Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0007588

Physical Location & Seq

Genomic Coordinates Reverse (-)

32158880 .. 32173275

Gene Structure 14396 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018581

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5604 bp

ATGGCGGCTCCAGATTCCGGCCCCCAGGGGCTGACGCGACGGCCGTCACGGAGCGCCGCGACGACGACGTTTTCGACGGAGGTCTTCGACAACGAGGTCGTCCCCTCTGCCATCGCCCCTATCGCTCCGCTCCTCCGCGTTGCAAACGAAGTCGAGCCCGAGCGCCCTCGCGTCGCCTATCTCTGTCGGTTCTACGCGTTTGAGAAGGCGCACAGATTGGACCCTGGCTCCAGCGGCCGTGGTGTGAGGCAGTTCAAGACCTTGCTCCTGCAGCGATTGGAGAGGGAGAATGCATCAAGTCTTTCTGCTCGAGTTAAAAAGTCTGATGCCAGGGAAATTACAAGCTTCTATCAGCAATATTATGATCACTATGTTAAAGCTGTTCAAGAGGAGCAGGTAGACAGAGCTCAAGTGAGTAAAGCCTACAATACACTTAACCAGCTTTTTGAAGTACTTTGCGGGGTTAACAAGTCAGAGAAAGTTGAAGAAGTTGCTCCTGAGATTATTGCAGCCGCTAGAGATGTCCAAGAGAAGACGGAAATATACGCTCCATATAATATTCTTCCTTTGGATTCTGCTGGTGCTACACAGTCTGTCATGCAGCTTGACGAGGTTAAGGCCGCTGTGGCTGCACTGTGGAATACTCGTGGTTTGAACTGGCCAAGTGCATTCGAGCAGCACAGACAAAAAGCTAATGATTTGGATCTCCTTGATTGGCTTAGGGCCATGTTTGGGTTCCAGAGAGATAACGTCAGGAACCAGAGGGAGCATTTGATATTGCTACTTGCGAATACGCATATAAGACTTCATCCCAAACCTGAACCTCTTAACAAGCTTGATGATCGAGCTGTTGATGCAGTTCTGGACAAGCTTTTTAAGAACTACAAAACGTGGTGTAAATTTTTGGGTCGAAAACATAGTTTAAGGCTACCTCAAGGTCAGCAAGAAGTACAACAGAGGAAGATACTTTACATGGGTTTATATCTTCTCATATGGGGTGAAGCAGCGAATGTTCGCTTCATGCCTGAATGCCTCTGCTATATTTTCCATAATATGGCATATGAACTCCATGGTCTCTTGGCTGGAAATGTCAGCATTGTCACTGGGGAAAATATAAAGCCTTCTTATGGTGGGGATGATGAGGCCTTTTTGAGGAAGGTTATAACCCCCCTGTACCGTGTAATTGAAAAGGAAGCCAAGAAGGGCGGCGGTGGAAAAGCTCCTCACACAGCTTGGTGCAACTATGATGATCTAAATGAATATTTCTGGTCATCTGATTGTTTCTCTCTTGGTTGGCCCATGCGTGATGATGGGGATTTTTTTAAATCAACTCGTGACTTGGCACAGGGCTCTAAAAGAAAATCTGGAAGCACAGGCAAATCATATTTTATTGAAACTCGCACATTTTGGCACATCTTTCGAAGTTTTGATCGAATGTGGACCTTTTATATACTGGCTCTACAGGCTATGCTCATTATTGCATTCAGCAAGGTTTCACCGCTGGATATATTTCAACAGGATGTCCTAAAAGATCTATCAAGTATTTTCATTACAGCAGCATTTCTTCGCGTCCTTCAGAGTATTCTGGATATGGTTTTGAACTTCCCTGGATATCACAGGTGTCGGTTCACAGATGTCCTGAGAAATATTCTGAAGATAGTTGTTAGTCTTGCATGGGCAGTCATTCTTCCACTTTTCTATGTGCATTCCTTCAATGATTCCCCTCAACAAATTGCAGATATACTGTCATTCCTTAAGAACATTCAAGGCGTTCCATCATTATATCTATTGGCTGTTGCAGTATATTTGCTTCCAAATTTACTGACAGCAGTTTTGTTCCTTTTCCCAATGCTCCGTCGTTTTATTGAAAATTCGGACTGGCATATCATCAGGTTCCTCTTGTGGTGGTCACAGCCAAGAATATATGTTGGAAGAGGAATGCATGAAAGTCAATTTGCCCTTATAAAGTATACTTTTTTCTGGTTGCTACTTTTGGCTTGCAAGTTTACAGTTAGCTATTTTGTCCAGATAAAACCTCTGGTAGAGCCAACAGAAGACATAATGAAGATTCGTCGTATTAATTATGAGTGGCATGAATTTTTCCCTAATGCTGAACATAACTATGGAGCAGTTGTCTCACTCTGGGCACCTGTTATCATGGTTTATTTCATGGACACACAAATTTGGTATGCTATATTTCAAACATTATATGGAGGTATTGTTGGCGCCTTTGATCGCCTAGGAGAGATTCGTACTCTAGGCATGCTAAGATCCCGGTTCCAGTCTCTACCTGGTGCATTCAACACATATCTAGTGCCCTCGGATAAGGAAATGGATTTGCTGCTGGTTCCTTATTCTTCAGATACGAGCCTGAAAATAATTCAGTGGCCACCTTTCTTGCTTGCTAGCAAAATCCCAATAGCATTAGATATGGCAGCTCAATTCAAAAACAAGGACTCTGATCTCTGGAAGCGGATATGTGGAGATGAATATATGAAGTGTGCTGTGATTGAATGTTATGAGTCTTTCAAACATGTCCTTAAGGCTTTGGTGGTTGGAGAAAATGAGAAAAGGATCATTGGCATCATTATTAAGGAAATTGAAAGTAACATTTCCAAGAATACATTTCTTGATAATTTTAGAATGGCCTCTTTACCCACTCTCTGCCAGAAATTTGTAGAGCTTGTGGTGATCTTGAAAGATGGTGATTCATCCAAGCGATCTGCGGTGGTGCTGCTGCTGCAAGATATGTTGGAAGTTGTCACTCGTGATATGATGGTGAATGAGATCCGAGAGGTGGTTGAAGTTGGTCATAGTAGCAAGGACACTGGAAGGCAACTTTTTGCTGGTACTGATGCAAAACCTGCGATAGTGTTCCCTCCTCCAGTTACAGCACAATGGGAAGAACAGATAAGGCGCCTCCATCTGCTGTTGACTGTTAAGGAGTCTGCAATTGATGTACCAATAAACCTTGAAGCACGTAGAAGGATTTCATTCTTTGCAAATTCATTGTTCATGGATATGCCCCGTGCTCCCCGTGTTCGTAAGATGCTTTCGTTCAGCATTTTGACTCCCTACTATAGTGAGGAGACTGTGTATTCCAAAAGTGACCTTGAGATGGAAAATGAAGATGGTGTATCAATCATATATTACTTGCAGAAGATTTTCCCAGATGAATGGACTAACTTCATGGAGCGGCTTAATTGTAAAAAGGATTCTGAAATATGGGAAAATGAAGAGAACATTTTGCATCTGCGTCATTGGGTCTCCTTACGAGGACAAACTCTCTGCAGGACAGTTAGAGGAATGATGTATTACCGACGAGCTCTGAAGCTTCAGGCTTTTCTTGATATGGCTAATGAAGAAGAGATACTAGAAGGGTACAAAGCCGTCACAGTTTCACCAGAGGACGAAAAGAAGAGCCGGAGATCCCTATTTGCTCAACTAGAAGCAGTGGCTGATCTTAAATTTACCTACGTTGCTACTTGCCAAAACTACGGCAATCAGAAGCGAAGTGGAGATCGTCGTGCAACTGACATCCTGAATCTGATGGTTAATAATCCCTCTCTCAGGGTTGCATATATTGATGAAGTTGAAGAACGAGAAGGTGAAAAGGTGCAAAAGGTTTACTATTCTGTATTGGTCAAAGCAGTTGACAACCTTGACCAGGAAATATATCGTATAAAATTGCCGGGCTCAGCAAAGATTGGAGAAGGGAAGCCTGAAAACCAGAACCATGCAGTAGTCTTTACTCGTGGAGAAGCTCTTCAGGCTATTGACATGAATCAGGACAATTACTTGGAAGAAGCTCTTAAGATGCGTAACCTTCTTGAAGAATTTAATGAGGATCATGGAGTGCGTTCGCCTTCAATTTTAGGTGTTCGTGAGCATATCTTTACTGGAAGTGTTTCTTCGTTGGCGTGGTTTATGTCAAATCAAGAAATGAGCTTCGTGACCATCGGTCAAAGAGTTCTTGCTAGACCTCTTAAGGTTCGGTTCCATTATGGACATCCAGATGTCTTTGATAGAATCTTCCATATTACTCGTGGTGGCATGAGCAAGGCTTCTCGTGGGATCAATCTCAGCGAGGATATTTTTGCAGGTTTCAATTCCACTCTAAGAAGAGGAAATGTTACTCACCATGAGTACATCCAAGTTGGGAAGGGTAGGGATGTTGGCCTCAACCAAATCTCTCTCTTTGAGGCTAAAGTTGCTTGCGGTAATGGAGAGCAGACACTTAGCAGAGACATCTATCGATTAGGGCACCGTTATGATTTCTTCCGCATGTTGTCGTATTACTTTTCAACAATAGGATTTTATATCAGCTCAATGTTGGTTGTGATGACGGTCTACTCTTTCCTGTATGGTAGACTCTACTTGTCATTGAGTGGAATGGAGAAGACAATTGTGACCTATGCTGCCAGAAGGGGAAATAATGCTCTACAAACAGCTATGGCTTCACAATCTGTTGTCCAATTAGGTTTGTTGACGTCCTTGCCCATGATCATGGAAATCGGACTTGAAAGAGGGTTCAGAACTGCATTAGGGGACACGATAATAATGCAGCTGCAGCTAGCATCAGTTTTCTTTACTTTCTCACTGGGTACAAAGGTTCATTACTATGGGAGAACAATCCTGCATGGTGGGGCTAAGTACAGAGCAACCGGTCGTGGCTTTGTTGTTCGGCATGAAAGGTTTGGTGAAAACTACAGGATGTACTCACGGAGTCACTTTACAAAAGGCCTGGAGCTTATGATACTGCTTATTATTTATAGTATATATGGCTCAGCAGCAGATGGCTCAGCTGCATATATCTTCATCACATTCTCCATGTGGTTCTTAGTGATTTCTTGGTTATTTGCCCCCTTCCTCTTCAACCCTTCGGGATTTGAATGGCAAAAAATAGTGGAAGATTGGGATGATTGGTCAAAATGGATAACTAGCCGTGGTGGTATTGGTGTGCCAGCAAATAAGAGCTGGGAGTCCTGGTGGGATGAGGAACAGGAGCACCTGCAGCACACGGGGATGTTGGGACGGTTTTGGGAGATTGTACTTTCTCTGCGCTTCTTTCTTTTCCAATATGGAATTGTCTACCATCTCAATGTTTCCAGGGGTGACAAAAGTATCATGGTTTATGCTCTGTCATGGCTCGTCATTGTTGCTGTGATGATTATCCTCAAGATTGTATCCTTGGGTAGAAAAAGGTTCAGTGCTGATTTCCAGCTGATGTTCAGACTTCTGAAGCTGTTCGTCTTCATTGGGTTCGTCGTCACCATTGCAGTACTTTTTATTTTCCTTAGTCTCACTGTTGGTGACATCTTTGTAAGCTTACTGGCCTTCTTGCCTACTGGGTGGGCAATACTTATGATATCACAAGCATGCAGGCCATTGGTGAAGGTCGTGGGAATGTGGGGGTCTGTAAAAGCTCTAGCAAGAGGGTACGAGTACGTGATGGGACTGGTTATCTTTACCCCAGTGGCTGTTCTGGCATGGTTCCCATTTGTCTCAGAGTTCCAGACCAGGCTGCTATTCAACCAGGCTTTCAGTCGAGGCCTTCAGATCCAACGTATTCTTTCTGGTGGGAAGAAGCAAAAGTCCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006075 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009555 GO:0009556 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010208 GO:0010769 GO:0010927 GO:0016043 GO:0016049 GO:0016051 GO:0019953 GO:0022402 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0030154 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034293 GO:0034637 GO:0034645 GO:0040007 GO:0040008 GO:0043062 GO:0043170 GO:0043900 GO:0043934 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048236 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048646 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051273 GO:0051274 GO:0051321 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0071704 GO:0071840 GO:0080092 GO:0085029 GO:1901576 GO:1903046 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1868

Amino Acids

214.55

Weight (kDa)

8.89

Isoelectric Point (pI)

42.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vta1	PF04652	41 - 162	1.3e-12	Vta1 like
FKS1_dom1	PF14288	321 - 432	8e-41	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	797 - 980	9.4e-68	Callose synthase 1 domain
Glucan_synthase	PF02364	988 - 1073	6.4e-27	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	1078 - 1681	1.3e-206	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13680
fragaria_vesca	FvH4_1g24300
malus_domestica	MD01G1004000.v1.1 MD01G1004100.v1.1 MD02G1211500.v1.1
prunus_persica	Prupe.4G271000_v2.0.a1
pyrus_communis	pycom01g02040 pycom15g30380
rosa_chinensis	RchiOBHm_Chr2g0120781
rosa_laevigata	RLG00000018581 RLG00000018588
rosa_roxburghii	Rroxscaffold_2G00122840 Rroxscaffold_2G00122950
rosa_rugosa	Rorug02G0231300
rosa_samantha	Rh2AG287900 Rh2AG288000 Rh2AG288100 Rh2BG299200 Rh2BG299300 Rh2CG277200 Rh2CG277300 Rh2DG313100 Rh2DG313200
rosa_wichuraiana	Rw2G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1163, 1964
AarI	CACCTGC	1 cut(s)	5019
AasI	GACNNNNNNGTC	1 cut(s)	95
AatII	GACGTC	1 cut(s)	4493
Acc36I	ACCTGC	4 cut(s)	385, 2872, 4091, 5019
AccB1I	GGYRCC	4 cut(s)	2146, 2225, 2916, 4263
AccB7I	CCANNNNNTGG	1 cut(s)	1054
AccBSI	CCGCTC	2 cut(s)	130, 3196
AccI	GTMKAC	5 cut(s)	399, 1970, 4350, 4369, 5091
AccII	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1569
AclWI	GGATC	8 cut(s)	711, 2265, 2582, 2782, 3423, 3855, 4082, 5554
AcoI	YGGCCR	4 cut(s)	41, 235, 659, 2387
AcsI	RAATTY	9 cut(s)	899, 1816, 1870, 2096, 2181, 2672, 3004, 3467, 3836
AcuI	CTGAAG	8 cut(s)	1559, 1673, 2343, 3320, 3350, 3752, 5261, 5540
AcyI	GRCGYC	3 cut(s)	2226, 2917, 4490
AfiI	CCNNNNNNNGG	9 cut(s)	17, 732, 1054, 1127, 1152, 2797, 3118, 3570, 3667
AflII	CTTAAG	4 cut(s)	1754, 2540, 3812, 3986
AflIII	ACRYGT	2 cut(s)	195, 2533
AgeI	ACCGGT	1 cut(s)	4664
AhdI	GACNNNNNGTC	1 cut(s)	3960
Alw21I	GWGCWC	4 cut(s)	409, 3034, 3328, 5010
Alw26I	GTCTC	8 cut(s)	1079, 2140, 2289, 3083, 3271, 4239, 5306, 5509
AlwI	GGATC	8 cut(s)	711, 2265, 2582, 2782, 3423, 3855, 4082, 5554
AlwNI	CAGNNNCTG	3 cut(s)	31, 3458, 5480
Ama87I	CYCGRG	2 cut(s)	158, 309
ApoI	RAATTY	9 cut(s)	899, 1816, 1870, 2096, 2181, 2672, 3004, 3467, 3836
ArsI	GACNNNNNNTTYG	4 cut(s)	55, 87, 1816, 1848
AseI	ATTAAT	1 cut(s)	2079
AsiGI	ACCGGT	1 cut(s)	4664
Asp700I	GAANNNNTTC	5 cut(s)	2989, 3164, 3765, 3907, 5246
AspA2I	CCTAGG	1 cut(s)	2239
AspLEI	GCGC	6 cut(s)	56, 165, 211, 2228, 2919, 5064
AspS9I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1440
AsuC2I	CCSGG	2 cut(s)	2275, 3693
AsuII	TTCGAA	1 cut(s)	1420
AsuNHI	GCTAGC	2 cut(s)	2405, 4573
AvaI	CYCGRG	2 cut(s)	158, 309
AvaII	GGWCC	2 cut(s)	220, 1440
AvrII	CCTAGG	1 cut(s)	2239
BaeGI	GKGCMC	3 cut(s)	2149, 2319, 4266
BaeI	ACNNNNGTAYC	4 cut(s)	4596, 4629, 5107, 5140
BalI	TGGCCA	2 cut(s)	661, 2389
BanI	GGYRCC	4 cut(s)	2146, 2225, 2916, 4263
BanII	GRGCYC	5 cut(s)	159, 409, 1352, 3328, 3698
BarI	GAAGNNNNNNTAC	6 cut(s)	953, 985, 1794, 1826, 3354, 3386
BauI	CACGAG	6 cut(s)	645, 1332, 2766, 3753, 4044, 4068
BbsI	GAAGAC	5 cut(s)	76, 539, 2061, 4406, 5245
Bbv12I	GWGCWC	4 cut(s)	409, 3034, 3328, 5010
BceAI	ACGGC	6 cut(s)	28, 56, 222, 3374, 3514, 4929
BcgI	CGANNNNNNTGC	4 cut(s)	1400, 1434, 4543, 4577
BciVI	GTATCC	1 cut(s)	5197
BclI	TGATCA	2 cut(s)	364, 4503
BcnI	CCSGG	2 cut(s)	2275, 3693
BcoDI	GTCTC	8 cut(s)	1079, 2140, 2289, 3083, 3271, 4239, 5306, 5509
BfmI	CTRYAG	7 cut(s)	269, 1460, 3079, 3289, 4568, 4708, 5012
BfoI	RGCGCY	4 cut(s)	57, 166, 2229, 2920
BfrI	CTTAAG	4 cut(s)	1754, 2540, 3812, 3986
BfuAI	ACCTGC	4 cut(s)	385, 2872, 4091, 5019
BfuI	GTATCC	1 cut(s)	5197
BglI	GCCNNNNNGGC	1 cut(s)	626
BglII	AGATCT	1 cut(s)	1531
BlnI	CCTAGG	1 cut(s)	2239
BlpI	GCTNAGC	3 cut(s)	3697, 4786, 4801
BmcAI	AGTACT	2 cut(s)	453, 5283
Bme18I	GGWCC	2 cut(s)	220, 1440
BmeRI	GACNNNNNGTC	1 cut(s)	3960
BmeT110I	CYCGRG	2 cut(s)	158, 309
BmgT120I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1440
BmrI	ACTGGG	4 cut(s)	1113, 4610, 5358, 5468
BmsI	GCATC	9 cut(s)	302, 316, 844, 2592, 2845, 3039, 3259, 3807, 4586
BmtI	GCTAGC	2 cut(s)	2409, 4577
BmuI	ACTGGG	4 cut(s)	1113, 4610, 5358, 5468
BoxI	GACNNNNGTC	2 cut(s)	43, 80
BpiI	GAAGAC	5 cut(s)	76, 539, 2061, 4406, 5245
BpmI	CTGGAG	3 cut(s)	214, 2868, 4766
Bpu10I	CCTNAGC	1 cut(s)	719
Bpu1102I	GCTNAGC	3 cut(s)	3697, 4786, 4801
Bpu14I	TTCGAA	1 cut(s)	1420
BpuEI	CTTGAG	4 cut(s)	393, 918, 3134, 5162
BpuMI	CCSGG	2 cut(s)	2275, 3693
Bsa29I	ATCGAT	1 cut(s)	4255
BsaAI	YACGTR	2 cut(s)	2981, 5449
BsaBI	GATNNNNATC	2 cut(s)	702, 5171
BsaHI	GRCGYC	3 cut(s)	2226, 2917, 4490
BsaI	GGTCTC	2 cut(s)	1079, 3271
BsaWI	WCCGGW	1 cut(s)	4664
Bsc4I	CCNNNNNNNGG	9 cut(s)	17, 732, 1054, 1127, 1152, 2797, 3118, 3570, 3667
Bse118I	RCCGGY	1 cut(s)	4664
Bse3DI	GCAATG	1 cut(s)	5274
Bse8I	GATNNNNATC	2 cut(s)	702, 5171
BseCI	ATCGAT	1 cut(s)	4255
BseJI	GATNNNNATC	2 cut(s)	702, 5171
BseLI	CCNNNNNNNGG	9 cut(s)	17, 732, 1054, 1127, 1152, 2797, 3118, 3570, 3667
BseMI	GCAATG	1 cut(s)	5274
BseMII	CTCAG	8 cut(s)	489, 1631, 3583, 3711, 4096, 4800, 4815, 5520
BseRI	GAGGAG	5 cut(s)	122, 404, 1212, 2871, 3101
BseSI	GKGCMC	3 cut(s)	2149, 2319, 4266
BseX3I	CGGCCG	2 cut(s)	41, 235
BseYI	CCCAGC	1 cut(s)	4977
BsgI	GTGCAG	1 cut(s)	615
Bsh1236I	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1569
Bsh1285I	CGRYCG	3 cut(s)	44, 238, 4669
BshNI	GGYRCC	4 cut(s)	2146, 2225, 2916, 4263
BshTI	ACCGGT	1 cut(s)	4664
BshVI	ATCGAT	1 cut(s)	4255
BsiEI	CGRYCG	3 cut(s)	44, 238, 4669
BsiHKAI	GWGCWC	4 cut(s)	409, 3034, 3328, 5010
BsiHKCI	CYCGRG	2 cut(s)	158, 309
BsiSI	CCGG	5 cut(s)	18, 2275, 3424, 3692, 4665
BslFI	GGGAC	4 cut(s)	86, 4562, 5046, 5469
BslI	CCNNNNNNNGG	9 cut(s)	17, 732, 1054, 1127, 1152, 2797, 3118, 3570, 3667
BsmAI	GTCTC	8 cut(s)	1079, 2140, 2289, 3083, 3271, 4239, 5306, 5509
BsmFI	GGGAC	4 cut(s)	86, 4562, 5046, 5469
BsmI	GAATGC	7 cut(s)	295, 668, 1034, 1481, 1705, 1944, 2297
Bso31I	GGTCTC	2 cut(s)	1079, 3271
BsoBI	CYCGRG	2 cut(s)	158, 309
Bsp119I	TTCGAA	1 cut(s)	1420
Bsp1720I	GCTNAGC	3 cut(s)	3697, 4786, 4801
Bsp19I	CCATGG	1 cut(s)	1069
BspCNI	CTCAG	8 cut(s)	490, 1632, 3582, 3710, 4095, 4799, 4814, 5519
BspDI	ATCGAT	1 cut(s)	4255
BspFNI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1569
BspMAI	CTGCAG	4 cut(s)	273, 3293, 4572, 5016
BspMI	ACCTGC	4 cut(s)	385, 2872, 4091, 5019
BspOI	GCTAGC	2 cut(s)	2409, 4577
BspPI	GGATC	8 cut(s)	711, 2265, 2582, 2782, 3423, 3855, 4082, 5554
BspQI	GCTCTTC	2 cut(s)	3413, 3771
BspT104I	TTCGAA	1 cut(s)	1420
BspT107I	GGYRCC	4 cut(s)	2146, 2225, 2916, 4263
BspTI	CTTAAG	4 cut(s)	1754, 2540, 3812, 3986
BspTNI	GGTCTC	2 cut(s)	1079, 3271
BsrBI	CCGCTC	2 cut(s)	130, 3196
BsrDI	GCAATG	1 cut(s)	5274
BsrFI	RCCGGY	1 cut(s)	4664
BssAI	RCCGGY	1 cut(s)	4664
BssNAI	GTATAC	1 cut(s)	1971
BssNI	GRCGYC	3 cut(s)	2226, 2917, 4490
BssSI	CACGAG	6 cut(s)	645, 1332, 2766, 3753, 4044, 4068
BssT1I	CCWWGG	3 cut(s)	1069, 2239, 5190
Bst1107I	GTATAC	1 cut(s)	1971
Bst2BI	CACGAG	6 cut(s)	645, 1332, 2766, 3753, 4044, 4068
Bst6I	CTCTTC	7 cut(s)	1927, 3231, 3360, 3413, 3771, 4117, 4877
BstACI	GRCGYC	3 cut(s)	2226, 2917, 4490
BstAFI	CTTAAG	4 cut(s)	1754, 2540, 3812, 3986
BstAPI	GCANNNNNTGC	1 cut(s)	2864
BstBAI	YACGTR	2 cut(s)	2981, 5449
BstBI	TTCGAA	1 cut(s)	1420
BstC8I	GCNNGC	9 cut(s)	1999, 2264, 2403, 2407, 4216, 4575, 4965, 5380, 5384
BstDSI	CCRYGG	3 cut(s)	238, 1069, 4945
BstENI	CCTNNNNNAGG	3 cut(s)	1150, 3568, 3665
BstFNI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1569
BstH2I	RGCGCY	4 cut(s)	57, 166, 2229, 2920
BstHHI	GCGC	6 cut(s)	56, 165, 211, 2228, 2919, 5064
BstMAI	GTCTC	8 cut(s)	1079, 2140, 2289, 3083, 3271, 4239, 5306, 5509
BstMCI	CGRYCG	3 cut(s)	44, 238, 4669
BstNSI	RCATGY	4 cut(s)	2266, 2537, 4288, 5382
BstPAI	GACNNNNGTC	2 cut(s)	43, 80
BstSFI	CTRYAG	7 cut(s)	269, 1460, 3079, 3289, 4568, 4708, 5012
BstSLI	GKGCMC	3 cut(s)	2149, 2319, 4266
BstUI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1569
BstV2I	GAAGAC	5 cut(s)	76, 539, 2061, 4406, 5245
BstX2I	RGATCY	6 cut(s)	703, 1531, 2270, 2787, 3428, 5559
BstXI	CCANNNNNNTGG	2 cut(s)	3177, 4507
BstYI	RGATCY	6 cut(s)	703, 1531, 2270, 2787, 3428, 5559
BstZ17I	GTATAC	1 cut(s)	1971
BstZI	CGGCCG	2 cut(s)	41, 235
Bsu15I	ATCGAT	1 cut(s)	4255
BsuI	GTATCC	1 cut(s)	5197
BsuTUI	ATCGAT	1 cut(s)	4255
BtgI	CCRYGG	3 cut(s)	238, 1069, 4945
BtgZI	GCGATG	1 cut(s)	97
BtsI	GCAGTG	1 cut(s)	3459
BtsIMutI	CAGTG	9 cut(s)	632, 1101, 2390, 2826, 3459, 4598, 5215, 5304, 5481
BveI	ACCTGC	4 cut(s)	385, 2872, 4091, 5019
Cac8I	GCNNGC	9 cut(s)	1999, 2264, 2403, 2407, 4216, 4575, 4965, 5380, 5384
CaiI	CAGNNNCTG	3 cut(s)	31, 3458, 5480
CfoI	GCGC	6 cut(s)	56, 165, 211, 2228, 2919, 5064
Cfr10I	RCCGGY	1 cut(s)	4664
Cfr13I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1440
ClaI	ATCGAT	1 cut(s)	4255
CseI	GACGC	4 cut(s)	43, 160, 1558, 3245
CsiI	ACCWGGT	1 cut(s)	2290
CspAI	ACCGGT	1 cut(s)	4664
CspCI	CAANNNNNGTGG	2 cut(s)	2379, 2414
DinI	GGCGCC	2 cut(s)	2227, 2918
DraI	TTTAAA	1 cut(s)	1324
DrdI	GACNNNNNNGTC	1 cut(s)	95
DriI	GACNNNNNGTC	1 cut(s)	3960
DseDI	GACNNNNNNGTC	1 cut(s)	95
EaeI	YGGCCR	4 cut(s)	41, 235, 659, 2387
EagI	CGGCCG	2 cut(s)	41, 235
Eam1104I	CTCTTC	7 cut(s)	1927, 3231, 3360, 3413, 3771, 4117, 4877
Eam1105I	GACNNNNNGTC	1 cut(s)	3960
EarI	CTCTTC	7 cut(s)	1927, 3231, 3360, 3413, 3771, 4117, 4877
Ecl136II	GAGCTC	2 cut(s)	407, 3326
EclXI	CGGCCG	2 cut(s)	41, 235
Eco130I	CCWWGG	3 cut(s)	1069, 2239, 5190
Eco147I	AGGCCT	3 cut(s)	1145, 4743, 5553
Eco24I	GRGCYC	5 cut(s)	159, 409, 1352, 3328, 3698
Eco31I	GGTCTC	2 cut(s)	1079, 3271
Eco32I	GATATC	2 cut(s)	1613, 5370
Eco47I	GGWCC	2 cut(s)	220, 1440
Eco52I	CGGCCG	2 cut(s)	41, 235
Eco53kI	GAGCTC	2 cut(s)	407, 3326
Eco57I	CTGAAG	8 cut(s)	1559, 1673, 2343, 3320, 3350, 3752, 5261, 5540
Eco88I	CYCGRG	2 cut(s)	158, 309
EcoICRI	GAGCTC	2 cut(s)	407, 3326
EcoNI	CCTNNNNNAGG	3 cut(s)	1150, 3568, 3665
EcoRV	GATATC	2 cut(s)	1613, 5370
EcoT14I	CCWWGG	3 cut(s)	1069, 2239, 5190
EcoT22I	ATGCAT	2 cut(s)	295, 1944
EcoT38I	GRGCYC	5 cut(s)	159, 409, 1352, 3328, 3698
EgeI	GGCGCC	2 cut(s)	2227, 2918
EheI	GGCGCC	2 cut(s)	2227, 2918
ErhI	CCWWGG	3 cut(s)	1069, 2239, 5190
FalI	AAGNNNNNCTT	4 cut(s)	3795, 3827, 3895, 3927
FaqI	GGGAC	4 cut(s)	86, 4562, 5046, 5469
FauI	CCCGC	1 cut(s)	452
FauNDI	CATATG	2 cut(s)	992, 1060
FbaI	TGATCA	2 cut(s)	364, 4503
FblI	GTMKAC	5 cut(s)	399, 1970, 4350, 4369, 5091
FriOI	GRGCYC	5 cut(s)	159, 409, 1352, 3328, 3698
GlaI	GCGC	6 cut(s)	55, 164, 210, 2227, 2918, 5063
GsaI	CCCAGC	1 cut(s)	4981
GsuI	CTGGAG	3 cut(s)	214, 2868, 4766
HaeII	RGCGCY	4 cut(s)	57, 166, 2229, 2920
HapII	CCGG	5 cut(s)	18, 2275, 3424, 3692, 4665
HgaI	GACGC	4 cut(s)	43, 160, 1558, 3245
HhaI	GCGC	6 cut(s)	56, 165, 211, 2228, 2919, 5064
Hin1I	GRCGYC	3 cut(s)	2226, 2917, 4490
Hin6I	GCGC	6 cut(s)	54, 163, 209, 2226, 2917, 5062
HinP1I	GCGC	6 cut(s)	54, 163, 209, 2226, 2917, 5062
HincII	GTYRAC	4 cut(s)	466, 2934, 3655, 4488
HindII	GTYRAC	4 cut(s)	466, 2934, 3655, 4488
HindIII	AAGCTT	5 cut(s)	343, 833, 869, 3332, 5326
HpaI	GTTAAC	1 cut(s)	466
HpaII	CCGG	5 cut(s)	18, 2275, 3424, 3692, 4665
HpyCH4IV	ACGT	8 cut(s)	68, 750, 890, 2980, 3477, 4490, 5448, 5566
HpySE526I	ACGT	8 cut(s)	68, 750, 890, 2980, 3477, 4490, 5448, 5566
Hsp92I	GRCGYC	3 cut(s)	2226, 2917, 4490
HspAI	GCGC	6 cut(s)	54, 163, 209, 2226, 2917, 5062
KasI	GGCGCC	2 cut(s)	2225, 2916
Ksp22I	TGATCA	2 cut(s)	364, 4503
KspAI	GTTAAC	1 cut(s)	466
LguI	GCTCTTC	2 cut(s)	3413, 3771
LweI	GCATC	9 cut(s)	302, 316, 844, 2592, 2845, 3039, 3259, 3807, 4586
MabI	ACCWGGT	1 cut(s)	2290
MaeII	ACGT	8 cut(s)	68, 750, 890, 2980, 3477, 4490, 5448, 5566
MbiI	CCGCTC	2 cut(s)	130, 3196
MfeI	CAATTG	2 cut(s)	2952, 4404
MflI	RGATCY	6 cut(s)	703, 1531, 2270, 2787, 3428, 5559
MlsI	TGGCCA	2 cut(s)	661, 2389
MluI	ACGCGT	1 cut(s)	195
MluNI	TGGCCA	2 cut(s)	661, 2389
Mly113I	GGCGCC	2 cut(s)	2226, 2917
MlyI	GAGTC	7 cut(s)	2450, 2531, 2954, 3064, 4365, 4735, 4991
MmeI	TCCRAC	4 cut(s)	1909, 2536, 2733, 5587
Mox20I	TGGCCA	2 cut(s)	661, 2389
Mph1103I	ATGCAT	2 cut(s)	295, 1944
MroXI	GAANNNNTTC	5 cut(s)	2989, 3164, 3765, 3907, 5246
MscI	TGGCCA	2 cut(s)	661, 2389
MslI	CAYNNNNRTG	9 cut(s)	2121, 3020, 4014, 4269, 4505, 4620, 5024, 5100, 5377
Msp20I	TGGCCA	2 cut(s)	661, 2389
MspA1I	CMGCKG	6 cut(s)	234, 623, 1501, 4567, 4805, 5224
MspCI	CTTAAG	4 cut(s)	1754, 2540, 3812, 3986
MspI	CCGG	5 cut(s)	18, 2275, 3424, 3692, 4665
MunI	CAATTG	2 cut(s)	2952, 4404
Mva1269I	GAATGC	7 cut(s)	295, 668, 1034, 1481, 1705, 1944, 2297
MvnI	CGCG	6 cut(s)	37, 59, 138, 171, 197, 1569
NarI	GGCGCC	2 cut(s)	2226, 2917
NciI	CCSGG	2 cut(s)	2275, 3693
NcoI	CCATGG	1 cut(s)	1069
NdeI	CATATG	2 cut(s)	992, 1060
NheI	GCTAGC	2 cut(s)	2405, 4573
NsiI	ATGCAT	2 cut(s)	295, 1944
NspI	RCATGY	4 cut(s)	2266, 2537, 4288, 5382
NspV	TTCGAA	1 cut(s)	1420
PaeI	GCATGC	2 cut(s)	2266, 5382
PaeR7I	CTCGAG	1 cut(s)	309
PaqCI	CACCTGC	1 cut(s)	5019
PasI	CCCWGGG	1 cut(s)	25
PceI	AGGCCT	3 cut(s)	1145, 4743, 5553
PciI	ACATGT	1 cut(s)	2533
PciSI	GCTCTTC	2 cut(s)	3413, 3771
PcsI	WCGNNNNNNNCGW	2 cut(s)	41, 71
PctI	GAATGC	7 cut(s)	295, 668, 1034, 1481, 1705, 1944, 2297
PdmI	GAANNNNTTC	5 cut(s)	2989, 3164, 3765, 3907, 5246
PflMI	CCANNNNNTGG	1 cut(s)	1054
PinAI	ACCGGT	1 cut(s)	4664
PleI	GAGTC	7 cut(s)	2450, 2530, 2953, 3064, 4365, 4734, 4990
PluTI	GGCGCC	2 cut(s)	2229, 2920
PpsI	GAGTC	7 cut(s)	2450, 2530, 2953, 3064, 4365, 4734, 4990
Ppu21I	YACGTR	2 cut(s)	2981, 5449
PscI	ACATGT	1 cut(s)	2533
PshAI	GACNNNNGTC	2 cut(s)	43, 80
PshBI	ATTAAT	1 cut(s)	2079
PsiI	TTATAA	2 cut(s)	1163, 1964
Psp124BI	GAGCTC	2 cut(s)	409, 3328
PspFI	CCCAGC	1 cut(s)	4977
PspPI	GGNCC	5 cut(s)	20, 220, 723, 1296, 1440
PspXI	VCTCGAGB	1 cut(s)	309
PstI	CTGCAG	4 cut(s)	273, 3293, 4572, 5016
PstNI	CAGNNNCTG	3 cut(s)	31, 3458, 5480
PsuI	RGATCY	6 cut(s)	703, 1531, 2270, 2787, 3428, 5559
PvuII	CAGCTG	3 cut(s)	4567, 4805, 5224
RseI	CAYNNNNRTG	9 cut(s)	2121, 3020, 4014, 4269, 4505, 4620, 5024, 5100, 5377
SacI	GAGCTC	2 cut(s)	409, 3328
SapI	GCTCTTC	2 cut(s)	3413, 3771
Sau96I	GGNCC	5 cut(s)	20, 220, 723, 1296, 1440
ScaI	AGTACT	2 cut(s)	453, 5283
SchI	GAGTC	7 cut(s)	2450, 2531, 2954, 3064, 4365, 4735, 4991
SexAI	ACCWGGT	1 cut(s)	2290
SfaNI	GCATC	9 cut(s)	302, 316, 844, 2592, 2845, 3039, 3259, 3807, 4586
SfcI	CTRYAG	7 cut(s)	269, 1460, 3079, 3289, 4568, 4708, 5012
SfoI	GGCGCC	2 cut(s)	2227, 2918
Sfr274I	CTCGAG	1 cut(s)	309
SfuI	TTCGAA	1 cut(s)	1420
SinI	GGWCC	2 cut(s)	220, 1440
SlaI	CTCGAG	1 cut(s)	309
SmiMI	CAYNNNNRTG	9 cut(s)	2121, 3020, 4014, 4269, 4505, 4620, 5024, 5100, 5377
SmlI	CTYRAG	9 cut(s)	309, 408, 933, 1754, 2540, 3113, 3812, 3986, 5177
SmoI	CTYRAG	9 cut(s)	309, 408, 933, 1754, 2540, 3113, 3812, 3986, 5177
SphI	GCATGC	2 cut(s)	2266, 5382
SseBI	AGGCCT	3 cut(s)	1145, 4743, 5553
SspDI	GGCGCC	2 cut(s)	2225, 2916
SspI	AATATT	4 cut(s)	359, 559, 1262, 1648
SstI	GAGCTC	2 cut(s)	409, 3328
StuI	AGGCCT	3 cut(s)	1145, 4743, 5553
StyI	CCWWGG	3 cut(s)	1069, 2239, 5190
TaiI	ACGT	8 cut(s)	71, 753, 893, 2983, 3480, 4493, 5451, 5569
TaqII	GACCGA	1 cut(s)	3950
TatI	WGTACW	7 cut(s)	451, 949, 4146, 4653, 4716, 5050, 5281
TauI	GCSGC	7 cut(s)	8, 59, 237, 515, 623, 1209, 3199
TscAI	CASTG	9 cut(s)	639, 1108, 2390, 2833, 3459, 4605, 5215, 5311, 5481
TspGWI	ACGGA	5 cut(s)	64, 92, 551, 1844, 4738
TspRI	CASTG	9 cut(s)	639, 1108, 2390, 2833, 3459, 4605, 5215, 5311, 5481
Van91I	CCANNNNNTGG	1 cut(s)	1054
Vha464I	CTTAAG	4 cut(s)	1754, 2540, 3812, 3986
VpaK11BI	GGWCC	2 cut(s)	220, 1440
VspI	ATTAAT	1 cut(s)	2079
XagI	CCTNNNNNAGG	3 cut(s)	1150, 3568, 3665
XapI	RAATTY	9 cut(s)	899, 1816, 1870, 2096, 2181, 2672, 3004, 3467, 3836
XceI	RCATGY	4 cut(s)	2266, 2537, 4288, 5382
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2288, 4043
XhoI	CTCGAG	1 cut(s)	309
XmaJI	CCTAGG	1 cut(s)	2239
XmiI	GTMKAC	5 cut(s)	399, 1970, 4350, 4369, 5091
XmnI	GAANNNNTTC	5 cut(s)	2989, 3164, 3765, 3907, 5246
ZraI	GACGTC	1 cut(s)	4491
ZrmI	AGTACT	2 cut(s)	453, 5283
Zsp2I	ATGCAT	2 cut(s)	295, 1944

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.