGTTCATTACTATGGGAGAACAATCCTGCATGGTGGGGCTAAGTACAGAGCAACTGGCCGTGGCTTTGTTGTTCGGCATGAAAGGTTTGGTGAAAACTACAGGATGTACTCACGGAGTCACTTTACAAAAGGCCTGGAGCTTATGATACTGCTTATTATTTATAGTATATATGGCTCAGCAGCAGATGGCTCAGCTGCATATATCTTCATCACATTCTCCATGTGGTTCTTAGTGATTTCTTGGTTATTTGCCCCCTTCCTCTTCAACCCTTCGGGATTTGAATGGCAAAAAATAGTGGAAGATTGGGATGATTGGTCAAAATGGATAACTAGCCGTGGTGGTATTGGTGTGCCAGCAAATAAGAGCTGGGAATCCTGGTGGGATGAGGAACAGGAGCACCTGCAGCACACTGGGATGTTGGGAAGGTTTTGGGAGATTGTACTTTCTCTGCGCTTCTTTCTTTTCCAATATGGAATTGTCTACCATCTCAATGTTTCCAGGGGTGATAAAAGTATCATGGTTTATGCTCTGTCATGGCTCGTCATTGTTGCTGTGATGATTATCCTCAAGATTGTATCCTTGGGTAGAAAAAGGTTCAGTGCTGATTTCCAGCTGATGTTCAGACTTCTGAAGTTGTTCGTCTTCATTGGGTTTGTCGTCACCATTGCAGTACTTTTTATTTTCCTTAGTCTCACTGTTGGTGACATCTTTGTAAGCTTACTGGCCTTCTTGCCTACTGGGTGGGCAATACTTATGATATCACAAGCATGCAGGCCATTGGTGAAGGTCTTAGGAATGTGGGGGTCTGTAAAAGCTCTAGCAAGAGGGTACGAGTACGTGATGGGACTGGTTATCTTTACCCCAGTGGCTGTTCTGGCATGGTTCCCATTTGTCTCAGAGTTCCAGACCAGGCTGCTATTCAACCAGGCTTTCAGTCGAGGCCTTCAGATCCAACGTATTCTTTCTGGTGGGAAGAAGCAAAAGTCCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006075 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009555 GO:0009556 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010208 GO:0010769 GO:0010927 GO:0016043 GO:0016049 GO:0016051 GO:0019953 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034293 GO:0034637 GO:0034645 GO:0040007 GO:0040008 GO:0043062 GO:0043170 GO:0043900 GO:0043934 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048236 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048646 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051273 GO:0051274 GO:0051321 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0080092 GO:0085029 GO:1901576 GO:1903046 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1893

Amino Acids

217.89

Weight (kDa)

9.01

Isoelectric Point (pI)

42.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vta1	PF04652	41 - 162	1.3e-12	Vta1 like
FKS1_dom1	PF14288	321 - 432	8.1e-41	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	799 - 862	2.3e-28	Callose synthase 1 domain
CALS1	PF25968	876 - 1006	2.7e-31	Callose synthase 1 domain
Glucan_synthase	PF02364	1014 - 1098	8e-27	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	1104 - 1707	4.7e-207	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0007588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13680
fragaria_vesca	FvH4_1g24300
malus_domestica	MD01G1004000.v1.1 MD01G1004100.v1.1 MD02G1211500.v1.1
prunus_persica	Prupe.4G271000_v2.0.a1
pyrus_communis	pycom01g02040 pycom15g30380
rosa_chinensis	RchiOBHm_Chr2g0120781
rosa_laevigata	RLG00000018581 RLG00000018588
rosa_roxburghii	Rroxscaffold_2G00122840 Rroxscaffold_2G00122950
rosa_rugosa	Rorug02G0231300
rosa_samantha	Rh2AG287900 Rh2AG288000 Rh2AG288100 Rh2BG299200 Rh2BG299300 Rh2CG277200 Rh2CG277300 Rh2DG313100 Rh2DG313200
rosa_wichuraiana	Rw2G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1163, 4487
AarI	CACCTGC	1 cut(s)	5097
AasI	GACNNNNNNGTC	1 cut(s)	95
AatII	GACGTC	1 cut(s)	4571
Acc36I	ACCTGC	4 cut(s)	385, 2950, 4169, 5097
AccB1I	GGYRCC	3 cut(s)	2231, 2994, 4341
AccB7I	CCANNNNNTGG	1 cut(s)	1054
AccBSI	CCGCTC	2 cut(s)	130, 3274
AccI	GTMKAC	5 cut(s)	399, 1979, 4428, 4447, 5169
AccII	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
AclWI	GGATC	8 cut(s)	711, 2271, 2660, 2860, 3501, 3933, 4160, 5632
AcoI	YGGCCR	5 cut(s)	41, 235, 659, 2393, 4744
AcuI	CTGAAG	8 cut(s)	1568, 1682, 2349, 3398, 3428, 3830, 5339, 5618
AcyI	GRCGYC	3 cut(s)	2232, 2995, 4568
AfiI	CCNNNNNNNGG	9 cut(s)	17, 732, 1054, 1127, 1152, 2875, 3196, 3648, 3745
AflII	CTTAAG	4 cut(s)	1763, 2546, 3890, 4064
AflIII	ACRYGT	2 cut(s)	195, 2539
AhdI	GACNNNNNGTC	1 cut(s)	4038
Alw21I	GWGCWC	4 cut(s)	409, 3112, 3406, 5088
Alw26I	GTCTC	7 cut(s)	1079, 2295, 3161, 3349, 4317, 5384, 5587
AlwI	GGATC	8 cut(s)	711, 2271, 2660, 2860, 3501, 3933, 4160, 5632
AlwNI	CAGNNNCTG	3 cut(s)	31, 3536, 5558
Ama87I	CYCGRG	2 cut(s)	158, 309
ArsI	GACNNNNNNTTYG	4 cut(s)	55, 87, 1825, 1857
AseI	ATTAAT	1 cut(s)	2088
Asp700I	GAANNNNTTC	5 cut(s)	3067, 3242, 3843, 3985, 5324
AspLEI	GCGC	6 cut(s)	56, 165, 211, 2234, 2997, 5142
AspS9I	GGNCC	6 cut(s)	20, 28, 220, 723, 1296, 1449
AsuC2I	CCSGG	2 cut(s)	225, 2281
AsuII	TTCGAA	1 cut(s)	1429
AsuNHI	GCTAGC	2 cut(s)	2411, 4651
AvaI	CYCGRG	2 cut(s)	158, 309
AvaII	GGWCC	2 cut(s)	220, 1449
BaeGI	GKGCMC	2 cut(s)	2325, 4344
BaeI	ACNNNNGTAYC	2 cut(s)	4674, 4707
BalI	TGGCCA	2 cut(s)	661, 2395
BanI	GGYRCC	3 cut(s)	2231, 2994, 4341
BanII	GRGCYC	4 cut(s)	159, 409, 1361, 3406
BarI	GAAGNNNNNNTAC	6 cut(s)	953, 985, 1803, 1835, 3432, 3464
BauI	CACGAG	6 cut(s)	645, 1332, 2844, 3831, 4122, 4146
BbsI	GAAGAC	5 cut(s)	76, 539, 2070, 4484, 5323
Bbv12I	GWGCWC	4 cut(s)	409, 3112, 3406, 5088
BceAI	ACGGC	6 cut(s)	28, 56, 222, 3452, 4731, 5007
BcgI	CGANNNNNNTGC	4 cut(s)	1409, 1443, 4621, 4655
BciVI	GTATCC	1 cut(s)	5275
BclI	TGATCA	2 cut(s)	364, 4581
BcnI	CCSGG	2 cut(s)	225, 2281
BcoDI	GTCTC	7 cut(s)	1079, 2295, 3161, 3349, 4317, 5384, 5587
BfmI	CTRYAG	7 cut(s)	269, 1469, 3157, 3367, 4646, 4786, 5090
BfoI	RGCGCY	4 cut(s)	57, 166, 2235, 2998
BfrI	CTTAAG	4 cut(s)	1763, 2546, 3890, 4064
BfuAI	ACCTGC	4 cut(s)	385, 2950, 4169, 5097
BfuI	GTATCC	1 cut(s)	5275
BglI	GCCNNNNNGGC	2 cut(s)	27, 626
BglII	AGATCT	1 cut(s)	1540
BlpI	GCTNAGC	3 cut(s)	3775, 4864, 4879
BmcAI	AGTACT	2 cut(s)	453, 5361
Bme18I	GGWCC	2 cut(s)	220, 1449
BmeRI	GACNNNNNGTC	1 cut(s)	4038
BmeT110I	CYCGRG	2 cut(s)	158, 309
BmgT120I	GGNCC	6 cut(s)	20, 28, 220, 723, 1296, 1449
BmrI	ACTGGG	5 cut(s)	1113, 4688, 5109, 5436, 5546
BmsI	GCATC	9 cut(s)	302, 316, 844, 2670, 2923, 3117, 3283, 3885, 4664
BmtI	GCTAGC	2 cut(s)	2415, 4655
BmuI	ACTGGG	5 cut(s)	1113, 4688, 5109, 5436, 5546
BoxI	GACNNNNGTC	2 cut(s)	43, 80
BpiI	GAAGAC	5 cut(s)	76, 539, 2070, 4484, 5323
BpmI	CTGGAG	3 cut(s)	214, 2946, 4844
Bpu10I	CCTNAGC	1 cut(s)	719
Bpu1102I	GCTNAGC	3 cut(s)	3775, 4864, 4879
Bpu14I	TTCGAA	1 cut(s)	1429
BpuEI	CTTGAG	4 cut(s)	393, 918, 3212, 5240
BpuMI	CCSGG	2 cut(s)	225, 2281
Bsa29I	ATCGAT	1 cut(s)	4333
BsaAI	YACGTR	2 cut(s)	3059, 5527
BsaBI	GATNNNNATC	2 cut(s)	702, 5249
BsaHI	GRCGYC	3 cut(s)	2232, 2995, 4568
BsaI	GGTCTC	2 cut(s)	1079, 3349
BsaWI	WCCGGW	1 cut(s)	2868
BsaXI	ACNNNNNCTCC	6 cut(s)	212, 242, 2211, 2241, 3158, 3188
Bsc4I	CCNNNNNNNGG	9 cut(s)	17, 732, 1054, 1127, 1152, 2875, 3196, 3648, 3745
Bse3DI	GCAATG	1 cut(s)	5352
Bse8I	GATNNNNATC	2 cut(s)	702, 5249
BseCI	ATCGAT	1 cut(s)	4333
BseJI	GATNNNNATC	2 cut(s)	702, 5249
BseLI	CCNNNNNNNGG	9 cut(s)	17, 732, 1054, 1127, 1152, 2875, 3196, 3648, 3745
BseMI	GCAATG	1 cut(s)	5352
BseMII	CTCAG	9 cut(s)	489, 733, 1640, 3661, 3789, 4174, 4878, 4893, 5598
BseRI	GAGGAG	5 cut(s)	122, 404, 1212, 2949, 3179
BseSI	GKGCMC	2 cut(s)	2325, 4344
BseX3I	CGGCCG	2 cut(s)	41, 235
BseYI	CCCAGC	1 cut(s)	5055
BsgI	GTGCAG	1 cut(s)	615
Bsh1236I	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
Bsh1285I	CGRYCG	3 cut(s)	44, 238, 2949
BshNI	GGYRCC	3 cut(s)	2231, 2994, 4341
BshVI	ATCGAT	1 cut(s)	4333
BsiEI	CGRYCG	3 cut(s)	44, 238, 2949
BsiHKAI	GWGCWC	4 cut(s)	409, 3112, 3406, 5088
BsiHKCI	CYCGRG	2 cut(s)	158, 309
BsiSI	CCGG	5 cut(s)	18, 225, 2281, 2869, 3502
BslFI	GGGAC	3 cut(s)	86, 4640, 5547
BslI	CCNNNNNNNGG	9 cut(s)	17, 732, 1054, 1127, 1152, 2875, 3196, 3648, 3745
BsmAI	GTCTC	7 cut(s)	1079, 2295, 3161, 3349, 4317, 5384, 5587
BsmFI	GGGAC	3 cut(s)	86, 4640, 5547
BsmI	GAATGC	6 cut(s)	668, 1034, 1490, 1714, 1953, 2303
Bso31I	GGTCTC	2 cut(s)	1079, 3349
BsoBI	CYCGRG	2 cut(s)	158, 309
Bsp119I	TTCGAA	1 cut(s)	1429
Bsp1286I	GDGCHC	8 cut(s)	159, 409, 1361, 2325, 3112, 3406, 4344, 5088
Bsp1720I	GCTNAGC	3 cut(s)	3775, 4864, 4879
Bsp19I	CCATGG	1 cut(s)	1069
BspCNI	CTCAG	9 cut(s)	490, 732, 1641, 3660, 3788, 4173, 4877, 4892, 5597
BspDI	ATCGAT	1 cut(s)	4333
BspFNI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
BspMAI	CTGCAG	4 cut(s)	273, 3371, 4650, 5094
BspMI	ACCTGC	4 cut(s)	385, 2950, 4169, 5097
BspOI	GCTAGC	2 cut(s)	2415, 4655
BspPI	GGATC	8 cut(s)	711, 2271, 2660, 2860, 3501, 3933, 4160, 5632
BspQI	GCTCTTC	2 cut(s)	3491, 3849
BspT104I	TTCGAA	1 cut(s)	1429
BspT107I	GGYRCC	3 cut(s)	2231, 2994, 4341
BspTI	CTTAAG	4 cut(s)	1763, 2546, 3890, 4064
BspTNI	GGTCTC	2 cut(s)	1079, 3349
BsrBI	CCGCTC	2 cut(s)	130, 3274
BsrDI	GCAATG	1 cut(s)	5352
BssNAI	GTATAC	1 cut(s)	1980
BssNI	GRCGYC	3 cut(s)	2232, 2995, 4568
BssSI	CACGAG	6 cut(s)	645, 1332, 2844, 3831, 4122, 4146
BssT1I	CCWWGG	3 cut(s)	567, 1069, 5268
Bst1107I	GTATAC	1 cut(s)	1980
Bst2BI	CACGAG	6 cut(s)	645, 1332, 2844, 3831, 4122, 4146
Bst6I	CTCTTC	7 cut(s)	1936, 3309, 3438, 3491, 3849, 4195, 4955
BstACI	GRCGYC	3 cut(s)	2232, 2995, 4568
BstAFI	CTTAAG	4 cut(s)	1763, 2546, 3890, 4064
BstAPI	GCANNNNNTGC	1 cut(s)	2942
BstBAI	YACGTR	2 cut(s)	3059, 5527
BstBI	TTCGAA	1 cut(s)	1429
BstC8I	GCNNGC	9 cut(s)	2008, 2270, 2409, 2413, 4294, 4653, 5043, 5458, 5462
BstDSI	CCRYGG	4 cut(s)	238, 1069, 4747, 5023
BstENI	CCTNNNNNAGG	3 cut(s)	1150, 3646, 3743
BstFNI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
BstH2I	RGCGCY	4 cut(s)	57, 166, 2235, 2998
BstHHI	GCGC	6 cut(s)	56, 165, 211, 2234, 2997, 5142
BstMAI	GTCTC	7 cut(s)	1079, 2295, 3161, 3349, 4317, 5384, 5587
BstMCI	CGRYCG	3 cut(s)	44, 238, 2949
BstNSI	RCATGY	4 cut(s)	2272, 2543, 4366, 5460
BstPAI	GACNNNNGTC	2 cut(s)	43, 80
BstSFI	CTRYAG	7 cut(s)	269, 1469, 3157, 3367, 4646, 4786, 5090
BstSLI	GKGCMC	2 cut(s)	2325, 4344
BstUI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
BstV2I	GAAGAC	5 cut(s)	76, 539, 2070, 4484, 5323
BstX2I	RGATCY	6 cut(s)	703, 1540, 2276, 2865, 3506, 5637
BstXI	CCANNNNNNTGG	3 cut(s)	3255, 3575, 4585
BstYI	RGATCY	6 cut(s)	703, 1540, 2276, 2865, 3506, 5637
BstZ17I	GTATAC	1 cut(s)	1980
BstZI	CGGCCG	2 cut(s)	41, 235
Bsu15I	ATCGAT	1 cut(s)	4333
BsuI	GTATCC	1 cut(s)	5275
BsuTUI	ATCGAT	1 cut(s)	4333
BtgI	CCRYGG	4 cut(s)	238, 1069, 4747, 5023
BtgZI	GCGATG	1 cut(s)	97
BtsI	GCAGTG	1 cut(s)	3537
BveI	ACCTGC	4 cut(s)	385, 2950, 4169, 5097
Cac8I	GCNNGC	9 cut(s)	2008, 2270, 2409, 2413, 4294, 4653, 5043, 5458, 5462
CaiI	CAGNNNCTG	3 cut(s)	31, 3536, 5558
CfoI	GCGC	6 cut(s)	56, 165, 211, 2234, 2997, 5142
Cfr13I	GGNCC	6 cut(s)	20, 28, 220, 723, 1296, 1449
ClaI	ATCGAT	1 cut(s)	4333
CseI	GACGC	5 cut(s)	43, 127, 160, 1567, 3323
CsiI	ACCWGGT	1 cut(s)	2296
CspCI	CAANNNNNGTGG	2 cut(s)	2385, 2420
DinI	GGCGCC	2 cut(s)	2233, 2996
DraI	TTTAAA	2 cut(s)	654, 1324
DrdI	GACNNNNNNGTC	1 cut(s)	95
DriI	GACNNNNNGTC	1 cut(s)	4038
DseDI	GACNNNNNNGTC	1 cut(s)	95
EaeI	YGGCCR	5 cut(s)	41, 235, 659, 2393, 4744
EagI	CGGCCG	2 cut(s)	41, 235
Eam1104I	CTCTTC	7 cut(s)	1936, 3309, 3438, 3491, 3849, 4195, 4955
Eam1105I	GACNNNNNGTC	1 cut(s)	4038
EarI	CTCTTC	7 cut(s)	1936, 3309, 3438, 3491, 3849, 4195, 4955
EciI	GGCGGA	1 cut(s)	97
Ecl136II	GAGCTC	2 cut(s)	407, 3404
EclXI	CGGCCG	2 cut(s)	41, 235
Eco130I	CCWWGG	3 cut(s)	567, 1069, 5268
Eco147I	AGGCCT	3 cut(s)	1145, 4821, 5631
Eco24I	GRGCYC	4 cut(s)	159, 409, 1361, 3406
Eco31I	GGTCTC	2 cut(s)	1079, 3349
Eco32I	GATATC	2 cut(s)	1622, 5448
Eco47I	GGWCC	2 cut(s)	220, 1449
Eco52I	CGGCCG	2 cut(s)	41, 235
Eco53kI	GAGCTC	2 cut(s)	407, 3404
Eco57I	CTGAAG	8 cut(s)	1568, 1682, 2349, 3398, 3428, 3830, 5339, 5618
Eco88I	CYCGRG	2 cut(s)	158, 309
EcoICRI	GAGCTC	2 cut(s)	407, 3404
EcoNI	CCTNNNNNAGG	3 cut(s)	1150, 3646, 3743
EcoO109I	RGGNCCY	1 cut(s)	28
EcoRV	GATATC	2 cut(s)	1622, 5448
EcoT14I	CCWWGG	3 cut(s)	567, 1069, 5268
EcoT22I	ATGCAT	2 cut(s)	295, 1953
EcoT38I	GRGCYC	4 cut(s)	159, 409, 1361, 3406
EgeI	GGCGCC	2 cut(s)	2233, 2996
EheI	GGCGCC	2 cut(s)	2233, 2996
ErhI	CCWWGG	3 cut(s)	567, 1069, 5268
FalI	AAGNNNNNCTT	4 cut(s)	3873, 3905, 3973, 4005
FaqI	GGGAC	3 cut(s)	86, 4640, 5547
FauI	CCCGC	1 cut(s)	452
FauNDI	CATATG	2 cut(s)	992, 1060
FbaI	TGATCA	2 cut(s)	364, 4581
FblI	GTMKAC	5 cut(s)	399, 1979, 4428, 4447, 5169
FriOI	GRGCYC	4 cut(s)	159, 409, 1361, 3406
GlaI	GCGC	6 cut(s)	55, 164, 210, 2233, 2996, 5141
GsaI	CCCAGC	1 cut(s)	5059
GsuI	CTGGAG	3 cut(s)	214, 2946, 4844
HaeII	RGCGCY	4 cut(s)	57, 166, 2235, 2998
HapII	CCGG	5 cut(s)	18, 225, 2281, 2869, 3502
HgaI	GACGC	5 cut(s)	43, 127, 160, 1567, 3323
HhaI	GCGC	6 cut(s)	56, 165, 211, 2234, 2997, 5142
Hin1I	GRCGYC	3 cut(s)	2232, 2995, 4568
Hin6I	GCGC	6 cut(s)	54, 163, 209, 2232, 2995, 5140
HinP1I	GCGC	6 cut(s)	54, 163, 209, 2232, 2995, 5140
HincII	GTYRAC	4 cut(s)	466, 3012, 3733, 4566
HindII	GTYRAC	4 cut(s)	466, 3012, 3733, 4566
HindIII	AAGCTT	5 cut(s)	343, 833, 869, 3410, 5404
HpaI	GTTAAC	1 cut(s)	466
HpaII	CCGG	5 cut(s)	18, 225, 2281, 2869, 3502
HpyCH4IV	ACGT	7 cut(s)	68, 750, 890, 3058, 4568, 5526, 5644
HpySE526I	ACGT	7 cut(s)	68, 750, 890, 3058, 4568, 5526, 5644
Hsp92I	GRCGYC	3 cut(s)	2232, 2995, 4568
HspAI	GCGC	6 cut(s)	54, 163, 209, 2232, 2995, 5140
KasI	GGCGCC	2 cut(s)	2231, 2994
Ksp22I	TGATCA	2 cut(s)	364, 4581
KspAI	GTTAAC	1 cut(s)	466
LguI	GCTCTTC	2 cut(s)	3491, 3849
LweI	GCATC	9 cut(s)	302, 316, 844, 2670, 2923, 3117, 3283, 3885, 4664
MabI	ACCWGGT	1 cut(s)	2296
MaeII	ACGT	7 cut(s)	68, 750, 890, 3058, 4568, 5526, 5644
MbiI	CCGCTC	2 cut(s)	130, 3274
MfeI	CAATTG	1 cut(s)	3030
MflI	RGATCY	6 cut(s)	703, 1540, 2276, 2865, 3506, 5637
MhlI	GDGCHC	8 cut(s)	159, 409, 1361, 2325, 3112, 3406, 4344, 5088
MlsI	TGGCCA	2 cut(s)	661, 2395
MluI	ACGCGT	1 cut(s)	195
MluNI	TGGCCA	2 cut(s)	661, 2395
Mly113I	GGCGCC	2 cut(s)	2232, 2995
MlyI	GAGTC	6 cut(s)	2456, 2537, 3032, 3142, 4443, 4813
MmeI	TCCRAC	4 cut(s)	1918, 2542, 2811, 5665
Mox20I	TGGCCA	2 cut(s)	661, 2395
Mph1103I	ATGCAT	2 cut(s)	295, 1953
MroXI	GAANNNNTTC	5 cut(s)	3067, 3242, 3843, 3985, 5324
MscI	TGGCCA	2 cut(s)	661, 2395
MslI	CAYNNNNRTG	8 cut(s)	3098, 4092, 4347, 4583, 4698, 5102, 5178, 5455
Msp20I	TGGCCA	2 cut(s)	661, 2395
MspA1I	CMGCKG	7 cut(s)	234, 623, 1510, 3331, 4645, 4883, 5302
MspCI	CTTAAG	4 cut(s)	1763, 2546, 3890, 4064
MspI	CCGG	5 cut(s)	18, 225, 2281, 2869, 3502
MssI	GTTTAAAC	1 cut(s)	654
MunI	CAATTG	1 cut(s)	3030
Mva1269I	GAATGC	6 cut(s)	668, 1034, 1490, 1714, 1953, 2303
MvnI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1578
NarI	GGCGCC	2 cut(s)	2232, 2995
NciI	CCSGG	2 cut(s)	225, 2281
NcoI	CCATGG	1 cut(s)	1069
NdeI	CATATG	2 cut(s)	992, 1060
NheI	GCTAGC	2 cut(s)	2411, 4651
NsiI	ATGCAT	2 cut(s)	295, 1953
NspI	RCATGY	4 cut(s)	2272, 2543, 4366, 5460
NspV	TTCGAA	1 cut(s)	1429
PaeI	GCATGC	2 cut(s)	2272, 5460
PaeR7I	CTCGAG	1 cut(s)	309
PaqCI	CACCTGC	1 cut(s)	5097
PasI	CCCWGGG	1 cut(s)	25
PceI	AGGCCT	3 cut(s)	1145, 4821, 5631
PciI	ACATGT	1 cut(s)	2539
PciSI	GCTCTTC	2 cut(s)	3491, 3849
PcsI	WCGNNNNNNNCGW	2 cut(s)	41, 71
PctI	GAATGC	6 cut(s)	668, 1034, 1490, 1714, 1953, 2303
PdmI	GAANNNNTTC	5 cut(s)	3067, 3242, 3843, 3985, 5324
PfeI	GAWTC	9 cut(s)	14, 572, 1727, 2077, 2254, 3622, 3862, 4107, 5060
PflMI	CCANNNNNTGG	1 cut(s)	1054
Ple19I	CGATCG	1 cut(s)	2949
PleI	GAGTC	6 cut(s)	2456, 2536, 3031, 3142, 4443, 4812
PluTI	GGCGCC	2 cut(s)	2235, 2998
PmeI	GTTTAAAC	1 cut(s)	654
PpsI	GAGTC	6 cut(s)	2456, 2536, 3031, 3142, 4443, 4812
Ppu21I	YACGTR	2 cut(s)	3059, 5527
PscI	ACATGT	1 cut(s)	2539
PshAI	GACNNNNGTC	2 cut(s)	43, 80
PshBI	ATTAAT	1 cut(s)	2088
PsiI	TTATAA	2 cut(s)	1163, 4487
Psp124BI	GAGCTC	2 cut(s)	409, 3406
PspFI	CCCAGC	1 cut(s)	5055
PspPI	GGNCC	6 cut(s)	20, 28, 220, 723, 1296, 1449
PspXI	VCTCGAGB	1 cut(s)	309
PstI	CTGCAG	4 cut(s)	273, 3371, 4650, 5094
PstNI	CAGNNNCTG	3 cut(s)	31, 3536, 5558
PsuI	RGATCY	6 cut(s)	703, 1540, 2276, 2865, 3506, 5637
PvuI	CGATCG	1 cut(s)	2949
PvuII	CAGCTG	4 cut(s)	3331, 4645, 4883, 5302
RseI	CAYNNNNRTG	8 cut(s)	3098, 4092, 4347, 4583, 4698, 5102, 5178, 5455
SacI	GAGCTC	2 cut(s)	409, 3406
SapI	GCTCTTC	2 cut(s)	3491, 3849
Sau96I	GGNCC	6 cut(s)	20, 28, 220, 723, 1296, 1449
ScaI	AGTACT	2 cut(s)	453, 5361
SchI	GAGTC	6 cut(s)	2456, 2537, 3032, 3142, 4443, 4813
SduI	GDGCHC	8 cut(s)	159, 409, 1361, 2325, 3112, 3406, 4344, 5088
SexAI	ACCWGGT	1 cut(s)	2296
SfaNI	GCATC	9 cut(s)	302, 316, 844, 2670, 2923, 3117, 3283, 3885, 4664
SfcI	CTRYAG	7 cut(s)	269, 1469, 3157, 3367, 4646, 4786, 5090
SfiI	GGCCNNNNNGGCC	1 cut(s)	27
SfoI	GGCGCC	2 cut(s)	2233, 2996
Sfr274I	CTCGAG	1 cut(s)	309
SfuI	TTCGAA	1 cut(s)	1429
SinI	GGWCC	2 cut(s)	220, 1449
SlaI	CTCGAG	1 cut(s)	309
SmiMI	CAYNNNNRTG	8 cut(s)	3098, 4092, 4347, 4583, 4698, 5102, 5178, 5455
SmlI	CTYRAG	9 cut(s)	309, 408, 933, 1763, 2546, 3191, 3890, 4064, 5255
SmoI	CTYRAG	9 cut(s)	309, 408, 933, 1763, 2546, 3191, 3890, 4064, 5255
SphI	GCATGC	2 cut(s)	2272, 5460
SseBI	AGGCCT	3 cut(s)	1145, 4821, 5631
SspDI	GGCGCC	2 cut(s)	2231, 2994
SspI	AATATT	5 cut(s)	359, 559, 1262, 1657, 2626
SstI	GAGCTC	2 cut(s)	409, 3406
StuI	AGGCCT	3 cut(s)	1145, 4821, 5631
StyI	CCWWGG	3 cut(s)	567, 1069, 5268
TaiI	ACGT	7 cut(s)	71, 753, 893, 3061, 4571, 5529, 5647
TaqII	GACCGA	1 cut(s)	4028
TatI	WGTACW	7 cut(s)	451, 949, 4224, 4731, 4794, 5128, 5359
TauI	GCSGC	7 cut(s)	8, 59, 237, 515, 623, 1209, 3277
TfiI	GAWTC	9 cut(s)	14, 572, 1727, 2077, 2254, 3622, 3862, 4107, 5060
TspGWI	ACGGA	5 cut(s)	64, 92, 551, 1853, 4816
Van91I	CCANNNNNTGG	1 cut(s)	1054
Vha464I	CTTAAG	4 cut(s)	1763, 2546, 3890, 4064
VpaK11BI	GGWCC	2 cut(s)	220, 1449
VspI	ATTAAT	1 cut(s)	2088
XagI	CCTNNNNNAGG	3 cut(s)	1150, 3646, 3743
XceI	RCATGY	4 cut(s)	2272, 2543, 4366, 5460
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2294, 4121
XhoI	CTCGAG	1 cut(s)	309
XmiI	GTMKAC	5 cut(s)	399, 1979, 4428, 4447, 5169
XmnI	GAANNNNTTC	5 cut(s)	3067, 3242, 3843, 3985, 5324
ZraI	GACGTC	1 cut(s)	4569
ZrmI	AGTACT	2 cut(s)	453, 5361
Zsp2I	ATGCAT	2 cut(s)	295, 1953

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.